KR102099825B1 - Compositions for controlling poultry red mite comprising extracts derived from natural materials and preparation method of the same - Google Patents

Abstract

The present invention relates to a veterinary composition for controlling chicken mites, an active ingredient comprising a clove extract; And including a surfactant containing glycol, it is possible to further improve the stability of the veterinary composition, ease of spraying, insecticidal efficiency, and the like.

Description

Composition for the control of chicken mite (wagumo) using natural products and its manufacturing method {COMPOSITIONS FOR CONTROLLING POULTRY RED MITE COMPRISING EXTRACTS DERIVED FROM NATURAL MATERIALS AND PREPARATION METHOD OF THE SAME}

The present invention relates to a composition for controlling chicken mites (wagumo) using natural products and a method for manufacturing the same.

The most common species of poultry farms are chicken mites (fowl red mite, Dermanyssus gallinae) and chicken fowl (northern fowl mite, Ornithonyssus sylviarum).

These chicken mites are distributed not only in Korea but also around the world, and have a habit of parasitizing and sucking blood. In particular, wagumo parasitizes the host chicken, causing itching and anemia, and can even lead to death in severe cases. This mite, including feed, incubates eggs in the cracks of the house, and exhibits the characteristic of moving to the host only when vampires are needed, so it is very difficult to control.

Chicken red mange is one of the most important external parasites that cause spawning problems in Korea and other countries that are poultry farming. This species is found in almost all birds, including poultry, leafy birds, and wild birds. It is a very annoying person for people working in poultry because it sometimes sucks mammals, including humans, and causes skin diseases. In addition, the parasite acts as a mediator for viruses that cause various diseases related to chickens, such as poultry typhoid and poultry cholera.

It is known that more than 35 types of materials are used to control chicken mites, some of which have excellent effects, but are inappropriate for use in food safety and environmental aspects. In addition, repeated and long-term use of drugs has led to the development of resistant mites, and no clear alternative drugs have been developed to replace them.

On the other hand, in recent years, there has been a problem of mass destruction of poultry due to avian influenza (AI), encapsulated hepatitis, and infectious bronchitis, and a decrease in productivity. Consumer complaints are expressed due to the use of indiscriminate antibiotics that have been applied for the past, and there is a growing demand for control drugs for efficient and healthy poultry breeding while ensuring food stability.

In addition, in recent years, the awareness of safe food has been increased, and there is a growing concern about the possibility of antibiotics or pesticides remaining in poultry meat or poultry eggs, and the market demand to refrain from using chemicals of pesticide ingredients such as pesticides is increasing. . Therefore, it is necessary to develop a technology that can relieve chicken mites (wagumo) in an eco-friendly and safe way.

Domestic registration patent No. 10-1505018, announcement date March 23, 2015, pesticide removal method and pesticide prepared thereby Domestic Patent Publication No. 10-2013-0142588, Publication Date December 30, 2013, Insecticidal Composition Containing Insecticidal Essential Oils and / or Plant Additives

It is an object of the present invention to provide a veterinary composition for controlling chicken mites and a method for controlling chicken mites using the same, which contains a natural extract as an active ingredient and has improved stability and control efficiency.

In order to achieve the above object, the veterinary composition for controlling chicken mites according to an embodiment of the present invention includes an active ingredient comprising a clove extract; And a surfactant comprising glycol, wherein the active ingredient further comprises any one selected from the group consisting of castor oil, cinnamon oil, and combinations thereof, and the surfactant is polyoxyethylene dodecyl monoether, sodium. Bis (2-ethylhexyl) sulfosuccinate and any one selected from the group consisting of a combination thereof further comprising, 5 to 85% by weight of the active ingredient, based on the entire veterinary composition for controlling the chicken mite, the The surfactant contains 15 to 55% by weight and the remaining amount of sterile water or ethanol, and the glycol comprises 10% by weight or more.

The glycol may be any one selected from the group consisting of polyethylene glycol, propylene glycol, and combinations thereof.

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The surfactant may include the glycol and sodium bis (2-ethylhexyl) sulfosuccinate in a weight ratio of 1: 0.2 to 1.6.

