KR100415107B1 - Ginko biloba extract and preparing methods and use thereof - Google Patents
Ginko biloba extract and preparing methods and use thereof Download PDFInfo
- Publication number
- KR100415107B1 KR100415107B1 KR10-2001-0010760A KR20010010760A KR100415107B1 KR 100415107 B1 KR100415107 B1 KR 100415107B1 KR 20010010760 A KR20010010760 A KR 20010010760A KR 100415107 B1 KR100415107 B1 KR 100415107B1
- Authority
- KR
- South Korea
- Prior art keywords
- ginkgo biloba
- extract
- insecticidal
- antibacterial
- weight
- Prior art date
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- NQJGJBLOXXIGHL-UHFFFAOYSA-N podocarpusflavone A Natural products COc1ccc(cc1)C2=CC(=O)c3c(O)cc(O)c(c3O2)c4cc(ccc4O)C5=COc6cc(O)cc(O)c6C5=O NQJGJBLOXXIGHL-UHFFFAOYSA-N 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- IKGXIBQEEMLURG-NVPNHPEKSA-N rutin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-NVPNHPEKSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 231100000004 severe toxicity Toxicity 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000004071 soot Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000011232 storage material Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- VXQYICLHHMETFH-UHFFFAOYSA-N tetra-O-methylamentoflavone Natural products C1=CC(OC)=CC=C1C1=CC(=O)C2=C(O)C=C(OC)C(C=3C(=CC=C(C=3)C=3OC4=CC(OC)=CC(O)=C4C(=O)C=3)OC)=C2O1 VXQYICLHHMETFH-UHFFFAOYSA-N 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 229960000984 tocofersolan Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000002076 α-tocopherol Substances 0.000 description 1
- 235000004835 α-tocopherol Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
- D21H21/14—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties characterised by function or properties in or on the paper
- D21H21/36—Biocidal agents, e.g. fungicidal, bactericidal, insecticidal agents
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 은행잎 추출물과 그 제조방법 및 용도에 관한 것으로서, 더욱 상세하게는 은행잎을 물로 추출하거나 알코올 및 헵탄으로 추출하여 플라본글리코사이드류, 테르펜락톤류 및 징코릭 애시드류를 소정량 함유하는 성분으로 추출한 추출물함으로서, 이러한 은행잎 추출물을 항균제, 살균(살충)제 또는 해충기피제 등과 같은 농약으로 사용하는 경우 인체에 전혀 유해하지 아니하고 우수한 효과를 나타내는 새로운 천연 조성의 은행잎 추출물과 그 제조 방법 및 용도에 관한 것이다.The present invention relates to a ginkgo biloba extract and a method and a use thereof, and more particularly, to extract a ginkgo biloba leaf with water or an alcohol and heptane to flavone glycosides, terpene lactones and ginkgolic acid as a component containing a predetermined amount By extracting the extract, the present invention relates to a ginkgo biloba leaf extract having a new natural composition, which is not harmful to the human body and exhibits excellent effects when used as a pesticide such as an antibacterial agent, a bactericidal (insecticide) or a pest repellent agent, and a method and a use thereof. .
Description
본 발명은 은행잎 추출물과 그 제조방법 및 용도에 관한 것으로서, 더욱 상세하게는 생은행잎을 물로 추출하거나 알코올 및 헵탄으로 추출하여 플라본글리코사이드류, 테르펜락톤류 및 징코릭 애시드류(Ginkgolic acid)를 소정량 함유하는 성분으로 추출한 추출물함으로서, 이러한 은행잎 추출물을 항균제, 살균제 또는 해충기피제로 사용하는 경우 우수한 효과를 나타내는 새로운 천연 조성의 은행잎 추출물과 그 제조 방법 및 용도에 관한 것이다.The present invention relates to a ginkgo biloba extract and a method for producing the same, and more particularly, to extract flavone glycosides, terpene lactones and ginkgolic acid by extracting fresh ginkgo biloba leaves with water or with alcohol and heptane. By extracting with a quantitative-containing component, the ginkgo biloba extract relates to a new natural composition ginkgo biloba extract exhibiting excellent effects when used as an antibacterial, bactericidal or pest repellent, and a method and use of the same.
농업생산성 증대와 농산물 장기저장에 농약은 커다란 공헌을 해왔고 사용량도 꾸준히 증가하고 있다. 일반적인 유기약제 농약이 지니고 있는 단점의 하나인 저항성해충의 출현과 더불어 약제를 살포하는 회수의 증가와 희석농도의 증가로 인하여 해충방제효과는 점점 감소되고, 반면에 천적들은 치명적인 타격을 받아 잠재적 곤충의 해충화에 따른 새로운 해충의 출현이 많아지고 있다(해충학, 백운하, 1999). 또한 농산물의 잔류 농약량의 증가로 농산물의 독성이 증가되어 식품에 대한 부정적 요인으로 작용하고 있다(이서래, 1982).Pesticides have contributed greatly to increasing agricultural productivity and long-term storage of agricultural products, and their consumption has been steadily increasing. In addition to the emergence of resistant pests, one of the disadvantages of conventional organic chemical pesticides, the increase in the number of spraying agents and the increase in dilution concentration, the pest control effect is gradually reduced, while the natural enemies are lethal blows. The emergence of new pests following pestization is increasing (Pestology, Baiyun Hae, 1999). In addition, the increase in the amount of residual pesticides in agricultural products increases the toxicity of agricultural products, thus acting as a negative factor for food (Lee Seo-rae, 1982).
농약에 대한 피해는 토양의 오염과 생태계의 교란, 환경호르몬으로서의 인체에 대한 피해는 꾸준히 증가하여 위험수위에 이르렀다. 특히, 살충, 살균 제초제 등의 농약은 유기염소계, 유기인산계, 카바메이트계 등이 주종을 이루며 이들 모두 심각한 독성과 낮은 분해성으로 인하여 생태계에서도 먹이사슬에 의하여 농축되는 효과를 가져오는 것으로 알려지고 있다(이규승, 1997). 따라서, 화학제 농약으로 병충해를 막는 것은 환경측면에서는 한계에 이르렀으며 농산품의 안전성측면에서도 심각한 위협이 되고 있다(이서래, 1982).The damage to pesticides has reached the level of danger as soil pollution, ecosystem disturbance, and damage to human body as environmental hormones have steadily increased. In particular, pesticides such as insecticides and bactericidal herbicides are mainly composed of organic chlorine, organic phosphate, and carbamate, all of which are known to have the effect of being concentrated by the food chain in the ecosystem due to severe toxicity and low degradability. (Lee Kyu-seung, 1997). Therefore, preventing pests with chemical pesticides has reached the limit in terms of environment and poses a serious threat in terms of safety of agricultural products (Lee Seo-rae, 1982).
일반적으로 농약의 중독현상에는 부적절한 사용에 의한 농약 중독, 농부의 중독, 사고에 의한 중독, 만성 농약중독 등이 있으나, 이중에서도 만성 농약중독에 따른 문제점은 많은 학자들이 장기적으로 다양한 종류의 암을 유발시킬 수 있다고 보고되고 있다(김용화, 1980). 이에 따라 생물학적 방제가 최근 미생물을 중심으로 이루어지고 있으나 미생물 제재의 경우 취급하기가 용이하지 않아 상업화의 장애가 되고 있다(임성열, 1995).Generally, poisoning of pesticides includes pesticide poisoning due to improper use, farmer poisoning, accidental poisoning, and chronic pesticide poisoning. Among them, the problem of chronic pesticide poisoning is that many scholars cause various types of cancer in the long term. It is reported that it can be done (Kim Yong-hwa, 1980). As a result, biological control has recently been centered on microorganisms, but microbial preparations are not easy to handle and thus hinder commercialization (Lim Sung-yeol, 1995).
