KR20170141409A - Red mite acaricidal composition having natural plant extracts - Google Patents

Abstract

The present invention relates to an acaricide composition using a natural plant extract. More specifically, the present invention relates to an acaricide composition for Dermanyssus gallinae, containing a solution mixture of neem oil and a cold water-extract of ginkgo leaves as an active ingredient. The acaricide composition containing the neem oil and the cold water-extract of ginkgo leaves shows enhanced acaricide effects compared a case when a natural extract is solely applied. In addition, through an optimized composition of an antioxidant, vegetable oil, and a surfactant capable of increasing acaricide effects in the natural extract, it is possible to control Dermanyssus gallinae with the natural extract without using organic chemicals, thereby being eco-friendly and economically feasible.

Description

[Technical Field] The present invention relates to a red mite acaricidal composition having natural plant extracts,

The present invention relates to an acaricidal composition using a natural plant extract, and more particularly, to a chicken acaricidal composition comprising a mixed liquid of nematic oil and a cold extract of ginkgo biloba as an active ingredient.

With the recent introduction of the systemized cage structure, the daily management has been mechanized and the temperature and humidity environment inside the cage has become constant yearly. In this background, the proliferation of chicken mites (Dermanyssus gallinae) is proliferating irrespective of the season, and the damages of laying hens caused by chicken mite parasitism are increasing. Furthermore, the emergence of chicken ticks, which show resistance to drugs, and the exceptional use of licensed medicines make it difficult to control.

Chicken mite is an external parasite belonging to the mite family of mite, mid-morning moth, and it is parasitic to birds and sucks blood. It is difficult to find ticks that are parasitic on the chickens and bloodshed mainly because they are hidden at night and during the day when they are hidden in shaded areas or slits, and when they are noticed, they often occur in large quantities. After hatching, the young ticks are about 0.5 mm in length and have three pairs of legs. Adult ticks are 1 mm in length and 4 pairs in length. Immediately after acupuncture, adults have ticks that are more than 1 mm in diameter. The amount of blood per bird is very small, but on large farms, chickens may die of anemia.

Chicken mites become larval ticks in the eggs after hatching and become the first young ticks without the process of vomiting. The first young mite becomes the second young mite within 24 hours after the blood sucking, and then becomes more mature mite after blood sucking. In about 9 days from eggs, it becomes a mite tick and repeats vomiting and scattering.

Typical damages caused by chicken ticks are anemia caused by blood vomiting, mortality, low egg production due to stress, and the generation of pollutants caused by other tick excretions or blood-sucking ticks. In addition, the immune response to vaccination has been reported to be reduced by chicken parasitism. In the questionnaire surveys about the damage of chicken ticks in domestic poultry farms, the most frequent occurrence was contamination, followed by a decrease in spawning, anemia and loss of life, and mortality in the order of 56%. In addition, more than half of the respondents who had an effect on the worker, such as discomfort or allergy, accounted for more than half.

Chicken mites are frequently mediated by various bacterial and viral infections due to blood sucking. In developing countries, it has been reported that chicken mites are involved in multiple infectious diseases such as Salmonella infection and mononeutrient infection. Salmonella, which has been propagated in chicken ticks, has spread to the next generation through eggs. In Japan, the occurrence of phytopathogenic fungi in the chicken mite contaminated house was confirmed, and the phytophthora virus was detected from the chicken mite collected in the house. It is predicted that chicken pods have a history of pod vaccination, and that the stress caused by chicken parasitic influences may have an impact on the immune system.

In Korea, pyrethroid, carbamate, and organophosphorus are the most commonly used medicines to control chicken mites in Korea. Recently, phenylpyrazole-based fipronil fipronil) is also used. The domestic animal pharmaceuticals company has released a product called "Wagu Free" containing formic acid and 2-tert-butyl-5- (4-tert-butylbenzylthio) -4-chloropyridazin-3 (2H) Due to the tolerance, there is a need for a method for the preparation of bifenthrin (2-methyl [1,1'-biphenyl] -3-yl-methyl 3- (2-chloro-3,3,3-trifluoro- "On the other hand, in some laying hens farms, phytotoxic insecticides such as agricultural chemicals and organic chemicals are used indiscriminately. In other words, it is often the case that chick peat is replaced with an organic synthetic pesticide, which is currently used as a chemical agent or which suppresses insect pests of the crops in a negative manner. Therefore, harmful chemicals or pesticides may remain in the livestock products To consume them as may adversely affect public health.

