KR102149424B1 - Antiseptic composition for Dermanyssus gallinae - Google Patents

Abstract

The present invention provides a control agent composition for chicken mites. According to an embodiment, the composition for controlling chicken mites may include Gosam extract and garlic oil as active ingredients. In the composition for controlling chicken mites, the Gosam extract is provided in a volume ratio of 85% to 95%, the garlic oil is provided in a volume ratio of 3% to 5%, and the excipient is provided in a volume ratio of 2% to 10%. Can be provided.

Description

Antiseptic composition for Dermanyssus gallinae}

The present invention relates to a control agent composition, and specifically, to a control agent composition for chicken mites.

Since long ago, chicken mites have been the most problematic in laying hens and breeders, and because they are suppressed by treating drugs at least 3 to 5 times per year on the farm, resistance is frequent due to reckless use of pesticides, and as time goes by, larger amounts of pesticides Because it is possible to obtain a control effect by using a pesticide with deadly poisonous toxicity, it is causing serious social problems that can affect the human body directly ingesting livestock products such as poisoning damage to workers, environmental toxicity, and egg residues. Accordingly, research on natural substances that can reduce and replace chemical pesticides, natural enemies, and biological pesticides such as microorganisms is being conducted, but most of them have low control efficacy or economical reasons, so they cannot obtain good reactions from farms. It has not been successful as a substitute for pesticides.

The chicken tick ( Dermanyssus gallinae ) is an ectoparasite that is widely distributed worldwide and causes significant economic losses to the laying hen industry. External parasites that damage chickens include mites, lice, fleas, mosquitoes, flies, and beetles. Among them, chicken mites are the most dominant species. They are often parasitic on chickens, wild birds and other rodents, and are good for hoarding. It is the most important bloodsucking parasite because it thrives and can lead to anemia, decreased productivity and even death in laying hens. It is also a vector of avian spirochetes, chickenpox virus, Newcastle virus, Chubaekri, poultry typhus and cholera. In cages with a very high density of them, they can cause disgust in people. During their life cycle, the stages of vampirism are usually Nymph and adult females, and in very rare cases males also vampiric, but larvae do not.

As such, they have relied primarily on chemical control to control the most threatening mites in the house. Organophosphorus, carbamate, pyrethroid insecticides, and acaricides have been generally used, and effective control was achieved at the beginning of use, but due to repeated use, resistant chicken mites appeared, making effective control more and more difficult. And, accordingly, overuse of pesticides and acaricides is a problem. In addition to the side effects of these chemicals, public concerns about the safety of these substances amplified, and as a result, diazinon, which was widely used for home use, was expelled from the market. Plant extracts and plant-derived substances have been studied a lot as alternative sources of insecticides, and although they are environmentally friendly substances with high biodegradability, they are not settled in the market because economic factors and effectiveness are lower than those of insecticides. However, since there is no control method that is as effective as chemical control, it is necessary to reduce the number of uses rather than completely exclude chemical control, and to lower the insecticide resistance of chicken mites and maintain low concentration-high efficiency by rotating and using insecticides by series. It is important.

As biological control, there is a control method using natural enemies and microorganisms. Since natural enemies and microbial control are only possible at low density, it is not effective in controlling chicken mites.

The present invention is to solve the problems of the prior art as described above, to provide a control agent composition for chicken mites using eco-friendly natural materials.

The present invention is to provide a control agent composition for chicken mites using eco-friendly natural substances having a better effect than conventional pesticides using chemical substances.

The object of the present invention is not limited thereto, and other objects that are not mentioned will be clearly understood by those skilled in the art from the following description.

The present invention provides a control agent composition for chicken mites.

According to an embodiment, the composition for controlling chicken mites may include Gosam extract and garlic oil as active ingredients.

The volume ratio of the garlic oil and the Gosam extract may be mixed in the range of 1:17 to 1:32.

The gosam extract is provided in a volume ratio of 85% to 95%, the garlic oil is provided in a volume ratio of 3% to 5%, and an excipient may be provided in a volume ratio of 2% to 10%.