The surfactant may include the glycol and polyoxyethylene dodecyl monoether in a weight ratio of 1: 0.1 to 1.4.

The active ingredient may include the clove extract and castor oil in a weight ratio of 1: 0.5 to 6.

The active ingredient may include the clove extract and cinnamon oil in a weight ratio of 1: 0.4 to 5.

The active ingredient may include 1 to 5 parts by weight of the castor oil, and 1 to 4 parts by weight of the cinnamon oil based on 1 part by weight of the clove extract.

The veterinary composition for remedy of chicken mite may be a liquid formulation applied by spraying the cage and surrounding the cage.

A method for controlling chicken mite in a poultry farm using a veterinary composition for controlling chicken mites according to another embodiment of the present invention includes: a manufacturing process of diluting a veterinary composition for the relief of chicken mites described above in water; And a control process of spraying the administration solution around the cage and around the cage.

The dilution may be that the veterinary composition for controlling chicken mite and the water are mixed in a weight ratio of 1: 150 to 1,000.

The control process may be performed 2 to 4 times with an interval of 5 to 10 days.

When describing the present invention in the present specification, no distinction is made between “singular” and “plural” of nouns, and singular expression is used as a concept including singular and plural, unless it is clear that the singular only means singular in context. do.

“Or”, “and / or” are used interchangeably, unless stated otherwise, and the description of “A or B” or “A and / or B” means “A, B, or A and B”. It can be interpreted as meaning including meaning.

Hereinafter, the present invention will be described in more detail.

The inventors of the present invention, chicken mites (wagmo, red chicken mite, Dermanyssus) that cause particularly serious damage to poultry farms, such as reduced productivity of laying hens gallinae ) while effectively controlling the effects of these remedies on use as foods such as poultry meat and eggs, that is, securing two food safety problems simultaneously, while studying various methods, using natural extracts is effective remedy It was possible to ensure food stability, and it was confirmed that it is possible to remedy in an environmentally friendly manner with a high stability formulation and completed the present invention.

In order to achieve the above object, the veterinary composition for controlling chicken mites according to an embodiment of the present invention includes an active ingredient comprising a clove extract; And surfactants.

The active ingredient may further include any one selected from the group consisting of castor oil, cinnamon oil, and combinations thereof.

Clove extract, Clove ( Eugeniacaryophyllata As an extract of thumb.), one produced by the process of manufacturing a natural product extract described below using leaves, stems, roots, flowers, seeds, or outposts can be applied. Clove is a tropical evergreen tree, native to the Molucca Islands and is produced in Zanzibar Island in Tanzania, Sumatra in Indonesia, Brazil, Malaysia, Philippines, Vietnam and southern China. The bud is said to be cloves and the dried flower is said to be the mother cloves. The buds look like nails and have a scent, so they are called clove (丁香, clove). It is used for edible or medicinal purposes.

The cloves extract may be any one selected from the group consisting of cloves leaf extract, cloves flower extract, cloves bud extract, cloves stem bark extract, and combinations thereof, alone or in combination with other natural extracts When used, it shows strong chicken mite remedies.

Castor oil, castor ( Ricinus communis Linne , which refers to the seed oil of the Euphorbiaceae and Euphorbiaceae. Castors are annual plants of the Euphorbiaceae and the genus Ricinus. It is cultivated all over the country, and in August-September, light yellow or light red-sak flowers bloom at the end of the main stem, and the oval-shaped, flat, dark-brown spotted castor is opened. This castor oil is also used as industrial lubricants and is used as a medicine in oriental medicine.

Cinnamon oil refers to an oil extracted from cinnamon, a medicine made from the bark of the evergreen tree (天 竹 桂, Cinnamomum japonicum SIEB.), An evergreen tree belonging to the camphoraceae family.

The above active ingredients may be applied to the veterinary composition for controlling chicken mite, including i) clove extract alone or ii) castor oil, cinnamon oil, or both together with the clove extract.

All of the above active ingredients are substances that have been experimentally confirmed as a natural mite remedy effect.

For the purpose of improving the stability, convenience, insecticidal rate, etc. of the active ingredients, a surfactant containing glycol is applied as an excipient in the present invention.

