CN103783093A - Composition having functions of sterilization and nematode prevention and control - Google Patents
Composition having functions of sterilization and nematode prevention and control Download PDFInfo
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- CN103783093A CN103783093A CN201410090706.1A CN201410090706A CN103783093A CN 103783093 A CN103783093 A CN 103783093A CN 201410090706 A CN201410090706 A CN 201410090706A CN 103783093 A CN103783093 A CN 103783093A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a composition having functions of sterilization and nematode prevention and control. The composition comprises the following raw materials by weight: 1-60% of vegetable oil, 0.1-0.5% of antioxidant, 10-30% of surfactant and the balance of volatile organic compound (VOC) solvent. The composition having functions of sterilization and nematode prevention and control solves the technical problem that the vegetable oil cannot be applied to soil, is free of toxicity and pollution to the environment, and belongs to environment-friendly type preparations. According to the composition, the vegetable oil and other components are mixed to prepare the liquid preparation which can be applied to soil to kill bacteria and nematode conveniently, the antioxidant can protect active ingredients of the composition, the surfactant enables the vegetable oil to disperse in water uniformly to form stable emulsion, and the solvent has a dilution function.
Description
Technical field
The present invention relates to a kind of composition, particularly a kind of composition with sterilization, the effect of control nematode, belongs to agricultural pest prevention and control field.
Background technology
The disease of the five disasters controls that Zhi Bing circle generally believes is soil-borne disease, vascular bundle diseases, virus disease, bacteriosis and nematodiasis, and soil-borne disease extensively, is seriously listed in first of the disease of this five disasters control due to harm.The control of the soil-borne disease very long course of having passed by, from the soil disinfectant of killing livestock property if the application development of chloropicrin, Celfume and various sterilization agent is to biological control, but it is rampant that the propagation of soil-borne disease and harm are still advocated, and this is to be worth us to ponder deeply and the problem of further investigated.
Root and the stem of soil-borne disease main harm crop, once plant growth disease occurs at early stage, seedling root rot or stem rot are rotten, seedling will soon be dead, has a strong impact on plant growth.Plant growth deuterogenesis disease, general time underproduction 20%-30%, serious time underproduction 50%-60%, even total crop failure, after soil-borne disease occurs, more difficult control, germ is ensconced in soil and survives the winter, and is difficult to be killed, continue infringement crop the coming year, so circulation, disease is more and more serious.
Soil nematodes and disease have substantial connection.Soil nematodes can be caused the wound of root system of plant, favourable infection process and disease is increased the weight of, and often nematode and fungal disease occur simultaneously, as inseparable with soil nematodes in cotton wilt, are called the compound disease of withered-nematode in american cotton fusarium wilt.
Nematosis is in the many plants including crops and forestry (pine wood nematode), and the whole world is because of eelworm harm and annual approximately 1,000 hundred million dollars according to estimates of the losses that cause to agricultural production of the compound disease therefore causing.Particularly, in greenhouse and booth, Plant nematode life span is long, and density is large, brings serious harm to horticultural crop.Therefore harm how to control and eliminate Plant nematode has caused widely in worldwide to be paid attention to.At present plant root-knot nematode main prevented and treated method or particularly high-toxic pesticide control of chemical control, and as Aldicarb, Furadan, Celfume, chloropicrin, metham-sodium etc., wherein a lot of provinces are disabled at home for Furadan; Celfume is disabled in developed country in 2005, estimates will to be forbidden in developing country for 2015 comprehensively; Chloropicrin a lot of crops as disabled on Chinese cabbage etc.These high malicious chemical pesticides, directly pass through in fruit or water, atmosphere arrival people, animal body, or finally pass to people, animal by environment, food chain; In addition, because soil pollution has disguise, latency and chronicity, it forms serious harm by vegeto-animal food chain to human health, thereby be difficult for being discovered by people, if the acute toxicity Oral Administration in Rats LD50 of Aldicarb is that 0.65 mg/kg of oral LD50 of mouse is 0.30 mg/kg.Avermectin has good eelworm-killing activity, especially B2a composition, but its formulation products containing dimethylbenzene, DMF and the contour malicious organic solvent of pyrrolidones is applied to agricultural, in control nematode, has caused very large pollution.
Under System of Modern Agriculture, a large amount of uses of hypertoxic chemical pesticide have brought serious problem to agricultural production, food security and environmental protection etc.Therefore, a kind of effective, environmentally friendly, easy-to-use novel formulation of society need, is sustainable agriculture service.
