KR102639490B1 - Manufacturing method of eco-friendly fruit tree disinfectant composition - Google Patents
Manufacturing method of eco-friendly fruit tree disinfectant composition Download PDFInfo
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- 239000000203 mixture Substances 0.000 title claims abstract description 46
- 239000000645 desinfectant Substances 0.000 title claims abstract description 36
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 33
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 201000010099 disease Diseases 0.000 claims abstract description 40
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 40
- 238000000855 fermentation Methods 0.000 claims abstract description 36
- 238000002156 mixing Methods 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 34
- 235000013336 milk Nutrition 0.000 claims abstract description 18
- 239000008267 milk Substances 0.000 claims abstract description 18
- 210000004080 milk Anatomy 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 235000000346 sugar Nutrition 0.000 claims abstract description 13
- 150000003839 salts Chemical class 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000011347 resin Substances 0.000 claims abstract description 8
- 229920005989 resin Polymers 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 12
- 235000020971 citrus fruits Nutrition 0.000 claims description 8
- 241000207199 Citrus Species 0.000 claims description 7
- 230000000844 anti-bacterial effect Effects 0.000 claims description 7
- 229940057070 sugarcane extract Drugs 0.000 claims description 7
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 6
- 235000011613 Pinus brutia Nutrition 0.000 claims description 6
- 241000018646 Pinus brutia Species 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 241000218691 Cupressaceae Species 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 4
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- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 20
- 239000004615 ingredient Substances 0.000 abstract description 12
- 230000001954 sterilising effect Effects 0.000 abstract description 10
- 235000011430 Malus pumila Nutrition 0.000 abstract description 7
- 235000015103 Malus silvestris Nutrition 0.000 abstract description 7
- 235000006040 Prunus persica var persica Nutrition 0.000 abstract description 5
- 235000014443 Pyrus communis Nutrition 0.000 abstract description 5
- 240000001987 Pyrus communis Species 0.000 abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 240000006413 Prunus persica var. persica Species 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 235000002639 sodium chloride Nutrition 0.000 description 9
- 239000000126 substance Substances 0.000 description 7
- 244000141359 Malus pumila Species 0.000 description 6
- 239000012773 agricultural material Substances 0.000 description 6
- 241000607479 Yersinia pestis Species 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 5
- 244000144730 Amygdalus persica Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 239000005871 repellent Substances 0.000 description 3
- 230000002940 repellent Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- XMGQYMWWDOXHJM-JTQLQIEISA-N (+)-α-limonene Chemical compound CC(=C)[C@@H]1CCC(C)=CC1 XMGQYMWWDOXHJM-JTQLQIEISA-N 0.000 description 2
- 241000675108 Citrus tangerina Species 0.000 description 2
- 241001133184 Colletotrichum agaves Species 0.000 description 2
- 240000000111 Saccharum officinarum Species 0.000 description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000002420 orchard Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241000188153 Isaria fumosorosea Species 0.000 description 1
- 241000168223 Isaria javanica Species 0.000 description 1
- 235000004347 Perilla Nutrition 0.000 description 1
- 244000124853 Perilla frutescens Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000002777 anti-larval effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- WPOOICLZIIBUBM-UHFFFAOYSA-H iron;iron(3+);methyl-dioxido-oxo-$l^{5}-arsane Chemical compound [Fe].[Fe+3].[Fe+3].C[As]([O-])([O-])=O.C[As]([O-])([O-])=O.C[As]([O-])([O-])=O WPOOICLZIIBUBM-UHFFFAOYSA-H 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000009406 nutrient management Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Abstract
본 발명은 친환경 과수 살균제 조성물의 제조방법에 관한 것으로, 더욱 상세하게는 우유, 소금 및 설탕을 혼합하여 혼합물을 제조하는 원료혼합단계 및 상기 원료혼합단계를 통해 제조된 혼합물을 발효하는 발효단계로 이루어진다.
상기의 과정을 통해 제조되는 친환경 과수 살균제 조성물은 천연성분으로 이루어져 친환경적이며, 사과, 배, 복숭아, 자두 및 매실나무 등에서 발생하는 화상병, 부란병, 가지마름병 및 수지병을 유발하는 병해에 대해 우수한 살균효과를 나타낸다.The present invention relates to a method for producing an eco-friendly fruit disinfectant composition. More specifically, it consists of a raw material mixing step of mixing milk, salt, and sugar to prepare a mixture, and a fermentation step of fermenting the mixture prepared through the raw material mixing step. .
The eco-friendly fruit disinfectant composition manufactured through the above process is made of natural ingredients, is eco-friendly, and has excellent sterilization against diseases that cause burns, rotting diseases, branch blight, and resin diseases occurring in apple, pear, peach, plum, and plum trees. Shows effect.
