KR20110122502A - Pesticidal composition comprising plant extract - Google Patents

Abstract

PURPOSE: A composition containing plant extract for repelling mushroom fly is provided to ensure eco-friendly property and to replace agricultural chemical. CONSTITUTION: A composition for repelling mushroom fly contains plant extract. The plant extract is caraway seed extract and cinnamon bark or leaf extract, clove bud or leaf extract, or plant essential oil. The mushroom fly is Camptomyia spp., or Lycoriella ingenue. The composition is used in the form of a cleaning agent, medicine, food additive, and perfume containing 0.01-50 wt% of the composition.

Description

Mushroom fly control composition containing plant extract {Pesticidal composition comprising plant extract}

The present invention relates to a composition for controlling mushroom flies containing plant extracts, and more particularly, to a composition for controlling mushrooms that can be usefully used to control mushroom flies which are representative pests of mushrooms using plant extracts.

Representative pests of mushrooms include mushroom flies, mites and nematodes, and mushroom flies in particular have great damage to mycelia, fruit tissues, and the medium in which mushrooms grow.

Specifically, mushroom flies usually feed on leafy soils, organic grasses, compost heaps, and lumbers in forests, and when cultivation of mushrooms begins, adult insects are attracted to the peculiar smell of hyphae and invade into the cultivation fungus. Spawn on. The hatched larvae grow by ingesting mushroom mycelium and fruiting bodies, multiplying through pupa and adult, and multiplying and damaging the mushrooms.

Mushroom flies occur mainly in the late spring cultivation period, and there was no significant damage during the early stages of cultivation, but nowadays, the growth of mushroom flies has transcended regional and temporal aspects as the area of cultivation increases and the grouping of cultivators. Therefore, it occurs a lot in the whole country.

Among the damages to mushrooms caused by larvae, feeding and cutting the hyphae leads to a decrease in yield, puncturing and cooling the fruiting bodies, and contaminating them, thereby lowering the mushroom's value. In addition, adult insects do not directly infest the mushrooms, but indirect damage occurs by propagating through the pathogens, nematodes, mites, etc. that damage the mushrooms.

In general, the control method of mushroom flies has a method of suppressing adult invasion in the cultivator by installing a 1mm grid screen screen in the entrance and ventilation windows to prevent the invasion of the adult. In addition, when seedlings are planted, 17 g of dust-loaded material (2%) is mixed and treated to effectively prevent the adult invading into the fungus at an early stage. However, Dusban has a fumigation effect, and the invading adult insects have a high control effect, but when an excessive amount of the drug is used, if the condition is good, no harm is caused by the drug. There is a problem that the growth of mycelia is suppressed by the gas generated from the generated gas and rice straw, the yield is reduced.

In addition, by spraying 1,000 times the DDVP emulsion, a strong phosphate-based control agent in the cultivator at weekly intervals during the mycelial growth can control the adult invade in the cultivator, but there is a problem that may be harmful to the human body.

Accordingly, the present inventors completed the present invention by confirming that mushroom flies can be effectively controlled while using a specific plant extract while researching about an eco-friendly composition for controlling mushroom flies while not being harmful to the human body. .

Therefore, the present invention is Nioauli, Lemongrass, Rosemary, Garlic, Marjoram, Cedarwood, Sage, Star anise , Spearmint, Cinnamon, Armoise, Origanum, Wintergreen, Eucalyptus, Cassia, Caraway, Chamomile roman , Thyme white, Thyme, Pennyroyal, Peppermint, Lisea cubeba, Myrtle, Melissa, Savory, Mugwort Wormwood, Yarrow, Bay, Cedarleaf, Coranda, Clove, Pimento berry, Grapefruit, Juniperberry, Tea Tea tree, Fennel, Citrus sp., Cacalia roborowskii, Foeniculum vulgare, Equisetum hyemale, Sessin sarum sieboldii, pine pollen (Pinus densiflora), Ogalpi (Canthopanax sessiliflorus), Schizandra chinensis, clove (Eugenia carrophillata), lemon eucalyptus and lemon ginseng (Codonopsis lanceolata) An object of the present invention is to provide a composition for controlling mushroom flies containing the extracted plant extract.

It is also an object of the present invention to provide a method for controlling mushroom flies, comprising using the composition for controlling mushroom flies.

