KR20120060585A - Composition for Controling Lycorma delicatula, the Methods of Preparing it and the Method of Controling Lycorma delicatula Using it - Google Patents

Abstract

PURPOSE: A Lycorma delicatula repellent composition containing natural extract from plants is provided to replace agrochemical. CONSTITUTION: A Lycorma delicatula repellent composition contains 100 weight parts of Melia azedarach alcohol extract, 200-600 weight parts of Sophorae japonica alcohol extract, and 200-600 weight parts of Chenopodium alcohol extract. The alcohol extract is prepared at room temperature using 90-99% ethanol for 40-60 hours. The repellent composition further contains 5-15% ethanol solution and surfactant.

Description

Composition for controlling cicada and its preparation method and control method using same {Composition for Controling Lycorma delicatula, the Methods of Preparing it and the Method of Controlling Lycorma delicatula Using it}

 TECHNICAL FIELD The present invention relates to a cicada control agent, and more particularly, to a cicada control agent comprising a natural extract from a plant as an active ingredient.

The cicada (Lycorma delicatula) is a pest belonging to the group of the insect cicada cicada, and is 14-15 mm long and 40-50 mm long. The body color is light brown and the back of the chest and especially the belly are often dark brown. The first reported cicada in Korea by Doi in 1932 is reported to be a subtropical insect that lives in relatively high temperature areas such as China and Southeast Asia. Flower cicada is not well known to people only a few years ago, and this has not been a problem in Korea. However, in 2006, it began to attract people's attention as it occurred in Seoul and Gyeonggi-do, and it appears to have settled in China, and since then, it has been increasing nationwide as the density increases year by year. Flower cicada not only disgusts people because many individuals live in herds, but also sucks plants and excretes them to inhibit photosynthesis or cause sooty disease and, if severe, kill them. 41 species, including 38 woods and 3 herbaceous plants, including leather, oak, pine, American ivy, buckthorn, and vine, are known as host plants of cicada.

Although chemical pesticides have been used mainly for controlling cicada, chemical pesticides cause soil acidification and pollution problems, toxicity to human beings, environmental problems due to pesticide residues, etc. Recently, due to increasing awareness of environmental problems and consumers' preference for eco-friendly foods, there is a growing interest in eco-friendly agricultural materials that can replace chemical pesticides. Eco-friendly agricultural materials that can replace chemical pesticides include the use of natural product pesticides extracted from plants and the use of biological control factors utilizing natural enemies. In most cases, the control of flower cicada using natural extracts is often the level of folk remedies for individual farmers, whose effectiveness has not been objectively verified. In addition, there are some products that have been commercialized by some manufacturers, but there are still many problems to be solved before they can be commercialized by replacing chemical pesticides due to limitations in control effects, difficulty in manufacturing method, and limited shelf life. . In particular, in the case of a product having a poor control effect, it may cause a secondary problem caused by an increase in natural populations, and thus may not be a preferable control method.

Attempts have been made to use a mixture of other insecticides such as Sophorae japonica, Chenopodium, or Melia azedarach as a natural plant ingredient with insecticidal effects, but most of them have unclear effects. Chemical pesticides could not be used as pesticides.

One aspect of the present invention is to provide a new cicada control composition composed by effectively extracting the active ingredient from the painting tree, tusks and mulberry tree.

Another aspect of the present invention is to provide a method for producing a cicada control agent comprising a method of effectively extracting the active ingredient from the painting tree, tusks and mulberry tree so as to have a sufficient cicada control effect.

Another aspect of the present invention is to provide a cicada control method using the cicada control composition.

Other objects and advantages of the present invention will be described below and will be better understood by practice of the present invention.

