KR100834855B1 - Feed for chicken containing extract from galic and coriandrum sativum, method for manufacturing the same, chicken feeding the same, and egg produced by the chicken - Google Patents

Abstract

Poultry feed with the extracts of garlic and Coriandrum sativum, its preparation method, poultry fed on the feed, eggs laid by the poultry are provided to enhance the taste and nutrition of poultry, to increase the egg-laying rate, and to produce functional eggs low in saturated fatty acid. Poultry feed with the extracts of garlic and Coriandrum sativum is prepared by the steps of: (i) putting the garlic and Coriandrum sativum crushed in 3~5mm size into an extraction reactor of a supercritical fluid extractor; (ii) supplying CO2 as an extraction solvent from a fluid storage tank to the extraction reactor; (iii) keeping the pressure of CO2 supplied to the extraction reactor under 73~75bar and the temperature at 31~32°C for 50~60min to make an extract; (iv) reducing the pressure of the CO2 to 18~20bar to separate the CO2 from the extract; (v) transferring the separated CO2 to the fluid storage tank to supplement CO2, recirculating it to the extraction reactor, and collecting CO2 and the separated extract; and (vi) adding the extract to poultry feed made of corn, wheat, soybean cake, gluten meal, and beef tallow. The poultry feed comprises: 40~50wt.pt. of corn, 15~16wt.pt. of wheat, 25~28wt.pt. of soybean cake, 4.5~6.0wt.pt. of gluten meal, 4~4.5wt.pt. of beef tallow, 10~10.5wt.pt. of nutritive elements, and 0.02~0.05wt.pt. of the extract.

Description

Fowl forage with added extracts of garlic and hossil, its production method and poultry bred by feeding it and eggs produced by the poultry AND EGG PRODUCED BY THE CHICKEN}

1 is egg yolk test items of the control group of eggs produced by the control group of chickens fed the feed and the general feed fed the poultry feed was added to the poultry feed was added to the extract of garlic and lake oil according to the present invention Graph of test results showing test results for,

Figure 2 is a graph of the test results showing the test results for egg whites of the Harlan test item of Figure 1,

Figure 3 is a graph of the test results showing the test results for the Hohan unit of the Harlan test item of Figure 1,

4 is a graph showing test results for egg yolk among the test items of halan of FIG.

5 is a graph of experimental results of the Harlan test comprehensively showing the results of the experiments of FIGS. 1, 2, 3 and 4;

6 is a vitamin of the ingredient test items of the control group of the egg produced by the control group of chickens fed the feed and the general feed of eggs produced by the feeders of poultry farms fed with the extract of garlic and lake oil room according to the present invention Graph of test results showing test results for A,

7 is a graph showing the results of the test for vitamin E among the component test items of FIG. 6,

8 is an experimental result graph showing a test result for cholesterol among the component test items of FIG. 6,

FIG. 9 is a graph showing experimental results of a component test overall result which collectively shows the experimental results of FIGS.

10 is a graph showing the results of measuring the immunological activity against the bacteria in the cells of the control group fed a poultry feeder and a general poultry feed fed poultry feed added to the extract of garlic and hoyusil according to the present invention,

Figure 11 is a graph showing the results of the immunological activity measurement for the virus tested in the laboratory of the control group fed the poultry feeder and general feed fed chicken poultry feed supplemented with extracts of garlic and horse oil room according to the present invention ,

Figure 12 shows the results of the measurement of the immune activity against the intracellular bacteria tested in the field of the control group fed the poultry feeder and feeder fed broiler poultry feed was added to the extract of garlic and hoyusil according to the present invention It is a graph.

The present invention relates to a poultry feed, a method of manufacturing and a poultry raised by feeding them and the eggs produced by the poultry, in particular, by adding the extract extracted through the supercritical fluid extraction process from garlic and lake oil in the feed to improve the immunity of poultry Poultry feed supplemented with garlic and hoyusil extract, which lowers cholesterol levels, and enhances eggshell eggshell egg.

In the current situation of increasing interest in the environment in all processes throughout the industry, the process of extracting and refining active ingredients of natural products is also a problem in the existing extraction and purification processes using organic solvents, namely environmental and human toxicity, and target components. Attempts are being made at home and abroad to introduce new processes that can address non-selectivity and high cost.

As a result, technology using supercritical fluid as a process fluid is rapidly spreading to various industries such as fine chemicals, energy, environment, and new materials, and is replacing various traditional separation technologies with new technologies using supercritical fluid processes.