따라서, 인간과 동식물에게는 무해한 환경친화적이고 취급이 용이하며 생산가격이 낮은 상품화가 이루어질 수 있는 천연물 농약이 요구되고 있는 바, 우리 주변에서 쉽게 구할 수 있는 식물 중에서 은행나무는 약 2억년전 삼첩기(Triassic)말기나 쥬라기(Jurassic)초기부터 지구상에 존재해온 Gink-goales 로 알려진 식물군중 유일하게 현존하는 종이며 다아윈(Darwin)은 이것을 "살아있는 화석"이라 칭하기도 하였다.Therefore, natural products and pesticides that are harmless to humans and animals, which are harmless to the environment, easy to handle, and low in production price, are required. Among the plants readily available around us, Ginkgo biloba is about 200 million years ago. It is the only living species known as Gink-goales that has existed on Earth since the end of the Jurassic period or early Jurassic, and Darwin called it "living fossils."
징코빌라바(Ginkgo Biloba) 나무군들은 페르미안(Permian)시대 동안에 고대양치류 종자로부터 진화된 것으로, 빙하기 이후의 대변동 3기와 4기 동안에 동아시아를 제외한 모든 지역에서 은행나무와 다른 Ginkgoales가 자취를 감추게 된 것으로 추정된다(최낙성 1998). 은행나무잎 추출물은 세계적으로 중요한 약품의 원료가 되었고 그 사용량도 꾸준히 증가하는 추세이다. 은행나무의 효용성이 높아감에 따라 은행나무에 대한 연구가 폭발적으로 증가하였다. 또한 은행나무는 어떻게 오랜 세월이 지나는 동안 변화 및 변이(mutation)없이 유지될 수 있었는가 하는 의문점에 대한 부르크하벤(Brookhaven) 국립연구소의 A. H. Sparrow 박사의 탐구결과 화학물질이 은행나무 유전자(gene)의 변이를 일으킬 수도 있으나 은행나무의 생육주기(generation)가 상대적으로 길기 때문에 변이가 최소화되어 진다는 것을 보고하였다. 즉, 은행나무는 20년 이상이 되어야만 번식을 위한 재생산을 할 수 있으며 이것은 1000년 이상 지속된다. 결론적으로 은행나무는 재생산(reproduction) 기간이 짧은 다른 식물군에 비해 그 특징을 변화시킬 수 있는 기회가 적다는 것이다. 또한 은행나무는 모든 심한 해충들과 질병들에 저항성이 강하며 도시의 스모그와 매연등의 산업공해에 대해서 높은 저항성을 가지고 있는 식물로 평가받고 있다. 이러한 결과를 입증할 만한 자료는 은행나무 뿌리의 알콜추출물(alcoholic extracts)이 유럽의 옥수수좀인 Pyranstamubilatis 유충의 성장을 강하게 저해하는 것이 위스콘신대학의 S .D. Berk 교수에 의해 관찰되었으며, 버지니아대의 R .T. Major 교수의 연구실에서 관찰한 결과에 따르면 일본 딱정벌레는 은행나무의 잎을 갉아먹는 것보다 아사 쪽을 택하는 것으로 알려졌다. 또한, Boyce Thompson 식물연구소와의 공동연구결과 은행잎의 아세톤 추출물이 식물체에 손상을 입히는Erwinia Amylovora, E.coli, P. phaseolicola, Xanthomonas phaseoli, B. Pumilus등의 세균 성장을 정지시키는 것으로 관찰 되었고, 뿌리의 알콜 추출물은 강남콩 모자이크바이러스(Southern Bean Mosaic Virus) 와 담배 모자이크 바이러스(Tabaco Mosaic Virus)의 감염증상이 저해됨을 J. W. Mitchell등이 밝혀내었으며, 곰팡이에 대한 저항성 연구결과 2-헥세날(2-hexenal)과 비방향족 하이드로카본(nonaromatic hydrocarbone)으로서 카보닐기를 가진 것으로 추정되는 오일이Monilinia fructicola등의 곰팡이의 생육을 크게 저하하는 것이 관찰되었다. 잎의 표피층에 있는 왁스물질은 곰팡이의 포자발아를 감소시키는 것으로 Johaston과 Sproston의 연구로 밝혀졌다. 그러나 은행잎 추출물을 사용하여 살충제로서의 기초실험결과나 개발은 아직 시도된 바가 거의 없고 국내외적으로 특허출원도 미미한 실정이다. 이러한 상황에서 은행잎 추출물을 이용한 살충제의 개발은 기술적 우위 측면이나 경제적 유발 효과 측면에서 매우 유용할 것으로 보여진다.Ginkgo Biloba tree families evolved from ancient fern seeds during the Permian era, where ginkgo biloba and other Ginkgoales disappeared in all regions except East Asia during the 3rd and 4th periods of the Cataclysm. It is assumed (Nakseong 1998). Ginkgo biloba extract has become an important ingredient in the world, and its usage is steadily increasing. As the effectiveness of ginkgo biloba grows, research on the gingko biloba has exploded. In addition, a study by Dr. AH Sparrow of the Brookhaven National Laboratory on the question of how ginkgo trees could be maintained without changes and mutations over the years has led to chemicals in the Ginkgo gene. Although it may cause mutations, it has been reported that the variation is minimized because of the relatively long generation of ginkgo biloba. In other words, ginkgo biloba must be over 20 years to reproduce for reproduction, which lasts more than 1000 years. In conclusion, ginkgo biloba has fewer opportunities to change its characteristics than other plant species with short reproduction periods. Ginkgo biloba is also highly resistant to all severe pests and diseases, and is highly regarded as a plant resistant to industrial pollution such as smog and soot in cities. Evidence suggests that alcoholic extracts of ginkgo roots strongly inhibit the growth of Pyranstamubilatis larvae, a European corn seed, by S.D. Observed by Professor Berk, R.T. Observations in Prof. Major's lab show that Japanese beetles prefer the Asa rather than gnawing leaves of ginkgo biloba. In addition, research with Boyce Thompson Botanical Research Institute showed that acetone extracts from ginkgo biloba inhibited the growth of bacteria such as Erwinia Amylovora, E. coli, P. phaseolicola, Xanthomonas phaseoli , and B. Pumilus, which damage plants. The alcohol extract of JW Mitchell et al. Found that the infection symptoms of Southern Bean Mosaic Virus and Tobacco Mosaic Virus were inhibited by JW Mitchell, et al. 2-Hexenal. ) And oils which are assumed to have carbonyl groups as nonaromatic hydrocarbons significantly reduced the growth of fungi such as Monilinia fructicola . Waxes in the epidermal layers of the leaves reduce the spore germination of the fungus, according to Johaston and Sproston. However, basic experiment results or development as a pesticide using ginkgo biloba extract have not been attempted yet, and the patent application is inadequate both at home and abroad. In this situation, the development of pesticides using ginkgo biloba extract is expected to be very useful in terms of technological superiority or economic effect.
