KR20110002621A - Manufacturing method of granular tea using phellinus linteus - Google Patents

Manufacturing method of granular tea using phellinus linteus Download PDF

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KR20110002621A
KR20110002621A KR1020090060173A KR20090060173A KR20110002621A KR 20110002621 A KR20110002621 A KR 20110002621A KR 1020090060173 A KR1020090060173 A KR 1020090060173A KR 20090060173 A KR20090060173 A KR 20090060173A KR 20110002621 A KR20110002621 A KR 20110002621A
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weight
phellinus linteus
concentrate
granular tea
tea
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KR1020090060173A
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KR101100337B1 (en
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최정화
김경진
송원영
김민선
김지연
정영철
강신권
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성병훈
한국국제대학교 산학협력단
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/163Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/18Extraction of water soluble tea constituents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/22Drying or concentrating tea extract
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/34Tea substitutes, e.g. matè; Extracts or infusions thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Dispersion Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

PURPOSE: A producing method of functional granular tea using phellinus linteus is provided to enable an eater to easily intake the phellinus linteus by granulating an extract of the phellinus linteus and medical herbs. CONSTITUTION: A producing method of functional granular tea using phellinus linteus comprises the following steps: hydrothermal extracting the phellinus linteus, and concentrating; hydrothermal extracting medical herbs including paeonia lactiflora, Korean angelica root, rehmanniae radix preparata, ganoderma, cassia seeds, zingiberis rhizoma crudus, cnidii rhizoma, jujube, cinnamon, and licorice, and concentrating; sterilizing the phellinus linteus extract and the medical herbs extract; detecting the proper pH, and inspecting for bacterium, before mixing with an additive containing glucose hydrocrystalline and lactose; granulating the mixture using a granulator; and drying the obtained granules.

Description

상황버섯을 활용한 기능성 과립차 제조방법{Manufacturing method of granular tea using Phellinus linteus}Manufacturing method of granular tea using situational mushrooms {Manufacturing method of granular tea using Phellinus linteus}

본 발명은 건강식품으로 널리 알려진 상황버섯을 그 기능성을 그대로 보존하면서도 쉽게 섭취할 수 있도록 과립차로 제조하는 방법에 관한 것이다.The present invention relates to a method for producing granular tea so that the situation mushroom, widely known as a health food, can be easily consumed while preserving its functionality.

21세기 고령화 시대의 건강화두가 단순한 수명연장에서 노화방지로 옮아가고 있다. 또한 2020년이 되면 전 세계 국민 절반 이상이 고령화 사회가 된다고 하며 고령화 사회로 접어들면 필연적으로 여러 가지 만성퇴행성 질환의 예방과 치료에 대한 대책이 필요할 것이다. 또한 인간은 연령의 증가에 따른 노화현상으로서 생리적 기능이 저하되고 면역기능이 약화됨으로써 동맥경화, 고혈압, 당뇨병 등 여러 가지 퇴행성 질환을 동반하고 이에 부응하여 전 세계적으로 항노화(anti-aging) 관련연구가 활발히 진행되고 있고 관련시장 또한 활발한 성장세를 타고 있다.Health issues in the aging age of the 21st century are moving from simple life extension to anti-aging. In addition, by 2020, more than half of the world's citizens will become aging societies, and inevitably, measures to prevent and treat various chronic degenerative diseases will be necessary. In addition, as the aging of human beings increases with age, the physiological function is decreased and the immune function is weakened. Therefore, humans are accompanied by various degenerative diseases such as arteriosclerosis, hypertension, diabetes, and anti-aging researches worldwide. Is actively progressing, and the related markets are also actively growing.

이러한 결과 식품소재 중에 존재하는 활성성분을 이용, 인간에 내재하는 생체방어기구를 강화함으로써 각종 질환에 대응하고자 하는 연구에 많은 관심이 집중 되고 있는 추세이다. 예로부터 자연계에 존재하는 여러 식물체에는 인간의 노화를 예방하는 활성성분이 존재한다고 전해져 왔고 현재까지도 건강유지의 목적으로 여전히 이를 사용하고 있다. 아울러 새로운 생리활성물질 개발의 최근 동향은 합성 의약품의 심각한 부작용 등으로 인하여 인공 합성물질보다 천연물질에 더욱 많은 비중을 두고 있고, 특히 동양의 전통적인 천연물에 대해서 현대의 생명과학기술을 활용한 광범위한 연구가 주목받고 있다.As a result, a great deal of attention has been focused on researches to cope with various diseases by strengthening the biological defense mechanism inherent in humans by using the active ingredients present in food materials. Many plants in nature have long been known to contain active ingredients that prevent human aging and are still used for health maintenance purposes. In addition, recent trends in the development of new bioactive substances have placed more weight on natural materials than artificial synthetic materials due to the serious side effects of synthetic medicines. It is attracting attention.

이러한 동향을 바탕으로 특히 천연식품인 상황버섯은 소나무 비늘버섯과의 진흙버섯(Phellinus)에 속하는 흰색의 무후균이며 뽕나무와 활엽수 줄기에 자생하고 목질진흙버섯(Phellinus linteus)이라고 하며 중국, 캄보디아, 일본 등에서 자생 또는 재배하고 있다.Based on these trends, natural mushrooms, especially natural mushrooms, are white fungi belonging to the Phellinus family of pine scaly mushrooms, which grow on mulberry and broad-leaved stems and are called Phellinus linteus . It grows or grows on the back.