The composition for controlling chicken mites may be diluted with water in a ratio of 1:100 and provided.

According to another embodiment of the present invention, the composition for controlling chicken mites may be diluted with water in a ratio of 1:100 to 1:400 and provided.

According to an embodiment of the present invention, the composition for controlling chicken mites has an excellent control effect on chicken mites.

In addition, according to an embodiment of the present invention, the composition for controlling chicken mites may have a better insecticidal effect than pesticides using conventional chemical substances.

The effects of the present invention are not limited to the above-described effects, and effects that are not mentioned will be clearly understood by those of ordinary skill in the art from the present specification and the accompanying drawings.

The present invention relates to a composition for controlling chicken mites. The present invention can provide a control agent composition for chicken mites using eco-friendly natural materials. The composition for controlling chicken mites according to the present invention may be composed of Gosam extract and garlic oil as active ingredients. The control agent composition for chicken mites according to the present invention may have a higher control effect on chicken mites by mixing and applying Gosam extract and garlic oil in a certain ratio. The control agent composition for chicken mites may be applied after being diluted with water in a certain ratio, thereby increasing the control effect for chicken mites. Hereinafter, the present invention will be described in detail by examples, but the scope of the present invention is not limited by these examples.

Sophora flavescens Ait is a perennial plant belonging to the legume family. It is non-toxic, has a strong bitter taste, and is persistent, and is known to be effective in diuresis, dysentery, antipyretic, eczema, and antiarrhythmic in oriental medicine. As ingredients, several kinds of quinolizidine, alkaloid, flavonoid, saponin, etc. have been reported, and recently, the antibacterial and antioxidant effects of Gosam extract have been reported.

In the present invention, the gosam extract obtained from the gosam can be obtained using a hot water extraction method or an alcohol extraction method generally used in the art.

Garlic (Allium sativum L.) is a representative active substance, which is a pesticide of rice weevil, separated 1-3, diphenyl thioure and allitin. This inhibits the enzyme activity of insects and appears to have the effect of protecting stored grains from pests when stored with grains. Garlic oil can be prepared using the garlic as a raw material.

Excipient is a material used to shape the product when manufacturing drugs. The excipient according to the present invention may be any one of lactose, microcrystalline cellulose, and starch, but is not limited thereto.

Hereinafter, the results of the experiment for confirming the pesticide rate using Gosam extract and garlic oil according to the present invention will be described using a table.

The insecticidal rate test of chicken mites according to the present invention is as follows. A plastic dish of 89*89*25 mm size was used. A 55mm filter paper was put in the plastic weighing dish, 20 chicken mites were put, and then the drug was sprayed using a spray empty bottle. At this time, the number of sprays may be about 12 times. The drug is a control agent composition for chicken mites according to the present invention. The spray dose was about 0.8 ml. The environmental conditions during the experiment may be a temperature of 25±2°C and a humidity of 50±5%. After applying the drug using a spray under the environmental conditions as described above, the insecticidal rate was checked after 24 hours.

Table 1 is a table showing the results of the confirmation experiment on the pesticide rate after 24 hours after spraying the composition and the control group (Elector PSP, DI Water) including the Gosam extract on chicken mites. Experiments were conducted at various dilution ratios of 1:100, 1:200, 1:400 and 1:800, respectively. As a control agent composition for chicken mites, a composition mixed with 90% gosam extract, 3% garlic oil, and 7% excipient was used, and among the control groups, Elector PSP is a control agent containing chemical substances currently in use on the market. Was used, and DW is distilled water among the controls.

