CN105340985A - Protective and therapeutic fungus and nematode killing agent - Google Patents

Protective and therapeutic fungus and nematode killing agent Download PDF

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Publication number
CN105340985A
CN105340985A CN201510623035.5A CN201510623035A CN105340985A CN 105340985 A CN105340985 A CN 105340985A CN 201510623035 A CN201510623035 A CN 201510623035A CN 105340985 A CN105340985 A CN 105340985A
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China
Prior art keywords
killing
acid
nematode
fungi
killing agent
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CN201510623035.5A
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CN105340985B (en
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袁磊
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SHANGHAI HUMA GRO TECHNOLOGY Co Ltd
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SHANGHAI HUMA GRO TECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/22Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03Algae

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention discloses a protective and therapeutic fungus and nematode killing agent. The killing agent comprises thyme oil, organic acid, honey, glycerine and a weathering lignite dipping solution. The killing agent is used for controlling soil diseases and harm of parasitic nematodes on most crops. The killing agent can also improve activity of beneficial microorganisms so that the beneficial microorganisms can effectively inhibit diseases and nematodes. Meanwhile, the product provided by the present invention can also promote growth of plant roots and reduce the probability of secondary root diseases. The killing agent provided by the present invention is extracted from the thyme oil, belongs to biological pesticides and is environment-friendly.

