KR20180021417A - Compositions for controlling poultry red mite comprising extracts derived from natural materials and preparation method of the same - Google Patents

Abstract

The present invention relates to a veterinary medical composition for controlling Dermanyssus gallinae containing extracts from natural materials as active ingredients, and a production method of the composition. The veterinary medical composition contains one extract of natural materials selected from the group consisting of a grapefruit seed extract, a dried chrysanthemum extract, a tea tree extract, a clove tree extract, and combinations thereof, as an active ingredient, and shows an excellent effect on controlling Dermanyssus gallinae with a low concentration. The veterinary medical composition utilizes a substance originated from natural materials, thereby being eco-friendly, and having minor harmfulness to the human body.

Description

TECHNICAL FIELD The present invention relates to a composition for relieving chick mites (natural worms) using natural products and a method for preparing the same,

The present invention relates to a composition for relieving chicken mites (worms) using natural products and a method for producing the composition.

Among the species of ticks, the most frequent species that occur in poultry are chicken mites (fowl red mite, Dermanyssus gallinae , and the northern fowl mite, Ornithonyssus sylviarum .

These chick ticks are distributed not only in Korea but also in the whole world, and they have a habit of being parasitized and blood-sucking in chickens. In particular, Wagumo is parasitized by the host chicken, causing blood vomiting, itching and anemia, and can lead to death in severe cases. This tick has the problem that it is very difficult to control because it shows the characteristic that it feeds the eggs and breeds eggs in the crevices of the cages as well as moves to the host only when blood is needed.

Chicken red om is the most important external parasite that causes problems in spawning in countries that currently carry out poultry industry including Korea. This species is found in almost all birds, including poultry, leafy and wild birds, and is often a nuisance to those working in poultry because it causes skin diseases by sucking mammals, sometimes including humans. In addition, the parasite acts as a mediator for viruses that cause various diseases related to chickens such as poultry typhus and poultry cholera.

It is known that more than 35 kinds of substances are used to control chicken ticks. Some of them are effective, but they are inappropriate in terms of food safety and environment. In addition, repeated and long-term use of medicines has resulted in the development of resistant mites, and there has not yet been developed a clear alternative medicine to replace them.

In recent years, there has been a recent problem of massive disposal of poultry due to avian influenza (AI), intestinal hepatitis, infectious bronchitis, and productivity problems, resulting in a sudden drop in the supply of poultry and shortage of poultry supply. , There has been a growing demand for antiseptic drugs for efficient and healthy poultry breeding while ensuring the stability of food, because consumers are dissatisfied with the use of the previously unreasonable antibiotics.

In addition, there is a growing awareness of safe food, increasing concern about the possibility of residual pesticides in poultry or poultry of pesticides or poultry, and there is a growing demand in the market to refrain from the use of chemicals such as pesticides. Therefore, it is necessary to develop technology that can save chicken mites (wagomo) in an environmentally friendly and safe way.

Korean Patent No. 10-1505018, published on Mar. 23, 2015, a method for producing an insect pesticide and a pesticide remover Korean Patent Laid-Open Publication No. 10-2013-0142588, December 30, 2013, insoluble composition containing insoluble essential oils and / or plant additives

An object of the present invention is to provide a veterinary composition for relieving chicken mites containing a natural extract as an active ingredient and a method for producing the same.

In order to achieve the above object, a veterinary composition for relieving chicken mites comprising an extract of natural materials according to an embodiment of the present invention is characterized in that grapefruit seed extract, dried chrysanthemum extract, tea tree extract, clove extract , And a combination thereof.

The veterinary composition may further comprise any one solvent or excipient selected from the group consisting of ethanol, water, a surfactant, and combinations thereof.

The veterinary composition may contain at least 50% by weight of the clove extract as the natural extract.

The method for relieving chicken mites of poultry according to another embodiment of the present invention includes a step of spraying the above-described veterinary composition onto poultry and / or poultry houses.

