KR101878896B1 - Composition comprising microorganism fermentation liquid for malodor removal - Google Patents

Abstract

The present invention relates to a malodor removing composition comprising a microbial fermentation broth as an effective ingredient, a malodor removal method for treating the composition, and a method for producing a compost containing the composition. The composition according to the present invention enables environmentally friendly removal of odors, increases the efficiency of cultivation of crops, and increases the productivity of animal husbandry.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for removing odor containing a microbial fermentation broth,

TECHNICAL FIELD The present invention relates to a composition for removing bad odors containing a microbial fermentation broth, a method for removing odors using the composition, and a method for manufacturing a compost for treating the composition.

In 2015, domestic consumption of chicken was about 18 per person per year, showing steady demand. In the case of goats, goat breeding farms are about 20 534 farms as of 2015, In order to meet such demand, intensive and business livestock farming methods have been carried out.

However, due to the increase in national income and the strengthening of environmental education, the paradigm of the livestock farming method has been changed, and the social interest in the production process of livestock products is also increasing. Particularly, there are new trends in the transition from group breeding to welfare rearing and the indiscriminate use of antibiotics. However, there are many difficulties to apply to the actual livestock industry considering the economic efficiency.

In the case of livestock breeding through the above-mentioned group breeding, there was found a problem that a considerable odor was generated in the breeding process due to intensive breeding, and in particular, there was a stinking odor in the meat and a case where the consumer was reluctant to eat meat .

On the other hand, livestock manure produced during livestock breeding is generally reproduced as compost. However, the odor generated when producing compost with livestock manure is a mixed odor mixed with ammonia, hydrogen sulfide, trimethylamine and methyl mercaptan. Generally, since the size of the compost is large, the amount of odor air to be treated is several thousand m 3 / do.

At present, as a method for removing odors generated in a composting facility using livestock manure, a method of chemically removing odors such as a chemical solution cleaning method is mainly used. However, when the chemical odor is chemically removed as described above, there is a problem that a secondary pollutant (waste water) is generated because a chemical is used.

In this regard, in Korean Patent Laid-Open Publication No. 2013-0071047, the odor removing method has been proposed through the drying device, but no method for preventing the smell of the compost itself has been suggested at all.

The inventors of the present invention have found that when the composition prepared by the present invention is sprayed, it has an effect of removing the odor generated in the domestic animal and the odor generated in the domestic animal manure, Thus completing the present invention.

Accordingly, it is an object of the present invention to provide a composition for removing odor containing a microbial fermentation broth.

However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.

In order to accomplish the object of the present invention as described above, the present invention provides a composition for removing bad odors comprising a microbial fermentation broth as an active ingredient.

In one embodiment of the present invention,

A step (step 1) of making charcoal for 10 to 12 days in an inactivated state of oak, pine, oak, paulownia, and chaff in which the thermophilic microorganism has been inactivated;

The oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal, chaff charcoal and chaff charcoal from which the thermophilic microorganism has been inoculated is taken out from the bulgum, and the air in the bulgur is pierced, For 36-48 hours (step 2);

A step (step 3) of mixing the mud left in the inhaler and inoculated with the thermophilic microorganism on each of the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal;

(Step 4) of covering the first rice straw on the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal mixed with the mud which is inoculated with the thermophilic microorganism and inactivated;

Mixing the fermented oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal, and chaff charcoal to prepare a fermented product (Step 5);

A step of mixing the loess and the second rice straw and aging the rice while supplying oxygen in the aeration tank (step 6); And

Mixing the fermented product with the fermented product and aging in an aeration tank (step 7).

In another embodiment of the present invention, the thermophilic microorganism is selected from the group consisting of Lactobacillus acidophilus , Bifidobacterium bifidum , Streptococcus thermophilus , and Bacillus farumus Bacillus firmus ). ≪ / RTI >

The present invention also relates to a microbial fermentation broth comprising a first microorganism preparation prepared by mixing lacquer, green tea leaves, and bamboo stalks, aged microorganism preparation, a second microorganism preparation prepared by mixing and aging ginkgo biloba, And a third microorganism preparation which is aged and mixed can be prepared.