종래, 은행잎 추출물을 살충제로 사용하는 기술로서는 한국특허등록 제273177호에서 은행잎을 알콜 수용액과 비극성용매를 사용하여 그 추출물을 활성탄으로 처리하여서 무공해 농약으로 이용할 수 있는 천연 살충성분 분확을 제조하는 방법에 대하여 발표하고 있다.Conventionally, as a technique of using ginkgo biloba extract as an insecticide, in Korean Patent Registration No. 273177, a method for producing a natural insecticidal component which can be used as a pollution-free pesticide by treating the extract with activated carbon using an aqueous alcohol solution and a nonpolar solvent. Is being announced.
그러나, 이러한 종래 기술에서는 활성탄 처리 등 그 공정이 비교적 복잡하고 제조된 후 성분도 일정한 분획으로 얻지 못하여 상업화하기에는 곤란한 문제가 있었다.However, such a prior art has a problem that the process such as activated carbon treatment is relatively complicated, and even after the manufacturing process, the components are not obtained in a constant fraction, which is difficult to commercialize.
이와 같이, 종래의 은행잎 추출물로부터 살충제를 제조하는 방법을 보다 효과적으로 개선하고 일정한 성분을 함유하는 분획으로 은행잎 추출물을 얻고 이를 특정의 성분 구성에 적합한 용도로 사용할 수 있도록 하여 무해한 천연성분의 농약을 상업화가 가능하도록 제조하는 방법이 절실히 요구되고 있다.In this way, the method of preparing pesticides from conventional ginkgo biloba extracts more effectively and obtaining ginkgo biloba extracts with fractions containing certain ingredients and allowing them to be used for specific composition of ingredients, commercializing harmless natural pesticides There is an urgent need for a method of making it possible.
따라서, 본 발명의 목적은 천연물의 일종이며 국내에서 쉽게 재료를 구할 수 있고 특정조성으로 유효성분을 함유하여 살충력과 살균력이 우수한 은행잎 추출물을 제공하는데 있다.Therefore, an object of the present invention is to provide a ginkgo biloba extract excellent in insecticidal and bactericidal properties by being a kind of natural and easy to obtain ingredients in the country and containing the active ingredient in a specific composition.
또한, 본 발명의 다른 목적은 특정 조성의 유효성분을 함유하여 살균, 살충력을 발휘하도록 은행잎 추출물을 상업적으로 제조하는 방법을 제공하는데 있다.In addition, another object of the present invention is to provide a method for commercially preparing a ginkgo biloba extract to contain the active ingredient of a specific composition to exert sterilization, insecticide.
또한, 본 발명의 또 다른 목적은 특정 조성의 유효성분을 가지는 은행잎 추출물을 환경친화적이면서도 인체에 무해한 살충·살균제 및 해충기피제(repellent) 등의 농약으로 사용하도록 하는 용도를 제공하는데 있다.In addition, another object of the present invention is to provide a ginkgo leaf extract having an active ingredient of a specific composition to be used as a pesticide, such as insecticides, fungicides and pest repellents that are environmentally friendly and harmless to the human body.
본 발명은 은행잎을 용매로 추출한 은행잎 추출물에 있어서, 은행잎 100중량부에 증류수 또는 유기용매 10 ~ 500 중량부를 가하여 믹서로 갈고 추출 분리한 액상성분으로서, 그 액상성분 중에 플라본글리코사이드가 2 ~ 5 중량%, 테르펜락톤이 0.01 ~ 1 중량%, 징콜릭애시드(Ginkgolic acid)가 3 ~ 30중량%로 함유되어 있는 것을 특징으로 한다.The present invention is a ginkgo biloba extract extracted from the ginkgo biloba extract as a solvent, 10 to 500 parts by weight of distilled water or an organic solvent is added to 100 parts by weight of ginkgo biloba as a liquid component extracted and separated by a mixer, the flavone glycoside 2 ~ 5 weight in the liquid component %, Terpenactone is 0.01 to 1% by weight, ginkgolic acid (Ginkgolic acid) is characterized in that it contains 3 to 30% by weight.
본 발명에서 상기 은행잎 추출물을 추출분리하기 위하여 단순히 필터 또는 원심분리하거나, 60 ~ 100℃에서 1 ~ 30분간 중탕하여 갈녹색 용액으로 추출하거나, 이를 에탄올 또는 메탄올과 n-헵탄을 동량의 중량비로 혼합한 용액으로 추출하고 이를 0.8 X 103Pa ~ 1 X 102Pa 사이에서 감압증류하여 추출한 것을 특징으로 한다.In the present invention, the extract of the ginkgo biloba extract is simply filtered or centrifuged, or extracted with brown green solution by agitation at 60-100 ° C. for 1-30 minutes, or mixing ethanol or methanol and n-heptane in the same weight ratio. Extracted as a solution and extracted by distillation under reduced pressure between 0.8 X 10 3 Pa ~ 1 X 10 2 Pa.
또한, 본 발명은 상기와 같은 방법으로 은행잎 추출물을 제조하는 방법을 포함한다.In addition, the present invention includes a method for producing a ginkgo biloba extract in the same manner as described above.
또한, 본 발명은 상기와 같이 제조한 은행잎 추출물을 유효성분으로 함유한 항균제, 살충제 또는 해충기피제를 포함한다.In addition, the present invention includes an antimicrobial agent, insecticide or insect repellent containing the ginkgo leaf extract prepared as described above as an active ingredient.
또한, 본 발명은 상기와 같이 제조된 은행잎 추출물을 유효성분으로 함유한 수경재배용 양액을 포함한다.In addition, the present invention includes a hydroponic cultivation solution containing the ginkgo leaf extract prepared as described above as an active ingredient.
또한, 본 발명은 상기와 같이 제조된 은행잎 추출물을 유효성분으로 함유한 과일 보관용제를 포함한다.In addition, the present invention includes a fruit storage solvent containing the ginkgo leaf extract prepared as described above as an active ingredient.
이와 같은 본 발명을 더욱 상세히 설명하면 다음과 같다.Referring to the present invention in more detail as follows.
본 발명은 은행잎으로부터 추출한 플라본글리코사이드류, 테르펜락톤류 및 징코릭 애시드류를 소정량 함유하는 추출물을 얻고 이를 특정의 용도로 상업화한 최초의 발명이다.The present invention is the first invention to obtain an extract containing a predetermined amount of flavone glycosides, terpenactones and ginkgolic acids extracted from ginkgo biloba and commercialize it for a specific use.
특히, 종래 일부 은행잎 용매 추출물을 단순히 살충용 농약으로 사용할 수 있다고 제안된 것은 단지 은행나무나 은행잎 고유의 특성으로 잘 알려진 해충, 박테리아, 바이러스 및 질병 저항성을 그대로 이용하려는 시도에 불과한 것이지 상업화를 위한 반복 대량 생산의 방법을 개발한 것은 아니다.In particular, it has been suggested that some conventional ginkgo biloba solvent extracts can be simply used as pesticides for pesticides, but are merely attempts to exploit the resistance to pests, bacteria, viruses, and diseases that are well known for their properties. The method of mass production was not developed.
본 발명에서는 은행잎으로부터 공업적 방법으로 유용한 다양한 방법으로 특정 조성의 추출물을 얻어내고 이러한 특정 조성의 추출물을 효과적인 특정 용도의 상품으로 제품화할 수 있는 실질적인 기틀을 마련한 것이다.In the present invention, the extract of a specific composition can be obtained from a ginkgo biloba by various methods useful for industrial methods, and the practical basis for producing the extract of the specific composition into a product for a specific use is effective.