In order to solve the above problems, the present invention Nioauli, Lemongrass (Lemongrass), Rosemary (Rosemary), Garlic (Garlic), Marjoram (Marjoram), Cedarwood, Sage (Sage) Star anise, spearmint, cinnamon, armoise, origanum, wintergreen, eucalyptus, cassia, caraway ), Chamomile roman, thyme white, thyme, pennyroyal, peppermint, litsea cubeba, myrtle, melissa , Savory, Wormwood, Yarrow, Bay, Cedarleaf, Coriander, Clove, Pimento berry, Grapefruit , Juniperberry, Tea tree, Fennel, Citrus sp., Cacalia roborowskii, Foeniculum vulgare ), Equisetum hyemale, Sesame (Asiasarum sieboldii), Calendula (Pinus densiflora), Ogalpi (Canthopanax sessiliflorus), Schizandra chinensis, Cloves (Eugenia carrophillata), Lemon eucalyptus (Lemon eucalypola donus) It provides a mushroom fly control composition containing a plant extract extracted from one or more plants selected from the group consisting of.

The present invention also provides a method for controlling mushroom flies, comprising using the composition for controlling mushroom flies.

Hereinafter, the present invention will be described in more detail.

In the preparation of the plant extract in the present invention, the whole plant may be targeted, but is not limited thereto. Preferably, plant root, stem, leaf, pulp, flower, fruit, seed, bark and shoot (bud) can be targeted to one or more selected from the group consisting of.

The plant extract is not limited thereto, but may preferably be a vegetable essential oil, and may be a manufactured product or a commercially available product.

In the case of directly preparing the plant extract, any extraction method for extracting the active ingredient from plants known in the art may be used, but is not limited thereto. Preferably, steam distillation using water and / or an organic solvent, cold dipping, At least one method selected from the group consisting of a warming method, a distillation method, a hot water extraction method, a leaching method, a compression method, and a supercritical gas extraction method can be used.

The organic solvent may be at least one selected from the group consisting of hexane, chloroform, acetone, ethyl acetate, and alcohol, but is not limited thereto. The alcohol is preferably a linear or branched alcohol having 1 to 5 carbon atoms, and examples thereof include, but are not limited to, ethanol, methanol, propanol and butanol.

In particular, the Cinnamon bleached oil can be obtained by distilling the refined oil obtained by steam distillation to remove the pigment (carotenoid or flavonoid, etc.) contained in the oil at a temperature of about 110 ℃. In addition, Cassia especial may be an oil produced by adding a process of synthesizing or adding these main ingredients in order to match the content of the main components to the manufacturer's standards, Cassia pure is an oil obtained only by steam distillation, Cassia redistilled is produced by re-distillation of cassia oil, and cassia terpenes may be oil prepared by adding terpins to cassia oil.

In one embodiment of the present invention, plant extracts were prepared by steam distillation using water, or commercial vegetable essential oils were purchased and used. (See <Reference Example 1>.)

In the present invention, the mushroom fly includes both adult and larva, preferably the sid of the larva is characterized by having a black cuticle (Searid), the head is not black cuticle, the body is yellowish white wrinkles Characterized by Porid, cecid, which is characterized by small spots on the body compared to other mushroom flies, and mycetophil, which is similar to mosquitoes, is more preferred. Preferably, those belonging to Blackwing Mushroom Fly (Sciaridae), Cecidomyiidae, Fhoridae, etc. may be representative, but even more preferably mushroom Hogwarts Fly (see FIG. 1) or Long Beard Fly, most preferably. Preferably it may be a larva of the mushroom or flock of mushrooms.

Mushroom fly control composition of the present invention Nioauli (Niaouli), lemongrass (Lemongrass), rosemary (Rosemary), garlic (Garlic), marjoram (Marjoram), Cedarwood, Sage (Sage), star Star anise, spearmint, cinnamon, armoise, origanum, wintergreen, eucalyptus, casaia, caraway, chamomile Chamomile roman, thyme white, thyme, pennyroyal, peppermint, litsea cubeba, myrtle, melissa, melissa Savory, Wormwood, Yarrow, Bay, Bay, Cedarleaf, Coriander, Clove, Pimento berry, Grapefruit, Juniper berry (Juniperberry), Tea tree, Fennel, Citrus sp., Octagon (Cacalia roborowskii), Foeniculum vulgare, Equis etum hyemale, Sessin (Asiasarum sieboldii), Pine pollen (Pinus densiflora), Ogalpi (Canthopanax sessiliflorus), Schizandra chinensis, Cloves (Eugenia carrophillata), Lemon eucalyptus and Codonopsis lance It is characterized by containing a plant extract extracted from at least one selected plant.

