CN102617429A - Preparation method for athomin for prevention and treatment of rice planthopper - Google Patents
Preparation method for athomin for prevention and treatment of rice planthopper Download PDFInfo
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- CN102617429A CN102617429A CN2012100143970A CN201210014397A CN102617429A CN 102617429 A CN102617429 A CN 102617429A CN 2012100143970 A CN2012100143970 A CN 2012100143970A CN 201210014397 A CN201210014397 A CN 201210014397A CN 102617429 A CN102617429 A CN 102617429A
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Abstract
The present invention discloses a preparation method for an athomin insecticide for prevention and treatment of rice planthopper, wherein steps of the method comprise: crushing a plant containing athomin; adding the crushed plant to an extraction autoclave, starting a CO2 pressurzer, carrying out continuous extraction with a CO2 circulation method under the following conditions to obtain the athomin, wherein the pressure of a separation kettle I is 8-10 MPa, the temperature of the separation kettle I is 40-60 DEG C, the pressure of a separation kettle II is 5.6-6.0 MPa, the temperature of the separation kettle II is 30-50 DEG C, the CO2 flow rate is 1.0-2.0 kg/h, the extraction temperature is 30-55 DEG C, the extraction pressure is 20-30 MPa, and the extract time is 50-60 minutes. With the prepared athomin, the athomin emulsifiable concentrate insecticide and the athomin toxic soil insecticide are prepared. According to the present invention, the use amount of chemical pesticides in rice fields can be reduced, the pollution on the field environment due to agents can be reduced, other advantaged are provided, and good economic, social and ecological benefits can be provided.
Description
Technical field
The present invention relates to a kind of nuisance-free plant botanical pesticide---athomin is prevented and treated rice hopper, is specifically related to the preparation method of athomin missible oil and athomin pesticide-clay mixture.
Background technology
Brown paddy plant hopper and white backed planthopper are the insects of a kind of migrating property at a distance of Asia Dao Qu, also are the important pests on China paddy rice main producing region paddy rice, and rise the sixties in 20th century, brown paddy plant hopper in the Asia many country outbursts cause disaster, become the primary insect of these national paddy rice.China is since the seventies, and brown paddy plant hopper is taken place and harm is on the rise, and its characteristics one are that generation area and damaging range further enlarge; Take place the eighties and hazard area rises to about 200,000,000 mu, especially big generation in 1991, and generation and hazard area are above 3.4 hundred million mu; Account for 60% of national paddy rice cultivated area; The not only whole nation main rice district generation, serious harm, but also expand to over infrequent North China, northeast and northwest rice district; The 2nd, the outburst frequency obviously increases.Before the sixties, brown paddy plant hopper only endangers in local rice district, breaks out once in general 5～10 years, and general 5 years of the seventies was broken out once, has outburst in 9 years to cause disaster in 1980～1993 years 13 years, and its outburst frequency is respectively 10%～20%, 50% and 70%; The 3rd, hazard rating significantly increases the weight of.Brown paddy plant hopper great outburst in China in 1991, direct economic loss is up to 5,000,000,000 Renminbi.Though population quantity of brown paddy plant hopper and hazard rating descend to some extent over nearly 20 years, remain one of three major pests of China paddy rice main producing region, be the main attack object of chemical control.
China chemical insecticide control brown paddy plant hopper starts from late 1950s, and the sterilant of successively promoting has organochlorine, organic phosphorous insecticide and compound preparation, carbamate insecticides etc.The nineties, non-insects agent PP618 (buprofezin) the control brown paddy plant hopper large scale application of killing livestock made the chemical prevention of China brown paddy plant hopper get into a new stage.Thereafter the novel ultra-high efficiency chlorination nicotinic insecticide-Provado of exploitation,, special efficacy quick-acting to the Hefei City, consumption are few, become the main pesticide species of control brown paddy plant hopper rapidly.But because extensive, long-term, use continuously, brown paddy plant hopper has produced tangible resistance to Provado, and the control effect of Provado is sharply descended, and continues to seek new alternative pesticide species.
