CN106478275A - A kind of composite pesticide of preventing and treating tobacco diseases and preparation method thereof - Google Patents
A kind of composite pesticide of preventing and treating tobacco diseases and preparation method thereof Download PDFInfo
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- CN106478275A CN106478275A CN201610926221.0A CN201610926221A CN106478275A CN 106478275 A CN106478275 A CN 106478275A CN 201610926221 A CN201610926221 A CN 201610926221A CN 106478275 A CN106478275 A CN 106478275A
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- C—CHEMISTRY; METALLURGY
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- C05C11/00—Other nitrogenous fertilisers
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Abstract
The invention provides a kind of composite pesticide of preventing and treating tobacco diseases, belongs to technical field of pesticide.A kind of composite pesticide of preventing and treating tobacco diseases, it is prepared from by fowl and animal excrement, sawdust, attapulgite powder, nitrogen-phosphorus-potassium compound fertilizer, compound amino acid, ferrous sulfate, licorice section, Chinese herbaceous peony, clover, felwort and insecticide, the insecticide is made up of the raw material of following weight parts：0.5 ~ 3 part of pyrolkigneous liquid, 2 ~ 6 parts of chitin, 0.5 ~ 5 part of matrine, 5 ~ 15 parts of buckeye, 5 ~ 15 parts of argy wormwood, 3 ~ 7 parts of lychee exocarp, 3 ~ 7 parts of lemon peel, 3 ~ 7 parts of luffa pericarp, 3 ~ 7 parts of yellow cinnamon leaf, 5 ~ 10 parts of rosemary, 5 ~ 10 parts of the capsule of weeping forsythia, 5 ~ 10 parts of calamus.The each raw material Synergistic of the present invention, gained composite pesticide fertility are strong, and antibacterial ability is notable, and tobacco P substantially reduces, not only efficient but also saving of work and time.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of composite pesticide of preventing and treating tobacco diseases and its preparation side
Method.
Background technology
Tobacco drafts a document eggplant wood, the annual or limited herbaceos perennial of Solanaceae, base portion slightly lignifying.Inflorescence basidixed, circle
Taper, spends more；Capsule ovate or square round shape, length are approximately equal to persistent calyx.Summer and autumn yields positive results.It is distributed mainly on South America, south
Sub-, Chinese.Tobacco decapacitation is made cigarette, tobacco, pipe tobacco, cigar etc. and is sucked outward for people, still has multiple medical applications.Although cigarette
Grass gives people a lot of harm of class band, or even is referred to as " poisonous weeds ", the restricted circulation of many country plaintexts or suction, and the world defends
Raw organizational member also endorsed《The Framework Convention on Tobacco Control》.But as a kind of time-honored medicinal plant, its medical value
Can not be obliterated because of its harmfulness.《National Chinese herbal medicine compilation》Record, tobacco is warm in nature sweet, poisonous, with detumescence, removing toxic substances,
The effects such as desinsection, it is mainly used in furuncle swelling toxin, favus of the scalp, tinea alba, the favus of the scalp, the disease such as venomous snake bite, can also treat a subcutaneous ulcer, carbuncle on the back, wind
Phlegm, crane knee（Including bone tuberculosis, chronic purulent gonitis etc.）Deng disease.
Tobacco is easily endangered by multiple diseases during planting, and the species survey of disease is many, distribution is wide, it is fast to propagate and
Damage losses are serious.China's tobacco diseases there are about more than 60 kinds, wherein occur universal or heavier have kind more than 10.These diseases are to cigarette
Grass production causes very big economic loss.Therefore, preventing and treating tobacco diseases are the important measures for ensureing tobacco good quality and high output.
Be presently used for tobacco chemical insecticide topmost have three kinds, including pyrethroid, organophosphorus insecticide and
Carbamate pesticide, the application of these chemical insecticides, it is ensured that the significantly volume increase of agricultural livestock products, but also give people class
It is unfavorable that the health of the environment of existence, the ecological balance and mankind itself is brought, such as soil, water body, the pollution of air and agricultural livestock products
The residual hazard of product, causes substantial amounts of person poultry poisoning and death every year, and chemical insecticide has also injured insect while insect is killed
Natural enemy, destroy the ecological balance, so that some insects is increased sharply and ferocious stick up, it is difficult to preventing and treating and form vicious circle, Reusability chemistry
Insecticide, makes insect develop immunity to drugs rapidly, so that drug effect is declined or fail.While the update of chemical insecticide, develops generation
Valency more and more higher, very risky.The going deep into of strict demand especially to environmental protection and toxicity research, to new pest control with insecticide
The restriction of agent and require more and more tighter.Under these circumstances, force people to look for the new way of insect prevention, the research of insecticide and
Exploitation is not likely to produce the resistance to the action of a drug along efficient, low toxicity, insect, and the nuisanceless direction of protection pest natural enemy is developed.At present, studied
The source of manure is combined with disease prevention preparation drug fertilizer is prepared, while increasing production and improying productivity, antibacterial killing ability is improved, while reducing agriculture
The use of medicine and its impact to ecology and destruction.
The patent of composite pesticide Application No. 201010198773 discloses a kind of special base for ecological tobacco leaf planting
Fertilizer, is made up of following components material and percetage by weight：Fermented rape cake 19-23%；Biogas residue 43-47%；Normal superphosphate 17-
21%；Potassium nitrate 2.3-2.7%；Borax 0.27-0.32%；White vitriol 0.8-0.9%；Potassium sulfate 9.5-9.9%；Potassium humate
0.05-0.15%；Mixing bacterial manure 0.64-0.68%；It follow these steps to prepare：(1) cake fertilizer and biogas residue are dried, are crushed, and are substantially mixed
Compound；(2) mixing bacterial manure is added；(3) general calcium, potassium nitrate, borax, white vitriol, potassium sulfate and potassium humate are added.
The invention can be repaired or soil protection micro-ecological environment, strengthen cigarette strain resistance against diseases, lift Flue-cured tobacco Quality, give birth to ecological tobacco leaf
Produce Sustainable Healthy Development.The patent of Application No. 201610169191 discloses a kind of efficient tobacco biological medicine fertilizer, its proportioning
Scheme is as follows：10% ~ 30% bacillus licheniformis；20% ~ 40% Xanthohumate；10% ~ 30% bacillus subtilis；10%~20%DA-6.