The veterinary composition includes any one selected from the group consisting of grapefruit seed extract, dried chrysanthemum extract, chrysanthemum extract, clove extract, and combinations thereof as an effective ingredient.

The veterinary composition may further comprise any one solvent or excipient selected from the group consisting of ethanol, water, a surfactant, and combinations thereof.

The veterinary composition may contain at least 50% by weight of the clove extract as the natural extract.

According to another embodiment of the present invention, there is provided a method for preparing a veterinary composition for relieving chicken mites, which comprises pulverizing a natural product selected from the group consisting of grapefruit seeds, chrysanthemum, tea, clove, Preparing a powder; An extracting step of mixing the natural product powder with an extraction solvent to conduct an extraction process, and then removing a solid portion to obtain a crude extract; And a separation step of separating the crude extract into a water layer and an oil layer and separating the crude oil-derived oil layer from the natural oil-derived oil layer, thereby preparing a natural product extract comprising the natural oil-derived oil layer.

In the extraction step, the extraction solvent may be hot water.

The natural product powder and the hot water may be mixed at a weight ratio of 100: 20-100.

The natural product extract may include the clove-derived oil layer.

The veterinary composition for relieving chicken mites may contain not less than 50% by weight of the clove-derived oil layer based on the entire veterinary composition for chicken mite remedies.

In describing the present invention, the singular forms of the present invention are not limited to singular and plural, except for the case where it is obvious that the singular form is singular in the context of the present invention. do.

Or " and / or "are used interchangeably and the description of" A or B "or" A and / or B " Can be interpreted as including meaning.

Hereinafter, the present invention will be described in more detail.

The inventors of the present invention have found that chicken mites (such as moths, red mites, Dermanyssus , etc.) , which cause particularly serious damage to poultry farms, gallinae ), but also to solve the two problems of food safety such as poultry meat and eggs. In order to solve these two problems simultaneously, it is necessary to use effective natural remedy But it is possible to secure food stability, and it can be remedied by an environmentally friendly method. Thus, the present invention has been completed.

The veterinary composition for relieving chicken mites comprising an extract of natural materials according to one embodiment of the present invention is useful as an effective ingredient of grapefruit seed extract, dried chrysanthemum extract, tea tree extract, clove extract, and combinations thereof And < / RTI >

The grapefruit seed extract is Citrus Paradisi (Grapefruit Seed Extract), which is produced by a process for preparing natural extracts described below. Grapefruit is a fruit of grapefruit tree belonging to the evergreen tree of the perennial herbaceous perennial herbaceous perennials, and is distributed in the temperate and subtropical regions of the West Indies. Grapefruit seed extract contains Naringin, natural ascorbic acid, and tocopherol.

Chrysanthemum extract, Chrysanthemum ( Echinacea) purpurea , purple coneflower), which is produced by a process of producing a natural substance extract described below using leaves, stems, roots, flowers, seeds or outposts. Chrysanthemum is a pair of green leaves of North American origin. Leaves are alternate phyllotaxis, egg-shaped, egg-shaped and usually serrated. Flowers are bloomed with head-shaped flowers and lanceolate. And the lanceolate is spreading to 7.5 cm in length or hanging downward.

The tea tree extract is an extract of Camellia sinensis , and can be applied to a plant produced by a process of producing a natural material extract described below using leaves, stems, roots, flowers, seeds or outposts. A tea tree is an evergreen tree or shrub with a lateral carpenteria, and is distributed in tropical, subtropical, and temperate regions, and is a plant with a height of 2 to 15 m.

The clove extract is an extract of cloves ( Eugenia caryophyllata thumb.), And may be prepared by a process for preparing natural extracts described below using leaves, stems, roots, flowers, seeds or outposts. Clove is a tropical evergreen gourd, originating from the Moluccas, produced in Zanzibar Island in Tanzania, Sumatra in Indonesia, Brazil, Malaysia, Philippines, Vietnam and southern China. The buds are called cloves and the dried daffin flowers are said to be the mottles. The flower bud looks like a nail and has a fragrance called clove. It is used for edible or medicinal purposes.