In the supercritical fluid extraction technology, all pure materials show the phase of gas and liquid solids according to the change of temperature and pressure. Looking at the vapor pressure curve, which is the phase transition curve of gas and liquid, the pressure increases with temperature, which results in an increase in the vapor pressure as a result of reaching a new equilibrium point, which in turn reduces the difference in density between the liquid phase and the gas phase. . These liquid and gas density differences are equal at the critical point and are indistinguishable. In the region above the critical point, no liquefaction occurs even when the pressure is increased, and no vaporization occurs when the temperature is increased.

Above this critical point, a fluid having an intermediate nature between gas and liquid is defined as a supercritical fluid. Supercritical fluids vary greatly in density, even at small temperature and pressure changes, making it easy to control dissolving power and have other unique characteristics from gases and liquids.

In other words, it is characteristic of liquid in terms of dissolution related to the interaction between solvent and solute molecules, density, which is closely related to the ability to separate solute from matrix, and related to substrate permeability. High diffusivity, low surface tension, etc., indicate the properties of the gas.

Supercritical Fluid Extraction Technology is a technology that uses fluids above the critical temperature and pressure to replace existing processes in the extraction and refining fields of pharmaceuticals, food processing and petrochemical refining. It is attracting attention as an environmentally friendly clean technology. In particular, in advanced countries, gas and liquids have been used in traditional processes for the past 30 years due to the rise of energy resource prices, the environmental hazards of traditional separation processes, and the expansion of demand for special purpose new materials that cannot be manufactured by gas or liquid processes. We have been devoting ourselves to the development of new process fluid technology that uses the supercritical fluid technology as a process fluid, avoiding the concept of using.

 Supercritical fluid extraction is a technology that takes advantage of the advantages of supercritical fluids and has a number of unique advantages because it is a complex technology in which the principles of distillation and extraction are applied together. Since supercritical fluids can be set to any condition from high density to low density by operating pressure temperature, it has excellent selectivity for fractionation and separation, so that high purity products can be obtained and the extraction solvent can be recovered almost completely without loss. And a purified product with no residual solvent can be obtained.

In addition, the viscosity of the supercritical fluid is small, so it penetrates into the sample, and the extraction efficiency is high. Also, the diffusion coefficient is large, so the extraction speed is high. Extraction at a relatively low temperature can avoid damage due to heat. Since the density difference with the critical fluid is large and the viscosity of the supercritical fluid is low, the separation residue and the solvent are easily separated and have many advantages. However, since the use of a high pressure device has a disadvantage in that facility cost and maintenance cost are high, extraction using a supercritical fluid is known to be economical only if it is made with high efficiency.

In addition, garlic contains not only a role as a spice but also a useful ingredient for regulating biological functions, which is known as a beneficial food for health maintenance. The functionality of garlic is allicin (diallylthiosulfinate), methyl allyl, 1-propenyl allyl, allyl 1-propenyl, 1-propenyl methyl (1- Thiosulfinate compounds such as propenyl methyl and dimethyl thiosulfinate play the greatest role, and these include anti-thrombotic, anticancer, lowering blood pressure, lowering cholesterol and anti-aging. have. Allicin ([C3H5-S-S (O) -C3H5]), which is a major component of thiosulfinate compound, has many biochemical activities such as antibacterial activity and inhibition of cell metabolism of garlic.

Therefore, a lot of efforts are being made to utilize this functionality of garlic in our country and all over the world.

Related patents related to the present invention are related to chicken feed and chickens. Patent No. 10-0466609-0000, 'Method of preparing chicken feed with garlic, chicken feed produced therefrom, and garlic chicken fed by feeding The gist of this patent is as follows. The present invention provides a method for preparing chicken feed containing 0.3 to 1.0% by weight of dried garlic powder, and antibiotics based on corn, wheat, wheat blood, soybean meal, and ox gluten, and garlic-containing chicken feed produced therefrom. And garlic chickens fed with this feed.

However, looking at the processing step for adding garlic powder to the feed in the present invention, it is well known that the modification or efficacy of the component is poor if the heat treatment process is already undergoing a steam drying process, so this also useful components of garlic It can be seen that there is a disadvantage that is destroyed.

