KR102202303B1 - Insecticide composition using ginkgo leaves and method for manufacturing the same - Google Patents

Abstract

The present invention relates to an insecticide composition using ginkgo leaves and a method for preparing the same, and more particularly, to an insecticide composition using ginkgo leaves, which has a high sterilization and insecticidal effect and has removed the peculiar smell of ginkgo leaves, and to a method for preparing the same. The insecticide composition using ginkgo leaves of the present invention includes a ginkgo leaf extract, wherein the ginkgo leaf extract is prepared by mixing ginkgo leaf powder with distilled water, followed by double boiling and filtration.

Description

Insecticide composition using ginkgo leaves and method for manufacturing the same}

The present invention relates to an insecticide composition using ginkgo biloba and a method for manufacturing the same, and more particularly, to an insecticide composition using ginkgo biloba leaves having a high bactericidal insecticidal effect and a odor peculiar to gingko leaves, and a method of manufacturing the same.

In modern agriculture, synthetic pesticides are used for pest control. Pesticides have contributed greatly to increasing agricultural productivity and protecting humans from pests, and their use has been steadily increasing. Currently, pesticides such as pesticides and herbicides are mainly organic chlorine, organic phosphoric, and carbamate, all of which have the effect of being concentrated by the food chain in the ecosystem due to severe toxicity and low degradability.

The disadvantage of these chemically-synthetic pesticides is that the pest control effect is gradually reduced due to the emergence of resistant pests due to the increase in the number of times of application of the drug and the increase in the concentration of application, while the natural enemies are lethal and become pests of potential insects. The emergence of new pests is increasing. In addition, the increase in the amount of residual pesticides in agricultural products increases the toxicity of agricultural products, acting as a negative factor for food.

Biological control is being actively studied as part of the development of next-generation pesticides to overcome the limitations of synthetic pesticides. Biological control uses microorganisms, natural enemies and biochemicals extracted from natural products. In the case of microbial preparations and natural enemies, they are difficult to handle, which is an obstacle to commercialization. Therefore, there is a need for the development of natural pesticides that are harmless to humans and animals and plants within a faster time, which are environmentally friendly, easy to handle, and capable of developing products with low production prices.

On the other hand, the ginkgo tree is highly resistant to all pests and diseases, and is evaluated as a plant with high resistance to industrial pollution such as urban smoke.

Conventionally, a technology for producing an insecticidal ginkgo leaf extract by mixing ginkgo leaves with distilled water or alcohol has been introduced, but using only ginkgo leaf extract may have little insecticidal effect or cause weak damage to crops. There is a problem that can cause rejection.

Korean Patent Publication No. 10-2002-0070598

In order to solve the problems of the prior art as described above, it is an object of the present invention to provide an insecticide composition using gingko leaves and a method for producing the same, which contains gingko leaves and an active substance to reduce off-flavor, and has excellent sterilization and insecticidal effects.

The technical problems to be solved by the invention are not limited to the technical problems mentioned above, and other technical problems that are not mentioned will be clearly understood by those of ordinary skill in the technical field to which the present invention belongs from the following description. I will be able to.

Insecticide composition using ginkgo biloba of the present invention comprises a ginkgo leaf extract, wherein the ginkgo leaf extract is characterized in that it is prepared by mixing ginkgo leaf powder with distilled water and then boiling water and then filtering.

The pesticide composition using ginkgo biloba of the present invention includes a ginkgo leaf concentrate and a nonionic surfactant, wherein the ginkgo leaf concentrate is obtained by vacuum concentration after immersing ginkgo leaf powder in methanol, and the nonionic surfactant is polyso It is characterized in that the bait 20 (Polysorbate 20), polysorbate 80 (Polysorbate 80).

Insecticide composition using ginkgo biloba of the present invention includes ginkgo biloba extract, amino acids or N-acetyl amino acids, and sugars, and the ginkgo biloba extract is prepared by mixing ginkgo leaf powder with distilled water and then boiling water, followed by filtration, and the amino acid or N -Acetyl amino acid is at least one of methionine, cysteine, cystine, N-acetyl cysteine, N-acetyl methionine, and N-acetyl proline, and the saccharide is at least any one of fructose, glucose, sucrose, maltose, and xylose It is characterized by being.

