KR20110029297A - A natural pesticidal composition and pesticidal method using the same - Google Patents

Abstract

PURPOSE: A natural insecticide is provided to effectively eradiate harmful insects without harm to human body. CONSTITUTION: An insecticide composition contains essential oil extracted from Thuja occidentalis L. The insecticide composition further contains surfactant, nonyl phenol-10 mole-phosphate. A method for preparing the essential oil comprises: a step of collecting and pulverizing leaves of Thuja occidentalis L.; a step of mixing the pulverized leaves with 125g of distilled water and extracting for 3 hours; and a step of cooling for one hour.

Description

Natural Insecticide Composition and Insecticide Method Using the Same {A Natural Pesticidal Composition and Pesticidal Method Using The Same}

The present invention relates to a natural pesticide composition that is both environmentally friendly and harmless to humans while at the same time eradicating pests of crops.

Peach aphid, Myzus persicae , belongs to the Homoptera aphid, with over 66 and 300 host species recorded worldwide (Shim et al., 1977). Plant damage caused by peach aphids absorbs the tissues of young plants, direct damages such as stress on fire, leaf shrinkage, growth rate and slowing down, and yield reduction, as well as the mediation and secondary sooting of over 100 plant virus diseases. Induction of disease, and the like.

Peach aphids in Korea are cabbage and radish. It causes damage to vegetable crops such as cabbage, lettuce, pepper, watermelon and fruit crops such as peach and flower crops such as symbidium and chrysanthemum. In addition to spring-cultivated Chinese cabbage and radish, the peach aphid is increasing year by year in the summer, such as Daegwallyeong, and cabbage and radish.

The recent well-being phenomenon has increased the consumer's interest in the safety of agricultural products and foods, the need to produce safer agricultural products than ever before.

In addition, due to the excessive use of chemical pesticides, environmental problems such as soil disruption, water pollution and pesticide residues of soil and agricultural products have emerged as a big social issue due to excessive use of chemical pesticides. There is an urgent need for low or no toxic, pollution-free natural or biological pesticides.

Chemical pesticides that have been in use for decades have become more resistant to pests and, as time passes, more pesticides or lethal pesticides can be used to control them, making environmental problems worse. In addition, it causes serious social problems, which are fatal hazards to the human body that consumes crops directly. As a result, researches on biopesticides such as natural pesticides and natural microorganisms to replace chemical pesticides are being actively conducted, but most natural pesticides and natural microorganisms have a weak control effect or extremely limited effects, and are expensive. Since it is not effective and economically unfavorable to farmers, it has not succeeded as a substitute for chemical pesticides to realize eco-friendly agriculture.

Western cypress ( Thuja) occidentalis L. ) is an evergreen coniferous tree belonging to the family Cypressaceae, also called the American cypress, native to North America, 20m high, 60cm in diameter, fragrant. The bark is light yellowish brown, thinly peeled, and the branches spread horizontally. The leaves suddenly peak in the shape of eggs. The leaves attached to the stem are 3 mm long and occupy the branch, and the leaves of the branch are not occupied. The surface is light green and the back is yellowish green. It is mainly distributed in the sub-central region of Korea, and its leaves are used for hemostasis, diuresis, and seeds for nourishment and sedatives. The cypress contains volatile essential oils. Terpene compounds found in essential oils can be divided into monoterpenes with a carbon-10 backbone, sesquiterpenes with a carbon-15 backbone, and diterpenes with a carbon-20 backbone. These can be divided into alcohols, aldehydes, ketones, ethers, esters, acids and oxides, depending on their functional groups. Essential oils are known to contain small amounts of monoterpenes. The essential oil components extracted from the cedar have various physiological activities such as lowering blood cholesterol concentration, sedative effects when inhaled essential oils, antibacterial and insecticidal effects, and plant growth regulation.

As mentioned above, although the cypress essential oil has various physiological activities, research and development on the use of biopesticides are insufficient.

According to the present invention, the cypress essential oil has a unique strong insecticidal power and has the potential to be used as an environmentally friendly insecticide.

Therefore, the present invention relates to the development of environmentally friendly biological pesticides that are excellent for the removal of peach aphids without harming the crops.

It is an object of the present invention to effectively provide a pesticide composition that is environmentally friendly and harmless to humans, which can replace chemical pesticides having the aforementioned side effects.

One aspect of the present invention relates to an insecticide composition comprising an extract extracted from a cypress.

The extract is a substance extracted from the cypress, it is not particularly limited as long as it has a pesticidal effect against pests, preferably may be essential oils. The extract may have a content of 1 to 15% (v / v), more preferably 15% (v / v), relative to the total pesticide composition volume.