As a result of applying various types of surfactants as components of a veterinary composition for controlling chicken mites together with the above-mentioned active ingredients, it was experimentally confirmed that the effect of controlling chicken mites depends on the type of surfactant, especially glycols. It was confirmed that the effect was excellent.

In this case, the glycol may be any one selected from the group consisting of polyethylene glycol, propylene glycol, and combinations thereof. When glycol is applied together with the active ingredient as the surfactant, the effect of controlling chicken mite can be further improved.

The surfactant may further include any one selected from the group consisting of polyoxyethylene dodecyl monoether, sodium bis (2-ethylhexyl) sulfosuccinate, and combinations thereof. Polyoxyethylene dodecyl monoether was also experimentally confirmed to improve the stability of the active ingredient derived from the natural product, and sodium bis (2-ethylhexyl) sulfosuccinate was also experimentally confirmed to have such stability enhancing effect. When used as a surfactant together with the above glycols, the effect of controlling chicken mite by the composition of the present invention may be further improved.

The veterinary composition for controlling chicken mites may include 5 to 85% by weight of the active ingredient and 15 to 55% by weight based on the entire veterinary composition for controlling chicken mites. The remaining amount contains sterile water or ethanol. Specifically, the veterinary composition for controlling chicken mites may include 10 to 80% by weight of the active ingredient, 20 to 60% by weight of the surfactant, and a residual amount of sterile water or ethanol. More specifically, the veterinary composition for controlling chicken mites may include 20 to 50% by weight of the active ingredient, 20 to 50% by weight of the surfactant, and the remaining amount of sterilized water. When the active ingredient and the surfactant are applied in such a content, the application amount of the active ingredient can be minimized while maximizing the insecticidal and repellent effects of chicken mite.

When the mixture of the cloves extract and castor oil is used as the active ingredient, the cloves extract and castor oil may be included in a weight ratio of 1: 0.5 to 6, and the cloves extract and castor oil may be 1: 2 to 5.5. It can be included in a weight ratio of. When the mixture of clove extract and castor oil is applied at a ratio of such a content, the effect of controlling chicken mite can be further improved.

When the clove extract and cinnamon oil are mixed and applied as the active ingredient, the clove extract and cinnamon oil may be included in a weight ratio of 1: 0.4 to 5, and may be included in a weight ratio of 1: 0.8 to 2.8. , 1: may be included in a weight ratio of 1.5 to 2.6. When a mixture of clove extract and cinnamon oil is applied at a ratio of such a content, the effect of controlling chicken mite can be further improved.

When the clove extract, castor oil, and cinnamon oil are mixed and applied as the active ingredient, the active ingredient is 1 to 5 parts by weight of the castor oil based on 1 part by weight of the clove extract, and 1 to cinnamon oil It may include 4 parts by weight, and may include 2.5 to 5 parts by weight of the castor oil, and 1 to 3 parts by weight of the cinnamon oil based on 1 part by weight of the clove extract. When the active ingredients are applied together at a ratio of such a content, the persistent remedy effect on chicken mite can be further improved.

The surfactant may also be applied alone as glycols, but polyoxyethylene dodecyl monoether and sodium bis (2-ethylhexyl) for the purpose of improving stability, improving convenience, improving insecticidal efficiency, and increasing the shelf life of the formulation. Sulfosuccinate and combinations thereof may be applied by mixing in a constant ratio.

Specifically, the surfactant may include the glycol and sodium bis (2-ethylhexyl) sulfosuccinate in a weight ratio of 1: 0.2 to 1.6. In this case, there is an advantage that the surface tension of the formulation is increased, the insecticidal rate is further improved, and the shelf life of the formulation is increased. The surfactant may include the glycol and polyoxyethylene dodecyl monoether in a weight ratio of 1: 0.1 to 1.4. In this case, the surface tension of the formulation may be increased and the insecticidal rate may be improved by adding a surfactant.

The surfactant may include the glycol, the polyoxyethylene dodecyl monoether, and the sodium bis (2-ethylhexyl) sulfosuccinate in a weight ratio of 1: 0.1 to 1.4: 0.2 to 1.6. As a result, when the surfactant is applied together with the active ingredient, effects such as increased stability, improved convenience, improved insecticidal efficiency, and increased shelf life can be obtained.