The method for preparing an insecticide composition using ginkgo leaves of the present invention comprises: a first step of mixing ginkgo leaf powder with distilled water, boiling water, and then filtering to prepare a ginkgo leaf extract; A second step of preparing a mixture by adding amino acids, N-acetyl amino acids, and saccharides to the gingko leaf extract; A third step of stirring and heating the prepared mixture; A fourth step of adjusting the heated mixture to pH 3 to 6; including, wherein the amino acid or N-acetyl amino acid is methionine, cysteine, cystine, N-acetyl cysteine, N-acetyl methionine, N-acetyl proline It is at least any one, and the saccharide is at least one of fructose, glucose, sucrose, maltose, and xylose, and the pH adjustment is characterized by using at least one of citric acid, wood vinegar, and brown rice vinegar. .

Insecticide composition using ginkgo biloba of the present invention includes ginkgo biloba extract, vegetable extract, vegetable oil, plant extract, wherein the vegetable extract is at least one of pork potato extract, garlic extract, cheongyang pepper extract, onion extract, and the vegetable oil Is at least one of clovebird oil, lavender oil, rosemary oil, thyme white oil, cottonseed oil, lemon eucalyptus oil, peppermint oil, marjoram oil, canola oil, oregano oil, cinnamon bark oil, and the plant extract is nicotine extract It is characterized in that at least any one of, gosam extract, pyrethrum extract, neem extract.

By means of solving the above problems, the pesticide composition using ginkgo biloba of the present invention and a method for producing the same contain ginkgo leaves and an effective substance to reduce off-flavor, and provide an insecticidal composition having excellent sterilization and insecticidal effects.

In addition, there is an effect of providing a pesticide composition that can be used in various ways, such as indoor gardens, agricultural pests, and sanitary pests as eco-friendly pesticides.

In addition, there is an effect of providing an insecticide composition that has excellent sterilization and insecticidal effects through the reaction between the organic acid and Maillard, removes off-flavor peculiar to ginkgo biloba and gives a fragrance to increase consumer preference.

1 is a flow chart showing a method of manufacturing an insecticide composition using ginkgo leaves of the present invention.

Specific matters, including the problems to be solved, means for solving the problems, and effects of the invention as described above, are included in the following examples and drawings. Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail together with the accompanying drawings.

In the following, an insecticide composition using the ginkgo leaf and a method of manufacturing the same will be described with reference to drawings and examples.

<First Example>

The pesticide composition of the first embodiment comprises a ginkgo leaf extract.

The pesticide composition of the first embodiment includes a ginkgo leaf extract prepared by mixing ginkgo leaf powder with distilled water, boiling, and filtering.

More specifically, after mixing 9 to 50% by weight of ginkgo leaf powder and 50 to 91% by weight of distilled water, the mixture is bathed at 50 to 80°C for 2 to 5 hours, and then filtered to prepare an extract.

If the ginkgo leaf powder is mixed with less than 9% by weight, the content of the flavonoid component as an active ingredient may be excessively low, and if it exceeds 50% by weight, the solvent for extraction may be insufficient or a problem of evaporation of the active ingredient during the bath may occur. .

If the bathing time is less than 2 hours, the extraction of the active ingredient may not be sufficiently performed, and if it exceeds 5 hours, the extraction is already completed and the efficiency of the operation may decrease.

The prepared ginkgo leaf extract contains flavone glycoside, terpene lactone, and zinc cholic acid as main components.

In addition, the ginkgo leaf extract contains the main component, that is, 1 part by weight of at least one of ethanol, methanol, and n-heptane in a solution extracted with a brownish green solution by boiling ginkgo leaves at 60 to 100°C for 1 to 30 minutes It can be replaced with a solution extracted by mixing or a solution extracted by distilling under reduced pressure between 0.8X103 Pa and 1X102 Pa.

<Second Example>

The pesticide composition of the second embodiment comprises a ginkgo leaf concentrate and a nonionic surfactant.

The pesticide composition of the second embodiment includes a ginkgo leaf concentrate obtained by immersing ginkgo leaf powder in methanol and then concentrating in a vacuum and a nonionic surfactant.