The invention biological medicine fertilizer improve tobacco plants to fertilizer while absorbing, accomplish safe pesticide-germicide, meet
Tobacco agriculture production and the needs of development.Above-mentioned technology employs the biological source medical fertilizer of low toxicity, environmental protection to some extent, will kill
Worm agent is combined with the source of manure, raising tobacco leaf resistance against diseases disease while augment nutritional, but while what the source of manure was combined with agricultural chemicals
Stability, and antibacterial effect etc. need to improve further.
Content of the invention
In view of this, the technical problem to be solved is to provide a kind of composite pesticide of preventing and treating tobacco diseases, property
Matter is stable, antibacterial good disinsection effect, strong drug action and environmentally safe.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：20 ~ 50 parts of fowl and animal excrement, saw
End 5 ~ 10 parts, 5 ~ 10 parts of attapulgite powder, 10 ~ 20 parts of nitrogen-phosphorus-potassium compound fertilizer, 5 ~ 10 parts of compound amino acid, 1 ~ 5 part of ferrous sulfate,
5 ~ 10 parts of licorice section, 5 ~ 10 parts of Chinese herbaceous peony, 5 ~ 10 parts of clover, 5 ~ 10 parts of felwort, 1 ~ 5 part of insecticide, the insecticide by under
The raw material for stating weight portion is made：0.5 ~ 3 part of pyrolkigneous liquid, 2 ~ 6 parts of chitin, 0.5 ~ 5 part of matrine, 5 ~ 15 parts of buckeye, argy wormwood 5
~ 15 parts, 3 ~ 7 parts of lychee exocarp, 3 ~ 7 parts of lemon peel, 3 ~ 7 parts of luffa pericarp, 3 ~ 7 parts of yellow cinnamon leaf, 5 ~ 10 parts of rosemary, the capsule of weeping forsythia 5 ~ 10
Part, 5 ~ 10 parts of calamus.
Preferably, the compound amino acid be the serine of 5 ~ 10 weight portions, the methionine of 5 ~ 10 weight portions, 1 ~ 5 weight
The mixture of the glycine of the isoleucine and 0.5 ~ 2 weight portion of amount part.
Preferably, the insecticide is made up of the raw material of following weight parts：1 ~ 2 part of pyrolkigneous liquid, 3 ~ 4 parts of chitin, kuh-seng
1 ~ 3 part of alkali, 8 ~ 12 parts of buckeye, 8 ~ 12 parts of argy wormwood, 4 ~ 6 parts of lychee exocarp, 4 ~ 6 parts of lemon peel, 4 ~ 6 parts of luffa pericarp, yellow cinnamon leaf 4 ~ 6
Part, 6 ~ 7 parts of rosemary, 6 ~ 7 parts of the capsule of weeping forsythia, 8 ~ 9 parts of calamus.
Preferably, the insecticide is obtained by following preparation methods, is comprised the following steps：
1）Buckeye, argy wormwood, lychee exocarp, luffa pericarp, rosemary, the capsule of weeping forsythia and the Chang of dry above-mentioned weight are weighed respectively
Pu, mixing, crushed after freezing under nitrogen protection, obtained comminuting matter I；Dry above-mentioned weight is weighed respectively
Lemon peel and yellow cinnamon leaf, mixing, crush, obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, is heated to 40 ~ 50 DEG C, kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；The water of 2 ~ 3 times of its weight is added in the dregs of a decoction I, is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 medicinal extract, adds volume fraction in the medicinal extract
For 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, kept for 18 ~ 24 hours, then filter, filtrate III and sediment is obtained, by institute
State the lotion that relative density when filtrate III is evaporated to 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with dichloromethane, sub-cloud solution, vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, is mixed with the pyrolkigneous liquid, chitin and matrine, obtain final product and kill
Worm agent.
Preferably, the w/v 1g of the medicinal extract and the ethanol：10~20ml.
A kind of method of the composite pesticide for preparing preventing and treating tobacco diseases, comprises the following steps：
1）Weigh 5 ~ 10 parts of licorice section, 5 ~ 10 parts of Chinese herbaceous peony, 5 ~ 10 parts of clover and 5 ~ 10 parts of the felwort of weight portion respectively, mix
Close, abundant crushed after being dried；Then sequentially add 20 ~ 50 parts of the fowl and animal excrement of weight portion, 5 ~ 10 parts of sawdust, attapulgite powder 5 ~
10 parts, 10 ~ 20 parts of nitrogen-phosphorus-potassium compound fertilizer, 5 ~ 10 parts of compound amino acid and 1 ~ 5 part of ferrous sulfate, after mixing, add water wetting, adopts
It is 6 ~ 8 that citric acid or lime stone adjust pH, and control humidity is 35 ~ 40%, and temperature is 55 ~ 65 DEG C, is covered using plastic foil, banks up
7 ~ 10 days, obtain windrow；
2）1 ~ 5 weight portion of insecticide is weighed, with the abundant mix of the windrow, obtains final product composite pesticide.
Compared with prior art, its advantage is as follows for the present invention：
1）The invention provides a kind of tobacco leaf special bio compounded pesticide, enables to transplanting tobacco seedlings and quickly adapts to plantation
Soil, fast-growth, while strengthening the resistance against diseases of tobacco seedlings and soil, reduces disease hidden danger.Composite pesticide of the present invention is by multiple
Material is combined by scientific matching, not only nutritious, comprehensive, is adapted to the growth demand of tobacco seedlings, and is increased concave convex rod
Native powder enables nutrient content slowly to be discharged, maintain the longer time nutrition follow-up, while add licorice section, Chinese herbaceous peony,
Clover and felwort, are not only supplemented the nutrient content of soil, and are coordinated with insecticide, additionally it is possible to further enhance compound
The resistance against diseases of agricultural chemicals, tobacco seedlings root after active ingredient interior suction, will can strengthen the ability that tobacco seedlings resist disease, significantly reduce
The ill probability of tobacco seedlings.
