KR20140040896A - Insecticide or compost composition comprising the leaves of ginkgo biloba by water aging - Google Patents

Abstract

The present invention relates to an insecticide or a compost composition comprising an aged ginkgo leaf and water. The composition comprising an aged ginkgo leaf and water has not only a superior efficacy of controlling various kinds of pest damaging crops, but also an effect of increasing a plant growth, and therefore, an eco-friendly insecticide composition unlike an existing synthetic insecticide composition, which causes such an environmental pollution, a harmful effect on a human body, a crop tolerance, and an ecosystem disturbance, can be manufactured; and a farm income can be raised by an effect of an increase in a crop growth. [Reference numerals] (AA) Step of manufacturing ginkgo leaves into power with a particle size of 100-300 쨉m; (BB) Step of mixing 100-500 parts by weight of water to 100 parts by weight of ginkgo leaves; (CC) Step of aging the ginkgo powder and water mixture for 20 minutes to 5 hours at 25-50 °C

Description

[0001] The present invention relates to a pesticide or a fertilizer composition containing ginkgo biloba aged water. [0002] Ginkgo biloba by water aging [

The present invention relates to a pesticide or a fertilizer composition containing ginkgo biloba aged material. More specifically, the present invention relates to a pesticide or fertilizer composition containing the ginkgo biloba water aging product prepared by adding water to the ginkgo biloba powder having a particle size of 100 ~ 300μm and aged at 25 ~ 50 ℃.

In modern agriculture, almost everyone uses synthetic pesticides for pest control. However, due to the excessive use of chemical pesticides for a long time, ecosystem disturbance such as soil microbial and natural enemy degradation, water pollution, and environmental problems such as soil and pesticide residues of agricultural products have emerged as a big social issue. There is an urgent need for natural pesticides or pesticides which are low toxic, non-toxic, and non-toxic for realization. In addition, chemical pesticides that have been used for decades in the past have become resistant to pests, and as the time passes, the use of larger amounts of pesticides or the use of more deadly, But also causes severe social problems, which are a fatal hazard to the human body that directly ingests crops. As a result, studies on biological pesticides using natural pesticides to replace chemical pesticides have been actively conducted. Well-known plant-derived insecticides include pyrethroids, a component of the bug kingdom , and rotenones, isolated from Lonchcarpus nicou , and Melia azedarach var. (J. Agric. Food Chem., 2003, 51, 369-374) have also been popularized as insecticides, as are limonoid compounds isolated from the genus Japonica MAKINO, subtripinnata,

On the other hand, these insecticide components are present in a trace amount in a natural state, but their contents are often increased when the plant extract or plant is aged. The aging process is a method of inducing the structural change of the active ingredient by infiltrating the aged material into water or alcohol or making the extraction of the active ingredient smooth. As compared with the fermentation requiring the microorganism and the medium, aging is mainly performed at a low temperature (4 ~ 50 ° C) of water as a solvent. For example, a plant called Baekguyong contains a small amount of pulsatilla saponin D, which is an anti-cancer component. However, when the Baekguyong is matured, the hyderaginin glycoside contained in Baekguyong It has been confirmed that the content of the full-tattilasapotin D is increased by 2 to 3 times as the flour is converted into full-tilasapotin D (US Patent No. 7682638).

Ginkgo biloba (leaves of Ginkgo biloba ) contains 20 kinds of flavonoids and 5 kinds of terpenactones. Among them, triterpenelactones are known to have a controlling effect against the brown planthopper (ACS Symposium Series, Chapter 7, 658, 90-105), and ginkgolic acids have various insecticidal effects And the insecticidal effect against the spotted mite (J. korean Soc. Appl. Biol. Chem., 2005, 48, 150 ~ 154) have been studied. However, since most of the functional active ingredients contained in the ginkgo leaf are not easily synthesized due to their complicated structure, and their content is very small, a large amount of raw ginkgo leaf is required to ensure this. In addition, in order to concentrate or separate these components, a multistage physicochemical treatment is required. Therefore, it is not economical to directly separate these components with an insecticide.

On the other hand, in the process of ripening ginkgo, a tar-like substance having affinity is generated. The present inventors confirmed that when the ginkgo leaf powder is treated with water at 25 to 50 ° C, a large amount of the tar substance is produced , Water plays an important role in the formation of the tar-like materials, and when the aged material of the ginkgo biloba powder is used as a plant controlling agent, the controlling action against the plant and the insecticidal action against the insect pests are improved, The present invention can be completed. Also, it was confirmed that when the aged material of the ginkgo leaf is mixed with the fertilizer, it can be used as an environmentally friendly fertilizer composition having an insecticidal effect.

Although the technology relating to insecticides derived from Ginkgo biloba is disclosed in Korean Patent No. 415107 and Korean Patent Publication No. 2004-0035218, the insecticide derived from Ginkgo biloba disclosed in the prior art is a ginkgo leaf prepared by boiling Ginkgo biloba powder in hot water Although the technical features of the present invention are different from those of the present invention, Korean Patent Publication No. 2012-0001454 also discloses a composition for insect pest control using ginkgo leaf powder. In the above-mentioned literature, a gluing agent is added to ginkgo leaf powder It can be said that the technical features of the present invention are different from those of the present invention for improving the fixing power of the ginkgo leaf powder by using only the aging process for using the composition as a composition for insect repelling.

Korean Patent Laid-Open Publication No. 2007-0059511 discloses a method for producing a garden fertilizer containing a ginkgo leaf ingredient extracted with an acid and an alkali solution. However, this is different from the present invention in technical characteristics, No previous literature has been found on the fertilizer prepared using the composition prepared by aging the gingko leaf powder by the method of

USP 7682638 (Use of hederagenin 3-O- alpha -L-rhamnopyranosyl (1-2) - [beta -D-glucopyranosyl (1-4)] -? - L-arabinopyranoside or an extract from pulsatillae radix containing the same as a therapeutic agent for solid tumors, March 23, 2010. Registration) Korean Registered Patent No. 415107 (Ginkgo biloba extract, preparation method and use thereof, published on Jan. 13, 2004) Korean Patent Publication No. 2004-0035218 (provision of pesticide composition, published Apr. 24, 2004) Korea Patent Publication No. 2012-0001454 (composition for insect pest control, published Jan. 14, 2012) Korean Patent Publication No. 2007-0059511 (Production method of a garden fertilizer containing a substance extracted with an acid and an alkali solution from a ginkgo leaf, disclosed on Jun. 12, 2007)

Carpinella, M.C. et al., Antifeedant and insecticide properties of a limonoid from Meliaceae [Meliaceae] with potential use for pest management, J. Agric. Food Chem., 2003, 51, 369-374. Ahn, Y.J. et al, Phytochemicals for Pest Control. ACS Symposium Series, Chapter 7, 658, 90-105. Insecticidal and Repellent Effects of Ginkgo Biloba Extract on Lepidoptera, Lepidoptera, Lepidoptera, Pseudomonas spp. Appl. Biol. Chem., 2005, 48, 150-154.

It is an object of the present invention to provide a pesticide or fertilizer composition containing ginkgo biloba aged material. More specifically, it is an object of the present invention to provide an insecticide or a fertilizer composition containing ginkgo biloba aged product prepared by adding water to a ginkgo leaf powder having a particle size of 100 to 300 mu m and aging at 25 to 50 < 0 > C.

The present invention relates to an insecticide, fungicide or antiviral composition containing ginkgo biloba aged material.

Preferably, the ginkgo biloba aged product is prepared by adding water to a gingko leaf powder having a particle size of 100 to 300 mu m and aging at 25 to 50 < 0 > C. More preferably, the ginkgo biloba aged product may be prepared by adding 100 to 500 parts by weight of water to 100 parts by weight of ginkgo leaf powder having a particle size of 100 to 300 mu m and aging at 25 to 50 < 0 > C.

The ginkgo leaf water-aged product may be a powdery composition obtained by drying a mixture of ginkgo leaf powder and water at 25 to 50 ° C while keeping the reaction product containing water and ginkgo leaf powder.

The ginkgo biloba aged material may be a liquid phase obtained by removing the powder from the reactant, or a composition in the form of a dried and dried state.

The composition containing the ginkgo biloba aged material may further contain a mull bean fruit powder having an insecticidal effect.

Meanwhile, the composition containing the ginkgo biloba extract of the present invention may further contain a plant nutrient, an agricultural chemical, or a mixture of two or more thereof.