As the nonionic surfactant, it is preferable to use Polysorbate 20 and Polysorbate 80.

The nonionic surfactant increases the insecticidal and sterilizing effect by maintaining excellent deposition and adsorption of the pesticide composition.

More specifically, 9 to 50% by weight of ginkgo leaf powder is added to 50 to 91% by weight of methanol, immersed for 5 to 10 days, and then vacuum concentrated to prepare a concentrated solution. Next, it is prepared by mixing 90% by weight of a nonionic surfactant to 10% by weight of the concentrate.

When the ginkgo leaf powder is mixed in less than 9% by weight, the content of the flavonoid component as an active ingredient may be excessively decreased, and when it exceeds 50% by weight, the extraction efficiency of the active ingredient may be rapidly lowered.

In addition, when the nonionic surfactant is mixed in less than 90% by weight, the effect of allowing deposition and adsorption with the target (plant, etc.) may not be sufficiently expressed. When it exceeds 90% by weight, the concentrate As the addition ratio is lowered, the insecticidal and sterilizing effects may be lowered, or safety problems may occur due to excessive use.

<Third Example>

The pesticide composition of the third embodiment comprises ginkgo leaf concentrate, amino acids or N-acetyl amino acids, and sugars.

The pesticide composition of the third embodiment includes a ginkgo leaf extract prepared by mixing ginkgo leaf powder with distilled water, boiling, and filtering, and a sulfur-containing amino acid or N-acetyl amino acid, and a saccharide.

The amino acid or N-acetyl amino acid is preferably at least one of methionine, cysteine, cystine, N-acetyl cysteine, N-acetyl methionine, and N-acetyl proline.

In addition, the saccharide is preferably at least one of fructose, glucose, sucrose, maltose, and xylose.

The pesticide composition of the third embodiment is prepared by heating and adjusting pH.

In more detail, it follows the method for preparing a ginkgo leaf insecticide composition as shown in FIG. 1.

1 is a flow chart showing a method for preparing an insecticide composition using ginkgo leaves according to a third embodiment of the present invention.

First, in the first step (S10), ginkgo leaf powder is mixed with distilled water and bathed, followed by filtering to prepare a ginkgo leaf extract.

Specifically, after mixing 9 to 50% by weight of ginkgo leaf powder and 50 to 91% by weight of distilled water, the mixture is bathed at 50 to 80°C for 2 to 5 hours, and then filtered to prepare an extract.

Next, in the second step (S20), an amino acid or N-acetyl amino acid, and a saccharide are added to the gingko leaf extract to prepare a mixture.

Specifically, a mixture is prepared by mixing 97 to 99% by weight of the prepared extract, 0.3 to 1% by weight of at least one of amino acids or N-acetyl amino acids, and 0.7 to 2% by weight of saccharides.

The amino acid or N-acetyl amino acid is preferably at least one of methionine, cysteine, cystine, N-acetyl cysteine, N-acetyl methionine, and N-acetyl proline.

The saccharide is preferably at least one of fructose, glucose, sucrose, maltose, and xylose.

The mixing ratio of the extract and amino acids or N-acetyl amino acids and saccharides is preferably carried out within the ranges presented above in order to smoothly perform the Maillard reaction and sufficiently exhibit the effects resulting therefrom.

Next, in the third step (S30), the mixture is stirred and heated.

Specifically, the mixture is first heated at 80 to 110° C. for 2 hours, and then stirred at 40 to 55° C. for 10 hours, followed by secondary heating.

The Maillard reaction proceeds through the first heating, and the reaction is stabilized and aged through the second heating.

When the first heating is less than 80° C., the reaction time is excessively long, and when it exceeds 110° C., the active ingredient may be lost, deteriorated, or boiled out.

When the secondary heating is less than 40° C., the stabilization and fragrance enhancement effect is lowered, and when it exceeds 55° C., a burning odor occurs, which may add off-flavor.

Next, in the fourth step (S40), the heated mixture is adjusted to pH 3 to 6.

The pH adjustment is preferably at least one of citric acid, wood vinegar, and brown rice vinegar. Through the pH adjustment, the acidity is preferably applied (absorption, safety, etc.) to be applied as an insecticide used in plants and the like.

