CN106070377A - A kind of jervine plant source compound antibacterial and application thereof - Google Patents

A kind of jervine plant source compound antibacterial and application thereof Download PDF

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CN106070377A
CN106070377A CN201610459375.3A CN201610459375A CN106070377A CN 106070377 A CN106070377 A CN 106070377A CN 201610459375 A CN201610459375 A CN 201610459375A CN 106070377 A CN106070377 A CN 106070377A
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jervine
extract
plant source
source compound
antibacterial
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CN106070377B (en
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葛喜珍
薛科科
李映
田平芳
缪刚
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Beijing Union University
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Beijing Union University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A01N25/14—Powders or granules wettable
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
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Abstract

The invention discloses a kind of jervine plant source compound antibacterial and application thereof.Described jervine plant source compound antibacterial comprises jervine 0.1% 10%, extract of willow bark 1 20% by weight percentage, and remaining is auxiliary agent；Described jervine is the sterling containing Rhizoma et radix veratri (Radix Rhizoma Veratri) total alkali, mixture or Veratrum extract.Described jervine plant source compound antibacterial is microemulsion, water preparation or wettable powder.The antibacterial of the present invention does not contains any chemical pesticide composition, is prone to degraded in the environment, and to people, animal, Environmental security, pathogen is not likely to produce Drug resistance to it；There is stable, efficient, the advantage of low cost simultaneously.The antibacterial of the present invention may be used for preventing and treating one or more in the diseases such as the brown rot of crops, gray mold, soft rot, scab, Bacterial septicaemia syndrome, downy mildew, phytophthora root rot, powdery mildew, late blight, dry rot, it is also possible to for the preventing and treating of other plant disease.

Description

A kind of jervine plant source compound antibacterial and application thereof
Technical field
The present invention relates to a kind of jervine plant source compound antibacterial and application thereof, belong to technical field of pesticide.
Background technology
Brown rot, gray mold, soft rot, scab, Bacterial septicaemia syndrome, downy mildew, phytophthora root rot, powdery mildew, late blight, Dry rots etc. are the Major Diseases on fruit tree, vegetable, and damaging range is wide, and difficulty of prevention and cure is bigger.Preventing and treating to these diseases at present Rely primarily on chemical bactericide, but long-term substantial amounts of chemical bactericide causes Drug resistance, environmental pollution, pesticide residues, seriously Harm human health.Ministry of Agriculture's " to the year two thousand twenty Pesticide use amount zero growth rate action scheme " proposes to be strict with to plant protection.Biological Pesticide (technology) is nuisanceless, green, environmental protection, becomes important channel and the study hotspot of new type bactericide initiative.
Rhizoma et radix veratri (Radix Rhizoma Veratri) (Veratrum nigrum L.) is Liliaceae Veratrum, and for herbaceos perennial, jervine is deposited Being in Liliaceae Veratrum and sabadilla platymiscium, raw material is seed and white hellebore, the rhizome of RADIX ET RHIZOMA VERATRI NIGRI of sabadilla.To plant Raw material is through ethanol extraction or extracts prepared.Insecticide, as plant insecticide, is had and tags and stomach poison function by jervine.Can For preventing and treating the sanitary insect pests such as housefly, cockroach, louse it can also be used to prevent and treat the agriculturals such as Pieris rapae, aphid, leafhopper, thrips and stinkbug Insect.Our early-stage Study finds, jervine is to plant disease such as brown rot, gray mold, soft rot, scab, bacillary Shothole disease, downy mildew, phytophthora root rot, powdery mildew, late blight, dry rot have good inhibiting effect.
