CN1357229A - Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent - Google Patents
Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent Download PDFInfo
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Abstract
Artificially cultivated elsholtzia herb is extracted to obtian thymol and carvacrol, which is further produced into elsholtzia herb essence containing thymol in 9.5% and elsholtzia herb essence oil containing thymol in 59% and carvacrol in 0.13%. The elsholtzia herb essence and elsholtzia herb essence oil are compounded with 500-3000 times water in water solution for preventing and eliminating plant pest and disease and inhibiting the growth of bacteria and fungi. The present invention has several effective components and no resistance produced and prdouces no harm to human and pollution to environment.
Description
Application of the natural agent for preventing and eliminating Mosla chinensis of plant pest of the present invention and production thereof and processing relate to the invention of protecting crops, belong to the agricultural plant protection field.
Mosla chinensis claims " cavalerie mosla herb ", " magnificent shepherd's purse is peaceful " formal name used at school again, and (latin name Mosla ChinensisMaxim) is wild in the meadow, hillside, and the each province, the southeast on the south 39 ° of China's north latitude has.Development test shows, the isomer carvacrol (Carvacrol) of Thymol that contains in the herb of Mosla chinensis overground part (Thymal) and Thymol all has significant inhibitory effect to most bacteriums, fungi and virus, and stronger expelling parasite, insecticidal action are also arranged simultaneously.Mosla chinensis is used for operation as traditional traditional Chinese medicine a long time ago, the skin degerming during injection, treatment flu, influenza, infectious diseases etc.Application at aspects such as agricultural plant protections, beyond example still both at home and abroad.Present control to plant pest, as the cucumber downy mildew (Leveillula taurica) of relatively representative type phytopathy (Sphaerothecafuligina), the control of strawberry anthracnose (Colletotri fragariae) etc. all is to use Sandofan (Switzerland's product), Ke Lu (U.S.'s product), peace gram (U.S.'s products), violent zinc (homemade), thiophanate methyl chemosynthesis bactericide such as (Japan produce), to the control use Rogor of aphid.
Chemical pesticide, none is harmful to environment for human survival, is polluting atmosphere, territory, underground water, and that be injured maximum is the operating personnel of applying pesticides, sucks a large amount of agricultural chemicals, severe damaging body health in their body.
The objective of the invention is to provide a kind of can the substituted chemistry synthetic pesticide, contributive control to plant pest is long-acting natural plant pest agent for preventing and eliminating Mosla chinensis to human survival to natural environment.
When being used to prevent and treat plant pest, the natural agent for preventing and eliminating Mosla chinensis of plant pest of the present invention Mosla chinensis need be carried out artificial cultivation to obtain the Mosla chinensis herb and the effective component extracting of stable a large amount of cheapness, be Thymol and carvacrol, and make elscholtiza essence that contains Thymol or the elscholtiza essential oil that contains Thymol and carvacrol, this elscholtiza essence or Mosla chinensis essential oil and 500 times or 3000 times of water are mixed with the aqueous solution, can be used for preventing and treating cucumber downy mildew, strawberry anthracnose, tomato early blight, the tomato late blight, the eggplant cotton disease, the green pepper eqpidemic disease, tomato, capsicum, eggplant, cucumber, the gray mold of custard squash and melon downy mildew, powdery mildew all obtains tangible control efficiency.For example, when the cucumber downy mildew of difficult control in control above-mentioned fruit and vegetable all diseases, for the every strain of cucumber from bottom to top blade spray by strain all under the morbid state of adularescent spot, every 15 days, spray again, sprouting (axillalry bud) conservation rate is reached more than 80%; Also can be used for preventing and treating the insect pest of fruit aphid, to the fruit tree uniformly of taking place and distributing, spray by strain, every 15 days, spray again, the result is that aphid can not regenerate, and fruit tree spray, tender leaf do not come to harm yet.Suppressing the bacterium class, the yeast class, during the growing of Mycophyta, even fresh bacterial strain through continuous 3 times above successive transfer culture, the flower of wild or tame Mosla chinensis, leaf or stem branch are under the various dose effect of the smart aqueous solution of the raw material elscholtiza of making, to suppressing yellow staphylococcus, the withered grass bacterium, colon bacillus, upright squama germ, Bacteria erwinia, yeast, black chrysanthemum is mould, the tinea alba bacterium, the mould germ of grey, the white root rot bacterium, Candida aldicans IFO 1594 (yeast class), Aeromonas salmonicida Sudsp-salmonicida 12659 (bacterium class), Fusarium oxysporum f.sp lycopersici IFO 31213 (Mycophytas) grows effectively, wherein, flower with Mosla chinensis, leaf is under the various dose effect of the smart aqueous solution of the raw material elscholtiza of making, to suppressing pine root fungus IFO7707, pine root fungus IFO9425, pine root fungus IFO31881, the seedling blight bacterium, epidemic disease bacterium, paddy rice rot bacterium, growing effectively of bavin brown root rot bacterium, the flower that also has tame Mosla chinensis, leaf is under the various dose effect of the smart aqueous solution of the raw material elscholtiza of making, to suppressing the sclerotium germ, growing effectively of pneumobacillus.