2）Composite pesticide of the present invention can be used to prevent and treat the multiple diseases that tobacco seedlings occur in whole growth cycle, reduce ill several
Rate, improves tobacco seedlings survival rate and the speed of growth, and the disease that can be prevented and treated mainly includes tobacco mosaic disease, wildfire, tobacco
Anthracnose and frog-eye leaf spot of tobacco, field test find tobacco mosaic disease the incidence of disease have dropped 52.1%, disease-resistant rate be 92.7 ~
99.5%；The incidence of disease of wildfire have dropped 71.6%, and disease-resistant rate is 93.5 ~ 99.2%；The incidence of disease of anthracnose of tobacco declines
76.2%, disease-resistant rate is 96.4 ~ 100%；The incidence of disease of frog-eye leaf spot of tobacco have dropped 61.8%, and disease-resistant rate is 95.1 ~ 100%.Cause
This, applies composite pesticide of the present invention significantly improve the disease-resisting, insecticidal ability of tobacco, and simultaneous effect of controlling is projected, efficacy stability, tool
Have a wide range of applications.
Specific embodiment
For a better understanding of the present invention, with reference to embodiment present disclosure fairly set out further, but this
Bright protection content is not limited solely to the following examples.In the following description, give a large amount of concrete details so as to
More thorough understanding of the invention is provided.It will be apparent, however, to one skilled in the art that the present invention is permissible
It is carried out without the need for one or more of these details.
Embodiment 1：A kind of composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Manure of livestock and poultry
Just 40 parts, 8 parts of sawdust, 8 parts of attapulgite powder, 15 parts of nitrogen-phosphorus-potassium compound fertilizer, 8 parts of compound amino acid, 3 parts of ferrous sulfate, Radix Glycyrrhizae
8 parts of section, 8 parts of Chinese herbaceous peony, 8 parts of clover, 8 parts of felwort, 3 parts of insecticide, the insecticide is made up of the raw material of following weight parts：
1.5 parts of pyrolkigneous liquid, 3.5 parts of chitin, 2 parts of matrine, 10 parts of buckeye, 10 parts of argy wormwood, 5 parts of lychee exocarp, lemon peel 5 part, thread
5 parts of melon skin, 5 parts of yellow cinnamon leaf, 6 parts of rosemary, 7 parts of the capsule of weeping forsythia, 9 parts of calamus.
In the present invention, in nitrogen-phosphorus-potassium compound fertilizer, the mass ratio of N P and K is preferably 1.5 ~ 2:3~4:2 ~ 3, this ratio and entirety
Farm chemical ingredients are adapted, while disclosure satisfy that the growth needs of tobacco.Fowl and animal excrement used by the present invention preferably adopts pig manure, chicken manure
With the mixture of cow dung, wherein pig manure：Chicken manure：The part by weight of cow dung is 1:2:1, under here cooperation and ratio, after preparation
Property is more stable, more preferable with the compounding effect of raw material.
Compound amino acid described in the present embodiment is the serine of 6 weight portions, the methionine of 8 weight portions, 3 weight portions different
The mixture of the glycine of leucine and 0.8 weight portion.
The present invention is raw materials used to be low toxicity, environmental protection raw material.Research shows that pyrolkigneous liquid has bacteriostatic activity, preferably corn
Core pyrolkigneous liquid；Chitin is a kind of macromolecule polysaccharide of line style, and with antibacterial insecticidal activity, preferred molecular weight is 1500 ~ 3000
Chitin, compound after antibacterial killing ability higher；Matrine be by the dry root of legume kuh-seng, plant, fruit warp
The Solvent Extract methods such as ethanol are made, and are alkaloids, are the green plants source pesticides for using at present, are wide spectrum composite pesticide,
Have and tag and stomach poison function；Insecticide is formed by the mixing of plurality of raw materials medicine is compounding, nontoxic, can not only antibacterial desinsection, and
It is applied to tobacco and there is promotion growth, improves quality of tobacco and other effects.After above-mentioned raw materials are by rationally compounding, mutual help,
There is obvious prevention effect to the disease that the whole growth cycle of tobacco occurs, good absorbing effect, non-target object is not appointed
What injures.
In the present invention, buckeye is the dry mature seed of Aesculus, surface brown or sepia, many shrinkages, concavo-convex not
Put down, somewhat gloss.Argy wormwood is perennial herb or slightly becomes suffruticose plant, and plant has enhanced aroma, modern experimental research card
Bright, tarragon has antibacterial and antivirus action；Relieving asthma, antibechic and phlegm-dispelling functions；Hemostasis and blood coagulation resisting function；Calm and antiallergy
Effect；Protect liver choleretic effect etc..Lychee exocarp, is the pericarp of sapindaceous plant lichee.Nature and flavor are bitter, cold.There is dehumidifying and stop dysentery, only
Blood is acted on.Now there are some researches show, containing tens kinds of compositions such as Flavonoid substances in litchi rind.Lemon peel, is rutaceae multitude
Lemon or the pericarp of lemon, gas are fragrant, mildly bitter flavor, and extract contains multiple bacteriostatic active ingredients.Luffa pericarp, is the pericarp of sponge gourd, food
With can be clearing heat and detoxicating.Yellow cinnamon leaf, is the fresh leaf of yellow camphor tree, mainly contains volatile oil component, wherein 1, 8-Cineole content about 60%, camphor tree
Brain about 0.75%, other compositions have citral, capraldehyde, australene, amphene, α-terpinene, cymol, linalool, α-pine
Oleyl alcohol and benzyl system sequiterpene etc..Rosemary, is the herb of labiate rosemary, and contained volatile oil is to Staphylococcus aureus
Bacterium, Escherichia coli, comma bacillus etc. have certain antibacterial action.The capsule of weeping forsythia, also known as chrysanthemum bar, even shell, green grass or young crops stick up, fall to sticking up, Huang Qidan
Deng, containing Neolignans, flavone compound, styrenic derivatives etc., the pharmacology such as have antibacterial, cardiac stimulant, diuresis, town to tell
Effect.Calamus, can for ward off dirty have one's ideas straightened out, a surname's gas detoxifies by phlegm, desinsection.
