KR20110006400A - Insecticidal composition comprising extracts of asarum sieboldii - Google Patents

Abstract

PURPOSE: An insecticidal composition containing Asarum sieboldii extract is provided to efficiently prevent pest such as mite, delphacidae and moth. CONSTITUTION: An insecticidal composition contains Asarum sieboldii or Asarum maculatum extract. The extract is obtained from leaves, stems or roots of Asarum sieboldii using methanol, ethanol, acetone, ethyl acetate, ether, dichloro methane, or hexane. A method for preparing the Asarum sieboldii extract comprises: a step of drying and pulverizing Asarum sieboldii; a step of mixing Asarum sieboldii with methanol in a weight ratio of 1 : 10-1: 40 and extracting for 48 hours; a step of filtering and concentrating; and a step of drying to obtain a crude extract.

Description

Insecticidal composition comprising extracts of footwort grass {INSECTICIDAL COMPOSITION COMPRISING EXTRACTS OF ASARUM SIEBOLDII}

The present invention relates to an insecticidal composition comprising an extract of Asarum sieboldii , and more specifically to environmentally friendly pests such as mites, extinct and moths, which are harmful to the productivity and marketability of farm crops. The present invention relates to an insecticidal composition comprising an extract of footwort, which can be controlled with high efficiency, and a preparation method thereof.

As social needs and interests in society increase, the demand for vegetables and fruits increases rapidly, and the use of chemical fertilizers and pesticides is essential for their stable supply. However, the excessive use of chemical fertilizers and pesticides not only caused pollution of cultivated soil and water quality, but also contaminated the cultivation environment in facility cultivation, which threatened the health of the producers. It is causing serious concern.

At present, due to the safety of agricultural products and the concern about sustainable agriculture through the preservation of the soil environment, organic farming is rapidly spreading not only in our farms but also in the world.

Pesticides using plant extracts, which can replace chemical pesticides, are harmless to humans, livestock, fish and shellfish, and many studies have been conducted due to the fact that there is no concern about environmental pollution and ecosystem disturbance. The use of pesticides using plant extracts can reduce the use of fertilizers and pesticides, thereby conserving the soil environment and ensuring long-term productivity, and eliminating the risk of residual pesticides on crops to secure the safety of agricultural products. It is expected to be effective.

Among farmers who grow fruits and vegetables, eco-friendly organic methods that do not use chemical pesticides and chemical fertilizers are being studied.

In particular, agricultural materials related to aphids, which are frequently studied by organic methods, include garlic, green onions, peppers, morning glory, and herbal extracts.However, these methods are insect repellents and insecticides using individual characteristics of plants accumulated by the experiences of farmers. Systematic research between plants and aphids is somewhat inadequate.

Therefore, the present inventors have studied more systematically about pesticidally active plant extracts and a method of producing the same, which can obtain a control effect of mites, anchovies or moths from plants based on such an environmentally friendly organic method.

In particular, the present inventors concentrated their research on plants that can easily obtain the material, and confirmed the applicability as an insecticide to the extract of the foot of the tree, which is a native plant in Korea, which has many mountains.

The present inventors studied various plants to develop an environment-friendly insecticide composition that can control pests that are a headache of farmers growing grains, vegetables, fruit trees, or flowers. It has been found that insecticides are effective against moths and rice planthoppers.

It is an object of the present invention to provide an environment-friendly insecticidal composition which is harmless to humans, livestock, fish and shellfish, and which is free from environmental pollution and disturbance of ecosystems.

It is also an object of the present invention to provide an insecticidal composition comprising an extract of foot footwort.

It is another object of the present invention to dissolve crude foot extract obtained by mixing and extracting the family tree and methanol in a weight ratio of 1: 10 to 1: 40 in methanol, and then treated with ethyl acetate and butanol by adding water and hexane. It is to provide an insecticidal composition, which is obtained by.

Hereinafter, the present invention will be described in detail.

The present invention is a pesticidal composition characterized in that it comprises an extract of Asarum sieboldii or Asarum maculatum .

Hereinafter, the present invention will be described in more detail.

Asarum sieboldii or Asarum maculatum is a perennial vegetation belonging to the Aristolochiaceae family, which grows in the bark of mountainous areas, and its roots are many nodes and slanted sideways and rooted in nodes. It is also called Seshin because of its thin root and very spicy taste. The leaves usually come in two, long sacks, 5-10 cm in the shape of a heart, and have flat edges. Flowers bloom in April purple, with flower beds appearing between the leaves, with one flower running toward the end. The component is about 2-3% essential oil, the main component is 42.40% methyleugenol.