In particular, in an environment where chickens are raised in large quantities, as in Korea, the damages caused by stress and diseases (Newcastle disease, infectious bronchitis, Marek's disease, Salmonella disease, avian influenza) are very fatal. Therefore, in order to cure and prevent these diseases, antibiotics and various drugs have to be provided to poultry, so there is a significant cost burden. In addition, fishmeal is used as a raw material of conventional poultry feed. Eggs produced by laying poultry hens fed these feeds have an inevitable fishy fishy smell and are easily deteriorated due to short storage periods. It is known to provide a cause of asthma.

Therefore, in consideration of the above points, the heat-free treatment method, such as the supercritical fluid extraction technique mentioned above, removes the spicy taste of garlic, reduces the rejection of poultry, and extracts it with the most active ingredients and adds it to poultry feed. By improving the immunity enough to replace the use of antibiotics, and also improve the meat quality of the poultry, there is a need for a method that the active ingredient is contained in the eggs produced by the poultry fed the feed to produce a functional egg.

Therefore, the present invention is to solve the problems of the prior art, the purpose is to improve the immunity of chickens, lower cholesterol levels, eggs of the egg by adding the extract extracted through the supercritical fluid extraction process from garlic and lake oil chamber to feed The present invention provides a poultry feed supplemented with an extract of garlic and horse oil, which has an eggshell strengthening effect, a method of preparing the same, and a poultry bred and fed eggs.

A feature of the present invention is a pulverized powder input step of injecting garlic and arc oil crushed to the size of the particle size 3 ~ 5mm inside the extraction reactor of the supercritical fluid extraction device, carbon dioxide which is an extraction solvent from the fluid storage tank in the extraction reactor ( CO ₂₎ for supplying a solvent in step, the extraction step of maintaining 50-60 minutes the pressure of the carbon dioxide (CO ₂₎ supplied to the inside of the extraction reactor by 73 ~ 75bar, 31 ~ 32 ℃ temperature extract the extract, Extraction separation step of separating the CO ₂ and the extract by reducing the pressure of the carbon dioxide (CO ₂ ) from the extract to 18 ~ 20bar, the separated carbon dioxide (CO ₂ ) is moved to the fluid storage tank lost carbon dioxide (CO ₂₎ a supplementary step after the extract collected to collect the cycle again supplied to the extraction reactor and to separate the carbon dioxide (CO ₂₎ extract and corn, wheat, soybean meal, wheat gluten, a poultry feed for beef tallow as a raw material There to were added to extract group provides a process for the preparation of poultry feed mixture prepared garlic extract and call the lost containing feed is added to the manufacturing stage.

In the extracting step, extracting the extract under the condition of maintaining the pressure of CO ₂ supplied into the extraction reactor at 73.7 bar and the temperature at 31.2 ° C. for 60 minutes may be preferable because the amount of extraction is greatest.

The poultry feed is 40 to 50 parts by weight of corn, 15 to 16 parts by weight of wheat, 25 to 28 parts by weight of soybean meal, 4.5 to 6.0 parts by weight of gluten wheat, 4 to 4.5 parts by weight of beef fat, 10 to 10.5 parts by weight of other nutritional ingredients, It contains 0.02-0.05 weight part.

In addition, the other nutrients include calcium phosphate, limestone, salt, lysine, methionine, choline chloride, vitamin E, breeder vitamin P (Breeder Vit PX) and poultry min (Poultry Min PX) in combination of two or more selected Good to do.

In addition, another feature of the present invention, the poultry feed made by the method of manufacturing a poultry feed to which the extract of the garlic and lake oil room is added, and the poultry and poultry eggs raised to ingest the poultry feed To provide.

(Example)

Hereinafter, the present invention will be described in detail with reference to the following Examples, but the present invention is not limited by the Examples.