Method for producing insecticide described in the present embodiment, comprises the following steps：
1）Dry above-mentioned buckeye, argy wormwood, lychee exocarp, luffa pericarp, rosemary, the capsule of weeping forsythia and calamus are weighed respectively, mixing, freezing
Crushed under nitrogen protection afterwards, obtained comminuting matter I；Dry above-mentioned lemon peel and yellow cinnamon leaf are weighed respectively, mixing, powder
Broken, obtain comminuting matter II；
2）The water of 5 times of its weight is added in the comminuting matter I, is heated to 50 DEG C, kept for 45 minutes, filter while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；The water of 2 times of its weight is added in the dregs of a decoction I, is heated to 55 DEG C, keep
35 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged filtrate is obtained, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.13 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
28 DEG C, kept for 24 hours, then filter, filtrate III and sediment is obtained, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.23 lotion；
3）The water of its 4 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 38 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, is mixed with the pyrolkigneous liquid, chitin and matrine, obtain final product and kill
Worm agent.
The medicinal extract is 1g with the w/v of the ethanol：16ml.
The method that the present embodiment prepares the composite pesticide of preventing and treating tobacco diseases, comprises the following steps：
1）Licorice section, Chinese herbaceous peony, clover and the felwort of above-mentioned weight portion are weighed respectively, mixing, abundant crushed after being dried；Then
Fowl and animal excrement, sawdust, attapulgite powder, nitrogen-phosphorus-potassium compound fertilizer, compound amino acid and the sulfuric acid for sequentially adding above-mentioned weight portion is sub-
Iron, after mixing, add water wetting, and it is 6 ~ 8 to adjust pH using citric acid or lime stone, and control humidity is 38%, and temperature is 60 DEG C, adopts
Covered with plastic foil, bank up 8 ~ 9 days, obtain windrow；
2）3 weight portion of insecticide is weighed, with the abundant mix of the windrow, obtains final product composite pesticide.
By the combination of preparation method, the property of the composite pesticide of present invention preventing and treating tobacco diseases is more stable, and environment is fitted
Answering property is more preferable.
The composite pesticide of gained preventing and treating tobacco diseases of the present invention can be used as base manure.
Embodiment 2：A kind of composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Manure of livestock and poultry
Just 30 parts, 6 parts of sawdust, 6 parts of attapulgite powder, 12 parts of nitrogen-phosphorus-potassium compound fertilizer, 6 parts of compound amino acid, 4 parts of ferrous sulfate, Radix Glycyrrhizae
6 parts of section, 7 parts of Chinese herbaceous peony, 6 parts of clover, 9 parts of felwort, 2 parts of insecticide, the insecticide is made up of the raw material of following weight parts：
1 part of pyrolkigneous liquid, 4 parts of chitin, 1 part of matrine, 8 parts of buckeye, 8 parts of argy wormwood, 4 parts of lychee exocarp, 4 parts of lemon peel, luffa pericarp 4
Part, 4 parts of yellow cinnamon leaf, 7 parts of rosemary, 6 parts of the capsule of weeping forsythia, 8 parts of calamus.
The compound amino acid is the serine of 7 weight portions, the methionine of 7 weight portions, the isoleucine of 3.5 weight portions
Mixture with the glycine of 1 weight portion.
Described in the present embodiment, method for producing insecticide is comprised the following steps：
1）Buckeye, argy wormwood, lychee exocarp, luffa pericarp, rosemary, the capsule of weeping forsythia and the Chang of dry above-mentioned weight are weighed respectively
Pu, mixing, crushed after freezing under nitrogen protection, obtained comminuting matter I；Dry above-mentioned weight is weighed respectively
Lemon peel and yellow cinnamon leaf, mixing, crush, obtain comminuting matter II；
2）The water of 4 times of its weight is added in the comminuting matter I, is heated to 40 DEG C, kept for 45 minutes, filter while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；The water of 2 times of its weight is added in the dregs of a decoction I, is heated to 50 DEG C, keep
38 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged filtrate is obtained, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.15 medicinal extract, adds the ethanol solution that volume fraction is 80%, temperature in the medicinal extract
25 DEG C, kept for 24 hours, then filter, filtrate III and sediment is obtained, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.25 lotion；
3）The water of its 3 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 35 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, is mixed with the pyrolkigneous liquid, chitin and matrine, obtain final product and kill
Worm agent.
The medicinal extract is 1g with the w/v of the ethanol：12ml.
The method that the present embodiment prepares the composite pesticide of preventing and treating tobacco diseases, comprises the following steps：
1）Licorice section, Chinese herbaceous peony, clover and the felwort of above-mentioned weight portion are weighed respectively, mixing, abundant crushed after being dried；Then
Fowl and animal excrement, sawdust, attapulgite powder, nitrogen-phosphorus-potassium compound fertilizer, compound amino acid and the sulfuric acid for sequentially adding above-mentioned weight portion is sub-
Iron, after mixing, add water wetting, and it is 6 ~ 8 to adjust pH using citric acid or lime stone, and control humidity is 36%, and temperature is 56 DEG C, adopts
Covered with plastic foil, bank up 7 ~ 8 days, obtain windrow；
2）2 weight portion of insecticide is weighed, with the abundant mix of the windrow, obtains final product composite pesticide.
Embodiment 3：A kind of composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Manure of livestock and poultry
Just 35 parts, 9 parts of sawdust, 9 parts of attapulgite powder, 18 parts of nitrogen-phosphorus-potassium compound fertilizer, 9 parts of compound amino acid, 4 parts of ferrous sulfate, Radix Glycyrrhizae
9 parts of section, 6 parts of Chinese herbaceous peony, 9 parts of clover, 9 parts of felwort, 4 parts of insecticide, the insecticide is made up of the raw material of following weight parts：
2 parts of pyrolkigneous liquid, 3 parts of chitin, 3 parts of matrine, 12 parts of buckeye, 12 parts of argy wormwood, 6 parts of lychee exocarp, 6 parts of lemon peel, luffa pericarp 6
Part, 6 parts of yellow cinnamon leaf, 7 parts of rosemary, 7 parts of the capsule of weeping forsythia, 8 parts of calamus.
