CN104938853B - A kind of preparation and its application of quality broiler chicken additive - Google Patents
A kind of preparation and its application of quality broiler chicken additive Download PDFInfo
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- CN104938853B CN104938853B CN201510366994.3A CN201510366994A CN104938853B CN 104938853 B CN104938853 B CN 104938853B CN 201510366994 A CN201510366994 A CN 201510366994A CN 104938853 B CN104938853 B CN 104938853B
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Abstract
Preparation and its application process the present invention provides a kind of quality broiler chicken additive.This product is made of following raw material components：5 15 parts of clover flavones, 2 10 parts of pine needle polysaccharide, 25 40 parts of cornstarch.Wherein pine needle polysaccharide is made of drying-crushing-ultrasonic wave assisted water extraction-concentrating and purifying-freeze-drying.Clover flavones is by drying-crushing-supercritical CO2Fluid extraction-concentration-level Four purifying-freeze-drying is made.Manufactured additive premix product is added in broiler fodder with 0.5% dosage.Clover flavones is extracted using this method, flavones yield and purity improve, and save the time, have saved reagent.The pine needle polysaccharide and clover flavones that this additive product uses are natural plant extracts, and raw material sources are stable and extensive, do not contain antibiotic composition, have no toxic side effect, drug residue free.Used using this additive product in feed, the immunity of broiler chicken can be strengthened, improve feed digestion metabolic rate, reduce feed-weight ratio, reduce abdominal fat, improve meat, increase culture benefit, and drug resistance and antibiotic residue problem is not present.
Description
Technical field：The invention belongs to broiler chicken feed additive field, and in particular to a kind of pine needle polysaccharide and clover flavones
The methods for making and using same of compound additive.
Background technology：
With the development of intensive culture industry, antibiotic residue and chicken meat quality are declined to become influence broiler production development
Key issue.Plant extracts because it is natural, low toxicity, residual are few, definite effect and paid close attention to be subject to the producer, current high-quality
Paid attention in broiler production research.
Pine needle is the needle of the pine trees such as masson pine, Pinus taiwanesis, and in China, annual yield surpasses hundred million tons.The crude protein of pine needle
Matter, crude fat content are enriched, and also have 9 kinds of essential amino acids and unrighted acid needed for animal, and multivitamin, micro-
Secondary element.Pine needle based on remove pathogenic factor, do not give birth to by Xie Quze Zhengan County, disease, so as to obtain the actual effect of not hungry macrobiosis.《Compendium of Materia Medica》
Speech, pine needle is long-term use of, controls all kinds of diseases and ailments, five viscera settling, piliation, resistance to cold and heat is resistance to expose to wind and rain, and QI invigorating of making light of one's life by commiting suicide, wards off paddy and prolong in keeping
Year.Just it is used in early in the last century 70's end pine needle meal in feed additive production, and achieves and be preferably produced into
Achievement.Clover system pulse family clover category herbaceos perennial, is known as the good reputation of " King of Pasture ".It is cultivated extensively, and nutritive value is rich
Richness, suffered chemical contamination is few, has been used as the high-quality feed of livestock and poultry.But pine needle and clover are up to more than 30%
Crude fibre, and special odor inhibits feeding of the nonruminant to pine needle and clover, reduces the nonruminants pair such as pig, chicken
Its digestibility and utilization, limits further utilization of this kind of feed in Production of Livestock and Poultry.
Pine needle polysaccharide and clover flavones are a kind of bioactive substances extracted respectively from pine needle and clover, be pine needle and
Important active functional component in clover.Research shows that plant polyose has growth promotion, anti-oxidant, anti-aging, antiviral and rush
Into functions such as immune functions, plant flavone is promoting growth of animal, raising human body immune function, adjusting animal lipid to be metabolized, carry
Also there is its unique effects in terms of high livestock products quality.This product extracts polysaccharide compound from pine needle, then is extracted from clover
Flavone compound, the advantages of then in conjunction with both pine needle polysaccharide and clover flavones, passes through broiler fodder prepared by compounded technology
Additive product.This product advantage is that raw material sources are stablized extensively, has and improves animal immunizing power, promotes chicken growth, subtracts
Few Abdominal Fat in Broilers rate, improves the effect of meat quality of table poultry, and small to the toxic side effect of body.