Nioauli, Lemongrass, Rosemary, Garlic, Cedarwood, Sage, Star anise, Spearmint, Cinnamon, Armoise, Origanum, Wintergreen, Eucalyptus, Cassia, Caraway, Chamomile Roman, Thyme white ), Extracts from plants selected from the group consisting of thyme, pennyroyal, and peppermint were treated to mushroom flies, preferably mushroom hoax larvae, when treated at 4 ul / cm ² , respectively. It has a 100% control force against (see <Example 1>).

In addition, the plant extracts Licea cubeba (Litsea cubeba), marjoram (Marjoram), Myrtle (Myrtle), Melissa (Melissa), Savory, Wormwood, Yarrow, Bay, Extracts obtained from plants selected from the group consisting of Cedarleaf, Coriander, Clove and Pimento berry are mushroom flies, preferably treated with 4 ul / cm ² , respectively. Crab has more than 90% control against the mushroom larvae larvae (see <Example 1>)

In addition, the extract obtained from the plant selected from the group consisting of grapefruit (Grapefruit), juniperberry, tea tree and fennel among the plant extracts, respectively, treated with 4 ul / cm ² , mushrooms Flies, preferably mushroom control flies, have at least 70% control against larvae (see <Example 1>).

In particular, extracts obtained from the plants of Lemongrass, Thyme, Origanum, Thyme white, Cassia, Clove, Savory and Cinnamon At the level of 0.2 ul / cm ^{2, it} has a strong control of more than 70%, and the extracts obtained from Origanum, Cassia, Clove, Savory and Cinnamon are 0.2 ul. Retains control at concentrations lower than / cm ² .

The caraway plant extract is not limited thereto, but may be preferably extracted from caraway seeds.

In addition, the cinnamon (Cinnamon) plant extract is not limited to this, but preferably may be extracted from cinnamon bark (bark) or leaves, the cinnamon (Cinnamon) plant extract is not limited to this but preferably bleached cinnamon extract ( Cinnamon bleached), the bleached cinnamon extract is a pigment (carotenoid) contained in the oil by heating (for example heated to 100 ℃ to 120 ℃, preferably 110 ℃) cinnamon oil prepared by steam distillation Or flavonoids) may be purified essential oils after removal.

In addition, the Clove plant extract is not limited thereto, but may be preferably extracted from a clove bud or leaf.

In addition, the Cassia plant extract may be, but is not limited to, Cassia especial, Cassia pure, or Cassia redistilled.

The cassia especial is not limited thereto, but preferably, the main components of the cassia oil synthesized or naturally obtained are additionally added to the cassia oil according to a conventional method. In addition, the Cassia pure refers to an essential oil obtained by steam distillation of Cassia according to a conventional method, the redistilled Cassia oil (Cassia redistilled) refers to an oil prepared by re-distillation of the Cassia pure according to a conventional method. .

On the other hand, the Hallabong (Citrus sp.), Octagonal (Cacalia roborowskii), convention (Foeniculum vulgare), Equisetum hyemale, Sessin (Asiasarum sieboldii), pine pollen (Pinus densiflora), Ogalpi (Canthopanax sessiliflorus), Omiza chiza ), Extracts from Eugenia carrophillata and codonopsis lanceolata herbal plants, respectively, have a strong control against mushroom flies, preferably long-tailed mushroom flies larvae (see <Example 1>).

In particular, extracts obtained from octagonal scent, convention scent, sessin, pine pollen, ogalpi, schisandra chinensis, clove and four ginseng herbal plants showed complete control (100%) at 2.0 ul / cm ² treatment, respectively. It showed 100% activity even when treated with / cm ² , and Hallabong and Estuary also had a strong control of 90% or more. (See <Example 1>)

The control composition of the present invention may be used alone for each of the above-described plant extracts, but preferably, when the plant extracts are mixed and used, the control ability is superior. (See <Example 2>)

The composition for controlling the present invention may include the above plant extracts alone, and may further include other pharmacologically acceptable carriers. In this case, the content of the plant extract may be 0.001 to 100% by weight, preferably 0.1 to 50% by weight.