Salix Salix babylonica plant amedica is with a long history, and within B.C. 1550, ancient Egypt's document is recorded, and utilizes The most grieved Compendium of Material Medica of Salix alba leaf is recorded: " willow is herbal classic low-grades, and nature and flavor bitter cold is nontoxic, can treat edema due to wind pathogen jaundice, carbuncle sore The diseases such as toxic swelling ".According to " Chinese medicine voluminous dictionary " record: " willow tender leaf or branch and leaf, its cold in nature, bitter in the mouth, nontoxic, tool dissipate wind, hold up wet, clear Damp and hot, control the effects such as icterohepatitis, rheumatic arthritis, eczema, endemic goiter ".Folks of china has willow now Leaf works as tea-drinking, to treat hypertension.Extract of willow bark, is mainly composed of salicin, hydrolyzes or aoxidizes and becomes saligenin, bigcatkin willow Acid, is clinically used for treatment heating, catches a cold and infect, and for " natural aspirin ", salicoside is used for treating slight heating, sense Emit, infect (influenza), acute and chronic rheumatism discomfort, headache and the pain caused because of inflammation.Early-stage Study finds willow Bark extract is obvious to Activities of Some Plants pathogen bacteriostasis.
Patent documentation CN103651632B (Authorization Notice No.) discloses containing Fructus Toosendan and the pesticide combination of Rhizoma et radix veratri (Radix Rhizoma Veratri) extract Thing, is used for killing acarina pest such as Tetranychus cinnabarinus, yellow tea mite, Panonychus citri etc..Patent documentation CN100444735C (Granted publication Number) disclose matrine, jervine, Flos Genkwa alkali insecticide.Patent documentation CN100411522C (Authorization Notice No.) discloses torr Product alkane Flos Daturae total alkali, nicotine, jervine are the parasite killing degermation of active component.Patent documentation CN105248435A discloses A kind of capillary and the synergistic activity biology compounded pesticides of jervine 20～1: 1 prevention and control of aphides.Patent documentation CN105191960A, CN105191962A, CN105165860A individually disclose the effective ingredient of 2.3% and are jervine and tears go out The water dispersible granules of prestige, the effective ingredient of 2% be the effective ingredient of jervine and the wettable powder of Aldicarb, 2.3% be Herba chenopodii The wettable powder of reed alkali and Aldicarb is used for preventing and treating Chinese rose red spider.Patent documentation CN104996437A disclose matrine, The insecticides such as nimbin, polyoxin, jervine, rotenone, nicotine.Patent documentation CN104855433A discloses one Plant jervine solution, kurarinone, Azedarachin, nicotine, double element alkali, derris root pyrethrin botanical pesticide.Patent documentation CN102246824A discloses the insecticides of a kind of muscardine and jervine preventing and treating poinsettia trialeurodes vaporariorum.Patent documentation CN103651435A discloses the insecticide of propylene glycol alginate, dibenzyl phenol polyethenoxy ether, jervine etc..Patent documentation CN104522054A discloses bacillus thuringiensis, jervine for preventing and treating Camellia sinensis tea geometrid, Euproctis pseudoconspersa, brassicaceous vegetable Many Species of Lepidopterous Insect Pests such as diamondback moth, Pieris rapae.Patent documentation CN102405917A discloses with jervine, cyromazine, auxiliary agent The insecticide of composition.Patent documentation CN104604945A discloses the Pesticidal combination containing green muscardine fungus and jervine.
Patent documents above is all to be used for preventing and treating plant insect by jervine and other combination of active principles, and by jervine For control of plant disease, and have no report with extract of willow bark composite usage in the technology of phytopathogen.
Based on above prior art, " Beijing City Education Commission's city-belonged university innovation ability promotes planning item " subsidize Complete the present invention.
Summary of the invention
It is an object of the invention to provide a kind of jervine plant source compound antibacterial, this antibacterial does not contains any chemical pesticide Composition, is prone to degraded in the environment, and to people, animal, Environmental security, pathogen is not likely to produce Drug resistance to it；Have simultaneously stable, Efficiently, the advantage of low cost.
Another object of the present invention is to provide the application of described jervine plant source compound antibacterial, be used for preventing and treating farming The brown rot of thing, gray mold, soft rot, scab, Bacterial septicaemia syndrome, downy mildew, phytophthora root rot, powdery mildew, late blight, dry rot The diseases such as disease.