The content of the invention：
It is an object of the invention to provide a kind of quality broiler chicken with pine needle polysaccharide clover flavones type addictive preparation method and
Application process.The clover flavones preparation method can improve flavones yield and purity.The additive can improve broiler chicken and exempt from
Epidemic disease power, improve efficiency of feed utilization, adjusts broiler chicken lipid metaboli, improves chicken meat quality.
For achieving the above object, the technical solution adopted by the present invention is：A kind of preparation of quality broiler chicken additive,
It is made of and is made the raw material of following parts by weight：5~15 parts of clover flavones, 2~10 parts of pine needle polysaccharide, 25~40 parts of cornstarch.
2nd, the preparation method of pine needle polysaccharide：
The first step：Gather fresh pine needle branches and leaves to be placed at shady and cool ventilation, disleave is simultaneously shredded, 70~80 DEG C of drying, extremely
When moisture is down to 18%~22%, discharging crushed 40 mesh sieves.
Second step：Pine needle meal and hot water are pressed 1：10~20 solid-liquid ratio mixing, in 90~100 DEG C of Extracting temperature, pH value
Under conditions of 8~9,20KHz ultrasonic wave 40~60min of assisted extraction, after vacuum filtration, obtain the filter containing pine needle polysaccharide
Liquid.
3rd step：By above-mentioned gained filtrate after rotary evaporator concentrates, purified using Amberlyst process, obtain pine
Pin polysaccharide extraction liquid.
4th step：Obtained pine needle polysaccharide extracting solution is dispensed into refrigerator tray, in -80 DEG C of refrigerator freeze 24~
36h.The good sample of pre-freeze is placed on drying frame, sets -40 DEG C of pre-freezing temperature, 18~20pa of drying chamber pressure, drying time
24~30h.Obtain pine needle polysaccharide dry powder.
Its technique is pine needle-drying-crushing-ultrasonic wave assisted water extraction-concentrating and purifying-freeze-drying-pine
Pin polysaccharide.
3rd, the preparation method of clover flavones：
The first step：By fresh alfalfa stem and leaf 120 DEG C of enzyme deactivations 15~20min, 60~80 DEG C of 24~30h of drying, after crushing
Cross 40 mesh sieves.
Second step：The Alfalfa Meal of crushing carries out degreasing and decolorization using soxhlet extraction method.By what is handled well
Alfalfa loads supercritical CO2In the Raw material pail of fluid extraction apparatus, each valve is regulated, it is 30MPa to make extracting pressure,
Extraction temperature is 40~50 DEG C, and separating pressure is 8~10MPa, and separation temperature is 40~50 DEG C.CO2Flow control 30~
40kg/h, adds absolute alcohol as entrainer, extracts 1.5~2h on this condition, obtain clover flavones extract.
3rd step：The clover flavones extract of acquisition is transferred in rotary evaporator and is concentrated, thickening temperature is adjusted to
90~100 DEG C, 140~150 turns/min of rotating speed, obtain flavones concentrate.
4th step：The good clover flavones concentrate of above-mentioned concentration is added and is equipped with the splitter for handling DM130 resins well,
PH be 4~5, blade diameter length ratio 1:20~25, under conditions of loading flow velocity is 2mL/min, desorption flow velocity is 1.5mL/min,
Eluted with 1.5~2 times 70% of ethanol solution, by level Four after purification, collect eluent.
5th step：By the eluent of collection by freeze-drying, -40 DEG C of pre-freezing temperature, 15~20pa of drying chamber pressure, does
Dry 30~36h of time.Obtain clover flavones dry powder.
Its technique is alfalfa stem and leaf-drying-crushing-supercritical CO2Fluid extraction-concentration-level Four purifying-freezing
Drying-clover flavones.
4th, 5-15 parts of clover flavones, 2-10 parts of pine needle polysaccharide, 25-40 parts of cornstarch are crushed and uniformly mixed, cross 60
Mesh sieve.
5th, manufactured additive premix product is added in broiler fodder with 0.5% dosage.
Compared with prior art, the advantages and positive effects of the present invention are：
1st, the CO that the present invention uses2Supercritical extract combination macroreticular resin level Four purification technique extracts clover flavones, time saving,
Extractant is saved, flavones yield and purity are all improved.
2nd, the pine needle polysaccharide and clover flavones that the present invention selects, is all natural plant extracts, does not contain antibiotic, nontoxic
Noresidue, there is no drug resistance.