The compound amino acid is the serine of 8 weight portions, the methionine of 6 weight portions, the isoleucine of 4 weight portions and
The mixture of the glycine of 1.5 weight portions.
Described in the present embodiment, method for producing insecticide is comprised the following steps：
1）Buckeye, argy wormwood, lychee exocarp, luffa pericarp, rosemary, the capsule of weeping forsythia and the Chang of dry above-mentioned weight are weighed respectively
Pu, mixing, crushed after freezing under nitrogen protection, obtained comminuting matter I；Dry above-mentioned weight is weighed respectively
Lemon peel and yellow cinnamon leaf, mixing, crush, obtain comminuting matter II；
2）The water of 6 times of its weight is added in the comminuting matter I, is heated to 50 DEG C, kept for 45 minutes, filter while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；The water of 3 times of its weight is added in the dregs of a decoction I, is heated to 60 DEG C, keep
40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged filtrate is obtained, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.12 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
30 DEG C, kept for 20 hours, then filter, filtrate III and sediment is obtained, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.21 lotion；
3）The water of its 5 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 40 minutes, and distillation is extracted 3 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, is mixed with the pyrolkigneous liquid, chitin and matrine, obtain final product and kill
Worm agent.
The medicinal extract is 1g with the w/v of the ethanol：18ml.
The method that the present embodiment prepares the composite pesticide of preventing and treating tobacco diseases, comprises the following steps：
1）Licorice section, Chinese herbaceous peony, clover and the felwort of above-mentioned weight portion are weighed respectively, mixing, abundant crushed after being dried；Then
Fowl and animal excrement, sawdust, attapulgite powder, nitrogen-phosphorus-potassium compound fertilizer, compound amino acid and the sulfuric acid for sequentially adding above-mentioned weight portion is sub-
Iron, after mixing, add water wetting, and it is 6 ~ 8 to adjust pH using citric acid or lime stone, and control humidity is 35%, and temperature is 65 DEG C, adopts
Covered with plastic foil, bank up 9 ~ 10 days, obtain windrow；
2）4 weight portion of insecticide is weighed, with the abundant mix of the windrow, obtains final product composite pesticide.
Embodiment 4：A kind of composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Manure of livestock and poultry
Just 20 parts, 5 parts of sawdust, 5 parts of attapulgite powder, 10 parts of nitrogen-phosphorus-potassium compound fertilizer, 5 parts of compound amino acid, 1 part of ferrous sulfate, Radix Glycyrrhizae
5 parts of section, 5 parts of Chinese herbaceous peony, 5 parts of clover, 5 parts of felwort, 1 part of insecticide, the insecticide is made up of the raw material of following weight parts：
0.5 part of pyrolkigneous liquid, 6 parts of chitin, 0.5 part of matrine, 5 parts of buckeye, 5 parts of argy wormwood, 3 parts of lychee exocarp, 3 parts of lemon peel, sponge gourd
3 parts of skin, 3 parts of yellow cinnamon leaf, 5 parts of rosemary, 5 parts of the capsule of weeping forsythia, 5 parts of calamus.
The preparation method of composite pesticide in the present embodiment, method for producing insecticide refer to embodiment 1, repeat no more.
Embodiment 5：A kind of composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Manure of livestock and poultry
Just 50 parts, 10 parts of sawdust, 10 parts of attapulgite powder, 20 parts of nitrogen-phosphorus-potassium compound fertilizer, 10 parts of compound amino acid, 5 parts of ferrous sulfate, sweet
10 parts of grass section, 10 parts of Chinese herbaceous peony, 10 parts of clover, 10 parts of felwort, 5 parts of insecticide, the insecticide is by the original of following weight parts
Material is made：3 parts of pyrolkigneous liquid, 2 parts of chitin, 5 parts of matrine, 15 parts of buckeye, 15 parts of argy wormwood, 7 parts of lychee exocarp, 7 parts of lemon peel,
7 parts of luffa pericarp, 7 parts of yellow cinnamon leaf, 10 parts of rosemary, 10 parts of the capsule of weeping forsythia, 10 parts of calamus.
The compound amino acid is the serine of 5 weight portions, the methionine of 10 weight portions, the isoleucine of 5 weight portions
Mixture with the glycine of 0.5 weight portion.
The preparation method of composite pesticide in the present embodiment, method for producing insecticide refer to embodiment 1, repeat no more.
Embodiment 6：A kind of composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Manure of livestock and poultry
Just 45 parts, 9 parts of sawdust, 8 parts of attapulgite powder, 16 parts of nitrogen-phosphorus-potassium compound fertilizer, 8 parts of compound amino acid, 4 parts of ferrous sulfate, Radix Glycyrrhizae
7 parts of section, 6 parts of Chinese herbaceous peony, 8 parts of clover, 8 parts of felwort, 3 parts of insecticide, the insecticide is made up of the raw material of following weight parts：
0.8 part of pyrolkigneous liquid, 5 parts of chitin, 4 parts of matrine, 12 parts of buckeye, 12 parts of argy wormwood, 5.5 parts of lychee exocarp, 4.5 parts of lemon peel,
6 parts of luffa pericarp, 5 parts of yellow cinnamon leaf, 9 parts of rosemary, 7 parts of the capsule of weeping forsythia, 8 parts of calamus.
The compound amino acid is the serine of 10 weight portions, the methionine of 5 weight portions, the isoleucine of 1 weight portion
Mixture with the glycine of 2 weight portions.
The preparation method of composite pesticide in the present embodiment, method for producing insecticide refer to embodiment 1, repeat no more.
Embodiment 7：The composite pesticide of the preventing and treating tobacco diseases described by the present embodiment, as different from Example 1：
The insecticide also includes：1.5 weight portion of microbial bacterial agent, the microbial bacterial agent is by 55% withered grass gemma of mass fraction
Bacillus and 45% streptomyces microflavus of mass fraction composition.
Bacillus subtilis, Bacillus subtilis, living bacteria count are 500 ~ 80,000,000,000 cfu/g；Streptomyces microflavus,
Streptomyces microflavus, living bacteria count are 2 ~ 300,000,000/g.