In addition, the present invention may further include a surfactant in the extract. The surfactant may be used as long as it is a surfactant generally used in pesticide compositions, preferably Tween # 20, dimethyl tetradecylamine oxide, nonyl phenol-10 mole-phosphate (Nonyl phenol) -10 mole-phosphate), sodium lauryl ether sulfate (SLES) and cocoamide alkyl-dimethylamine oxide (Cocoamide alkyl-dimethylamine oxide), and more preferably nonyl phenol-10 mole-phosphate may be nonyl phenol-10 mole-phosphate.

The surfactant may have a content of 6 to 10% (v / v), preferably 8% (v / v), based on the total pesticide composition volume.

Yet another aspect of the present invention relates to an insecticide composition comprising a constituent compound of essential oil extracted from a cedar as an active ingredient.

The constituent compounds are essential oils extracted from the cypress, α-pinene, camphene, β-pinene, myrcene, para-cymene, d-isothujone, α-thujone, camphor, terpin-2-ol, Terpinyl acetate and bonyl acetate, and the like, and preferably terpinyl acetate or bonyl acetate.

The constituent compound may be 1 to 15% (v / v) of the total pesticide composition, preferably terpinyl acetate is 10% (v / v), carbonyl acetate (Bonyl acetate) 15% (v / v).

In addition, the constituent compound may further include a surfactant. The surfactant may be used as long as it is a surfactant generally used in pesticide compositions, preferably Tween # 20, dimethyl tetradecylamine oxide, nonyl phenol-10 mole-phosphate (Nonyl phenol) -10 mole-phosphate), sodium lauryl ether sulfate (SLES) and cocoamide alkyl-dimethylamine oxide (Cocoamide alkyl-dimethylamine oxide) may be any one, more preferably Tween # 20, Any one of nonyl phenol-10 mole-phosphate, sodium lauryl ether sulfate (SLES) or cocoamide alkyl-dimethy lamine oxide Can be.

The surfactant may preferably be 6 to 10% (v / v), more preferably 8% (v / v), based on the total pesticide volume.

In addition, one aspect of the present invention relates to a method for insecticide using an insecticide composition comprising an extract extracted from the Cypress, or a constituent compound of these extracts as an active ingredient.

According to the present invention, by utilizing the excellent insecticidal efficacy of the essential oil or the constituents of the essential oil, which is an extract extracted from the cypress, it effectively eradicates pests such as peach aphids, and is environmentally friendly, unlike conventional chemical pesticides, It is harmless to human body.

Hereinafter, the present invention will be described in detail through examples. The following examples are provided to illustrate the present invention, but the scope of the present invention is not limited thereto.

<Examples>

1. Extraction of essential oil derived from Japanese cypress

First, the leaves of the cypress were collected and pulverized for 1 minute, 125g was put in an extractor containing 1L distilled water, extracted for 3 hours, and the yield was investigated. Essential oil extraction was performed using a heating mantle having a capacity of 2Kw. The amount of the essential oil extracted was measured by a gauge attached to the extraction apparatus after cooling sufficiently for 1 hour after the end of extraction. About 4% of essential oil was obtained through the above method.

2. Insecticidal Effects of Pleurotus Aphids from Western White and 2 Conifer Oils

In order to verify the insecticidal properties of the essential oil, the method of Example 1 was extracted from the nectar, cypressaceae cypress, and baekbaek derived essential oil. In the insecticidal assay through fumigation, wet cotton wool was laid on the bottom in a culture bottle (500 ml air volume), cut kidney beans (2 × 2 cm), placed on cotton wool, and 20 peach aphids were placed thereon. Then, a paper strip (6 × 3 cm) was hung on the culture bottle stopper, and 2 μl essential oil was fumigated on the paper strip to verify insecticidality at 3 hour intervals. Insecticidal test was carried out while maintaining the growth temperature of 20 ℃ peach aphid.

As a result, the nectar essential oil showed excellent insecticidal properties compared to the essential oils of the cypress and the nectar. As shown in Fig. 1, the insecticidal power of the nectar essential oil according to the present invention showed 60% insecticidal when 12 hours fumigation, and 100% insecticidal when 18 hours fumigation. On the other hand, the essential oils of the cypressaceae cypress and white flower showed 50% after 12 hours of fumigation, which was similar to that of the essential oil of the cypress, but it took 45 hours to reach 100% insecticide, It is clear that the prickly essential oil has high insecticidal properties. It shows a very strong insecticide as an essential oil, and has been found to be highly available as a pesticide.

3. Investigation of pesticidal active ingredient in pestle essential oil and test of insecticidal effect

GC-MS analysis was performed to analyze the components of the essential oil of the cypress, containing terpenoids. As a result, 19 kinds of terpenoid-based substances were detected in Western white essential oil, as shown in Table 1 below. Most of them were monoterpine (Monoterpene) component showed a difference in content.