Description
본 발명은 친환경 과수 살균제 조성물의 제조방법에 관한 것으로, 더욱 상세하게는 천연성분으로 이루어져 친환경적이며, 사과, 배, 복숭아, 자두 및 매실나무 등에서 발생하는 화상병, 부란병, 가지마름병 및 수지병을 유발하는 병해에 대해 우수한 살균효과를 나타내는 친환경 과수 살균제 조성물의 제조방법에 관한 것이다.The present invention relates to a method of manufacturing an eco-friendly fruit tree disinfectant composition, and more specifically, is made of natural ingredients, is environmentally friendly, and causes burns, rotting diseases, branch blight and resin diseases that occur in apple, pear, peach, plum and plum trees, etc. It relates to a method of manufacturing an eco-friendly fruit tree disinfectant composition that exhibits excellent sterilizing effect against diseases and diseases.
병해의 퇴치를 위해 종래에는 주로 화학성분으로 이루어진 병해 퇴치제를 사용하여 왔으나, 화학성분으로 이루어진 병해 퇴치제는 토양의 산성화 및 오염문제, 인축에의 독성문제 및 농약성분의 잔류로 인한 환경문제 등을 유발한다.To eradicate diseases, pest repellents mainly composed of chemical ingredients have been used in the past. However, pest repellents made of chemical ingredients cause acidification and contamination of the soil, toxicity to humans and livestock, and environmental problems due to residual pesticide ingredients. do.
최근에는 환경문제에 대한 인식 제고와 소비자들의 친환경식품 선호현상으로 인해 화학농약을 대체할 수 있는 친환경농자제에 대한 관심이 커지고 있으며 그 사용도 점차 확대되고 있다.Recently, due to increased awareness of environmental issues and consumers' preference for eco-friendly foods, interest in eco-friendly agricultural pesticides that can replace chemical pesticides is growing, and their use is gradually expanding.
화학성분으로 이루어진 병해 퇴치제를 대체할 수 있는 친환경농자재로는, 식물에서 추출한 천연물 살균제의 사용 및 천적을 활용한 생물적 방제 인자의 사용이 있다. 이중 천연추출물을 이용한 나방 방제의 경우 대부분 효과가 객관적으로 검증되지 않은, 농민 개개인의 민간요법 차원인 경우가 많았으며, 또한, 몇몇 제조회사에 의해 상용화가 된 제품들이 있지만 방제효과가 미미하며, 제조방법의 까다로울 뿐만 아니라, 제한된 유통기간 등의 문제로 인해 화학성분으로 이루어진 병해 퇴치제를 대체하여 상용화되기에는 한계점이 있었다.Eco-friendly agricultural materials that can replace pest control agents made of chemical ingredients include the use of natural disinfectants extracted from plants and the use of biological control factors using natural enemies. Among these, in the case of moth control using natural extracts, the effectiveness was not objectively verified and was often a folk remedy for individual farmers. In addition, there are products commercialized by several manufacturers, but the control effect is minimal and the manufacturer Not only was the method difficult, but there were also limitations in commercializing it as a replacement for pest control agents made of chemical ingredients due to problems such as limited distribution period.
이러한 친환경농자재로서, 살균효과가 있는 천연 식물성분인 자소 잎 추출물을 이용하는 시도는 있었지만, 대부분 과거부터 살균효과가 있다고 알려져 있는 다른 식물과 혼합하여 사용하는 형태로서, 그 효과가 불분명하여 실질적으로 화학 방제제를 대체하여 사용하거나 방제제로 사용할 수 없는 실정이었다.As such an eco-friendly agricultural material, there have been attempts to use perilla leaf extract, a natural plant ingredient with a sterilizing effect, but most of them are used in combination with other plants that have been known to have a sterilizing effect in the past, and the effect is unclear, so chemical control is practically impossible. The situation was that it could not be used as a replacement for the agent or as a pest control agent.
한편, 친환경농자재란 인축 및 자연에 무해하면서 농작물에 양분 공급, 병해 억제, 토량개량 및 식물 생육 촉진에 사용되는 천연물질로된 환경 친화적 물질을 뜻하며, 친환경농자재는 현재 병해예방, 비료효과, 생육촉진 및 토양관리의 4개의 종류로 분류되고 있다.Meanwhile, eco-friendly agricultural materials refer to environmentally friendly materials made of natural materials that are harmless to humans and nature and are used to supply nutrients to crops, suppress diseases, improve soil quality, and promote plant growth. Eco-friendly agricultural materials are currently used to prevent diseases, have a fertilizer effect, and promote growth. and soil management are classified into four types.