Attached Figure 1 is a Harlan test item of the control was a egg produced by the control group of chickens fed the feed and the general feed of the eggs produced by the feeder of the poultry feeder was added to the extract of garlic and lake oil in accordance with the present invention Experimental results graph showing the test results for the weight of the egg, Figure 2 is a graph of the test results showing the results of the egg white test of the Harlan test item of Figure 1, Figure 3 is a test result of the Hofu unit of the Harlan test item of Figure 1 4 is a graph showing the results of the test for egg yellow among the test results of harlan in FIG. 1, and FIG. 5 is a total of the harlan test showing the experimental results in FIGS. 1, 2, 3 and 4 comprehensively. Experimental results graphs for the results, Figure 6 is a feeder and days of the eggs produced by the feeders of broiler chickens fed feed of the extract of garlic and lake oil in accordance with the present invention Experimental result graph showing the test results for vitamin A among the component test items of the control group of the egg, which is a control group of chickens fed feed fed, Figure 7 is a graph showing the test results for vitamin E among the ingredient test items of Figure 6 8 is an experimental result graph showing test results for cholesterol among the component test items of FIG. 6, and FIG. 9 is an experimental result graph for comprehensive test results of the compound test results of the test results of FIGS. 6, 7 and 8, and FIG. 10 is a graph showing the results of measuring the immune activity against the intracellular bacteria of the control group fed the poultry feeder and the feed broiler fed the poultry feed added to the extract of garlic and hoyusil according to the present invention, Figure 11 Garlic and broiler chickens fed with poultry feed supplemented with garlic and hoyusil extracts A graph showing the results of measuring the immunological activity against the virus tested in the laboratory of the control group, Figure 12 is fed to the poultry feed broiler fed the garlic and lake oil extracts according to the present invention were fed to the feed group and general feed breeding It is a graph showing the results of measuring immune activity against intracellular bacteria tested in the field of the control system of the poultry.

Supercritical Fluid Extraction Apparatus used in the present invention is generally divided into four parts. First, the fluid reservoir, the source of high-purity supercritical carbon dioxide, is primarily a high-purity liquefied carbon dioxide cylinder on a laboratory scale. Second, there is a high pressure pump that can adjust the pressure and flow rate constant or easily, and in the case of the high pressure pump, a check valve is attached to prevent the backflow of the pressure pushed into the reactor. Third, the sample is contained in the extraction tank, which is installed in the thermostat because the constant temperature must be maintained. Fourth, a restrictor keeps the required pressure in the extraction tank constant. Fluid reservoirs are mainly cylinders of steel, aluminum or stainless steel. The pressure in the cylinder is high enough for the contents to be liquid. The high pressure pumps used in supercritical fluid extraction are similar to those used in HCP or supercritical fluid chromatography. However, no device for increasing the pressure or density used in supercritical fluid chromatography is required. For optimal performance, a pressure of 70 to 700 bar should be obtained, and the operating pressure should be kept constant and measured immediately. It should be reproducible at 1.0 ~ 100ml / min and no pulse at the flow rate. Extraction tanks are places where supercritical fluids can come into contact, where unknown samples are placed. In order to be used effectively in supercritical fluid extraction, several conditions must be met.

In such a supercritical fluid extraction device, the ability to dissolve the nonvolatile mixture of the supercritical fluid and the rapid change in solubility according to the pressure can reduce the energy requirement during liquid extraction or distillation. The solvent power of the supercritical fluid is important for the selective extraction of the desired material. To this end, the supercritical fluid can be controlled by varying many options such as pressure, temperature and solvent.

Particularly, in the present invention, carbon dioxide (CO ₂ ) was used as an extraction solvent, which is a solvent mainly used in supercritical fluid extraction, and after extraction, it is evaporated under atmospheric pressure to be released into the atmosphere. It can be used to make products free from contamination in the food and pharmaceutical manufacturing industries. Liquid extraction leaves residual toxic organic solvents after distillation or vacuum separation steps. Compared to the density and solvent capacity of organic solvents, the density and solvent capacity of supercritical fluids have higher transfer efficiency, such as lower viscosity and higher diffusivity. Therefore, even though the various components as oligomers have a similar volatility, the extract has various advantages that can be separated into each component. In the present invention, a supercritical fluid extraction apparatus using carbon dioxide (CO ₂ ) as an extraction solvent is used.

In addition, garlic has a large amount of aryl sulfides and derivatives thereof, which has effects on taste, smell, and preservation, and contains various components such as sugars and essential amino acids. As the effective ingredients have been proven in various ways, they are widely applied as special ingredients such as industrial products, cosmetics, and medicines in addition to food.

In the active ingredient of garlic, allin (S-allyl L-cysteine Sulfoxide), a group of sulfur-containing amino acids, is decomposed into allicin and pyruvic acid by its own enzyme allinase, and allicin is decomposed into diallyl sulfide to obtain lower sulfide.

In addition, the lower sulfide, an active ingredient of garlic, acts to delay or inhibit the fat and oil of fats and oils by sulphating the fats and oils in foods. It has the effect of sterilization, and its value is increasing today.

