Summary of the invention
The purpose of this invention is to provide a kind of can be antibiotic, antiviral, kill and prevent and treat agricultural pest, promote the active chemical components composition that from the pure natural plant medicine, refines of crop growth, effect significantly, low toxicity, drug residue free, non-environmental-pollution, nuisanceless, to people, animal, birds safety, can not cause that microorganism develops immunity to drugs to it, a kind of natural biological combination prevented and treated agricultural pest and preparation method thereof that is used to that has antibacterial sterilization concurrently.
Purpose of the present invention is achieved through the following technical solutions:
A kind of natural biological combination that is used to prevent and treat agricultural pest is characterized in that being made up of the raw material and the auxiliary material of following weight parts:
Elscholtiza elaboration 20-50 part
Organic solvent low-carbon alcohols 60-80 part
Synergist 4-8 part
Surfactant 1-3 part
900 parts of deionized waters
The effective active composition of described elscholtiza elaboration is the terpene phenolic compound, and the 2-4 kind compatibility by in Mosla chinensis, wild marjoram and wild fragrant weak, banksia rose Ru, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth), the Elsholtzia densa plant obtains through extraction of organic solvent sonic oscillation or diacolation.
Also refine in the effective active composition of described elscholtiza elaboration flavonoids is arranged, organic phenolic acid, and tannin, chlorophyll compounds, they when extracting elaboration and terpene phenol together from bulk drug, propose.
The effective active components of described elscholtiza elaboration is made by the following weight parts proportion raw material:
Mosla chinensis 10-15 part
Wild marjoram 6-10 part
Elsholtzia densa 4-8 part
4 parts of Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth)s
Raw material and weight portion proportioning that described elscholtiza elaboration makes effective active components are:
Mosla chinensis 10-20 part
Wild marjoram 10-20 part
Described synergist is selected from a kind of in the following compounds: Halomine, fumaric acid, glycol ether, acrylic acid.
Described surfactant comprises a kind of following material that is selected from, aliphatic acid polyethenoxy ether, APEO, aralkyl phenolic ether, sorbitan fatty acid ester, polyoxyethylene carboxylate, alkyl amine oxide, alkylsulfonate, alkylbenzenesulfonate, alkylnaphthalene sulfonate.
Preparation as follows:
A, preparation elscholtiza elaboration
By the selected Mosla chinensis of the described weight portion proportioning of claim 4, wild marjoram, Elsholtzia densa, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth);
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration is that the organic solvent low-carbon alcohols of 40-95% extracts through sonic oscillation, proportioning is: four plant species medicines are composition: low-carbon alcohols=1:6-10 by weight, concentrating back gained medicinal extract proportion is 1.0-1.4, medicinal extract is put into centrifugal vacuum desiccator, carried out drying 680-700mmHg and 40-50 ℃ and get dry extract. place cracker to pulverize dried cream, cross the 80-120 mesh sieve, promptly make the elscholtiza elaboration.
B, with the elscholtiza elaboration that makes proportioning by the described weight portion of claim 1, earlier the elscholtiza elaboration is added in the organic solvent, after 20 minutes, add the abundant stirring and dissolving of synergist, surfactant and deionized water more respectively through ultrasonic processing, filtering, packing are promptly made.
The present invention has following beneficial effect:
The present invention is the natural biological agricultural chemicals of a kind of safety, non-environmental-pollution, drug residue free, used primary raw material is an effective active composition in two kinds of generally acknowledged labiates of countries in the world, be aided with additive, surfactant, organic solvent, through science compatibility, reasonable combination, adopt vanguard technology to make environment friendly agricultural.Two kinds of labiate main chemical compositions are terpene phenol and precursor, flavonoids and organic phenolic acid etc.With this two plant species such as Mosla chinensis, wild marjoram and the combination of other elscholtiza class plant science, through extraction of organic solvent sonic oscillation or diacolation, obtain the effective active composition as host, do not have the reasonable compatibility and the homogeneous such as synergist, surfactant of taboo again with the 2-3 kind, each composition can play the enhancing synergistic effect in the prepared product.