Accordingly, the present inventors have made efforts to develop an acaricide composition for alleviating chick ticks in a more environmentally friendly and economical way in livestock farms. As a result, it has been found that Neem ( Azadirachta indica ) oil, Sophora fibratescens extract, Cinnamomum verum extract, and Ginkgo biloba extract showed a salicidal activity against chicken mites. When sprayed with a mixture of nymph oil and cold water extract of ginkgo biloba, Of the population. Also, by selecting the surfactant, vegetable oil and antioxidant composition capable of increasing the effect of using nem oil and cold water extract of Ginkgo biloba as an acaricide composition, the acetic acid composition of the present invention can be used as an acaricide composition And thus the present invention has been completed.

Accordingly, the present inventors have found that when the composition of surfactant, vegetable oil and antioxidant which can increase the effect of using nymph oil and ginkgo biloba cold water extract as an acaricide composition is selected and the optimum conditions are selected, And can be effectively used as an acaricide composition, thus completing the present invention.

Accordingly, an object of the present invention is to provide an acarbicide composition using an extract derived from a natural plant, excluding an organic compound.

In order to achieve the above object, the present invention's (Neem, Azadirachta indica ) oil, gosam ( Sophora flavescens extract, cinnamomum verum extract and a bank ( Ginkgo biloba ) extract as an active ingredient.

Specifically, the present invention provides an acaricidal composition comprising nematic oil and a ginkgo extract as an active ingredient.

In a preferred embodiment of the present invention, the extract may be extracted with water, C ₁ to C ₄ lower alcohol or a mixture thereof as a solvent, and the lower alcohol may be ethanol or methanol.

In another preferred embodiment of the present invention, the bank extract may be an extract obtained by cold extraction of gingko leaf.

In another preferred embodiment of the present invention, the nymph oil and the bank extract may be contained in an amount of 30 to 70% (v / v) parts by weight and 15 to 50% (v / v) parts by weight, respectively.

In another preferred embodiment of the present invention, the acaricidal composition is selected from the group consisting of methyl oleate, 2-dodecoxyethanol, polyoxyethylene monooleate or Tween 80 80) in a concentration of 2 to 10% (v / v) of the surfactant.

In another preferred embodiment of the present invention, the acaricide composition may further comprise a vegetable oil of any one of vegetable oil, castor oil, corn oil or edible oil at a concentration of 3 to 10% (v / v).

In another preferred embodiment of the present invention, the acaricide composition further comprises 50 to 100 ppm of an antioxidant selected from the group consisting of ethoxyquin, buylated hydroxyannisole (BHA), and buylated hydroxytoluene (BHT) Lt; / RTI >

In another preferred embodiment of the present invention, the acaricide may be at least one selected from the group consisting of house dust mites, white mites, chicken mites and ear mites.

Accordingly, the present invention provides a chick pea acaricide composition comprising nem oil and ginkgo biloba extract which are natural plant extracts.

The salicide composition comprising the nematic oil and ginkgo biloba extract of the present invention exhibits an increased salting effect compared with the case of spraying alone with the natural extract, and the surfactant, the vegetable oil and the antioxidant composition capable of enhancing the alleviating effect of the natural extract Because it is included as an optimal condition, it is possible to control against chicken ticks as a natural extract ingredient by eliminating organic chemicals, thereby being environmentally friendly and economical.

Fig. 1 shows the confirmation of the chick mite attack effect by the single administration of the plant extract.
Fig. 2 shows the confirmation of the chick lice sparing effect by mixed administration of plant extracts.
Figure 3 shows the wasting effect of chick peat acaricide compositions comprising nematic oil and ginkgo biloba extract.

Hereinafter, the present invention will be described in detail.

As described above, in the conventional method for salvaging chicken mites, there are many cases where organic chemicals are used or organic synthetic pesticide insecticides that suppress the pests of crops are used alternatively. Therefore, Since the pesticide ingredient may remain, it may adversely affect the public health to ingest.