상황버섯(phellinus linteus)은 당질, 단백질, 비타민, 무기질과 같은 영양소를 골고루 함유하고 있으며 상황버섯의 열수 추출물은 B 임파구 세포기능을 증가시키고 상황버섯에서 추출된 다당체가 체액성 및 세포성 면역반응 항진시킨다는 연구보고도 있어 민간에서 많이 사용되고 있다. 또한 최근 상황버섯은 항암활성, 면역증강효과 등의 약리 효과 때문에 최근에는 건강식품 및 의약품 소재로 많이 이용되고 있고 인공재배 상황버섯과 자연 상황버섯의 자실체열수추출 다당체의 항보체 활성이 비슷하여 면역활성 측면으로는 인공 상황버섯의 이용 가능성이 증가하고 있다. Phellinus linteus contains nutrients such as sugars, proteins, vitamins and minerals. The hydrothermal extract of S. mushrooms increases B lymphocyte cell function, and the polysaccharides extracted from S. mushrooms are used for humoral and cellular immune responses. There is also a research report saying that it is used a lot in the private sector. In addition, recently, situation mushrooms have been widely used as health foods and medicines because of their pharmacological effects such as anticancer activity and immune enhancing effect. On the side, the availability of artificial situation mushrooms is increasing.

본 발명은 건강식품으로 널리 알려진 상황버섯을 그 기능성을 그대로 보존하면서도 쉽게 섭취할 수 있도록 과립차로 제조하는 방법을 제공하는데 기술적 과제가 있다.The present invention has a technical problem to provide a method for producing granular tea so that it can be easily ingested while maintaining the functionality of the situation mushrooms widely known as health food.

상기한 기술적 과제를 해결하기 위해 본 발명은, (a)상황버섯을 열수추출하고 농축하는 단계; (b)작약, 당귀, 숙지황, 영지, 결명자, 생강, 천궁, 대추, 계피, 감초를 혼합한 생약재 혼합물을 열수추출하고 농축하는 단계; (c)상기 (a)단계의 상황버섯 농축액과 (b)단계의 생약재 농축액을 살균하는 단계; (d)상기 (c)단계를 통해 살균된 농축액에 대해 적정pH, 미생물 및 세균 검사를 실시하는 단계; (e)상기 (d)단계의 검사결과에 따라 일정 조건을 만족시키는 농축액과, 함수결정포도당 및 유당을 포함하는 첨가제 혼합물을 혼합하여 반죽하는 단계; (f)과립기를 이용하여 상기 (e)단계의 반죽을 과립화하는 단계; (g)건조기를 이용하여 상기 (f)단계의 과립을 건조하는 단계;로 이루어지는 것을 특징으로 하는 상황버섯을 활용한 기능성 과립차 제조방법을 제공한다.In order to solve the above technical problem, the present invention, (a) hot water extracting and concentrating a situation mushroom; (b) hydrothermally extracting and concentrating the herbal medicine mixture of peony, Angelica, Sukjihwang, Ganoderma lucidum, Cultivator, Ginger, Cheongung, Jujube, Cinnamon and Licorice; (c) sterilizing the situation mushroom concentrate of step (a) and the herbal medicine concentrate of step (b); (d) performing titration pH, microbial and bacterial tests on the concentrate sterilized through step (c); (e) mixing and kneading a concentrated solution satisfying a predetermined condition according to the test result of step (d) and an additive mixture including hydrous crystalline glucose and lactose; (f) granulating the dough of step (e) using a granulator; (g) using the dryer to dry the granules of the step (f); provides a method for producing functional granular tea utilizing the situation mushroom, characterized in that consisting of.

본 발명에 따르면, 상황버섯과 각종 생약재의 함유한 유효성분을 과립차 형태로 손쉽게 섭취할 수 있다. 특히 본 발명에 따른 과립차는 항산화와 항비만에 유효한 효과를 나타나는 것으로 확인되었다. According to the present invention, the effective ingredient containing the situation mushroom and various herbal medicines can be easily ingested in the form of granular tea. In particular, the granule tea according to the present invention was found to have an effective effect on antioxidant and anti-obesity.

본 발명은 상황버섯을 활용한 기능성 과립차 제조방법에 관한 것으로, 기능성을 그대로 보존하면서 상황버섯 추출물을 생약재 추출물과 함께 과립차로 제조한다는데 특징이 있다. 다시 말해 본 발명은, (a)상황버섯을 열수추출하고 농축하는 단계; (b)작약, 당귀, 숙지황, 영지, 결명자, 생강, 천궁, 대추, 계피, 감초를 혼합한 생약재 혼합물을 열수추출하고 농축하는 단계; (c)상기 (a)단계의 상황버섯 농축액과 (b)단계의 생약재 농축액을 살균하는 단계; (d)상기 (c)단계를 통해 살균된 농축액에 대해 적정pH, 미생물 및 세균 검사를 실시하는 단계; (e)상기 (d)단계의 검사결과에 따라 일정 조건을 만족시키는 농축액과, 함수결정포도당 및 유당을 포함하는 첨가제 혼합물을 혼합하여 반죽하는 단계; (f)과립기를 이용하여 상기 (e)단계의 반죽을 과립화하는 단계; (g)건조기를 이용하여 상기 (f)단계의 과립을 건조하는 단계;로 이루어지는 것을 특징으로 한다.The present invention relates to a method for producing functional granular tea using a situation mushroom, which is characterized in that the preparation of the situation mushroom extract with granule tea together with the herbal extract while preserving the functionality. In other words, the present invention comprises the steps of: (a) hydrothermal extraction and concentration of the mushroom; (b) hydrothermally extracting and concentrating the herbal medicine mixture of peony, Angelica, Sukjihwang, Ganoderma lucidum, Cultivator, Ginger, Cheongung, Jujube, Cinnamon and Licorice; (c) sterilizing the situation mushroom concentrate of step (a) and the herbal medicine concentrate of step (b); (d) performing titration pH, microbial and bacterial tests on the concentrate sterilized through step (c); (e) mixing and kneading a concentrated solution satisfying a predetermined condition according to the test result of step (d) and an additive mixture including hydrous crystalline glucose and lactose; (f) granulating the dough of step (e) using a granulator; (g) drying the granules of step (f) using a dryer.

