CN106508993A - Pesticide resistant to tobacco mosaic virus and preparation method of pesticide - Google Patents
Pesticide resistant to tobacco mosaic virus and preparation method of pesticide Download PDFInfo
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- CN106508993A CN106508993A CN201610926322.8A CN201610926322A CN106508993A CN 106508993 A CN106508993 A CN 106508993A CN 201610926322 A CN201610926322 A CN 201610926322A CN 106508993 A CN106508993 A CN 106508993A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention provides a pesticide resistant to a tobacco mosaic virus and belongs to the technical field of pesticides. The pesticide resistant to the tobacco mosaic virus is prepared from the following raw materials including, by weight, 10-30 parts of sophora flavescens, 10-30 parts of semen aesculi, 10-30 parts of mugwort, 5-20 parts of litchi hulls, 5-20 parts of lemon peels, 5-20 parts of loofah peels, 5-15 parts of cinnamomum camphora leaves, 3-12 parts of rosemary, 3-12 parts of forsythia suspense, 3-12 parts of acorus calamus and 1-5 parts of biopreparate. The biopreparate is composed of, by mass, 1-10% of bacillus thuringiensis, 30-40% of streptomyces microflavus and 50-60% of photosynthetic bacteria. The materials of the pesticide are environmentally friendly, the pesticide is harmless to tobacco and plants and capable of efficiently killing the tobacco mosaic virus, the virus resistant rate reaches 90% or above, and the pesticide resistance is avoided during long-time using.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of pesticide of resisting tobacco mosaic virus and preparation method thereof.
Background technology
Tobacco mosaic virus (TMV)（TMV）It is a kind of plant viruses of serious harm tobacco leaf production, after the Tobacco Infected virus, children
Leaflet tablet lateral vein and offshoot organize the shape that is translucent, and vein both sides mesophyll tissue is in gradually light green.Virus is big in leaf tissue
Amount propagation, makes the increase of part mesophyll cell or increases, and occurs that blade thin and thick is irregular, and color is yellowish green alternate, in floral leaf shape.Floral leaf afterwards
Mottled degree is increased, and existing large area dark brown ecthyma gangrenosa, middle and lower part old leaf more so, the leaf-shrinkage of weight of falling ill, deformity, torsion
It is bent.The plant shortened internodes of early stage morbidity, serious to downgrade, poor growth, it is impossible to normally blossom and bear fruit, and it is easy to fall off, can develop
Shady fruit is little and shrinkage, grain weight is few and little, can not germinate more.Tobacco mosaic disease is most easy in tobacco in seedling stage and grown in field initial stage
It is susceptible, the seedbed phase is occurred mainly in land for growing field crops squaring period.
Nicotiana tabacum L. drafts a document eggplant wood, and Solanaceae is annual or limited herbaceos perennial, base portion slightly lignifying.Inflorescence basidixed, circle
Taper, spends more；Capsule ovate or square round shape, length are approximately equal to persistent calyx.Summer and autumn yields positive results.It is distributed mainly on South America, south
Sub-, China.Nicotiana tabacum L. decapacitation is made Medicated cigarette, tobacco, pipe tobacco, cigar etc. and sucks outer for people, still has various medical applications.Although cigarette
Grass gives people many harm of class band, or even is referred to as " poisonous weeds ", the restricted circulation of many country plaintexts or suction, and the world defends
Raw organizational member also endorsed《The Framework Convention on Tobacco Control》.But as a kind of time-honored medicinal plants, its medical value
Can not be obliterated because of its hazardness.《National Chinese herbal medicine compilation》Record, Nicotiana tabacum L. sweet in the mouth warm in nature is poisonous, with detumescence, removing toxic substances,
The effects such as parasite killing, it is mainly used in the diseases such as furuncle swelling toxin, tinea capitis, tinea alba, tinea alba, venom, can also treats a cellulitis, carbuncle on the back, wind
Expectorant, crane knee joint（Including bone tuberculosis, chronic purulent gonarthritis etc.）Deng disease.The generation of tobacco mosaic disease is caused to tobacco leaf production
Very big economic loss.Therefore, it is the important measures for ensureing Nicotiana tabacum L. good quality and high output to prevent and treat Nicotiana tabacum L. pest and disease damage.