In addition, Hoyu-sil is also called coriander, which is used as a medicine called 'Ho-yu-sil' in Chinese medicine, which makes the stomach strong, digestive, stops coughing, eliminates bad breath, and heals wounds. In addition, it helps to cure anemia, benefit the large and small intestine, make the abdomen feel, fever extremities, and treat headaches. In addition, Hoyusil's seeds are effective in worm poisoning, hemorrhoids, meat poisoning, hemorrhage, bleeding, boiling juice, squeezing oil, and applying them to children's soles. In addition, it is recorded that the effect of diarrhea, diarrhea, headache, toothache, pain, dizziness, digestive action, bile secretion, antifungal action, indigestion, inedible treatment.

Accordingly, the present invention minimizes the deformation or destruction of the active ingredient by using a supercritical fluid extraction device, garlic and lake oil having a component having many effects as described above to efficiently extract and mix the raw material of poultry feed The results of this study were to provide a poultry feed supplemented with garlic and hoyusil extract, a method for preparing the same, and a poultry bred and fed eggs.

The following describes the high-efficiency extraction method of garlic and lake oil chamber using the supercritical fluid extraction technology according to the present invention in order. In the present invention, a supercritical fluid extraction device was used SFX3560 of ISCO. First, the garlic and arc oil chamber crushed to a size of 3 to 5 mm are introduced into the extraction reactor of the supercritical fluid extracting device, and then the carbon dioxide (CO ₂ ) pressurized to the nozzle (valve) is passed through a heat exchanger to the lower layer through a pump. The pressure from the reactor is controlled by a back pressure regulator and maintained at a constant temperature through the heater surrounding the reactor.

In addition, the extraction solvent CO ₂ is extracted in an upflow manner in contact with the garlic and the arc oil chamber from the lower layer, wherein the temperature and pressure inside the extraction reactor are 73 to 75 bar in the critical range condition of CO ₂ . , Extract the temperature at 31-32 ℃ for 50-60 minutes. At this time, the extraction of the extract under the condition of maintaining the pressure of CO ₂ supplied into the extraction reactor at 73.7 bar and the temperature at 31.2 ° C. for 60 minutes may be more preferable since the extraction amount is the most. The reason why the holding temperature is 31 to 32 ° C. is because when the temperature exceeds 32 ° C., the density decreases and the solubility in the solute decreases, thereby decreasing the extraction amount.

Thereafter, the extracted extract is separated into CO ₂ and the extract in a separator maintained at about 18-20 bar under reduced pressure while passing through the backflare valve through the upper valve, and the carbon dioxide separated from the phase change from the liquid phase to the gas phase. It is separated and flows through the heat exchanger into the fluid reservoir. In addition to the circulated supply of CO ₂ , the fluid storage tank may be further supplied with CO ₂ for supplementation from the outside so as to compensate for some loss occurring in the entire process. The separated extract is added to corn, wheat, soybean meal, gluten wheat, tallow, etc. as raw materials of poultry feed to prepare poultry feed.

The poultry feed is 40 to 50 parts by weight of corn, 15 to 16 parts by weight of wheat, 25 to 28 parts by weight of soybean meal, 4.5 to 6.0 parts by weight of gluten wheat, 4 to 4.5 parts by weight of tallow, 10 to 10.5 parts by weight of other nutritional ingredients, It contains 0.02 ~ 0.05 parts by weight of the extract extracted from the garlic and lake oil.

In addition, the other nutrients are selected from calcium phosphate, limestone, salt, lysine, methionine, choline chloride, vitamin E, Breeder Vit PX and Poultry Min PX for nutrient supplementation. It is preferable to further include a combination of two or more.

Poultry feed prepared by the above method was configured as shown in Table 1 to be ingested over three weeks in a laying poultry.

Kinds 1 week 2 weeks 3 weeks Compounding ratio (parts by weight) Compounding ratio (parts by weight) Compounding ratio (parts by weight) corn 40.70 45.96 49.12 Wheat 15.00 15.00 15.00 Soybean meal 28.02 25.37 21.27 Gluten wheat 6.00 4.56 5.51 Uji 4.50 4.00 4.00 Calcium phosphate 1.91 1.81 1.67 Limestone 1.28 1.30 1.42 saline 0.25 0.25 0.25 Lysine 1.50 0.97 0.95 Methionine 0.31 0.25 0.28 Corinated Chloride 0.10 0.10 0.10 Vitamin E 0.10 0.10 0.10 Breeder Vitamin Premix 0.15 0.15 0.15 Poultry Premix 0.15 0.15 0.15 Extract of Garlic and Hossil 0.02-0.05 0.02-0.05 0.02-0.05

In order to confirm the results, the blood test, the Harlan test and the component test were divided into a salary group and a non-salary group who consumed the feed according to the present invention.