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The present invention also relates to a method for producing a microorganism by mixing 1 to 5 parts by weight of a third rice straw with 100 parts by weight of a fourth microorganism preparation in which the first microorganism preparation, the second microorganism preparation and the third microorganism preparation are mixed at a weight ratio of 1: 1: 1 Followed by aging.

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The present invention relates to a composition for removing malodor comprising a microbial fermentation broth as an active ingredient. The composition can be applied directly to livestock or treated with animal manure to produce environmentally friendly compost. As a result of treating the composition of the present invention with chlorine, it was found that the intestine of the meat was removed, and when it was included in the manure of the livestock for the compost production, the odor of the compost was removed, It is confirmed that the composition can be usefully used as a composition for a liquid crystal display device.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photograph showing a photograph of a compost containing a composition for removing odor of the present invention. FIG.
FIG. 2 is a graph showing growth results of compost-grown strawberries and strawberries that do not contain a malodor removing composition containing the composition for removing odors of the present invention.
FIG. 3 is a diagram showing the growth results of strawberry cultivated as compost including the composition for removing odor of the present invention and strawberries not containing the composition for removing odor (left: no treatment, right: treatment).
FIG. 4 is a graph showing growth results of a strawberry cultivated as compost containing the composition for removing odor of the present invention and strawberries not containing the composition for removing odor (left: no treatment, right: treatment).

According to the present invention, there is an increasing demand for the production of environmentally friendly compost with improved odor as well as the production of high quality livestock products. Therefore, in studying a new method for removing bad odors, charcoal and clay inoculated with a thermophilic microorganism are mixed and a fermentation liquid is used , It was confirmed that the fermentation broth had an odor removing effect, and the present invention was completed.

That is, it is an object of the present invention to provide a composition for removing malodor comprising a microbial fermentation broth as an effective ingredient.

The microbial fermentation broth may contain,

A step (step 1) of making charcoal for 10 to 12 days in an inactivated state of oak, pine, oak, paulownia, and chaff in which the thermophilic microorganism has been inactivated;

The oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal, chaff charcoal and chaff charcoal from which the thermophilic microorganism has been inoculated is taken out from the bulgum, and the air in the bulgur is pierced, For 36-48 hours (step 2);

A step (step 3) of mixing the mud left in the inhaler and inoculated with the thermophilic microorganism on each of the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal;

(Step 4) of covering the first rice straw on the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal mixed with the mud which is inoculated with the thermophilic microorganism and inactivated;

Mixing the fermented oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal, and chaff charcoal to prepare a fermented product (Step 5);

A step of mixing the loess and the second rice straw and aging the rice while supplying oxygen in the aeration tank (step 6); And

Mixing the fermentation product with the fermentation product and aging in an aeration tank (step 7).

The thermophilic microorganism used in the present invention means a microorganism capable of living at a high temperature. The microorganism produces a special enzyme and various high-molecular substances and has high resistance to toxic substances such as high temperature environment, heavy metals and hydrogen sulfide. Wherein the thermophilic microorganism is preferably Lactobacillus know also loose fill (Lactobacillus acidophilus), Bifidobacterium bipyridinium bushes (Bifidobacterium bifidum , Streptococcus thermophilus , or Bacillus firmus may be used.

Hereinafter, the method for producing the fermentation broth contained in the composition of the present invention will be described in more detail by stages.

[Step 1]

In step 1, 0.1 to 1 part by weight of a thermophilic microorganism is preferably inoculated into 100 parts by weight of each of oak, pine, aspen, paulownia and rice husk. If less than 0.1 part by weight of the thermophilic microorganism is inoculated, there is a problem that the activity of the microorganism is reduced. If more than 1 part by weight of the microorganism is inoculated, the microorganism grows rapidly and stagnates.

The oak, pine, aspen and paulownia are preferably cut to 30 cm. The temperature at which the flame is turned off is 80 to 90 占 폚.