본 발명에서 은행잎 추출물을 제조하기 위해서는 여러 방법이 제안될 수 있으나, 본 발명에서는 단순한 추출방법에서부터 지용성 성분까지 추출할 수 있는 모든 방법이 적용될 수 있으며, 이러한 추출방법은 특정의 성분 구성의 조성비를 가지는 추출물로 제조하는 방법이라면 어느 방법이나 본 발명에 포함된다.In the present invention, various methods may be proposed to prepare the ginkgo biloba extract, but in the present invention, any method capable of extracting from a simple extraction method to a fat-soluble component may be applied. Such an extraction method has a composition ratio of a specific component composition. Any method for producing an extract is included in the present invention.
본 발명에서는 항균, 살충, 해충기피 등의 효과를 나타내기 위해서는 특정의 성분 조성이 필요하다는 사실을 발명한 데 기초하고 있는 바, 본 발명에서 요구되는 은행잎 추출물의 성분 구성은 추출물인 액상성분 중에 적어도 플라본글리코사이드가 2 ~ 5 중량%, 테르펜 락톤이 0.01 ~ 1 중량%, 그리고 징콜릭애시드(Ginkgolic acid)가 3 ~ 30중량%로 함유되어 있는 것을 요구한다. 만일 상기 성분 중에서 어느 하나의 성분이 위 함량 범위보다 보족하면 본 발명의 목적을 달성할 수 없고, 그 이상으로 함유하는 경우는 크게 문제 될 것은 없으나 지나치게 비경제적이 방법으로 추출해야하는 등의 문제가 있어 바람직하지 않다. 또한, 함량이 위 보다 과량이면 특정 유해생물에 대한 저항성을 높여서 특정 용도로 사용하는데 적용할 수 있는 경우에 활용할 수 있다. 특히, 액상성분 중에 플라본글리코사이드가 함량이 너무 소량이면 용액의 친수성 및 항균, 살충효과의 저해의 문제가 있고 너무 과량이면 액제의 소수성이 증가하여 잔류성이 지속되고 식물의 잎과 줄기, 과일 표면에 코팅되는 효과를 갖는다. 테르펜 락톤은 해충에 대한 기피효과가 강한 특성의 물질로서, 너무 소량이면 다른 성분과의 상승효과(synergisitic effect)가 감소하고, 지나치게 과량이면 타성분의 효과를 감소시키는 역효과와 함께 액제의 불안정성의 용인으로 작용할 수 있다. 그리고, 징콜릭애시드(Ginkgolic acid)는 살충성이 강한 성분으로 특히 본 발명에서 이 성분이 너무 소량이거나 함유되어 있지 아니하면 살충제로의 문제를 기대할 수 없으므로 바람직하지 아니하고 너무 과량이면 피부 알러지를 유발하거나 독성으로 작용할 수 있다.In the present invention is based on the invention that the specific component composition is required to exhibit the effects of antibacterial, insecticide, insect repellent and the like, bar component composition of the ginkgo leaf extract required in the present invention is at least in the liquid component of the extract It is required to contain 2 to 5% by weight of flavone glycosides, 0.01 to 1% by weight of terpene lactones and 3 to 30% by weight of ginkgolic acid. If any one of the above components is more than the above content range, the object of the present invention can not be achieved, and if it contains more than that does not matter much, but there is a problem such as to be extracted in an uneconomical way Not desirable In addition, if the content is more than the above it can be utilized when it can be applied to use for a specific purpose by increasing the resistance to certain harmful organisms. In particular, if the amount of flavone glycoside in the liquid component is too small, there is a problem of inhibiting the hydrophilicity, antibacterial and insecticidal effect of the solution. If the amount is excessive, the hydrophobicity of the liquid is increased, so that the persistence is maintained and the leaves, stems and fruit surfaces of the plant are maintained. Has the effect of being coated. Terpene lactone is a substance that has a strong repelling effect against pests, and when too small, the synergisitic effect with other components is reduced, and when excessively excessive, an instability of the liquid agent is tolerated with an adverse effect that reduces the effects of other components. Can act as In addition, ginkgolic acid (Ginkgolic acid) is a highly insecticidal ingredient, especially in the present invention, if this component is too small or does not contain a problem as a pesticide is not desirable, too much excessively cause skin allergy or May act as toxic.
상기와 같은 본 발명의 추출 조성을 얻기 위해서는 예를 들어 생은행잎을 단순히 필터 또는 원심분리하거나, 60 ~ 100℃에서 1 ~ 30분간 중탕하여 갈녹색 용액으로 추출하거나, 이를 에탄올 또는 메탄올과 n-헵탄을 동량의 중량비로 혼합한 용액으로 추출하고 이를 0.8 X 103Pa ~ 1 X 102Pa 사이에서 감압증류하여 추출하는 방법 등으로 목적하는 추출물을 제조할 수 있다.In order to obtain the extraction composition of the present invention as described above, for example, raw ginkgo biloba leaves are simply filtered or centrifuged, or extracted with brown green solution by agitation at 60-100 ° C. for 1 to 30 minutes, or ethanol or methanol and n-heptane. The desired extract may be prepared by a method of extracting the solution by mixing the same amount by weight and distilling under reduced pressure between 0.8 X 10 3 Pa and 1 X 10 2 Pa.
이러한 추출방법 중에서 단순히 증류수로 추출하는 방법은 징콜릭애시드(Ginkgolic acid)의 함량이 비교적 적은 반면 그 추출공정이 간단하다는 잇점이 있다.Among these extraction methods, the method of simply extracting with distilled water has the advantage that the extraction process is simple while the content of ginkgolic acid is relatively small.
또한, 물로 추출 후 가온 중탕하는 방법은 징콜릭애시드의 함량을 더욱 증가한 형태의 추출물로 제조할 수 있으나, 다른 2가지 성분의 함량은 비슷한 결과를 얻을 수 있다.In addition, the method of extracting with warm water after extraction with water can be prepared as an extract of an increased form of the content of Jingkolic acid, the content of the other two components can obtain a similar result.
그리고, 물 추출물을 에탄올 또는 메탄올과 n-헵탄로 추출하고 감압증류하는 방법은 징콜릭애시드의 함량을 극대화시킬 수 있는 방법으로 볼 수 있는데, 이 경우는 3가지 성분을 모두 비교적 높게 얻을 수 있는 장점은 있지만 추출물의 유효함량이 높은 만큼 그 추출공정이 복잡하고 공정 조작이 까다롭다.In addition, the method of extracting the water extract with ethanol or methanol and n-heptane and distilling under reduced pressure can be seen as a method for maximizing the content of ginkgolic acid. In this case, all three components can be obtained relatively high. Although the effective content of the extract is high, the extraction process is complicated and difficult to operate.
상기와 같이 본 발명에 따라 제조된 은행잎 추출물은 플라본글리코사이드, 테르펜락톤, 징콜릭애시드를 주성분으로 하며 향균제 또는 살충제 또는 해충 기피제로 사용할 수 있는 항균, 살충 특성을 지닌 성분으로 확인되어 항균, 살충을 목적으로 하는 각종 제제로 사용할 수 있다. 즉, 본 발명의 은행잎 추출물은 그 추출방법에 따라 푸른색 생 은행잎, 노란색 생 은행잎, 건조 은행잎으로부터 추출법에 따라 성분비가 상이한 물질로서 각 성분비에 따라 항균제, 살충제, 기피제로 사용할 수 있다.The ginkgo biloba extract prepared according to the present invention as described above is composed of flavone glycoside, terpenlactone, ginkgolic acid as a main ingredient, and is identified as an ingredient having antibacterial and insecticidal properties that can be used as an antibacterial or insecticide or pest repellent. It can be used with the various preparations made into the objective. That is, the ginkgo biloba extract of the present invention may be used as an antimicrobial agent, an insecticide, or a repellent according to each component ratio as a substance having a different component ratio from the green raw ginkgo leaf, the yellow raw ginkgo leaf, and the dried ginkgo biloba according to the extraction method.