However, the content of the plant extract is not limited to the above values, it is more preferably adjusted according to the method of use and formulation. The usable carrier may be materials included in conventional control agents, such as excipients or diluents, for example, but not limited to saline, buffered saline, dextrose, isoparaffin, water, glycerol and ethanol.

Formulation of the composition for the control of the present invention can be appropriately adjusted according to the method of use and use, for example, liquids, suspensions, granules, warnings, fragrances, powders, syrups, aerosols, extracts, ointments, fluid extracts, Emulsions, premises, acupuncture, eye drops, tablets, injections, spirits, capsules, creams or pills.

The control composition of the present invention is used for controlling mushroom flies, and can be used as a detergent, a medicament, a food additive, and a fragrance. At this time, the composition for the control may be included in 0.01 to 50% by weight.

On the other hand, the control method of the mushroom fly of the present invention includes using the mushroom fly control composition for crops.

The crop includes all the plants that can grow mushroom flies, and preferably may be mushrooms.

The mushroom fly can be controlled by treating the crop composition with the control of the present invention, and a preferred method is to apply the control composition so that mushroom flies or larvae can be in direct contact. Method can be used. The spraying amount or coating amount can be easily determined by those skilled in the art according to the number of mushroom flies or larvae, but is not limited thereto. Preferably, the spraying amount is 0.05 ul / cm ³ based on a unit space. The coating amount is 0.04 ul / cm ² based on the unit area. Above, preferably 0.064 ul / cm ² Above, more preferably 0.096 ul / cm ² More preferably 0.2 ul / cm ² It may be abnormal.

Specifically, the method of spraying the composition for the control of the present invention directly, a method of applying by mixing with the medium, a method of mixing and processing with the fungal inoculum, the site of inoculating the bacteria (usually a hole in the oak and inoculated mushrooms by inoculation As time goes on, mushrooms grow, and this is where the appropriate formulations (granules, tablets, etc.) can be inserted, mushroom growers can be mixed or irrigated into soil, moisture absorbed or sprayed onto appropriate carriers such as nonwoven fabric / paper Mushroom flies can be controlled by placing them around doorways or vents.

The composition for controlling mushroom flies of the present invention is environmentally friendly by containing an extract derived from a specific plant as an active ingredient, and can effectively control mushroom flies, and can be usefully used to replace a pesticide containing the compound as an active ingredient.

Figure 1 is a photograph showing the larvae (left), adult males (middle), adult females (right) of the mushroom hog fly.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

< Reference Example  1>

Preparation of Experimental Materials

<1-1> Preparation of Vegetable Essential Oil

Plant essential oil of Table 1 used in the present invention was used was supplied from the US Burje (Berjё, Bloomfield, NJ).

Most plant essential oils can be obtained through steam distillation methods known in the art. However, Cinnamon bleached oil may be obtained by distilling the essential oil obtained by steam distillation to purify by removing the pigment (carotenoid or flavonoid, etc.) contained in the oil at a temperature of about 110 ℃. In addition, Cassia especial is an oil produced by adding a process of synthesizing or synthesizing these main components from natural to add the content of the main components to Cassia oil. Cassia pure is obtained only by steam distillation and cassia redistilled. The oil obtained was produced by re-distillation. Cassia terpenes were prepared by adding terpins to the obtained Cassia oil.

In addition, the plants of Table 2 used in the present invention were purchased from the herbal medicine in Cheongnyangni Gyeongdong market. The plant was dried and finely ground to make a powder, and then, 600 g of each powder sample was taken into a 1000 ml Erlenmeyer flask, and distilled water was poured into 600 ml to sufficiently immerse the sample. Steam distillation at 100 ° C. for 2 hours and cooling with a cooler yielded each vegetable essential oil. Anhydrous sulfate was used to remove the water in the vegetable essential oil and stored at 4 ° C. for use in this experiment.