In the present invention, the extract of these natural products is confirmed as an effective ingredient of a veterinary composition for relieving chicken mites by confirming the effectiveness of chicken mite remedy.

The natural product extracts may be used alone or in combination of two or more thereof, and they may be mixed with excipients and additives described below. However, the above natural product extract may be contained in an amount of 0.1 to 100% by weight, 30 to 100% by weight, or 50 to 99% by weight based on the total veterinary composition, Even when the active ingredient is applied in a minimum amount, a good controlling effect can be obtained.

The veterinary composition may include a grapefruit seed extract, and may be applied in the form of a mixture of grapefruit seed extract and clove extract, wherein the blending ratio of the clove extract: grapefruit seed extract is 10 to 90: 10 in a weight ratio of 50 to 90: 50 to 10, and in this case, a more effective chicken mite remedy effect can be obtained.

The veterinary composition may include a dried chrysanthemum extract, and the dried chrysanthemum extract may be a mixture of a chrysanthemum extract or an extract of cloves.

The veterinary composition may include a tea extract, and the tea extract may be applied alone, but may be applied in the form of a mixture with a dried chrysanthemum extract, a mixture with ethanol, or a mixture with an extract of cloves. The tea extracts are mixed to help maintain and improve the effectiveness of chicken tick remedies.

The veterinary composition may include clove extract, a mixture with the grapefruit seed extract, a mixture with the tea extract, and the like, and may further comprise a solvent such as sterilized water to constitute the composition have.

The clove extract may be any one selected from the group consisting of clove leaf extract, clove flower extract, clove bud extract, clove extract of cloves, and combinations thereof, and may be used alone or in combination with other natural product extracts It shows strong chicken mite remedy effect when used.

The clove extract may be contained in the veterinary composition in an amount of 50% by weight or more, specifically 70% by weight or more, more specifically 80% by weight or more. A sufficient amount of the veterinary composition containing 50% by weight or more of the extract of cloves, which is an effective ingredient, is applied to the chicken mite remedy, and sufficient chicken mite control effect can be obtained.

In addition, when the clove extract is applied as a mixture with other natural extracts, the amount of the clove extract may be 5 to 50 parts by weight based on 100% of the clove extract. It is possible to obtain an advantageous effect or a synergistic effect by the other natural product extract while keeping it intact. For example, grapefruit seed extract, tea tree extract, and the like can be applied to the other natural material extracts, and in this case, a synergistic effect of the chicken mite remedy effect can be obtained.

The veterinary composition may further comprise any one solvent or excipient selected from the group consisting of ethanol, water, a surfactant, and combinations thereof.

At this time, the surfactant may be selected from the group consisting of polyethylene glycol, polyoxyethylene undecyl monoether, and combinations thereof.

In addition, the veterinary composition may further comprise additives such as a veterinarily acceptable carrier or excipient. In addition, the veterinary composition may be administered alone or in admixture with carriers, excipients, etc., and such dosage forms may be formulations for single-dose or multiple-dose administration.

The veterinary composition may be a solid or liquid preparation. The solid preparation includes powders, granules, tablets, suppositories, and the like, but is not limited thereto. Solid form preparations may include, but are not limited to, carriers, flavoring agents, binders, preservatives, disintegrants, lubricants, fillers, excipients and the like. Examples of the liquid preparation include water, a solution such as a solution of propylene glycol, a suspension, an emulsion, and the like, but the present invention is not limited thereto. The liquid preparation may be prepared by adding a suitable coloring agent, flavoring agent, stabilizer, tackifier, . For example, powders can be prepared by simply mixing a compound or its salt, which is an active ingredient in the veterinary composition of the present invention, with a veterinarily acceptable carrier such as lactose, starch, microcrystalline cellulose and the like. The granules may contain the active ingredient of the present invention; A suitable veterinarily acceptable carrier; And a suitable binder, and then wet granulation using a solvent such as water, ethanol, isopropanol or the like, or dry granulation using a compressive force. In addition, the tablet can be prepared by mixing the granule with a suitable veterinarily acceptable lubricant such as magnesium stearate and then tableting using a tablet machine.