Along with sustainable agriculture theoretical proposition and people's showing great attention to food safety, environmental safety; Research and the application of plant insecticide in pest control; Receive scientist's extensive attention day by day, utilize pesticide plant and the good botanical pesticide of secondary metabolite development environment consistency thereof to become one of focus, emphasis of the research of current sterilant.The Secondary Metabolism of Plant product is to resist the insect's food-taking behavior in the long-term and insect coevolution process of plant and the chemical substance that produces, and these materials can pass through and be processed into plant insecticide.Plant insecticide has multiple biological activity to insect, and is little to natural enemy lethality, in environment, is prone to degraded, and insect numerous advantages such as be difficult for developing immunity to drugs meet the requirement of sustainable plant protection strategy to pesticidal properties.
Athomin is meant the one type of secondary metabolite that from cresss such as horseradish Armoracia lapathifolia, extracts; On chemical structure, all contain-compound of N=C=S reactive group; Wherein, effectively insecticidal constituent allyl group isosulfocyanate content is the highest.Both at home and abroad the research of athomin was mainly concentrated on the basic medical research aspect, discovering that athomin also has agriculture harmful organisms such as the agriculture pathogenic bacteria in farmland weed, the soil, Warehouse pest, nematodes and well tag and fumigation activity in recent years in the past.The a tree name reported in literature, the agriculture harmful organism of athomin control comprises weeds such as Maruta cotula, amaranthus, barnyard grass grass, ild avena sativa, Herba amaranthi ascendentis; Storage pests such as sitophilus zea-mais, confused flour beetle, lesser grain borer, red plan paddy rice and booklice; Soil such as cucumber root knot nematode disease, groundnut root knot nematode disease passes oxyuriasis; Silborne fungal diseases such as wheat root disease, pea root disease.But athomin missible oil preparation and be used to prevent and treat rice hoppers and be not reported so far.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, the plant insecticide of a kind of novel control rice hopper is provided, comprise the preparation and the method for use of athomin missible oil and pesticide-clay mixture.
Be used to prevent and treat the athomin method for producing insecticide of rice hopper, may further comprise the steps:
(1) will contain athomin and plant pulverizing, join in the extraction kettle, start CO
2Pressurizer carries out CO
2The circulation method continuous extraction, separation reactor I pressure 8--10MPa, 40--60 ℃ of separation reactor I temperature; Separation reactor I I pressure 5.6～6.0MPa; Separate 30--50 ℃ of II still temperature, the CO2 flow is 1.0-2.0kg/h, extraction temperature 30-55 ℃; Pressure 20-30MPa extracts under the condition of extraction time 50-60min and obtains athomin;
(2) preparation method of athomin cream pesticide:
Composition of raw materials is got according to volumetric enumeration under 20--25 ℃ of condition: athomin 20-50 part, emulsifying agent 5-15 part, solvent 40-70 part, and mix and stirred 10-30 minute, can obtain 20%--50% athomin cream pesticide;
(3) athomin pesticide-clay mixture method for producing insecticide:
Under 20--25 ℃ of air tight condition,, adopt progressively enlargement method that the two is fully mixed thoroughly and process the pesticide-clay mixture sterilant according to the sieve consumption of fine earth of every mu of 500～800mL athomin and 10--20Kg;
In the present invention, described emulsifying agent is 4103, and solvent is an ETHYLE ACETATE.
Athomin missible oil of the present invention or the pesticide-clay mixture control rice hopper optimum period for applying fertilizer be 2～3 age nymph.
Beneficial effect:
The present invention uses athomin and prevents and treats rice hoppers and compare with chemical pesticide, has following advantage:
1, crop diseases and pest, worm, grass are planted by double Zhiduo County.Athomin is a kind of broad-spectrum biological activity material; Existing research shows; Athomin not only has good insecticidal effect to storage pest, and multiple farmland weed, soil-borne disease, nematode are also had tangible control effect, and a dispenser can part-time multiple kinds of crops disease and pest and weeds.
2, high-efficiency low-toxicity is to the person poultry safety, little to the natural enemy lethal effect.Control rice hopper effect is higher than conventional pesticide.
3, insect is difficult for developing immunity to drugs.
4, raw material sources are extensive.Cresss such as horseradish are distributed widely in all parts of the country, but artificial culture, and living weight is big, can guarantee to propose the needs of athomin.
Embodiment
Be easy to understand and understand in order to make technique means of the present invention, creation characteristic, workflow, method of use reach purpose and effect,, further set forth the present invention below in conjunction with specific embodiment.
Embodiment 1, Toxicity Determination adopt medicine embrane method, topical application and fumigation and steaming method to measure the virulence of 92% athomin to brown paddy plant hopper nymph in 3 age.