When the oak, pine, aspen, paulownia, and chaff are inactivated (80 ~ 90 ℃) for 10 ~ 12 days in which the thermophilic microorganism has been inactivated, it becomes a semi-charcoal. The reason why charcoal is kept at 80 ~ 90 ℃ without heating by high temperature is because the activity of thermophilic microorganisms is decreased when the temperature is too high.

The oak does not refer to any one species but refers to various species belonging to the oak and the oak. It means a useful tree because it has many uses, and all the trees belonging to this genus are called 'acorn trees' because they produce nuts called acorns. There are deciduous broad-leaved trees with fallen leaves in winter and evergreen broad-leaved trees that do not support leaves throughout the year. Most of them are arboreous trees over 8m in height and sometimes shrubs within 2m in height. It grows 200-250 species from temperate to tropical tropics in the Northern Hemisphere, with leaf stalking and sawtooth on most edges. Flowers are bisexual and bloom from April to May. The male flower eagle sits down from the axilla of the new branch, and the female flower eagle stands straight on the upper part of the leaf axilla. The nuts, called acorns, are contained in a plate-like shell (斗 斗) and are oval or ball-shaped. Each head is gunshot and is covered with foreign bracts. The porins vary in size and arrangement depending on the species. Because the bark has a lot of tannin content, it is used to dye fishing nets at the beach. Lumber is very hard and used in many places, especially famous for the materials that make keg. Nuts are picked and made with Dorotomi. The leaves of the oak tree are large, thick and fragrant, and have been used in rice fields for rice cakes, but in Japan, they are used to wrap rice cakes. Cork is picked from oak oak, especially in the temperate zone, cultivating Mediterranean cork oak (Q.suber) as an economic resource to produce cork.

The pine tree is an evergreen needlework tree of the ginseng plant pine tree and contains a large amount of chlorophyll, protein, crude fat, phosphorus, iron, enzyme, essential oil, minerals, vitamin A and vitamin C. The neck of the pine tree has terpene hydrate, pinosylvin, pinosylvin monomethyl ester, dihydropinosylvin monomethyl ester, pinosembrine, pinovan, propionaldehyde, serotinic acid, unperic acid, The oil of branches and joints contains carnipol, abietic acid, essential oil.

The ash is a member of the Oleeae family of Oleacae, and about 60 species are distributed mainly in the northern hemisphere, East Asia and North America. Several species of ash trees are known to be important for the formation of parks and the greening of settlements because they are durable, strong to impact and able to be used as timber that exhibits upright character and form an excellent shade . The ash tree (Fraxinus rhynchophylla Hance) is called Jinbacuki (秦 白木). It is used as a medicinal herb for the treatment of bacterial dyslipidemia, chronic bronchitis, cataract and glaucoma, and has a herb medicine such as aesculin, aesculetin and 6-β-d-glucoside, which are active as analgesics, anti-inflammatory agents, anti-inflammatory agents, astringents, astringents, diuretics, expectorants and dry digestive agents. They control bacterial infections and colds, , Diarrhea, dysentery, eye diseases such as cataracts, coughing, and asthma.

The Paulownia coreana Uyeki is a deciduous broad-leaved arboreous tree belonging to the genus Hornbeam. The height of the paulownia tree is about 15m, and the bark of the tree is pale brown, and the line of dark brown is vertical. It is known that the papaya peel is effective for bronchopneumonia, bacterial diarrhea, acute enteritis, acute conjunctivitis, etc. The papilled leaves are called the lobes, which are known to be effective for facial flushing and pustule.

The rice hull is a by-product produced in a large amount in the rice paddy processing step, and the amount of the rice hull is sufficient, and a constant amount is always generated during the year, so that it is not only stable but also cheap, .

[Step 2]

In step 2, 5 to 10 parts by weight of the thermophilic microorganism is preferably inoculated to 100 parts by weight of the mud. If less than 5 parts by weight of the thermophilic microorganism is inoculated, there is a problem that the number of microorganisms is reduced. When the microorganism is inoculated in an amount exceeding 10 parts by weight, there is a problem that it is in conflict with microorganisms in the existing mud.