친환경 농자재는 일반 농자재와는 달리 친환경농자재는 제품의 종류와 대상 물질이 매우 광범위하고, 이로 인해 각 제품이 가지는 특성과 유효성분이 서로 다르고 불확실한 경우가 많다. 친환경농업은 합성화학 비료나 농약사용을 최소화하면서 작물양분종합관리, 병해종합관리, 천적이용 등 첨단농업기술을 이용해 농업환경을 지속적으로 보전하는 모든 형태의 농업을 말하며, 과거 녹색혁명에 의한 주곡자급, 백색혁명으로 일컬어지는 비닐하우스시설원예 등은 효율적 농약 비료 사용 및 병해, 잡초 방제로 인해 수량증대는 물론 농산물의 품질향상에 크게 기여했다.Unlike general agricultural materials, eco-friendly agricultural materials have a very wide range of product types and target substances, and because of this, the characteristics and effective ingredients of each product are often different and uncertain. Eco-friendly agriculture refers to all forms of agriculture that continuously preserve the agricultural environment using cutting-edge agricultural technologies such as comprehensive crop nutrient management, comprehensive disease management, and use of natural enemies while minimizing the use of synthetic chemical fertilizers or pesticides. In the past, self-sufficiency in grains was achieved through the Green Revolution. , Gardening in greenhouses, also known as the White Revolution, has contributed greatly to not only increasing yield but also improving the quality of agricultural products due to efficient use of pesticides and fertilizers and control of diseases and weeds.
본 발명의 목적은 천연성분으로 이루어져 친환경적이며, 사과, 배, 복숭아, 자두 및 매실나무 등에서 발생하는 화상병, 부란병, 가지마름병 및 수지병을 유발하는 병해에 대해 우수한 살균효과를 나타내는 친환경 과수 살균제 조성물의 제조방법을 제공하는 것이다.The object of the present invention is to provide an eco-friendly fruit tree disinfectant composition made of natural ingredients and showing excellent sterilizing effects against diseases causing burns, rotting diseases, branch blights and resin diseases occurring in apple, pear, peach, plum and plum trees, etc. To provide a manufacturing method.
본 발명의 목적은 우유, 소금 및 설탕을 혼합하여 혼합물을 제조하는 원료혼합단계 및 상기 원료혼합단계를 통해 제조된 혼합물을 발효하는 발효단계로 이루어지는 것을 특징으로 하는 친환경 과수 살균제 조성물의 제조방법을 제공함에 의해 달성된다.The object of the present invention is to provide a method for producing an eco-friendly fruit tree disinfectant composition, characterized in that it consists of a raw material mixing step of mixing milk, salt and sugar to prepare a mixture, and a fermentation step of fermenting the mixture prepared through the raw material mixing step. is achieved by
본 발명의 바람직한 특징에 따르면, 상기 원료혼합단계는 우유 100 중량부, 소금 0.5 내지 1.5 중량부 및 설탕 0.5 내지 1.5 중량부를 혼합하여 이루어지는 것으로 한다.According to a preferred feature of the present invention, the raw material mixing step is performed by mixing 100 parts by weight of milk, 0.5 to 1.5 parts by weight of salt, and 0.5 to 1.5 parts by weight of sugar.
본 발명의 더 바람직한 특징에 따르면, 상기 발효단계는 2 내지 5℃의 온도에서 140 내지 160일 동안 이루어지는 것으로 한다.According to a more preferred feature of the present invention, the fermentation step is carried out at a temperature of 2 to 5 ° C. for 140 to 160 days.
본 발명의 더욱 바람직한 특징에 따르면, 상기 원료혼합단계에서는 상기 우유 100 중량부 대비 침엽수 추출물 1 내지 5 중량부가 더 함유되는 것으로 한다.According to a more preferred feature of the present invention, in the raw material mixing step, 1 to 5 parts by weight of coniferous extract is added based on 100 parts by weight of the milk.
본 발명의 더욱 더 바람직한 특징에 따르면, 상기 침엽수는 잣나무, 소나무 및 편백나무로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것으로 한다.According to an even more preferred feature of the present invention, the coniferous tree is made of one or more selected from the group consisting of pine tree, pine tree, and cypress tree.
본 발명의 더욱 더 바람직한 특징에 따르면, 상기 발효단계 이후에는 상기 발효단계를 통해 제조된 발효물 100 중량부 대비 감귤추출물 3 내지 5 중량부 및 사탕수수추출물 3 내지 5 중량부를 혼합하는 첨가제투입단계가 더 진행되는 것으로 한다.According to an even more preferred feature of the present invention, after the fermentation step, there is an additive input step of mixing 3 to 5 parts by weight of citrus extract and 3 to 5 parts by weight of sugarcane extract with respect to 100 parts by weight of the fermented product prepared through the fermentation step. Let’s proceed further.
본 발명의 더욱 더 바람직한 특징에 따르면, 상기 발효단계 이후에는 상기 발효단계를 통해 제조된 발효물에 세균에 대한 살균활성을 갖고 포자를 형성하는 곰팡이를 접종하고 배양하는 곰팡이접종단계가 더 진행되는 것으로 한다.According to an even more preferred feature of the present invention, after the fermentation step, a fungal inoculation step is further performed in which a mold that has bactericidal activity against bacteria and forms spores is inoculated and cultured in the fermented product produced through the fermentation step. do.