The artificial cultivation method of the natural agent for preventing and eliminating Mosla chinensis of plant pest of the present invention is that the Mosla chinensis seed is placed-5 ℃--under 10 ℃, after 10 days-30 days, bury with wet sandy soil, after 20 days, seeding and seedling raising, germination rate are about 50%.
By Mosla chinensis prepare the elscholtiza essence be the method for Mosla chinensis extract be Mosla chinensis acrial part herb is carried out drying, pulverizes after, add the methyl alcohol or the ethanol that are equivalent to 10 times of raw materials, stir, after filtering extract, heating, recovered under reduced pressure methyl alcohol or ethanol, obtain extract and residue for the first time, repeat for the first time same immersion and extract toward reinject methyl alcohol or ethanol of residue, the extract mixture of twice gained is lumped together heating, concentrating under reduced pressure, obtain to contain the elscholtiza essence of 9.0%-10.0% Thymol and about 50% water.
Preparing the elscholtiza essential oil by Mosla chinensis is that the method for volatile oil is to steam the slide method with steam, Mosla chinensis is contained volatile oil steam with steam, with water-oil separating, obtains to contain the elscholtiza essential oil of 55.0%-60.0% Thymol and 0.10%-0.15% carvacrol.
Smart from the elscholtiza that contains Thymol of natural labiate Mosla chinensis extraction and contain Thymol and carvacrol elscholtiza essential oil (volatile oil) is applied to crop and comes prevention and elimination of disease and pests, and this goes back beyond example at home and abroad.Elscholtiza essence or elscholtiza essential oil (volatile oil) to its pathogeny of control of phytopathy to as if wide spectrum, be rare can be to bacterium, fungi effective agent for preventing and eliminating simultaneously, because the present invention contains and is not single active ingredient, various pathogenies are difficult to produce resistance, are again a kind of long-acting plant pest agent for preventing and eliminating therefore.Having that the present invention uses is antibiotic, the Thymol and the carvacrol of sterilization, insecticidal action are not chemicals; it is the natural materials that is present in the labiate Mosla chinensis; pollution-free to large natural environment, so the environmental protection that pay attention to day by day is got up is of great importance.
The invention will be further described with embodiment below:
Embodiment 1 cucumber downy mildew control example
The multiparasitism of cucumber downy mildew fungal component is the pathogeny of cucumber downy mildew in mellon plant and plant of Solanaceae.
(1) test site and time: Dalian, on August 22nd, 1999, September 5, carry out 2 times and use test;
(2) selection of experimental enviroment: the cucumber outdoor cropping, one period of cucumber downy mildew morbidity Sheng phase on August, the middle ten days.Generally fall ill in the nursery of implementing test nearly hundred strain cucumber altogether, complete stool is blade adularescent spot all from bottom to top.For 3 row totally 12 strains of examination cucumber North and South direction, wherein use the smart 4 strain A1-4 of elscholtiza, check plot Shi Kelu 4 strain B1-4, blank 4 strain C1-4;
(3) test method and result: will do the record of sprouting number scale for three sections of examination 12 strain cucumber branch upper, middle and lower before using test on August 22, the height on ground to 1 meter is decided to be hypomere, 1 meter-1.6 meters are decided to be the stage casing, 1.6 be decided to be epimere more than the rice, A1-4, B1-4, C1-4 count investigation records for examination 12 strain sproutings and see Table 1.