In addition, the veterinary composition can be formulated into a suitable unit dosage form, including conventional, non-toxic, veterinary or pharmaceutically acceptable carriers, additives, vehicles according to the route of administration or administration.

The veterinary composition of the present invention can be applied as much as the amount per poultry, and can be applied by spraying, spraying, or the like. Depending on the specific situation such as the degree of infection and relief of chicken mites, And the like can be adjusted and applied. Specifically, the concentration of the active ingredient in the poultry rearing area can be sprayed at a concentration of 5000 ppm or less at a time of spraying, sprayed at a concentration of 2000 ppm or less, sprayed at a concentration of 1000 ppm, As shown in Fig.

However, the application amount may vary depending on the content of the active ingredient contained in the veterinary composition, the state of the individual (body weight, etc.), the severity of the infection state of chick mite, and the like. If necessary, the total daily dose may be administered in divided doses several times a day.

In another embodiment of the present invention, the method for relieving chicken mites of poultry includes the step of spraying the above-described veterinary composition onto a poultry or poultry house.

By applying this method, environmentally friendly and safe chicken mite remediation can be carried out using natural materials, and the remedial effect is also excellent.

The detailed description of the veterinary composition is redundant with the description above, so the description thereof is omitted.

The method for preparing a veterinary composition for relieving chicken mites according to another embodiment of the present invention includes a step of producing a natural product extract containing a natural-material-derived oil layer.

The process for preparing a natural product extract comprising the natural-product-derived oil layer comprises preparing a natural product powder by pulverizing any one natural product selected from the group consisting of grapefruit seed, chrysanthemum, chrysanthemum, clove, and combinations thereof ; An extracting step of mixing the natural product powder with an extraction solvent to conduct an extraction process, and then removing a solid portion to obtain a crude extract; And a separating step of separating the crude extract into a water layer and an oil layer to separately obtain an oil layer derived from a natural product.

Specific details such as the content and content of the natural product are the same as those described above, so the description thereof will be omitted.

The drying can be carried out in a known manner to such an extent that the useful components from the collected natural product (plant) are not destroyed, and can be carried out, for example, by natural drying in the shade. In addition, it is preferable that the pulverization (pulverization) is pulverized to such an extent that useful components of the plant can be sufficiently extracted in the subsequent extraction process. The drying and crushing steps may be carried out in reverse order or repeatedly as required.

The extraction may be performed by, for example, hot water extraction, cold extraction, warming extraction, reflux cooling extraction, ultrasonic extraction, or the like, and preferably hot water extraction.

The extraction is carried out using an extraction solvent, which may be an alcohol of 1 to 5 carbon atoms, hexane, chloroform, alcohol, water and mixtures thereof, preferably water, methanol, ethanol, Lt; / RTI >

The extraction is carried out at a temperature of 20 to 100 DEG C for 30 minutes to 5 days using a solvent having a volume of 3 to 20 times volume, preferably 3 to 10 times volume based on the volume of the plant dry matter Can be applied, and the crude extract obtained by this method can be further subjected to a process of decompression, concentration and / or drying in the subsequent process.

Specifically, in the extraction step, hot water can be applied to the extraction solvent. In the case of applying the hot water, it is possible to proceed with a simple and efficient extraction step in comparison with the case of applying other extraction solvents such as methanol and ethanol, , It is possible to extract more useful components for chicken mite relief. The hot water may be water having a temperature of 80 ° C or higher during mixing.

The natural product powder and the hot water may be mixed at a weight ratio of 100: 20 to 100, and the extraction step may be performed. The natural product powder may be mixed and processed at a weight ratio of 100: 20 to 80, . In addition, the extraction time may be from 10 hours to 5 days, and may be from 1 day to 4 days. When mixing is carried out in such a weight ratio, the active ingredient for use in the chicken mite remedy can be extracted, and subsequent steps such as separation and concentration can be efficiently performed.