본 발명의 더욱 더 바람직한 특징에 따르면, 상기 친환경 과수 살균제 조성물은 화상병, 부란병, 가지마름병 및 수지병으로 이루어진 그룹에서 선택된 하나 이상에 적용되는 것으로 한다.According to an even more preferred feature of the present invention, the eco-friendly fruit tree disinfectant composition is applied to one or more selected from the group consisting of burn disease, rotting disease, branch dieback, and resin disease.
본 발명에 따른 친환경 과수 살균제 조성물의 제조방법은 천연성분으로 이루어져 친환경적이며, 사과, 배, 복숭아, 자두 및 매실나무 등에서 발생하는 화상병, 부란병, 가지마름병 및 수지병을 유발하는 병해에 대해 우수한 살균 효과를 나타내는 친환경 과수 살균제 조성물을 제공하는 탁월한 효과를 나타낸다.The manufacturing method of the eco-friendly fruit tree disinfectant composition according to the present invention is eco-friendly as it is made of natural ingredients, and has excellent sterilization against diseases causing burns, rotting disease, branch blight, and resin disease occurring in apple, pear, peach, plum, and plum trees. It shows excellent effectiveness in providing an eco-friendly fruit tree disinfectant composition that is effective.
도 1은 본 발명의 일 실시예에 따른 친환경 과수 살균제 조성물의 제조방법을 나타낸 순서도이다.
도 2는 본 발명의 다른 실시예에 따른 친환경 과수 살균제 조성물의 제조방법을 나타낸 순서도이다.
도 3은 본 발명의 또 다른 실시예에 따른 친환경 과수 살균제 조성물의 제조방법을 나타낸 순서도이다.Figure 1 is a flowchart showing a method of manufacturing an eco-friendly fruit tree disinfectant composition according to an embodiment of the present invention.
Figure 2 is a flowchart showing a method of manufacturing an eco-friendly fruit tree disinfectant composition according to another embodiment of the present invention.
Figure 3 is a flowchart showing a manufacturing method of an eco-friendly fruit tree disinfectant composition according to another embodiment of the present invention.
이하에는, 본 발명의 바람직한 실시예와 각 성분의 물성을 상세하게 설명하되, 이는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 발명을 용이하게 실시할 수 있을 정도로 상세하게 설명하기 위한 것이지, 이로 인해 본 발명의 기술적인 사상 및 범주가 한정되는 것을 의미하지는 않는다.Below, preferred embodiments of the present invention and the physical properties of each component are described in detail, but are intended to be described in detail so that those skilled in the art can easily practice the invention. This does not mean that the technical idea and scope of the present invention are limited.
본 발명에 따른 친환경 과수 살균제 조성물의 제조방법은 우유, 소금 및 설탕을 혼합하여 혼합물을 제조하는 원료혼합단계(S101) 및 상기 원료혼합단계(S101)를 통해 제조된 혼합물을 발효하는 발효단계(S103)로 이루어진다.The method for producing an eco-friendly orchard disinfectant composition according to the present invention includes a raw material mixing step (S101) of mixing milk, salt, and sugar to prepare a mixture, and a fermentation step (S103) of fermenting the mixture prepared through the raw material mixing step (S101). ).
이때, 상기 과수 살균제 조성물은 과수에서 발생하는 화상병, 부란병, 가지마름병 및 수지병으로 이루어진 그룹에서 선택된 하나 이상의 병해에 적용된다.At this time, the fruit tree disinfectant composition is applied to one or more diseases occurring in fruit trees selected from the group consisting of burn disease, rot disease, branch dieback, and resin disease.
상기 원료혼합단계(S101)는 우유, 소금 및 설탕을 혼합하여 혼합물을 제조하는 단계로, 우유 100 중량부, 소금 0.5 내지 1.5 중량부 및 설탕 0.5 내지 1.5 중량부를 혼합하여 이루어지는 것이 바람직하다.The raw material mixing step (S101) is a step of preparing a mixture by mixing milk, salt, and sugar. It is preferably performed by mixing 100 parts by weight of milk, 0.5 to 1.5 parts by weight of salt, and 0.5 to 1.5 parts by weight of sugar.
상기 우유는 그 종류가 특별히 한정되지 않고, 발효과정에서 초산(Acetic acid) 성분을 생성시키는 우유성분이라면 어떠한 것이든 사용가능하다.The type of milk is not particularly limited, and any milk component that generates acetic acid during the fermentation process can be used.
상기 소금은 0.5 내지 1.5 중량부가 함유되는데 과수 내에 침투한 병해를 유발하는 균을 살균시키는 과정을 통해 병해의 퇴치효과를 부여하는 역할을 한다.The salt contains 0.5 to 1.5 parts by weight and serves to provide a disease-fighting effect through the process of sterilizing disease-causing bacteria that have penetrated into the fruit tree.