Summary of the invention
Object of the present invention is just for above-mentioned the deficiencies in the prior art, and the sterilization of a kind of safety, environmental protection, the composition of control nematode are provided, and it is not containing toxicity, and environmentally safe, belongs to environmental type preparation.
To achieve these goals, the technical solution used in the present invention is such:
There is a composition for sterilization, the effect of control nematode, comprise the raw material of following percentage by weight: vegetable oil 1-60%, antioxidant 0.1-0.5%, surfactant 10-30%, surplus is low VOC solvent.
Above-mentioned vegetable oil is one or more in cinnamon oil, eucalyptus oil, caryophyllus oil.
Above-mentioned low VOC solvent is one or more in Canola oil, soybean oil methyl esters, turpentine oil, methyl oleate.
Above-mentioned antioxidant is 2,6-toluene di-tert-butyl phenol (BHT), TBHQ (TBHQ), butylated hydroxy anisole (BHA), eugenol, Moschus grass meal, one or more in vanilla phenol.
Above-mentioned surfactant is agriculture breast 500# and Nongru-700 #.
Eucalyptus oil, chief component: 1, 8-Cineole (more than 80%), amphene, phellandrene, terpineol, acetic acid geraniol, isopentyl aldehyde citronellal and piperitone etc. are colourless or micro-yellow liquid.Be peculiar refrigerant folium eucalypti fragrance band camphor smell somewhat, be with a little medicine gas, have peppery mouthful of refrigerant sense, fragrance is strong and lasting.The purposes eucalyptus oil of eucalyptus oil by purposes can medicinal oil, three kinds of oil and iundustrial oils for spices.Be Eucalyptus Globulus oil pharmaceutically applying more, Eucalyptus Globulus oil has the effect of anti-various bacteria, particularly, to the infection of the upper respiratory tract, chronic bronchitis, has phlegm-dispelling functions.By bacteriostatic experiment, find that blue gum leaf oil has bacteriostasis clinically, while splashing into two methods application by suction and tracheae, treatment pulmonary tuberculosis patient is had to gratifying effect.Eliminating common sympton, especially cavity is closed is seemingly above good compared with other antituberculosis drugs thing.Eucalyptus Globulus oil also can be used for some skin disease, and as irrigation, deodorant and the neuropathic antalgesic of wound face, ulcer, fistula.In addition Eucalyptus Globulus oil is also usually used in 10 drops, cooling ointment, essential wind oil, mosquitofugal oil, pectoral etc.Eucalyptus citriodora oil uses in spices and daily chemical products, and eucalyptus citriodora oil contains citronellal, can be used for menthol-dl, and thymol essence also can be isolated rhodinol, lemon alcohol, for preparing perfume, perfumed soap, essential oil, prickly-heat powder etc. from eucalyptus citriodora oil.Industrial eucalyptus oil can be used for flotation ore in sand form, additive in detergents, surfactant and bactericide.Eucalyptus oil is a kind of natural detergent, if the kind fabric of clothes, carpet has chewing gum to be difficult to wash off conventionally, it is found that recently, makes washing agent be very easy to clean this type of dirt mark with eucalyptus oil.Further investigation is discovery again, and eucalyptus oil has higher surface-active and sterilizing ability, and source is abundant, nuisanceless generation, really a kind of very good naturally occurring emulsifying agent and bactericide.
Cinnamon oil is the volatile oil that the dry bark of canella Chinese cassia tree, leaf or branch obtain through extraction, has Xin Lie and warm sweet fragrance, and band is burnt, wooden, cream is fragrant.In cinnamon oil, contain a lot of important functional components, it more than 80% is wherein cinnamic acid, contain in addition tens kinds of above compositions such as cinnamyl alcohol, cinnamic acid, coumarin, acetic acid cassia bark ester, acetophenone, can be used as natural spices, antioxidant, bacteriostatic agent and preservative.Therefore, can be used widely in industries such as food, medicine, chemical industry.
Caryophyllus oil, colourless or pale yellow liquid, has strong cloves fragrance, water insoluble.Be mainly used in antibacterial, hypotensive: also can be used for, in perfume, essence and various cosmetic essence and fragrance for detergents formula, can also being used for the allotment of flavoring essence.