<1-2> Preparation of Mushroom Flies

Mushroom homp flies visited three shii farms in Cheonan-si, selected contaminated shiitake trees, transferred them to the laboratory, and then removed them by visual observation of the larvae inhabiting the wood. It was used after.

In addition, long bearded mushroom flies were collected from cultivated oyster mushroom farms in Gapyeong, Gyeonggi-do and induced spawning in PDA (Potato Dextrose Agar) medium. Only the egg yolk mushroom eggs scattered in the medium were selected and inoculated again with Pleurotus eryngii spawn (Spring 2) and transferred to the culture medium at 22 ° C. and 60% or more for 3 to 5 days. The established indoor populations were selected and used for the experiment while breeding under 16h: 8h (L: D) light conditions.

< Example  1>

Control Effect of Vegetable Essential Oil on Mushroom Fly

In order to confirm the control effect of the vegetable essential oil prepared in the <Reference Example 1> against the mushroom fly, the vegetable essential oil prepared in the <Reference Example 1> was quantified in a pipette without diluting in a solvent, 0.8 ul / cm ² or Treated with vegetable oils by treating 0.4 ul / cm ² with 8 mm diameter paper discs and adding 15 ul of water (to prevent the larvae from being affected by rapid drying). After attaching to a 5 ml Bayer lid, the control ability was examined after 24 hours.

When the vegetable essential oil is treated at a concentration of 0.4 ul / cm ² or less, the vegetable essential oil is diluted in 20 ul of ethanol, treated in a horizontal * vertical (3.4 * 0.8 cm) space, and then volatized for 3 minutes, and then 15 ul of water. Was added to investigate the control power in the same way as the high concentration.

The control force was calculated through Equation 1 below.

The control was investigated 24 hours after the treatment and all experiments were carried out in 4 iterations. The larvae were transferred to fresh water for investigation of the control ability, and the subjects without any movement under the microscope (40 times) were regarded as worms, and the results were calculated. The results are shown in Table 1 below.

Control Effect of Vegetable Essential Oil against Mushroom Lump Larva (Unit:%) Vegetable essential oil CAS No. Conc., Ul / cm ² 4 2 0.2 0.096 0.064 0.04 Cedarwood oil 8000-27-9 100 ± 0.0 Sage oil dalmation 8016-64-6 100 ± 0.0 Star anise oil 68952-43-2 100 ± 0.0 57 ± 3.3 Armoise oil 68991-20-8 100 ± 0.0 75 ± 0.0 Peppermint oil 8006-90-4 100 ± 0.0 77 ± 3.3 Chamomile roman oil 8015-92-7 100 ± 0.0 78 ± 1.7 Rosemary oil 84604-14-8 100 ± 0.0 83 ± 3.3 Spearmint oil 8008-79-5 100 ± 0.0 92 ± 1.7 50 ± 0.0 Niaoli oil 8014-68-4 100 ± 0.0 98 ± 1.7 Cinnamon bleached oil 8007-80-5 100 ± 0.0 100 ± 0.0 100 ± 0.0 Eucalyptus oil 8000-48-4 100 ± 0.0 100 ± 0.0 Garlic oil 8000-78-0 100 ± 0.0 100 ± 0.0 Sage oil 8016-65-7 100 ± 0.0 100 ± 0.0 Origanum 8007-11-2 100 ± 0.0 100 ± 0.0 100 ± 0.0 78 ± 1.7 Cassia especial oil 8007-80-5 100 ± 0.0 100 ± 0.0 100 ± 0.0 80 ± 0.0 57 ± 1.7 60 ± 0.0 Cassia redistilled oil 2009-11-23 100 ± 0.0 100 ± 0.0 100 ± 0.0 90 ± 0.0 54 ± 1.7 Cinnamon bark oil 8015-91-6 100 ± 0.0 100 ± 0.0 100 ± 0.0 100 ± 0.0 Pennyroyal 8007-44-1 100 ± 0.0 100 ± 0.0 Caraway seed 8000-42-8 100 ± 0.0 100 ± 0.0 Thyme 8007-46-3 100 ± 0.0 100 ± 0.0 73 ± 3.3 Lemongrass 8007-02-1 100 ± 0.0 100 ± 0.0 87 ± 3.3 Thyme white 8007-46-3 100 ± 0.0 100 ± 0.0 93 ± 1.7 Cassia pure 487-26-3 100 ± 0.0 100 ± 0.0 97 ± 1.7 93 ± 1.3 Wintergreen oil 68917-75-9 100 ± 0.0 100 ± 0.0 Savory oil 8016-68-0 98 ± 1.7 97 ± 1.7 93 ± 1.7 Marjoram oil 8015-01-8 97 ± 3.3 97 ± 3.3 Melissa oil 8014-71-9 97 ± 1.7 80 ± 0.0 58 ± 1.7 Cedarleaf oil 8007-20-3 95 ± 0.0 Coriander oil 8008-52-4 95 ± 0.0 93 ± 1.7 Wormwood 8008-93-3 95 ± 0.0 93 ± 1.7 50 ± 0.0 Yarrow oil 8022-07-9 93 ± 1.7 90 ± 0.0 Lisea cubeba oil 68855-99-2 93 ± 1.7 93 ± 1.7 Clove bud 8015-98-2 90 ± 0.0 90 ± 0.0 83 ± 3.3 Pimento berry 8006-77-7 90 ± 0.0 93 ± 1.7 57 ± 1.7 Myrtle oil 8008-46-6 90 ± 0.0 93 ± 1.7 Bay 8006-78-8 90 ± 0.0 98 ± 1.7 57 ± 1.7 Lemon eucalyptus oil 8000-48-4 90 ± 0.0 98 ± 1.7 Grapefruit oil 8016-20-4 87 ± 3.3 Fennel oil 8016-20-4 87 ± 3.3 77 ± 3.3 Clove leaf oil 8000-34-8 87 ± 3.3 80 ± 0.0 Juniperberry oil 8012-91-7 83 ± 3.3 70 ± 0.0 Tea tree oil 68647-73-4 77 ± 3.3 Cinnamon leaf oil terpenes 8015-91-6 75 ± 0.0 Control 0 ± 0.0 0 ± 0.0 0 ± 0.0 0 ± 0.0 0 ± 0.0 0 ± 0.0