이때, 상기 소금의 함량이 0.5 중량부 미만이면 상기의 효과가 미미하며, 상기 소금의 함량이 1.5 중량부를 초과하게 되면, 본 발명을 통해 제조되는 친환경 과수 살균제 조성물의 염도가 지나치게 높에 과수에 악영향을 미칠 수 있을 뿐만 아니라, 본 발명에 따른 친환경 과수 살균제 조성물을 제조하는 과정에서 진행되는 발효 중 미생물의 증식을 방해하여 발효 효율성을 저하시킬 수 있다.At this time, if the salt content is less than 0.5 parts by weight, the above effect is minimal, and if the salt content exceeds 1.5 parts by weight, the salinity of the eco-friendly fruit tree disinfectant composition prepared through the present invention is too high, adversely affecting the fruit trees. In addition, it may interfere with the growth of microorganisms during fermentation in the process of manufacturing the eco-friendly fruit disinfectant composition according to the present invention, thereby reducing fermentation efficiency.
또한, 상기 설탕은 0.5 내지 1.5 중량부가 함유되는데, 세균을 퇴치할 수 있는 아세트산 성분이 생성되도록 발효과정을 진행하는 과정에서 우유성분의 혐기성 발효가 진행되지 않도록 억제하는 역할을 한다.In addition, the sugar is contained in an amount of 0.5 to 1.5 parts by weight, and plays a role in preventing anaerobic fermentation of milk components from proceeding during the fermentation process to generate acetic acid that can combat bacteria.
이때, 상기 설탕의 함량이 0.5 중량부 미만이면 상기의 효과가 미미하며, 상기 설탕의 함량이 1.5 중량부를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 병해 살균제의 당성분이 지나치게 증가하여 세균의 먹이로 작용하는 부작용이 발생할 수 있다.At this time, if the sugar content is less than 0.5 parts by weight, the effect is minimal, and if the sugar content exceeds 1.5 parts by weight, the effect is not significantly improved, and the sugar component of the disease disinfectant increases excessively, increasing the number of bacteria. Side effects may occur as a result of feeding.
또한, 상기 원료혼합단계(S101)에서는 상기 우유 100 중량부 대비 침엽수 추출물 1 내지 5 중량부가 더 함유될 수도 있는데, 상기와 같이 침엽수 추출물이 더 함유되면, 본 발명에 따른 친환경 과수 살균제 조성물의 병해에 대한 퇴치효과가 더욱 향상될 수 있는데, 상기 침엽수 추출물은 정제수 100 중량부에 침엽수 20 내지 30 중량부를 혼합하고 80 내지 100℃의 온도에서 10 내지 30분 동안 가열한 후에 고형분을 제거하는 과정으로 이루어지는 것이 바람직하다.In addition, in the raw material mixing step (S101), 1 to 5 parts by weight of coniferous extract may be added compared to 100 parts by weight of milk. If more coniferous extract is contained as described above, it can prevent diseases of the eco-friendly fruit tree disinfectant composition according to the present invention. The repellent effect can be further improved, and the coniferous extract is made by mixing 20 to 30 parts by weight of coniferous trees with 100 parts by weight of purified water, heating at a temperature of 80 to 100 ° C. for 10 to 30 minutes, and then removing the solid content. desirable.
이때, 상기 침엽수는 잣나무, 소나무 및 편백나무로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것이 바람직한데, 상기에 기재된 침엽수에는 피톤치드 성분이 다량 함유되어 우수한 병해 퇴치효과를 나타낸다.At this time, the coniferous tree is preferably made of at least one selected from the group consisting of pine tree, pine tree, and cypress tree. The coniferous tree described above contains a large amount of phytoncide component and exhibits excellent disease fighting effect.
상기 발효단계(S103)는 상기 원료혼합단계(S101)를 통해 제조된 혼합물을 발효하는 단계로, 상기 원료혼합단계(S101)를 통해 제조된 혼합물을 2 내지 5℃의 온도에서 140 내지 160일 동안 발효하는 과정으로 이루어지는데, 상기의 과정을 통해 우유의 발효가 진행되면서 세균퇴치에 매우 효과적인 아세트산 성분이 다량 생성될 수 있다.The fermentation step (S103) is a step of fermenting the mixture prepared through the raw material mixing step (S101), and the mixture prepared through the raw material mixing step (S101) is fermented at a temperature of 2 to 5 ° C. for 140 to 160 days. It consists of a fermentation process, and as the fermentation of milk progresses through the above process, a large amount of acetic acid, which is very effective in eradicating bacteria, can be produced.
이때, 상기 발효온도가 2℃ 미만이거나 발효시간이 140일 미만인 경우에는 상기 원료혼합단계(S101)를 통해 제조된 혼합물의 발효가 제대로 진행되지 못하며, 상기 발효온도가 5℃를 초과하거나 발효시간이 160일을 초과하게 되면 발효효과는 크게 향상되지 않으면서 혐기성 발효가 진행될 수 있기 때문에 바람지하지 못하다.At this time, if the fermentation temperature is less than 2℃ or the fermentation time is less than 140 days, fermentation of the mixture prepared through the raw material mixing step (S101) does not proceed properly, and the fermentation temperature exceeds 5℃ or the fermentation time is less than 140 days. If it exceeds 160 days, it is undesirable because anaerobic fermentation may proceed without significantly improving the fermentation effect.