One or more that the vegetable oil using in the present invention is cinnamon oil, eucalyptus oil, caryophyllus oil, there is the effect of sterilization and control nematode, they are used widely in industries such as food, medicine, chemical industry, and its toxicity is low, clearly indicate and avoid registration at U.S. EPA 40 CFR 152:25B.Although being acknowledged as, the vegetable oil that the present invention uses there is sterilization and nematicide activity, but, their high volatile volatile and low-solubility have limited their uses in soil, therefore vegetable oil and other component are mixed and made into liquid preparation by the present invention, is convenient to be applied to soil sterilization and kills nematode.Adding of antioxidant played to the active ingredient of the present composition effect of protecting, and surfactant makes vegetable oil stable emulsion of dispersed formation in water, and solvent plays diluting effect.And the solvent that the present invention uses is for for low VOC solvent, to ecotope without harm.
Therefore, the invention solves vegetable oil and can not be applied to the technical problem of soil, a kind of composition that has sterilization simultaneously and kill nematode double action is provided, it is containing toxicity, and environmentally safe, belongs to environmental type preparation.
Embodiment
In order more clearly to understand object of the present invention, technical scheme and beneficial effect, below the present invention is described further, but protection scope of the present invention is not limited in following examples.
The present invention is a kind of has the composition of sterilization, the effect of control nematode, comprises the raw material of following percentage by weight: vegetable oil 1-60%, and antioxidant 0.1-0.5%, surfactant 10-30%, surplus is low VOC solvent.
Above-mentioned vegetable oil is one or more in cinnamon oil, eucalyptus oil, caryophyllus oil.
Above-mentioned low VOC solvent is one or more in Canola oil, soybean oil methyl esters, turpentine oil, methyl oleate.
Above-mentioned antioxidant is 2,6-toluene di-tert-butyl phenol (BHT), TBHQ (TBHQ), butylated hydroxy anisole (BHA), eugenol, Moschus grass meal, one or more in vanilla phenol.
Above-mentioned surfactant is agriculture breast 500# and Nongru-700 #.
Composition of the present invention, preferential preparation group is as follows: (by weight percentage)
Test with above-mentioned preparation:
One, LC50 and the LC90 of in vitro root-knot nematode J2
1, test objective: measure LC50 and LC90 to root-knot nematode J2.
2, experimental condition
2.1 for trying target: root-knot nematode Meloidogyne incognica
2.2 instrument and equipments: the graceful funnel of shellfish, organize pulper, sieve, counting culture dish, magnetic stirrer, liquid-transfering gun, centrifuge, suction pipe, high-pressure nozzle, anatomical lens, nematode is chosen pin.
3, test method
Get 1 ml root-knot nematode second instar larvae suspension, containing root-knot nematode second instar larvae 30-50 bar, under stereomicroscope, detect the J2 borer population of living, add medicament, while making nematode and medicament be 30min time of contact, observe residue nematode borer population alive.
4, result of the test
Two, the LC50 in vitro pine wood nematode and LC90
1, test objective: measure LC50 and LC90 to pine wood nematode.
2, experimental condition
2.1 for trying target: pine wood nematode Bursaphelenchus xylophilus
2.2 instrument and equipments: number culture dish, magnetic stirrer, liquid-transfering gun, centrifuge, anatomical lens, nematode is chosen pin.
3, test method
Get pine wood nematode nematode suspension, every processing containing nematode 40-60 bar, detects the borer population of living under stereomicroscope, add medicament, observes residue nematode borer population alive while making nematode and medicament be 0.5 h and 1 h time of contact.
4, result of the test
Three, to root-knot nematode egg and the potted plant test of pesticide effectiveness of second instar larvae
1, test objective:
Root-knot nematode egg, Ji Er Age larva preventive effect filters out best medicament and spraying medicine concentration, and is killed line activity with commercially available medicament 1.8% Avermectin (dimethylbenzene) and compared.
2, experimental condition
2.1 for examination target with for studying thing
Tomato seedling (3-4 leaf), Meloidogyne incognita (Meloidogyne incognica), contrast medicament: 1.8% abamectin emulsifiable concentrate
2.2 condition of culture
The soil texture (sand: soil=2:1), greenhouse temperature (15-34 ℃)
3, experimental scheme
(1) dress basin: cut-off footpath is that 30cm plastic flowerpot is filled soil (every basin soil homogeneous band has line eggs and two Age larvas), and injects 400ml water to keep ground moistening;
(2) pretreatment (1 time): before transplanting, by each medicament dilute in proportion, the 1st perfusion, every basin perfusion amount is 200 ml, each processing repeats for 3 times;
(3) transplant: pretreatment, after 10 days, is transplanted tomato seedling (young plant size evenly), and the final singling of watering;
(4) post processing (2 times): transplant after 2 days, the 2nd perfusion (treatment agent is consistent with pretreatment), every basin perfusion amount is 200 ml; After 10 days, carry out the 3rd perfusion (treatment agent is consistent with pretreatment) the 2nd perfusion.