For achieving the above object, the present invention is by the following technical solutions:
A kind of jervine plant source compound antibacterial, comprises jervine 0.1%-10%, bark of willow by weight percentage Extract 1-20%, remaining is auxiliary agent；Described jervine is that the sterling containing Rhizoma et radix veratri (Radix Rhizoma Veratri) total alkali, mixture or Veratrum extract Thing.
Described jervine plant source compound antibacterial is microemulsion, water preparation or wettable powder.
Wherein, described microemulsion is made up of following raw material: jervine 0.1%-10%, willow extract 1-20%, organic Solvent 0-20%, surfactant 5-10%, synergist 0-20%, antifreezing agent 0-5%, surplus is water.
Described water preparation is made up of following raw material: jervine 0.1%-10%, extract of willow bark 1-20%, surfactant 5-20%, synergist 0-20%, antifreezing agent 0-5%, preservative 1-3%, thickening agent 0.2-1%, surplus is water.
Described wettable powder is made up of following raw material: jervine 0.1%-10%, extract of willow bark 1-20%, potentiation Agent 0-20%, wetting agent 5-15%, dispersant 5-15%, surplus is carrier.
Described organic solvent is the one in ethyl acetate, methyl oleate, ethanol, methanol, acetone and dimethylformamide Or several mixture.
Described surfactant is alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ether The addition product of class, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, Oleum Ricini and oxirane and aralkyl phenol polyethenoxy The mixture of one or more in ether formaldehyde condensation products.
Described antifreezing agent is ethylene glycol or propylene glycol.
Described thickening agent be sodium carboxymethyl cellulose, propylene glycol alginate, carrageenan, xanthan gum, pectin, guar gum, The mixture of one or more in locust bean gum.
Described synergist is one or more in organosilicon, humic acid, Semen Sojae Preparatum polysaccharide.Described dispersant is alkylnaphthalene sulphur Barbiturates, pull powder Bx, sodium methylene bis-naphthalene sulfonate (NNO), wgwin D06 or dodecylbenzene sodium sulfonate.
Described carrier is active hargil, Kaolin or bentonite.
Described wetting agent is for reaching profit DCM-82, calcium lignosulfonate or sodium lignin sulfonate.
Described preservative is ethanol, calcium propionate and sodium lactate.
The present invention provides described antibacterial at preventing and treating crops brown rot, gray mold, soft rot, scab, bacillary wears Application in terms of the diseases such as hole disease, downy mildew, phytophthora root rot, powdery mildew, late blight, dry rot.
It is an advantage of the current invention that:
The antibacterial of the present invention does not contains any chemical pesticide composition, is prone to degraded in the environment, to people, animal, Environmental security, Pathogen is not likely to produce Drug resistance to it；There is stable, efficient, the advantage of low cost simultaneously.
The antibacterial of the present invention may be used for preventing and treating the brown rot of crops, gray mold, soft rot, scab, bacillary The diseases such as shothole disease, downy mildew, phytophthora root rot, powdery mildew, late blight, dry rot, it is also possible to for the preventing and treating of other plant disease.
Detailed description of the invention
Below by way of specific embodiment, the present invention will be further described.
Embodiment 1
20kg Rhizoma et radix veratri (Radix Rhizoma Veratri) (Veratrum nigrum L.) rhizome is purchased from Hebei Anguo Chinese Medicinal Materials Markets, sieves after pulverizing medicinal materials (16 mesh).The medical material pulverized, with 70% alcohol solvent continuous circumfluence extraction 6h, is spray-dried.Inhale with D101 macroporous adsorbent resin Attached, separate, 1-30% ethanol elution is reclaimed, be spray-dried, wherein jervine content is 2.0%.