The composition containing the ginkgo biloba aged product is a composition containing a ginkgo biloba aphid, aphid aphid, black aphid, peach aphid, cotton aphid, spotted mite, greenhouse powder, thrips worm, Moths moths, moths moths, moths moths moths, pear trees, moth pears, pear moths worms, brown moths, rice moths moths, rice moths moths, rice moths moths, rice moths moths, rice moths moths, rice weevils, At least one member selected from the group consisting of moths, Chinese cabbage, Chinese tobacco mosquito nets, moths, moth larvae, turtles, charcoal black moths, larvae, vertebrates, root mites, rootstock nematodes, soil insect pests and soil nematodes Pests, or fusarium oxysporums oxysporum , causal agent of wilt), Cylindrocarpon destructans , root rot bacteria), Botrytis cinerea, gray mold pathogen), Colle Saturday kyum geuloh Eos tree forest death (Colletotrichum gloeosporioides , anthrax causal organisms), Sclerotium cepivorum ( black causative organism causative organism), Sclerotinia sclerotarium ( Sclerotinia sclerotiorum , causative organism), Penicillium hirsutum , causative bacteria of blue mold), Pectobacterium carotovorum , a causative agent), Rhizoctonia solani , causative agent), Pythium ultimum , causative agent), Pythium one or more phytotoxic strains selected from the group consisting of Corynebacterium tuberculosis , Deboraryanum , Staphylococcus aureus and Rhizopus sp., or Tobacco mosaic virus, Cucumber mosaic virus, And potato virus Y. The plant virus may be used as a control agent against at least one plant virus.

In another aspect, the present invention can provide a fertilizer composition comprising the composition containing the ginkgo biloba aged material.

The present invention, on the other hand,

Preparing gingko leaf as a powder having a particle size of 100 to 300 mu m;

Mixing 100 to 500 parts by weight of water with 100 parts by weight of the ginkgo leaf powder; And

Aging the mixture of ginkgo leaf powder and water at 25 to 50 캜;

The present invention also provides a method for producing ginkgo biloba aged product.

Hereinafter, the present invention will be described in more detail.

The ginkgo biloba aged material may be prepared by pulverizing dried ginkgo leaves in a particle size of 100 to 300 mu m and aging with water.

The ginkgo leaf water-aged product may be a mixture of ginkgo leaf powder and water, which is aged at 25 to 50 ° C to use the reactant as it is. In addition, the ginkgo biloba aged product may be a powdery composition obtained by drying a mixture of ginkgo leaf powder and water at 25 to 50 < 0 > C with the reaction product containing water and ginkgo leaf powder. On the other hand, the ginkgo biloba aged material may be a liquid (filtrate) obtained by removing powder from the reactant, or a composition in the form of a dried form of the dried liquid. As the drying method, a conventional drying method, preferably freeze drying, spray drying, vacuum drying, cold air drying and the like may be used.

The ginkgo biloba water aging can be prepared using 100-300 μm particle size ginkgo leaf powder, preferably 100-200 μm, more preferably 100-150 μm. If the size of the ginkgo leaf powder is more than 300 袖 m, the gingko leaf water-aging product is insoluble, fungicidal, antiviral, or sticking force deteriorates and is not suitable for pesticide, fungicide or antiviral composition. On the contrary, even if the size of the ginkgo leaf powder is less than 100 탆, insolubility, fungicidal activity, antiviral property or binding force is poor. Particularly when the ginkgo leaf powder size is less than 70 탆, when the ginkgo biloba aged product is powdered and sprayed, The adhesion to the plant is significantly lowered, and the insecticide, fungicidal or antiviral properties are also lowered.

The ginkgo biloba aged product may be prepared by adding 100 to 500 parts by weight of water to 100 parts by weight of the ginkgo leaf powder having a particle size of 100 to 300 mu m, and preferably 200 to 300 parts by weight of water. At this time, if the weight of water is less than 100 parts by weight, the amount of water is so small that aging is not sufficient. If the weight of water exceeds 500 parts by weight, the ginkgo leaf powder may not be aged well and the insecticidal effect may not be high. In particular, when the ginkgo biloba aged material is prepared into powder, the drying time becomes long and the cost of drying Which is undesirable.

The ginkgo biloba aged material can be aged at 25 to 50 ° C, preferably 30 to 40 ° C. If the aging temperature is less than 25 ° C, aging is not performed well. If the aging temperature is more than 50 ° C, the biochemical reaction including the enzyme reaction during aging is not smooth and the aging effect is not shown well.

The ginkgo biloba aged product may be prepared by aging for 20 minutes to 24 hours, preferably 20 minutes to 5 hours, more preferably 20 to 60 minutes, and most preferably 30 to 40 minutes. At this time, when the aging time is less than 20 minutes, aging is not performed well. On the other hand, even if the aging time exceeds 5 hours, the aging does not proceed further. However, the aging effect is maintained until 24 hours. If the aging time exceeds 24 hours, the aging reaction may not be performed due to an unnecessary enzymatic reaction.

After the aging reaction, the ginkgo biloba aged material may be heat-treated to remove the biological reaction in the aged material. The heat treatment may be performed at 70 to 120 ° C for 5 to 15 minutes, preferably at 90 to 100 ° C for 5 to 10 minutes.

In addition, the ginkgo biloba aged product may further contain a natural product powder having an insecticidal effect, a plant nutrient, an agricultural chemical, or a mixture of two or more thereof.

Examples of the natural powder include dry powder such as marbled bean, pestilent, garlic, rhubarb, mugwort gum, gosang, and mackerel, or extract powder thereof. Among them, especially the fruit of the mulberry tree has excellent insecticidal effect, and is known to contain a large amount of theophylline component that acts on the insect pests. The composition of the present invention may also contain nem oil extracted and purified from the mulberry fruit, which has an insect control effect (an insect's decline in an insect's pests, growth retardation, reproductive dysfunction, repellent effect, . The particle size of the powder of the mull bean fruit is not particularly limited, but is preferably 100 to 300 탆.

As a plant nutrient that can be added to the ginkgo biloba aged material of the present invention, a conventionally used plant nutrient supply fertilizer can be used. As the above-mentioned fertilizer, organic fertilizer, compound fertilizer, nitrogen fertilizer, phosphoric acid fertilizer, calcium fertilizer, lime fertilizer, lactic acid fertilizer, sulfuric acid fertilizer, magnesium fertilizer, trace element fertilizer, organic fertilizer and manure fertilizer can be used. The composition comprising the ginkgo biloba aged material and the plant nutrient of the present invention can be used as a plant nutrient which is excellent in the control effect against various pests and is useful for plant growth.

The pesticide which can be added to the ginkgo biloba aged material of the present invention may include pesticides conventionally used. Preferably, the insecticide is selected from the group consisting of carbamates, organic phosphates, organic chlorine insecticides, phenylpyrazole, pyrethroids, neonitinoids, spinosin, avermectin, milbemycin, larval hormone analogs, Alkyl halides, organotin compounds, nereistoxin analogues, benzoylureas, diacylhydrazines, insecticides of the class of the METI acaricides, and chlorpicillin, pimetrozine, floninamide, But are not limited to, but are not limited to, cyclophosphamide, cyclophosphamide, cyclophosphamide, cyclophosphamide, cyclophosphamide, cyclophosphamide, Pesticides such as derivatives thereof can be used.

In addition, the insecticide, fungicide or antiviral composition containing the ginkgo biloba aged product may be mixed with other commonly used active ingredients or excipients such as excipients, or may be further added with pigments, surfactants, preservatives, etc. .

The insecticide, fungicide or antiviral composition composition containing the ginkgo biloba fermented product may be packaged and commercialized by a conventional method such as pulverization, pelletization, granulation, and tableting. In addition, the insecticide, fungicide or antiviral composition containing the ripened ginkgo biloba water may be used through various methods depending on its form, application purpose, and application field, such as a method of dissolving or diluting in water and spraying. The dose may be appropriately used depending on the purpose of application, the type of application, the area of application, the application target, and the like. However, the pesticide, fungicide or antiviral composition containing the ginkgo biloba fermented product of the present invention does not particularly limit the amount or application method.

In addition, the present invention can provide a foliar spraying agent, a foliar spraying agent, a plant nutrient, a seed coating agent, a soil improving agent, and a composting homework agent containing the insecticide, fungicide or antiviral composition.

In another aspect, the present invention can provide a fertilizer composition comprising a composition containing gingko leaf water aged. Since the fertilizer composition contains ginkgo biloba aged material, it is excellent in insecticidal, fungicidal or antiviral effect, and it is possible to provide nutritional supply, insecticide, fungus, and antifungal agent without spraying a separate insecticide, fungicide or antiviral ingredient The virus effect can be exhibited.

It is preferred that the fertilizer composition is usually mixed with the soil 3 to 4 days before planting. At this time, the fertilizer composition may be used in an amount of 0.5 to 2 kg per 100 square meters.