상기 (b)단계의 생약재들은 본 발명에서 추구하는 기능성(항노화, 항산화, 항비만)과 과립차로서 기호성(맞, 색 등)을 충족시키기 위해 채택되었다. 특히, 본 발명은 상기 (b)단계에서 생약재들을 작약 14~19중량%, 당귀 8~13중량%, 숙지황 14~19중량%, 영지 8~12중량%, 결명자 9~13중량%, 생강 6~8중량%, 천궁 9~13중량%, 대추 8~13중량%, 계피 4~6중량%, 감초 4~6중량%로 혼합할 것을 제안하는데, 이러한 조성범위는 상기 생약재들의 기능성과 기호성을 고려하여 최적화한 범위이다.The herbal medicines of step (b) have been adopted to satisfy the functionality (anti-aging, antioxidant, anti-obesity) and palatability (correction, color, etc.) pursued in the present invention. In particular, the present invention in the step (b) peony 14-19% by weight, Angelica 8-13% by weight, 14-19% by weight of Sukji, 8-12% by weight, Ganoderma lucidum 9-13% by weight, ginger 6 ~ 8% by weight, 9-13% by weight, jujube 8-13%, cinnamon 4-6%, licorice 4-6% by weight, the composition range of the herbal properties and palatability It is an optimized range in consideration.

나아가, 상기 (a)단계는 상황버섯 농축액에서 고형분이 60~80중량%가 되도록 실시하고, 상기 (b)단계는 생약재 농축액에서 고형분이 50~80중량%가 되도록 실시하고, 아울러 상기 (e)단계는 상황버섯 농축액 7~12중량%, 생약재 농축액 15~25중량%, 함수결정포도당 40~50중량%, 유당 15~25중량%, 비타민C 1~3중량%, 비타민E 0.3~0.8중량%, 홍차농축분말 0.1~2중량%, 베타카로틴 0.05~0.15중량%로 혼합하면서 실시하는 것이 바람직하다. 이러한 조성범위는 과립차로서 기능성과 기호성을 고려하여 최적화한 범위이다.Further, the step (a) is carried out so that the solid content is 60 to 80% by weight in the situation mushroom concentrate, the step (b) is carried out so that the solid content is 50 to 80% by weight in the herbal medicine concentrate, and (e) Steps include 7 ~ 12% by weight of mushroom concentrate, 15 ~ 25% by weight herbal extract, 40 ~ 50% by weight hydrous sugar, 15 ~ 25% by weight lactose, 1 ~ 3% by weight vitamin C, 0.3 ~ 0.8% by weight vitamin E It is preferable to carry out while mixing with black tea concentrate powder 0.1-2 weight%, and beta-carotene 0.05-0.15 weight%. This composition range is optimized range in consideration of functionality and palatability as granule tea.

이하, 실시예 및 실험예에 따라 본 발명을 상세히 설명한다. 다만, 하기의 실시예와 실험예는 본 발명의 이해를 돕기 위하여 제시한 것일 뿐, 본 발명이 이에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail according to Examples and Experimental Examples. However, the following Examples and Experimental Examples are only presented to aid the understanding of the present invention, but the present invention is not limited thereto.

[실시예1] 과립차 제조 Example 1 Preparation of Granular Tea

(1)재료 준비(1) material preparation

상황버섯(국내산), 생약재(작약 16중량%, 당귀 10중량%, 숙지황 16중량%, 영지 9중량%, 결명자 11중량%, 생강 7중량%, 천궁 11중량%, 대추 10중량%(이상 국내산), 계피 5중량%, 감초 5중량/%(이상 수입산))를 준비하고, 각 재료들에 이물혼합여부를 확인한다. 생약재는 세척한다.Situation mushroom (domestic), herbal medicine (16% by weight, Angelica 10% by weight, 16% by weight of Sookji sulfur, 9% by weight of ganoderma lucidum, 11% by weight, 7% by weight of ginger, 11% by weight of cheonggung, jujube 10% by weight (more than domestic) ), 5% by weight cinnamon, 5% by weight licorice (more than imported)), and check whether each material is mixed. The herbal medicines are washed.

(2)추출(2) extraction

상황버섯에 원료무게 대비 20배의 물을 가하여 Extractor를 이용하여 95℃에 서 12시간 1차 추출한 후 여과하고, 여과하여 남은 재료에 다시 원료무게 대비 5배의 물을 가하여 Extractor를 이용하여 95℃에서 12시간 2차 추출한 후 여과한다.After extracting 20 times of water compared to the weight of raw materials to the situation mushroom, extract it firstly at 95 ℃ for 12 hours using an extractor, filter it, and add 5 times more water than the weight of the raw materials to the filtered material and then extract it to 95 ℃ using the extractor. The mixture was extracted for 2 hours at 12 hours and filtered.