The mud is not particularly limited, but it is preferable to use clay in a summer rice field which is excellent in the activity of Bacillus sp. And contains a large amount of amino acids and minerals.

The thermophilic microorganism may be the same as the thermophilic microorganism used in step 1 above.

[Step 3]

In the step 3, it is preferable that the mildew of the oak charcoal, the pine charcoal, the ash wood charcoal, the paulownia charcoal and the chaff charcoal is inoculated with the thermophilic microorganism and the inactivated mud is mixed at a weight ratio of 2: 1.

[Step 4]

The step 4 is to cover the first rice straw on the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal mixed with the mud which is inoculated with the thermophilic microorganism and allowed to stand at 30-50 ° C for 20-30 Lt; / RTI > days. When fermented at a temperature lower than 30 ° C., there is a problem that the growth is lowered. If the fermentation is carried out at a temperature higher than 50 ° C., the heat of the rice straw is exacerbated to excessively promote the growth of microorganisms, have.

If the fermentation is performed for less than 20 days, there is a problem that the microorganism is easily killed upon completion of culturing the microorganism. If the fermentation is performed for more than 30 days, the microorganism is automatically killed during cultivation.

[Step 5]

In step 5, it is preferable to mix the fermented oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal, and chaff charcoal in a weight ratio of 2: 1: 1: 1: 2 to prepare a fermented product. The reason for mixing at the above ratio is that the effect produced by the action of the bacterium for each tree species is different.

The clay used in the fermentation in step 4 removes the harmful microorganisms through the microbial activity and sinks down with the foreign substance.

[Step 6]

In the step 6, it is preferable to mix the yellow clay and the second rice straw at a weight ratio of 1:10, and aged at 20 to 35 ° C while supplying oxygen in the aeration tank for 7 to 10 days.

Horticulture has a tendency to inhibit the growth of microorganisms. However, if stuck with microorganisms, microorganisms can survive without losing the minerals in the loess, and they can also become familiar with the antimicrobial components of the loess.

When aged at a temperature of less than 20 ° C, microorganisms can not eat minerals of the loess, resulting in microorganisms dying. When aged at a temperature of more than 35 ° C, microorganisms easily ingest loess minerals, .

When aged for less than 7 days, the microorganisms do not take up the minerals of the loess, resulting in poor activity. When aged for more than 10 days, the microorganisms easily eat the minerals in the loess, have.

[Step 7]

The step 7 is a step of mixing 10 to 20 parts by weight of the fermented product with 100 parts by weight of the aged material and aging the mixture at a low temperature of 1 to 5 ° C for 60 to 70 days in an aeration tank to produce a microorganism fermentation broth.

If the fermented product is contained in an amount of less than 10 parts by weight based on 100 parts by weight of the fermented product, there is a problem that the bacteria are less contained and the malodor removing effect is impaired. If the fermented product is contained in an amount exceeding 20 parts by weight, have.

When aged at a temperature lower than 1 ° C, there is a problem that the microorganism is not activated. When aged at a temperature higher than 5 ° C, the microorganism is sourced at the time of aging to collect the mycelium at the bottom.

If aged for less than 60 days at the time of aging, there is a problem that beneficial bacteria that can remove odor are easily killed, and when aged for more than 70 days, they are searched, causing a problem of odor.

The present invention relates to a method for producing a microbial fermentation broth comprising mixing 0.1 to 1 part by weight of lacquer, 0.1 to 1 part by weight of green tea leaves and 0.1 to 1 part by weight of bamboo stem in 100 parts by weight of the microbial fermentation broth and matureting at 1 to 5 ° C for 60 to 70 days Can be added. By mixing the microbial fermentation broth with lacquer, green tea leaves and bamboo stalks and matureing at low temperature, the function of removing the smell of compost can be further improved. The above-mentioned lacquer stands for the youngest of the lacquer tree. The lacquer, green tea leaves and bamboo stalks are preferably dried and used.