As shown in Table 1, plant essential oils that showed 100% complete control in 4 ul / cm ² and 2 ul / cm ² treated plants were lemongrass, eucalyptus, and thyme. , Origanum, Pennyroyal, Thyme white, Cassia especial, Cassia redistilled, Cinnamon bleached, Cassia pure, Caraway seed Garlic, Cinnamon bark, Sage, Wintergreen essential oils.

As also shown in Table 1 above. Essential oils with more than 90% high control at 4 ul / cm ² include Bay, Wormwood, Clove bud, Savory, Cedarleaf, Coriander and Lee. Sea cubeba (Litsea cubeba), Myrtle (Myrtle), Melissa (Missa), Yarrow (Yarrow) and the like, and also shown in Table 1, essential oils exhibited more than 70% toxicity at 4 ul / cm ² is juniper berry ( Juniperberry, Tea tree, Fennel, Grapefruit, Clove leaf, Cinnamon leaf essential oil terpine.

The plant essential oils tended to decrease rapidly at lower concentrations, but lemongrass, thyme, origanum, thyme white, cassia pure, and clove Clove bud, Savory, and Cinnamon bark essential oils maintain strong control at 70% even at 0.2 ul / cm ² . In addition, Origanum, Cassia especial, Cassia redistilled, Cassia pure, Clove bud, Savory, Cinnamon bark refinery are 0.2 ul / cm Than ² It can be seen that the control power is maintained even at low concentrations.

On the other hand, the control activity results for the bulrush mushroom larvae according to the concentration of the vegetable essential oil extracted from the herbal plants are shown in Table 2 below.

Control Effect of Vegetable Essential Oil Derived from Herbal Plants against Larvae Larvae (Unit:%) Kinds Conc., Ul / cm ² 2 0.2 Hallabong ( Citrus) sp.) 93 ± 2.5 88 ± 2.5 Octagonal Cacalia roborowskii ) 100 ± 0.0 98 ± 2.5 Foeniculum vulgare ) 100 ± 0.0 100 ± 0.0 Equisetum hyemale ) 96 ± 1.7 93 ± 2.5 Sessin ( Asiasarum sieboldii ) 100 ± 0.0 100 ± 0.0 Pinus densiflora ) 100 ± 0.0 93 ± 2.5 Canthopanax sessiliflorus ) 100 ± 0.0 83 ± 2.5 Schizandra chinensis ) 100 ± 0.0 95 ± 2.9 Cloves ( Eugenia) carrophillata ) 100 ± 0.0 100 ± 0.0 Codonopsis lanceolata ) 100 ± 0.0 88 ± 2.5

Vegetable essential oils extracted from herbal plants as shown in Table 2 shows a strong control activity against larvae larvae.