Foot wigs are effective for treating sickness and pain in the face and wind sickness, customs, warming the inside, lowering the nose, treating the stuffy nose, hardening the wall, brightening the eyes, and treating the toothache. In addition, the central nerve suppression and local anesthesia, cold sedation, analgesic, anti-tussive action, etc. In the traditional herbal medicine, Jinhae, analgesic, diuretic, cold, headache, sedation, rheumatism, arthritis. Pedigree paste has a peculiar peppermint flavor, but when heated, the peppermint fragrance disappears.

In the present invention, the footwort extract for obtaining an insecticidal composition can be used for all plants, such as a foot horn or foot horn grass with rat vines, and can use all the leaves, stems, or roots of the footwort.

According to the present invention, first, the dry sample and the organic solvent, preferably methanol, are dried and ground by mixing the foot stilt, and then mixed in a weight ratio of 1: 10 to 1: 40, extracted at room temperature for 48 hours or more, and then the filtrate is filtered. Complete concentration using a vacuum concentrator and dried in a freeze dryer to obtain a crude extract (crude extract).

In the present invention, as an organic solvent for preparing the crude extract of the footwort, methanol, ethanol, acetone, ethyl acetate, ether, dichloromethane, hexane or a mixture thereof may be used, and preferably methanol or ethanol.

Thus prepared crude extract of the foot of the invention according to the present invention can also be used as a liquid, can also be used to powder after drying, diluted in accordance with the intended use.

The crude extract of the foot of a tree obtained by the method of the present invention is dissolved in a trace amount of an organic solvent, preferably methanol, and then suspended by distilled water, and then separated into hexane, ethyl acetate, butanol, and water in a separated filter, and then separated into layers. Collect. Each fractional layer had insecticidal activity individually, and the hexane fraction and ethyl acetate fraction were more superior to the other solvent fractions under the same concentration.

The fractionated layer of the organic solvent obtained from the crude extract of the foot of a bead can be used as a liquid phase, can be concentrated under reduced pressure, dried and powdered, or can be diluted in accordance with the intended use.

As described above, the extract of the organic solvent of the foot of the tree obtained by the method according to the present invention includes various mites ( Tetranycidae ), including moths ( Ptraella xylostella ) and moths and Nilaparvata lugens, including Tetranychus urticae . There are also specific insecticidal activity in the extinct species, including them can be useful as a multi-functional environmentally friendly pesticide that has an environmental preservation and purification action with no toxicity to natural enemies, bees, and livestock.

Attached Figure 1 shows the results of the insecticidal effect bioassay experiments of Chinese cabbage moth of the insecticidal composition comprising the organic solvent extract of the foot of the tree of the present invention, 48 hours after spraying untreated leaf section photograph And the insecticidal effect of the Chinese cabbage moth from the leaf section photograph on the right side of the extract was treated according to the present invention.

In addition, Figure 2 shows the cabbage house assay results for the Chinese cabbage moth of the insecticidal active composition comprising an organic solvent extract of the foot of the tree obtained by the method of the present invention, 7 days after spraying, untreated cabbage package (left photo In the comparison of the cabbage package (right picture) treated with the insecticidal composition of the organic solvent extract of the Aqueous herbaceous leaf extract (right picture), the excellent insecticidal effect on the Chinese cabbage moth can be confirmed.

According to the present invention, by providing an insecticidal active composition using extracts of footwort grass native to the mountainous region occupying most of the country of Korea, while reducing pollution of the agricultural environment by synthetic pesticides and harmless to the human body In addition, it is expected to increase farm incomes by enabling them to produce vegetables and fruit trees.

Hereinafter, the present invention will be described in more detail with reference to Examples.

Example 1 Preparation of Aqueous Radix Extract

The tree roots were purchased at the Chinese herbal medicine market and crushed finely. 100 g of dry grass samples were immersed in 1 L of methanol, and extracted and filtered at room temperature for 48 hours. The filtrate was completely concentrated using a vacuum pressure concentrator and dried in a lyophilizer and used for testing.

The extraction yield was obtained by taking a certain amount of dry sample, extracting it, concentrating with a vacuum concentrator, and freeze-drying to obtain a percentage of the total amount of dry matter used to prepare crude extract. The extraction yield was 9.7%.