Description

A kind of tool protectiveness and curative fungi, nematode agent for killing
Technical field
The invention belongs to field of biological pesticide, particularly a kind of tool protectiveness and curative fungi, nematode agent for killing.
Background technology
Along with people are to environmental protection and healthy concern, the use of the organophosphorous pesticide of efficient, high poison is all subject to restriction in various degree in each state.Efficiently, low toxicity, low-residual be the developing direction of agricultural chemicals industry, mainly comprises two large classes---the chemical pesticide of efficient, low toxicity and biopesticide.Biopesticide mainly refers to the agricultural chemicals with the secondary metabolite exploitation of agricultural biological activity produced with plant, animal, microorganism etc.Be used to anti-to cure the disease, worm, the biological living of the pest such as grass and metabolite thereof and transgene product, and the biogenic preparation of commodity listing can be made, comprise the genetically modified plants etc. of bacterium, virus, fungi, nematode, plant growth regulator and Resistant crop smothering.Biopesticide have selectivity strong, to people and animals' Environmental security, raw material sources extensively and not easily produce the advantages such as drug resistance, become the new trend of global pesticide development.After particularly Protocols in Molecular Biology, gene engineering etc. progressively penetrate in biopesticide production, the development of various countries to biopesticide is paid attention to more, and within significant period of time from now on, biopesticide will become an important directions of Agrochemicals from now on.
Summary of the invention
The object of this invention is to provide a kind of tool protectiveness and curative fungi, nematode agent for killing.
Technical scheme of the present invention is:
A kind of tool protectiveness and curative fungi, nematode agent for killing, comprise the raw material of following mass ratio:
(1) thyme linaloe oil 3%-20%;
(2) organic acid 5%-10%;
(3) honey 10%-45%;
(4) glycerine 10%-35%;
(5) fresh water algae powder, particle diameter 100-800 order, with dry weight basis 0.5%-5%;
(6) leonardite leaching liquor 10%-20%.
Preferred component comprises:
(1) thyme linaloe oil 5%-10%;
(2) organic acid 6%-9%;
(3) honey 15%-35%;
(4) glycerine 10%-20%;
(5) fresh water algae powder, particle diameter 100-800 order, with dry weight basis 1%-4%;
(6) leonardite leaching liquor 15%-25%.
Described fresh water algae powder diameter is preferably 300-500 order, and fresh water algae is preferably: chrysophyceae and xanthophyta.
Described organic acid is one at least in fulvic acid, amino acid, nucleic acid; Nucleic acid is at least one in DNA (deoxyribonucleic acid) and ribonucleic acid; Amino acid be selected from glutamic acid, aspartic acid one or more.
Described thyme linaloe oil is that labiate thyme enters (ThymusvulgarisL.) and enters the acrial part at florescence and obtain through steam distillation.
Above-mentioned tool protectiveness and curative fungi, nematode agent for killing also contain water: the total amount of thyme linaloe oil, organic acid, honey, glycerine, fresh water algae powder and leonardite leaching liquor and the weight ratio of water are 1:0.5-50.
The preparation method of this tool protectiveness and curative fungi, nematode agent for killing is: mix each component by proportioning and filter, getting filtrate, surplus water is supplied.
Fresh water algae is rich in protein, carbohydrate, chlorophyll, a large amount of mineral matters, vitamin, amino acid, nucleotide and other biological active substance, can provide nutrient for growth of microorganism.
Thyme linaloe oil is bronzing liquid, and refrigerant, the dry burnt herbal of tool, have the faint scent such as linalool, terpinene concurrently, principal component is Thymol (30% ~ 70%), carvacrol (2% ~ 15%).Mediterranean, the preferred place of production.
Fungi of the present invention, nematode killing agent put forward power from plant resource thyme linaloe oil, belong to safe biopesticide, for controlling the harm of the parasitic nematode of soil disease and most crops.Also can strengthen beneficial microorganism activity, make them can effectively suppress disease and nematode.This product also can promote plant root growth simultaneously, reduces the probability that secondary root catches.
The mechanism of action of the present invention is: control native Hereditary diseases and plant parasitic nematodes by action of contace poison.Can not enter in plant corpus, only be deposited on crop surface, shield.Preventive effect is stablized, and not easily brings out germ and nematode develops immunity to drugs.Its mechanism of action: be make mold fungi deformation of filament, suppress spore germination directly can kill spore to fungi; Be by increasing profitable strain in soil to the mechanism of action of nematode, suppress germ, nematode survival space, secretion is unfavorable for the metabolite that nematode, germ etc. survive.This product also can promote plant root growth simultaneously, reduces the probability that root system catches again.
The feature of tool protectiveness of the present invention and curative fungi, nematode agent for killing is as follows:
Safety is high.Safety interval is 0 day, and gathering in the crops interval is 0 day.
Broad-spectrum.Be a kind of contact bactericide of multiple-effect complexing, effectively can prevent and treat multiple diseases.The fungal diseases such as Fusarium, rotten mold genus, phytophthora, rhizoctonia, white rot, southern blight.
Not easily produce resistance.Control soil-borne disease and parasitic nematode by action of contace poison, can not enter in plant corpus, not easily bring out germ and nematode develops immunity to drugs.
Formulation aqua.Solution is more stable, and efficiency is higher, and using method is flexible, can be applicable to irrigation system.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
Following raw materials according mixed, add water to gross weight 1000kg, and in mixing kettle, stir filtration after 10 hours, the liquid obtained is fungi, nematode killing agent.
Thyme linaloe oil 35kg.
Organic acid 100kg, comprising fulvic acid 50kg, each 10kg of glutamic acid, aspartic acid (amounting to 20kg amino acid), DNA (deoxyribonucleic acid) and each 15kg of ribonucleic acid (amounting to 30kg nucleic acid).
Honey 150kg.
Glycerine 200kg.
Fresh water algae powder, particle diameter 100-800 order (dry weight) 1kg.
Leonardite leaching liquor 100kg.
Embodiment 2
Following raw materials according mixed, add water to gross weight 1000kg, and in mixing kettle, stir filtration after 10 hours, the liquid obtained is fungi, nematode killing agent.
Thyme linaloe oil 35kg.
Organic acid 130kg, comprising fulvic acid 50kg, each 10kg of glutamic acid, aspartic acid (amounting to 20kg amino acid), DNA (deoxyribonucleic acid) and each 15kg of ribonucleic acid (amounting to 30kg nucleic acid).
Honey 120kg.
Glycerine 150kg.
Fresh water algae powder, particle diameter 100-800 order (dry weight) 2kg.
Leonardite leaching liquor 130kg.
Embodiment 3
Using protectiveness obtained in embodiment 1 and curative fungi, nematode agent for killing as experimental subjects, on the soil of plantation potato, respectively to infecting sickle-like bacteria, the plot of rhizoctonia and natural infection soil carries out process and tests.2 process are established in the test of each plot, and process 1 is blank.Process 2 is fungi nematode agent for killing (PROMAX) of the present invention.Often process and repeat for three times.
The using dosage of PROMAX: 620 milliliters/mu, the water yield 15 liters/mu.
Table 1: different disposal crop contrast table
Disease Blank PROMAX (thousand grams/acre)
Sickle-like bacteria 208.3a 630.8b
Rhizoctonia 732.1a 977.1ab
Natural infection soil 1902.5a 2700.7b
Note: indicate same letter after data and represent otherness not significantly (DMRT Deng Kenshi multiregion method, p=0.05).
As shown in Table 1: the output infecting the potato blank of sickle-like bacteria is 208.3kg/ mu, is 630.8kg/ mu through PROMAX process potato yield, comparatively contrast volume increase 202.83%, both reach significant difference.The output infecting the potato blank of rhizoctonia is 732.1kg/ mu, and after PROMAX process, potato yield is 977.1kg/ mu, and process comparatively contrasts volume increase 33.47%.The output 1902.5kg/ mu of the potato blank of natural infection soil, the output through the potato of PROMAX process is 2700.7kg/ mu, and process comparatively contrasts volume increase 41.96%, and both reach significant difference.
Embodiment 4
Using protectiveness obtained in embodiment 2 and curative fungi, nematode agent for killing as experimental subjects, respectively process is carried out to the infection tomato of root-knot nematode and the soybean of Cyst nematode and test.Often kind of crop test establishes 2 process, and process 1 is blank.Process 2 is fungi nematode agent for killing (PROMAX) of the present invention.Often process and repeat for three times.
The using dosage of PROMAX: 620 milliliters/mu, the water yield 15 liters/mu.
Table 2: different disposal nematode population contrast table
Note: indicate same letter after data and represent otherness not significantly (DMRT Deng Kenshi multiregion method, p=0.05).
As shown in Table 2: the nematode in young age infecting the root of the tomato blank of nematode is 240.6, the nematode in young age through PROMAX process root is 65.8, and decline rate is 72.6%, and both reach significant difference.The nematode in young age infected in the soil of the tomato blank of nematode is 41, and the nematode in young age in PROMAX process soil is 4.9, and decline rate is 88%, and both reach significant difference.The quantity infecting the cyst in the root of the soybean blank of nematode is 8.4, and the cyst in PROMAX process root is 3.3, and decline rate is 71.4%, and both reach significant difference.The cyst infected in the soil in the soil of the soybean blank of nematode is 5.0, and the cyst in PROMAX process soil is 1.7, and decline rate is 66.0%, and both reach significant difference.
More than show and describe general principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.