생약재 혼합물도 같은 방법으로 추출하고 여과한다.The herbal mixture is also extracted and filtered in the same way.

(3)농축(3) concentration

1차 추출 여과액과 2차 추출 여과액을 혼합하고 Concentrator를 이용하여 농축하고 고형분 검사를 행한다. 상황버섯 추출물의 농축액은 고형분 함량이 70중량%이고, 생약재 추출물의 농축액은 고형분 함량이 65중량%로 확인되었다. The primary and secondary extract filtrates are mixed, concentrated using a concentrator and tested for solids. The concentration of the mushroom extract was 70% by weight solids, the concentration of the herbal extracts was confirmed to be 65% by weight solids.

(4)살균(4) sterilization

농축액을 95℃에서 살균한다. The concentrate is sterilized at 95 ° C.

(5)검사(5) inspection

적정pH와 미생물 및 세균 검사를 행한다.Titration and microbial and bacterial tests are performed.

(6)원료배합(6) Raw material blending

검사결과 기준을 통과한 농축액과, 함수결정포도당 및 유당을 포함하는 첨가제를 혼합하여 반죽한다. 첨가제로는 함수결정포도당(국산)과 유당(미국산) 외에 비타민C(수입산), 비타민E(유럽), 홍차농축분말(독일), 베타카로틴(수입)를 이용하였으며, 이들 첨가제를 이용한 반죽은 상황버섯 농축액 10중량%, 생약재 농축액 20중량%, 함수결정포도당 46.4중량%, 유당 20중량%, 비타민C 2중량%, 비타민E 0.5중량%, 홍차농축분말 1중량%, 베타카로틴 0.1중량%로 실시하였다. .Knead the mixture that passed the test result and the additive containing hydrous glucose and lactose. As additives, we used vitamin C (imported), vitamin E (Europe), black tea concentrate (Germany), and beta-carotene (imported) as well as hydrous glucose (domestic) and lactose (US). 10% by weight of mushroom concentrate, 20% by weight of herbal medicine concentrate, 46.4% by weight of sugar crystals, 20% by weight of lactose, 2% by weight of vitamin C, 0.5% by weight of vitamin E, 1% by weight of black tea concentrate powder, 0.1% by weight of beta carotene It was. .

(7)성형(7) molding

반죽 과립기를 이용하여 과립화한다.Granulate using a dough granulator.

(8)건조(8) drying

건조기를 이용하여 건조한다. 이로써 과립차가 제조되었다.Dry using a dryer. This produced granular tea.

[실험예1] 과립차의 기능성 실험Experimental Example 1 Functional Experiment of Granular Tea

(1)동물사육 및 식이(1) animal breeding and diet

식이 groups은 정상군(N))과 고지방·고콜레스테롤 식이 실험군으로 나눈 후 고지방·고콜레스테롤 실험군을 다시 상기 실시예1에 따라 제조된 과립차의 공급수준에 따라 고지방·고콜레스테롤군(HF), 0.3g/ℓ을 공급한 Tea1군과 0.6g/ℓ를 공급한 Tea2군으로 각각 10마리씩 총 4군으로 나누어 4주간 사육하였다. The diet groups were divided into the normal group (N)) and the high fat and high cholesterol dietary test group, and then the high fat and high cholesterol test group was re-divided according to the supply level of the granular tea prepared according to Example 1 above. Tea1 group fed g / l and Tea2 group fed 0.6g / l were divided into four groups of 10 animals each for 4 weeks.

그룹별 식이(g/㎏ diet)Group Diet (g / ㎏ diet) NN HFHF Tea1Tea1 Tea2Tea2 Corn starch
Casein
Sucrose
Corn oil
Mineral mixture2)
Vitamin mixture3)
Cellulose
Corn starch
Casein
Sucrose
Corn oil
Mineral mixture 2)
Vitamin mixture 3)
Cellulose
539
200
100
60
35
10
50
539
200
100
60
35
10
50
429
200
100
60
35
10
50
429
200
100
60
35
10
50
429
200
100
60
35
10
50
429
200
100
60
35
10
50
429
200
100
60
35
10
50
429
200
100
60
35
10
50
DL-methionine
Choline Chloride
Lard
Cholesterol
Phellinus linteus4)
DL-methionine
Choline chloride
Lard
Cholesterol
Phellinus linteus 4)
3
3
-
-
-
3
3
-
-
-
3
3
100
10
-
3
3
100
10
-
3
3
100
10
0.3g/ℓ
3
3
100
10
0.3g / ℓ
3
3
100
10
0.6g/ℓ
3
3
100
10
0.6g / ℓ
TotalTotal 10001000 10001000 10001000 10001000 1)N: Normal diet
HF: High fatHigh cholesterol diet
Tea1: High fatHigh cholesterol diet + Phellinus linteus tea 0.3g/l
Tea2: High fatHigh cholesterol diet + Phellinus linteus tea 0.6g/l
The diet of experiment groups were supplemented with 0.4%(4g/kg) the mixtures.
2) AIN-76 mineral mixture (g/kg mixture).
3) AIN-76 vitamin mixture (mg/kg mixture).
4) Phellinus linteus: kumhwang
1) N: Normal diet
HF: High fat High cholesterol diet
Tea1: High fatHigh cholesterol diet + Phellinus linteus tea 0.3g / l
Tea2: High fatHigh cholesterol diet + Phellinus linteus tea 0.6g / l
The diet of experiment groups were supplemented with 0.4% (4g / kg) the mixtures.
2) AIN-76 mineral mixture (g / kg mixture).
3) AIN-76 vitamin mixture (mg / kg mixture).
4) Phellinus linteus : kumhwang