Example 2 Spraying Liquid Preparation

After treating each foot concentration extract obtained in Example 1 to each concentration by the following method was added by adding 250ppm of Triton X (triton X) -100 surfactant.

① Preparation method of 3,000 mg / L (100 ml)

Dissolve 0.3 g of crude extract in 5 ml of methanol (MeOH), add 25 µl of Triton X-100, and adjust the total 100 ml with water.

② Preparation method of 2,000 mg / L (100 ml)

Dissolve 0.2 g of crude extract in 5 ml of MeOH, add 25 µl of Triton X-100, and adjust the total 100 ml with water.

③ Preparation method of 1,000 mg / L (100 ml preparation)

Dissolve 0.1 g of crude extract in 5 ml of MeOH, add 25 µl of Triton X-100, and adjust the total 100 ml with water.

④ 500 mg / L preparation method (100 ml preparation)

Dissolve 0.05 g of crude extract in 5 ml of MeOH, add 25 µl of Triton X-100, and adjust the total 100 ml with water.

⑤ Preparation of 250 mg / L (100 ml)

Dissolve 0.025 g of crude extract in 5 ml of MeOH, add 25 µl of Triton X-100, and adjust the total 100 ml with water.

⑥ Preparation of 100 mg / L (100 ml)

Dissolve 0.01 g of crude extract in 5 ml of MeOH, add 25 µl of Triton X-100, and adjust the total 100 ml with water.

Example 3 Chinese cabbage moth ( Plutella xylostella Insecticidal activity against

1) Indoor black

Bioassay of Chinese cabbage myth moth was carried out by the leaf section method using the spray solution of the foot of the foot extract as prepared in Example 2. The cabbage leaf was cut into Φ5 cm, placed in a Petri dish with filter paper soaked in water, and inoculated with 15 larvae of Chinese cabbage moth 3 larvae, and then sprayed with each foot for 10 seconds and dried in a hood for 4 hours. Pesticides were examined after 24 and 48 hours of storage in the nursery. Live death screening considered dead individuals who did not move by contacting the lobe with the tip of a brush (Table 1).

Insecticidal rate = [Insects / (Insects + Insects)] × 100

TABLE 1

Insecticidal Effect of Chinese Cabbage Moths by Indoor Concentration

density
(mg / L) Insecticide Rate (%) 24 hours 48 hours 3,000 100.0 100.0 2,000 95.0 100.0 1,000 83.4 95.0 500 48.4 78.4 250 31.7 63.4 100 41.2 60.7

As shown in Table 1, the extract of the foot of the foot according to the present invention showed a concentration-dependent insecticide control effect on the Chinese cabbage moth (Fig. 1).

2) Pavement Experiment

The packaging test was performed on the cabbage myth moth using the spraying solution of the foot of the foot extract as prepared in Example 2. The Chinese cabbage used in the test was prepared on the packaging of vegetable plastic house in Prayer Agricultural Research Institute on September 16, 2008. When the density was 10 to 15 per week, sprayed with 1000 mg / L of footwort extract, respectively, 3, After 7 days, the control value was investigated and the average value was measured.

Control value (%) = [(untreated worms vs. untreated worms) / untreated worms] x 100

Table 4 shows the results of the insecticidal effect packaging experiments of Chinese cabbage moths on the crude crude extracts of the Chinese cabbage.

TABLE 2

Insecticidal Effect of Chinese Cabbage Extract from Chinese Cabbage

sample
1 time Episode 2 Viability Rate (%) Control is (%) Viability Rate (%) Control is (%) Foot print 15.5 76.3 ± 10.7 35.1 71.2 ± 10.7

As shown in Table 2, the extract of the footfoot grass was also found to have excellent insecticidal activity in the field test for Chinese cabbage moth (Fig. 2).

Example 4 Two-pointed Mite Tetranychus urticae Insecticidal activity against

Bioassay was carried out for the two-spotted mite using the spray solution of the foot of the valley extract prepared as in Example 2. The kidney bean leaf slices cut to 2.5 cm in diameter in a 5.5 cm diameter Petri dish were placed on cotton wool soaked in water and inoculated with 30 adult females with a fine brush. Each of the prepared spraying solution of the footwort extract by concentration was sprayed with a small spray so that the leaf surface was sufficiently wet and dried. As described above, the insecticidal rate after 24 and 48 hours was investigated while being stored at 25 ° C. and 60% relative humidity after treatment (Table 3).