Specifically, the liquid crude extract may be separated from the dry matter of the plant by vacuum filtration or the like, and then subjected to a concentration or drying process. For example, the liquid crude extract may be concentrated in a vacuum rotary condenser at a temperature of 20 to 100 ° C, preferably 40 to 70 ° C, and the oil may be collected separately from the liquid extract, And may be applied after being pulverized. The concentrated or powdered extract may be used, if necessary, in water, alcohol, dimethyl sulfoxide (DMSO), or a mixed solvent thereof.

In addition, a method of extracting the above liquid extract (crude extract), its concentrate or its powder by fractionation with nucleic acid or ethyl acetate or fractionation of said fraction may be applied.

The method for preparing a veterinary composition may further comprise an excipient mixing step after the step of preparing the natural extract, wherein the excipient is selected from the group consisting of ethanol, water, a surfactant, Lt; / RTI > These excipients can be applied in an appropriate amount so as to facilitate the storage and spraying of the active ingredient, and to help the veterinary composition for relieving chicken mites function effectively.

The veterinary composition for the relief of chicken mites and the method for producing the same of the present invention have an excellent effect in relieving chicken mites at a low concentration while using natural extracts as an effective ingredient and have advantages of being environmentally friendly and minimally harmful to human body .

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In the following, the reference is made without any specific reference, and in the case where the ratio or the criterion based on% is unclear, it means the weight ratio or weight percentage based on the weight.

Manufacture of Natural Products Extract

Dried grape seeds, chrysanthemum powder, chrysanthemum powder, and clove powders were prepared by spraying, drying and pulverizing each of the seeds, seeds, chrysanthemum seeds, chrysanthemum leaves, and clove.

100 parts by weight of each of the natural product powders and 30 parts by weight of hot water were mixed to prepare a mixture. Extraction was carried out and extraction was carried out for 3 hours or more using hot water of 80 ° C or more.

The mixture was subjected to solid-liquid separation, and liquid components were collected and used as a crude extract of each natural product powder.

The crude extract was allowed to stand to separate the water layer and the oil layer. Only the oil layer was collected, and steam treatment or the like was applied to concentrate the extract to prepare concentrated natural product extracts.

Chicken tick  Preparation of remedial veterinary composition

The veterinary composition was prepared with the following composition using the extract prepared by the method described in the above, and the effect of chicken mite remedy was confirmed below.

1) Grapefruit seed extract

2) A mixture of 30% by weight of the chrysanthemum extract, 5% by weight of the tea extract and 65% by weight of sterilized water

3) Clove extract

4) A mixture of 30% by weight of a tea extract, 30% by weight of ethanol and 40% by weight of sterilized water

5) 80% by weight of clove extract and 20% by weight of grapefruit seed extract

6) A mixture of 70% by weight of clove extract, 5% by weight of a tea extract, and 25% by weight of sterilized water

7) A mixture of 80% by weight of an extract of clove and 20% by weight of sterilized water

8) A mixture of 50% by weight of clove extract, 5% by weight of a tea extract, and 45% by weight of sterilized water

9) A mixture of 50% by weight of the extract of clove and 50% by weight of sterilized water

10) A mixture of 80% by weight of an extract of clove, 10% by weight of polyethylene glycol and 10% by weight of polyoxyethylenedidecyl monoether

Chicken tick  Relief effect test

Filter paper NO.2 was placed on a nsect petri dish, and 3 ml of sterilized water was dispensed, followed by inoculation with chicken mite ( Dermanyssus gallinae ). Each of the test drugs was sprayed onto the fedrydish inoculated with chicken ticks at the concentrations listed in the table, and cultured at room temperature for 24 hours. After 24 hours, it was observed under a microscope to determine whether chicken ticks were killed. The results are shown in the respective tables below.