또한, 상기 발효단계(S103) 이후에는 상기 발효단계(S103)를 통해 제조된 발효물 100 중량부 대비 감귤추출물 3 내지 5 중량부 및 사탕수수추출물 3 내지 5 중량부를 혼합하는 첨가제투입단계(S105)가 더 진행될 수도 있다.In addition, after the fermentation step (S103), an additive input step (S105) of mixing 3 to 5 parts by weight of citrus extract and 3 to 5 parts by weight of sugarcane extract with respect to 100 parts by weight of the fermented product prepared through the fermentation step (S103). It may progress further.
상기 감귤추출물은 감귤을 지름이 10 내지 30 밀리미터를 나타내도록 분쇄한 후에, 분쇄된 감귤 100 중량부에 정제수 1000 내지 2000 중량부를 혼합하고 70 내지 80℃의 온도로 3 내지 5시간 동안 추출한 후에 고형분을 걸러내는 과정을 통해 제조된다.The citrus extract is made by crushing citrus fruits to have a diameter of 10 to 30 millimeters, mixing 1000 parts by weight of purified water with 100 parts by weight of pulverized tangerines, extracting the tangerines at a temperature of 70 to 80° C. for 3 to 5 hours, and then extracting the solid content. It is manufactured through a filtering process.
상기의 과정을 통해 제조되는 감귤추출물은 항균효과 및 항바이러스 또는 세균에 대한 소독 효과가 있으며, 산성을 띄고 있으며 다량 함유된 d-리모넨은 항균, 항바이러스, 항진균, 항유충 및 방취 등의 효과를 나타내는데, 상기 감귤추출물의 함량이 3 중량부 미만이면 상기의 효과가 미미하며, 상기 감귤추출물의 함량이 5 중량부를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 제조비용을 지나치게 증가시키게 된다.The citrus extract produced through the above process has antibacterial, antiviral, or disinfectant effects against bacteria, and d-limonene, which is acidic and contained in large amounts, has antibacterial, antiviral, antifungal, antilarval, and deodorizing effects. If the content of the citrus extract is less than 3 parts by weight, the effect is minimal, and if the content of the citrus extract exceeds 5 parts by weight, the effect is not significantly improved and the manufacturing cost is excessively increased.
또한, 상기 사탕수수추출물은 사탕수수를 지름이 10 내지 30 밀리미터를 나타내도록 분쇄한 후에, 분쇄된 사탕수수 100 중량부에 정제수 1000 내지 2000 중량부를 혼합하고 70 내지 80℃의 온도로 3 내지 5시간 동안 추출한 후에 고형분을 걸러내는 과정을 통해 제조된다.In addition, the sugarcane extract is prepared by crushing sugarcane to a diameter of 10 to 30 millimeters, mixing 1000 to 2000 parts by weight of purified water with 100 parts by weight of pulverized sugarcane, and stirring at a temperature of 70 to 80° C. for 3 to 5 hours. It is manufactured through a process of filtering out the solid content after extraction.
상기의 과정을 통해 제조되는 사탕수수추출물은 젖산이 다량 함유되어 있어 세균에 대한 살균효과를 나타내는데, 상기 사탕수수추출물의 함량이 3 중량부 미만이면 상기의 효과가 미미하며, 상기 사탕수수추출물의 함량이 5 중량부를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 제조비용을 지나치게 증가시키게 된다.The sugarcane extract prepared through the above process contains a large amount of lactic acid and has a sterilizing effect on bacteria. If the content of the sugarcane extract is less than 3 parts by weight, the effect is minimal, and the content of the sugarcane extract If the amount exceeds 5 parts by weight, the above effects are not significantly improved and the manufacturing cost is excessively increased.
또한, 상기 발효단계(S103) 이후에는 상기 발효단계(S103)를 통해 제조된 발효물에 세균에 대한 살균활성을 갖고 포자를 형성하는 곰팡이를 접종하고 배양하는 곰팡이접종단계(S105-11)가 더 진행될 수도 있는데, 상기 발효단계(S103)를 통해 제조된 발효물에 접종되는 세균에 대한 살균활성을 갖고 포자를 형성하는 곰팡이의 접종량은 특별히 한정되지 않지만, 곰팡이의 배양속도롤 고려했을 때, 1 내지 10×108 cfu/g이상으로 접종하는 것이 바람직하다.In addition, after the fermentation step (S103), a mold inoculation step (S105-11) is performed in which a mold that has bactericidal activity against bacteria and forms spores is inoculated and cultured in the fermented product prepared through the fermentation step (S103). The inoculation amount of the mold that has bactericidal activity and forms spores against the bacteria inoculated into the fermented product prepared through the fermentation step (S103) is not particularly limited, but considering the culture rate of the mold, it is 1 to 1. It is desirable to inoculate at 10×10 8 cfu/g or more.