In addition, the fertilizer composition is preferably mixed with a conventionally prepared fertilizer and a composition containing the ginkgo biloba aged material of the present invention. Also, composts conventionally produced as the above fertilizer may be used. The compost can be produced by fermentation in one to three stages. At this time, as a material for manufacturing the compost, there are used various kinds of materials such as feces, waste paper, sewage disposal sludge, food processing materials, food waste, mushroom waste medium, mugwort, bean sprouts, green tea sludge, bagasse, rice straw, A mixture of at least one selected from the group consisting of house, bean stem, cornstalk, hay, sawdust, bark, leaves, animal bone, hull, crab shell, egg shell, animal manure (cattle, pigs, Water may be added to make the water content 50% or more, followed by natural fermentation at 20 to 60 ° C for 3 to 10 days. In this case, various fermentation strains may be mixed to produce a good quality compost. Preferably, one or more strains selected from the group consisting of lactic acid bacteria, yeast, Bacillus strains, and Aspergillus strains may be used. The compost produced by the above method may be used as it is or may be dried to a moisture content of 10 to 20% or dried or powdered or pelletized for long-term preservation.

In addition, the composition containing the ginkgo biloba of the present invention may be contained in the total fertilizer composition in an amount of 30 to 70% by weight.

The present invention relates to a pesticide or a fertilizer composition containing ginkgo biloba aged material. The composition containing the ginkgo biloba aging material has an excellent effect of controlling various insect pests damaging to crops, but also has an effect of increasing plant growth, and is effective in improving the growth of plants with environmental pollution and human harmfulness, crop tolerance and ecosystem disturbance An environmentally friendly pesticide composition different from the conventional synthetic pesticide composition can be produced, and the increase in crop propagation can lead to an increase in income of the farm household.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart showing the step of producing the ginkgo biloba of the present invention. Fig.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

< Example  1. Preparation of ginkgo leaf powder>

Example  1-1. Ginkgo leaf powder with a particle size of 100 μm

The gingko leaf was dried to a humidity of 7% or less and ground with a jet mill (Zet-Mil) to obtain a ginkgo leaf powder having a particle size of 100 μm.

Example  1-2. Ginkgo leaf powder with a particle size of 150 μm

Ginkgo leaf powder having a particle size of 150 mu m was prepared in the same manner as in Example 1-1.

Example  1-3. Ginkgo leaf powder with a particle size of 300 μm

Ginkgo leaf powder having a particle size of 300 mu m was prepared in the same manner as in Example 1-1.

< Example  2. Ginkgo leaf water Aged material  Manufacturing-I>

The ginkgo leaf powder prepared in Example 1 was mixed with water and reacted (aged) at 25 to 50 ° C for 20 minutes to 5 hours under the conditions shown in Table 1 below. Thereafter, the filtrate obtained by filtering the reactant was used as a ginkgo leaf water-aged product. Further, water was added to the filtrate, or the filtrate was concentrated to uniformize the volume of each sample to 300 ml.

Condition Example
1-1 of
Ginkgo leaf powder Example
1-2
Ginkgo leaf powder Example
1-3 of
Ginkgo leaf powder
water
ferment
Temperature
ferment
time Example 2-1 100g - - 100g 35 ℃ 30 minutes Example 2-2 100g - - 300g 35 ℃ 30 minutes Example 2-3 100g - - 500g 35 ℃ 30 minutes Examples 2-4 100g - - 200g 25 ℃ 30 minutes Example 2-5 100g - - 200g 50 ℃ 30 minutes Examples 2-6 100g - - 200g 35 ℃ 20 minutes Examples 2-7 100g - - 200g 35 ℃ 5 hours Examples 2-8 - 100g - 100g 35 ℃ 30 minutes Examples 2-9 - 100g - 300g 35 ℃ 30 minutes Examples 2-10 - 100g - 500g 35 ℃ 30 minutes Examples 2-11 - 100g - 200g 25 ℃ 30 minutes Examples 2-12 - 100g - 200g 50 ℃ 30 minutes Examples 2-13 - 100g - 200g 35 ℃ 20 minutes Examples 2-14 - 100g - 200g 35 ℃ 5 hours Examples 2-15 - - 100g 100g 35 ℃ 30 minutes Examples 2-16 - - 100g 300g 35 ℃ 30 minutes Examples 2-17 - - 100g 500g 35 ℃ 30 minutes Examples 2-18 - - 100g 200g 25 ℃ 30 minutes Example 2-19 - - 100g 200g 50 ℃ 30 minutes Examples 2-20 - - 100g 200g 35 ℃ 20 minutes Examples 2-21 - - 100g 200g 35 ℃ 5 hours

< Example  3. Ginkgo leaves Aging  Produce- II >

The ginkgo leaf powder prepared in Example 1 was mixed with water and reacted (aged) at 25 to 50 ° C for 20 minutes to 5 hours under the conditions shown in Table 2 below. The reaction mixture was reacted in a water bath at 100 ° C for 10 minutes And the biochemical reaction in the ginkgo leaf powder was stopped. Thereafter, the reaction product was immediately added to the dryer without filtration and dried at a temperature of 40 ° C or less under reduced pressure with stirring for 12 hours. The powder was used as a ginkgo biloba aged material.

Condition Example
1-1 of
Ginkgo leaf powder Example
1-2
Ginkgo leaf powder Example
1-3 of
Ginkgo leaf powder
water
ferment
Temperature
ferment
time Example 3-1 100g - - 100g 35 ℃ 30 minutes Example 3-2 100g - - 300g 35 ℃ 30 minutes Example 3-3 100g - - 500g 35 ℃ 30 minutes Example 3-4 100g - - 200g 25 ℃ 30 minutes Example 3-5 100g - - 200g 50 ℃ 30 minutes Examples 3-6 100g - - 200g 35 ℃ 20 minutes Examples 3-7 100g - - 200g 35 ℃ 5 hours Examples 3-8 - 100g - 100g 35 ℃ 30 minutes Examples 3-9 - 100g - 300g 35 ℃ 30 minutes Examples 3-10 - 100g - 500g 35 ℃ 30 minutes Examples 3-11 - 100g - 200g 25 ℃ 30 minutes Examples 3-12 - 100g - 200g 50 ℃ 30 minutes Examples 3-13 - 100g - 200g 35 ℃ 20 minutes Examples 3-14 - 100g - 200g 35 ℃ 5 hours Examples 3-15 - - 100g 100g 35 ℃ 30 minutes Examples 3-16 - - 100g 300g 35 ℃ 30 minutes Examples 3-17 - - 100g 500g 35 ℃ 30 minutes Examples 3-18 - - 100g 200g 25 ℃ 30 minutes Examples 3-19 - - 100g 200g 50 ℃ 30 minutes Examples 3-20 - - 100g 200g 35 ℃ 20 minutes Examples 3-21 - - 100g 200g 35 ℃ 5 hours

< Example  4. Ginkgo leaves Aged water Farbell  Preparation of mixture of fruit powder>

Example  4-1. Ginkgo water Aged water Farbell  4: 1 (w: w) blend of the fruit powder

80 g of the ginkgo biloba aged material of Example 3-9 and 20 g of dried beaded fruit powder having a particle size of 150 mu m were mixed.

Example  4-2. Ginkgo water Aged water Farbell  1: 1 (w: w) blend of the fruit powder

50 g of the ginkgo biloba aged material of Example 3-9 and 50 g of dried beads with a particle size of 150 mu m were mixed.

< Comparative Example  1. Preparation of comparative ginkgo leaf powder>

Comparative Example  1-1. Ginkgo leaf powder having a particle size of 70 μm

Ginkgo leaf powder having a particle size of 70 mu m was prepared in the same manner as in Example 1-1.

Comparative Example  1-2. Ginkgo leaf powder with a particle size of 400 μm

Ginkgo leaf powder having a particle size of 400 mu m was prepared in the same manner as in Example 1-1.

< Comparative Example  2. Comparison of Ginkgo leaves Aging  Manufacturing-I>

The ginkgo leaf powder prepared in Example 1 and Comparative Example 1 was mixed with water and aged under the conditions shown in Table 3. The reaction was then allowed to react for 10 minutes in a water bath at 100 ° C to stop the biochemical reaction in the ginkgo leaf powder . Thereafter, the filtrate obtained by filtering the gingko leaf powder from the reaction product was used as a ginkgo biloba aged material. Further, water was added to the filtrate, or the filtrate was concentrated to uniformize the volume of each sample to 300 ml.