A total of 22 test items were tested with plasma and serum samples for 6 minutes using the VET TEST of IDEXX, the world's most widely used animal blood chemistry analyzer.

First, the mean blood chemistry of the pay group is shown in Table 2, and the mean blood chemistry of the non-pay group is shown in Table 3.

division unit Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Average Albumin (ALB) 100ml / g 1.5 1.4 1.1 1.5 2.2 1.54 Alkaline phosphatase (ALKP) U / L 76.0 90.0 92.0 101.0 45.0 80.8 Alkaliaminotransferase (ALT) <10 <10 <10 <10 12.0 Amylase (AMYL) 339.0 258.0 291.0 231.0 453.0 314.4 Urea Nitrogen (BUN) 100ml / mg 2.0 2.0 2.0 2.0 2.0 2.0 Calcium (Ca ²⁺ ) > 16 > 16 > 16 > 16 15.6 Cholesterol (CHOL) 131.0 113.0 123.0 127.0 239.0 146.6 Creatinine (CREA) 0.1 0.1 0.1 0.1 0.1 0.1 Glucose (GLU) 251.0 240.0 227.0 231.0 264.0 242.6 Weapon (PHOS) 6.2 7.4 5.9 7.2 5.8 6.5 Total bilirubin (TBIL) 0.3 0.4 0.4 0.2 <0.1 0.28 Total Protein (TP) 100ml / g 5.5 5.3 4.7 5.8 6.6 5.58 Globulin (GLOB) 4.0 3.9 3.6 4.3 4.4 4.04

division unit Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Average Albumin (ALB) 100ml / g 1.3 1.2 1.3 1.3 1.2 1.26 Alkaline phosphatase (ALKP) U / L 595.0 378.0 740.0 460.0 489.0 532.4 Alkaliaminotransferase (ALT) 19.0 <10 <10 15.0 <10 Amylase (AMYL) 276.0 318.0 266.0 257.0 313.0 286.0 Urea Nitrogen (BUN) 100ml / mg 2.0 2.0 2.0 2.0 2.0 2.0 Calcium (Ca ²⁺ ) > 16 > 16 > 16 > 16 > 16 Cholesterol (CHOL) 88.0 133.0 123.0 108.0 99.0 110.2 Creatinine (CREA) 0.1 0.1 0.1 0.1 0.1 0.1 Glucose (GLU) 246.0 218.0 238.0 217.0 229.0 229.6 Weapon (PHOS) 2.9 4.8 7.9 4.4 5.5 5.1 Total bilirubin (TBIL) 0.2 0.1 0.3 0.1 0.4 0.22 Total Protein (TP) 100ml / g 5.0 4.8 5.4 4.6 5.0 4.96 Globulin (GLOB) 3.7 3.6 4.1 3.3 3.8 3.70

Alkali phosphatase (ALKP) among the blood chemistry items shown in Tables 2 and 3 is a category that can be referred to as a guideline for evaluating liver function, and the salary group is significantly lower than that of the control group, and globulin (GLOB) can measure immune formation ability. As a possible item, the number of the paying group is higher than that of the control group, indicating that it is superior in the formation of immunity. In order to certify the effect of improving immunity, serum was separated from the poultry in each of the feeder and control group, and the immunological activity was measured by immunofluorescent antibody staining using lymphocytes (a type of lymphocyte among white blood cells). Are shown as control graphs in FIGS. As shown in FIG. 10, the specific immunity against the bacteria in the cells tested in the laboratory was significantly improved compared to the control group, which showed that the immunity against the virus of FIG. 11 had the same effect. In addition, in the immunity test outside the laboratory, that is, outdoors, as shown in FIG. 12, it was confirmed that the immunity of the pay group was improved than the control group.