The present invention relates to a method for producing a microbial fermentation broth comprising mixing 0.1 to 1 part by weight of Angelica gigas L., 0.1 to 1 part by weight of bivalvarial stem and 0.1 to 1 part by weight of ginkgo fruit in 100 parts by weight of the microorganism fermentation broth and matured at 1 to 5 ° C for 60 to 70 days Can be added. The function of preventing insects from composting can be further improved by mixing Angelica japonica, Angelica gigas Nakai, and Ginkgo biloba in the microbial fermentation broth and matureing them at low temperature.

In the present invention, 0.1 to 1 part by weight of Mulberry leaf, 0.1 to 1 part by weight of mulberry leaf and 0.1 to 1 part by weight of Staphylococcus aureus are mixed with 100 parts by weight of the above microbial fermentation broth, followed by low temperature aging at 1 to 5 ° C for 60 to 70 days can do. Mulberry leaf, mulberry leaf, and stalks are mixed with the microbial fermentation broth and matured at a low temperature to further improve the function of removing odor.

In the present invention, 0.1 to 1 part by weight of lacquer, 0.1 to 1 part by weight of green tea leaves and 0.1 to 1 part by weight of bamboo stem are mixed with 100 parts by weight of the microorganism fermentation broth and matured at 1 to 5 ° C for 60 to 70 days at low temperature, Microbiological preparation,

0.1 to 1 part by weight of Angelica keiskei koidz., 0.1 to 1 part by weight of mulberry stem and 0.1 to 1 part by weight of ginkgo fruit are mixed with 100 parts by weight of the microorganism fermentation broth and the mixture is aged at 1 to 5 ° C for 60 to 70 days to obtain a second microorganism preparation Create,

0.1 to 1 part by weight of Mulberry leaf, 0.1 to 1 part by weight of mulberry leaf and 0.1 to 1 part by weight of Staphylococcus aureus are mixed with 100 parts by weight of the microorganism fermentation broth, and the mixture is aged at 1 to 5 ° C for 60 to 70 days to obtain a third microorganism preparation,

1 to 5 parts by weight of a third rice straw is mixed with 100 parts by weight of a fourth microorganism preparation prepared by mixing the first microorganism preparation, the second microorganism preparation and the third microorganism preparation in a weight ratio of 1: 1: 1, RTI ID = 0.0 > 30 C < / RTI > for 40 days.

Since the third rice straw can use only the rice straw-specific fermenting bacteria at a subzero temperature, it is preferable to cut rice straw roots accumulated for 10 ~ 60 days under the eyes to 10 ~ 12 cm.

If less than 1 part by weight of the third rice straw is contained relative to 100 parts by weight of the fourth microorganism preparation, there is a problem that the specific fermenting bacteria of rice straw are insufficient. If the amount is more than 5 parts by weight, the fermenting bacteria unique to rice straw may excessively eat the bacteria there is a problem.

When aged at a temperature lower than 25 ° C, there is a problem of poor growth. When aged at a temperature higher than 30 ° C, heat of rice straw is exacerbated and microbial growth is promoted excessively.

When aged for less than 30 days, there is a problem that the growth is lowered. When aged for more than 40 days, the rice straw is exothermic and the microorganism grows excessively.

It is preferred that the microorganism preparation is allowed to stand for 10 days after turning off the aeration to take up supernatant.

In addition, the present invention can provide a malodor removing method comprising the step of treating the malodor removing composition.

There is no limitation on the method for removing odor, but it is preferable that the composition is sprayed in water.

The malodor removing method may be used directly for livestock or for manure of livestock. Examples of the livestock include chicken, goat, pig, cow, cow, horse, donkey, sheep, rabbit, cat, Goose, pheasant, quail, ostrich, mallard or turkey, and the like.

The present invention also provides a method for producing a compost comprising the composition for removing odor comprising the steps of:

Preparing a raw material for the compost (step 2-1); And

Treating the raw material with the composition for removing odor (Step 2-2).