Due to the mixing and heating of the amino group and sugar, off-flavor of the pesticide can be reduced through the Maillard reaction, and consumer preference can be improved through the fragrance and brown shape generated through the Maillard reaction.

<Fourth Example>

The pesticide composition of the fourth embodiment comprises a ginkgo leaf extract, a vegetable extract, a vegetable oil, and a plant extract.

The pesticide composition of the fourth embodiment includes a ginkgo leaf extract, a vegetable extract, a vegetable oil, and a plant extract, prepared by mixing ginkgo leaf powder with distilled water, boiling, and filtering.

The vegetable extract is preferably at least one of pork potato extract, garlic extract, green pepper extract, and onion extract.

In addition, the vegetable oil is preferably at least one of clovebird oil, lavender oil, rosemary oil, thyme white oil, cotton seed oil, lemon eucalyptus oil, peppermint oil, marjoram oil, canola oil, oregano oil, cinnamon bark oil. Do.

In addition, the plant extract is preferably at least one of nicotine extract (cigarette leaf extract), gosam extract, pyrethrum extract, and neem extract.

The vegetable extract, vegetable oil, and plant extract are mixed to deliver eco-friendly active ingredients (insecticide, sterilization, aroma) contained in vegetables and plants, and add the scent of vegetable oil to increase the insecticidal and sterilizing effect of the pesticide and enhance the fragrance. Can be given.

More specifically, after mixing 9 to 50% by weight of ginkgo leaf powder and 50 to 91% by weight of distilled water, the mixture is bathed at 50 to 80°C for 2 to 5 hours, and then filtered to prepare an extract. Next, it is prepared by mixing 70 to 91% by weight of the extract, 3 to 10% by weight of vegetable extract, 3 to 10% by weight of vegetable oil, and 3 to 10% by weight of plant extract.

The mixing ratio of the extract and vegetable extract, vegetable oil, and plant extract is preferably carried out within the range given above in order to impart the effect and fragrance of the active ingredient.

<Fifth Example>

The pesticide composition of the fifth embodiment includes the pesticide composition of the third embodiment, an amino acid or N-acetyl amino acid, a saccharide, and a vegetable extract, vegetable oil, and plant extract.

More specifically, it is prepared by mixing 70 to 91% by weight of the pesticide composition of the third embodiment, 3 to 10% by weight of vegetable extracts, 3 to 10% by weight of vegetable oil, and 3 to 10% by weight of plant extract.

The vegetable extract, vegetable oil, and plant extract are configured in the same manner as in the fourth embodiment.

The mixing ratio of the pesticide composition and vegetable extracts, vegetable oils, and plant extracts is preferably carried out within the ranges presented above in order to impart the effect and fragrance of the active ingredient.

The pesticide composition using ginkgo leaves can be variously applied according to the purpose of the user, such as a method of dissolving or diluting in water, distilled water, or spraying.

In addition, it may be used by mixing with additives such as separate active ingredients such as fertilizers or detergents or excipients, and may be used by adding a colorant, a surfactant, or an ark agent.

In addition, it can be used in various ways by changing the formulation such as powdering, pelletizing, granulating, and tableting using the pesticide composition.

The application amount of the pesticide composition can be changed in design depending on the purpose of use, application form, application site, application object, etc., and since the ginkgo leaf pesticide composition of the present invention is a natural component, it can be used in various amounts.

In the following, experiments were conducted to explain the effect of the pesticide composition using ginkgo biloba of the present invention, and the contents of the experiment and the results thereof will be described in detail.

In this experiment, after collecting blue ginkgo leaves from street trees in the city from September to November, 2019, ginkgo leaf powder that has been well-dried (dark air-dried for more than 1 week) is finely crushed (500㎛ or less) is used.

Example 1-1. After mixing 100g of ginkgo leaf powder and 1L of distilled water, bathing at 80℃ for 2 hours, and filtering through natural filtration method (filter paper NO.2 Toyo Roshi) to prepare ginkgo leaf extract.

Example 2-1. 100 g of ginkgo leaf powder was added to 1 L of methanol and immersed for 7 days, followed by mixing 10% by weight of the obtained concentrate and 90% by weight of 1% Tween 20 (TW-20) using a vacuum concentrator.