The present embodiment method for producing insecticide is as different from Example 1：Step 4）The lotion and extract are divided
Not dry, pulverize, mix with the pyrolkigneous liquid, chitin and matrine, the microbial bacterial agent is added, is sufficiently mixed, i.e.,
Obtain insecticide.
Embodiment 8：Described by the present embodiment preventing and treating tobacco diseases composite pesticide, with embodiment 1 except that：
The insecticide also includes：1 weight portion of microbial bacterial agent, the microbial bacterial agent is by 60% bacillus subtilis of mass fraction
Bacterium and 40% streptomyces microflavus of mass fraction composition.
Comparative example 1：The composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Fowl and animal excrement 40
Part, 8 parts of sawdust, 8 parts of attapulgite powder, 15 parts of nitrogen-phosphorus-potassium compound fertilizer, 8 parts of compound amino acid, 3 parts of ferrous sulfate, licorice section 8
Part, 8 parts of Chinese herbaceous peony, 8 parts of clover, 8 parts of felwort.
Comparative example 2：The composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Fowl and animal excrement 40
Part, 8 parts of sawdust, 8 parts of attapulgite powder, 15 parts of nitrogen-phosphorus-potassium compound fertilizer, 8 parts of compound amino acid, 3 parts of ferrous sulfate, licorice section 8
Part, 8 parts of Chinese herbaceous peony, 8 parts of clover, 8 parts of felwort, 3 parts of insecticide, the insecticide is made up of the raw material of following weight parts：Wood
1.5 parts of vinegar liquid, 3.5 parts of chitin, 2 parts of matrine.
Comparative example 3：The composite pesticide of preventing and treating tobacco diseases, is prepared from by the raw material of following weight portion：Fowl and animal excrement 40
Part, 8 parts of sawdust, 8 parts of attapulgite powder, 15 parts of nitrogen-phosphorus-potassium compound fertilizer, 8 parts of compound amino acid, 3 parts of ferrous sulfate, licorice section 8
Part, 8 parts of Chinese herbaceous peony, 8 parts of clover, 8 parts of felwort, 3 parts of insecticide, the insecticide is made up of the raw material of following weight parts：Suo
10 parts of Luo Zi, 10 parts of argy wormwood, 5 parts of lychee exocarp, 5 parts of lemon peel, 5 parts of luffa pericarp, 5 parts of yellow cinnamon leaf, 6 parts of rosemary, 7 parts of the capsule of weeping forsythia,
9 parts of calamus, remaining raw material and weight portion are with embodiment 1.
, composite pesticide effect assessment
Experimental group：A1：Embodiment 1；A2：Embodiment 7.
Control group：B1：Comparative example 1；B2：Comparative example 2；B3：Comparative example 3.
Blank group：B0：Agricultural chemicals is not used, only apply N P and K inorganic fertilizer.
Test method：The base manure that experimental group and control group gained composite pesticide are planted as tobacco seedlings is used, per mu of administration
20 ~ 25kg, does not use other medicine fertilizer.
To experimental group A1, A2 of the present invention, the disease species of control group B1, B2, B3 and the planted tobacco of blank group B0, disease
Probability of happening, tobacco transplant survival rate, yield of tobacco and first-class cigarette ratio are counted, and objects of statistics was planted for the same period
100m2Tobacco, statistics are as shown in table 1.
1 composite pesticide of table is counted to the effect of tobacco planting
Can be seen by table 1, tobacco be planted as base manure using composite pesticide of the present invention, substantially can reduce disease incidence,
Especially tobacco mosaic disease, wildfire, anthracnose of tobacco and frog-eye leaf spot of tobacco；The transplanting survival rate of experimental group tobacco is more right
Significantly improve according to group；Experimental group tobacco production is apparently higher than control group.Meanwhile, for experimental group, add microbial bacterial agent
Composite pesticide resistance against diseases increases, and transplanting survival rate is also improved therewith；For control group, without the control of insecticide
Group B1, the resistance against diseases of tobacco are substantially weaker, and P is significantly higher；And use different insecticides（Control group B2 and
B3）, the resistance against diseases of tobacco also has significantly difference, while resistance against diseases is significantly smaller than the experimental group of the present invention.And, statistics
As a result show, tobacco is planted as base manure using the composite pesticide of the present invention, the quality of tobacco leaf can also be improved, on increasing
Ratio Deng cigarette.
Additionally, the source of manure and insecticide are combined as base manure plantation tobacco by the present invention can dramatically increase yield of tobacco, real
Group A1 is tested compared with B0, improve 2.0 times of yield of tobacco；Experimental group A2 improves 2.3 times of yield of tobacco compared with B0；Control group B1
Compared with B0, yield of tobacco 40.0% is improved；Control group B2 improves yield of tobacco 70.0% compared with B0；Control group B2 and B0 phase
Than improving 1.0 times of yield of tobacco.Wherein, the yield increase rate highest of experimental group A2, A1 are taken second place, obviously higher than control group B1 ~
B3.
Above-mentioned as shown by data, the fertilizer efficiency of composite pesticide of the present invention are notable, can significantly improve the yield of tobacco leaf；Meanwhile, this
Invention composite pesticide can significantly reduce the probability of tobacco illness insect pest, and then improve the yield of tobacco leaf.
, insecticide effect assessment
Antibacterial tests
1.1 reagent agent
Experimental group：ⅠA：Insecticide prepared by the embodiment of the present invention 1；
ⅠB：Insecticide prepared by the embodiment of the present invention 7；
Control group：ⅡA：Insecticide prepared by comparative example 2；
ⅡB：Insecticide prepared by comparative example 3.
1.2 inhibitory activity are tested
1）Assay method 1
For trying germ：Tobacco mosaic virus (TMV)（Tobacco mosaic virus）.
Test plant：Withered spot host's Nicotiana glutinosa.By Nicotiana glutinosa seed first with the 30min that hots water treatment of seeds during nursery.Then will plant
Son is directly sowed in seedling-growing container, puts it into illumination box, 22 DEG C of temperature, illumination 16h/d, and humidity 60% is cultivated, when
Tobacco supplies examination when growing to 5 ~ 6 true leaves.