Table 1 supplied the sprouting number (axillalry bud) of examination 12 strains on August 22
| Strain | Epimere stage casing hypomere | Add up to | Amount to | Conservation rate % |
| ????A1 ????A2 ????A3 ????A4 | ????4??????5??????4 ????3??????5??????3 ????5??????4??????5 ????4??????5??????4 | ????13 ????11 ????14 ????13 | ???51 | ????100 |
| ????B1 ????B2 ????B3 ????B4 | ????4??????3??????4 ????5??????4??????4 ????3??????4??????4 ????5??????4??????5 | ????11 ????13 ????11 ????14 | ???49 | ????100 |
| ????C1 ????C2 ????C3 ????C4 | ????5??????4??????4 ????4??????4??????4 ????5??????4??????3 ????4??????3??????4 | ????13 ????12 ????12 ????11 | ???48 | ????100 |
August 22,1 liter of the every strain of 500 times of aqueous solution of using the elscholtiza essence that contains 9.6% Thymol to A1-4 is used the U.S. to B1-4 and is produced gram and reveal 1 liter of 1000 times of every strain of the aqueous solution, does not use any medicine to C1-4 and makes blank.After on September 5 in i.e. 2 weeks, do a sprouting and count investigation records, the results are shown in Table 2.
September 5 was carried out sprouting and counts after the investigation records, and repeating for the first time is the test method on August 22, carried out the test second time.September 18, the sprouting that tries again was counted investigation records, saw Table 3.
Above-mentioned result of the test can confirm by the elscholtiza essence that Mosla chinensis is extracted cucumber downy mildew is had tangible control efficiency, and the control of plant disease is had practicality.
Table 2 sprouting on September 5 is counted survey data
| Strain | Epimere stage casing hypomere | Add up to | Amount to | Conservation rate % |
| ????A1 ????A2 ????A3 ????A4 | ????6????????5???????4 ????5????????6???????3 ????5????????5???????5 ????5????????5???????4 | ????15 ????14 ????15 ????14 | ???58 | ????113.7 |
| ????B1 ????B2 ????B3 ????B4 | ????4????????3???????2 ????4????????4???????3 ????3????????3???????4 ????4????????3???????3 | ????9 ????11 ????10 ????10 | ???40 | ????81.6 |
| ????C1 ????C2 ????C3 ????C4 | Complete stool is withered | |||
Table 3 sprouting on September 18 is counted investigation result
| Strain | Epimere stage casing hypomere | Add up to | Amount to | Conservation rate % |
| ????A1 ????A2 ????A3 ????A4 | ?????5?????4??????3 ?????4?????3??????3 ?????5?????3??????3 ?????4?????4??????3 | ????12 ????10 ????11 ????11 | ????44 | ????86.3 |
| ????B1 ????B2 ????B3 ????B4 | ?????0?????1??????1 ?????2?????1??????1 ?????1?????1??????0 ?????1?????1??????0 | ????2 ????4 ????2 ????2 | ????10 | ????20.4 |
| ????C1 ????C2 ????C3 ????C4 | All whole strain comes off from frame | |||
Embodiment 2 strawberry anthracnose control example
(1) experimental field: Japan Fukushima county, test period: 17 days-December 11 November in 1999;
(2) test method: in the greenhouse, will cultivate in the alms bowl of 9 centimeters of diameters for examination strawberry cultivars " women peak ".A, establish 3 test regions, a district 10 strains.The method of control, sprinkling date, number of times, blanking time see Table 1.The inoculation of B, anthracnose: (spraying after 5 days) will adjust November 22 5 * 10
4Conidial suspension of cfu/ml concentration sprays 4ml with manual sprayer to every strain, carries out the pathogeny inoculation.C, November 30, December 11 carry out 2 investigation results and see Table 2.
Table 1
| The method of control | Spray beginning day | Spray number of times | Spray at interval | |
| 1 district | The smart 500 times of aqueous solution of elscholtiza | November 17 | 2 times | 10 days |
| 2 districts | 500 times of aqueous solution of propineb aqueous solvent | November 17 | 2 times | 10 days |
| 3 districts | Non-processor |
Table 2
Annotate: (1) investigation method: 3 leaf investigation scab numbers are got in every strain, and the number of scab shown in the table 2 is every strain 3 leaves of 10 strains, the average scab number of every leaf of totally 30 leaves.(2) computational methods of the value of preventing and kill off: [(non-processor district value-treatment region value)/non-processor district value] * 100%, it prevents and kill off Value Data according to investigation result calculating on December 11.
| The scab number | The value of preventing and kill off | Poisoning | ||||
| The method of control | The investigation strain | November 30 | December 11 | |||
| 1 district | The elscholtiza essence | ????10 | ????10.9 | ????2.1 | ???88.5 | ???- |
| 2 districts | Propineb | ????10 | ????0.6 | ????0 | ???100 | ???- |
| 3 districts | Non-processor | ????10 | ????27.0 | ????18.3 | ||
Result of the test shows, Mosla chinensis has the control efficiency of the value of preventing and kill off 88.5% to strawberry anthracnose, there is no poisoning.