(5) record data: after 2 months, button basin results plant, measures plant height and carry out disease scale and take pictures, and plant overground part is cut gently, records the fresh weight of the each repeatedly top of each processing and root.According to blank incidence, root eelworm harm situation is carried out to classification investigation, adopt following grade scale:
0 grade: without root knot
1 grade: root knot area accounts for below 25% of whole area
2 grades: root knot area accounts for the 26-50% of whole area
3 grades: root knot area accounts for the 51-75% of whole area
4 grades: root knot area accounts for the more than 75% of whole area
4, test data
Four, the potted plant test of pesticide effectiveness to root-knot nematode egg and second instar larvae
1, test objective: measure line eggs Ji Er Age larva preventive effect
2, experimental condition
2.1 for examination target with for studying thing:
Tomato seedling (3-4 leaf), Meloidogyne incognita (Meloidogyne incognica)
2.2 condition of culture
The soil texture (sand: soil=2:1), greenhouse temperature (18-34 ℃)
3, experimental scheme
October 6, cut-off footpath was that 6 cm nutritive cubes fill 150 ml soil, and inoculum concentration is 2000 root-knot nematode eggs and second instar larvae.Carry out following pretreatment: October 7 processed for the first time, October 14 processed for the second time, and every basin formulation rate is 40 ml, transplanted tomato seedling (4-5 leaf) on October 21.Button basin results plant, measured plant height and carried out disease scale November 12.Plant overground part is cut gently, recorded the fresh weight of the each repeatedly top of each processing and root.
4, test data
Five, the indoor biometrics test to Fusarium oxysporum
1, test objective
The indoor bactericidal activity of test to Fusarium oxysporum. Fusarium oxysporum (Fusarium.oxysporum) is a kind of universal soil-borne disease fungal pathogens, host range is extensive, can cause the generation of the 100 various plants fusarium wilts such as melon, Solanaceae, banana, cotton, pulse family and flowers.
2, test method and step: adopt growth hormone rate determination method
Malicious medium (each group all configures 50ml) is with in 2.1 preparations
Will be for reagent liquid according to each for reagent product final concentration, be diluted to a series of concentration with sterile distilled water, the liquid of accurately drawing respectively 0.5ml joins the 50ml medium dissolving.(medium is cooled to 55 ℃ of left and right to add again) mixes, and obtains the band poison medium of ultimate density, pours the culture dish of sterilizing into, and each gradient is established four repetitions, cooling with malicious solid culture base plane.
2.2 inoculation pathogens
Get band bacterium culture medium post with the card punch of 0.5cm, be then put in and be with in malicious medium plane with transfer needle picking bacterium cake.Each culture dish is put a bacterium cake in centre, mycelia faces up.
2.3 cultivate
The medium of having inoculated is placed in to constant incubator and cultivates, 28 ℃ of temperature, but because indoor temperature reduces, actual cultivation temperature is between 26 ~ 27 ℃.
2.4 result inspection and method for expressing thereof
72h after pure culture biscuits involvng inoculation, takes out culture dish and measures colony diameter (representing with mm).2 diameters are surveyed in each bacterium colony right-angled intersection, represent bacterium colony size with its mean.Substitution formula is obtained and is suppressed pathogen growth rate:
Inhibition growth rate=[1-(processes colony diameter-bacterium cake diameter)/(contrast colony diameter-bacterium cake diameter)] ﹡ 100%
3, result of the test
The explanation of above example, the sterilization of the present composition and to kill nematode effect remarkable.
Claims (5)
1. a composition with sterilization, the effect of control nematode, is characterized in that: said composition comprises the raw material of following percentage by weight: vegetable oil 1-60%, and antioxidant 0.1-0.5%, surfactant 10-30%, surplus is low VOC solvent.
2. a kind of composition with sterilization, the effect of control nematode according to claim 1, is characterized in that: described vegetable oil is selected from cinnamon oil, eucalyptus oil and caryophyllus oil.
3. a kind of composition with sterilization, the effect of control nematode according to claim 1, is characterized in that: described low VOC solvent is selected from Canola oil, soybean oil methyl esters, turpentine oil and methyl oleate.