The raw material of the compost in the step 2-1 means a raw material used in general compost production such as manure of livestock. Preferably, the raw material of the compost may be a chlorine manure as in the embodiment of the present invention. But is not limited to.

In the step 2-2, the composition for removing odor containing the microbial fermentation liquid prepared as described above is treated to the raw material. There is no limitation on the method of the treatment, and after the treatment in the step 2-2, the fermentation can be completed at a temperature of 45 to 70 占 폚 to complete the compost.

In the present invention, the composition prepared by the method described above was treated with livestock to show a good odor removing effect as compared with the untreated control group in the odor elimination sensory evaluation (Example 2). In addition, it was confirmed that the composition of the present invention was included in the manure of the livestock to produce compost, and the production of ammonia, hydrogen sulfide, trimethylamine and methyl mercaptan was remarkably reduced (Example 3). As a result of growing the crop using the compost, it was confirmed that the growth of the crop was increased (Example 4).

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

Example  1: Preparation of composition for removing odor of the present invention

Oak, pine, ash, paulownia and rice hulls were prepared and inoculated with Bacillus firmus and Bacillus subtilis as respiratory microorganisms.

The oak, pine, aspen, paulownia, and chaff were inoculated with fire-retardant microbes for 12 days to produce charcoal. 100 parts by weight of each of oak, pine, aspen, paulownia and rice husk were inoculated with 0.5 part by weight of a thermophilic microorganism. The oak, pine, aspen and paulownia were cut into 30 cm and used. The temperature at which the flame is turned off is 110 ° C. The oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal from which the thermophilic microorganism has been inoculated is taken out from the bulgum, For 24 hours. The thermophilic microorganism inoculated in the mud was the same kind of thermophilic microorganism as the thermophilic microorganism inoculated to the above tree. 100 parts by weight of the mud was inoculated with 5 to 10 parts by weight of a thermophilic microorganism. The mud used mud in summer rice fields. The above-mentioned thermophilic microorganisms were inoculated into each of the above-mentioned oak charcoal, pine charcoal, ash charcoal, paulownia charcoal, and chaff charcoal, and the mud left in the bulgogi was mixed at a weight ratio of 2: 1. The first rice straw was put on the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal, and chaff charcoal, which were inoculated with the above-mentioned thermophilic microorganisms and mixed with the mud left in the crucible, and fermented at 50 ° C for 30 days. The fermented product was prepared by mixing the fermented oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal in a weight ratio of 2: 1: 1: 1: 2. The yellow loess and the second rice straw were mixed at a weight ratio of 1:10, and aged at 20 캜 while supplying oxygen in the aeration tank for 7 days. To 100 parts by weight of the aged material, 15 parts by weight of the fermentation product was mixed and aged for 60 days in an aeration tank at a low temperature of 3 캜 to prepare a microorganism preparation. 0.3 parts by weight of lacquer, 0.3 part by weight of green tea leaves and 0.3 part by weight of bamboo stem were mixed with 100 parts by weight of the microorganism preparation, followed by low temperature aging at 3 캜 for 60 days to prepare a first microorganism preparation. To 100 parts by weight of the microorganism preparation, 0.3 part by weight of Angelica giganteus, 0.3 part by weight of bivalvia, and 0.3 part by weight of Ginkgo biloba were mixed and aged at 3 캜 for 60 days at a low temperature to prepare a second microorganism preparation. 0.3 part by weight of mugwort, 0.3 part by weight of mulberry leaf, and 0.3 part by weight of mature stem were mixed with 100 parts by weight of the microorganism preparation, and the mixture was aged at 3 캜 for 60 days at a low temperature to prepare a third microorganism preparation. 3 parts by weight of a third rice straw was mixed with 100 parts by weight of a fourth microorganism preparation in which the first microorganism preparation, the second microorganism preparation and the third microorganism preparation were mixed at a weight ratio of 1: 1: 1, After aging for one day, the aeration was turned off, and the mixture was allowed to stand for 10 days, and then the supernatant was taken to prepare a composition for deodorization of odor. The third rice straw was cut into 10 cm of rice straw roots accumulated for 30 days under the eyes.