Effective active composition of the present invention not only keeps natural assembled state but also have very strong surface-active and fat-soluble, can see through pathogeny microbial cell film rapidly, makes the generation sex change of pathogeny structural protein and solidifies, thereby cause the H of pathogenic microorganisms
+And K
+Permeability, ion component be dispersed and cause keyly in the cell occurring that process is obstructed and the cell component seepage, thus dehydration and disequilibrium causes the death of harmful germ.Bioactive ingredients such as terpene phenol, flavonoids and organic phenolic acid can quicken Normocellular turnover rate in addition, significantly reduce the infection of pathogeny body to cell in the plant, improve the ability of digesting and assimilating to nutriment, thereby have more improved the plant growing performance.Provide a kind of and can prevent and treat diseases and pests of agronomic crop effectively, the multifunctional green biopesticide that promotes plant growing again, crop yield is increased.This biopesticide has efficiently, low toxicity, drug residue free, non-environmental-pollution, nuisanceless, do not produce advantages such as poisoning to people, animal, birds safety, to plant, show that through pharmacology, toxicity and control agricultural pest test this product has splendid antibacterial sterilization, prevents and treats agricultural pest and makes short the giving birth to of crops urge multiple functions such as long.
Main natural drug among the present invention (as Mosla chinensis and wild marjoram) can be a spontaneous growth, also can be tame plant shoot branch.
One, elscholtiza elaboration of the present invention (low-carbon alcohols extract) suppresses in the cell pipe and the test of kill virus
1. primary raw material:
A, elscholtiza elaboration (Longnan Prefecture, Gansu Province buying, raw material obtains active site after the plant screening).
B, herpes simplex virus (are called for short HSV
1), Beijing institute of Biological Products provides.
C, Coxsackie virus (are called for short Cox B
1IVV), the Kunming institute of Biological Products provides.
D, A-549 passage cell strain, the Lanzhou institute of Biological Products provides.
E, baking oven, culture plate, medium and box lumber etc.
2, method step: the microneutralization test method that adopts fixed virus dilution medicine:
A, medicine titration: obtain the medicine half lethal dose, calculate safe and effective amount.
B, titration of virus: obtain viral half lethal dose (TCID
50).
C, neutralization experiment: observe the minimum dose (being effective dose) that the high dilution of medicine can suppress kill virus.
3, result:
A, medicine half lethal dose are 0.9mg/mL, the elscholtiza elaboration are described when containing 0.96mg/mL, can cause half histiocytic pathology death (being the toxicity of medicine pair cell).
B, the safe dose of elaboration in the cell pipe, by the 1/5-1/10 calculating of half lethal dose, the elscholtiza elaboration is 0.096mg/ml.
C, elaboration effective dose: when elaboration content is 0.17mg/ml, can suppress 1000 TCID
50HSV1 elaboration content when being 0.034mg/ml, can kill 100TVID
50HSV
1Suppress 1000TCID simultaneously
50HSV
1When elaboration content is 0.034mg/ml, can also suppress 100TCID
50Cox B
1IVV.
Suppress--refer to that medicine suppresses virus, the protection cell does not produce the dosage of pathology death.
Kill--refer to that medicine kills virus, the protection cell does not produce pathology death, and can continue to go down to posterity and the dosage of new pathology do not occur.
4, conclusion:
A, test tissue cell result show: dna virus be killed and be suppressed to this elaboration can not only (as HSV
1), and can suppress RNA viruses (as Cox B
1IVV), illustrate that this elaboration is the broad spectrum activity antiviral drugs.
The virus back cell that goes out when content is 17-34mg/mL can continue division and goes down to posterity but B, this elaboration safe dose are 194-96mg/Kg, illustrates that elaboration pair cell nothing itself influences, and is be a kind of safe, effective, medicine that toxicity is little.