Particularly, as shown in Table 2, the vegetable essential oils exhibiting complete control when treated with 2.0 ul / cm ² are essential oils extracted from octagonal scent, gynecological scent, sessin, pine pollen, ogalpi, schisandra, cloves and ginseng.

In addition, the essential oils that showed 100% activity at 0.2 ul / cm ² diluted 10-fold were synergistic, sessin and cloves, and the other vegetable essential oils, Hallabong and genus, maintained a strong control of more than 90%.

< Example  2>

Control Effect of Mushroom Fly on Mixture of Vegetable Essential Oils

As in <Example 1>, it was confirmed whether the plant essential oils exhibiting a strong control activity can be maximized more than the case of treatment alone as well as the treatment.

Specifically, clove bud (Clove bud) and Cassia (Cassia) especial mixed (1: 1, v / v) and the case of not mixed with the results are shown in Table 3 below.

Control Effect of Mushroom Fly on Mixture of Vegetable Essential Oils Kinds Conc., Ul / cm ² 4.0 ^* (2.0) 2.0 (0.1) 0.2 (0.01) 0.096 (0.048) Mix of clove bud and cassia especial 100 ± 0.0 100 ± 0.0 97 ± 1.7 63 ± 1.7 Clove bud 93 ± 1.7 76 ± 2.4 15 ± 4.4 0 ± 0.0 Cassia especial 100 ± 0.0 100 ± 0.0 63 ± 1.9 34 ± 5.8

^{* The} plant essential oil mixture is the concentration of each essential oil in a 1: 1 ratio, and the parenthesis is the concentration of essential oil alone.

As shown in Table 3, the vegetable essential oil was found to exhibit a higher control ability even if treated in the same concentration compared to the single treatment. In particular, when the treatment at a low concentration it can be seen that the synergistic effect is better when mixed. It can be seen that by mixing the essential oils, the control substances contained in each of the essential oils affect mushroom flies by exerting mutual synergy.

Claims (10)

Niaouli, Lemongrass, Rosemary, Garlic, Marjoram, Cedarwood, Sage, Star anise, Spearmint ), Cinnamon, Armoise, Origanum, Wintergreen, Eucalyptus, Cassia, Caraway, Chamomile roman, Time White ( Thyme white, thyme, pennyroyal, peppermint, litsea cubeba, myrtle, melissa, savory, wormwood, yarrow (Yarrow), Bay, Cedarleaf, Coriander, Clove, Pimento berry, Grapefruit, Juniperberry, Tea tree Fennel, Citrus sp. Plant extracts extracted from one or more plants selected from the group consisting of powder (Pinus densiflora), canthopanax sessiliflorus, Schizandra chinensis, clove (Eugenia carrophillata), lemon eucalyptus and lemon ginseng (Codonopsis lanceolata) A composition for controlling mushroom flies containing. The composition of claim 1, wherein the caraway plant extract is extracted from caraway seeds. The composition of claim 1, wherein the cinnamon plant extract is extracted from cinnamon bark or leaves. The composition of claim 1, wherein the Clove plant extract is extracted from a clove bud or leaf. The composition for controlling mushroom flies according to any one of claims 1 to 4, wherein the plant extract is a vegetable essential oil. The method according to any one of claims 1 to 4, wherein the mushroom fly is a Camptomyia spp .) or mushroom mushroom fly ( Lycoriella ingenua ) composition. The composition of claim 6, wherein the mushroom fly is a larva. A method of controlling mushroom flies, comprising using the composition for controlling mushroom flies of claim 1 to crops. The method of claim 8, wherein the mushroom fly is a mushroom fly or long beard mushroom fly. The method of claim 9, wherein the mushroom fly is a larva.

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