3rd, the present invention plays the joint effect of plant polyose and plant flavone by pine needle polysaccharide and clover flavone compounded,
Chicken immunity can be improved, feed-weight ratio is reduced, adjusts animal lipid metaboli, improves meat.
Embodiment：
Technical scheme is described in further detail with reference to embodiment
Embodiment 1：
The first step：Gather fresh pine needle branches and leaves to be placed at shady and cool ventilation, disleave is simultaneously shredded, 70 DEG C of drying, to moisture
When being down to 18%~22%, discharging crushed 40 mesh sieves.
Second step：Pine needle meal and hot water are pressed 1：10 solid-liquid ratio mixing, in 100 DEG C of Extracting temperature, pH value is 9 condition
Under, 20KHz ultrasonic wave assisted extraction 40min, after vacuum filtration, obtain the filtrate containing pine needle polysaccharide.
3rd step：By above-mentioned gained filtrate after rotary evaporator concentrates, purified using Amberlyst process, obtain pine
Pin polysaccharide extraction liquid.
4th step：Obtained pine needle polysaccharide extracting solution is dispensed into refrigerator tray, 24h is freezed in -80 DEG C of refrigerator.Will
The good sample of pre-freeze is placed on drying frame, sets -40 DEG C of pre-freezing temperature, drying chamber pressure 18pa, drying time 30h.Obtain pine
Pin polysaccharide dry powder.The pine needle polysaccharide purity that the method obtains is more than 70%.
5th step：By fresh 120 DEG C of enzyme deactivation 15min of alfalfa stem and leaf, 70 DEG C of drying 24h, cross 40 mesh sieves after crushing.
6th step：The Alfalfa Meal of crushing carries out degreasing and decolorization using soxhlet extraction method.By what is handled well
Alfalfa loads supercritical CO2In the Raw material pail of fluid extraction apparatus, each valve is regulated, it is 30MPa to make extracting pressure,
Extraction temperature is 50 DEG C, separating pressure 8MPa, and separation temperature is 40 DEG C.CO2Flow control is in 30kg/h, addition absolute alcohol
As entrainer, 2h is extracted on this condition, obtains clover flavones extract.The clover flavones yield of the method is more than 13mg/
g。
7th step：The clover flavones extract of acquisition is transferred in rotary evaporator and is concentrated, thickening temperature is adjusted to
95 DEG C, 150 turns/min of rotating speed, obtain flavones concentrate.
8th step：The good clover flavones concentrate of above-mentioned concentration is added and is equipped with the splitter for handling DM130 resins well,
PH be 5, blade diameter length ratio 1:25th, under conditions of loading flow velocity is 2mL/min, desorption flow velocity is 1.5mL/min, with 1.5 times
70% ethanol solution elution, by level Four after purification, collects eluent.
9th step：By the eluent of collection by freeze-drying, -40 DEG C of pre-freezing temperature, drying chamber pressure 15pa, when dry
Between 36h.Obtain clover flavones dry powder.The clover flavones purity that the method obtains is more than 27%.
Embodiment 2：
The first step：Gather fresh pine needle branches and leaves to be placed at shady and cool ventilation, disleave is simultaneously shredded, 80 DEG C of drying, to moisture
When being down to 18%~22%, discharging crushed 40 mesh sieves.
Second step：Pine needle meal and hot water are pressed 1：10 solid-liquid ratio mixing, in 90 DEG C of Extracting temperature, pH value is 9 condition
Under, 20KHz ultrasonic wave assisted extraction 60min, after vacuum filtration, obtain the filtrate containing pine needle polysaccharide.
3rd step：By above-mentioned gained filtrate after rotary evaporator concentrates, purified using Amberlyst process, obtain pine
Pin polysaccharide extraction liquid.
4th step：Obtained pine needle polysaccharide extracting solution is dispensed into refrigerator tray, 24h is freezed in -80 DEG C of refrigerator.Will
The good sample of pre-freeze is placed on drying frame, sets -40 DEG C of pre-freezing temperature, drying chamber pressure 20pa, drying time 24h.Obtain pine
Pin polysaccharide dry powder.The pine needle polysaccharide purity that the method obtains is more than 70%.