상기 발효단계(S103)를 통해 발효된 발효물에 함유된 우유의 발효물은 상기 곰팡에가 배양될 수 있는 배지로 매우 적합하기 때문에, 상기와 같은 양으로 곰팡이가 접종되면 곰팡이가 빠르게 배양되어 세균에 대한 살균활성이 더욱 향상된 살균제 조성물을 제공할 수 있다.Since the fermented product of milk contained in the fermented product fermented through the fermentation step (S103) is very suitable as a medium in which the mold can be cultured, when the mold is inoculated in the same amount as above, the mold is quickly cultured and the bacteria It is possible to provide a disinfectant composition with further improved sterilizing activity.
이때, 상기 접종된 곰팡이의 배양조건은 그 종류에 따라 가변적이기는 하나, 25 내지 30℃의 온도에서 4 내지 7일 동안 이루어지는 것이 바람직하며, 상기 곰팡이의 종류는 세균에 대해 항균활성을 갖는 것이면 특별히 한정되지 않고 어떠한 것이든 사용가능하나, 이사리아 자바니카(Isaria javanica) Pf04 및 이사리아 푸모소로세(Isaria fumosorosea) 등을 사용할 수 있다.At this time, the culture conditions of the inoculated mold vary depending on the type, but are preferably carried out at a temperature of 25 to 30°C for 4 to 7 days, and the type of mold is specifically limited if it has antibacterial activity against bacteria. Any product can be used, but Isaria javanica Pf04 and Isaria fumosorosea can be used.
이하에서는, 본 발명에 따른 친환경 과수 살균제 조성물의 제조방법 및 그 제조방법으로 제조된 친환경 과수 살균제 조성물의 물성을 실시예를 들어 설명하기로 한다.Hereinafter, the manufacturing method of the eco-friendly fruit tree disinfectant composition according to the present invention and the physical properties of the eco-friendly fruit tree disinfectant composition prepared by the manufacturing method will be described using examples.
<제조예 1> 침엽수 추출물의 제조<Preparation Example 1> Preparation of coniferous extract
정제수 100 중량부에 편백나무 분쇄물 25 중량부를 혼합하고 90℃의 온도에서 20분 동안 가열한 후에 고형분을 걸러내어 제거하는 과정을 통해 침엽수 추출물을 제조하였다.A coniferous extract was prepared by mixing 25 parts by weight of cypress pulverized material with 100 parts by weight of purified water, heating at 90° C. for 20 minutes, and filtering out the solids.
<실시예 1><Example 1>
우유 1L에 소금 10g 및 설탕 10g을 혼합하고 3.5℃의 온도에서 150일 동안 발효하여 친환경 과수 살균제 조성물을 제조하였다.An eco-friendly fruit disinfectant composition was prepared by mixing 10 g of salt and 10 g of sugar in 1 L of milk and fermenting for 150 days at a temperature of 3.5°C.
<실시예 2><Example 2>
상기 실시예 1과 동일하게 진행하되, 우유 1L 대비 상기 제조예 1을 통해 제조된 침엽수 추출물 30g을 더 혼합하여 친환경 과수 살균제 조성물을 제조하였다.An eco-friendly fruit disinfectant composition was prepared in the same manner as in Example 1, but by mixing 30 g of the coniferous extract prepared in Preparation Example 1 with 1 L of milk.
상기 실시예 1 내지 2를 통해 제조된 친환경 과수 살균제 조성물을 부란병이 발생한 사과나무 부도위 도포하고, 치유효과를 측정하여 아래 표 1에 나타내었다.The eco-friendly orchard fungicide composition prepared in Examples 1 and 2 was applied to the apple trees suffering from rot disease, and the healing effect was measured and shown in Table 1 below.
{단, 치유효과는 종래 부란병 치료제로 사용되는 네오아소진을 양성대조군으로 하고, 무처리를 음성대조군으로 하였다. 치유효과는 부란병이 발생한 사과나무의 회복률을 계산하여 나타내는 방법을 이용하였다.{However, for the healing effect, Neoasozin, which is conventionally used as a treatment for burrowing disease, was used as a positive control, and untreated was used as a negative control. The healing effect was expressed by calculating the recovery rate of apple trees with rot disease.
회복률=부란이 발생된 부분의 캘로스(callus) 수/부란이 발생된 부분×100}Recovery rate = number of callus in the area where the burrow occurred/area where the burrow occurred × 100}
<표 1><Table 1>
상기 표 1에 나타낸 것처럼, 본 발명의 실시예 1 내지 2를 통해 제조된 친환경 과수 살균제 조성물은 부란병에 대해 우수한 회복효과를 부여하는 것을 알 수 있으며, 실시예 2를 통해 제조된 살균제 조성물은 양성대조군인 네오아소진과 대등한 효과를 나타내는 것을 알 수 있다.As shown in Table 1, it can be seen that the eco-friendly fruit tree disinfectant composition prepared through Examples 1 and 2 of the present invention provides an excellent recovery effect against rotting disease, and the disinfectant composition prepared through Example 2 is a positive control group. It can be seen that it has an effect equivalent to that of NeoAsojin.