Salix (VSalix babylonica) plant Ramulus Salicis Babylonicae skin, root bark pick up from the willow of Beijing area felling, are dried, Take 20kg grinding and sieving (16 mesh).The medical material pulverized, with water continuous circumfluence extraction 4h, is spray-dried and get final product, and 1g extract is suitable In 10g bark of willow.
Embodiment 2
The preparation of 0.1% (with jervine content meter, lower same) jervine+extract of willow bark wettable powder
Each raw material weighs by weight ratio, wherein, and jervine (2.0% raw material) 50g, extract of willow bark 100g, Semen Sojae Preparatum Polysaccharide 20g, calcium lignosulfonate 150g, calcium dodecyl benzene sulfonate 150g, active hargil 530g.Above-mentioned antibacterial wettable powder The preparation process of agent is: accurately weigh jervine and the former medicine of extract of willow bark, by the jervine weighed up and extract of willow bark Use Carriers Active bleaching earth adsorption, mix homogeneously, then the fermented soya beans, salted or other wise polysaccharide weighed, calcium dodecyl benzene sulfonate, calcium lignosulfonate are added Enter mix homogeneously, pulverize 35 minutes through super micron mill, cross 300 mesh sieves, obtain 0.1% jervine+extract of willow bark wettability Powder.
Embodiment 3
The preparation of 0.2% jervine+extract of willow bark wettable powder
Each raw material weighs by weight ratio, jervine (2% raw material) 100g, extract of willow bark 110g, humic acid 80g, Calcium lignosulfonate 100g, calcium dodecyl benzene sulfonate 100g, bentonite 510g.The preparation of above-mentioned antibacterial wettable powder Cheng Wei: accurately weigh jervine and the former medicine of extract of willow bark, by the jervine weighed up and extract of willow bark carrier swelling Soil absorption, mix homogeneously, then the humic acid weighed, dodecylbenzene sodium sulfonate, calcium lignosulfonate are added, mix homogeneously, Pulverize 30 minutes through super micron mill, cross 300 mesh sieves, obtain 0.2% jervine+extract of willow bark wettable powder.
Embodiment 4
The preparation of 0.3% jervine+extract of willow bark wettable powder
Each raw material weighs by weight ratio, jervine (2% raw material) 150g, extract of willow bark 88g, humic acid 50g, wood Quality sodium sulfonate 120g, pull powder Bx 120g, sodium acetate 2g, Kaolin 470g.The preparation of above-mentioned antibacterial wettable powder Cheng Wei: accurately weigh jervine and the former medicine of extract of willow bark, by the jervine weighed up and extract of willow bark carrier kaolinite Soil absorption, mix homogeneously, then the humic acid weighed, pull powder Bx, sodium lignin sulfonate are added mix homogeneously, through micronizing Machine is pulverized 30 minutes, crosses 300 mesh sieves, obtains 0.3% jervine+extract of willow bark wettable powder.
Embodiment 5
The preparation of 0.4% jervine+extract of willow bark microemulsion
At room temperature, count by weight, jervine (2% raw material) 200g, extract of willow bark 100g are mixed, then 150g organosilicon, 60g calcium dodecyl benzene sulfonate, 100g styrenated phenol polyoxyethylene it is sequentially added in said mixture (n30) ether, 50g ethylene glycol, water polishing to 1000g.Feed the mixture into mulser, mixing speed 280 revs/min, stir 30 Minute, obtain 0.4% jervine+extract of willow bark microemulsion.
Embodiment 6
The preparation of 0.25% jervine+extract of willow bark microemulsion
At room temperature, count by weight, jervine (2% raw material) 125g, extract of willow bark 50g are mixed, then 100g organosilicon, 100g calcium dodecyl benzene sulfonate, 80g styrenated phenol polyoxyethylene it is sequentially added in said mixture (n30) ether, 30mL ethylene glycol, 10mL 70% ethanol, water polishing to 1000g.Feed the mixture into mulser, mixing speed 300 Rev/min, stir 30 minutes, obtain 0.25% jervine+extract of willow bark microemulsion.