Two, physiologically active test
(1) to the antibacterial sterilization functions of phytopathogen
Biopesticide all has significant preventive and therapeutic effect (seeing Table 1) to pepper powdery mildew, botrytis cinerea, phytophthora and cotton-wilt fusarium and tomato leaf mould
Table 1, to the antibacterial sterile test of phytopathogen
(2) control of diseases and pests of agronomic crop is tested
Diseases and pests of agronomic crop there is tangible control efficiency, tangible control efficiency (seeing Table 2) is arranged as the aphid of peach, the acarid of apple tree and the mythimna separata of cotton.
Table 2, to the control of diseases and pests of agronomic crop test
(3) elscholtiza elaboration, Mosla chinensis elaboration, phenol and carboxylic acids compare the inhibition effect of bacterium
Supply test agent: elscholtiza elaboration and Mosla chinensis elaboration, phenol, calcium propionate.
Test method: adopt the agar plate bacteriostatic method, antibacterial circle diameter unit is mm.
Various sample concentrations are respectively 250,500,800,1000ppm.
For trying bacterial classification: bacillus subtilis, staphylococcus aureus, Escherichia coli, proteus vulgaris.
The fungistatic effect of table 3, the smart powder of Mosla chinensis, the smart powder of elscholtiza, phenol, calcium propionate (MIC, ppm)
From the table as can be known, under same concentration conditions, Mosla chinensis elaboration and elscholtiza elaboration to the fungistatic effect of listed malignant bacteria in the above-mentioned table all than phenol, calcium propionate is much better than, wherein the elscholtiza elaboration is good especially.
Three, the anti-pepper powdery mildew of this biopesticide, gray mold and to the influence test of yield of hot pepper
Test site: San Ying village, street, Chengguan District Fu Long level ground, Lanzhou greenhouse by solar heat, height above sea level 2130m, Jiao Shui irrigates, and soil fertility is better, and what preceding stubble was planted is watermelon.May, powdery mildew, gray mold took place in this greenhouse capsicum simultaneously.
Reagent agent: the elscholtiza elaboration uses deionized water according to 1:100,1:200, and 1:300, A1, A2, A3, A4, A5 and 6 processing of clear water contrast are established in 1:400 and 1:500 dilution, test.
Trial crops and scope: greenhouse capsicum, kind are Gansu Province green pepper No. 2.Sub-district area 15.6m
2, sub-district randomized arrangement, 3 repetitions.
Controlling object: powdery mildew, gray mold.
Field efficacy investigation and calculating test: adopt workers and peasants---16B type knapsack hand sprayer, the complete stool even spraying is carried out with the medicament that oneself prepares in every sub-district.Spray medicine altogether 2 times, twice interval time is 8 days.Investigate diseased plant rate and disease index earlier before spraying medicine, investigated diseased plant rate and disease index in 7 days behind each medicine, investigate each sub-district disease occurrence degree, 5 samplings in every sub-district, every some fixed point investigation 6 strains, calculating diseased plant rate and disease index
Result of the test shows: at first concentration A1, A5 preventive effect have reached 100%, and secondly A3 has reached 95.73%, and A4 has reached 93.23, and A4 has reached 91.50, illustrates that elscholtiza class botanical pesticide has preferable control efficiency to pepper powdery mildew, gray mold.
Secondly growth promotion also has good effect, and results capsicum time-division sub-district is actual weighs and to measure the single fruit of capsicum long, adds up each cell production.5 kinds of concentration are all influential to yield of hot pepper.Use A1 and A3 to make yield increased increase by 42.90%, use A3, A4, A5 to compare respectively according to increasing by 8.57%, 2.87%, 14.29%.Illustrate that elscholtiza class botanical pesticide has the effect that promotes chili growth.
Four, this biopesticide greenhouse by solar heat test of pesticide effectiveness;
Experimental unit: Inst. of Vegetables, Gansu Prov. Academy of Agricultural Science
The experiment place: greenhouse by solar heat base, Liu Ba township, Yongchang County, Gansu Province experimental field is a loam.,
Experimental cultivar: capsicum variety is a Radix Polygalae Crotalarioidis; Tomato variety is a hair powder 802.