Inoculation：It is inoculated with using juice rubbing manipulation；
From 5 ~ 6 leaf phase Nicotiana glutinosas as the withered spot host for supplying examination germ；
Test is carried out in insect prevention greenhouse.Reagent agent is taken, the 0.05g/ml aqueous solution is configured to distilled water, add for examination disease
Bacterium, makes the final concentration of 10 μ g/ml of virus.Mixing 10min, left using half leaf method frictional inoculation in withered spot host Nicotiana glutinosa（Right）Half
Ye Shang, separately with the distilled water containing TMV virus（The final concentration of 10 μ g/ml of virus）For control, 5 leaves of inoculation, test weight is often processed
Multiple 3 times.After 3D after obvious withered spot to appear, record withered spot number, calculates inhibiting rate in time（%）, commented with average inhibition
Valency inhibition；
Inhibiting rate=（Control withered spot number-process withered spot number）/ control withered spot number × 100.
2）Assay method 2
For trying germ：Anthrax-bacilus（Colletotrichum destructivum O'Gara）, tobacco tail spore bacterium（Cercospora
nicotianae Ell. et Ev.）, prairie fire germ（Pseudomonas tabaci (Wolf et Foster)
Stevens）.
Reagent agent adopts sessile drop method to pathogen conidia germination inhibitory action, adds the suppression of 0.1% mercuric chloride before counting
Its rudiment, the different visuals field count more than 100 spores, the little micro dmso solution of the crude extract for being not readily dissolved in water of polarity
After be diluted with water to desired concn and tested, also add same amount dimethyl sulfoxide (DMSO) in the control group aqueous solution.
The inhibitory action of pathogen mycelial growth adopts growth rate method.Under aseptic condition, with the punching that aperture is 0.8cm
Device takes the eugonic bacterial classification of colony edge and moves on the PDA culture plate containing extract 0.01g/mL that (extract is being processed
Before first pass through high-temperature sterilization, then allocate concentration with sterilized water, to reduce pollution.After the little crude extract of polarity is first dissolved in acetone
Mix with 40 ~ 50 DEG C of agar mediums again, be uniformly dispersed with profit), with culture medium, add equivalent sterilized water to process weight as control, often
Multiple 3 times, 26 DEG C incubated, when control bacterium colony is close to culture dish edge, determines colony diameter, and each bacterium colony presses right-angled intersection
Method is measured 2 times, represents the size of bacterium colony with its average, calculates inhibiting rate.
Inhibiting rate computing formula is as follows：
Inhibition of germination (%)=(control spore germination number-process spore germination number)/control spore germination number × 100；
Mycelial growth inhibition rate (%)=(the pure increment of the pure increment-process of control)/control colony diameter × 100.
1.3 reagent agents are to supplying to try the bacteriostatic activity of germ
2 reagent agent of table is to supplying to try the inhibiting rate of germ
From table 2 it can be seen that the insecticide prepared by the embodiment of the present invention is 99.2% to the inhibiting rate of tobacco mosaic virus (TMV), all
Apparently higher than comparative example 1 and 2, simultaneously above the inhibiting rate sum of comparative example 1 and 2, the purpose of Synergistic has been reached.The present invention
, to the inhibition of germination of prairie fire germ, anthrax-bacilus and tobacco tail spore bacterium all more than 85%, wherein prepared by embodiment for insecticide
Inhibition of germination highest of the insecticide to tobacco tail spore bacterium, be 96.2%, insecticide prepared by embodiment 7 is to anthrax
The inhibition of germination highest of bacterium, is 98.2%；And the spore of comparative example 1 and 2 pairs of prairie fire germs, anthrax-bacilus and tobacco tail spore bacterium
Sub- Germination suppression rate all fluctuate larger, and be less than 50%.Insecticide of the present invention is to prairie fire germ, anthrax-bacilus and tobacco tail spore bacterium
Mycelial growth inhibition rate all more than 70%, the insecticide that wherein prepared by embodiment to the mycelial growth inhibition rate of prairie fire germ most
Height, is 95.2%；Comparatively speaking, the percentage mycelial inhibition of 1 and 2 pairs of three kinds of pathogens of comparative example is substantially weaker.
Additionally, group I A and I B are Comparatively speaking, every effect of group I B is better than I A, inhibition is had and is increased
Use is pretended, can be used as more preferred scheme.Above-mentioned experiment shows that insecticide of the present invention is used for the preventing and treating of tobacco leaf germ to be had
Notable and stable inhibition, can strengthen the resistance against diseases of plant and soil as medicine fertilizer use, and strong drug action, volume increase increase
Receive, effect is significant.
Although the foregoing describing the specific embodiment of the present invention, those familiar with the art should manage
Solution, the specific embodiment described by us are merely exemplary, rather than for the restriction to the scope of the present invention, are familiar with this
Equivalent modification and change that the technical staff in field is made in the spirit according to the present invention, all should cover the present invention's
In scope of the claimed protection.
Claims (7)
1. a kind of preventing and treating tobacco diseases composite pesticide, it is characterised in that：It is prepared from by the raw material of following weight portion：Manure of livestock and poultry
Just 20 ~ 50 parts, 5 ~ 10 parts of sawdust, 5 ~ 10 parts of attapulgite powder, 10 ~ 20 parts of nitrogen-phosphorus-potassium compound fertilizer, 5 ~ 10 parts of compound amino acid, sulphur
1 ~ 5 part of acid ferrous iron, 5 ~ 10 parts of licorice section, 5 ~ 10 parts of Chinese herbaceous peony, 5 ~ 10 parts of clover, 5 ~ 10 parts of felwort, 1 ~ 5 part of insecticide, institute
State insecticide to be made up of the raw material of following weight parts：0.5 ~ 3 part of pyrolkigneous liquid, 2 ~ 6 parts of chitin, 0.5 ~ 5 part of matrine, buckeye
5 ~ 15 parts, 5 ~ 15 parts of argy wormwood, 3 ~ 7 parts of lychee exocarp, 3 ~ 7 parts of lemon peel, 3 ~ 7 parts of luffa pericarp, 3 ~ 7 parts of yellow cinnamon leaf, rosemary 5 ~ 10
Part, 5 ~ 10 parts of the capsule of weeping forsythia, 5 ~ 10 parts of calamus.