(2)체중증가량, 식이섭취량, 식수섭취량 및 식이효율 관찰(2) Observation of weight gain, dietary intake, drinking water intake and dietary efficiency

상황버섯 차의 농도별 체중증가량, 식이섭취량, 식수섭취량 및 식이효율을 관찰한 결과는 하기 [표 2]와 같이 나타났다. 식이효율(food efficiency ratio, FER)은 전 체중증가량을 같은 기간동안의 식이섭취량으로 나누어 줌으로써 계산하였다. The results of observing the weight gain, dietary intake, drinking water intake and dietary efficiency by concentration of the situation mushroom tea are shown in the following [Table 2]. The food efficiency ratio (FER) was calculated by dividing the total weight gain by the food intake over the same period.

하기 [표 2]에서 보는 바와 같이 체중증가량은 정상군(N)에 비해 고지방·콜레스테롤군(HF)에서 증가되었으나 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2)에서는 감소하였으며, 식이효율도 이와 유사한 경향이었다. As shown in [Table 2] below The weight gain was increased in the high fat / cholesterol group (HF) compared to the normal group (N), but decreased in the group (Tea1, Tea2) fed the granular tea according to the present invention, the dietary efficiency was similar.

체중증가량(Body weight gains), 식이섭취량(Food intake), 식수섭취량Water intake 및 식이효율(FER)Body weight gains, food intake, water intake and FER GroupGroup Body weight gains
(g)
Body weight gains
(g)
Water intake
(g/day)
Water intake
(g / day)
Food intake
(g/day)
Food intake
(g / day)
FERFER
NN 88.67±27.22NS 88.67 ± 27.22 NS 43.57±8.067NS 43.57 ± 8.067 NS 25.26±3.47NS 25.26 ± 3.47 NS 0.564±0.05b 0.564 ± 0.05 b HFHF 126.50±24.84   126.50 ± 24.84 48.97±9.57448.97 ± 9.574 22.48±2.8622.48 ± 2.86 0.827±0.02a 0.827 ± 0.02 a Tea1Tea1 116.60±23.21    116.60 ± 23.21 48.89±16.9248.89 ± 16.92 21.20±4.5421.20 ± 4.54 0.801±0.03a 0.801 ± 0.03 a Tea2Tea2 110.41±25.09    110.41 ± 25.09 48.05±15.7348.05 ± 15.73 20.31±4.1820.31 ± 4.18 0.799±0.01a 0.799 ± 0.01 a All values are mean±SE (n=10). Those with different superscripts in the same column are significantly different at p<0.05 by Tukey's test. All values are mean ± SE (n = 10). Those with different superscripts in the same column are significantly different at p <0.05 by Tukey's test.

(2)항산화 효소 활성 관찰(2) observing antioxidant enzyme activity

1)간조직의 superoxide radical 함량1) superoxide radical content in liver tissue

간조직의 microsome에서 superoxide radical 함량을 측정한 결과는 하기 [표 3]과 같이 나타났다.The results of measuring the superoxide radical content in the microsome of liver tissue are shown in the following [Table 3].

하기 [표 3]에서 보는 바와 같이 정상군(N)에 비해 HF군에서 증가되었으나 본 발명에 따른 과립차를 공급한 모든 군(Tea1, Tea2)에서 고지방·콜레스테롤군(HF)에 비교하여 유의적으로 감소되었으며, 또한 간조직의 mitochondria에서는 본 발명에 따른 과립차를 공급한 모든 군(Tea1, Tea2)에서 고고지방·콜레스테롤군(HF)보다 감소하였으나 유의적인 차이가 없었다.As shown in the following [Table 3] Compared to the normal group (N) was increased in the HF group, but all groups fed the tea according to the present invention (Tea1, Tea2) significantly decreased compared to the high fat and cholesterol group (HF), and also in the liver tissue mitochondria In all groups (Tea1, Tea2) fed granulated tea according to the present invention, there was no significant difference, compared to the high fat / cholesterol group (HF).

간조직의 superoxide radical 함량Superoxide radical content in liver tissue GroupGroup microsomemicrosome mitochondriamitochondria (nmole/ protein/min)(nmole / protein / min) NN 6.815±0.500b 6.815 ± 0.500 b 13.68±2.408NS 13.68 ± 2.408 NS HFHF 8.599±0.645a 8.599 ± 0.645 a 17.13±4.82417.13 ± 4.824 Tea1Tea1 5.133±0.536c 5.133 ± 0.536 c 12.46±2.38312.46 ± 2.383 Tea2Tea2 4.297±0.308c 4.297 ± 0.308 c 12.88±1.27312.88 ± 1.273 Values are the means±SE(n=10). Those with different superscript letters are significantly different at p<0.05 by Tukey's test. Values are the means ± SE (n = 10). Those with different superscript letters are significantly different at p <0.05 by Tukey's test.