Time: in April, 2007.
Method and experimental result: this biopesticide and water are pressed 1:100,1:400,1:800 is diluted to medicine liquid spray,, 1000 times of liquid of the triazolone missible oil with 20% are contrast, and establish and do not spray medicine and make blank, sub-district area 10M
2Each handles randomized arrangement.Begin dispenser by Treatment Design in pepper powdery mildew and cladosporium leaf and fruit mould of tomato their early stage, afterwards once, amount to three times every dispenser in seven days.
According to the investigation of state of an illness grade scale, the result shows: the biopesticide that dilutes 400 times is better than 1000 times triazolone to the inhibitory or killing effect of above-mentioned two kinds of germs, can reach 88-90%.And this agricultural chemicals except that control efficiency significantly, to the crop safety, and the crop normal growth had certain facilitation.
Five, the indoor bioassay test of elscholtiza elaboration control Phytophthora capsici germ and cotton-wilt fusarium
Experiment place: Inst. of Plant Protection, Gansu Prov. Academy of Agricultural Sciences
Test objective: measure the inhibitory action of elscholtiza elaboration by indoor growth rate method, determine the valid density of this elaboration to two kinds of germs to Phytophthora capsici germ and cotton-wilt fusarium.
For trying bacterial classification: phytophthora blight of pepper; Cotton-wilt fusarium
Condition of culture: will transfer on the PDA medium for the phytophthora blight of pepper and the cotton wilt fusarium of examination, and place growth in 25 ℃ of incubators, and use after 7 days.
Test medicine: reagent agent is an elscholtiza elaboration 1
#With 2
#, be solvent with ethanol, content is 0.075g/L.Former medicine is diluted to 3 different concentration by design, determines the concentration range of medicament activity, in this scope, former medicine is diluted to 5 different concentration again and carries out the toxicity test test.Each medicament is established 5 processing, and each is handled 4 times and repeats.
Test method: with the soup of the variable concentrations that configures when the PDA medium is cooled to 45-50 ℃, ratio in soup: medium=1:10 mixes in the culture dish of pouring diameter 90mm into, with of the card punch punching of 7 days pathogen of growth with 5mm, be connected on the medium for preparing soup then respectively, being put in 25 ℃ the incubator and cultivating, is blank with the medium of adding medicine not.
Result of the test shows: in the concentration range of setting, and elscholtiza elaboration 1
#With 2
#Number medicament all has goodish inhibitory action to Phytophthora capsici germ and cotton-wilt fusarium, and along with the increase bacteriostasis rate of concentration raises 1
#Medicament reached 95-75% to the bacteriostasis rate of Phytophthora capsici germ at the 6th day, the bacteriostasis rate of cotton-wilt fusarium is reached 67.2%; 2
#Medicament reached 56.94% and 94.81% to the bacteriostasis rate of phytophthora blight of pepper and cotton-wilt fusarium respectively at the 6th day.From valid density, elscholtiza elaboration 1
#The concentration that medicament suppresses 50% growth of Phytophthora capsici germ is 3.3 * 10
-4Mg/mL, the concentration that suppresses its 90% growth reaches 5.3 * 10
-4Mg/mL, the concentration that suppresses cotton-wilt fusarium 50% and 90% growth is respectively 2.9 * 10
-4Mg/mL and 1.01 * 10
-4Mg/Ml; Elscholtiza elaboration 2
#The concentration that medicament suppresses Phytophthora capsici germ 50% and 90% growth is respectively 4 * 10
-4Mg/mL and 1.52 * 10
-3Mg/mL, the concentration that suppresses cotton-wilt fusarium 50% and 90% is respectively 1.7 * 10
-4Mg/mL and 3.5 * 10
-4Mg/mL.Show that this essence product have fungistatic effect preferably to phytophthora blight of pepper and cotton-wilt fusarium.