Example 3-1. Into 1 L of ginkgo biloba extract (extract of Example 1-1) 4 g of methionine and 8 g of xylose were added, maintained at 80 to 110°C and heated for 2 hours, followed by stirring and heating while maintaining at 40 to 55°C, Manufactured by adjusting pH to 4.5 with vinegar.

Example 3-2. In 1 L of ginkgo leaf extract (extract of Example 1-1), 8 g of cysteine and 8 g of xylose were added, and after heating for 2 hours while maintaining 80 to 110°C, stirring and heating were performed while maintaining 40 to 55°C, Manufactured by adjusting pH to 4.5 with vinegar.

Example 4-1. In 1 L of ginkgo biloba extract (extract of Example 1-1), 25 g of pork potato extract, 25 g of garlic extract, 25 g of green pepper extract, 25 g of onion extract, 20 of clovebird oil, 20 g of lavender oil, 20 g of rosemary oil, 20 g of thyme white oil, cotton Manufactured by mixing seed oil 20g, nicotine extract 25g, gosam extract 25g, pyrethrum extract 25g, neem extract 25g.

Example 5-1. In 1 L of ginkgo biloba extract (extract of Example 3-1), 25g of pork potato extract, 25g of garlic extract, 25g of green pepper extract, 25g of onion extract, 20g of clovebird oil, 20g of lavender oil, 20g of rosemary oil, 20g of thyme white oil, cotton Manufactured by mixing seed oil 20g, nicotine extract 25g, gosam extract 25g, pyrethrum extract 25g, neem extract 25g.

<Insecticide comparison experiment>

Taking the apple leaves of the ice-gol apple tree in Miryang Sannae-myeon, 20 spotted mites per leaf were inoculated on the back of the leaf, and 200 parts by weight of distilled water was added to 1 part by weight of the composition of Examples 1-1 to 5-1, and diluted. Then, each 10ml was sprayed using a small sprayer.

After 2 days of spraying, the number of spotted mites remaining on the leaves of apple trees was investigated and recorded for each leaf under a magnifying glass, and the number of individuals was investigated every 2 days, and the numbers were shown in Table 1.

(Control: No sample is processed)

division Mite population (mari) Day Day+2 Day+4 Average Sum average Example 1-1-a 16 13 11 13.33
14.77 Example 1-1-b 18 16 16 16.67 Example 1-1-c 16 15 12 14.33 Example 2-1-a 17 14 13 14.78
13.89 Example 2-1-b 16 16 10 14.00 Example 2-1-c 15 15 9 13.00 Example 3-1-a 15 9 6 10.00
9.11 Example 3-1-b 13 8 7 9.33 Example 3-1-c 12 7 5 8.00 Example 3-2-a 13 8 5 8.67
8.67 Example 3-2-b 15 8 6 9.67 Example 3-3-c 12 7 4 7.67 Example 4-1-a 16 14 11 13.67
14.78 Example 4-1-b 18 16 14 16.00 Example 4-1-c 17 15 12 14.67 Example 5-1-a 17 12 9 13.00
11.66 Example 5-1-b 16 13 8 12.33 Example 5-1-c 12 10 8 10 Control 17 15 14 15.33 15.33

As shown in Table 1, compared to the control (Comparative Example), Examples 1 to 5, which are insecticide compositions using ginkgo leaves of the present invention, exhibited an overall insecticidal effect.

In addition, in the case of Example 3 (Example 3-1, Example 3-2), since it can be confirmed that the number of mite populations has been significantly reduced, it can be seen that the highest insecticidal effect is exhibited.

<Scent comparison experiment>

In order to compare the fragrance characteristics of the pesticide composition using ginkgo biloba, graduate students of the College of Agriculture and Life Sciences who were recognized for their suitability as inspectors were selected, and after training appropriate for the purpose of this experiment, evaluation was conducted. As for the evaluation items, a 9 point scale (Liker scale) was used to evaluate the scent and irritating odor with 9 points for very good and 1 point for very bad, and these are shown in Table 2.

division Scent Irritating odor ranking Example 1-1 2 6 5 Example 2-1 2 5 6 Example 3-1 7 9 3 Example 3-2 8 9 2 Example 4-1 7 7 4 Example 5-1 9 9 One

As shown in Table 2, Examples 3 to 5 showed overall excellent sensory properties. Accordingly, it can be seen that the off-flavor peculiar to ginkgo leaves is removed through the reaction between the organic acid and the Maillard, and the preference of consumers is increased by giving a fragrance.