The above experiment was carried out at least three times independently using the same veterinary composition sample, and the control group was the untreated group. The positive control group was tannic acid and commercially available insecticides abamectin, emamectin benzoate were applied at the concentrations indicated in the respective tables.

Chicken tick  Result of relief effect test

The results are shown in Tables 1 to 8 below. ≪ tb > < TABLE >

In the table below, the rate of insecticides is calculated by the method of the number of dead chicken mites (ie, insecticides) / the number of chicken mites applied to the test * 100.

Treatment and treatment concentration (2000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Grapefruit seed extract 5 50 Grapefruit seed extract 4 Grapefruit seed extract 6 Average 5 tannin 0 0 tannin 0 tannin 0 Average 0 Chrysanthemum extract 30% + 5% of tea extract + sterilized water 65% -1 One 13 Chrysanthemum extract 30% + Extract of tea 5% + Sterile water 65% -2 2 Chrysanthemum Extract 30% + Tea Extract 5% + Sterile Water 65% -3 One Average 1.33 Abamectine One 10 Abamectine One Abamectine One Average One No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

Treatment and treatment concentration (2000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Emamectine benzoate < RTI ID = 0.0 > One 10 Emamectine benzoate < RTI ID = 0.0 > One Emamectine benzoate < RTI ID = 0.0 > One Average One Clove extract 10 97 Clove extract 9 Clove extract 10 Average 9.67 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

Treatment and treatment concentration (2000 ppm) Number of insects per 20 birds of chick mite Insecticidal rate (%) Tea extract 30% + Ethanol 30% + Sterile water 40% 4 23.35 Tea extract 30% + Ethanol 30% + Sterile water 40% 4 Tea extract 30% + Ethanol 30% + Sterile water 40% 6 Average 4.67 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0 Treatment and treatment concentration (4000 ppm) Number of insects per 20 birds of chick mite Insecticidal rate (%) Tea extract 30% + Ethanol 30% + Sterile water 40% 9 40 Tea extract 30% + Ethanol 30% + Sterile water 40% 15 Tea extract 30% + Ethanol 30% + Sterile water 40% 0 Average 8 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

Treatment and treatment concentration (4000 ppm) Number of insects per 20 birds of chick mite Insecticidal rate (%) Grapefruit seed extract 5 23.35 Grapefruit seed extract 6 Grapefruit seed extract 3 Average 4.67 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0 Treatment and treatment concentration (2000 ppm) Number of insects per 20 birds of chick mite Insecticidal rate (%) Clove seed extract 80% + Grapefruit seed extract 20% 20 100 Clove seed extract 80% + Grapefruit seed extract 20% 20 Clove seed extract 80% + Grapefruit seed extract 20% 20 Average 20 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

Treatment and treatment concentration (2000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract 70% + tea extract 5% + sterilized water 25% 10 100 Clove extract 70% + tea extract 5% + sterilized water 25% 10 Clove extract 70% + tea extract 5% + sterilized water 25% 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0 Treatment and treatment concentration (1000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract 70% + tea extract 5% + sterilized water 25% 10 100 Clove extract 70% + tea extract 5% + sterilized water 25% 10 Clove extract 70% + tea extract 5% + sterilized water 25% 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

Treatment and treatment concentration (2000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract 80% + sterilized water 20% 10 100 Clove extract 80% + sterilized water 20% 10 Clove extract 80% + sterilized water 20% 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0 Treatment and treatment concentration (1000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract 80% + sterilized water 20% 10 100 Clove extract 80% + sterilized water 20% 10 Clove extract 80% + sterilized water 20% 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

Treatment and treatment concentration (2000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract 50% + tea extract 5% + sterilized water 45% 10 100 Clove extract 50% + tea extract 5% + sterilized water 45% 10 Clove extract 50% + tea extract 5% + sterilized water 45% 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0 Treatment and treatment concentration (1000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract 50% + tea extract 5% + sterilized water 45% 10 100 Clove extract 50% + tea extract 5% + sterilized water 45% 10 Clove extract 50% + tea extract 5% + sterilized water 45% 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