More specifically, the surfactant is 0.2 to 0.9 parts by weight of polyoxyethylene dodecyl monoether based on 1 part by weight of the glycol, and sodium bis (2-ethylhexyl) sulfosuccinate in 0.3 to 1.1 parts by weight. In this case, while further improving the insecticidal rate, it is possible to obtain an effect of increasing the shelf life of the formulation.

The veterinary composition for remedy of chicken mites may be a formulation of a liquid formulation applied by spraying the cage and surrounding the cage. These formulations can be easily applied to cage-type cages, and can safely obtain chicken mite relief effects even after repeated use.

The veterinary composition for controlling chicken mites, in addition to the above-mentioned, may further include additives such as a veterinary acceptable carrier or excipient. In addition, the veterinary composition may be administered alone or in combination with a carrier, excipients, and the like, and such a dosage form may be a dosage form for a single administration or multiple administrations.

The liquid formulation can be prepared by adding a suitable colorant, flavoring agent, stabilizer, viscous agent, etc., and colorants, flavoring agent, stabilizer, viscous agent, etc. are usually applied within a range that does not inhibit the activity of the active ingredient Ingredients and content may be included in the veterinary composition for controlling the chicken mite.

The veterinary composition for remedy of chicken mite of the present invention can be applied and treated as much as the permissible amount per individual of poultry, and can be sprayed by applying, spraying, etc. The dosage and the number of times of administration can be adjusted and applied. Specifically, the concentration of the active ingredient in the poultry rearing area can be applied at a concentration of 5000 ppm or less at one application, can be applied at a concentration of 2000 ppm or less, can be applied at a concentration of 1000 ppm, or a concentration of 500 ppm or less Can be sprayed.

However, the application amount may vary depending on the content of the active ingredient contained in the veterinary composition for controlling chicken mites, the condition of the individual (body weight, etc.), the severity of the chicken mite infection condition, and the like. If necessary, the total daily dose may be divided into several times, and may be divided into several times during the day.

The method for preparing the veterinary composition for controlling chicken mites can be prepared by mixing the active ingredient extract or oil and the composition of the surfactant, and can be applied if it is a method commonly used in the preparation of a veterinary composition. , Is not limited to a particular method.

A method for controlling chicken mites in a poultry farm using a veterinary composition for controlling chicken mites according to another embodiment of the present invention includes: a manufacturing process of preparing a dosing solution by diluting the veterinary composition for controlling chicken mites described above in water; And a control process of spraying the administration solution around the cage and around the cage.

The dilution may be that the veterinary composition for controlling chicken mite and the water are mixed in a weight ratio of 1: 150 to 1,000, and the control process may be performed 2 to 4 times with an interval of 5 to 10 days. have.

The detailed description of the veterinary composition for remedy of chicken mites is omitted because it overlaps with the above description.

When this method is applied, it is possible to proceed with eco-friendly and safe chicken mite remediation using natural substance-derived materials, and the remedy is also excellent.

The veterinary composition for the control of chicken mite of the present invention and its manufacturing method utilize natural extracts as an active ingredient, but have high stability in a stable formulation and show excellent effect in controlling chicken mite even at low concentrations. It also has some minor advantages.

1 is a photograph of a chicken mite (wagumo) taken in the course of an experiment of an embodiment of the present invention.
Figure 2 is a graph showing the results of an outdoor clinical insecticidal effect of the composition for controlling chicken mites conducted in an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.

Chicken mite  Methods for testing remedies

Filter paper NO.2 was spread on an insect petri dish, 3 ml of sterile water was dispensed, and chicken mites ( Dermanyssus gallinae ) were inoculated. Each of the test drugs was spray-sprayed to each of the fed ticks inoculated with chicken mites at the concentrations listed in the table, and cultured at room temperature for 24 hours and 48 hours. After 24 hours and 48 hours elapsed, it was observed under a microscope to confirm whether chicken mites were killed or not, and the results are shown in the respective tables below.

The above experiment was independently conducted at least three times using the same composition sample.