2. as claimed in claim 1 preventing and treating tobacco diseases composite pesticide, it is characterised in that：The compound amino acid is 5 ~ 10
The glycine of the serine of weight portion, the methionine of 5 ~ 10 weight portions, the isoleucine of 1 ~ 5 weight portion and 0.5 ~ 2 weight portion
Mixture.
3. as claimed in claim 2 preventing and treating tobacco diseases composite pesticide, it is characterised in that：The insecticide is by following weight
The raw material of part is made：1 ~ 2 part of pyrolkigneous liquid, 3 ~ 4 parts of chitin, 1 ~ 3 part of matrine, 8 ~ 12 parts of buckeye, 8 ~ 12 parts of argy wormwood, lichee
4 ~ 6 parts of shell, 4 ~ 6 parts of lemon peel, 4 ~ 6 parts of luffa pericarp, 4 ~ 6 parts of yellow cinnamon leaf, 6 ~ 7 parts of rosemary, 6 ~ 7 parts of the capsule of weeping forsythia, 8 ~ 9 parts of calamus.
4. as claimed in claim 3 preventing and treating tobacco diseases composite pesticide, it is characterised in that：The insecticide is by following weight
The raw material of part is made：1.5 parts of pyrolkigneous liquid, 3.5 parts of chitin, 2 parts of matrine, 10 parts of buckeye, 10 parts of argy wormwood, 5 parts of lychee exocarp,
5 parts of lemon peel, 5 parts of luffa pericarp, 5 parts of yellow cinnamon leaf, 6 parts of rosemary, 7 parts of the capsule of weeping forsythia, 9 parts of calamus.
5. as described in any one of claim 1 ~ 4 preventing and treating tobacco diseases composite pesticide, it is characterised in that：
The insecticide is obtained by following preparation methods, is comprised the following steps：
1）Buckeye, argy wormwood, lychee exocarp, luffa pericarp, rosemary, the capsule of weeping forsythia and the Chang of dry above-mentioned weight are weighed respectively
Pu, mixing, crushed after freezing under nitrogen protection, obtained comminuting matter I；Dry above-mentioned weight is weighed respectively
Lemon peel and yellow cinnamon leaf, mixing, crush, obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, is heated to 40 ~ 50 DEG C, kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；The water of 2 ~ 3 times of its weight is added in the dregs of a decoction I, is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 medicinal extract, adds volume fraction in the medicinal extract
For 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, kept for 18 ~ 24 hours, then filter, filtrate III and sediment is obtained, by institute
State the lotion that relative density when filtrate III is evaporated to 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with dichloromethane, sub-cloud solution, vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, is mixed with the pyrolkigneous liquid, chitin and matrine, obtain final product and kill
Worm agent.
6. as claimed in claim 5 preventing and treating tobacco diseases composite pesticide, it is characterised in that：The medicinal extract and the ethanol
W/v 1g：10~20ml.
7. a kind of prepare preventing and treating tobacco diseases composite pesticide method, it is characterised in that：Comprise the following steps：
1）Weigh 5 ~ 10 parts of licorice section, 5 ~ 10 parts of Chinese herbaceous peony, 5 ~ 10 parts of clover and 5 ~ 10 parts of the felwort of weight portion respectively, mix
Close, abundant crushed after being dried；Then sequentially add 20 ~ 50 parts of the fowl and animal excrement of weight portion, 5 ~ 10 parts of sawdust, attapulgite powder 5 ~
10 parts, 10 ~ 20 parts of nitrogen-phosphorus-potassium compound fertilizer, 5 ~ 10 parts of compound amino acid and 1 ~ 5 part of ferrous sulfate, after mixing, add water wetting, adopts
It is 6 ~ 8 that citric acid or lime stone adjust pH, and control humidity is 35 ~ 40%, and temperature is 55 ~ 65 DEG C, is covered using plastic foil, banks up
7 ~ 10 days, obtain windrow；
2）1 ~ 5 weight portion of insecticide is weighed, with the abundant mix of the windrow, obtains final product composite pesticide.
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| CN112479208A (en) * | 2020-12-08 | 2021-03-12 | 广东省农业科学院作物研究所 | Tobacco stem-based activated carbon electrode material self-doped with phosphorus element and preparation method and application thereof |
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| CN113854012A (en) * | 2021-09-09 | 2021-12-31 | 遵义市播州区鸭枫烤烟综合服务专业合作社 | Green prevention and control method for tobacco plant diseases and insect pests |
| CN115251051A (en) * | 2022-02-16 | 2022-11-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Application of beta-caryophyllene and beta-caryophyllene synthetase gene in preventing and treating ralstonia solanacearum and/or wildfire germ |
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| CN112466671A (en) * | 2020-12-02 | 2021-03-09 | 广东省农业科学院作物研究所 | Nitrogen-self-doped tobacco stem-based activated carbon electrode material and preparation method and application thereof |
| CN112466671B (en) * | 2020-12-02 | 2022-03-11 | 广东省农业科学院作物研究所 | Nitrogen-self-doped tobacco stem-based activated carbon electrode material and preparation method and application thereof |
| CN112479208A (en) * | 2020-12-08 | 2021-03-12 | 广东省农业科学院作物研究所 | Tobacco stem-based activated carbon electrode material self-doped with phosphorus element and preparation method and application thereof |
| CN113854012A (en) * | 2021-09-09 | 2021-12-31 | 遵义市播州区鸭枫烤烟综合服务专业合作社 | Green prevention and control method for tobacco plant diseases and insect pests |
| CN113831185A (en) * | 2021-10-27 | 2021-12-24 | 武汉爱民制药股份有限公司 | Tianshima chestnut growth regulator and application thereof |
| CN115251051A (en) * | 2022-02-16 | 2022-11-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Application of beta-caryophyllene and beta-caryophyllene synthetase gene in preventing and treating ralstonia solanacearum and/or wildfire germ |
| CN115251051B (en) * | 2022-02-16 | 2024-01-23 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Application of beta-caryophyllene and beta-caryophyllene synthetase gene in prevention and treatment of bacterial wilt and/or wild fire pathogen |
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