Six, the test of control peach aphid effect and formulation rate
Test site: the peaceful fort of the Lanzhou Anning District Science ﹠ Technology Center ecological garden of experiment centre,
For trying area: be respectively 10 mu, 100 mu,
Experimental cultivar: 43 peach varieties,
Controlling object: peach aphid.
Result of study shows: 150 times of control efficiency ideals of elscholtiza class biopesticide dilution, it is effective to kill aphid.Small size aphid lethality is 76.1%, and large tracts of land is 94.1%.Result of study is further proof also: this life agricultural chemicals is a kind ofly can improve the plant disease-resistant ability, and the getting fat effect is arranged, and has environmental protection, pollution-free, low toxicity, does not produce the pure-natural biological agricultural chemicals of poisoning to people, animal, birds safety, to peach.
Embodiment
Embodiment 1
1, preparation elscholtiza elaboration
Selected Mosla chinensis 17.5g, wild marjoram 10.5g, Elsholtzia densa 14g, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth) 7g;
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract is at decompression 680mmHg), 40 ℃ of low temperature) vacuum drying gets dry extract down, dried cream pulverizes in cracker. cross 80 mesh sieves, make the elscholtiza elaboration.
2, getting the elscholtiza elaboration 35 gram adding low-carbon alcohols solvents 105 that make restrains through ultrasonic processing after 20 minutes, adding synergist hydrochloric acid berberine 7 grams, surfactant aliphatic acid polyethenoxy ether 1.75 grams and 8575 liters of abundant stirrings of deionized water again waits to dissolve, this biopesticide is made in filtering, packing.
Embodiment 2
1, preparation elscholtiza elaboration
Selected Mosla chinensis 26.25g, wild marjoram 17.5g, Elsholtzia densa 7g, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth) 7g;
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract gets dry extract in decompression 680mmHg, 40 ℃ of following vacuum dryings of low temperature, dried cream is pulverized in cracker. and cross 80 mesh sieves, make the elscholtiza elaboration.
2, get the elscholtiza elaboration 57.75g that makes and add low-carbon alcohols solvent 140g, through ultrasonic processing after 20 minutes, add 8575 liters of abundant stirrings of synergist fumaric acid 14g, surfactant polyoxyethylene ether 5.25g and deionized water again and wait to dissolve, this biopesticide is made in filtering, packing.
Embodiment 3
1, preparation elscholtiza elaboration
Selected Mosla chinensis 175g, wild marjoram 105g, Elsholtzia densa 140g, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth) 7g;
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract is at decompression 680mmHg), 40 ℃ of low temperature) vacuum drying gets dry extract down, dried cream pulverizes in cracker. cross 80 mesh sieves, make the elscholtiza elaboration.
2, get the elscholtiza elaboration 87.5g that makes, add low-carbon alcohols solvent 140g, after 20 minutes, add 8575 liters of abundant stirrings of synergist acrylic acid 14g, surfactant sorbitan fatty acid ester 5.25g and deionized water again and wait to dissolve through ultrasonic processing, this biopesticide is made in filtering, packing.
Embodiment 4
1, preparation elscholtiza elaboration
Selected Mosla chinensis 35g, wild marjoram 17.5g.
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract is at decompression 680mmHg), 40 ℃ of low temperature) vacuum drying gets dry extract down, dried cream pulverizes in cracker. cross 80 mesh sieves, make the elscholtiza elaboration.
2, elscholtiza elaboration 52.5 grams that make being added low-carbon alcohols solvent 105 restrains through ultrasonic processing after 20 minutes, adding synergist glycol ether 7 grams, surfactant aralkyl phenolic ether 1.75 grams and abundant stirring of deionized water 8575 grams again waits to dissolve, this biopesticide is made in filtering, packing.