Example  2: Treatment of composition for deodorization of odor in livestock

In this example, the composition prepared above was directly processed into livestock and livestock manure.

As a result, as shown in Table 1 below, pigs were sprayed with water (1000 tons) containing 10 to 15 kg of the composition for 24 hours to deodorize the offensive odor, and 30 kg (300 tons) was sprayed for 120 hours, and chlorine was sprayed with water (200 tons) containing 50 kg of the composition for deodorization.

As a result, when 10 to 15 kg of the composition was sprayed to pigs, the same deodorizing effect as that of 1000 tons of water was used, and when 30 kg of the composition was sprayed on the chicken, the same deodorizing effect as that of 300 tons of water was used. Was deodorized with the same deodorizing effect as using 200 tons of water.

division Application criteria time usage pig 1000 tons of water 24 hours (1 day) 10 ~ 15kg chicken 300 tons of water 120 hours (5 days) 30kg Goat 200 tons of water 240 hours (10 days) 50kg

Example  3: Confirmation of compost production and odor removal effect of compost

The deodorizing effect of the microbial fermentation broth prepared in Example 1 was evaluated through sensory evaluation. 15 parts by weight of the microbial fermentation broth prepared in Example 1 was mixed with 100 parts by weight of chlorine manure, and the mixture was allowed to stand for 24 hours (FIG. 1). The composition analysis results of the produced composts are shown in Table 2 (Analytical method: fertilizer quality test method, standard: Notification No. 2013-5 of Rural Development Administration).

Black item unit Process standard Test result Organic matter % 30 or more 36.94 Organic versus food consumption - 45 or less 27.77 Arsenic (As) mg / kg About the building 45 or less 0.61 Cadmium (Cd) mg / kg 5 or less 0.05 Mercury (Hg) mg / kg 2 or less 0.07 Lead (Pb) mg / kg 130 or less 0.00 Chromium (Cr) mg / kg 200 or less 10.30 Copper (Cu) mg / kg 45 or less 33.02 Nickel (Ni) mg / kg 900 or less 7.68 Zinc (Zn) mg / kg 1.8 or less 306.28 salt % Non-detection 1.37 E. coli O157: H7 - Non-detection Non-detection Salmonella - 55 or less Non-detection moisture % Completed or 70 or more 43.04 Compost - 25 or less Compartmentalized Hydrochloric acid insoluble seawater % 10.33

For comparison with the manufactured compost, 15 parts by weight of a commercially available microbial culture solution (natural wax, micro-max cultivation combination method) was mixed with 100 parts by weight of chlorine manure, and the mixture was allowed to stand for 24 hours. After 24 hours, 1 point was given for good odor removal, 3 points for normal odor removal, and 5 points for poor odor removal. A total of 10 samples were evaluated, and the results are shown in Table 3.

sample Control 1 Comparison group 1 Composition (% by weight) apply Unapplied One One 4 2 3 3 3 2 4 4 2 4 5 One 4 6 3 3 7 2 4 8 One 4 9 2 3 10 2 4 Sum (average) 19 37

As can be seen from the above Table 3, it can be confirmed that the composition for removing odor according to the present invention is superior in odor removal effect when compost is manufactured, compared with the microbial preparation of Comparative Example 1. [

In addition, the production amount of the odorous substance was evaluated using a FIS (Gas Chromatograph) model as a test instrument. The experimental conditions were as follows. The evaluation results are shown in Table 4.

1) Room temperature: 30 ℃

2) Relative humidity: 50%

3) Size of container (container for deodorization test): 50cm × 50cm × 60cm

4) Amount of sample: 10ml

sample Elapsed time Analysis (ppm)
ammonia Hydrogen sulfide Trimethylamine Methyl mercaptan Compost comprising the composition of the present invention 0 98 50 48 30 0.5 89 22 23 20 1.0 78 21 22 15 1.5 62 15 17 12 2.0 60 13 12 10 2.5 60 5 10 5 Compost containing commercially available microbial agents 0 98 50 50 30 0.5 95 45 47 29 1.0 94 44 43 27 1.5 92 42 40 25 2.0 90 40 39 23 2.5 89 39 37 22

As can be seen from the above Table 4, the compost containing the composition for removing odor according to the present invention was found to be superior in odor removal effect to the compost produced including commercially available microbial agents even in the evaluation using the deodorization test instrument have.