2)간조직 중의 항산화 효소계 관찰: SOD 및 GPx 활성 2) Observation of antioxidant enzyme system in liver tissue: SOD and GPx activity

생체 내 항산화방어기구 중 효소적 방어계의 하나로서 superoxide radical을 H2O2로 환원시킴으로써 산소 독으로부터 생체를 보호하는 SOD 활성을 간조직에서 관찰한 결과는 하기 [표 4]와 같이 나타났다. As a result of the enzymatic defense system of the in vivo antioxidant defense mechanism, the result of observing the SOD activity in the liver tissue to protect the living body from oxygen poison by reducing superoxide radical to H 2 O 2 is shown in Table 4 below.

하기 [표 4]에서 보는 바와 같이 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2)이 고지방·콜레스테롤군(HF)에 비해 다소 증가하는 경향을 보였다. 또한, selenium을 함유하는 항산화 효소로 과산화물을 제거함으로써 세포막의 손실을 방어하는 GSHpx의 활성을 관찰한 결과는 고지방·콜레스테롤군(HF)에 비해 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2)에서 유의적으로 증가하는 경향을 보였다.As shown in the following [Table 4], the group fed with the tea according to the present invention (Tea1, Tea2) showed a tendency to increase slightly compared to the high fat-cholesterol group (HF). In addition, the results of observing the activity of GSHpx to protect the loss of cell membranes by removing peroxides with antioxidant enzymes containing selenium were compared with the high fat and cholesterol groups (HF). Showed a tendency to increase significantly.

SOD 및 GPx 활성 SOD and GPx Active GroupsGroups SODSOD GSH-pxGSH-px (unit/ ㎎ protein/ min)(unit / mg protein / min) (nmol NADPH/ min
/ ㎎ protein)
(nmol NADPH / min
/ Mg protein)
NN 1.244±0.082b 1.244 ± 0.082 b 394.49±40.71bc 394.49 ± 40.71 bc HFHF 1.401±0.079ab 1.401 ± 0.079 ab 284.08±59.26c 284.08 ± 59.26 c Tea1Tea1 1.469±0.070a 1.469 ± 0.070 a 466.52±66.29ab 466.52 ± 66.29 ab Tea2Tea2 1.558±0.100a 1.558 ± 0.100 a 502.92±23.72a 502.92 ± 23.72 a All values are mean±SE(n=10). Those with different superscripts in the same column are significantly different at p<0.05 by Tukey's test. All values are mean ± SE (n = 10). Those with different superscripts in the same column are significantly different at p <0.05 by Tukey's test.

(3)산화적 손상 관찰 : 간조직 중의 과산화지질(TBARS)함량(3) Oxidative damage observation : TBARS content in liver tissue

체내 지질과산화의 지표가 되는 지질과산화물가를 간조직에서 관찰한 결과는 하기 [표 5]와 같이 나타났다.The results of observing the lipid peroxide value, which is an indicator of lipid peroxidation in the liver, are shown in the following [Table 5].

하기 [표 5]에서 보는 바와 같이 고지방·콜레스테롤군(HF)에 비해 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2)에서 감소하였다. 특히 본 발명에 따른 과립차의 농도를 높게 준 군(Tea2)은 정상군(N) 수준으로 감소하였다. 혈장에서 측정한 결과 또한 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2) 모두에서 감소하였고, 특히 본 발명에 따른 과립차의 농도를 높게 한 Tea2군은 정상군(N) 수준으로 감소하였다.As shown in the following [Table 5], compared to the high fat cholesterol group (HF) was reduced in the group (Tea1, Tea2) fed granule tea according to the present invention. In particular, the group (Tea2) with high concentration of granular tea according to the present invention was reduced to the normal group (N) level. As a result of the measurement in plasma, both the groups supplied with the tea according to the present invention (Tea1, Tea2) were decreased, and in particular, the Tea2 group with the high concentration of the granule tea according to the present invention was reduced to the normal group (N) level.

간조직 중의 과산화지질(TBARS)함량Lipid peroxide (TBARS) content in liver tissue GroupsGroups LiverLiver PlasmaPlasma (nmole/g tissue)(nmole / g tissue) (nmol/㎖)(nmol / ml) NN 13.20±1.58c 13.20 ± 1.58 c 3.64±0.09b 3.64 ± 0.09 b HFHF 26.03±2.25a 26.03 ± 2.25 a 6.11±0.79a 6.11 ± 0.79 a Tea1Tea1 21.61±2.28ab 21.61 ± 2.28 ab 4.76±1.19ab 4.76 ± 1.19 ab Tea2Tea2 17.86±2.85bc 17.86 ± 2.85 bc 4.14±0.54b 4.14 ± 0.54 b Values are the means±SE(n=10). Those with different superscript letters are significantly different at p<0.05 by Tukey's test.  Values are the means ± SE (n = 10). Those with different superscript letters are significantly different at p <0.05 by Tukey's test.

(4)항비만 효과 관찰(4) observation of anti-obesity effect

1)혈당(Blood glucose) 관찰1) Blood glucose observation

혈당을 관찰한 결과는 하기 [표 6]과 같이 나타났다.The result of observing blood glucose was shown in the following [Table 6].

하기 [표 6]에서 보는 바와 같이 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2)에서 정상군(N) 수준으로 감소하였다.As shown in Table 6, the granule tea according to the present invention was reduced to the normal group (N) level in the fed group (Tea1, Tea2).