In the present invention,

100 parts by weight of mulberry tree alcohol extract at room temperature; 200-600 parts by weight of alcohol extract room temperature extract; And the cicada jujube 200-600 parts by weight of the cicada control composition comprising an alcohol room temperature extract is provided. The mulberry tree; Painting trees; And alcoholic room temperature extract of Myeongju is extracted 40-60 hours in a dark state at room temperature. Extraction may be carried out either before or after mixing mulberry, sapling and tusks, in which case the resulting extracts are mixed without mixing. Preferably, the alcohol is 90% to 99% ethanol. Also preferably, the mulberry tree; Painting trees; And alcohol at room temperature extract of Myeongju is concentrated under reduced pressure at 30 ~ 43 ℃ after alcohol extraction, and preferably lyophilized after concentration under reduced pressure. The cicada control composition of the present invention, in the preferred embodiment further comprises an aqueous solution of 5-15% ethanol in an amount capable of diluting the entire alcoholic room temperature extract of the mulberry tree, painting tree and myeongju 100 to 2,000 times It may further include a surfactant that serves as an electrodeposition agent.

In the present invention,

(a) 100 parts by weight of mulberry; 200-600 parts by weight of painting wood; And 200 to 600 parts by weight of the mixture, or each of them in a 5 to 15 volume of alcohol to extract 40 to 60 hours in the dark at room temperature,

(b) mulberry trees; Painting trees; And mixing the tusks before the extraction, or mixing each of these extracts after the extraction, and

(c) mulberry tree obtained through (a) and (b); Painting trees; And it provides a method of producing a cicada control agent comprising the step of concentrating the reduced extract at 30-43 ° C mixed extract of mingled wine. In the extracting step, 90% to 99% ethanol is preferably used as the alcohol and extracted for at least 48 hours. In a preferred embodiment further comprises the step of lyophilizing the concentrated extract obtained following the step of concentration under reduced pressure.

In the present invention,

The cicada control composition is diluted to 100 to 2,000 times in 5 to 15% ethanol aqueous solution is provided with a cicada control method comprising spraying on the plant. Preferably, the cicada control composition is diluted with an ethanol aqueous solution and then sprayed by adding 0.1 to 2 wt% of a surfactant that serves as an electrodeposition agent.

The calyx control composition obtained in accordance with the present invention, as confirmed through the examples, since the control alone exhibits excellent calendula control activity of more than 80%, it is effective as an alternative to the chemical pesticides used in the past Can be used. The cicada control composition of the present invention can solve the soil pollution, toxicity problems to the human body and can be effectively used for cicada that has been resistant to chemical pesticides. In addition, the composition of the present invention can be combined with or combined with other insecticides as it has a pronounced insecticidal effect.

Sophorae japonica has alkaloids throughout the plant, matrin C ₁₅ H ₂₄ ON ₂ , cytigene C ₁₁ H ₁₄ ON ₂ , N-methylcytizin C ₁₂ H ₁₆ ON ₂ , sopocarpine C ₁₅ H ₂₄ It contains components such as ON ₂ H ₂ O. The painting extract has been spotlighted as a raw material for organic agriculture because of its high insecticidal action and low toxicity. The main components of Chenopodium include geraniol, alpha-pinene, aritasone, ascaridole and butyric-acid. Melia azedarach is a deciduous broad-leaved arboreous tree in tropical or subtropical regions of Southeast Asia. It has been reported to have the effect of avoidance, feeding, insecticide and growth inhibition. Azadirachtin and eugenol, which are included in the composition, have a high insecticidal effect and low toxicity, and thus are widely used as raw materials for organic farming. Painting tree, hyacinth tree, and mulberry tree were each added to other insecticides and used for the purpose of enhancing insecticidal action, but the effect was not obvious. The present invention effectively extracts the active ingredient from these three plants and provides a cicada control composition of a new composition using this extract.

In order to obtain an insecticidal extract from these plants, alcohol extraction after hot water extraction, alcohol extraction while heating to a constant temperature, or heating and concentration after alcohol extraction have been generally used. However, the present inventors have systematically studied the painting tree, mallow, mulberry tree, and concluded that it is necessary to extract alcohol at room temperature for a long time without heating in order to obtain an extract with high insecticidal activity. To this end, in the present invention, the painting tree, myeongju, mulberry tree is extracted with alcohol at room temperature for a long time, preferably 40-60 hours at room temperature in the dark state. Preferably the alcohol is 90-99% ethanol, particularly preferably 95% or more ethanol, and placed in 5 to 15 times the volume of ethanol and extracted for at least 48 hours. Preferably, the extract is separated by filtration with filter paper.