Medicine embrane method:
Table 1 athomin to 3 age the brown paddy plant hopper nymph the effect of tagging (medicine embrane method)
Topical application:
Table 2 athomin to 3 age the brown paddy plant hopper nymph the effect of tagging (topical application)
Fumigation and steaming method:
Table 3 athomin to 3 age the brown paddy plant hopper nymph fumigation activity
Medicine embrane method and topical application method result show that 92% athomin is at medicine embrane method dosage 750-1250 μ g/cm
2Or every when receiving dose 7.5～12.5ug/, and 24 hours corrected mortalities are respectively 86.78%-97.55% and 88.47%-97.63% behind the medicine.Along with the prolongation of administration time, corrected mortality obviously improves, and 72 hours corrected mortalities are respectively 92.16～100.00% and 93.53%～100.00% (table 1 and table 2) behind the medicine.
Fumigation and steaming method is measured the result and is shown, when 92% athomin concentration was 2.5～3 μ L/L, the time of all knocking down brown paddy plant hopper nymph in 3 age only needed 10.38～9.5min (table 3)
Above-mentioned experimental result shows that 92% athomin not only has the very strong effect of tagging to brown paddy plant hopper nymph in 3 age, and also has good fumigating effect.
Embodiment 2, the potted plant control effect in solarium
The spray method efficiency test.
The preparation of athomin missible oil: 92% athomin crude oil, emulsifying agent (4103), solvent (ETHYLE ACETATE) were mixed with 20% athomin missible oil in 20: 10: 70 by volume, converted 5 concentration of water dilution then, spray value 60kg/667m
2Calculate.Access brown paddy plant hopper nymph in 3 age is 50 on pot rice.Different time inspection mortality ratio behind the medicine.The result shows, and is best with 30 times of liquid preventive effects, and behind the medicine 2 days and 3 days, mortality ratio was respectively 82.22% and 85.56%.(table 4)
The different extension rates of table 420% athomin missible oil are to the potted plant spraying mortality ratio (%) of brown paddy plant hopper nymph in 3 age
Annotate: the same treatment time of letter representation different concns extract in the table shows person's property to the difference between the mortality ratio of brown paddy plant hopper
Embodiment 3, pesticide-clay mixture efficiency test
Athomin pesticide-clay mixture preparation: the former medicine of 92% athomin mixed thoroughly by different amounts and fine earth processes pesticide-clay mixture, spread fertilizer over the fields in connect 3 age the brown paddy plant hopper nymph pot rice on, checked mortality ratio in 1,2,3 day behind the medicine, the result shows 800mL/667m
2The pesticide-clay mixture preventive effect that athomin is processed is best, and the mortality ratio of 3 days brown paddy plant hopper nymphs in 3 age reaches 82.22% behind the medicine.(table 5)
The different athomin consumptions of table 5 are to the potted plant pesticide-clay mixture mortality ratio (%) of brown paddy plant hopper nymph in 3 age
Annotate: the same treatment time of letter representation different concns extract in the table is to the significance of difference between the mortality ratio of brown paddy plant hopper
Embodiment 4, field efficiency test
20% athomin missible oil by the potted plant experimental technique preparation in solarium is released control brown paddy plant hopper nymph in 3 age under field condition for 30 times, and 1 day preventive effect is significantly higher than the preventive effect (73.45%) of 2000 times of liquid of 25% buprofezin up to 80.42% after the dispenser.3 days both preventive effects are suitable behind the medicine.(table 6)
The different chemicals treatment of table 6 to the brown paddy plant hopper nymph control effect
Athomin pesticide-clay mixture (700mL/667m by the potted plant experimental technique preparation in solarium
2) 1 day preventive effect 62.85% behind the medicine, be higher than conventional pesticide 80%DDV pesticide-clay mixture (150/667m
2) preventive effect of 3 days athomin pesticide-clay mixture but does not have significant difference a little less than the 80%DDV pesticide-clay mixture behind the medicine.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; The present invention is not restricted to the described embodiments; That describes in the foregoing description and the specification sheets just explains principle of the present invention; Under the prerequisite that does not break away from spirit and scope of the invention, the present invention also has various changes and modifications, and these variations and improvement all fall in the scope of the invention that requires protection.The present invention requires protection domain to be defined by appending claims and equivalent thereof.