혈당Blood sugar GroupsGroups Blood glucoseBlood glucose (mg/dl)(mg / dl) NN 152.4 ± 9.853b 152.4 ± 9.853 b HFHF 191.4 ± 6.750a 191.4 ± 6.750 a Tea1Tea1 164.5 ± 9.711b 164.5 ± 9.711 b Tea2Tea2 165.1 ± 3.792b 165.1 ± 3.792 b All values are mean±SE (n=10). Those with different superscripts in the same column are significantly different at p<0.05 by Tukey's test. All values are mean ± SE (n = 10). Those with different superscripts in the same column are significantly different at p <0.05 by Tukey's test.

2)중성지방 관찰 : triglyceride , total-cholesterol2) Triglyceride observation: triglyceride, total-cholesterol

심혈관질환에서는 혈중 콜레스테롤 및 지질농도의 증가를 흔히 관찰할 수 있는데, 이러한 상태를 반영하는 지표인 triglyceride 및 total-cholesterol 함량의 결과는 하기 [표 7]과 같이 나타났다.In cardiovascular disease, an increase in blood cholesterol and lipid concentrations can often be observed, and the results of triglyceride and total-cholesterol contents, which reflect these conditions, are shown in Table 7 below.

하기 [표 7]에서 보는 바와 같이 혈청 중의 중성지방 농도는 본 발명에 따른 과립차를 공급한 모든 군(Tea1, Tea2)이 정상군(N) 수준으로 감소되었다. 또한 총콜레스테롤 농도는 정상군(N)에 비해 고지방·콜레스테롤군(HF)에서 증가되었고, 고지방·콜레스테롤군(HF)에 비해 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2) 모두에서 유의적으로 감소되었으며, 특히 본 발명에 따른 과립차의 농도를 높게 한 Tea2군은 정상군(N) 수준으로 감소하였다. As shown in the following [Table 7], the triglyceride concentration in serum was reduced to the normal group (N) level in all groups (Tea1, Tea2) fed granulated tea according to the present invention. In addition, the total cholesterol concentration was increased in the high fat / cholesterol group (HF) compared to the normal group (N), and significantly higher in both the group fed the granulated tea according to the present invention (Tea1, Tea2) compared to the high fat / cholesterol group (HF). In particular, the Tea2 group having a high concentration of granular tea according to the present invention was reduced to the normal group (N) level.

triglyceride , total-cholesteroltriglyceride, total-cholesterol Group Group Triglyceride    Triglyceride Total cholesterolTotal cholesterol (mg/dl)(mg / dl) NN 70.03±9.42b 70.03 ± 9.42 b 72.32±3.79c 72.32 ± 3.79 c HFHF 95.17±8.50a 95.17 ± 8.50 a 119.14±8.83a 119.14 ± 8.83 a Tea1Tea1 79.09±7.89ab 79.09 ± 7.89 ab 86.01±5.17b 86.01 ± 5.17 b Tea2Tea2 69.97±7.32b 69.97 ± 7.32 b 78.76±4.67bc 78.76 ± 4.67 bc All values are mean±SE (n=10). Values within a column with different superscripts are significantly different among groups at p<0.05 by Tukey's test All values are mean ± SE (n = 10). Values within a column with different superscripts are significantly different among groups at p <0.05 by Tukey's test

(5)생체 무독성 규명: GOT, GPT의 활성관찰(5) Identification of biological nontoxicity: observation of activity of GOT and GPT

간의 괴사를 반영하는 GOT와 간조직의 비대화 및 간의 상태를 반영하는 GPT의 활성을 측정한 결과는 하기 [표 8]과 같이 나타났다.GOT reflecting liver necrosis and hypertrophy of liver tissue and the results of measuring the activity of GPT reflecting the status of the liver were shown in Table 8 below.

하기 [표 8]에서 보는 바와 같이 GOT 활성은 고지방·콜레스테롤군(HF)에 비해 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2)에서 정상군(N) 수준으로 감소하였다. GPT 활성 또한 고지방·콜레스테롤군(HF)에 비해 본 발명에 따른 과립차를 공급한 군(Tea1, Tea2)에서 감소하였고, 특히 본 발명에 따른 과립차의 농도를 높게 한 Tea2군은 정상군(N) 수준으로 감소하였다.As shown in the following [Table 8], the GOT activity was reduced to the normal group (N) level in the group fed with the tea according to the present invention (Tea1, Tea2) compared to the high fat cholesterol group (HF). GPT activity was also decreased in the high fat / cholesterol group (HF) group (Tea1, Tea2) fed granule tea according to the present invention, especially Tea2 group with a high concentration of granular tea according to the present invention (N) level Decreased.

GOT, GPT 활성GOT, GPT active GroupsGroups GOTGOT GPTGPT (karmen/㎖)(karmen / ml) (karmen/㎖)(karmen / ml) NN 28.50±4.60b 28.50 ± 4.60 b 12.00±2.97c 12.00 ± 2.97 c HFHF 40.50±4.72a 40.50 ± 4.72 a 28.00±3.77a 28.00 ± 3.77 a Tea1  Tea1 32.25±4.43ab 32.25 ± 4.43 ab 19.67±2.31b 19.67 ± 2.31 b Tea2  Tea2 30.60±4.16ab 30.60 ± 4.16 ab 15.00±2.83bc 15.00 ± 2.83 bc All values are mean±SE (n=10). Values within a column with different superscripts are significantly different among groups at p<0.05 by Tukey's test All values are mean ± SE (n = 10). Values within a column with different superscripts are significantly different among groups at p <0.05 by Tukey's test

Claims (3)

