KR102492381B1 - A composition for controlling poultry red mites - Google Patents

Abstract

The present invention relates to a composition capable of controlling mites, which are ectoparasites of poultry, and a method for controlling poultry mites using the same.

Description

Composition for controlling chicken mites {A composition for controlling poultry red mites}

The present invention relates to a composition capable of controlling mites, which are ectoparasites of poultry, and a method for controlling poultry mites using the same.

Poultry red mite ( Dermanyssus) gallinae ) is an ectoparasite of poultry, mainly chickens, called wagumo. They are parasitic on chicken skin and cause diseases such as poultry cholera through blood sucking, anemia, decrease in body weight gain, and decrease in egg laying rate of laying hens. According to data from the National Institute of Livestock Science of the Rural Development Administration in 2016, about 94% of domestic egg-laying farms are contaminated with chicken mites, and it is reported that more than 105 billion won of damage occurs every year. Chicken ticks are nocturnal and hide in dark places with high humidity, such as exhaust vents and inside cages during the day, and then suck blood during the night. In particular, in summer, when the temperature and humidity in the cage are high, the breeding of chicken ticks is very fast, and the economic damage to laying hens farms also increases. The representative control method is the administration of insecticides, which are currently sprayed on the skin such as pyridaben, nalide, bifenthrin, spinosad, cypermethrin, chlorpyrifos, and propoxer. There is a disadvantage that it is harmful to chickens, and when spraying chickens for control, toxicity may be induced not only to chickens in contact with the pesticide but also to the sprayer. In addition, the drugs induce resistance, and as the frequency of use increases, the control effect decreases. The representative pesticide damage is the fipronil incident that became a global issue. In the case of fluralaner, which is diluted in drinking water and administered orally, tolerance has not been reported, but it is sold at a high price and is difficult to use in small-scale farms.

As a method for controlling chicken mites, Korean Patent No. 10-2098728 discloses a composition for killing chicken mites containing caprylic acid, and Korean Patent No. 10-2096721 discloses a low-toxic, eco-friendly chicken mite control method using oxalic acid. Although the composition is disclosed, development of an effective chicken mite control composition that is not resistant to chicken mite control, is economical, and does not cause biotoxicity because it contains non-agrochemical components is required.

The problem to be solved by the present invention is to provide a composition for controlling chicken ticks.

The problem to be solved by the present invention is to provide a use of a composition for controlling chicken ticks.

In order to solve the above problems, the present invention provides a composition for controlling chicken mites (Dermanyssus gallinae) containing ivermectin and allicin.

Ivermectin is a non-agrochemical drug with low harmfulness and is used to kill harmful parasites such as lice and scabies. There are many glutamate-gated chloride channels in the body of mites or mites, and ivermectin can overexcite these channels to kill mites or mites. Ivermectin has not been approved as a drug for use in chickens, but the residual tolerance standard (0.01 mg/kg) has been set for domestic livestock products (poultry meat, eggs, etc.), and it is a component that can be applied to poultry.

The structure of ivermectin is shown in Formula 1 below.

[Formula 1]

According to the present invention, the composition may include ivermectin at a concentration of 0.4 to 2 mg/mL. If the content of the composition is less than the above range, the effect of killing chicken mites is insignificant, and if the content exceeds the above concentration, the increase in the killing effect of chicken mites is insignificant, so it is preferably within the above range. According to the present invention, the ivermectin may be preferably included at a concentration of 0.45 to 1 mg/mL, particularly preferably at a concentration of 0.5 to 0.6 mg/mL. When ivermectin is included at a concentration of 0.5 to 0.6 mg/mL, it is preferable because there is almost no toxicity, a remarkable effect is shown at a low drug concentration, and the degree of stress added to the subject is low.

Allicin is known to have the property of decomposing bacterial proteins. When allicin is broken down in metabolism, a component called sulfenic acid is produced. The chemical structure of allicin is shown in Formula 2 below.