Claims (4)
1. be used to prevent and treat the athomin method for producing insecticide of rice hopper, it is characterized in that, may further comprise the steps: will contain athomin and plant pulverizing, and join in the extraction kettle, and start CO
2Pressurizer carries out CO
2The circulation method continuous extraction, separation reactor I pressure 8--10MPa, 40--60 ℃ of separation reactor I temperature, separation reactor I I pressure 5.6～6.0MPa separates 30--50 ℃ of II still temperature, CO
2Flow is 1.0-2.0kg/h, and extraction temperature 30-55 ℃, pressure 20-30MPa extracts under the condition of extraction time 50-60min and obtains athomin.
2. the athomin method for producing insecticide that is used to prevent and treat rice hopper according to claim 1 prepares the athomin cream pesticide; It is characterized in that; Composition of raw materials is got according to volumetric enumeration under 20-25 ℃ of condition: athomin 20-50 part, emulsifying agent 5-15 part, solvent 40-70 part; Mix and stirred 10-30 minute, can obtain 20%--50% athomin missible oil.
3. the athomin method for producing insecticide preparation preparation horseradish pesticide-clay mixture sterilant that is used to prevent and treat rice hopper according to claim 1; It is characterized in that; Under 20--25 ℃ of air tight condition; According to every mu of 500～800mL athomin, the sieve consumption of fine earth of 10--20Kg adopts progressively enlargement method that the two is fully mixed thoroughly and processes pesticide-clay mixture.
4. the athomin method for producing insecticide that is used to prevent and treat rice hopper according to claim 1 is characterized in that described emulsifying agent is 4103, and solvent is an ETHYLE ACETATE.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012100143970A CN102617429A (en) | 2012-01-10 | 2012-01-10 | Preparation method for athomin for prevention and treatment of rice planthopper |
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| CN2012100143970A CN102617429A (en) | 2012-01-10 | 2012-01-10 | Preparation method for athomin for prevention and treatment of rice planthopper |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963734A (en) * | 2016-05-06 | 2016-09-28 | 云南联创利民生物工程有限公司 | Method for carrying out disinfection on interior of shed room by utilizing athomin |
| CN114040676A (en) * | 2019-07-05 | 2022-02-11 | 伊萨格罗股份公司 | Emulsifiable concentrate containing allyl isothiocyanate and application of emulsifiable concentrate as nematicide in crops |
| CN114586800A (en) * | 2022-03-02 | 2022-06-07 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and application thereof in prevention and control of spodoptera frugiperda |
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| WO2006102236A1 (en) * | 2005-03-18 | 2006-09-28 | Caudill Seed Inc. | Cancer chemoprotective compositions and natural oils and methods for making same |
| KR100834855B1 (en) * | 2007-03-09 | 2008-06-03 | 네오바이오 주식회사 | Feed for chicken containing extract from galic and coriandrum sativum, method for manufacturing the same, chicken feeding the same, and egg produced by the chicken |
| CN102190608A (en) * | 2010-03-12 | 2011-09-21 | 天津市食品研究所有限公司 | Method for extracting mustard oil from green radish peel |
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2012
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2006102236A1 (en) * | 2005-03-18 | 2006-09-28 | Caudill Seed Inc. | Cancer chemoprotective compositions and natural oils and methods for making same |
| KR100834855B1 (en) * | 2007-03-09 | 2008-06-03 | 네오바이오 주식회사 | Feed for chicken containing extract from galic and coriandrum sativum, method for manufacturing the same, chicken feeding the same, and egg produced by the chicken |
| CN102190608A (en) * | 2010-03-12 | 2011-09-21 | 天津市食品研究所有限公司 | Method for extracting mustard oil from green radish peel |
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| 吴华: "辣根植物杀虫杀菌活性精油的提取及应用研究", 《华中农业大学硕士学位论文》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963734A (en) * | 2016-05-06 | 2016-09-28 | 云南联创利民生物工程有限公司 | Method for carrying out disinfection on interior of shed room by utilizing athomin |
| CN114040676A (en) * | 2019-07-05 | 2022-02-11 | 伊萨格罗股份公司 | Emulsifiable concentrate containing allyl isothiocyanate and application of emulsifiable concentrate as nematicide in crops |
| CN114586800A (en) * | 2022-03-02 | 2022-06-07 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and application thereof in prevention and control of spodoptera frugiperda |
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Application publication date: 20120801 |