Chicken mite  Selection of active ingredients with remedies

1) Preparation of natural extracts

Natural materials such as cloves, cinnamon and grapefruit seeds were each washed, dried and powdered to prepare natural powders such as cloves and cinnamon powder. Each of the natural product powder was mixed with 100 parts by weight and 30 parts by weight of hot water to prepare a mixture, and extraction was performed, and extraction was performed for 3 hours or more using hot water of 80 ° C or higher. The mixture was separated by solid-liquid, and the liquid components were collected and used as crude extracts of each natural powder. The crude extract was left to separate the water layer and the oil layer, and after collecting only the oil layer, concentrated by applying steam treatment or the like to prepare each concentrated natural product extract.

2) Determination of active ingredient composition for the control of chicken mite

With the composition below, chicken mite remedies were tested according to the method described above. Including the active ingredient suggested in the experimental example below, the remaining amount excluding the active ingredient was applied sterile water, and the results are shown in Tables 1 to 3 below.

Experimental Example 1: 100% by weight of grapefruit seed extract

Experimental Example 2: 100% by weight of cloves extract

Experimental Example 3: 80% by weight of clove extract and 20% by weight of grapefruit seed extract

Experimental Example 4: 80% by weight of cloves extract

Experimental Example 5: Clove extract 50% by weight

Experimental Example 6: 10% by weight of cloves extract

Experimental Example 7: 10% by weight of clove extract and 20% by weight of castor oil

Experimental Example 8: 10% by weight of clove extract and 20% by weight of cinnamon oil

Experimental Example 9: 10% by weight of clove extract, 20% by weight of castor oil, and 20% by weight of cinnamon oil

An untreated group was applied as a control group, and tannic acid and commercially available pesticides, abamectin (positive control 1) and emamectin benzoate (positive control 2), were applied at concentrations indicated in the table.

Treatment tool and treatment concentration (2000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Control 0 0 Control 0 Control 0 Average 0 Treatment tool and treatment concentration (2000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Positive Control 1 One 10 Positive Control 1 One Positive Control 1 One Treatment tool and treatment concentration (2000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Positive Control 2 One 10 Positive Control 2 One Positive Control 2 One Average One Treatment tool and treatment concentration (4000ppm) Number of insecticides per 20 chicken mites Insecticidal rate (%) Experimental Example 1 5 23.35 Experimental Example 1 6 Experimental Example 1 3 Average 4.67 Treatment tool and treatment concentration (2000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Experimental Example 2 10 97 Experimental Example 2 9 Experimental Example 2 10 Average 9.67

Treatment tool and treatment concentration (2000ppm) Number of insecticides per 20 chicken mites Insecticidal rate (%) Experimental Example 3 20 100 Experimental Example 3 20 Experimental Example 3 20 Average 20 Treatment tool and treatment concentration (2000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Experimental Example 4 10 100 Experimental Example 4 10 Experimental Example 4 10 Average 10 Treatment tool and treatment concentration (1000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Experimental Example 4 10 100 Experimental Example 4 10 Experimental Example 4 10 Average 10 Treatment tool and treatment concentration (2000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Experimental Example 5 10 100 Experimental Example 5 10 Experimental Example 5 10 Average 10 Treatment tool and treatment concentration (1000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) Experimental Example 5 10 100 Experimental Example 5 10 Experimental Example 5 10 Average 10

Treatment tool and treatment concentration (1000ppm) Wagumo insecticidal rate (%) Experimental Example 6 68% Experimental Example 7 78% Experimental Example 8 87% Experimental Example 9 99%

With reference to the experimental results of Tables 1 to 3 above, active ingredient 1 (clove extract), active ingredient 2 (clove extract + castor oil), active ingredient 3 (clove extract + castor oil + cinnamon oil) It was selected as an active ingredient.

Chicken mite  Formulation and indoor test of remedy composition

1) Determination of formulation of chicken mite relief composition

Including the above active ingredients, an experiment was conducted to determine a formulation for use as a composition for controlling chicken mites. The active ingredients are extracts derived from natural products, and were applied while changing the ingredients and contents of the surfactant to which a formulation capable of improving the stability of the active material was applied. The above experiment was independently performed at least three times using a composition sample, and the results were averaged and shown in Table 4 below.