[Formula 2]

According to the present invention, the composition may include allicin at a concentration of 0.4 to 2 mg/mL. If the content of the composition is less than the above range, the effect of killing chicken mites is insignificant, and if the content exceeds the above concentration, the increase in the killing effect of chicken mites is insignificant, so it is preferably within the above range. According to the present invention, the allicin may be preferably included in a concentration of 0.45 to 1 mg/mL, particularly preferably in a concentration of 0.5 to 0.6 mg/mL. When allicin is included at a concentration of 0.5 to 0.6 mg/mL, toxicity is very low, a significant effect is shown at a low drug concentration, and the degree of stress added to the subject is low, so it is preferable.

According to the present invention, the composition may include ivermectin at a concentration of 0.4 to 1 mg/mL and allicin at a concentration of 0.4 to 1 mg/mL.

According to the present invention, the composition may be sprayed externally or orally administered to a subject.

If spraying is desired, it may be formulated with various formulations known in the art, including the above components. Specifically, it may be formulated into solutions, emulsions, wettable powders, aqueous suspensions, oily suspensions, powders, powders, pastes, soluble powders, soluble granules, spray granules, aerosols, sprays, and the like. In these formulations, it may be included in the range of 0.01 to 20% by weight, preferably in the range of 0.1 to 10% by weight, depending on the formulation type or the formulation method accordingly. When formulated, it may be formulated by including carriers, emulsifiers, and other auxiliary agents for formulation, depending on the formulation type or the formulation method accordingly.

As the carrier, any carrier known in the art may be used. Suitable solid carriers include, among others, ammonium salts and ground natural minerals such as kaolin, clay, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth, finely divided silica, alumina, silicates and the like. Suitable solid carriers for granules are, for example, crushed and graded natural rocks such as calcite, marble, pumice, meerschaum and dolomite, granules of organic material such as paper, sawdust, coconut shells, corncobs, tobacco stalks. Suitable liquid carriers include, for example, water, alcohols such as methanol and ethanol, ketones such as acetone and methyl ethyl ketone, benzene and toluene, aromatic hydrocarbons such as xylene and ethylbenzene, hexane and cyclohexane. And aliphatic hydrocarbons such as kerosene or light oil, esters such as ethyl acetate or butyl acetate, nitriles such as acetonitrile or isobutyronitrile, ethers such as diisopropyl ether or dioxane, N, N -Acid amides such as dimethylformamide and N,N-dimethylacetamide, halogenated hydrocarbons such as dichloromethane and trichloroethane, dimethyl sulfoxide, and vegetable oils such as soybean oil and cottonseed oil. Suitable gas carriers include, for example, butane gas, LPG (liquefied petroleum gas), dimethyl ether, carbon dioxide gas and the like.

Emulsifiers can be either ionic or nonionic emulsifiers. As suitable emulsifiers, sulfuric acid alkyl esters, alkylsulfonic acid salts, alkylarylsulfonic acid salts, alkylaryl ethers and their polyoxyethylene adducts), polyethylene glycol ethers, polyhydric alcohol esters, and sugar alcohol derivatives.

Examples of auxiliary agents for preparation include fixing agents and stabilizers.

Examples of the fixing agent include polysaccharides such as casein, gelatin, and starch, cellulose derivatives such as starch, gum arabic, and carboxymethyl cellulose, alginic acid, etc.), lignin derivatives, and bentonite. ), synthetic water-soluble polymers (polyvinyl alcohol, polyvinylpyrrolidone, polyacrylic acids, etc.) and the like, and examples of stabilizers include acidic isopropyl phosphate (PAP), 2,6-di-tert -Butyl-4-methylphenol (BHT), a mixture of 2-tert-butyl-4-methoxyphenol and 3-tert-butyl-4-methoxyphenol (BHA), vegetable oil, mineral oil, fatty acids and their esters, etc. can be heard

Formulation types and formulation methods are well known in the art, and more specific references include Crop Life International and in Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides, FAO Plant Production and Protection Papers - 173, prepared by FAO/WHO Joint Meeting on Pesticide Specifications, 2004, ISBN: 9251048576].

According to the present invention, the composition can directly or selectively kill chicken mites.

In the present invention, the subject is a human or non-human. For example, it refers to primates, mammals, vertebrates, and poultry. In certain embodiments, the subject is poultry.