1:100 1:200 1:400 1:800 Gosam extract 94.1 ± 6.7 85.4 ± 4.9 66.1 ± 5.2 48.6 ± 5.7 Control (Elector PSP) 90.1 ± 2.5 83.7 ± 1.8 61.4 ± 4.7 41.5 ± 5.2 Control (D.W) 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0

[Insecticide rate confirmation experiment after 24 hours of spraying (%: mortality rate)]

Referring to Table 1, it can be seen that the experimental results of the control agent composition for chicken mites containing Gosam extract show a higher insecticidal effect compared to the distilled water control and the commercially available chemical control. It can be seen that even if the dilution concentration is divided from 1:100 to 1:800, the same trend is shown, and in all the experimental results, it can be seen that the effect is higher than that of the commercial chemical control.

In addition, it can be seen that the higher the dilution concentration, the greater the difference between the insecticide rate and the control group.

Tables 2 to 3 below are a table showing the results of an hourly insecticidal rate of the control agent composition for chicken mites diluted 1:100 and the hourly insecticidal rate of the control agent composition for chicken mites diluted 1:200 Corresponds to.

1 h 2 h 4 h 8 h 24 h Gosam extract 71±3.8 88±4.7 90±5.2 90±5.8 94±4.5 Control (Elector PSP) 59±4.1 75±5.2 75±4.3 86±3.9 90±5.8 Control (D.W) 0±0.0 0±0.0 0±0.0 0±0.0 0±0.

[100 times diluted solution hourly insecticide rate confirmation experiment (%: mortality rate)]

1 h 2 h 4 h 8 h 24 h Gosam extract 53±4.2 58±3.5 75±4.6 75±4.9 88±5.3 Control (Elector PSP) 38±2.8 47±3.9 61±5.1 67±5.6 83±4.5 Control (D.W) 0±0.0 0±0.0 0±0.0 0±0.0 0±0.

[200 times diluted solution hourly insecticide rate confirmation experiment (%: mortality rate)]

According to Tables 2 and 3, it can be seen that the diluted solution diluted 1:100 exhibits a higher insecticidal rate than the diluted solution diluted 1:200. In other words, the higher the concentration of the diluent, the higher the insecticide rate.

In addition, both Tables 2 and 3 showed increasingly higher insecticidal rates as time passed, so it can be seen that the highest insecticidal effect was exhibited when 24 hours passed after applying the control agent. It should be noted that, when 4 hours and 8 hours passed, the effect of the chicken mite control agent composition containing the Gosam extract according to the present invention was almost the same, but after 24 hours, the effect was much higher than that. There is a need.

In the above Tables 1 to 3, the chicken mite control agent composition according to the present invention has a better insecticidal effect than the conventional chemical control agent, and the experiment on the insecticidal effect according to the concentration of the diluent, and the change of time The experiment on the insecticidal effect was shown.

Tables 4 to 5 below are tables showing the results of experiments by changing the composition ratio inside the chicken mite control agent composition according to the present invention. Hereinafter, the effect exhibited by changing the said composition ratio is demonstrated.

1:100 1:200 1:400 Sophora extract 90% + excipient 10% 79.2 ± 3.8 67.4 ± 4.1 44.2 ± 4.2 Gosam extract 90% + Garlic oil 5% ¹⁾ 92.5 ± 3.4 80.9 ± 3.2 62.1 ± 2.9 Gosam extract 80% + Garlic oil 5% ²⁾ 86.7 ± 4.8 71.5 ± 3.8 50.4 ± 5.8 Ginseng extract 70% + garlic oil 5% ³⁾ 75.8 ± 5.1 64.7 ± 3.4 45.3 ± 4.6 Control (D.W) 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0

[Insecticide rate confirmation experiment by percentage of Gosam extract (%: mortality rate)]

1:100 1:200 1:400 Gosam extract 90% + Garlic oil 7% ¹⁾ 89.3 ± 4.6 78.7 ± 3.8 55.8 ± 5.4 Sophora extract 90% + Garlic oil 5% ²⁾ 91.7 ± 2.9 81.5 ± 3.3 60.3 ± 4.1 Gosam extract 90% + Garlic oil 3% ³⁾ 94.8 ± 3.7 86.1 ± 4.2 65.8 ± 4.5 Gosam extract 90% + Garlic oil 1% ⁴⁾ 88.6 ± 4.8 74.1 ± 2.9 52.9 ± 4.4 Control (D.W) 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0

[Insecticide Rate Verification Test by Garlic Oil Ratio (%: Mortality Rate)]

Table 4 is a table showing the results of an experiment to confirm the pesticide rate of a composition in which the ratio of the gosam extract was adjusted while the ratio of the garlic oil was fixed. Table 5 is a table showing the results of an experiment to confirm the insecticide rate of a composition in which the ratio of garlic oil was adjusted while the ratio of the Gosam extract was fixed.