The composition of the present invention can be used as an active ingredient in cosmetics, cosmetics, cosmetics, pharmaceuticals, cosmetics, pharmaceuticals, cosmetics, pharmaceuticals, It is environmentally friendly and economical because it is possible to control against chicken ticks as a natural extract ingredient by eliminating the agent.

Thus, the present invention is directed to < RTI ID = 0.0 > Neem, Azadirachta indica ) oil, Sophora flavescens extract, cinnamomum verum extract and a bank ( Ginkgo biloba ) extract as an active ingredient.

More specifically, the present invention provides an acaricidal composition comprising nematic oil and a bank extract as an active ingredient.

The "extract" of the present invention is preferably extracted with water, C ₁ to C ₄ lower alcohols or a mixture thereof as the solvent, and the lower alcohol is preferably ethanol, methanol or butanol.

The extract is preferably, but not exclusively, prepared by a process comprising the steps of:

1) extracting the plant by adding an extraction solvent;

2) filtering the extract of step 1); And

3) Concentrating the filtered extract of step 2) under reduced pressure and drying.

In the above method, the ginseng, bank or cinnamon of step 1) may be used without limitation such as cultivated or marketed. More preferably, the bank uses ginkgo biloba leaves, but the present invention is not limited thereto.

In the above method, the plant extract may be extracted by a conventional method such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction.

In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in step 3), but it is not limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but not always limited thereto.

The "acaricidal composition" of the present invention preferably contains a mixture of nymph oil and ginkgo biloba extract, specifically nymph oil at a concentration of 30 to 70% (v / v) and ginkgo biloba extract at 15 to 50% v / v) concentration, but the present invention is not limited thereto.

The "salicide composition" of the present invention preferably contains a surfactant for mixing the water layer and the oil layer, since it contains nem oil as a lipophilic component and water extract of ginkgo biloba as a water-soluble component. Specifically, the surfactant is preferably one selected from the group consisting of methyl oleate, 2-dodecoxyethanol, polyoxyethylene monooleate, and Tween 80 More specifically, the surfactant is preferably Tween 80, but it is not limited thereto. The surfactant is harmless to the human body and is eco-friendly, and can improve the stability and ease of use of the present acarbicide composition. Any active agent can be used.

The surfactant is preferably contained at a concentration of 2 to 10% (v / v), more preferably at a concentration of 7.5 to 10% (v / v) When the layer is mixed, it is preferable that the concentration is such that the emulsifying property can be maintained. If the surfactant is contained in an amount of less than 2% (v / v), emulsification of the water and oil layers is not carried out. If the surfactant is contained at a concentration exceeding 10% (v / v) It is not effective.

The " acaricidal composition " of the present invention includes an oil layer, so that when applied to a subject livestock containing chicken, it is possible to spread the water surface to the livestock surface effectively, As shown in Fig. Specifically, the vegetable oil is preferably any one of vegetable oils, castor oils, corn oil, and edible oils. More specifically, the vegetable oil is more preferably a vegetable oil, but is not limited thereto. The vegetable oil may be inexpensively and readily available. Any of those known in the art may be used.

The vegetable oil is preferably contained at a concentration of 3 to 10% (v / v), more preferably at a concentration of 3 to 5% (v / v), but is not limited thereto. It is preferable to appropriately use a concentration capable of promoting the growth of the particles.

An antioxidant capable of preventing the oxidation of the oil layer in order to maintain physicochemical stability and continuous insecticidal effect after the oil layer contained in the " acarbicide composition " of the present invention is sprayed as an acaricide and exposed to air is further added . The above-mentioned acaricide composition is preferably one of ethoxyquin, buylated hydroxyannisole (BHA), and buylated hydroxytoluene (BHT), and more preferably ethoxyquinone.

The antioxidant is preferably contained in a concentration of 50 to 100 ppm, more preferably 60 to 80 ppm. When an antioxidant is added to the acarbicide composition of the present invention at an amount of less than 50 ppm, the development of the water surface is not preferable and is not economical.

The "acaricide composition" of the present invention is preferably an acaricide for at least one selected from the group consisting of house dust mite, white mite, chick mite and ear mite, and more preferably an acaricide composition for control of chick mite, It is not limited.