Embodiment 7
The preparation of 0.2% jervine+extract of willow bark microemulsion
At room temperature, count by weight, jervine (2% raw material) 100g, extract of willow bark 120g are mixed, then 50g humic acid, 70g dodecylbenzene sodium sulfonate, 120g Oleum Ricini, 20mL propylene glycol, 8mL it is sequentially added in said mixture 70% ethanol, water polishing to 1000g.Feed the mixture into mulser, mixing speed 300 revs/min, stir 30 minutes, to obtain final product 0.2% jervine+extract of willow bark microemulsion.
Embodiment 8
0.18% jervine+extract of willow bark water preparation preparation
At room temperature, count by weight, jervine (2% raw material) 90g, extract of willow bark 130g are mixed, upper State and mixture is sequentially added into Semen Sojae Preparatum polysaccharide 30g, 100g organosilicon, 90g dodecylbenzene sodium sulfonate, 30g ethanol, 10g xanthan Glue, solution polishing to 1000g, with mixing speed 260 revs/min, stirs 30 minutes, can be prepared by 0.18% jervine+willow Peel extract water preparation.
Embodiment 9
0.12% jervine+extract of willow bark water preparation preparation
At room temperature, count by weight, jervine (2% raw material) 60g, extract of willow bark 100g are mixed, upper State and mixture is sequentially added into humic acid 50g, 90g dodecylbenzene sodium sulfonate, 20mL 70% ethanol, 8g locust bean gum, solution Polishing is to 1000g, with mixing speed 300 revs/min, stirs 30 minutes, can be prepared by 0.12% jervine+extract of willow bark Water preparation.
Embodiment 10
0.2% jervine+extract of willow bark water preparation preparation
At room temperature, count by weight, jervine (2% raw material) 100g, extract of willow bark 150g are mixed, upper State and mixture is sequentially added into Semen Sojae Preparatum polysaccharide 80g, 50g dodecylbenzene sodium sulfonate, 15mL 70% ethanol, 9g locust bean gum, molten Liquid polishing is to 1000g, with mixing speed 270 revs/min, stirs 30 minutes, can be prepared by 0.2% jervine+bark of willow and extracts Thing water preparation.
Embodiment 11
The nine kinds of jervines+extract of willow bark source bactericide of gained and 0.4% jervine microemulsion in embodiment 2-10 (formula with embodiment 5, but without extract of willow bark) field control effectiveness test to peach brown rot
For the field efficacy situation of the most above-mentioned nine kinds of botanical fungicides, at Pinggu County, beijing, this medicament is prevented and treated The drug effect test of field zone of peach brown rot.Comparison medicament is 24% RH-7592 suspending agent, and test kind is Kubo, the age of tree 10 Year.With PB-16 type knapsack hand sprayer, every 667m2Water consumption 180-200kg.The 2500 times of liquid sprayings of RH-7592 suspension emulsion, 10% little jervine+extract of willow bark 1000 times liquid spraying, each concentration repeats 10 trees, and random district group arranges, and arranges sky White comparison (spray tap water 180-200kg/667m2)。
Application method: spray in June 25 medicine, dispenser 1 time in every 12 days, altogether dispenser 3 times.
Investigation method and data analysis: investigation in 10 days after first 20 days of last dispenser, dispenser, each tree divides east, south, west, north Sampling, takes fruit 80-110, adds up diseased fruit rate, calculate prevention effect.
Table 1 different agents prevention effect to Kubo peach brown rot
As shown in Table 1, when 0.4% jervine+extract of willow bark Emulsion dilutes 1000 times, preventive effect is 86.76%, When 0.4% jervine microemulsion dilutes 1000 times, preventive effect is 69.33%, far below 0.4% jervine+extract of willow bark Preventive effect, illustrates that jervine and extract of willow bark are compounding and can play synergism；The most also explanation is for the Rhizoma et radix veratri (Radix Rhizoma Veratri) of parasite killing Alkali has good preventive effect to brown rot germ.Synthesis result shows, 9 kinds of jervine+extract of willow bark preparations all can reach preferable Prevention effect, prevention effect is relevant with concentration, and concentration is the highest, and prevention effect is the best.