일반적으로 간단하게 증류수로 추출한 추출물은 예컨대 항균제 및 양액 수경재배에서 양액에 첨가하는데 사용하는 것이 바람직하다. 또한, 증류수로 중탕 추출한 추출물은 해충의 기피제 또는 살충제로 사용하는 것이 바람직하고, 물에 쉽게 용해되므로 과일의 보관용으로 사용하는 것이 바람직하다. 그리고, 알콜과 n-헵탄의 혼합용매로 추출한 추출물의 경우는 징콜릭애시드 함유량이 주성분으로 예컨대 살충제, 기피제로 사용할 수 있으며 직접 식물에 살포하는 야외 살포용으로 사용하기에 적합하다.In general, the extract simply extracted with distilled water is preferably used to add to the nutrient solution, for example in antibacterial and nutrient solution hydroponic culture. In addition, the extract extracted with boiling water with distilled water is preferably used as a repellent or insecticide of pests, and is preferably used for storage of fruits since it is easily dissolved in water. In the case of extract extracted with a mixed solvent of alcohol and n-heptane, the content of ginkgolic acid may be used as a main ingredient, for example, as an insecticide or a repellent, and is suitable for use in outdoor spraying applied directly to plants.
또한, 본 발명에 따른 은행잎 추출물은 집진드기 살비제, 의류의 부패방지 및 항균성 코팅제, 종이류의 장기보관용 첨가제 등으로 사용할 수 있으며, 특히 천연 성분으로 인체에 유해하지 아니하므로 주방용품, 가전제품, 의류, 섬유제품, 식품세척 등을 포함하여 각종 생활용품의 세척이나 항균성 부여를 위해 사용할 수 있고, 화초 등 원예작물과 농작물, 과수작물, 온실, 화분, 토양개량용 등으로 다양하게 적용할 수 있다.In addition, ginkgo biloba extract according to the present invention can be used as a mite acaricide, anti-corrosive and antimicrobial coating of clothing, additives for long-term storage of paper, etc. In particular, because it is not harmful to the human body as a natural ingredient, kitchen appliances, home appliances, clothing It can be used for washing various kinds of household goods or giving antimicrobial properties, including textile products, food washing, etc., and can be applied to various horticultural crops such as flowers, crops, fruit crops, greenhouses, pots, and soil improvement.
본 발명에 따라 제조된 추출물을 항균, 살충용 유효성분으로 사용하는 경우 대개는 원액을 물에 희석하여 사용할 수 있으며, 경우에 따라서는 다른 천연 항균 또는 살충 성분들과 적절히 혼합한 형태로도 사용할 수 있다. 그리고, 통상적으로 사용되는 비료나 세정제 등과 같은 다른 유효성분이나 부형제 등 첨가제와 혼합하여 사용할 수 있고, 색소, 계면활성제, 방부제 등을 첨가하여 사용할 수 있으며, 분말화 하거나 펠릿화, 그래뉼화, 정제화하는 방법으로 제형을 변경하여 통상의 방법으로 포장하여 제품화하고 일반적인 방법으로 널리 사용할 수가 있다.When the extract prepared according to the present invention is used as an active ingredient for antibacterial and insecticide, it is usually possible to dilute the stock solution in water, and in some cases, it may also be used in a form in which it is properly mixed with other natural antibacterial or insecticidal ingredients. have. In addition, it can be used in combination with other active ingredients such as fertilizers, detergents, etc. commonly used additives such as excipients, and can be used by adding pigments, surfactants, preservatives, etc., powdered or pelletized, granulated, tableted The formulation can be changed by the method, packaged in the usual way, and commercialized, and widely used in the general way.
일반적인 적용 방법은 전형적으로는 물에 녹이거나 희석하여 사용하는 방법과 분무하는 방법 등과 같이 그 형태와 적용목적, 적용분야에 따라 다양한 방법을 사용할 수 있다.Typical application methods are typically a variety of methods depending on the type, application and application, such as dissolved or diluted in water and sprayed.
그 적용 량은 사용 목적과 적용형태, 적용부위, 적용대상물품 등에 따라 적절히 사용할 수 있는 것이며, 본 발명의 추출물은 천연성분이고 그 제품의 특성상 적용목적을 달성할 수 있다면 다양한 량으로 사용할 수 있으므로, 특별히 적용량이나 적용 방법을 한정하는 것은 별 의미가 없다.The application amount may be appropriately used according to the purpose of use, application form, application area, target object, and the like, and the extract of the present invention may be used in various amounts as long as the extract of the present invention is a natural ingredient and the purpose of application may be achieved due to the characteristics of the product. It is not particularly meaningful to limit the application amount or application method.
이와 같이, 은행잎으로부터 항균, 살충 및 해충의 기피효과를 갖는 물질을 규명하기 위하여 은행잎으로부터 활성성분을 분리·정제하는 방법과 그 적용 용도 등을 좀더 구체적으로 설명하기 위하여 실시예로 설명하면 다음과 같다.As described above, the method for separating and purifying the active ingredient from the ginkgo biloba leaves, and its application, etc. in order to identify substances having antibacterial, insecticidal and pest repelling effects from the ginkgo biloba will be described in detail as follows. .
본 발명의 실시예는 본 발명을 설명하기 위해 예시한 것이지 본 발명은 한정하는 것은 아니다.The embodiments of the present invention are illustrated to illustrate the present invention, but the present invention is not limited.
실시예 1 : 증류수 추출Example 1 Extraction of Distilled Water
생 은행잎과 증류수(물)를 1 : 0.5의 중량비로 혼합하여 믹서로 갈아 스럿지(sludge) 상태로 만든 후 원심분리기로 분리하여 고형성분과 액상성분을 분리하였다. 이렇게 제조 분리된 용액의 주요 성분을 분석한 결과 추출용액 100g당 플라본글리코사이드류 2.3g, 테르펜락톤류 0.2g, 징콜릭애시드류 3.5g 등이 포함되어 있음을 확인 할 수 있었다.Fresh ginkgo biloba leaves and distilled water (water) were mixed at a weight ratio of 1: 0.5, ground in a mixer, sludged, and separated by a centrifuge to separate solid and liquid components. As a result of analyzing the main components of the prepared separated solution, it was confirmed that flavone glycosides 2.3g, terpene lactones 0.2g, ginkgolic acid 3.5g and the like per 100g of the extraction solution.
또한, 이러한 동일 실험을 반복하여 실시해 본 결과, 은행잎의 수집지역에 따라 활성물질의 농도는 차이를 보이는 것으로 나타났다.In addition, as a result of repeating the same experiment, it was found that the concentration of the active substance was different according to the collection area of the ginkgo leaves.
실시예 2 : 증류수 중탕 추출Example 2 Extraction of Distilled Water
생 은행잎중 낙엽상태의 노란 은행잎과 건조은행잎으로부터 수용성 활성물질을 추출하기 위한 방법으로 사용한다.It is used as a method to extract water-soluble active substances from deciduous yellow ginkgo biloba leaves and dried ginkgo biloba leaves.