(a)상황버섯을 열수추출하고 농축하는 단계;(a) hydrothermal extraction and concentration of the situation mushroom; (b)작약, 당귀, 숙지황, 영지, 결명자, 생강, 천궁, 대추, 계피, 감초를 혼합한 생약재 혼합물을 열수추출하고 농축하는 단계;(b) hydrothermally extracting and concentrating the herbal medicine mixture of peony, Angelica, Sukjihwang, Ganoderma lucidum, Cultivator, Ginger, Cheongung, Jujube, Cinnamon and Licorice; (c)상기 (a)단계의 상황버섯 농축액과 (b)단계의 생약재 농축액을 살균하는 단계;(c) sterilizing the situation mushroom concentrate of step (a) and the herbal medicine concentrate of step (b); (d)상기 (c)단계를 통해 살균된 농축액에 대해 적정pH, 미생물 및 세균 검사를 실시하는 단계;(d) performing titration pH, microbial and bacterial tests on the concentrate sterilized through step (c); (e)상기 (d)단계의 검사결과에 따라 일정 조건을 만족시키는 농축액과, 함수결정포도당 및 유당을 포함하는 첨가제를 혼합하여 반죽하는 단계;(e) mixing and kneading a concentrated solution that satisfies a predetermined condition according to the test result of step (d) and an additive including hydrous crystalline glucose and lactose; (f)과립기를 이용하여 상기 (e)단계의 반죽을 과립화하는 단계;(f) granulating the dough of step (e) using a granulator; (g)건조기를 이용하여 상기 (f)단계의 과립을 건조하는 단계;(g) drying the granules of step (f) using a dryer; 로 이루어지는 것을 특징으로 하는 상황버섯을 활용한 기능성 과립차 제조방법.Functional granular tea manufacturing method using the situation mushroom, characterized in that consisting of. 제1항에서,In claim 1, 상기 (b)단계는 작약 14~19중량%, 당귀 8~13중량%, 숙지황 14~19중량%, 영지 8~12중량%, 결명자 9~13중량%, 생강 6~8중량%, 천궁 9~13중량%, 대추 8~13중량%, 계피 4~6중량%, 감초 4~6중량%로 혼합하면서 이루어지는 것을 특징으로 하는 상황버섯을 활용한 기능성 과립차 제조방법.Step (b) is peony 14-19% by weight, Angelica 8-13% by weight, Sukjihwang 14-19% by weight, Ganoderma lucidum 8-12% by weight, 9-13% by weight, 6-8% by weight ginger, 9 Method for producing a functional granular tea using a situation mushroom, characterized in that the mixture consisting of ~ 13% by weight, jujube 8-13% by weight, cinnamon 4-6% by weight, licorice 4-6% by weight. 제1항 또는 제2항에서,The method of claim 1 or 2, 상기 (a)단계는 고형분이 60~80중량%가 되도록 이루어지며,The step (a) is made so that the solid content is 60 to 80% by weight, 상기 (b)단계는 고형분이 50~80중량%가 되도록 이루어지며,The step (b) is made so that the solid content is 50 to 80% by weight, 상기 (e)단계는 상황버섯 농축액 7~12중량%, 생약재 농축액 15~25중량%, 함수결정포도당 40~50중량%, 유당 15~25중량%, 비타민C 1~3중량%, 비타민E 0.3~0.8중량%, 홍차농축분말 0.1~2중량%, 베타카로틴 0.05~0.15중량%로 혼합하면서 이루어지는 것을 특징으로 하는 상황버섯을 활용한 기능성 과립차 제조방법.The step (e) is 7 ~ 12% by weight of concentrated mushroom concentrate, 15 ~ 25% by weight of herbal medicine concentrate, 40-50% by weight of hydrous crystalline glucose, 15-25% by weight lactose, 1 to 3% by weight of vitamin C, vitamin E 0.3 ~ 0.8% by weight, black tea concentrated powder 0.1 ~ 2% by weight, beta carotene 0.05 ~ 0.15% by weight of the functional granular tea production method using the situation mushroom, characterized in that the mixture is made.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102302068A (en) * 2011-09-21 2012-01-04 湖南中医药大学 Tea beverage for preventing and treating metabolic syndrome
CN102813024A (en) * 2011-06-09 2012-12-12 福建农大菌草技术开发公司 Tea blend with grass-cultivated ganoderma lucidum serving as matrix and added with white tea
KR20190057539A (en) * 2017-11-20 2019-05-29 한경대학교 산학협력단 Antioxidant activity intensified granule tea with composition of fruiting body of Hericium erinaceum and hot water extract of Phellinus linteus

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WO2016035915A1 (en) * 2014-09-05 2016-03-10 박명희 Method for making phellinus linteus tea
KR101627837B1 (en) * 2015-12-30 2016-06-07 전성보 Health supplement food comprising horse bone extracts as main ingredients and sea horse and manufacturing method for the same

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KR100828589B1 (en) * 2006-07-13 2008-05-09 노기철 Method of manufacturing granular tea containing agaricus brazei and the granular tea manufactured thereby

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102813024A (en) * 2011-06-09 2012-12-12 福建农大菌草技术开发公司 Tea blend with grass-cultivated ganoderma lucidum serving as matrix and added with white tea
CN102302068A (en) * 2011-09-21 2012-01-04 湖南中医药大学 Tea beverage for preventing and treating metabolic syndrome
KR20190057539A (en) * 2017-11-20 2019-05-29 한경대학교 산학협력단 Antioxidant activity intensified granule tea with composition of fruiting body of Hericium erinaceum and hot water extract of Phellinus linteus

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