In addition, the present invention provides a feed additive composition comprising the composition for controlling chicken ticks containing ivermectin and allicin, and the type of feed is not limited.

In the present invention, the feed may be fish, bird, or mammal feed, and preferably, the wild habits defined in Article 2, Subparagraph 1 of the Livestock Act and each subparagraph of the Enforcement Rule of the same Act are purified and suitable for breeding. It can be feed for livestock or aquatic organisms that can contribute to farm household income increase. The livestock may be cattle, horses, mules, donkeys, goats, goats, sheep, deer, pigs, rabbits, poultry, etc., and poultry may be chickens, turkeys, ducks, ostriches, geese, quails, etc., preferably chickens, It is not limited thereto as long as it is suitable for breeding and obtaining livestock products. The above “livestock products” are meat, milk, eggs, honey and their processed products, hides (including raw fur), raw wool, and other livestock products produced from livestock, which are defined in Article 2, Subparagraph 3 of the Livestock Act, as determined by the Ordinance of the Ministry of Agriculture and Forestry. means that In addition, it may be a dog, cat, etc., including companion animals, but is not limited thereto.

The term "feed" in the present invention means any natural or artificial diet, meal, etc. or component of said meal, intended for or suitable for eating, ingestion and digestion by an animal. In one aspect, the feed composition containing the bell pepper extract of the present invention may include concentrated feed, roughage and / or special feed.

Concentrated feed includes seed fruits including grains such as wheat, oats, and corn, bran including rice bran, wheat bran, and barley bran as by-products obtained after refining grains, soybeans, fluids, sesame seeds, linseed, and coco palm oil. Fish meal, which is a by-product obtained by extracting starch from sweet potatoes and potatoes, and residual starch, which is the main component of starch residues, fish meal, fish residue, and fish soluble, meat meal, which are concentrated fresh liquids obtained from fish. , animal feed such as blood meal, feather meal, skim milk powder, dried whey obtained by drying whey, which is the balance when cheese is produced from milk and casein is produced from skim milk, yeast, chlorella, and seaweed.

Forage, raw grass feed such as wild grass, grass, and green cutting, turnip for feed, beet for feed, root vegetables such as Lutherbearer, a type of turnip, raw grass, green cut crops, grain, etc. are put into silos. There are silage (silage), which is a stored feed filled and fermented with lactic acid, grass, hay cut and dried, straw from breeding crops, and leaves of leguminous plants. Special feeds include mineral feeds such as oyster shells and rock salt, urea feeds such as urea or its derivative, diureide isobutane, and supplements for ingredients that tend to be insufficient when only natural feed ingredients are mixed, or formulated feeds to improve the storage life of feeds. There are feed additives, which are substances added in small amounts.

In addition, the composition according to the present invention can be used for various purposes and uses requiring the death of chicken mites. Specifically, it can be used as a skin cleaner, hair cleaner, mouthwash, bathroom cleaner, kitchen cleaner, food additive, feed additive, or disinfectant.

The present invention provides a detergent composition for cleaning a barn, comprising the composition containing the ivermectin and allicin.

In addition, the present invention provides a method for controlling chicken mites by administering or spraying a composition containing ivermectin and allicin to animals infected with chicken mites. and contacting a composition containing ivermectin and allicin of the present invention with a chicken mite. In the method of the present invention, contact can be made by spraying, spraying, or applying the composition of the present invention directly to chickens infected with chicken mites. In this case, only the insecticide composition of the present invention can be used, but it can also be used in combination with other insecticides, nematicides, acaricides, animal feeds, etc. According to the present invention, animals infected with chicken mites may be domestic animals, preferably poultry, more preferably chickens or chicks.

The composition containing ivermectin and allicin according to the present invention can control chicken mites with significantly improved efficiency compared to single treatment, has low cytotoxicity, and is economical, so it can be usefully applied to farms in need of chicken mite control. do.

1 is a visual confirmation of the death of chicken mites left in a Petri dish coated with ivermectin and allicin.
Figure 2 shows the killing effect of chicken mites according to the time left in the petri dish coated with ivermectin, allicin and ivermectin + allicin.