Liquid
Formulation
NO. Composition content (% by weight) * 1000ppm
Upon processing
Wagumo
Insecticidal rate (%) available
ingredient Interface
Activator 1 Interface
Activator 2 Interface
Activator 3 Interface
Activator 4 Formulation 1 50 20 - - - 99% Formulation 2 50 - 20 - - 55% Formulation 3 50 - - 20 - 60% Formulation 4 50 - - - 20 75% Formulation 5 50 10 10 10 15 80% Formulation 6 50 20 - - 20 99% Formulation 7 80 10 10 - - 100% No treatment - - - - - 0

Surfactant 1: glycols (polyethylene glycol or propylene glycol)

Surfactant 2: polyoxyethylene dodecyl mono ether

Surfactant 3: (1,1,3,3-tetramethylbutyl) phenyl ether [(1,1,3,3-tetramethylbutyl) phenyl ether]

Surfactant 4: sodium bis (2-ethylhexyl) sulfosuccinate

* Sterilized water is applied to the residual amount in weight percent.

With reference to the above experimental results, the surfactant applied to the formulation of the present invention was subjected to the following experiments with Formulation 1, Formulation 6, and Formulation 7.

2) Insecticidal indoor test using composition for remedy for chicken mites

The sample to which the formulation 7 was applied to the active ingredient 1 was named W0, the composition to which the formulation 6 was applied to the active ingredient 2, and the sample to which the formulation 6 or 7 was applied to the active ingredient 3 was designated as W2. Indoor test was conducted, and the results are shown in Tables 5 to 8 below. In addition, sample pictures of chicken mites (wagumo) taken in the experiment process of Table 6 are shown in FIG. 1. As a positive control, an externally free product containing bifenthrin was applied as an active ingredient.

Treatment tool and treatment concentration (1000ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) W0 10 100 W0 10 W0 10 Average 10 Treatment tool and treatment concentration (500ppm) Number of insecticides per 10 chicken mites Insecticidal rate (%) W0 10 100 W0 10 W0 10 Average 10

Treatment (diluted drainage) Insecticide Insecticidal rate (%) Remark W1 (1,000 times) 10 100 - W1 (1,000 times) 10 100 W1 (1,000 times) 10 100 Average 10 100 W1 (2,000 times) 9 90 - W1 (2,000 times) 10 100 W1 (2,000 times) 10 100 Average 9.67 96.67 Wagu free 1,000 times 8 80 Bifenthrin
10% Wagu free 1,000 times 8 80 Wagu free 1,000 times 8 80 Average 8 80 2,000 times of wagupuri 6 60 Bifenthrin
10% 2,000 times of waguri 6 60 2,000 times of wagupuri 7 70 Average 6.33 63.33 No treatment 0 0 - No treatment 0 0 No treatment 0 0 Average 0 0

Treatment (diluted drainage) Insecticide Insecticidal rate (%) Remark W2 (1,000 times) 10 100 - W2 (1,000 times) 10 100 W2 (1,000 times) 9 90 Average 9.67 96.67 W2 (2,000 times) 10 100 - W2 (2,000 times) 10 100 W2 (2,000 times) 9 90 Average 9.67 96.67 Wagu free 1,000 times 8 80 Bifenthrin
10% Wagu free 1,000 times 8 80 Wagu free 1,000 times 8 80 Average 8 80 2,000 times of wagupuri 6 60 Bifenthrin
10% 2,000 times of wagupuri 6 60 2,000 times of wagupuri 7 70 Average 6.33 63.33 No treatment 0 0 - No treatment 0 0 No treatment 0 0 Average 0 0

Referring to the above experimental results, it was confirmed that the formulation of the present invention can obtain an excellent chicken mite insecticidal effect.

Chicken mite  Relief composition Outdoor clinical insecticidal effect  exam

An outdoor clinical insecticidal effect test was conducted on the composition of the W0 formulation in Farm B, about 15,000 waters located in Cheonan.