In order to reliably confirm the performance of the composition for removing odor of the present invention, an experiment was commissioned by the Korean Chemical Fusion Test Institute and the results shown in Table 5 were obtained. The experimental conditions are as follows.

1. Experimental environment: Temperature (25 ± 5) ℃, humidity (45 ± 5)% R.H.

2. Test vessel: 10L teddler bag

3. Sample injection amount: 200 mL of the composition of the present invention

4. Test instrument: Detector tube gas meter (GV-100S, Gastec, Japan)

5. Initial concentration (unit: ppm)

- Ammonia: 100

- trimethylamine: 30

- hydrogen sulfide: 50

- methyl mercaptan: 4

Test Items unit Sample classification Results ammonia % 30 minutes later 99 ammonia % After 1 hour 99 or more Trimethylamine % 30 minutes later 99 or more Trimethylamine % After 1 hour 99 or more Hydrogen sulfide % 30 minutes later 54 Hydrogen sulfide % After 1 hour 70 Methyl mercaptan % 30 minutes later 26 Methyl mercaptan % After 1 hour 34

That is, after the treatment of the composition, 99% or more of ammonia and trimethylamine were removed, 54% of hydrogen sulfide was removed after 30 minutes, 70% of hydrogen sulfide was removed after 1 hour, 26% of methyl mercaptan was removed after 30 minutes, Afterwards, it was confirmed that more than 34% was removed.

Example  4: Confirmation of cultivation result according to the treatment of compost

The compost produced in Example 3 and the control group containing commercially available microbial agents were used for cultivation in strawberries, drop tomatoes and sweet potatoes, respectively.

As a result, as shown in Figs. 2, 3 and 4, when the compost was applied, it showed a better growth rate.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.

Claims (5)

Comprising a microbial fermentation broth as an active ingredient,
The microbial fermentation broth may contain,
A step (step 1) of making charcoal for 10 to 12 days in an inactivated state of oak, pine, oak, paulownia, and chaff in which the thermophilic microorganism has been inactivated;
The oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal from which the thermophilic microorganism has been inoculated is taken out from the bulgum, For 36-48 hours (step 2);
A step (step 3) of mixing the mud left in the inhaler and inoculated with the thermophilic microorganism on each of the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal;
(Step 4) of covering the first rice straw on the oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal and chaff charcoal mixed with the mud which is inoculated with the thermophilic microorganism and inactivated;
Mixing the fermented oak charcoal, pine charcoal, ash wood charcoal, paulownia charcoal, and chaff charcoal to prepare a fermented product (Step 5);
A step of mixing the loess and the second rice straw and aging the rice while supplying oxygen in the aeration tank (step 6); And
And a step of mixing the fermented product with the fermented product and aging in an aeration tank (step 7).
delete The method according to claim 1,
Wherein the thermophilic microorganism is selected from the group consisting of Lactobacillus acidophilus , Bifidobacterium bifidum , Streptococcus thermophilus and Bacillus firmus A composition for removing odor, characterized by being at least one microorganism.
The method according to claim 1,
The microbial fermentation broth is prepared by mixing and aging a first microorganism preparation prepared by mixing lacquer, green tea leaves, and bamboo stalks, a second microorganism preparation mixed with agar, A composition for removing odor characterized by being able to make and add a third microorganism preparation.
5. The method of claim 4,
1 to 5 parts by weight of a third rice straw is mixed with 100 parts by weight of a fourth microorganism preparation in which the first microorganism preparation, the second microorganism preparation and the third microorganism preparation are mixed at a weight ratio of 1: 1: 1, followed by aging By weight based on the total weight of the composition.

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