Condition Example
1-1 of
Ginkgo leaf
powder Example
1-2
Ginkgo leaf
powder Example
1-3 of
Ginkgo leaf
powder Comparative Example
1-1 of
Ginkgo leaf
powder Comparative Example
1-2
Ginkgo leaf
powder
water
ferment
Temperature
ferment
time Comparative Example 2-1 100g - - - - 600g 35 ℃ 30 minutes Comparative Example 2-2 100g - - - - 200g 20 ℃ 30 minutes Comparative Example 2-3 100g - - - - 200g 60 ° C 30 minutes Comparative Example 2-4 100g - - - - 200g 35 ℃ 10 minutes Comparative Example 2-5 100g - - - - 200g 35 ℃ 24 hours Comparative Example 2-6 - 100g - - - 600g 35 ℃ 30 minutes Comparative Example 2-7 - 100g - - - 200g 20 ℃ 30 minutes Comparative Example 2-8 - 100g - - - 200g 60 ° C 30 minutes Comparative Example 2-9 - 100g - - - 200g 35 ℃ 10 minutes Comparative Example 2-10 - 100g - - - 200g 35 ℃ 24 hours Comparative Example 2-11 - - 100g - - 600g 35 ℃ 30 minutes Comparative Examples 2-12 - - 100g - - 200g 20 ℃ 30 minutes Comparative Example 2-13 - - 100g - - 200g 60 ° C 30 minutes Comparative Example 2-14 - - 100g - - 200g 35 ℃ 10 minutes Comparative Example 2-15 - - 100g - - 200g 35 ℃ 24 hours Comparative Example 2-16 - - - 100g - 600g 60 ° C 30 minutes Comparative Example 2-17 - - - 100g - 200g 35 ℃ 30 minutes Comparative Example 2-18 - - - 100g - 200g 35 ℃ 30 minutes Comparative Example 2-19 - - - 100g - 200g 35 ℃ 10 minutes Comparative Example 2-20 - - - 100g - 200g 35 ℃ 24 hours Comparative Example 2-21 - - - 100g - 200g 35 ℃ 30 minutes Comparative Example 2-22 - - - - 100g 600g 60 ° C 30 minutes Comparative Example 2-23 - - - - 100g 200g 35 ℃ 30 minutes Comparative Example 2-24 - - - - 100g 200g 35 ℃ 30 minutes Comparative Example 2-25 - - - - 100g 200g 35 ℃ 10 minutes Comparative Example 2-26 - - - - 100g 200g 35 ℃ 24 hours Comparative Example 2-27 - - - - 100g 200g 35 ℃ 30 minutes Comparative Example 2-28 - 100g - - - 200g 100 ℃ 30 minutes

< Comparative Example  3. Comparison of Ginkgo leaves Aging  Produce- II >

The ginkgo leaf powder prepared in Example 1 and Comparative Example 1 was mixed with water and aged under the conditions shown in Table 4. The reaction was then allowed to react for 10 minutes in a water bath at 100 ° C to stop the biochemical reaction in the ginkgo leaf powder . Thereafter, the reaction product was immediately added to the dryer without filtration, and dried at a temperature of 40 DEG C or lower for 12 hours under reduced pressure with stirring, thereby obtaining a powder The ginkgo leaves were used as aged water.

Condition Example
1-1 of
Ginkgo leaf
powder Example
1-2
Ginkgo leaf
powder Example
1-3 of
Ginkgo leaf
powder Comparative Example
1-1 of
Ginkgo leaf
powder Comparative Example
1-2
Ginkgo leaf
powder
water
ferment
Temperature
ferment
time Comparative Example 3-1 100g - - - - 550g 35 ℃ 30 minutes Comparative Example 3-2 100g - - - - 200g 20 ℃ 30 minutes Comparative Example 3-3 100g - - - - 200g 55 ° C 30 minutes Comparative Example 3-4 100g - - - - 200g 35 ℃ 10 minutes Comparative Example 3-5 100g - - - - 200g 35 ℃ 24 hours Comparative Example 3-6 - 100g - - - 550g 35 ℃ 30 minutes Comparative Example 3-7 - 100g - - - 200g 20 ℃ 30 minutes Comparative Example 3-8 - 100g - - - 200g 55 ° C 30 minutes Comparative Example 3-9 - 100g - - - 200g 35 ℃ 10 minutes Comparative Example 3-10 - 100g - - - 200g 35 ℃ 24 hours Comparative Example 3-11 - - 100g - - 550g 35 ℃ 30 minutes Comparative Examples 3-12 - - 100g - - 200g 20 ℃ 30 minutes Comparative Example 3-13 - - 100g - - 200g 55 ° C 30 minutes Comparative Example 3-14 - - 100g - - 200g 35 ℃ 10 minutes Comparative Example 3-15 - - 100g - - 200g 35 ℃ 24 hours Comparative Example 3-16 - - - 100g - 550g 55 ° C 30 minutes Comparative Example 3-17 - - - 100g - 200g 35 ℃ 30 minutes Comparative Example 3-18 - - - 100g - 200g 35 ℃ 30 minutes Comparative Example 3-19 - - - 100g - 200g 35 ℃ 10 minutes Comparative Example 3-20 - - - 100g - 200g 35 ℃ 24 hours Comparative Example 3-21 - - - 100g - 200g 35 ℃ 30 minutes Comparative Example 3-22 - - - - 100g 550g 55 ° C 30 minutes Comparative Example 3-23 - - - - 100g 200g 35 ℃ 30 minutes Comparative Example 3-24 - - - - 100g 200g 35 ℃ 30 minutes Comparative Example 3-25 - - - - 100g 200g 35 ℃ 10 minutes Comparative Example 3-26 - - - - 100g 200g 35 ℃ 24 hours Comparative Example 3-27 - - - - 100g 200g 35 ℃ 30 minutes Comparative Example 3-28 - 100g - - - 200g 100 ℃ 30 minutes

< Comparative Example  4. Gingko leaf Non-aged  Manufacturing>

Comparative Example  4-1. Using ginkgo leaf powder with a particle size of 150 μm

To 100 g of the ginkgo leaf powder of Example 1-2, 200 g of water at 100 DEG C was added and reacted for 10 minutes (the aging step was omitted and only the biochemical reaction in the ginkgo leaf powder was stopped). Thereafter, the reactant was immediately added to the dryer without filtration, and dried at a temperature of 40 ° C or less under reduced pressure with stirring for 12 hours. The powder was used as non-aged ginkgo biloba.

Comparative Example  4-2. Using ginkgo leaf powder with a particle size of 300 μm

200 g of 100 캜 water was added to 100 g of the ginkgo leaf powder of Example 1-3 and reacted for 10 minutes (the aging step was omitted and only the biochemical reaction in the ginkgo leaf powder was stopped). Thereafter, the reactant was immediately added to the dryer without filtration, and dried at a temperature of 40 ° C or less under reduced pressure with stirring for 12 hours. The powder was used as non-aged ginkgo biloba.

Comparative Example  4-3. Ginkgo leaves prepared by using ginkgo leaf powder having a particle size of 400 탆 Non-aged  Produce

200 g of water at 100 占 폚 was added to 100 g of the ginkgo leaf powder of Comparative Example 1-2 and reacted for 10 minutes (the aging step was omitted and only the biochemical reaction in the ginkgo leaf powder was stopped). Thereafter, the reactant was immediately added to the dryer without filtration, and dried at a temperature of 40 ° C or less under reduced pressure with stirring for 12 hours. The powder was used as non-aged ginkgo biloba.

< Comparative Example  5. Ginkgo leaf Non-aged water and Farbell  Preparation of fruit mixture>

80 g of the gingko leaf non-aged material of Comparative Example 4-1 and 20 g of the bead powder having a particle size of 150 m were mixed.

< Comparative Example  6. Farbell  Preparation of fruit powder &gt;

100 g of the bead powder was ground to a particle size of 150 mu m.

< Experimental Example  1. Liquid Ginkgo leaf water Aging  Checking the effect of controlling the bean mite spotted mite>

The control effect of the ginkgo biloba fermented product of the present invention was confirmed by using a method known in the art (Lee, In-Hwa, Ji-Sung, Park Jong-Dae, J. Korean Soc. Appl. Biol. Chem., 48, 150). The experiment was carried out by inoculating 40 dogs per mugwort. The canola was cultivated in a plastic house at 25 ° C, and the mite was inoculated on the soybean leaves in the cultivation of the vinyl house. Thereafter, the spore mite leaves were placed in a Petri dish having a diameter of 105 cm, and then a sprayer containing liquid of Ginkgo biloba juice in the liquid state of Example 2 and Comparative Example 2 was placed on a stand, Cm, and sprayed three times so that the surface of the bean leaves was evenly wetted. The number of live spotted mite under magnifying glass was checked every 2 hours after spraying, and the results are shown in Table 5.