Before and after drug spraying on chicken mites, the number of chicken mites was recorded by dividing into the dosing group and the control group, and drug spraying was first administered at a dilution concentration of 500 times after mite collection on Day 0, taking into account the life history of mites 1 After a week, the second dose was given at 500 times the concentration.

The geometric mean of the number of chicken mites, one week before (day -7) and just before application (day 0), was 376.0 and 500.6 in the drug-administered groups, and 397.0 and 420.6 in the control group, respectively, showing an increasing trend. There was no significant difference between the control group and the administration group (p = 0.954).

As a result of confirming the number of ticks on the 7th day after the first drug distribution, the average of 101.2 animals in the dosing group showed a decrease rate of 79.8%, and the control group showed an increase of 91.2% with an average of 804.0 animals. Thereafter, as a result of checking the number of chicken mites on the 14th, 21st, and 28th days, the dose group showed a slight increase on the 28th day with an average of 116.6, 103.5, and 135.0, respectively. The control group showed a steady increase of 895.5, 1109.9, and 1197.8 animals, respectively (see Fig. 2).

In addition, as a result of confirming the significance between the geometric mean of the number of ticks in each group, it was confirmed that the average of the dosing group was significantly lower than that of the control group over all periods after drug spraying (p <0.001).

The mean body weight, weight gain, and clinical symptoms of chickens measured during the experimental period were measured at 7, 14, 21, and 28 days a week after treatment with day 0 immediately before treatment. In the dosing group, the mean body weight increased from 1,831.1g to 1,980.9g during 28, and the control group increased from 1,766.5g to 1,968.7g, and it was confirmed that both groups continuously gained weight during the experimental period. In addition, when looking at the clinical symptoms, in the dosing group, a slight diarrhea was confirmed as an average of 0.1 on Day28 during the experimental period, and in the case of a control group, diarrhea was confirmed as a faint constipation at an average of 0.1 on Day21. It was judged that there would be no clinical significance to the extent that it could occur.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

Claims (13)

An active ingredient comprising a clove extract; And a veterinary composition for controlling chicken mite comprising a surfactant comprising a glycol,
The active ingredient further includes castor oil,
The active ingredient comprises the clove extract and the castor oil in a weight ratio of 1: 2 to 5.5,
The surfactant further includes any one selected from the group consisting of polyoxyethylene dodecyl monoether, sodium bis (2-ethylhexyl) sulfosuccinate, and combinations thereof,
The surfactant comprises the glycol and the sodium bis (2-ethylhexyl) sulfosuccinate in a weight ratio of 1: 0.3 to 1.1,
The surfactant contains the glycol and the polyoxyethylene dodecyl monoether in a weight ratio of 1: 0.2 to 0.9,
5 to 85% by weight of the active ingredient based on the entire veterinary composition for controlling the chicken mite, the surfactant contains 15 to 55% by weight and the remaining amount of sterilized water,
A veterinary composition for controlling chicken mites, comprising at least 10% by weight of the glycol. According to claim 1,
The glycol is any one selected from the group consisting of polyethylene glycol, propylene glycol, and combinations thereof, a veterinary composition for controlling chicken mites. delete delete delete delete delete delete According to claim 1,
The active ingredient further includes cinnamon oil,
Veterinary composition for remedy of chicken mites, comprising the clove extract and the cinnamon oil in a weight ratio of 1: 0.4 to 5. The method of claim 9,
The active ingredient is 1 to 4 parts by weight of the cinnamon oil based on 1 part by weight of the clove extract, a veterinary composition for the control of chicken mite. According to claim 1,
The chicken veterinary remedy veterinary composition is a liquid formulation that is applied in a manner sprayed around the cage and around the chicken veterinary composition. A manufacturing process for preparing the administration solution by diluting the veterinary composition for controlling chicken mite according to claim 1 in water; And a control process of spraying the administration solution around the house and the cage; including, chicken mite remedies method of chicken farm using chicken veterinary veterinary composition. The method of claim 12,
The dilution is that the veterinary composition for controlling chicken mites and the water are mixed in a weight ratio of 1: 150 to 1,000, and the control process is performed 2 to 4 times at intervals of 5 to 10 days, of a poultry farm. How to get rid of chicken mites.

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