Embodiment 12
(formula is with real for nine kinds of jervines of embodiment 2-10+extract of willow bark source bactericide and 0.2% jervine water preparation agent Execute example 10, but without extract of willow bark) field control effectiveness test to botrytis cinerea
For the field efficacy situation of the most above-mentioned nine kinds of jervines+extract of willow bark source bactericide, in Luancheng pair, Hebei This medicament has carried out preventing and treating the drug effect test of field zone of botrytis cinerea.Test knapsack sprayer, plot experiment is Random alignment, is repeated 3 times, every plot area 0.5 mu, and kind is local bright red.Reagent agent dilutes 1000 times, carries out blade face normal Amount spraying (i.e. occurring on blade face that water membrane is advisable), is carried out with 1000 times of liquid of 40% pyrimethanil wettable powder for comparison medicament Blade face conventional spray, and set clear water blank.Investigate according to blank incidence classification.
Application method: spray in May 25 medicine, dispenser 1 time in every 12 days, altogether dispenser 3 times.
Use following stage division:
0 grade: anosis；
1 grade: lesion area accounts for less than the 5% of one-piece blade area；
3 grades: lesion area accounts for the 6%～15% of one-piece blade area；
5 grades: lesion area accounts for the 16～25% of one-piece blade area；
7 grades: lesion area accounts for the 26～50% of one-piece blade area；
9 grades: lesion area accounts for more than the 50% of one-piece blade area.
According to survey data, calculate disease index and the prevention effect of each process.Disease index is pressed formula (1) and is calculated, meter Calculate result and retain 2 significant digits.
In formula, X represents disease index；Ni represents the sick number of sheets at different levels；I represents relative level numerical value；N represents the total leaf of investigation.
Prevention effect is pressed formula (2) and is calculated:
P=100 × (CK-PT)/CK (2)
In formula, P represents prevention effect (%)；CK represents blank disease index；PT represents chemicals treatment disease index.
Table 2 different agents field control effectiveness test result to graw mold of tomato
Process group Disease index Prevention effect %
Blank 38.21 0.00
0.1% jervine+extract of willow bark wettable powder 16.63 56.48
0.2% jervine+extract of willow bark wettable powder 10.67 72.08
0.3% jervine+extract of willow bark wettable powder 8.84 76.86
0.4% jervine+extract of willow bark microemulsion 6.37 83.33
0.25% jervine+extract of willow bark microemulsion 11.55 69.77
0.2% jervine+extract of willow bark microemulsion 8.92 76.66
0.18% jervine+extract of willow bark water preparation 15.7 58.47
0.12% jervine+extract of willow bark water preparation 10.6 71.84
0.2% jervine+extract of willow bark water preparation 7.23 81.08
0.2% jervine water preparation 10.16 73.41
40% pyrimethanil wettable powder 8.12 78.75
As shown in Table 2, when 0.2% jervine+extract of willow bark water preparation dilutes 1000 times, preventive effect is 81.08%, When 0.2% jervine water preparation dilutes 1000 times, preventive effect is 73.41%, less than 0.2% jervine+extract of willow bark water preparation Preventive effect, illustrates that jervine and extract of willow bark are compounding and graw mold of tomato is played synergism；The most also explanation is used for The jervine of parasite killing is used alone also favorable effect to graw mold of tomato.Synthesis result shows, 9 kinds of jervines in embodiment + extract of willow bark preparation all can reach preferable prevention effect, and prevention effect is relevant with concentration, and concentration is the highest, prevention effect The best.