Condition Number of individuals of live spotted mite after watering of ginkgo leaf water (mari) Example 2-1 5 Example 2-2 4 Example 2-3 5 Examples 2-4 4 Example 2-5 3 Examples 2-6 5 Examples 2-7 4 Examples 2-8 0 Examples 2-9 0 Examples 2-10 3 Examples 2-11 One Examples 2-12 2 Examples 2-13 One Examples 2-14 0 Examples 2-15 0 Examples 2-16 3 Examples 2-17 4 Examples 2-18 3 Example 2-19 4 Examples 2-20 2 Examples 2-21 4 Comparative Example 2-1 32 Comparative Example 2-2 35 Comparative Example 2-3 33 Comparative Example 2-4 34 Comparative Example 2-5 25 Comparative Example 2-6 30 Comparative Example 2-7 32 Comparative Example 2-8 32 Comparative Example 2-9 28 Comparative Example 2-10 19 Comparative Example 2-11 33 Comparative Examples 2-12 34 Comparative Example 2-13 35 Comparative Example 2-14 32 Comparative Example 2-15 23 Comparative Example 2-16 29 Comparative Example 2-17 31 Comparative Example 2-18 32 Comparative Example 2-19 33 Comparative Example 2-20 16 Comparative Example 2-21 33 Comparative Example 2-22 35 Comparative Example 2-23 32 Comparative Example 2-24 33 Comparative Example 2-25 29 Comparative Example 2-26 23 Comparative Example 2-27 35 Comparative Example 2-28 30 Control group
(Untreated group)
40

From the results of Table 5, it was found that in the case of the soybean leaf treated with the ginkgo biloba extract of the present invention, the spotted mite was almost killed, whereas in the case of the soybean leaf treated with the ginkgo biloba of Comparative Example, the mite mite was mostly alive. In addition, although the aging time of the ginkgo leaf powder was 5 hours, the aging effect of the ginkgo leaf powder was excellent, whereas in the case of Comparative Examples 2-5, 2-10, 2-15, 2-20 and 2-26 in which the aging time of the ginkgo leaf powder was 24 hours , And it was confirmed that the effect was slightly lower than those that had been aged for 5 hours.

< Experimental Example  2. Ginkgo leaf water Aging  For plant leaf Fastening force  Check>

When the ginkgo biloba in powder form is directly sprayed on plants and used as a controlling agent, the effect of sticking the ginkgo biloba to plants for a long period of time enhances the control effect. That is, by increasing the fastening power of the ginkgo biloba, the active ingredients extracted from the gingko leaf are attached to the plant for a long time to enhance the control effect. Thus, in this experiment, the ginkgo biloba aged material of Example 3, the mixture of ginkgo biloba aged material and mulberry beverage powder of Example 4, the ginkgo leaf aged material of Comparative Example 3, and the ginkgo leaf non-aging material of Comparative Example 4 The binding power of the ginkgo leaf non-aged material of Example 5 and the powder mixed with the mulberry fruit powder and the mulberry fruit powder of Comparative Example 6 was confirmed.

First, the persimmon leaves were cut to a size of 5 × 5 cm and weighed. Then, the persimmon leaves were sprayed with water to soak the persimmon leaves in water, and the leaves of the persimmon leaves of Example 3, the leaves of the ginkgo leaf of Example 4 A mixture of aged water and mull bean fruit powder, a gingko leaf water aged material of Comparative Example 3, a ginkgo leaf non-aged material of Comparative Example 4, a powder of Comparative Example 5 in which ginkgo leaf non-aged material and mull bean fruit powder were mixed and a comparative example 6 mulberry fruit powder was sprinkled evenly 0.5 g each. Thereafter, the weight of the persimmon leaves was again determined, and the suspension was placed under an incandescent lamp at 25 ° C. and left for 5 hours. After 5 to 72 hours, the persimmon leaves were shaken to the ground paper to remove the powder sprayed on the persimmon leaves. Thereafter, the difference in weight of the powder attached to the persimmon leaves was confirmed and shown in Table 6 below. In this case, the numbers in Table 6 indicate the weight of the powder attached to the persimmon leaves, and the smaller the weight difference, the higher the fastening force.

Condition Ginkgo leaf attached to persimmon leaf Weight of water aged (g) Example 3-1 0.50 0.38 0.37 0.35 Example 3-2 0.50 0.36 0.35 0.32 Example 3-3 0.50 0.37 0.36 0.33 Example 3-4 0.50 0.36 0.35 0.34 Example 3-5 0.50 0.36 0.35 0.33 Examples 3-6 0.50 0.37 0.36 0.33 Examples 3-7 0.50 0.37 0.36 0.32 Examples 3-8 0.50 0.40 0.38 0.35 Examples 3-9 0.50 0.39 0.39 0.34 Examples 3-10 0.50 0.37 0.38 0.37 Examples 3-11 0.50 0.38 0.38 0.36 Examples 3-12 0.50 0.39 0.39 0.34 Examples 3-13 0.50 0.38 0.39 0.33 Examples 3-14 0.50 0.34 0.38 0.34 Examples 3-15 0.50 0.33 0.38 0.32 Examples 3-16 0.50 0.35 0.33 0.32 Examples 3-17 0.50 0.34 0.32 0.31 Examples 3-18 0.50 0.35 0.33 0.31 Examples 3-19 0.50 0.35 0.33 0.30 Examples 3-20 0.50 0.35 0.32 0.30 Examples 3-21 0.50 0.34 0.33 0.30 Example 4-1 0.50 0.35 0.39 0.33 Example 4-2 0.50 0.36 0.38 0.33 Comparative Example 3-1 0.50 0.15 0.08 0.01 Comparative Example 3-2 0.50 0.15 0.09 0.02 Comparative Example 3-3 0.50 0.14 0.09 0.01 Comparative Example 3-4 0.50 0.15 0.10 0.02 Comparative Example 3-5 0.50 0.15 0.09 0.03 Comparative Example 3-6 0.50 0.13 0.07 0.03 Comparative Example 3-7 0.50 0.14 0.06 0.02 Comparative Example 3-8 0.50 0.12 0.07 0.03 Comparative Example 3-9 0.50 0.15 0.08 0.02 Comparative Example 3-10 0.50 0.16 0.06 0.02 Comparative Example 3-11 0.50 0.13 0.03 0.01 Comparative Examples 3-12 0.50 0.13 0.03 0.01 Comparative Example 3-13 0.50 0.14 0.03 0.01 Comparative Example 3-14 0.50 0.14 0.02 0.01 Comparative Example 3-15 0.50 0.15 0.03 0.01 Comparative Example 3-16 0.50 0.01 0.00 0.00 Comparative Example 3-17 0.50 0.01 0.00 0.00 Comparative Example 3-18 0.50 0.01 0.00 0.00 Comparative Example 3-19 0.50 0.01 0.00 0.00 Comparative Example 3-20 0.50 0.01 0.00 0.00 Comparative Example 3-21 0.50 0.01 0.00 0.00 Comparative Example 3-22 0.50 0.12 0.04 0.01 Comparative Example 3-23 0.50 0.11 0.05 0.01 Comparative Example 3-24 0.50 0.12 0.03 0.01 Comparative Example 3-25 0.50 0.12 0.04 0.01 Comparative Example 3-26 0.50 0.11 0.03 0.01 Comparative Example 3-27 0.50 0.12 0.04 0.01 Comparative Example 3-28 0.50 0.10 0.02 0.01 Comparative Example 4-1 0.50 0.14 0.07 0.01 Comparative Example 4-2 0.50 0.18 0.06 0.01 Comparative Example 4-3 0.50 0.15 0.07 0.02 Comparative Example 5 0.50 0.15 0.05 0.01 Comparative Example 6 0.50 0.01 0.00 0.00

Referring to the results of Table 6 above, the binding strength of the ginkgo biloba aged material of Example 3 and the powder obtained by mixing the ginkgo biloba powder and the ginkgo biloba powder of Example 4 were as shown in Comparative Example 3, 4 ginkgoose non-aged material, the ginkgo leaf of Comparative Example 5, and the bead powder of Comparative Example 6, and the bead powder of Comparative Example 6, respectively.

It was also confirmed that the binding power of the ginkgo biloba powder of Example 4 and the powder of the mulberry beverage powder were kept the same as those of the ginkgo biloba aged material of Example 3. It was also found that the fixing powers of the powders of Examples 3 and 4 of the present invention were maintained to the same degree after 5 hours, but that the fixing strength of the powders of Comparative Examples 3 to 5 decreased with time. Also, in Comparative Example 3-19 having a particle size of 70 탆, the powder of Comparative Example 3-25 and the powder of Comparative Example 6 containing only the mull bean fruit powder were hardly fixed to the leaves and dispersed immediately after spraying.

In addition, the powder with a ginkgoage time of 5 hours of ginkgo leaf powder showed a good adhesion effect, whereas in the case of Comparative Examples 3-5, 3-10, 3-15 and 3-26 in which the ripening time of the ginkgo leaf powder was 24 hours, It was confirmed that the effect was slightly lower than that of the ripening. Therefore, it was judged that the aging time of the gingko leaf powder was 20 minutes to 5 hours in terms of the production cost and the production time.

In addition, the above tests were carried out at 20 ° C., 30 ° C. and 35 ° C. (temperature at the time of pest infestation in Korea) in addition to 25 ° C., but the change of fixation force with temperature was hardly observed.

< Experimental Example  3. Ginkgo leaves Aging  Checking the effect of apple tree leaf against spotted mite>

On August 25, 2010, 50 apple leaves (group 1) of low branches of apple trees were taken and 20 spots mites per leaf were inoculated on the bottom part of the leaves (total 1000) Were inoculated with the same spotted mite.