Prior to extraction, the painting tree, myeongju, mulberry wood is preferably dried to less than 5% moisture content and then pulverized to about 1-2mm. Painting, tusks and mulberry trees are mixed before extraction or after each extraction. The blending ratio of mulberry, sapling, and nectar is 100 parts by weight of mulberry, 200-600 parts by weight and 200-600 parts by weight, based on the raw materials or powder before extraction. More preferably, 300 to 500 parts by weight of each painting tree and quinoa are mixed based on 100 parts by weight of mulberry.

Preferably, these extracts are concentrated completely under reduced pressure at 30 to 43 ° C. after the alcohol extraction as described above. Also preferably lyophilized after concentration under reduced pressure. Insecticidal activity is reduced when the treatment at a high temperature of 43 ℃ or higher, at this time the temperature should not exceed 43 ℃. More preferably, it does not exceed 40 degreeC. The concentrate thus obtained is preferably lyophilized using a lyophilizer. Lyophilization can prevent loss of the active ingredient over time. The calyx control composition of the present invention thus obtained has a high control effect on the cicada as confirmed through the examples.

The cicada control composition of the present invention comprising a painting tree, a hyacinth, a mulberry tree extract is diluted 100-2,000 times in a 5-15% ethanol aqueous solution and then sprayed on a plant. More preferably, it is diluted in 10% aqueous ethanol solution. In addition, preferably, 0.1 to 2% by weight of a surfactant, which serves as an electrodeposition agent, is evenly sprayed on the entire leaf surface of the plant. As the surfactant, a nonionic surfactant is preferable. For example, TWEEN-80 (PEO (20) -Sorbitan Monooleate), TWEEN-60 (PEO (20) -Sorbitan Monostearate), etc., which have been confirmed to be safe to the human body, may be used. Can be used.

In addition, the composition of the present invention, known to be known for its insecticidal effect, such as betel nut extract with arecoline (arecoline) or pyrethrin (pyrethrins) as an active ingredient in order to enhance the insecticidal effect or to expand the pesticide range The plant extract of may be further included. In addition, the painting tree, mallow, mulberry tree extract of the present invention can be used as a compound insecticide in combination with other insecticide components.

Hereinafter, the present invention will be described in more detail with reference to specific examples and test examples. However, these examples are only for illustrating the present invention in more detail, the scope of the present invention is not limited by these examples.

Example 1

Preparation of Cicada Control Composition

(1) The collected sapling, mallow and mulberry trees were dried to 5% or less of water content, and then ground to a size of 1 to 2 mm.

(2) 40 g of crushed sapling tree, 40 g of mallow perilla, and 10 g of mulberry tree were added to 1,000 ml of 95% ethanol, and extracted at room temperature for 48 hours.

(3) The obtained extract was filtered through filter paper and concentrated completely at 40 ° C. using a vacuum concentrator.

(4) The concentrated extract was dried in a lyophilizer to obtain a flower cicada control composition of the present invention.

Test Example 1

Flower Cicada Control Effect

(1) The cicada control composition prepared in Example 1 was diluted 1,000-fold in a 10% aqueous ethanol solution to prepare a diluent to be sprayed.

(2) When some of the cicada ( Lycorma delicatula ) was formed in the packaging of the facility, the pre-density was examined and the dilution was applied and the control value was examined after 3 and 7 days. The test was carried out in the vineyard constructed according to the general practice. The test packaging was not sprayed with other pesticides at all before and after the test period, and the planting distance was 75 ㅧ 40cm. 1,3,5 spheres were treated untreated with 6 test spheres, and 2,4,6 spheres were tested in three repetitions by treating 1,000-fold dilutions. The area per each test port was 20 m ² . Table 1 shows the results of day 3 after drug treatment, and Table 2 shows the results of day 7 after drug treatment.