Embodiment 3 suppresses fungus grown and grows the test example
(1) experimental condition: 20 kinds of strains testeds, numbering (1)-(20), all are the fresh bacterial strains of the continuous 3 times above successive transfer culture of process for examination bacterium liquid;
(2) use 6 dosage of the smart aqueous solution of elscholtiza (dosage unit: the smart mg/ water of elscholtiza ml) 7.8,15.6,31.3,62.5,125 and 250;
(3) result's mensuration: following 18 hours of 37 ℃ of conditions of bacterium class, 28 ℃ of budding fungus 18 hours, 28 ℃ of fungies, because of the difference of different growth speed, minute is respectively after 24,48,72,96,120 hours.Result of the test sees Table 1.
Table 1
Annotate:the numeral in the table 1 is under fixed condition determination; mg, (-) ： ： ( 12 ) Rhizoctonia solani IFO 32364 ( 1 ) Staphylococcua aureus ATCC 6538 ( 7 ) Saccharomyces cerevislae ATCC 9763 ( 13 ) Phytophthola cactorum IFO32194 ( 2 ) Bacillus subtills ATCC 6633 ： ( 14 ) Sclerotinia sclerotiorum IFO 30965 ( 3 ) Escherichia coli NIIIJ ( 6 ) Aspergillus Miger ATCC 6275 ( 15 ) Botryotinia fuckeliana IFO 31831 ( 4 ) Pseudomonas aerugino 53 IFO 13736 ( 8 ) Trichophyton mentagrophytes IFO 32410 ( 18 ) Trichodetma viride IFO 30498 ( 5 ) Aeromonas hydrophila IFO l3286 ( 9 ) Fusarium solanif.sp.mori IFO 7707 ( 19 ) Helicobasidium mompa IFO 31651 ( 16 ) Erwinia carotovora IFO 14082 ( 10 ) Fusarium solanif.sp.mori IFO 9425 ( 20 ) Rosellinia necatrix IFO 9420 ( 17 ) Klebsiella pnevmoniae IFO 13277 ( 11 ) Cylindrocarpon destructans f.sp.panacis IFO 31881
| The test plant name | Strains tested | |||||||||||||||||||||
| ??No | Plant name | The position | ??(1) | ??(2) | ??(3) | ??(4) | ??(5) | ??(6) | ??(7) | ??(8) | ??(9) | ??(10) | ??(11) | ??(12) | ??(13) | ??(14) | ??(15) | ??(16) | ??(17) | ??(18) | ??(19) | ??(20) |
| ??3 | Wild marjoram (Fujian Province) | Herb | ??31.3 | ??125 | ??125 | ???- | ??31.3 | ???- | ??250 | ????- | ???- | ???- | ???- | ???- | ???- | ??250 | ???- | ???- | ??250 | ???- | ???- | ??125 |
| ??4 | Mongollian Thyme Herb (Shanxi Province) | Herb | ??62.5 | ??62.5 | ??125 | ???- | ??125 | ???- | ??62.5 | ????- | ???- | ??250 | ???- | ??125 | ??31.3 | ???- | ??125 | ???- | ???- | ??7.8 | ??250 | ??62.5 |
| ??6 | Mosla chinensis (Guangxi province) | Floral leaf | ??15.6 | ??62.5 | ??62.5 | ???- | ??62.5 | ??250 | ??7.8 | ??62.5 | ??125 | ??250 | ??125 | ??125 | ??125 | ???- | ??125 | ??125 | ???- | ??31.3 | ??250 | ??62.5 |
| ??8 | Mosla chinensis (artificial cultivation) | Floral leaf | ??31.3 | ??125 | ??125 | ???- | ??31.3 | ??62.5 | ??31.3 | ??125 | ??125 | ??125 | ??125 | ??31.3 | ??125 | ??125 | ??125 | ??31.3 | ??62.5 | ??125 | ??125 | ??31.3 |
| ??10 | Mosla chinensis (artificial cultivation) | The stem branch | ??62.5 | ??250 | ??250 | ???- | ??125 | ??250 | ??125 | ??250 | ???- | ???- | ???- | ????- | ???- | ????- | ??250 | ??250 | ????- | ???- | ???- | ??125 |
Embodiment 4 controls of aphids test example
(1) place: Dalian, the time: on June 2nd, 2000 and June 16 twice-daily use, to as if the apricot aphid, 500 times of aqueous solution and 1000 times of Rogor aqueous solution of using the elscholtiza essence that contains 9.6% Thymol compare.