10 days later, at 10:00 am, water was sprinkled on the lower part of the leaf spotted leaf to wet the leaf evenly, and then the aged product of the ginkgo leaf of Example 3, the powder of the ginkgo leaf matured product of Example 4 and the powder , Ginkgo biloba aged material of Comparative Example 3, non-aged ginkgo leaf of Comparative Example 4, powder of the ginkgo leaf non-aging material of Comparative Example 5 and the powder of the mull bean fruit powder, and the mull bean fruit powder of Comparative Example 6, And sprayed with 10 g each. On the next day at 10 o'clock, 10 g of each of the powders was sprayed under the same conditions. After 72 hours of spraying, the number of the spotted mites remaining on the apple tree leaves under a magnifying glass after each leaf was examined and is shown in Table 7 below. On the other hand, no sample was treated in the control group.

Condition Number of individuals of the spotted mite remaining on apple tree leaves (horses) Example 3-1 45 Example 3-2 65 Example 3-3 74 Example 3-4 85 Example 3-5 63 Examples 3-6 54 Examples 3-7 65 Examples 3-8 27 Examples 3-9 23 Examples 3-10 35 Examples 3-11 20 Examples 3-12 22 Examples 3-13 35 Examples 3-14 42 Examples 3-15 31 Examples 3-16 75 Examples 3-17 64 Examples 3-18 55 Examples 3-19 86 Examples 3-20 67 Examples 3-21 64 Example 4-1 0 Example 4-2 One Comparative Example 3-1 556 Comparative Example 3-2 554 Comparative Example 3-3 535 Comparative Example 3-4 645 Comparative Example 3-5 267 Comparative Example 3-6 523 Comparative Example 3-7 523 Comparative Example 3-8 545 Comparative Example 3-9 563 Comparative Example 3-10 223 Comparative Example 3-11 565 Comparative Examples 3-12 587 Comparative Example 3-13 456 Comparative Example 3-14 589 Comparative Example 3-15 267 Comparative Example 3-16 856 Comparative Example 3-17 857 Comparative Example 3-18 853 Comparative Example 3-19 863 Comparative Example 3-20 853 Comparative Example 3-21 823 Comparative Example 3-22 538 Comparative Example 3-23 555 Comparative Example 3-24 546 Comparative Example 3-25 555 Comparative Example 3-26 221 Comparative Example 3-27 689 Comparative Example 3-28 753 Comparative Example 4-1 634 Comparative Example 4-2 656 Comparative Example 4-3 654 Comparative Example 5 556 Comparative Example 6 887 Control group 961

From the results of Table 7, it can be seen that the ginkgo biloba aged material of the present invention exhibits a high insecticidal power. Particularly, the powder obtained by mixing ginkgo biloba and mulberry fruit powder has the highest insecticidal rate.

< Experimental Example  4. Ginkgo leaves Aging Mustard radish  Checking the effectiveness of anti-

If the mustard seeds are grown in a field, the risk of the insect pests is very large. In this experiment, we observed the effect of aged ginkgo biloba on the pests and diseases of mustard radish.

30 pieces of potted plants grown with four leaves of mustard seeds were planted in pots containing 100g of gardening soil, and 3 pieces of them were made at intervals of 2m in the field, and 30 pieces of mustard seeds per group were placed at intervals of 30㎝. Then, water was sprayed on the mustard radish between 10:00 am and 11:00 am on a sunny day at a temperature of 25 to 27 ° C and then wetted with water. Then, the gingko leaf water-aged material of Example 3, the aged ginkgo leaf of Example 4, The ginkgo leaf water-aged material of Comparative Example 3, the ginkgo leaf non-aging material of Comparative Example 4, the powder of the ginkgo leaf non-aging material and the mull bean fruit powder of Comparative Example 5, the powder of Comparative Example 6 3g each of beads powder was sprayed using a powder pesticide sprayer. Thereafter, the powders were sprayed in the same manner twice more at intervals of two days. After 7 days of last spraying, the state of each mustard radish was confirmed and the results are shown in Table 8 below.

Condition Number of edible canards (mustard) Example 3-1 24 Example 3-2 24 Example 3-3 24 Example 3-4 25 Example 3-5 26 Examples 3-6 27 Examples 3-7 28 Examples 3-8 23 Examples 3-9 26 Examples 3-10 25 Examples 3-11 27 Examples 3-12 28 Examples 3-13 26 Examples 3-14 27 Examples 3-15 25 Examples 3-16 27 Examples 3-17 26 Examples 3-18 26 Examples 3-19 25 Examples 3-20 25 Examples 3-21 24 Example 4-1 30 Example 4-2 30 Comparative Example 3-1 3 Comparative Example 3-2 4 Comparative Example 3-3 2 Comparative Example 3-4 3 Comparative Example 3-5 18 Comparative Example 3-6 4 Comparative Example 3-7 5 Comparative Example 3-8 6 Comparative Example 3-9 5 Comparative Example 3-10 19 Comparative Example 3-11 3 Comparative Examples 3-12 2 Comparative Example 3-13 3 Comparative Example 3-14 4 Comparative Example 3-15 19 Comparative Example 3-16 2 Comparative Example 3-17 One Comparative Example 3-18 2 Comparative Example 3-19 One Comparative Example 3-20 One Comparative Example 3-21 2 Comparative Example 3-22 5 Comparative Example 3-23 4 Comparative Example 3-24 3 Comparative Example 3-25 5 Comparative Example 3-26 19 Comparative Example 3-27 2 Comparative Example 3-28 4 Comparative Example 4-1 5 Comparative Example 4-2 6 Comparative Example 4-3 3 Comparative Example 5 2 Comparative Example 6 One Control group One

    As can be seen from the results of Table 8, it was found that the control effect of the ginkgo biloba aged material of the present invention against mustard radish was remarkably better than those of the control and control groups, Powder mixed with water and mulberry powder showed the best control effect.

< Experimental Example  5. Ginkgo leaf water Aging water  Identification of Plant Growth Enhancement and Insecticidal Effect of Fertilizer Composition Containing>

The ginkgo biloba aged material of Example 3, the ginkgo biloba powder of Example 4, the ginkgo leaf aged material of Comparative Example 3, the ginkgo leaf non-aging material of Comparative Example 4, and the ginkgo leaf of Comparative Example 5 5 g of silicic acid, 5 g of the compound fertilizer and 30 g of the organic fertilizer were mixed with 50 g of the powder mixed with the non-aged water and the mull bean fruit powder, and 50 g of the mull bean fruit powder of the comparative example 6. The mixture was put into a dryer at 30-40 ^° C, Lt; / RTI &gt;

On the other hand, the above-prepared fertilizer was mixed into the pot containing the soil for horticulture (10g of the above fertilizer was mixed per 100g of the soil of the pot), and after 4 days, 4 pairs of mustard seeds were planted in each pot. After that, three blocks were made at intervals of 2m in the field, and 20 pieces of mustard seeds were placed at intervals of 30 cm for each group. The condition of mustard radish was observed for 20 days under the condition that the temperature was kept at 25 to 27 ° C, and the results are shown in Table 9 below.

Composition to be included in compost Number of edible canards (mustard) Average weight per discard of mustard radish leaves Example 3-1 13 155.6 Example 3-2 15 158.4 Example 3-3 13 155.5 Example 3-4 16 156.7 Example 3-5 14 153.8 Examples 3-6 14 158.9 Examples 3-7 16 159.6 Examples 3-8 12 166.7 Examples 3-9 17 157.8 Examples 3-10 15 159.9 Examples 3-11 14 150.6 Examples 3-12 16 156.7 Examples 3-13 15 157.8 Examples 3-14 18 158.5 Examples 3-15 15 155.4 Examples 3-16 18 154.5 Examples 3-17 18 155.6 Examples 3-18 13 156.7 Examples 3-19 17 153.8 Examples 3-20 18 152.5 Examples 3-21 18 153.6 Example 4-1 20 184.7 Example 4-2 20 188.8 Comparative Example 3-1 One 118.7 Comparative Example 3-2 3 115.5 Comparative Example 3-3 One 114.6 Comparative Example 3-4 2 127.8 Comparative Example 3-5 4 122.4 Comparative Example 3-6 9 112.5 Comparative Example 3-7 4 126.6 Comparative Example 3-8 4 127.7 Comparative Example 3-9 6 118.4 Comparative Example 3-10 8 119.5 Comparative Example 3-11 2 115.6 Comparative Examples 3-12 One 116.7 Comparative Example 3-13 2 127.4 Comparative Example 3-14 3 124.7 Comparative Example 3-15 9 125.8 Comparative Example 3-16 One 126.4 Comparative Example 3-17 2 128.5 Comparative Example 3-18 3 126.6 Comparative Example 3-19 2 118.4 Comparative Example 3-20 One 125.5 Comparative Example 3-21 2 124.7 Comparative Example 3-22 3 125.4 Comparative Example 3-23 4 115.5 Comparative Example 3-24 2 128.6 Comparative Example 3-25 6 129.8 Comparative Example 3-26 9 126.5 Comparative Example 3-27 One 117.6 Comparative Example 3-28 3 128.7 Comparative Example 4-1 2 125.5 Comparative Example 4-2 4 126.6 Comparative Example 4-3 2 114.8 Comparative Example 5 2 115.9 Comparative Example 6 One 126.6 Control group (compost only) 0 115.7 Untreated group 0 92.9

As shown in Table 9, when the fertilizer composition containing ginkgo biloba aged material was treated with mustard radish, the population of mustard radish was preserved similarly to the case where the ginkgo biloba aged material was directly coated with the insecticide composition, It was confirmed that the insecticidal effect was still maintained.