따라서, 본 발명에 따른 친환경 과수 살균제 조성물의 제조방법은 천연성분으로 이루어져 친환경적이며, 사과, 배, 복숭아, 자두 및 매실나무 등에서 발생하는 화상병, 부란병, 가지마름병 및 수지병을 유발하는 병해에 대해 우수한 살균효과를 나타내는 친환경 과수 살균제 조성물을 제공한다.Therefore, the manufacturing method of the eco-friendly fruit tree disinfectant composition according to the present invention is environmentally friendly as it is made of natural ingredients, and is effective against diseases causing burns, rotting diseases, branch blight, and resin diseases occurring in apple, pear, peach, plum and plum trees, etc. An eco-friendly fruit tree disinfectant composition that exhibits excellent sterilization effect is provided.
S101 ; 원료혼합단계
S103 ; 발효단계
S105 ; 첨가제투입단계
S105-1 ; 곰팡이접종단계S101 ; Raw material mixing stage
S103 ; Fermentation stage
S105 ; Additive input stage
S105-1 ; Fungal inoculation stage
Claims (8)
상기 원료혼합단계를 통해 제조된 혼합물을 발효하는 발효단계;로 이루어지며,
상기 원료혼합단계는 우유 100 중량부, 소금 0.5 내지 1.5 중량부 및 설탕 0.5 내지 1.5 중량부를 혼합하여 이루어지고,
상기 발효단계는 2 내지 5℃의 온도에서 140 내지 160일 동안 이루어지는 것을 특징으로 하는 친환경 과수 살균제 조성물의 제조방법.
A raw material mixing step of mixing milk, salt, and sugar to produce a mixture; and
It consists of a fermentation step of fermenting the mixture prepared through the raw material mixing step,
The raw material mixing step is performed by mixing 100 parts by weight of milk, 0.5 to 1.5 parts by weight of salt, and 0.5 to 1.5 parts by weight of sugar,
A method for producing an eco-friendly fruit tree disinfectant composition, characterized in that the fermentation step is carried out for 140 to 160 days at a temperature of 2 to 5 ° C.
상기 원료혼합단계에서는 상기 우유 100 중량부 대비 침엽수 추출물 1 내지 5 중량부가 더 함유되는 것을 특징으로 하는 친환경 과수 살균제 조성물의 제조방법.
In claim 1,
A method for producing an eco-friendly fruit tree disinfectant composition, characterized in that in the raw material mixing step, 1 to 5 parts by weight of coniferous extract is added compared to 100 parts by weight of the milk.
상기 침엽수는 잣나무, 소나무 및 편백나무로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것을 특징으로 하는 친환경 과수 살균제 조성물의 제조방법.
In claim 4,
A method for producing an eco-friendly fruit tree disinfectant composition, characterized in that the coniferous tree consists of one or more selected from the group consisting of pine tree, pine tree and cypress tree.
상기 발효단계 이후에는 상기 발효단계를 통해 제조된 발효물 100 중량부 대비 감귤추출물 3 내지 5 중량부 및 사탕수수추출물 3 내지 5 중량부를 혼합하는 첨가제투입단계가 더 진행되는 것을 특징으로 하는 친환경 과수 살균제 조성물의 제조방법.
In claim 1,
After the fermentation step, an additive injection step of mixing 3 to 5 parts by weight of citrus extract and 3 to 5 parts by weight of sugar cane extract is further performed, based on 100 parts by weight of the fermented product prepared through the fermentation step. An eco-friendly fruit tree disinfectant. Method for producing composition.
상기 발효단계 이후에는 상기 발효단계를 통해 제조된 발효물에 세균에 대한 살균활성을 갖고 포자를 형성하는 곰팡이를 접종하고 배양하는 곰팡이접종단계가 더 진행되는 것을 특징으로 하는 친환경 과수 살균제 조성물의 제조방법.
In claim 1,
After the fermentation step, a fungal inoculation step is further performed in which a mold that has bactericidal activity against bacteria and forms spores is inoculated and cultured in the fermented product produced through the fermentation step. .
상기 친환경 과수 살균제 조성물은 화상병, 부란병, 가지마름병 및 수지병으로 이루어진 그룹에서 선택된 하나 이상에 적용되는 것을 특징으로 하는 친환경 과수 살균제 조성물의 제조방법.The method according to any one of claims 1 and 4 to 7,
A method for producing an eco-friendly fruit tree disinfectant composition, characterized in that the eco-friendly fruit tree disinfectant composition is applied to one or more selected from the group consisting of burn disease, rot disease, branch blight, and resin disease.
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