[Table 1] Constituents in the essential oil of the cypress

Number compound yield(%) One α-pinene 0.67 2 camphene 1.20 3 β-pinene 2.57 4 myrcene 0.46 5 para-cymene 0.60 6 limonene 0.59 7 l-fenchone 1.49 8 d-isothujone  2.19 9 α-thujone 6.63 10 camphor 4.55 11 terpin-2-ol 2.65 12 l-α-terpineol 0.49 13 fenchyl acetate 0.50 14 linalyl isovalerate 0.87 15 bonyl acetate 2.56 16 terpinyl acetate 1.74 17 citronellyl isobutyrate 1.00 18 rimuene 1.74 19 carveol 1.46

Monoterpine (Monoterpene) for the seven high yield of the terpenoid-based material of Table 1 with fumigation in the same manner as described above 2. was tested for insecticidal properties against peach aphids.

As a result, as shown in Figure 2, four types of monoterpine, namely (-)-bonyl acetate, (±) -α-Terpinyl acetate, β-Thujone, p-carene showed a high insecticide. They showed a difference in insecticidal properties as a monoterpine component.

4. Insecticidal test according to the concentration of essential oil of cypress

Prior to testing the insecticidal properties of terpinyl acetate and bonyl acetate, we examined the pesticidal properties of P. essential oil. The essential oil was confirmed by the pesticidal effect by preparing a composition of each of the concentrations of the thyme essential oil obtained through the method of Example 1. The compositions were prepared in final 5 ml with 1%, 5%, 10% and 15% (v / v) essential oils and distilled water, respectively. The mortality of the peach aphids was investigated by spraying each of the prepared cypress essential oil compositions at a distance of 50 cm into a 500 ml container containing 30 peach aphids.

As a result, as shown in FIG. 3, the prickly pear essential oil showed a difference in insecticidal properties according to the treatment concentration, and the insecticide power increased as the treatment concentration was increased. 15% (v / v) of the prickly pear essential oil treatment was found to have the highest insecticidal effect by killing 100% of all peach aphids within 30 minutes of treatment time.

5. Insecticidal assay of terpinyl acetate

Of the four types of terpenoid-based materials shown in Figure 2 terpineyl acetate (Terpinyl acetate) was prepared by the composition to confirm the pesticidal effect. The test method was the same as 4. above, and the composition was prepared in 5 ml of terpinyl acetate (Terpinyl acetate) and distilled water at concentrations of 1%, 5%, 10% and 15% (v / v), respectively. Each terpinyl acetate composition prepared above was sprayed at a distance of 50 cm into a 500 ml container containing 30 peach aphids to examine the mortality of peach aphids.

As a result, as shown in Figure 4, Terpinyl acetate showed a difference in insecticidal according to the treatment concentration, the higher the treatment concentration was increased insecticide. In particular, in the 10% (v / v) Terpinyl acetate treatment, all peach aphids were killed 100% in 30 minutes, and the insecticidal effect was the best.

6. Insecticidal test of bonyl acetate

In order to test the insecticidal effect of Bonyl acetate in the four types of terpenoids shown in Figure 2, by treating the concentration of bonyl acetate to see the insecticidal effect. Test method described above 4 . Isobonyl acetate in the concentration of 1%, 5%, 10%, 15% (v / v) and the remainder was adjusted to the final volume of 5 ml with distilled water, respectively, to prepare Isobonyl acetate compositions for each concentration. Each prepared Isobonyl acetate composition was sprayed at a distance of 50 cm in a 500 ml container containing 30 peach aphids to investigate the mortality of peach aphids.

As a result, as shown in Figure 5, Isobonyl acetate showed a difference in insecticidal according to the treatment concentration, the higher the treatment concentration was increased insecticide. In particular, 82% peach aphids were killed in 30 minutes after spraying 15% Isobonyl Acetate. However, considering the concentration of terpinyl acetate and the treatment time, the insecticidal effect of isobonyl acetate was slightly lower than that of terpinyl acetate.

7. Searching for Surfactants Suitable for Insecticidal Compositions

To explore surfactants that have a pesticidal effect against peach aphids, T. # 20, dimethyl tetradecylamine oxide, nonyl phenol-10 mole-phosphate, Five surfactants, such as sodium lauryl ether sulfate and cocoamide alkyl-dimethyl amine oxide, were used. The essential oil mixture according to the type of surfactant was prepared by adjusting the final concentration of 1.5 ml with the concentration of 10% of the citrus essential oil, 8% of each of the surfactants, and the rest with distilled water. Experimental method was sprayed at a distance of 50cm to the essential oil mixture in a 500 ml container containing 30 peach aphids to examine the mortality of peach aphids.