In addition, since it was confirmed that the fertilizer composition having different weight per aeration of the mustard leaves treated with the fertilizer composition of the present invention had a remarkable weight increase as compared with the treated mustard seeds, The effect was also excellent.

< Experimental Example  6. Ginkgo leaf water Aging water  Identification of the insecticidal effect of the contained fertilizer composition &gt;

The ginkgo biloba aged material of Example 3-8, the powder of the ginkgo leaf aged material of Example 4-1 and the powder of the far field bead powder, the ginkgo leaf aged material of Comparative Example 3-22, the ginkgo leaf beverage of Comparative Example 4-1 aging water, Comparative example 5 non-aged Ginkgo water and mullite beads fruit powder is a mixed powder of Comparative example 6 far beads fruit powder silicate ssikeul 50g each 5g, 5g of compound fertilizer and 30 to 40 it is mixed with organic fertilizer 30g of ^o C &lt; / RTI &gt; dryer and dried under nitrogen with stirring. Next, the above-mentioned fertilizers were cultivated 2 times in a ginseng field of 100 m 2 in the second year of cultivation at intervals of 15 days (including 0.3 g per 1 m 2 each). Two months after the soil cultivation, the pest of the pests remaining in the soil of the ginseng field was selected and the population was confirmed. The results are shown in Table 10 below. Gryllotalpa orientalis ) is an insect in the papillary and caterpillars, widely found in Asia and Australia, is a soil pest that feeds on the roots of crops and causes the roots of oyster plants to thrill and die.

Cultivated crop
Fertilizer type 100 square meters Number of puppies confirmed in cultivated area (grains)



Ginseng The fertilizer containing the ginkgo leaf aged material of Example 3-8 One Fertilizer containing powder mixed with ginkgo biloba fruit and mulberry beverage powder of Example 4-1 0 The ginkgo leaf of Comparative Example 3-22 10 Fertilizer containing ginkgo biloba unripe substance of Comparative Example 4-1 11 The ginkgo leaf of Comparative Example 5 and the fertilizer containing powder mixed with non-aged water and mulberry fruit powder 12 The fertilizer containing the mull bean fruit powder of Comparative Example 6 13 Untreated group 15

As shown in Table 10, it can be seen that the ginseng cultivated with soil containing the fertilizer containing the ginkgo biloba aged material according to the present invention significantly reduced the population of grenades confirmed in the cultivation area of 100 m 2.

< Experimental Example  7. Ginkgo leaves Aging Fungicide  Checking effects>

In order to confirm the antifungal effect of the ginkgo leaf agar of the present invention, the effect of controlling the wilt disease (causative organism: fusarium oxysporum) on strawberry (macaque variety) in seedling packing was investigated. At this time, cultivated soils in which wilt disease bacteria were identified were used as soil for seedling packing. The control using the ginkgo leaf aged water was started after 1 month after the strawberry seedlings were planted.

One month after the seedling of the strawberry seedlings was dipped in 30 drops per group, water was sprayed on the seedlings and wetted, and then the aging of the ginkgo biloba of Example 3-8, the powder of the ginkgo biloba fruit powder of Example 4-1, The aged ginkgo leaf material of Comparative Example 3-22, the non-aged ginkgo leaf of Comparative Example 4-1, the powder of the ginkgo leaf non-aging material and the merle bead fruit powder of Comparative Example 5, and the merle bean fruit powder of Comparative Example 6 Were sprayed with a powdered agricultural chemical sprayer. Thereafter, the powders were sprayed in the same manner twice more at intervals of two days. After 7 days of last spraying, the state of the strawberry was confirmed and the results are shown in Table 11 below.

Cultivated crop process Control (%)


Strawberry The ginkgo leaf aged material of Example 3-8 92.6 Powder mixed with the ginkgo biloba fruit of Example 4-1 and the bead fruit powder 89.6 The ginkgo biloba aged material of Comparative Example 3-22 24.6 The ginkgo leaf of Comparative Example 4-1 23.6 The powder of the ginkgo leaf of Comparative Example 5 mixed with non-aged water and mull bean fruit powder 24.6 The mull bean fruit powder of Comparative Example 6 38.6 Untreated group -

From the results of Table 11, it was confirmed that the composition containing the ginkgo biloba of the present invention had a high control effect.

< Experimental Example  8. Ginkgo leaves Aging water  Of the contained fertilizer composition Fungicide  Checking effects>

Experimental Example  8-1. Wilted (Causative bacteria: Fuarium Oxyspolom ) Control effect

In order to confirm the antifungal effect of the fertilizer composition containing the ginkgo leaf agar according to the present invention, the effect of controlling the worm disease (causative organism: fusarium oxysporum) on strawberry (pine varieties) was examined in seedling packing. At this time, cultivated soils in which wilt disease bacteria were identified were used as soil for seedling packing. The control using the ginkgo leaf aged water was started after 1 month after the strawberry seedlings were planted.

To this end, the aged ginkgo biloba seeds of Example 3-8, the ginkgo leaf aged material of Example 4-1 and the powder of mull bean fruit powder, the ginkgo leaf aged material of Comparative Example 3-22, -1 ginkgol, 5 g of silicic acid, 5 g of the compound fertilizer and 30 g of the organic fertilizer were mixed with 50 g of each of the non-aged ginkgo leaf of Comparative Example 5 and the ginkgo green powder of Comparative Example 5 and the mulberry beverage powder of Comparative Example 6, It was placed in a dryer at 30-40 ^° C and dried under nitrogen with stirring.

Thereafter, the fertilizer was applied to the harvesting machine 3 times at intervals of 10 days (1 g / strawberry 1 week), and the control was observed at the harvest time. The copper hydroxide (77%) was used as a control agent and the control agent was shown in Table 12.

Cultivated crop process Control (%)



Strawberry The fertilizer containing the ginkgo leaf aged material of Example 3-8 92.6 Fertilizer containing powder mixed with ginkgo biloba fruit and mulberry beverage powder of Example 4-1 89.6 The ginkgo leaf of Comparative Example 3-22 24.6 Fertilizer containing ginkgo biloba unripe substance of Comparative Example 4-1 23.6 The ginkgo leaf of Comparative Example 5 and the fertilizer containing powder mixed with non-aged water and mulberry fruit powder 24.6 The fertilizer containing the mull bean fruit powder of Comparative Example 6 38.6 Cooper hydrate 65.2 Untreated group -

From the results of Table 12, it can be said that the fertilizer composition containing the ginkgo biloba of the present invention shows a better control effect than the cooper hydrate, which is the comparative agent, and can replace the chemical pesticide disclosed in strawberry wilt.

Experimental Example  8-2. Root rot disease (Causative bacteria: Cylindrophone Death Trucks ) Control effect

In order to confirm the antifungal effect of the fertilizer composition containing the ginkgo leaf agar according to the present invention, ginseng root rot disease inhibitory activity was investigated in ginseng cultivated area.

The ginkgo biloba aged material of Example 3-8, the powder of the ginkgo leaf aged material of Example 4-1 and the powder of the far field bead powder, the ginkgo leaf aged material of Comparative Example 3-22, the ginkgo leaf beverage of Comparative Example 4-1 aging water, Comparative example 5 non-aged Ginkgo water and mullite beads fruit powder is a mixed powder of Comparative example 6 far beads fruit powder silicate ssikeul 50g each 5g, 5g of compound fertilizer and 30 to 40 it is mixed with organic fertilizer 30g of ^o C &lt; / RTI &gt; dryer and dried under nitrogen with stirring.

The presence of root rot bacteria was confirmed in the cultivated ginseng cultivated in the afternoon, and 2 years old ginseng was transplanted in one of 5,000 cultivated areas (Note: The size of one car was 1.8 × 0.9m, and the number of ginseng used in the test was 500 roots ). After that, the fertilizer was sprayed on the cultivated soil where the root rot fungus was present (each containing 0.3 g per 1 m 2), and the 2-year-old ginseng was transferred and planted. After 3 months, the ginseng root was germinated and the degree of improvement of root rot disease of ginseng was examined for each cultivation treatment treatment.