In addition, the Harlan test was carried out at the Livestock Grading Institute in the Korean Poultry Farm. The results are shown in Table 4 below. Eggs produced by the pay group were labeled as feed and control eggs as controls.

division Salary Control unit I'm in 70.25 68.02 gram I'm back 7.47 8.19 mm Hofu Unit 82.21 87.61 HU Egg yellow 9.33 8.80

In the egg yolk test items shown in Table 4, the egg yolk in egg yolk and egg yolk, which is a criterion of consumer selection, showed a higher value than the control group, indicating that only high quality eggs are produced as a result. 2, egg white in FIG. 3, Hofu unit in FIG. 3, egg yellow in FIG. 4, and a graph of the experimental results for the results of the Harlan test in FIG.

In addition, the component test was conducted by the Korea Food Research Institute. The cholesterol analysis test method was tested according to the International Chemical Analytical Standards (AOAC) Official Method 994.10, and the component analysis test of vitamin A and vitamin E was described in the Food Code (2006). Test by micronutrient test method showed the results as shown in Table 5.

division Salary Control unit Remarks Vitamin A 1451.9 1317.3 IU / 100mg 10% increase Vitamin E 0.2 0.6 mg / 100mg cholesterol 369.5 554 mg / 100mg 34% reduction

As can be seen from the component analysis results shown in Table 5, vitamin A has a 10% increase in the feed, and cholesterol, which is a measure of the saturated fatty acid content in eggs, has a 34% reduction in egg value. It can be seen that the results of the test results for the results of the vitamin A in FIG. Clearly confirmed.

The present invention extracts the active ingredients from garlic and lake oil using supercritical fluid extraction method and then adds them to poultry feed, strengthening the poultry's immunity, resistant to disease, excellent in taste and nutrition, laying egg laying rate It has an excellent effect of raising it.

In addition, the eggs produced by poultry produced by poultry feed prepared by the method of manufacturing poultry feed supplemented with the extracts of garlic and lake oil improved egg weight, eggshell thickness and blue strength, and markedly increased cholesterol levels. Lowering has the advantage of producing a functional egg with a very low saturated fatty acid content in eggs. Although the present invention has been described with reference to the above embodiments, it is merely illustrative, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. . Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

Claims (7)

A pulverized product input step of inputting garlic and an arc oil chamber crushed to a size of 3 to 5 mm in the extraction reactor of the supercritical fluid extraction device; A solvent input step of supplying carbon dioxide (CO ₂ ), which is an extraction solvent, from the fluid storage tank in the extraction reactor; Component extraction step of extracting the extract by maintaining the pressure of the CO ₂ supplied into the extraction reactor at 73 ~ 75bar, temperature at 31 ~ 32 ℃ 50 ~ 60 minutes; Extract separation stage for separating CO ₂ by the pressure of the extract and the CO ₂ pressure to 18 ~ 20bar from the extract; The separated CO ₂ is moved to a fluid storage tank to replenish the lost CO ₂ and then circulated again to the extraction reactor, the extract collection step of collecting the extract separated from the CO ₂ ; And Feed preparation step of adding the extract separated from the CO ₂ to poultry feed based on corn, wheat, soybean meal, gluten wheat, tallow; Garlic and hoyusil extract containing a method of producing poultry feed. The method according to claim 1, wherein the component extraction step The method of producing a poultry feed is added to the extract of garlic and hoyusil to extract the extract by maintaining the pressure of CO ₂ supplied into the extraction reactor at 73.7bar, the temperature at 31.2 ℃ 60 minutes. The method of claim 1, wherein the poultry feed 40 to 50 parts by weight of corn, 15 to 16 parts by weight of wheat, 25 to 28 parts by weight of soybean meal, 4.5 to 6.0 parts by weight of gluten wheat, 4 to 4.5 parts by weight of beef tallow, 10 to 10.5 parts by weight of other nutritional ingredients, the extract 0.02 to 0.05 Including a weight part, the other nutrients are at least two selected from calcium phosphate, limestone, salt, lysine, methionine, choline chloride, vitamin E, breeder Vit PX and Poultry Min PX A method of producing a poultry feed to which the extract of garlic and hoyusil containing a combination is added. delete Poultry feed produced by the method of producing a poultry feed to which the extract of garlic and hoyusil of claim 1 added. Poultry bred by ingesting the poultry feed produced by the method of manufacturing a poultry feed to which the extract of garlic and hoyusil of claim 5 added. Eggs produced by poultry bred by ingesting poultry feed produced by the method of producing a poultry feed to which the extract of garlic and hoyusil of claim 6 added.

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