According to the results shown in row 1 of Table 4, it can be seen that the insecticidal rate is not high even in the result of dilution at a ratio of 1:100 when only the gosam extract is included and the garlic oil is not included. It can be seen that this is a lower value even when compared with the control group in Tables 1 to 3. As a result, it can be seen that the insecticidal effect exhibited by the composition of Gosam extract and garlic oil is great.

According to the results shown in rows 2 to 4 of Table 4, the ratio of the garlic oil is fixed at 5%, and the results of the experiment are shown by adjusting only the proportion of Gosam extract to 90%, 80%, and 70%. According to Table 4, it can be seen that the case where the ratio of the garlic oil is 5% and the ratio of the Gosam extract is 90% has the highest insecticidal rate as 92.5% (error rate 3.4%). It can be seen that the lower the ratio of Gosam extract, the significantly lower the insecticide rate.

The result obtained in Table 4 is that high insecticidal effect can be obtained only when the Gosam extract and garlic oil are formulated in a certain ratio.

In Table 5, the ratio of Gosam extract was fixed at 90%, and the experiment was performed with a composition in which only the ratio of garlic oil was changed to 1% to 7%.

According to Table 5, there was a change in the insecticidal rate less than that of Table 4, but it can be seen that the insecticidal rate of 90% or more was exhibited when the garlic oil contained 3% to 5% in a situation where the Gosam extract was 90%. have.

That is, since the insecticidal rate of the control group diluted 1:100 is 90.1%, in the present invention, characteristics of the composition containing Gosam extract and garlic oil having an insecticidal rate of 90% or more were identified.

Among the experimental data in Tables 1 to 5, the ratio of the composition having the highest insecticide rate in 1:100 dilution corresponds to a composition in which 90% of Gosam extract and 3% of garlic oil are mixed.

In addition, garlic oil exhibited the highest insecticidal rate when it was 3% to 5%, and the Gosam extract showed the highest insecticide rate at 90%, so the Gosam extract according to the present invention was 85% by using the 90% as the median value. It is included in a ratio of% to 95%, and garlic oil may be included in a ratio of 3% to 5%.

According to the ratio of the composition, the ratio of the garlic oil and the gosam extract may be provided with a minimum ratio of 1:17 when the garlic oil is 5% and the gosam extract is 85%, and the garlic oil is 3%, and the gosam extract is At 95%, a maximum ratio of about 1:32 can be provided.

Among the experimental data, 3% of garlic oil and 90% of Sophora extract, which have the highest insecticide rate, have a ratio of 1:30, and may be included in the above range.

When the Ginseng extract and garlic oil are included as described above, the excipient may be provided in the amount of the remainder under the condition that the total ratio of the composition is 100%.

Claims (5)

Contains gosam extract and garlic oil as active ingredients,
The control agent composition for chicken mites, characterized in that the volume ratio of the garlic oil and the gosam extract is mixed in the range of 1:17 to 1:32.
delete The method of claim 1,
The Gosam extract is provided in a volume ratio of 85% to 95%, the garlic oil is provided in a volume ratio of 3% to 5%, and the excipient is provided in a volume ratio of 2% to 10%.
The method of claim 3,
The control agent composition for the chicken mite,
A composition for controlling chicken mites, characterized in that it is provided diluted with water in a ratio of 1:100.
The method of claim 3,
The control agent composition for the chicken mite,
Control agent composition for chicken mites, characterized in that provided diluted in a ratio of 1:100 to 1:400 with water.