5th step：By fresh 120 DEG C of enzyme deactivation 15min of alfalfa stem and leaf, 75 DEG C of drying 24h, cross 40 mesh sieves after crushing.
6th step：The Alfalfa Meal of crushing carries out degreasing and decolorization using soxhlet extraction method.By what is handled well
Alfalfa loads supercritical CO2In the Raw material pail of fluid extraction apparatus, each valve is regulated, it is 30MPa to make extracting pressure,
Extraction temperature is 40 DEG C, separating pressure 10MPa, and separation temperature is 50 DEG C.CO2Flow control adds no watery wine in 40kg/h
Essence is used as entrainer, extracts 1.5h on this condition, obtains clover flavones extract.The clover flavones yield of the method is more than
13mg/g。
7th step：The clover flavones extract of acquisition is transferred in rotary evaporator and is concentrated, thickening temperature is adjusted to
95 DEG C, 140 turns/min of rotating speed, obtain flavones concentrate.
8th step：The good clover flavones concentrate of above-mentioned concentration is added and is equipped with the splitter for handling DM130 resins well,
PH be 5, blade diameter length ratio 1:20th, under conditions of loading flow velocity is 2mL/min, desorption flow velocity is 1.5mL/min, with 2 times
70% ethanol solution elution, by level Four after purification, collects eluent.
9th step：By the eluent of collection by freeze-drying, -40 DEG C of pre-freezing temperature, drying chamber pressure 20pa, when dry
Between 30h.Obtain clover flavones dry powder.The clover flavones purity that the method obtains is more than 27%.
Verification the verifying results：
It is yellow to the clover of the present invention by embodiment 1 and embodiment 2 compared with Hot water extraction and ultrasonic wave auxiliary alcohol extracting method
The extraction of ketone and purification effect are verified.
Using common water extraction and ultrasonic wave auxiliary alcohol extracting method as contrast, the extracting flavonoids by Examples 1 and 2 to the present invention
Yield is studied：
Group I：The good alfalfa meal of dries pulverizing and degreasing, decolorization is added to 40 times of hot water, 4h is extracted at 90 DEG C.
Obtain clover flavone extractive.By the rotated evaporation and concentration of flavone extractive, obtained concentrate, which loads, has been loaded with what is handled well
In the chromatographic column of Silon, eluted with 70% ethanol of 3 times of volumes with the flow velocity of 0.5mL/min, eluent is freeze-dried
Into powder.
Group II：By the good alfalfa meal of dries pulverizing and degreasing, decolorization, add 40 times 70% of ethanol, in 200W work(
Under rate, with 30kHz ul-trasonic irradiations, when extraction 3 is small under the conditions of 80 DEG C.Obtain clover flavone extractive.Flavone extractive is passed through
Rotary evaporation concentrates, and obtained concentrate is fitted into the chromatographic column for the Silon for having been loaded with handling well, with the 70% of 4 times of volumes
Ethanol is eluted with the flow velocity of 0.5mL/min, and eluent is freeze-dried into powder.
Group III：Clover flavones dry powder is obtained by embodiment 1.
Group IV：Clover flavones dry powder is obtained by embodiment 2.
Using rutin as standard items, using colorimetric method for determining flavones content, and flavones yield and purity are calculated.
Influence (mg/mL) of 1 distinct methods of table to clover flavones yield and purity
Table 1 the result shows that, with hot water extraction polyamide chromatography technology and ultrasonic wave auxiliary alcohol extracting polyamide chromatography technology
Compare, the flavones yield of embodiment 1 and embodiment 2 improves 0.5~1.3 times, and purity improves 0.61~1.1 times.And save
Time, saves reagent.
Embodiment 3：The pine needle polysaccharide dry powder and clover flavones dry powder obtained according to the method for embodiment 1 or example 2, by clover
10 parts of flavones, 5 parts of pine needle polysaccharide, 35 parts of cornstarch are uniformly mixed, and are crossed 60 mesh sieves and are obtained additive product.
Embodiment 4：The pine needle polysaccharide dry powder and clover flavones dry powder obtained according to the method for embodiment 1 or example 2, by clover
5 parts of flavones, 5 parts of pine needle polysaccharide, 40 parts of cornstarch are uniformly mixed, and are crossed 60 mesh sieves and are obtained additive product.