-Exam period: June 2010

-Test Place: Cheongju, Chungbuk

-Arrangement and area of test zone: 3 times of random placement method

-Target pests: Lycorma delicatula

-Test crops (varieties): grapes (campbell early) / 11 years

Seedling overview: It was conducted in vineyards constructed according to general practice, and no other pesticides were sprayed before or after the test period.

-Pest incidence: The average density of untreated plots was 234.0, which was sufficient for drug efficacy review.

Test Example  2

Weakness test

Dilutions were made in the same manner as in Test Example 1, but two dilutions were prepared: 1,000-fold dilutions and 500-fold dilutions. A weak test was performed using a 1,000-fold dilution as a reference amount and a 500-fold dilution as a double amount. The test sphere was made in the same manner as in Test Example 1, but 8 balls were made, 1, 4, 7 balls were treated without treatment, 2, 5, 8 balls were treated with 1,000-fold dilution, and 3, 6, 9 balls were treated with 500-fold dilution. It was. Three replicates of each test were used. When there was no degree of weakness at all, it was set as "0", and when there existed, it divided into five steps of "1-5", and compared with the non-processing tool. Evaluation was carried out 3, 5, 7 days after drug treatment. The survey method was to examine whether there was any apparent weakness in the standard dose and the volume. As a result, in all nine test groups, no apparent damage was observed in the standard dose and the same amount as the untreated group.

The cicada control composition of the present invention exhibits excellent cicada control activity, and can be used as an effective cicada control agent in place of the chemical pesticides that have been used. The flower cicada control composition of the present invention can be used as an environmentally friendly control agent that can solve the soil pollution, toxicity problems to the human body, it can also be used in the flower cicada that has shown resistance to chemical pesticides. In addition, it can also be used by the method of combining or using with other pesticides.

Claims (12)

100 parts by weight of mulberry tree alcohol extract at room temperature; 200-600 parts by weight of alcohol extract room temperature extract; And Mica wine 200-600 parts by weight of the room temperature extract of the cicada control composition. According to claim 1, The mulberry tree; Painting trees; And ethanol extract at room temperature of the flower cicada control composition, characterized in that extracted at room temperature for 40 to 60 hours. The method of claim 2, wherein the alcohol is 90% to 99% ethanol control composition characterized in that the ethanol. According to claim 1, The mulberry tree; Painting trees; And ethanol extract at room temperature of the flower cicada control composition, characterized in that concentrated under reduced pressure at 30 ~ 43 ℃ after alcohol extraction. 5. The cicada control composition according to claim 4, which is freeze-dried following the reduced pressure concentration. According to claim 5, The mulberry tree; Painting trees; And 5-15% ethanol aqueous solution in an amount capable of diluting the whole alcoholic extract of Myeong juk 100 times to 2,000 times. The cicada control composition according to any one of claims 1 to 6, further comprising a surfactant serving as an electrodeposition agent. (a) 100 parts by weight of mulberry; 200-600 parts by weight of painting wood; And 200 to 600 parts by weight of the mixture, or each of them in a 5 to 15 volume of alcohol to extract 40 to 60 hours in the dark at room temperature,
(b) mulberry trees; Painting trees; And mixing the tusks before the extraction, or mixing each of these extracts after the extraction, and
(c) mulberry tree obtained through (a) and (b); Painting trees; And Method of producing a cicada control agent comprising the step of condensation under reduced pressure at 30 ~ 43 ℃ mixed extract of mingled wine. The method of claim 8, wherein the extraction step using a 90% to 99% ethanol as alcohol extraction method of calyx control, characterized in that the extraction for more than 48 hours. The method of claim 8, further comprising lyophilizing the concentrated extract obtained after the step of concentration under reduced pressure. The method of controlling cicadas comprising diluting the calyx control composition of claim 1 to 100 to 2,000 times in an aqueous 5 to 15% ethanol solution. The method of claim 11, wherein after diluting the calyx control composition in an ethanol aqueous solution and spraying by adding 0.1 to 2% by weight of a surfactant that acts as an electrodeposition agent.