In a specific example of the present invention, the present inventors purchased and prepared a plant extract known to have an insecticidal effect for use as an acaricide composition, and purchased or manufactured nymph oil, gossam extract, ginkgo leaf extract and cinnamon extract, (Table 1 and Fig. 1). When the extracts of Nymph oil and Ginkgo biloba were mixed at a ratio of 60:40 (v / v), the chick mite (Fig. 2 and Fig. 2). The results are shown in Table 2 and Fig. 2, respectively. ≪ tb > < TABLE >

The inventors of the present invention found that when using a mixture of nymph oil and ginkgo biloba extract as an active ingredient of the acaricide composition, the surfactant and the adjuvant component were selected to enhance the anti-aging effect and the optimum concentration was determined. As a result, % Vegetable oil and 75 ppm ethoxyquinone, it was confirmed that the water and oil layers were effectively mixed with the acetic acid to improve the emulsification property and the best development of the water surface (Table 3 and Table 4).

In addition, the present inventors prepared a prototype by mixing a mixture of nymph oil and ginkgo biloba extract and an adjuvant component (Table 5), and found that when it was applied at a dilution ratio of 1/100 to 1/250 It was confirmed that Salvy activity against chicken mite was significant (Table 6 and Fig. 3).

Therefore, the mixed liquid of nymph oil and ginkgo biloba extract of the present invention exhibits an increased salivation effect on chicken mites compared with the case of spraying natural extracts alone, and can be used as a surfactant, vegetable oil, Since the composition contains an antioxidant composition as an optimal condition, it is possible to control against chicken ticks as a natural extract ingredient by eliminating an organic chemical, thereby being environmentally friendly and economical.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

Manufacture of plant extracts

Plant extracts known to have an insecticidal effect for use as an acaricide composition in the present invention were purchased and prepared.

Specifically, you (Neem, Azadirachta indica ) Extract and Sophora flavescens ) extracts were commercialized. Cinnamomum verum ) and ginkgo leaf (Gingko) were prepared by using water, 50% ethanol aqueous solution or 100% ethanol as extraction solvent. In the case of cinnamon, 25 g of cinnamon was sliced into 75 ml of extraction solvent of water, 50% ethanol solution or 100% ethanol, and extracted at room temperature for 7 days. The ginkgo leaf was pulverized after grinding, and 40 g of Ginkgo biloba was immersed in 200 ml of extraction solvent in the extraction container and extracted at room temperature for 7 days. The extract was filtered to remove debris, further centrifuged and the supernatant was recovered as a plant extract. Finally, the extracted extracts were refrigerated until they were tested for saliva.

Plant extract Salvi  Check the effect

≪ 2-1 > Chicken tick Salvi  Check the effect

The plant extracts were sprayed on the chicken mites to confirm the killing effect of the chicken mites on the chicken mites.

Specifically, a large amount of chicken mites were collected from the laying hens' farms, and the population was maintained at 30 ° C and 60% relative humidity until the experiment. Then sterile filter paper was placed on a plant culture plate with a width of 63.5 cm ² , the plate was covered with a plate lid so that the tick could breathe, and the plate was finely finished to prepare a culture plate. 100 horses each of which kept the above population were separated from the prepared plates. After separation, the plant extracts purchased or manufactured in Example 1 were diluted to a concentration of 1/100 and sprayed uniformly with 2 ml onto each plate using an atomizer. Twenty-four hours after spraying, the ticks were touched by a thin saliva and the non-moving individuals were regarded as dead ticks. As a negative control, water was sprayed. As a positive control, 100% ethanol was sandwiched. Salvation test was repeated three times.

As a result, as shown in Table 1 and Fig. 1 below, it was confirmed that nimoo oil had the best anti-alleviative effect against chicken germ (Table 1 and Fig. 1).

By plant extract alone Chicken tick Salvi  Check the effect Plant extract Average mortality (%) Negative control group  9.6 Positive control group 11.6 Nem oil 74.3 Gosam extract 42.3
Ginkgo leaf
Water extract 60.3 50% ethanol aqueous solution extract 39.5 100% ethanol extract 55.3
cinnamon
Water extract 52.3 50% ethanol aqueous solution extract 34.7 100% ethanol extract 49.3

≪ 2-2 > Chicken tick Salvi  Check the effect

Since each plant extract was found to exhibit a chlamydial effect against chicken mites, the extracts exhibiting an average mortality rate of 50% or more were mixed and sprayed with plant extracts on chicken mites to confirm the death rate of chicken mites.