4. a kind of composition with sterilization, the effect of control nematode according to claim 1, it is characterized in that: described antioxidant is selected from 2,6-toluene di-tert-butyl phenol (BHT), TBHQ (TBHQ), butylated hydroxy anisole (BHA), eugenol, Moschus grass meal and vanilla phenol.
5. a kind of composition with sterilization, the effect of control nematode according to claim 1, is characterized in that: described surfactant is selected from agriculture breast 500# and Nongru-700 #.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105325473A (en) * | 2015-11-17 | 2016-02-17 | 安徽云溪农业有限公司 | Plant extract composition with red spider and airborne fungus killing activity |
| CN105360110A (en) * | 2015-11-17 | 2016-03-02 | 安徽云溪农业有限公司 | Plant extract composition having nematode and soil-borne fungi killing activity |
| CN106070359A (en) * | 2016-07-05 | 2016-11-09 | 山东省农作物种质资源中心 | A kind of plant seed germination box sterilization coating agent |
| CN106106571A (en) * | 2016-06-23 | 2016-11-16 | 袁春华 | A kind of method utilizing Gourmet Powder Factory's waste liquid compound botanical oil to prepare disinfectant use in agriculture |
| CN110973171A (en) * | 2019-12-13 | 2020-04-10 | 陕西速源节能科技有限公司 | Environment-friendly pesticide reagent and preparation method thereof |
| CN111150688A (en) * | 2020-01-10 | 2020-05-15 | 广州贝玛生物科技有限公司 | Mosquito-repellent prickly heat-removing spray and preparation method thereof |
| CN113749130A (en) * | 2021-08-03 | 2021-12-07 | 广西化工研究院有限公司 | Sugarcane biological seed stem coating agent and preparation method thereof |
| CN113796394A (en) * | 2021-10-25 | 2021-12-17 | 宜宾樟之美生物科技有限公司 | Mixed use method of biological preparation containing eucalyptus oil and biological insecticide |
| CN115161240A (en) * | 2022-07-25 | 2022-10-11 | 四川龙蟒福生科技有限责任公司 | Bacillus velezensis strain and application thereof |
| CN115677408A (en) * | 2022-11-01 | 2023-02-03 | 西安德农生物科技有限公司 | Traditional Chinese medicine compound fertilizer for promoting crop absorption and preparation method thereof |
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| CN105325473A (en) * | 2015-11-17 | 2016-02-17 | 安徽云溪农业有限公司 | Plant extract composition with red spider and airborne fungus killing activity |
| CN105360110A (en) * | 2015-11-17 | 2016-03-02 | 安徽云溪农业有限公司 | Plant extract composition having nematode and soil-borne fungi killing activity |
| CN106106571A (en) * | 2016-06-23 | 2016-11-16 | 袁春华 | A kind of method utilizing Gourmet Powder Factory's waste liquid compound botanical oil to prepare disinfectant use in agriculture |
| CN106070359A (en) * | 2016-07-05 | 2016-11-09 | 山东省农作物种质资源中心 | A kind of plant seed germination box sterilization coating agent |
| CN106070359B (en) * | 2016-07-05 | 2018-09-14 | 山东省农作物种质资源中心 | A kind of plant seed germination box sterilization coating agent |
| CN110973171A (en) * | 2019-12-13 | 2020-04-10 | 陕西速源节能科技有限公司 | Environment-friendly pesticide reagent and preparation method thereof |
| CN111150688A (en) * | 2020-01-10 | 2020-05-15 | 广州贝玛生物科技有限公司 | Mosquito-repellent prickly heat-removing spray and preparation method thereof |
| CN113749130A (en) * | 2021-08-03 | 2021-12-07 | 广西化工研究院有限公司 | Sugarcane biological seed stem coating agent and preparation method thereof |
| CN113796394A (en) * | 2021-10-25 | 2021-12-17 | 宜宾樟之美生物科技有限公司 | Mixed use method of biological preparation containing eucalyptus oil and biological insecticide |
| CN115161240A (en) * | 2022-07-25 | 2022-10-11 | 四川龙蟒福生科技有限责任公司 | Bacillus velezensis strain and application thereof |
| CN115161240B (en) * | 2022-07-25 | 2023-10-24 | 四川龙蟒福生科技有限责任公司 | Bacillus velezensis strain and application thereof |
| CN115677408A (en) * | 2022-11-01 | 2023-02-03 | 西安德农生物科技有限公司 | Traditional Chinese medicine compound fertilizer for promoting crop absorption and preparation method thereof |
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