(2) test method and result: selected 3 strain aphids to take place and the relatively more uniform apricot of distribution on June 2,500 times of aqueous solution with the elscholtiza essence that contains 9.6% Thymol spray 1 strain for 2 liters, 1000 times of Rogor aqueous solution spray 1 strain for 2 liters, each 1 liter of half and half strain that is sprayed on same strain apricot of 2 kinds of aqueous solution with above-mentioned concentration, after the sprinkling, observe the situation of living of aphid.After 2 days, the aphid that contains 500 times of aqueous solution of elscholtiza essence of 9.6% Thymol and the branch strain that 1000 times of Rogor aqueous solution sprays all is black and the coking shape is all dead.Sprayed 1 strain of 500 times of aqueous solution of the elscholtiza essence that contains 9.6% Thymol on June 16, come again same using, sprayed 1 strain of 1000 times of Rogor aqueous solution, do not do and repeat to spray, the 3 strain apricots of observing June 30 for examination all do not have aphid regeneration, but spray on the browse of 1000 times of Rogor aqueous solution slight poisoning are arranged.Result of the test shows that the present invention has practicality to control of aphids.
The artificial cultivation method of embodiment 5 Mosla chinensis
The cultivation time: year March in December, 1996-1997, place: Dalian.Carry out mid-December burying in seed low temperature and moisture about 30% sandy soil and put processing, the result is: be germination rate in (),-5 ℃ 10 days (2) ,-5 ℃ 20 days (48) ,-5 ℃ 30 days (52) ,-10 ℃ 10 days (0) ,-10 ℃ 20 days (51) ,-10 ℃ 30 days (47).After the low temperature treatment, without burying husky result be :-5 ℃ 10 days (0) ,-5 ℃ 20 days (12) ,-5 ℃ 30 days (11) ,-10 ℃ 10 days (0) ,-10 ℃ 20 days (7) ,-10 ℃ 30 days (6).Began to adopt-5 ℃ in 1997 in 2000 4 years and handled about one month and buried seeding and seedling raising after husky 20 days, germination is all about 50%.Conclusion is that low temperature treatment is the necessary condition of seed breaking dormancy, and buries the condition that certain hour is a seed sprouting of putting in the wet sandy soil.
The preparation method of embodiment 6 elscholtiza essences (methyl alcohol or ethanol extraction method)
(1) dry Mosla chinensis herb is pulverized, as 32 kilograms of raw materials; (2) to the methyl alcohol of 320 liter of 100% concentration of (1) lining injection, stir and soaked 20 minutes, carry out the first time and extract 273 kilograms of the extracts after the filtration; (3) be heated to 68 ℃, 204 liters of evaporation, recovery methyl alcohol obtain 69 kilograms of extracts; (4) methyl alcohol that will reclaim (3) increases by 80 liters for 204 liters again, injects the residue of (2) together, carries out soaking second time and extracts, and obtains 278 kilograms of extracts after the filtration; (5) method that repeats (3) reclaims 236 liters of methyl alcohol, obtains 42 kilograms of extracts; (6) (3) and (5) twice gained extract are mixed reduce pressure, heat, concentrate, obtain 4.15 kilograms of elscholtiza essences.This elscholtiza essence contains the water of 9.6% Thymol and 51.6%.Above operation all is to carry out in the utensil of sealing.
The preparation method of embodiment 7 elscholtiza essential oils (steam steams the slide method)
(1) dried Mosla chinensis herb is cut to 3 centimeters-5 centimeters long the section, weight is 78 kilograms; (2) (1) is put into distillation still, steam steamed in the below enters still 60 minutes; (3) pipeline that will discharge steam is introduced in the bosh, makes steam comb effluent oil water mixed liquid; (4) with oil and moisture from, obtain 1.7 kilograms of elscholtiza essential oils; (5) obtain that Thymol content is 59% in the elscholtiza essential oil, carvacrol content is 0.13%.