As a result, as shown in Figure 6, in the treatment sprayed the essential oil mixture of non-phenol phenol-10 mole-phosphate in the cypress essential oil and the surfactant, 100 in 30 minutes % Peach aphids were deadly and had the best insecticidal effect.

8. Insecticidal assay for peach aphids of optimal composition

The salchungryeok this was used for the 5 kinds of the surface active agent with high 6. Terpinyl acetate 10%. Each Terpinyl acetate composition was prepared by adjusting the final 5 ml volume of terpinyl acetate at 10% (v / v) concentration and 8% of each surfactant with distilled water. Each terpinyl acetate composition prepared above was sprayed at a distance of 50 cm into a 500 ml container containing 30 peach aphids to examine the mortality of peach aphids.

As a result, as shown in Figure 7, Terpinyl acetate 10% and four surfactants nonyl phenol-10 mole-phosphate, sodium lauryl ether sulfate (SLES), cocoa High insecticidal properties were shown in compositions containing 8% (v / v) of midalkyl-dimethylamine oxide (Cocoamide alkyl-dimethylamine oxide). According to Figure 6, 30 minutes in the treatment sprayed a composition containing a nonyl phenol-10 mole-phosphate (Nonyl phenol-10 mole-phosphate), Cocoamide alkyl-dimethylamine oxide as a surfactant In the bay, 95% peach aphids were killed, and in the treatment sprayed with a composition containing Tween # 20 and sodium lauryl ether sulfate (SLES) as a surfactant, 100% peach aphids appeared in 30 minutes. It was lethal.

As described above, it was confirmed that it has a strong insecticide against peach or aphids when including not only the cypress essential oil but also a single component Terpinyl acetate, Bonyl acetate as an active ingredient, confirming the availability as an eco-friendly bio pesticide.

As described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. The scope of the present invention is shown by the claims to be described later rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. Should be.

Figure 1 shows the insecticidal activity against peach aphids during fumigation treatment of three essential oils, such as nectar, cypress, cypress, and cypress, according to the present invention. The strong insecticidal properties of the cypress have been demonstrated.

Figure 2 illustrates the insecticidal properties of four types of monoterpene excellent in insecticidal properties against peach aphids among the terpenoid-based materials of the nectar essential oil according to the present invention.

Figure 3 shows the insecticidal activity against peach aphids according to the concentration of essential oil, such as 1%, 5%, 10%, and 15% (v / v) according to the present invention.

Figure 4 shows the insecticidal properties of terpinyl acetate (Perpinyl acetate) of the terpenoid-based terpenoids of the essential oil of the according to the present invention Peach aphids, such as 1%, 5%, 10%, and 15% (v / v) will be.

Figure 5 shows the insecticidal properties of the peach aphids of bonyl acetate (Bonyl acetate), such as 1%, 5%, 10%, and 15% (v / v) of the terpenoid-based substance of P. essential oil according to the present invention .

FIG. 6 illustrates insecticidal activity against peach aphids according to the types of five surfactants including western side white essential oil to select a surfactant suitable for an insecticide composition.

Figure 7 shows the effect of the optimal pesticide composition containing terpinyl acetate (Terpinyl acetate).

Claims (13)

Insecticide composition comprising an extract extracted from the tree of the tree. The method of claim 1, The extract is a pesticide composition, characterized in that the essential oil. 3. The method of claim 2, The extract is a pesticide composition, characterized in that the content of 1 to 15% (v / v). The method of claim 1, Insecticide composition, characterized in that it further comprises a surfactant in the extract. The method of claim 4, wherein The surfactant is a pesticide composition, characterized in that nonyl phenol-10 mole-phosphate (Nonyl phenol-10 mole-phosphate). The method of claim 4, wherein The surfactant is a pesticide composition, characterized in that the content of 6 to 10% (v / v). Insecticide composition containing the constituent compound of essential oil extracted from a Japanese cedar as an active ingredient. The method of claim 7, wherein The constituent compound is any one selected from the group consisting of terpinyl acetate (Terpinyl acetate) and carbonyl acetate (Bonyl acetate). The method of claim 7, wherein The constituent compound is a pesticide composition, characterized in that the content of 1 to 15% (v / v). The method of claim 7, wherein Insecticide composition, characterized in that it further comprises a surfactant in the constituent compounds. The method of claim 10, The surfactant is Tween # 20, nonyl phenol-10 mole-phosphate, sodium lauryl ether sulfate (SLES) and cocoamide alkyl-dimethylamine oxide (Cocoamide alkyl- dimethylamine oxide) is an insecticide composition, characterized in that any one selected from the group consisting of. The method of claim 10, The surfactant is a pesticide composition, characterized in that the content of 6 to 10% (v / v). A pesticide method comprising using the pesticide composition according to any one of claims 1 to 12.

Images