Treatment and treatment concentration (2000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) 50% of clove extract + 50% of sterilized water 10 100 50% of clove extract + 50% of sterilized water 10 50% of clove extract + 50% of sterilized water 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0 Treatment and treatment concentration (1000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) 50% of clove extract + 50% of sterilized water 10 100 50% of clove extract + 50% of sterilized water 10 50% of clove extract + 50% of sterilized water 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

The results of the above formulation 10), a mixture of 80% by weight of the extract of clove, 10% by weight of polyethylene glycol, and 10% by weight of polyoxyethylenedidecyl monoether (a sample of clove extract formulation) Respectively.

Treatment and treatment concentration (1000 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract formulation formulation sample 10 100 Clove extract formulation formulation sample 10 Clove extract formulation formulation sample 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0 Treatment and treatment concentration (500 ppm) Number of insects per 10 cattle ticks Insecticidal rate (%) Clove extract formulation formulation sample 10 100 Clove extract formulation formulation sample 10 Clove extract formulation formulation sample 10 Average 10 No processing -1 0 0 No treatment -2 0 No treatment -3 0 Average 0

These results suggest that grapefruit seed extract, dried chrysanthemum extract, clove extract, and tea extract have different effects on chicken mite remedies. Especially, compared with abamectin and emamectin benzoate, which are applied to the chicken mite remedy using commercial chemical insecticides, the above extracts showed excellent chicken mite remedies even though they are natural extracts.

In the case of the grapefruit seed extract, the insecticidal rate was about 50% at the treatment concentration of 2000 ppm in the single treatment, and the insecticidal rate was not increased even at the treatment of 4000 ppm.

In the case of Chrysanthemum morifolium extract, the insecticidal rate was about 13% when treated with 2000 ppm of Chrysanthemum morifolium extract.

In the case of the clove extract, the effect of strong chicken mite remedy was shown to be about 10 times higher than that of the above-mentioned commercial insecticide. The concentration of 2000 ppm of the extract was effective as a mixture with grapefruit seed extract, And even when it is mixed with the solvent of the solvent. In addition, excellent results were also obtained in the formulated sample mixed with the surfactant component, and it was found that even when a low concentration of 500 ppm was applied, excellent results were obtained.

In the case of the tea extract, the insecticidal effect was exhibited by the mixture alone or with the solvent such as ethanol (or sterilized water), and the insecticidal effect was also excellent when mixed with the clove extract.

The above extracts are extracts of natural products, which are less toxic than chemical agents and are environmentally friendly and have excellent stability, which is highly applicable for the relief of chicken mites in poultry.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (6)

Wherein the extract is selected from the group consisting of grapefruit seed extract, dried chrysanthemum extract, chrysanthemum extract, clove extract, and combinations thereof as an active ingredient.
The method according to claim 1,
Wherein said veterinary composition comprises at least 50% by weight of clove extract as said natural product extract.
A method for relieving chicken mites in poultry, comprising the step of applying the veterinary composition according to claim 1 to a poultry or poultry house.
Grapefruit seed, chrysanthemum, chrysanthemum, clove, clove, and combinations thereof to prepare a natural product powder;
An extracting step of mixing the natural product powder with an extraction solvent to conduct an extraction process, and then removing a solid portion to obtain a crude extract; And
And a separating step of separating the crude extract into an oil layer and a water layer to obtain a natural oil-derived oil layer, thereby producing a natural extract comprising the natural oil-derived oil layer. ≪ / RTI >
5. The method of claim 4,
Wherein the extraction solvent is applied with hot water, and the natural product powder and the hot water are mixed at a weight ratio of 100: 20 to 100. The method for manufacturing a veterinary composition for relieving chicken mites,
5. The method of claim 4,
The veterinary medical composition for relieving chicken mites contains, based on the entire veterinary composition for relieving chicken mites, at least 50% by weight of an extract of cloves containing the above-mentioned clove-derived oil, Way.