Embodiment 5：The pine needle polysaccharide dry powder and clover flavones dry powder obtained according to the method for embodiment 1 or example 2, by clover
15 parts of flavones, 10 parts of pine needle polysaccharide, 20 parts of cornstarch are uniformly mixed, and are crossed 60 mesh sieves and are obtained additive product.
Verification the verifying results：
Embodiment 3 is added in quality broiler chicken feed with 0.5% ratio, takes feeding experiment and metabolic test,
Using nonreactive feed and broiler feed as contrast, the growth promoting function of additive of the present invention is studied.
Experimental animal：30 age in days health Chongren Chicken corss combination systems similar in weight are female 360 young, are randomly divided into three
Group, every group of 4 repetitions are each to repeat 30.It is one week to test pre-feeding period, feeds basal diet, and basal diet is：Corn
64.56%, dregs of beans 24.80%, fish meal 3.00%, wheat bran 3.32%, rapeseed oil 1.00%, calcium monohydrogen phosphate 0.74%, mountain flour
1.38%, salt 0.20%, premix 1.00%.It is positive to raise the phase as 28 days.Free choice feeding and drinking-water during whole experiment.
Group I：Control group, does not add antibiotic and product of the present invention in basal diet.
Group II：Antibiotic control group, in basal diet plus sulfur acid colistin 0.5%, does not add product of the present invention.
Group III：Test group, 3 product 0.5% of embodiment is added in basal diet.
The production performance of 2 Chongren Chicken of table
From table 2, compared with control group, embodiment 3 improves the weight and daily gain of Chongren Chicken, reduces material weight
Than, its effect close to antibiotic control group.
The nutrient metabolizability (%) of 3 Chongren Chicken of table
By table 3 as it can be seen that the chicken of feeding embodiment 3 can improve the metabolic rate of dry matter in feed, thick protein, it is metabolized
The raising degree of rate is even above metabolic rate fatty in feed antibiotic group, but difference is not notable close to antibiotic group
(P>0.05)。
The Biochemical Indices In Serum of 4 Chongren Chicken of table
Blood parameters are related with the metabolism of chicken.Feed total protein and globulin content in the chicken serum of embodiment 3
Pole is significantly higher than control group and antibiotic group (P<0.01), and hdl concentration is significantly higher than control group (P<0.05),
Low-density lipoprotein pole is substantially less than control group and antibiotic group (P<0.01), lipoprotein lipase is also significantly lower than control group (P<
0.05).Illustrate that this product has effect in terms of immunity is improved, adjust lipid metaboli.
The Immune Organs Index of 5 Chongren Chicken of table
The immunological indices of 6 Chongren Chicken of table
From table 5 and table 6, example example 3 has no significant effect (P to the Immune Organs Index of Chongren Chicken>0.05), but
Extremely significantly (P<And notable (P 0.01)<0.05) concentration of blood middle leukocytes interleukin 2 and tumor necrosis factor is improved, is said
Raising of the bright this product to chicken immunity is mainly in haematogenic immunity index.
The content of fat in body of 7 Chongren Chicken of table
The muscle chemical composition of 8 Chongren Chicken of table
By table 7 and table 8 as it can be seen that 3 pole of embodiment significantly reduces the abdominal fat (P of chicken<0.01) trunk, is significantly reduced
Subcutaneous fat thickness and intramuscular fat width (P<0.05), extremely significantly (P<0.01) and significantly (P<0.05) reduce chest muscle and
Fat content in leg flesh.
More than result of the test show that add feed addictive of the present invention, daily gain is slightly below antibiotic group, but be higher than pair
According to group.Feed-weight ratio is less than control group, slightly above antibiotic group.Compared with control group, product of the present invention does not have Immune Organs Index
Have an impact, mainly by improving the content of globulin in blood, interleukin 2 and tumor necrosis factor etc., improved to play
The effect of immunity of organisms.Product of the present invention can also adjust chicken lipid metaboli, significantly increase high-density lipoprotein, reduce it is low
Density lipoprotein, reduces serum cholesterol levels, and then reduces carcass lipid deposition, reduces fat content in chest muscle, leg flesh,
Improve meat.The present invention tests prove that can substitute antibiotics medicine, significantly improve the haematogenic immunity function of broiler chicken, improve and raise
Expect utilization rate, improve daily gain, reduce feed-weight ratio, adjust the lipid metaboli of broiler chicken, reduce carcass lipid deposition, improve meat.