Specifically, the mixture of extracts was prepared by mixing 40% of each of water extract of gingko leaf, 100% ethanol extract of bankamen or water extract of cinnamon, based on 60% of nymph oil. 100% nematic oil was used as a no-added control, and the same method as in Example <2-1> was used to spray on chicken mites and the cholesty effect was confirmed.

As a result, as shown in FIG. 2 and the following Table 2, it was confirmed that the composition of acetic acid mixed with nymph oil and ginkgo biloba extract was more effective than the case of treating nymph oil alone (FIG. 2 and Table 2).

Extract mixture Average mortality (%) Nymph oil 100% 65.4
Nymph oil 60%
Ginkgo leaf water extract 40% 70.2 Ginkgo leaf ethanol extract 40% 56.7 Cinnamon water extract 40% 53.1

Acarbicide  Screening of surfactant and adjuvant ingredients in the composition

<3-1> Emulsification test

In order to formulate a biological material as an acaricide composition, it is necessary to use a certain surfactant in order to stabilize physical properties of the product and convenience in use. Since these surfactants are harmless to the human body and environmentally friendly materials are required, the degree of emulsification of the extract mixture is confirmed in the raw materials with safety.

Specifically, candidate substances include methyl oleate, 2-dodecoxyethanol, polyoxyethylene monooleate, tween 80, and the like, which are surfactants corresponding to EPA lists 4A and 4B, (Tween 80) was used. Then, each surfactant was added at a concentration of 1.0%, 2.5%, 5.0%, 7.5%, or 10.0% to a mixed solution of 60% of nimrofen and 40% of ginkgo biloba extract and mixed for 24 hours. After 24 hours, the emulsifiability was visually observed, and the results were judged by dividing into excellent (+++) good (++), normal (+) and bad (-).

As a result, it was confirmed that the emulsifying property of Tween 80 was the best as shown in Table 3 below, and that it was appropriate to treat at a concentration of 2.5% or more (Table 3).

Acarbicide  Screening of Surfactants in the Composition division EPA lsit Addition concentration (%) One 2.5 5 7.5 10  Methyl oleate 4B - - + ++ ++  2-dodecoxyethanol 4B - - + + ++  Polyethylene monooleate 4B - - + ++ +++  Twin 80 4B + ++ ++ +++ +++

<3-2> Vegetable oil test to improve sleep development pattern

In order to improve the development of the water surface and to select the adjuvant for increasing the cholesterol efficacy while using the liposoluble layer containing nematic oil as an acaricide composition, the vegetative oil was added at different concentrations to compare the surface development patterns of the main components.

Specifically, the vegetable oil of the candidate substance was seed oil, castor oil, corn oil or edible oil. Then, each vegetable oil was added to nimrod oil at a concentration of 1%, 2%, 4% or 8%, and the development of the water surface was observed for 20 days. At the time of observation, it was judged by dividing into four stages of excellent (+++) good (++), normal (+) and bad (-).

As a result, as shown in Table 4, vegetable oils of seed oil, castor oil, corn oil and edible oil showed the best physical properties when added at a concentration of 4%. Among them, Respectively. Finally, we selected edible oils that are easy to purchase and low in cost (Table 4).

Acarbicide  Selection of vegetable oils contained in the composition Input rate
Elapsed time (days)
vegetable oil
Seed oil Pharma Liberty Corn oil cooking oil One%


5 +++ ++ +++ +++ 10 ++ ++ ++ ++ 15 ++ ++ + ++ 20 + + + + 2%


5 +++ +++ +++ +++ 10 ++ ++ ++ +++ 15 ++ ++ + ++ 20 + + + + 4%


5 +++ +++ +++ +++ 10 ++ ++ ++ +++ 15 ++ ++ ++ ++ 20 ++ + + ++ 8%


5 +++ +++ +++ +++ 10 ++ ++ ++ ++ 15 + + + ++ 20 + + + +

<3-3> Selection of antioxidants to improve surface development

It is important to maintain physicochemical stability and persistent insecticidal effect when it is sprayed as an acaricide and exposed to air because the effective ingredient is a vegetable oil. To this end, an antioxidant capable of securing the safety of the effective ingredient is selected Respectively.