[Table 3]

Insecticidal Effect of Footwort Extract on Two-spotted Mites

density
(mg / L) Spotted Mite ( Tetranychus urticae ) 24 hours 48 hours 3,000 70 80 1,000 60 63 333 37 40 111 0 0 37 0 0

As shown in Table 4, the organic solvent extracts of the footwort grass showed high insecticidal activity against two-spotted mite and rice planthopper.

Example 5 rice bulbs Nilaparvata lugens Insecticidal activity against

Insecticidal activity against rice bran was investigated using the spraying solution of the footwort extract prepared as in Example 2. After seeding, root portions of five Dongjin rice seedlings 7 to 8 days old were wound with cotton wool and inserted into the bottom portion of a glass test tube having a diameter of 3 cm x 15 cm. Twenty three larvae of the larvae were harvested from a breeding box using a diaphragm pump equipped with a shock absorber and placed in a test tube containing rice seedlings. Each spraying solution of the prepared foot extracts of each concentration was sprayed directly from the entrance of the test tube toward the rice seedlings attached to the rice seedlings with a fine sprayer. After treatment, the entrance of the test tube was covered with nylon mesh to prevent the escape of the rice hopper.

After inoculation, the cells were stored at 25 ° C. and 60% relative humidity, and abnormal growth and insecticidal rates were examined 24 and 48 hours after chemical treatment (Table 4).

[Table 4]

Insecticidal Effects of Footwort Extracts on Rice Anthurium

density
(mg / L) Nilaparvata lugens 24 hours 48 hours 3,000 100 100 1,000 90 95 333 65 70 111 15 20 37 0 0

Example 6 Test of the Control Effect of Chinese Cabbage Moths by Foot Solvent Extract

75 g of the crude extract extracted with methanol was repeatedly dissolved in 25 g of three times, dissolved in a minimum amount of methanol, suspended in distilled water, and then fractionated in a separatory solution, and concentrated under reduced pressure. For each of the solvent fractions obtained, the effect of controlling Chinese cabbage moths was tested by the indoor assay method of Example 3, and the results are shown in Table 5 below.

TABLE 5

Control Effect of Chinese Cabbage Extract by Solvent Fraction

density
(mg / L) Fractional layer Control price (%) Day 3 Day 7 1,000 Hexane
Ethyl acetate
Butanol
water 100.0
100.0
93.3
6.7 93.3
93.3
87.1
12.4 500 Hexane
Ethyl acetate
Butanol
water 80.9
75.1
69.3
5.8 80.9
80.9
69.3
5.8 250 Hexane
Ethyl acetate
Butanol
water 83.3
56.7
36.7
13.3 83.3
60.0
36.7
13.3

♩ Test Title: Crossed Chinese Cabbage

As shown in Table 5, the insecticidal effect of Chinese cabbage moth was higher in the hexane fractionation layer and the ethyl acetate fractionation layer than in the other solvent layers.

The above embodiments, test examples and drawings are merely for the purpose of describing the contents of the invention in detail, and are not intended to limit the scope of the technical idea of the invention, and the present invention described above is usually in the technical field to which the present invention belongs. As those skilled in the art, various substitutions, modifications and changes are possible within the scope without departing from the spirit of the present invention is not limited to the embodiments and the accompanying drawings, as well as the claims described below It should be determined that the claims are included in their equality.

Figure 1 shows the results of the insecticidal effect bioassay experiment of Chinese cabbage moth of the insecticidal active composition comprising an organic solvent extract of the foot of a tree is obtained by the method of the present invention, the left picture is an untreated leaf section, the right picture is seen It is a photograph showing the leaf section 48 hours after the foot extract of the invention extract treated with organic solvents.

Figure 2 shows the results of the cabbage house assay for the Chinese cabbage moth of the insecticidal active composition comprising an organic solvent extract of the foot of the tree of the invention, the untreated cabbage package (left photo) and the foot of the organic solvent Photograph showing the insecticidal effect of Chinese cabbage moth after 7 days of spraying cabbage package (right picture) treated with the insecticidal composition of the extract.

Claims (3)

An insecticidal composition comprising an extract of Asarum sieboldii or Asarum maculatum . The insecticidal composition according to claim 1, wherein the extract is an extract using at least one solvent selected from the group consisting of methanol, ethanol, acetone, ethyl acetate, ether, dichloromethane and hexane. 3. The insecticidal composition according to claim 1 or 2, wherein the pests of interest are mites, moths and extinct species.

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