은행잎(생은행잎, 건조 은행잎)을 잘게 썰고 물과 1: 0.5로 혼합하여 60oC ~ 100oC 온도에서 20분간 중탕하여 갈녹색의 용액을 추출물로 얻었다.Ginkgo biloba leaves (fresh ginkgo biloba leaves, dried ginkgo biloba) were finely chopped and mixed with water at 1: 0.5, and then bathed at 60 o C to 100 o C for 20 minutes to obtain a brownish green solution as an extract.
상기에서 얻은 추출물을 분석한 결과, 추출용액 100g 당 플라본글리코사이드류 2.8g, 테르펜 락톤류 0.4g, 징콜릭애시드류 13.5g을 포함하는 것으로 확인되었다. 이 결과로부터 상기 실시예 1의 경우에 비하여 중탕한 경우 추출용액 중에 징콜릭애시드의 농도가 증가하였다.As a result of analysis of the extract obtained above, it was confirmed that the fluorine glycosides 2.8g, terpene lactone 0.4g, ginkgolic acid 13.5g per 100g extract solution. From this result, the concentration of ginkgolic acid in the extraction solution was increased in the hot water compared to the case of Example 1.
실시예 3 : 메탄올과 n-헵탄 혼합용매 추출Example 3 Methanol and n-heptane Mixed Solvent Extraction
생은행잎으로부터 수용성 및 지용성 성분을 보다 용이하게 추출하기 위하여 메탄올과 n-헵탄을 1:1 로 혼합한 후 이 용액에 상기 실시예 1에서 원심분리하고 남은 고형분(함수율 65%)을 혼합한 후 정치하면 메탄올과 n-헵탄층이 분리되는데 비중차이에 의하여 밑층에는 메탄올 성분 윗층에는 n-헵탄 성분의 층이 형성되며 이를 각각 분리한 후 0.9 X 103Pa에서 감압증류하여 메탄올과 n-헵탄을 각각 회수하였다.In order to more easily extract the water-soluble and fat-soluble components from the fresh ginkgo biloba, methanol and n-heptane were mixed in a 1: 1 ratio, and the solution was centrifuged in Example 1, and the remaining solids (65% water content) were mixed. Methanol and n-heptane layer are separated, and due to the difference in specific gravity, a layer of n-heptane layer is formed on the upper layer of methanol component and separated from each other, followed by distillation under reduced pressure at 0.9 X 10 3 Pa to form methanol and n-heptane, respectively. Recovered.
상기에서 얻은 각 층의 추출물에 대하여 유효성분의 함량을 측정한 결과 메탄올층의 경우는 추출물 100g 당 플라본글리코사이드류 3.8g, 테르펜락톤류 0.75g, 징콜릭애시드류 5.3g으로 분석되었으며, n-헵탄층의 경우는 추출물 100g 당 플라본글리코사이드류 0.2g, 테르펜락톤류 0.08g, 징콜릭애시드류 25.3g 등으로 확인되었다.As a result of measuring the content of the active ingredient with respect to the extract of each layer obtained above, the methanol layer was analyzed to be 3.8g of flavone glycosides, 0.75g of terpene lactones and 5.3g of zinc acid per 100g of extract, and n- In the case of the heptane layer, 0.2 g of flavone glycosides, 0.08 g of terpene lactones, 25.3 g of ginkgolic acid, etc., per 100 g of extract were identified.
참고예 : 은행잎 추출물의 성분 분석Reference Example: Component Analysis of Ginkgo Leaf Extract
상기와 같은 실시예에서 얻은 유효성분의 특성과 성분의 구체적인 내용을 확인하고 분석하기 위하여, 은행잎 성분을 크게 추출물과 최종 고형물로 분류하여 HPLC-MASS로 분석하여 성분의 함량과 구조를 분석하였다.In order to check and analyze the specific contents and characteristics of the active ingredients obtained in the above examples, ginkgo biloba was divided into extracts and final solids and analyzed by HPLC-MASS to analyze the content and structure of the ingredients.
분석한 결과, 최종 고형물은 셀루로오즈 성분과 노나코산- 10-올(지놀)[ nonacosan-10-ol(ginnol)]성분과 노나코산-10-온(nonacosan-10-one)이 주성분으로 밝혀졌다. 또한, 알파토코페롤(α-tocopherol)도 성분이 확인되었다. 특히, 추출물 중에 함유되어있는 성분들 중에서 향균성 및 살충 성분이 있는 물질은 징콜릭애시드(ginkgolic acid), 플라본글리코사이드(flavone glycoside) 및 테르펜락톤(terpene lactone)류 이며 이에 대한 자세한 분석결과는 다음과 같았다.The final solids were composed of cellulose, nonacosan-10-ol (ginnol) and nonacosan-10-one. Turned out to be. In addition, alpha-tocopherol was also identified as a component. Particularly, among the components contained in the extracts, antibacterial and insecticidal substances are ginkgolic acid, flavone glycoside and terpene lactones. It was like
징콜릭애시드류로서는 3-알킬페놀류와 2-카복실-3-알킬-페놀로 구성되며, 여기서 알킬기는 탄소수가 13개, 15개(이중결합 1개 포함), 17개(이중결합 1개 포함)인 물질이 각각 14%, 42% 44%로 구성되어 있는 것으로 확인되었다.Ginkgolic acids include 3-alkylphenols and 2-carboxy-3-alkyl-phenols, wherein the alkyl group has 13 carbon atoms, 15 carbon atoms (including 1 double bond) and 17 carbon atoms (including 1 double bond). It was confirmed that the phosphorus material was composed of 14%, 42% and 44%, respectively.
또한, 플라본글리코사이드류로서는Moreover, as flavone glycosides
1) Sciadopitysin1) Sciadopitysin
2) Ginkgetin2) Ginkgetin
3) Isoginkgetin3) Isoginkgetin
4) Bilobetin4) Bilobetin
5) Amentoflavone5) Amentoflavone
6) Kaempferol 3-O-[2",6"-α-L-dirhamnopyranosyl-β-D-glucopranoside6) Kaempferol 3-O- [2 ", 6" -α-L-dirhamnopyranosyl-β-D-glucopranoside
7) Kaempferol 3-O-rutinoside7) Kaempferol 3-O-rutinoside
8) Quercetin 3-O-6"'-O-p-cuomarol-β-D-glucopy-ranosyl(1->2)-α-L- rhamnopyranoside8) Quercetin 3-O-6 "'-O-p-cuomarol-β-D-glucopy-ranosyl (1-> 2) -α-L-rhamnopyranoside
9) Isorhamenetin 3-Orutioside9) Isorhamenetin 3-Orutioside
10) Rutin10) Rutin
11) Larictrin-3-O-rutinoside11) Larictrin-3-O-rutinoside
12) Kaempferol 3-O-6"'-O-p-coumarol-β-D-glucopyranosyl(1-2)-α-L- rhamnopyranoside12) Kaempferol 3-O-6 "'-O-p-coumarol-β-D-glucopyranosyl (1-2) -α-L- rhamnopyranoside
등의 성분을 포함하는 것으로 확인되었다.It was confirmed to contain components, such as these.
그리고, 테르펜락톤류로서는 빌로발라이드(Bilobalide) 와 징콜라이드(Ginkgolide)가 주성분이며, 각 추출방법에 따라 총 활성물질의 0.1 ~ 7 중량%의 성분비를 갖는 것으로 확인되었다.In addition, as terpene lactones, bilobalide and ginkgolide were the main components, and it was confirmed that each of the extraction methods had a component ratio of 0.1 to 7% by weight of the total active substance.