Embodiment 5
1, preparation elscholtiza elaboration
Selected Mosla chinensis 17.5g, wild marjoram 35g.(every part of 1.75g)
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract is at decompression 680mmHg), 40 ℃ of low temperature) vacuum drying gets dry extract down, dried cream pulverizes in cracker. cross 80 mesh sieves, make the elscholtiza elaboration., acrylic acid.
2, elscholtiza elaboration 52.5 grams that make being added low-carbon alcohols solvent 105 restrains through ultrasonic processing after 20 minutes, adding synergist glycol ether 7 grams, surfactant alkylsulfonate 5.25 grams and abundant stirring of deionized water 8575 grams again waits to dissolve, this biopesticide is made in filtering, packing.
Embodiment 6
1, preparation elscholtiza elaboration
Selected Mosla chinensis 17.5g, wild marjoram 10.5g, Elsholtzia densa 14g;
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract is at decompression 680mmHg), 40 ℃ of low temperature) vacuum drying gets dry extract down, dried cream pulverizes in cracker. cross 80 mesh sieves, make the elscholtiza elaboration.
2, getting the elscholtiza elaboration 35 gram adding low-carbon alcohols solvents 105 that make restrains through ultrasonic processing after 20 minutes, adding synergist hydrochloric acid berberine 7 grams, surfactant polyoxyethylene carboxylate 1.75 grams and 8575 liters of abundant stirrings of deionized water again waits to dissolve, this biopesticide is made in filtering, packing.
Embodiment 7
1, preparation elscholtiza elaboration
Wild marjoram 17.5g, Elsholtzia densa 14g, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth) 7g;
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract is at decompression 680mmHg), 40 ℃ of low temperature) vacuum drying gets dry extract down, dried cream pulverizes in cracker. cross 80 mesh sieves, make the elscholtiza elaboration.
2, getting the elscholtiza elaboration 38 gram adding low-carbon alcohols solvents 105 that make restrains through ultrasonic processing after 20 minutes, adding synergist acrylic acid 7 grams, surfactant alkylnaphthalene sulfonate 1.75 grams and 8575 liters of abundant stirrings of deionized water again waits to dissolve, this biopesticide is made in filtering, packing.
Embodiment 8
1, preparation elscholtiza elaboration
Selected Mosla chinensis 26.25g, Elsholtzia densa 7g, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth) 7g;
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols with three plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract gets dry extract in decompression 700mmHg, 50 ℃ of following vacuum dryings of low temperature, dried cream is pulverized in cracker. and cross 120 mesh sieves, make the elscholtiza elaboration.
2, getting the elscholtiza elaboration 35 gram adding low-carbon alcohols solvents 105 that make restrains through ultrasonic processing after 20 minutes, adding synergist hydrochloric acid berberine 7 grams, surfactant alkylbenzenesulfonate 1.75 grams and 8575 liters of abundant stirrings of deionized water again waits to dissolve, this biopesticide is made in filtering, packing.
Embodiment 9
1, preparation elscholtiza elaboration
Selected Mosla chinensis 21g, wild marjoram 14g, Elsholtzia eriostachya Benth. (Aphanochilus eriostachya Benth, Elsholtzia pusilla Banth) 7g;
With the above-mentioned raw materials segment, clean, after the drying and crushing, with concentration be 40-95% the organic solvent low-carbon alcohols by four plant species medicines by weight composition: low-carbon alcohols=1:6 the proportioning extraction, concentrate, getting medicinal extract proportion is 1.0, medicinal extract gets dry extract in decompression 700mmHg, 50 ℃ of following vacuum dryings of low temperature, dried cream is pulverized in cracker. and cross 80 mesh sieves, make the elscholtiza elaboration.
2, get elscholtiza elaboration 42 grams that make and add low-carbon alcohols solvent 140 grams, through ultrasonic processing after 20 minutes, add synergist glycol ether 14 grams, surfactant alkyl amino-oxide 5.25 grams and 8575 liters of abundant stirrings of deionized water again and wait to dissolve, this biopesticide is made in filtering, packing.