As described above, it will be understood that the technical configuration of the present invention described above can be implemented in other specific forms without changing the technical spirit or essential features of the present invention by those skilled in the art.

Therefore, the embodiments described above are to be understood as illustrative and non-limiting in all respects, and the scope of the present invention is indicated by the claims to be described later rather than the detailed description, and the meaning and scope of the claims and the All changes or modifications derived from the equivalent concept should be interpreted as being included in the scope of the present invention.

S10. The first step of mixing ginkgo leaf powder with distilled water, boiling water, and filtering to prepare a ginkgo leaf extract
S20. The second step of preparing a mixture by adding amino acids or N-acetyl amino acids and saccharides to the ginkgo leaf extract
S30. The third step of stirring and heating the prepared mixture
S40. The fourth step of adjusting the heated mixture to pH 3 to 6

Claims (5)

delete delete delete A first step of mixing 9 to 50% by weight of ginkgo leaf powder and 50 to 91% by weight of distilled water, bathing at 50 to 80°C for 2 to 5 hours, and filtering to prepare a ginkgo leaf extract;
A second step of preparing a mixture by mixing 97 to 99% by weight of the gingko leaf extract, 0.3 to 1% by weight of amino acids or N-acetyl amino acids, and 0.7 to 2% by weight of saccharides;
A third step of stirring and heating the prepared mixture;
Including; a fourth step of adjusting the heated mixture to pH 3 to 6;
The amino acid or N-acetyl amino acid,
At least any one of methionine, cysteine, cystine, N-acetyl cysteine, N-acetyl methionine, and N-acetyl proline,
The saccharide is,
At least one of fructose, glucose, sucrose, maltose, and xylose,
The pH adjustment,
It is carried out using at least one of citric acid, wood vinegar, and brown rice vinegar
Heating in the third step,
A 3-1 step of performing primary heating of the prepared mixture at 80 to 110°C for 2 hours;
3-2 step of performing the secondary heating while stirring the first heated mixture at 40 to 55° C. for 10 hours; S-1 step of mixing 9 to 50% by weight of ginkgo leaf powder and 50 to 91% by weight of distilled water, bathing at 50 to 80°C for 2 to 5 hours, and then filtering to prepare a ginkgo leaf extract;
The S-2 step of preparing a mixture by mixing 97 to 99% by weight of the gingko leaf extract, 0.3 to 1% by weight of amino acids or N-acetyl amino acids, and 0.7 to 2% by weight of saccharides;
S-3 step of stirring and heating the prepared mixture;
Step S-4 of adjusting the heated mixture to pH 3 to 6;
The pH-adjusted mixture 70 to 91% by weight, vegetable extract 3 to 10% by weight, vegetable oil 3 to 10% by weight, the step S-5 of mixing 3 to 10% by weight of plant extract; including,
The amino acid or N-acetyl amino acid,
At least any one of methionine, cysteine, cystine, N-acetyl cysteine, N-acetyl methionine, and N-acetyl proline,
The saccharide is,
At least one of fructose, glucose, sucrose, maltose, and xylose,
The pH adjustment,
It is carried out using at least one of citric acid, wood vinegar, and brown rice vinegar,
Heating in the S-3 step,
S-3-1 step of performing the first heating of the prepared mixture at 80 to 110 ℃ 2 hours;
Including; the first step S-3-2 of stirring the first heated mixture for 10 hours at 40 to 55 ℃ proceeds the secondary heating
The vegetable extract is at least any one of pork potato extract, garlic extract, cheongyang pepper extract, onion extract,
The vegetable oil is at least one of clovebird oil, lavender oil, rosemary oil, thyme white oil, cotton seed oil, lemon eucalyptus oil, peppermint oil, marjoram oil, canola oil, oregano oil, cinnamon bark oil,
The plant extract is a method for preparing an insecticide composition using ginkgo leaves, characterized in that at least one of nicotine extract, gosam extract, pyrethrum extract, and neem extract

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