Claims (7)

1. tool protectiveness and curative fungi, a nematode agent for killing, is characterized in that: the raw material comprising following percentage:
(1) thyme linaloe oil 3%-20%;
(2) organic acid 5%-10%;
(3) honey 10%-45%;
(4) glycerine 10%-35%;
(5) fresh water algae powder, particle diameter 100-800 order, with dry weight basis 0.5%-5%;
(6) leonardite leaching liquor 10%-20%.
2. tool protectiveness according to claim 1 and curative fungi, nematode agent for killing, is characterized in that, comprise the raw material of following quality:
(1) thyme linaloe oil 5%-10%;
(2) organic acid 6%-9%;
(3) honey 15%-35%;
(4) glycerine 10%-20%;
(5) fresh water algae powder, particle diameter 100-800 order, with dry weight basis 1%-4%;
(6) leonardite leaching liquor 15%-25%.
3. the tool protectiveness described in claim 1 or 2 and curative fungi, nematode agent for killing, it is characterized in that, described fresh water algae is chrysophyceae and xanthophyta, and powder diameter is 300-500 order.
4. the tool protectiveness described in claim 1 or 2 and curative fungi, nematode agent for killing, is characterized in that, described organic acid is one at least in fulvic acid, amino acid, nucleic acid.
5. tool protectiveness according to claim 4 and curative fungi, nematode agent for killing, is characterized in that, described nucleic acid is at least one in DNA (deoxyribonucleic acid) and ribonucleic acid; Amino acid be selected from glutamic acid, aspartic acid one or more.
6. the tool protectiveness described in claim 1 or 2 and curative fungi, nematode agent for killing, is characterized in that, described thyme linaloe oil is that labiate thyme enters the acrial part at florescence and obtains through steam distillation.
7. the tool protectiveness described in claim 1 or 2 and curative fungi, nematode agent for killing; it is characterized in that, also containing water: the total amount of thyme linaloe oil, organic acid, honey, glycerine, fresh water algae powder and leonardite leaching liquor and the weight ratio of water are 1:0.5-50.
CN201510623035.5A 2015-09-25 2015-09-25 One kind tool protectiveness and curative fungi, nematode agent for killing Active CN105340985B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108424335A (en) * 2017-02-15 2018-08-21 南京禾瑞中侬农业科技有限公司 A kind of carbon rush technology
CN111568926A (en) * 2020-06-03 2020-08-25 云南中翼鼎东生物医疗科技有限公司 Bacteriostatic agent containing lignite extracting solution and preparation method and application thereof
CN113767901A (en) * 2021-08-25 2021-12-10 河南农业大学 Application of thymol in prevention and treatment of peanut southern blight
CN114650732A (en) * 2019-08-22 2022-06-21 博塔诺健康有限公司 Mucilage based plant protection products and methods thereof

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CN102578155A (en) * 2012-01-11 2012-07-18 王广富 Peanut grub prevention and control agent and production method thereof
CN103553761A (en) * 2013-09-26 2014-02-05 上海优马生物技术有限公司 Functional nutrition agent for phytoremediation and its preparation method

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CN1357229A (en) * 2000-12-13 2002-07-10 刘岱 Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent
CN102578155A (en) * 2012-01-11 2012-07-18 王广富 Peanut grub prevention and control agent and production method thereof
CN103553761A (en) * 2013-09-26 2014-02-05 上海优马生物技术有限公司 Functional nutrition agent for phytoremediation and its preparation method

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108424335A (en) * 2017-02-15 2018-08-21 南京禾瑞中侬农业科技有限公司 A kind of carbon rush technology
CN114650732A (en) * 2019-08-22 2022-06-21 博塔诺健康有限公司 Mucilage based plant protection products and methods thereof
EP4017263A4 (en) * 2019-08-22 2023-08-16 Botanohealth Ltd A mucilage based plant protection product and method thereof
CN111568926A (en) * 2020-06-03 2020-08-25 云南中翼鼎东生物医疗科技有限公司 Bacteriostatic agent containing lignite extracting solution and preparation method and application thereof
CN113767901A (en) * 2021-08-25 2021-12-10 河南农业大学 Application of thymol in prevention and treatment of peanut southern blight

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