Claims (2)
- A kind of 1. preparation method of quality broiler chicken additive, it is characterised in that：It is made of and is made the raw material of following parts by weight：Lucerne 5-15 parts of Mu flavones, 2-10 parts of pine needle polysaccharide, 25-40 parts of cornstarch；Clover flavones, pine needle polysaccharide, cornstarch are crushed And uniformly mix, cross 60 mesh sieves；Preparing for two kinds of raw materials is as follows：The preparation method of pine needle polysaccharide：The first step：Gather fresh pine needle branches and leaves to be placed at shady and cool ventilation, disleave is simultaneously shredded, 70~80 DEG C of drying, to moisture When being down to 18%~22%, discharging crushed 40 mesh sieves；Second step：Pine needle meal and hot water are pressed 1：10 ~ 20 solid-liquid ratio mixing, in Extracting temperature 90 ~ 100oC, pH value are 8 ~ 9 Under the conditions of, 20KHz ultrasonic wave 40 ~ 60min of assisted extraction, after vacuum filtration, obtain the filtrate containing pine needle polysaccharide；3rd step：By above-mentioned gained filtrate after rotary evaporator concentrates, purified using Amberlyst process, it is more to obtain pine needle Sugared extracting solution；4th step：Obtained pine needle polysaccharide extracting solution is dispensed into refrigerator tray, 24 ~ 36h is freezed in -80 DEG C of refrigerator；In advance The sample frozen is placed on drying frame, sets -40 DEG C of pre-freezing temperature, 18 ~ 20pa of drying chamber pressure, 24 ~ 30h of drying time； To pine needle polysaccharide dry powder；Its technique is more for pine needle-drying-crushing-ultrasonic wave assisted water extraction-concentrating and purifying-freeze-drying-pine needle Sugar；The preparation method of clover flavones：The first step：By fresh 120 DEG C of 15 ~ 20min of enzyme deactivation of alfalfa stem and leaf, 60 ~ 80 DEG C of 24 ~ 30h of drying, cross 40 mesh after crushing Sieve；Second step：The Alfalfa Meal of crushing carries out degreasing and decolorization using soxhlet extraction method；It is pale reddish brown by what is handled well Clover loads supercritical CO2In the Raw material pail of fluid extraction apparatus, each valve is regulated, it is 30 MPa to make extracting pressure, extraction It is 40 ~ 50 DEG C to take temperature, and separating pressure is 8 ~ 10 MPa, and separation temperature is 40 ~ 50 DEG C；CO2Flow control in 30 ~ 40kg/h, Absolute alcohol is added as entrainer, 1.5 ~ 2h is extracted on this condition, obtains clover flavones extract；3rd step：The clover flavones extract of acquisition is transferred in rotary evaporator and is concentrated, thickening temperature is adjusted to 90 ~ 100 DEG C, 140 ~ 150 turns/min of rotating speed, obtain flavones concentrate；4th step：The good clover flavones concentrate of above-mentioned concentration is added and is equipped with the splitter for handling DM130 resins well, in pH For 4 ~ 5, blade diameter length ratio 1:20 ~ 25, under conditions of loading flow velocity is 2 mL/min, desorption flow velocity is 1.5 mL/min, use 1.5 ~ 2 times 70% of ethanol solution elution, by level Four after purification, collects eluent；5th step：By the eluent of collection by freeze-drying, -40 DEG C of pre-freezing temperature, 15 ~ 20pa of drying chamber pressure, when dry Between 30 ~ 36h；Obtain clover flavones dry powder；Its technique is alfalfa stem and leaf-drying-crushing-supercritical CO2Fluid extraction-concentration-level Four purifying-freezing is done Dry-clover flavones.
- 2. the preparation method of quality broiler chicken additive according to claim 1, it is characterised in that：Manufactured additive is pre- Mix product is added in broiler fodder with 0.5% dosage.
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| CN105851502A (en) * | 2016-04-22 | 2016-08-17 | 安徽金冠农牧科技有限公司 | Meat quality improving additive capable of maintaining wateriness and juiciness of muscle fibers and preparation method of meat quality improving additive |
| CN115093899A (en) * | 2022-06-23 | 2022-09-23 | 承德隆宏生物科技有限公司 | Production process of pine needle essential oil |
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