Claims (4)
1, application of the natural agent for preventing and eliminating Mosla chinensis of a kind of plant pest and production thereof and processing relate to the invention of protecting crops, belong to the agricultural plant protection field, Mosla chinensis need be carried out artificial cultivation when it is characterized in that being used to prevent and treat plant pest to obtain the Mosla chinensis herb and the effective component extracting of stable a large amount of cheapness, be Thymol and carvacrol, and make elscholtiza essence that contains Thymol or the elscholtiza essential oil that contains Thymol and carvacrol, this elscholtiza essence or elscholtiza essential oil and 500 times or 3000 times of water are mixed with the aqueous solution, can be used for preventing and treating cucumber downy mildew, strawberry anthracnose, tomato early blight, the tomato late blight, the eggplant cotton disease, the green pepper eqpidemic disease, tomato, capsicum, eggplant, cucumber, the gray mold of custard squash and melon downy mildew, powdery mildew, all obtain tangible control efficiency, for example, when the cucumber downy mildew of difficult control in control above-mentioned fruit and vegetable all diseases, for the every strain of cucumber blade all under the morbid state of adularescent spot from bottom to top, spray by strain, every 15 days, spray again, sprouting (axillalry bud) conservation rate is reached more than 80%; Also can be used for preventing and treating the insect pest of fruit aphid, to the fruit tree uniformly of taking place and distributing, spray by strain, every 15 days, spray again, the result is that aphid can not regenerate, the fruit tree spray, tender leaf does not come to harm yet, suppressing the bacterium class, yeast, during the growing of Mycophyta, even fresh bacterial strain through continuous 3 times above successive transfer culture, the flower of wild or tame Mosla chinensis, leaf or stem branch are under the various dose effect of the smart aqueous solution of the raw material elscholtiza of making, to suppressing yellow staphylococcus, the withered grass bacterium, colon bacillus, upright squama germ, Bacteria erwinia, yeast, black chrysanthemum is mould, the tinea alba bacterium, the mould germ of grey, the white root rot bacterium, Candida aldicans IFO 1594 (yeast class), Aeromonas salmonicida Sudsp-salmonicida 12659 (bacterium class), Fusarium oxysporum f.sp lycopersici IFO 31213 (Mycophytas) grows effectively, wherein, flower with Mosla chinensis, leaf is under the various dose effect of the smart aqueous solution of the raw material elscholtiza of making, to suppressing pine root fungus IFO7707, pine root fungus IFO9425, pine root fungus IFO31881, the seedling blight bacterium, epidemic disease bacterium, paddy rice rot bacterium, growing effectively of bavin brown root rot bacterium, the flower that also has tame Mosla chinensis, leaf is under the various dose effect of the smart aqueous solution of the raw material elscholtiza of making, to suppressing the sclerotium germ, growing effectively of pneumobacillus.
2, application of the natural agent for preventing and eliminating Mosla chinensis of the plant pest described in claim 1 and production thereof and processing, the artificial cultivation method that it is characterized in that Mosla chinensis is that the Mosla chinensis seed is placed-5 ℃--under 10 ℃, after 10 days-30 days, bury with wet sandy soil, after 20 days, seeding and seedling raising, germination rate are about 50%.
3, application of the natural agent for preventing and eliminating Mosla chinensis of plant pest described in claim 1 and production thereof and processing, it is characterized in that preparing the elscholtiza essence by Mosla chinensis is that the method for Mosla chinensis extract is that Mosla chinensis acrial part herb is carried out drying, after the pulverizing, adding the methyl alcohol or the ethanol that are equivalent to 10 times of raw materials stirs, behind extracting liquid filtering, heating, recovered under reduced pressure methyl alcohol or ethanol, obtain extract and residue for the first time, repeat for the first time same immersion and extract toward reinject methyl alcohol or ethanol of residue, the extract mixture of twice gained is lumped together heating, concentrating under reduced pressure obtains to contain the 9.0%-10.0% Thymol, the elscholtiza essence of about 50% water.
4, application of the natural agent for preventing and eliminating Mosla chinensis of the plant pest described in claim 1 and production thereof and processing, it is characterized in that preparing the elscholtiza essential oil by Mosla chinensis is that the method for volatile oil is to steam the slide method with steam, Mosla chinensis is contained volatile oil to steam with steam, with water-oil separating, obtain to contain the elscholtiza essential oil of 55.0%-60.0% Thymol and 0.10%-0.15% carvacrol.
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2000
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