Specifically, candidate substances were ethoxyquin, buylated hydroxyannisole (BHA), or buylated hydroxytoluene (BHT), which are antioxidants used for food and livestock feed additions. Then, each antioxidant was added to the mixture of nymphenic acid and ginkgo biloba extract at a ratio of 1: 1 (v: v) at a concentration of 25, 50, 75 or 100 ppm, Respectively.

As a result, no significant difference was observed according to the concentration of the antioxidant, and it was confirmed that when the ethoxyquin was treated at 75 ppm, the development of the water surface was most favorable after 20 days. In addition, ethoxyquinone is a substance that can be added to feed, and since it is more economical than BHA or BHT, it is advantageous to ultimately select ethoxyquin.

Containing nymph oil and ginkgo biloba extract Chicken tick Acarbicide  Preparation of composition

An acarbicide composition comprising the selected and selected ingredients in Example 2 and Example 3 was prepared.

Specifically, the composition of the following [Table 5] was prepared, and the water extract of Ginkgo biloba and glycerin were mixed in a mixer for about 5 minutes to prepare a receptive layer. Then, nymph oil, edible oil, ethoxy queen, and tween 80 were added to the mixer in order and mixed for about 10 minutes to prepare an oil layer. The prepared oil layer was added to the homogenizer, the homogenizer was operated and the receptive layer was added, and the prototype was prepared by emulsifying for 20 minutes. The salvage effect on the stored prototype was the same as in Example <2-1>, and the prototype was diluted and sprayed at 1/100, 1/250, 1/500 or 1/1000. As a control, nim oil not mixed with the composition was sprayed and used.

Containing nymph oil and ginkgo biloba extract Chicken tick Acarbicide  Composition of composition Ingredients Addition ratio (V / V,%) Ginkgo biloba water extract  35 glycerin   4 Nem oil 52 cooking oil   4 Ethoxy queen        0.0075 Twin 80   5 Sum 100

As a result, as shown in Fig. 3 and the following Table 6, it was confirmed that the emulsion composition layer was not separated even after 3 months from the production of the prototype, and the salivary activity against chicken ticks was 1/100 to 1 / 250 was considered to be most appropriate.

Containing nymph oil and ginkgo biloba extract Chicken tick Acarbicide  Of the composition Salvia effect Dilution factor Average
Lethality (%) Control group  7.3 1/100 64.9 1/250 49.4 1/500 27.6 1/1000 14.2

Claims (11)

By Neem, Azadirachta indica ) oil, gosam ( Sophora a cinnamomum verum extract, and a bank extract ( Ginkgo biloba ) as an active ingredient.
The acaricidal composition according to claim 1, wherein the extract is extracted with water, C ₁ to C ₄ lower alcohols or a mixture thereof as a solvent.
The acaricide composition of claim 2, wherein the lower alcohol is ethanol or methanol.
The acaricidal composition according to claim 1, wherein the acaricide is at least one acaricide selected from the group consisting of house dust mites, white mites, chicken mites and ear mites.
Nematic oil and ginkgo extract as active ingredients.
The acaricide composition according to claim 5, wherein the bank extract is an extract obtained by cold extraction of ginkgo leaves.
6. The composition of claim 5, wherein the nymph oil and the bank extract are contained in an amount of 30 to 70% (v / v) parts by weight and 15 to 50% (v / v) parts by weight, respectively.
6. The composition of claim 5, wherein the acaricide composition is selected from the group consisting of methyl oleate, 2-dodecoxyethanol, polyoxyethylene monooleate, or Tween 80 Of a surfactant at a concentration of 2 to 10% (v / v).
The acaricidal composition according to claim 5, wherein the acaricidal composition further comprises a vegetable oil of any one of vegetable oil, castor oil, corn oil or edible oil at a concentration of 3 to 10% (v / v).
6. The composition of claim 5, wherein the acaricide composition further comprises 50 to 100 ppm of an antioxidant selected from the group consisting of ethoxyquin, buylated hydroxyannisole (BHA), and buylated hydroxytoluene (BHT). &Lt; / RTI &gt;
The acaricidal composition according to claim 5, wherein the acaricide is acaricide for at least one selected from the group consisting of house dust mite, white mite, chicken mite and ear mite.

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