Claims (10)

1. a jervine plant source compound antibacterial, it is characterised in that: comprise jervine 0.1%-by weight percentage 10%, extract of willow bark 1-20%, remaining is auxiliary agent；Described jervine is the sterling containing Rhizoma et radix veratri (Radix Rhizoma Veratri) total alkali, mixture or Rhizoma et radix veratri (Radix Rhizoma Veratri) Genus plants extract.
Jervine plant source compound antibacterial the most according to claim 1, it is characterised in that: described jervine plant source is multiple Joining antibacterial is microemulsion, water preparation or wettable powder.
Jervine plant source compound microemulsion agent the most according to claim 2, it is characterised in that: described microemulsion is by following Raw material is made: jervine 0.1%-10%, willow extract 1-20%, organic solvent 0-20%, surfactant 5-10%, increases Effect agent 0-20%, antifreezing agent 0-5%, surplus is water.
Jervine plant source compound microemulsion the most according to claim 3, it is characterised in that: described organic solvent is acetic acid The mixture of one or more in ethyl ester, methyl oleate, ethanol, methanol, acetone and dimethylformamide；Described surface activity Agent is alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether One in the addition product of polyoxypropylene ethers, Oleum Ricini and oxirane and aralkyl phenol polyethenoxy ether formaldehyde condensation products or Several mixture；Described synergist is one or more in organosilicon, humic acid, Semen Sojae Preparatum polysaccharide；Described antifreezing agent is second Glycol or propylene glycol.
Jervine plant source compound water preparation the most according to claim 2, it is characterised in that: described water preparation is by following raw material system Become: jervine 0.1%-10%, extract of willow bark 1-20%, surfactant 5-20%, synergist 0-20%, antifreezing agent 0- 5%, preservative 1-3%, thickening agent 0.2-1%, surplus is water.
6. a right is according to the jervine plant source compound water preparation required described in 5, it is characterised in that: described surfactant is alkyl Benzenesulfonates, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene Ethers, Oleum Ricini are mixed with one or more in the addition product of oxirane and aralkyl phenol polyethenoxy ether formaldehyde condensation products Compound；Described synergist is one or more in organosilicon, humic acid, Semen Sojae Preparatum polysaccharide；Described antifreezing agent is ethylene glycol or third Glycol；Described thickening agent is sodium carboxymethyl cellulose, propylene glycol alginate, carrageenan, xanthan gum, pectin, guar gum, Robinia pseudoacacia L. The mixture of one or more in locust beam gum；Described preservative is ethanol, calcium propionate and sodium lactate.
Jervine plant source compound wettable powder the most according to claim 2, it is characterised in that: described wettable powder Be made up of following raw material: jervine 0.1%-10%, extract of willow bark 1-20%, synergist 0-20%, wetting agent 5-15%, Dispersant 5-15%, surplus is carrier.
Jervine plant source compound wettable powder the most according to claim 7, it is characterised in that: described synergist is for having One or more in machine silicon, humic acid, Semen Sojae Preparatum polysaccharide；Described wetting agent is for reaching profit DCM-82, calcium lignosulfonate or wooden Element sodium sulfonate；Described dispersant is alkyl naphthalene sulfonic acid salt, pull powder Bx, NNO, wgwin D06 or dodecylbenzene sodium sulfonate； Described carrier is active hargil, Kaolin or bentonite.
9. the jervine plant source compound antibacterial according to any one of claim 1-8 is in terms of preventing and treating corps diseases Application.
Application the most according to claim 9, it is characterised in that: described disease is brown rot, gray mold, soft rot, black star One or more in disease, Bacterial septicaemia syndrome, downy mildew, phytophthora root rot, powdery mildew, late blight and dry rot.
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CN109156286A (en) * 2018-09-04 2019-01-08 横县校椅供销卫民种养专业合作社联合社 A kind of greenhouse gardening method of sweet No. 12 muskmelons in osmanthus
CN109892342A (en) * 2019-02-12 2019-06-18 安徽农业大学 Biocontrol agent associated with a kind of trichothecium roseum and jervine and its application in corn southern leaf blight prevention and treatment
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