실험예 1 : 항균력 실험Experimental Example 1: Antimicrobial Activity
본 실험에서 은행잎 추출물 의한 살균실험에서 사용된 균주는 박테리아Escherichia coli(AB1157)이고, 곰팡이로Aspergillus oryzae var. oryzae(KCTC 6095)이며 병충해 유발균으로는 오이 및 토마토의 시드름병의 원인균인Pseudomonas corrugata(KACC10141),Pseudomonas syringae(KACC10396)와Ralstonia solanacearum(KACC 10475), 탄저병의 원인균인Glomerella cingulata,상추역병균인Phytophthora drechsleri이었다.Escherichia coli(AB1157)배양은 배양은 LB Medium (pH 7.0, 37℃)에서 24시간 진탕 배양하였으며, 이에 대한 자세한Escherichia coli(AB1157)배양액 조성은 다음 표 1에 정리하였다. 세포농도는 50 mM의 포스페이트포타슘염 완충용액(pH 7.0)을 사용하여 연속희석방법으로 결정하였다. 활성물질 투여 후 살아남은 균체는 적당히 희석하여 영양한천배지에 100 ㎕씩도말하고 24시간 배양한 후 계수하였다.A. oryzae는 감자덱스트로스 (Difco)에 한천 (1.5 %, Junsei)을 넣은 사면배지에 접종한 후 4일간 30 ℃에서 배양하였고, 균주로부터 코니디아(conidia)가 생성되었을 때 트윈 80 (0.02 %, Junsei)을 3 ㎖넣고 휘저어(vortex) 코니디아를 떼어내었으며, 코니디아의 농도는 헤마시토메타(hemacytometer)를 이용하여 107conidia/㎖임을 확인하고 실험에 적용하였다. 살균반응으로 얻은 분획들은 연속 희석하여 곰팡이의 성장을 조절할 수 있는 0.1 % oxgall을 넣은 PDA 고체배지에 100 ㎕씩 각각 도말하여 반응중에 살아남은 균체수를 확인하였다. 그외 세균의 배양은 모든 균에 대하여 King's B Medium (pH 7.2, 27℃)에서 24hr 진탕 배양하였다. 균주의 성장에 이용되는 King's B 배지의 배양액 조성은 다음 표 2와 같다.In this experiment, the strain used in the sterilization experiment with ginkgo biloba extract was the bacterium Escherichia coli (AB1157) . Aspergillus oryzae var. oryzae (KCTC 6095), and the pest causing bacteria include Pseudomonas corrugata (KACC10141), Pseudomonas syringae (KACC10396), Ralstonia solanacearum (KACC 10475), and the causative agent of anthrax, Glomerella cingulata. Phytophthora was drechsleri . Escherichia coli (AB1157) culture was cultured for 24 hours shaking in LB Medium (pH 7.0, 37 ℃), detailed Escherichia coli (AB1157) culture composition is summarized in Table 1 below. Cell concentration was determined by continuous dilution method using 50 mM phosphate potassium salt buffer (pH 7.0). The cells survived after administration of the active substance were diluted properly, smeared 100 μl in nutrient agar medium and incubated for 24 hours before counting. A. oryzae was inoculated in potato dextrose (Difco) agar plate with agar (1.5%, Junsei) and incubated at 30 ° C for 4 days. When conidia was produced from the strain, Tween 80 (0.02% , 3 mL of Junsei) was added and vortex condydia was removed, and the concentration of condydia was confirmed to be 10 7 conidia / ml using a hemacytometer. The fractions obtained by the sterilization reaction were serially diluted to 100 μl each of the PDA solid medium containing 0.1% oxgall to control the growth of the fungus to determine the number of cells survived in the reaction. All other bacteria were cultured in King's B Medium (pH 7.2, 27 ° C) for 24hr shaking for all bacteria. The culture composition of King's B medium used for growth of the strain is shown in Table 2 below.
상기와 같은 조건에서 상기 실시예 1, 2, 3에서 각각 얻은 은행잎 추출물과대조약제로서 앰피실린에 대하여 미생물에 대한 항균력을 비교 실험을 하였으며, 그 결과는 다음 표 3에 나타내었다.Under the above conditions, the antimicrobial activity against the microorganisms was compared to ampicillin as the ginkgo biloba extract obtained in Examples 1, 2, and 3 as a control agent, and the results are shown in Table 3 below.
실험예 2 : 살충력 실험Experimental Example 2: Insecticidal Test
상기 실시예 1, 2, 3으로부터 얻어진 은행잎 추출물에 대한 주요 해충의 살충효과를 검정하기 위해 점박이응애(Tetranychus urticae), 복숭아혹진딧물(Myzus persicae)을 대상으로 살충력 실험을 행하였다. 점박이응애의 약제독성시험 방법으로 leaf disk법을 사용하였고 암컷성충의 살비효과는 강낭콩엽을 2×2cm로 잘라 물에 적신 탈지면이 깔린 샤레에 놓고 그위에 점박이응애 암컷성충을 30마리씩 가는 붓으로 옮겨 놓았다. 30분 정도 정착을 시킨 다음 추출물별로 샤레에서 25cm 거리에서 hand spray로 엽편이 충분히 적셔질 정도로 5초동안 스프레이 하고 음건시켰다. 처리한 샤레는 상온에 보관하여 24, 72시간 후에 살비율을 조사하였다. 생사판별은 해부현미경하에서 붓으로 충체를 접촉하여 몸길이 정도를 이동하지 못하는 개체는 죽은 것으로 간주하였다. 복숭아혹진딧물은 유묘검정법을 사용하였는데 고추 묘에 미리 진딧물을 접종하여 정착된 유묘 잎의 진딧물수를 조사한 후 3반복으로추출물을 hand spray로 살포하고 24, 72 시간후 살충율을 조사하였다. 이와 같은 실험 결과를 다음 표 4에 정리하였다. 먼저 각 추출물에 가장 효과를 나타낸 것은 실시예 1의 추출물을 적용한 점박이응애로서 24시간 이내에 최고 97%, 72 시간이내에 최고 100%의 효과를 보여주어 농약으로서의 우수한 살충력을 나타내는 것으로 확인되었다. 상기 실시예 3의 추출물에 대해서는 복숭아혹진딧물이 가장 우수하였으며 72시간 이후에 99.6%의 살충율 효과를 나타내었다.In order to test the insecticidal effect of the main pests on the ginkgo biloba extract obtained in Examples 1, 2, 3, insecticidal experiments were conducted on Tetranychus urticae and Peach aphids ( Myzus persicae ). The leaf disk method was used as a test for the toxicity of spotted mite, and the fertilizing effect of the female adult was cut into 2 × 2cm and placed in a share moistened with water, and the spotted mite female adult was transferred to a brush with 30 grains on it. Let go. After settling for about 30 minutes, each extract was sprayed and dried for 5 seconds so that the leaves were sufficiently wetted by hand spray at a distance of 25 cm from the curry. The treated curry was stored at room temperature and examined for the rate of fattening after 24 and 72 hours. Life and death discrimination was considered to be dead for individuals who could not move their body length by touching the tooth with a brush under a dissecting microscope. The peach aphid was used as a seedling assay, and the seedlings were inoculated with aphids in advance, and the number of aphids on the seedlings of the seedlings was settled in three replicates. The experimental results are summarized in Table 4 below. First, it was confirmed that the most effective effect on each extract was the spot mite applying the extract of Example 1, showing an effect of up to 97% within 24 hours and up to 100% within 72 hours, showing excellent insecticidal properties as a pesticide. For the extract of Example 3, the peach aphid was the best and showed an insecticide effect of 99.6% after 72 hours.