Advantages and features of the present invention, and how to achieve them, will become clear with reference to the detailed description of the following embodiments. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various different forms, and only the present embodiments will complete the disclosure of the present invention and allow common knowledge in the art to which the present invention belongs. It is provided to fully inform the holder of the scope of the invention, and the present invention is only defined by the scope of the claims.

Example

manufacturing example One.

A composition for controlling chicken mites was prepared by dissolving ivermectin and allicin in a 10% aqueous ethanol solution to contain a concentration of 0.5 mg/mL, respectively. As a control, a composition containing distilled water, 10% aqueous ethanol solution, ivermectin at 0.5 mg/mL, and a composition containing 0.5 and 1 mg/mL of allicin were prepared.

Test Example 2. Chicken mite control effect evaluation according to combined treatment

The chicken mite control effect of ivermectin and allicin alone and in combination was tested. After spraying 1 ml of control, ivermectin (0.5 mg/mL), allicin (0.5 and 1 mg/mL) and ivermectin + allicin (0.5 mg/mL each) on a Petri dish, it was dried for a certain period of time. 20 chicken ticks were placed in each petri dish and the number of chicken ticks surviving over time (2h, 24h, 48h, 7 days) was confirmed to confirm the mortality rate and the corrected insecticidal rate. (number of initiators = 20; IVM: ivermectin, ACN: allicin)

Extinction Rate: (Number of death mites/Number of initiation ticks)*100

Corrected insecticidal rate: (experimental group mortality rate - control group mortality rate) / (100 - control group mortality rate) * 100

Figure 1 shows the visual confirmation of death and survival of chicken ticks left in a Petri dish coated with ivermectin and allicin.

As shown in Figure 2, the treatment of ivermectin and allicin alone showed a low chicken mite control effect, but the combined treatment of ivermectin and allicin significantly improved the control effect, killing most chicken mites within 24 hours. .

In the group treated with ivermectin 0.5 mg/mL alone, an average of 7.67 chicken ticks were killed after 24 hours, and an average of 17.67 chicken ticks were killed only after 48 hours. Treatment with allicin alone had little control effect on chicken ticks, and in the group treated with 0.5 mg/mL of allicin as single ticks, only 2.33 chicken ticks were killed after 24 hours, and high concentration (1 mg/mL) killed 48 chicken ticks. Even with time treatment, only 4.67 animals could be killed. On the other hand, the combined treatment of ivermectin 0.5 mg/mL and allicin 0.5 mg/mL killed an average of 18.33 chicken mites after 24 hours of treatment, and the chicken mite control effect was evaluated to be very excellent.

Accordingly, it was confirmed that the combined treatment of ivermectin and allicin can significantly improve chicken mite control.

Test Example 2. Effect of spraying the sample

Twenty chicken ticks were put in a petri dish and the control effect was confirmed by directly spraying the sample on the chicken ticks by the direct contact method (filter-paper contact method; J Vet Sci. 2020 Mar; 21(2):e37). In the experimental group sprayed with 1 ml of ivermectin + allicin (each 0.5 mg/mL), it was confirmed that 19.33 birds were killed after 12 hours, confirming that the chicken mite control effect was excellent even when the sample was directly sprayed.

Claims (10)

Chicken mite ( Dermanyssus gallinae ) control composition comprising allicin at a concentration of 0.4 to 2 mg / mL ivermectin (ivermectin) and 0.4 to 2 mg / mL concentration. delete delete According to claim 1,
The ivermectin is included at a concentration of 0.45 to 1 mg/mL,
The allicin is contained in a concentration of 0.45 to 1 mg / mL, the composition. According to claim 1,
The composition is for external spraying or oral administration, the composition. According to claim 1,
Wherein the composition kills chicken mites. Claims 1, 4 to 6, comprising the composition according to any one of claims, chicken mites ( Dermanyssus gallinae ) feed additive composition for control. Claims 1, 4 to 6, comprising a composition according to any one of claims, a cleaning composition for barn cleaning. A chicken mite control method comprising administering or spraying the composition according to any one of claims 1, 4 to 6 to animals. 10. The method of claim 9, wherein the animal is poultry or livestock.

Images