Cultivated crop process Root rot inhibition rate (%)



Ginseng The fertilizer containing the ginkgo leaf aged material of Example 3-8 96.6 Fertilizer containing powder mixed with ginkgo biloba fruit and mulberry beverage powder of Example 4-1 96.5 The ginkgo leaf of Comparative Example 3-22 24.4 Fertilizer containing ginkgo biloba unripe substance of Comparative Example 4-1 35.5 The ginkgo leaf of Comparative Example 5 and the fertilizer containing powder mixed with non-aged water and mulberry fruit powder 26.2 The fertilizer containing the mull bean fruit powder of Comparative Example 6 4.3 Untreated group -

As shown in Table 13, the roots of the untreated group remained intact, and it was confirmed that the ginseng roots of the soil sprayed with the fertilizer containing the ginkgo biloba syrup of the present invention improved root rot, .

< Experimental Example  9. Ginkgo leaves Aging  Check for antiviral effects>

Tomato "Unicorn" seedlings were planted in 100 square meters of each field affected by mosaic virus. After one month, water was sprayed and wetted well, and then aged ginkgo biloba of Example 3-8, the mixture of ginkgo biloba powder and merle bead fruit powder of Example 4-1, and the ginkgo biloba of Comparative Example 3-22 Water, non-aged gingko leaf of Comparative Example 4-1, 3 g of each of the powder of the ginkgo leaf non-aged material of Comparative Example 5 and the powder of the mulberry beverage powder of Comparative Example 6, and the powder of the mulberry beverage of Comparative Example 6 were sprayed using a powder pesticide sprayer . Thereafter, the powders were sprayed in the same manner twice more at intervals of two days. After 7 days of the last spraying, the state of the tomato was confirmed and the results are shown in Table 14 below.

Cultivated crop
Fertilizer type Percentage of non-intact mosaic viruses (%)


tomato The ginkgo leaf aged material of Example 3-8 89.6 The ginkgo leaf aged material of Example 4-1 79.2 The ginkgo biloba aged material of Comparative Example 3-22 33.6 The ginkgo leaf of Comparative Example 4-1 29.3 The powder of the ginkgo leaf of Comparative Example 5 mixed with non-aged water and mull bean fruit powder 26.4 The fertilizer containing the mull bean fruit powder of Comparative Example 6 35.2 Untreated group 24.7

< Experimental Example  10. Ginkgo leaves Aging water  Determination of the antiviral effect of the contained fertilizer composition >

The ginkgo biloba aged material of Example 3-8, the powder of the ginkgo leaf aged material of Example 4-1 and the powder of the far field bead powder, the ginkgo leaf aged material of Comparative Example 3-22, the ginkgo leaf beverage of Comparative Example 4-1 5 g of silicic acid, 5 g of the compound fertilizer and 30 g of the organic fertilizer were mixed with 50 g of each of the aged material, the mixture of the non-aged ginkgo leaf and the mull bean fruit powder of Comparative Example 5 and the mull bean fruit powder of Comparative Example 6, ^o C &lt; / RTI &gt; dryer and dried under nitrogen with stirring. Next, it was confirmed whether the fertilizer had an antiviral effect against the mosaic virus of each plant.

For this purpose, tomatoes, cucumbers and lettuce seedlings were cultivated in 100 square meters of each field affected by mosaic virus. Formulas were made at the most appropriate formal period known for each crop, and tomatoes 'unicorn', 'cucumber' and 'lettuce' were used for each crop variety. After the formulation, the fertilizers were first applied to the tomatoes, cucumbers and lettuce after 15 days after the formulation, and thereafter sprayed with the soil at intervals of 15 days (each containing 0.3 g per 1 m 2).

On the other hand, the official timing, harvesting time and the number of times of spraying of the diluted fertilizer are as follows.

Tomatoes: April 1, 2011 formal, June 1, 2011 harvest, 4 times spray.

Lettuce September 1, 2011 formal, October 1, 2011 harvest, sprayed twice.

Cucumber July 1, 2011 Formal, August 1, 2011 Harvest, spray twice.

Two months later, the status of each crop against mosaic virus is shown in Table 15 below.

Cultivated crop
Fertilizer type Percentage of non-intact mosaic viruses (%)



tomato The fertilizer containing the ginkgo leaf aged material of Example 3-8 92.8 Fertilizer containing powder mixed with ginkgo biloba fruit and mulberry beverage powder of Example 4-1 85.4 The ginkgo leaf of Comparative Example 3-22 37.6 Fertilizer containing ginkgo biloba unripe substance of Comparative Example 4-1 39.3 The ginkgo leaf of Comparative Example 5 and the fertilizer containing powder mixed with non-aged water and mulberry fruit powder 31.4 The fertilizer containing the mull bean fruit powder of Comparative Example 6 35.7 Untreated group 27.2



cucumber The fertilizer containing the ginkgo leaf aged material of Example 3-8 92.4 Fertilizer containing powder mixed with ginkgo biloba fruit and mulberry beverage powder of Example 4-1 82.7 The ginkgo leaf of Comparative Example 3-22 36.3 Fertilizer containing ginkgo biloba unripe substance of Comparative Example 4-1 40.4 The ginkgo leaf of Comparative Example 5 and the fertilizer containing powder mixed with non-aged water and mulberry fruit powder 31.7 The fertilizer containing the mull bean fruit powder of Comparative Example 6 25.4 Untreated group 45.3



Lettuce The fertilizer containing the ginkgo leaf aged material of Example 3-8 90.5 Fertilizer containing powder mixed with ginkgo biloba fruit and mulberry beverage powder of Example 4-1 81.7 The ginkgo leaf of Comparative Example 3-22 36.3 Fertilizer containing ginkgo biloba unripe substance of Comparative Example 4-1 30.4 The ginkgo leaf of Comparative Example 5 and the fertilizer containing powder mixed with non-aged water and mulberry fruit powder 35.5 The fertilizer containing the mull bean fruit powder of Comparative Example 6 32.6 Untreated group 21.3

As a result of confirming the state of each crop after 2 months from the last soil crossing, it was confirmed that the composition of the present invention containing the ginkgo leaf aged material was antiviral effect against mosaic virus in tomato, cucumber and lettuce, Was high.

Claims (9)

A pesticide, fungicide or antiviral composition comprising ginkgo biloba water ripened at 25 to 50 ° C. by adding water to a ginkgo leaf powder having a particle size of 100 to 300 μm. The method of claim 1,
The ginkgo biloba water aging is added to 100 to 500 parts by weight of water to 100 parts by weight of ginkgo biloba powder having a particle size of 100 ~ 300μm, aging at 25 ~ 50 ℃ characterized in that the pesticide, fungicide or antiviral composition. The method of claim 1,
The ginkgo biloba water aging is a pesticide, fungicide, characterized in that the powder obtained by drying the reaction mixture prepared by aging the mixture of ginkgo biloba powder and water at 25 ~ 50 ℃, containing water and ginkgo biloba powder as it is Or antiviral agent compositions. The method of claim 1,
The ginkgo biloba water aging is a pesticide, fungicide or antiviral composition, characterized in that the liquid obtained after removing the powder from the reactant or a dry dried composition. The method of claim 1,
Wherein the composition further comprises a mull bean fruit powder. &Lt; RTI ID = 0.0 &gt; 11. &lt; / RTI &gt; The method of claim 1,
Wherein the composition further comprises a plant nutrient, an agrochemical or a mixture of two or more thereof. The method of claim 1,
The composition may further comprise at least one composition selected from the group consisting of aphids, aphids, black aphids, peach aphids, cotton aphids, spotted mites, greenhouse flour, thrips, clematis, gulls, moths, moths, Mulberry moths, rice moths moths, rice moths moths, rice moths moths, rice weevils, rice weevils, cabbage moths, cabbage avalanche, tobacco moths, At least one insect pest selected from the group consisting of a moth, a moth, a moth, a moth larva, a charcoal black chickpea, a gerbil room, a spotfly, a root mite, a rootstock nematode, a soil insect and a soil nematode, Fusarium oxysporum, Cylindrocarpon destructans, Botrytis cinerea, Ashley Bear, and so on. Colletotrichum gloeosporioides, Sclerotium cepivorum, Sclerotinia sclerotiorum ( Sclerotinia sclerotiorum ), Sclerotinia sclerotiorum ( Sclerotinia sclerotiorum ), Sclerotinia sclerotiorum , Penicillium hirsutum (causative organism of blue mold), Pectobacterium carotovorum (causal organism causative organism), Rhizoctonia solani (causal organism causative organism), Pisumthum At least one phytotoxic strain selected from the group consisting of Pythium ultimum , Pythium debaryanum and Rhizopus sp., A causative agent for acne , or a plant strain selected from the group consisting of tobacco Tobacco mosaic virus, cucumber mosaic virus, and potato virus Y To be used for controlling more than one kinds selected plant disease virus agent according to claim insecticides, fungicides or antiviral composition. A fertilizer composition comprising the composition of claim 1. Preparing gingko leaf as a powder having a particle size of 100 to 300 mu m;
Mixing 100 to 500 parts by weight of water with 100 parts by weight of the ginkgo leaf powder; And
Aging the ginkgo biloba powder and water mixture at 25˜50 ° C .;
&Lt; / RTI &gt; characterized in that the ginkgo biloba is aged.

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