실험예 3 : 해충 기피제(repellent) 효과 실험Experimental Example 3: Pest repellent effect experiment
상기 실시예 1, 2, 3에서 각각 얻은 은행잎 추출물에 대한 주요 해충의 기피효과를 검정하기 위해 점박이응애(Tetranychus urticae), 담배거세미나방(Spodoptera litura), 배추좀나방(Plutella xylostella), 복숭아혹진딧물(Myzus persicae)을 대상으로 하였다.To test the repellent effect of the main pests on the ginkgo biloba extract obtained in Examples 1, 2, and 3 respectively, Tetranychus urticae , Spodoptera litura , Plutella xylostella , Peach hump Aphids ( Myzus persicae ) were targeted.
점박이응애는 강낭콩엽을 2×2cm로 잘라 각 추출법에 따라 제조한 추출물을 hand spray로 살포하고 각 충수를 30마리 암컷성충을 30마리씩 가는 붓으로 옮기고 5cm 거리 샤레위에 상온에서 추출물과 함께 격리시킨 후 24, 72시간 후에 기피율율을 조사하였다.Spotted mite is cut into 2 × 2cm of kidney beans and sprayed with hand spray to extract the extract prepared according to each extraction method, and each appendix is transferred to 30 adult females with a brush of 30 females. The evasion rate was examined after 24 and 72 hours.
담배거세미나방의 기피율은 배추가 식재된 포트에 시험해충(2∼3령 유충)을 10마리씩 접종하여 정착시킨 후 추출물을 hand spray로 배추에 살포하여 24, 72시간에 배추위의 충수를 조사하였으며 3반복 시험하였다.The evacuation rate of tobacco castor moth was inoculated with 10 test insects (2 ~ 3 year old larvae) into the pot where cabbages were planted. And three repeated tests.
복숭아혹진딧물은 유묘검정법을 사용하였는데 고추 묘에 미리 진딧물을 접종하여 정착된 유묘 잎의 진딧물수를 조사한 후 3반복으로 추출물을 hand spray로 살포하고 24, 72 시간후 기피율을 조사하였다.The peach aphid was used as a seedling assay, and the seedlings were inoculated with aphids in advance to check the aphid number of the seedling leaves. The extract was sprayed with hand spray three times and then evacuated after 24 and 72 hours.
이와 같은 모든 실험에서 해충은 먹이를 기피하여 먹이 위의 해충은 없었다. 따라서 은행잎 추출물은 추출법에 관계없이 기피효과를 갖는다는 것을 확인할 수 있었다.In all these experiments, the pests avoided the food and there were no pests on the food. Therefore, the ginkgo leaf extract was found to have a repellent effect regardless of the extraction method.
상술한 바와 같이, 본 발명은 종래와는 달리 특정 조성의 유효성분을 함유하는 형태의 은행잎 추출물로서, 플라본글리코사이드류, 테르펜락톤류 및 징코릭 애시드류를 소정량 함유하는 성분으로 추출한 추출물을 유효성분으로 하는 각종 제품을 상품화하여 항균제, 살균제 또는 해충기피제 등과 같은 농약으로 사용하는 경우인체에 전혀 유해하지 아니하고, 적절한 형태로 하여 수경재배용 양액, 과일 보관용제, 집진드기 살비제, 의류의 부패방지 및 항균성 코팅제, 종이류의 장기보관용 첨가제 등으로도 효과적으로 사용할 수 있는 효과가 있다.As described above, the present invention, unlike the prior art as a ginkgo leaf extract containing an active ingredient of a specific composition, the extract extracted with a component containing a predetermined amount of flavone glycosides, terpene lactones and ginkgolic acids effective When commercializing various products with ingredients and using them as pesticides such as antibacterial agents, fungicides or pest repellents, they are not harmful to the human body at all and are in proper form for hydroponic nutrient solution, fruit storage agent, dust mite acaricide, clothing anti-corruption and antibacterial effect. There is an effect that can be effectively used as a coating agent, additives for long-term storage of papers and the like.
Claims (13)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2001-0010760A KR100415107B1 (en) | 2001-03-02 | 2001-03-02 | Ginko biloba extract and preparing methods and use thereof |
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| KR10-2001-0010760A KR100415107B1 (en) | 2001-03-02 | 2001-03-02 | Ginko biloba extract and preparing methods and use thereof |
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| KR100415107B1 true KR100415107B1 (en) | 2004-01-13 |
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Families Citing this family (17)
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| KR20030005671A (en) * | 2001-07-10 | 2003-01-23 | 김병규 | A fruit of protection an envelope manufacture method |
| KR100543279B1 (en) * | 2002-08-07 | 2006-01-20 | 정재윤 | Extraction ? Application of Antibacterial ? Antifungal Agents for Textile |
| KR100579710B1 (en) * | 2003-09-16 | 2006-05-15 | 학교법인조선대학교 | Shampoo composition for treating dandruff and alopecia containing compounds obtained from ginkgo biloba l. leaves |
| KR100583634B1 (en) * | 2004-04-30 | 2006-05-26 | (주)동명에릭스 | Composition for Vermin Control |
| KR100644750B1 (en) * | 2004-05-06 | 2006-11-14 | 주식회사 다우존팜 | Method for producing insect natual extract |
| ATE528058T1 (en) * | 2004-07-29 | 2011-10-15 | Mitsubishi Paper Mills Ltd | USING AN AIR FILTER WITH GINKGO EXTRACT |
| KR100783152B1 (en) * | 2005-05-20 | 2007-12-07 | 학교법인 한림대학교 | Antibacterial composition containing ginko extract |
| CN100462006C (en) * | 2006-02-21 | 2009-02-18 | 复旦大学 | Use of ginkgoic acid in preparation of biological pesticide for killing Oncomelania snail and preventing schistosomiasis |
| EP2087900A1 (en) * | 2008-02-07 | 2009-08-12 | Velleja Research SRL | Ginkgo biloba extract with a standardised ginkgo flavone glycosides content deprived of the paf-antogonist terpenic fraction, and compostions containing it, for the prevention and treatment of circulatory, cognitive, geriatric and sensory disorders |
| CN103299998B (en) * | 2012-03-15 | 2015-02-04 | 北京鑫洋水产高新技术有限公司 | Application of ginkgolic acid in killing of blue-green algae |
| KR101441614B1 (en) * | 2012-09-26 | 2014-09-17 | 박화목 | Insecticide or compost composition comprising the leaves of Ginkgo biloba by water aging |
| KR101261359B1 (en) * | 2012-11-09 | 2013-05-07 | (주)크린바이오 | A composition of a selective termite repellent containing natural vegetable material |
| KR101527513B1 (en) * | 2014-02-05 | 2015-06-09 | 나눔협동조합 | Air purifier composition contained pine needle oil and method thereof |
| CN109793233A (en) * | 2018-07-25 | 2019-05-24 | 上海药辰生物科技有限公司 | A kind of deep working method of ginkgo nut endosperm |
| KR102202303B1 (en) | 2020-07-29 | 2021-01-13 | 이준홍 | Insecticide composition using ginkgo leaves and method for manufacturing the same |
| RU2767255C1 (en) * | 2021-02-02 | 2022-03-17 | федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Method for producing a dry extract from ginkgo biloba leaves |
| KR102644589B1 (en) * | 2021-11-09 | 2024-03-06 | 구원혁 | Natural composition for insect repellent comprising ginkgo biloba extract |
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| KR20020070598A (en) | 2002-09-10 |
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