The preparation and method for being presently used for resisting tobacco mosaic virus has a lot, for example, Application No. 201110160947.5
Patent disclose a kind of Schizophyllum commune Fr polysaccharides extract and preparation method thereof, Schizophyllum commune Franch． mycelium powder is added to into hot water proportionally
In, extract 8 ~ 10 hours in 100 DEG C of water-baths, with filtered through gauze, gained filtering residue is being extracted once by the same way again, is merged
Filtrate, concentrated in vacuo twice, then mixes with 95% ethanol of 5 times of volumes, stands overnight at 4 DEG C, and centrifugation, by precipitation ethanol
Washing 2 times, 60 DEG C of drying.The Schizophyllum commune Fr polysaccharides extract of the invention can be used to prevent and treat tobacco mosaic virus disease, to Nicotiana tabacum L.
The suppression ratio of mosaic viruss can reach more than 70%, and be natural extract, to the mankind and environmentally friendly.Application No.
201510836720.6 patent discloses the preparation method that a kind of preventing and treating tobacco mosaic virus (TMV) Tobacco mosaic that goes out.Its strain group
It is divided into：Photosynthetic bacteria 5%, aspergillus niger 2%, thin Huang streptomyces microflavus 3%, Paecilomyces lilacinus 2%, bacillus thuringiensiss 15%, colloid bud
Spore bacillus 15%, bacillus megaterium 15%, bacillus polymyxa 10%, Bacillus licheniformis 10%, green muscardine funguss 3%, wax-like spore bar
Bacterium 15%, Brevibacillus laterosporus 5%, the present invention are combined from multiple beneficial bacterium, and substantially, prevention effect is up to more than 85% for preventive effect.
Technology disclosed above is significantly different using the method for chemicals with tradition, more safe and environment-friendly, green, but prevention effect
And stability is for traditional chemical agents, it need further to improve.
The content of the invention
In view of this, the technical problem to be solved is to provide a kind of pesticide of resisting tobacco mosaic virus, antibacterial
Effect stability, strong drug action and environmentally safe, pollution-free.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：10 ~ 30 parts of Radix Sophorae Flavescentiss, Semen Aesculi 10 ~
30 parts, 10 ~ 30 parts of Radix Artemisia ordosicae, 5 ~ 20 parts of Exocarpium Litchi, 5 ~ 20 parts of Fericarpium Citri Limoniae, 5 ~ 20 parts of Cortex Luffae, 5 ~ 15 parts of Folium Cinnamomi porrecti, Herba Rosmarini Officinalis 3 ~
12 parts, 3 ~ 12 parts of Fructus Forsythiae, 3 ~ 12 parts of Rhizoma Acori Graminei, 1 ~ 5 part of bacteria agent, 1 ~ 10% Su Yun of the bacteria agent by mass percent
Golden bacillus cereuss, 30 ~ 40% streptomyces microflavus and 50 ~ 60% photosynthetic bacterias composition.
Preferably, 8% bacillus thuringiensiss, 35% streptomyces microflavus and 57% of the bacteria agent by mass percent
Photosynthetic bacteria constitutes.
Preferably, the pesticide of the resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：Radix Sophorae Flavescentiss 20 ~ 25
Part, 20 ~ 25 parts of Semen Aesculi, 15 ~ 20 parts of Radix Artemisia ordosicae, 8 ~ 12 parts of Exocarpium Litchi, 8 ~ 12 parts of Fericarpium Citri Limoniae, 8 ~ 12 parts of Cortex Luffae, Folium Cinnamomi porrecti 8 ~
12 parts, 9 parts of Herba Rosmarini Officinalis, 9 parts of Fructus Forsythiae, 9 parts of Rhizoma Acori Graminei, 2 ~ 3 parts of bacteria agent.
Preferably, the pesticide of the resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：20 parts of Radix Sophorae Flavescentiss,
23 parts of Semen Aesculi, 18 parts of Radix Artemisia ordosicae, 10 parts of Exocarpium Litchi, 10 parts of Fericarpium Citri Limoniae, 10 parts of Cortex Luffae, 10 parts of Folium Cinnamomi porrecti, 9 parts of Herba Rosmarini Officinalis, company
Stick up 9 parts, 9 parts of Rhizoma Acori Graminei, 2 parts of bacteria agent.
A kind of preparation method of the pesticide of resisting tobacco mosaic virus, comprises the following steps：
1）10 ~ 30 parts of the Radix Sophorae Flavescentiss of dry weight portion, 10 ~ 30 parts of Semen Aesculi, 10 ~ 30 parts of Radix Artemisia ordosicae, Exocarpium Litchi 5 ~ 20 is weighed respectively
3 ~ 12 parts of part, 5 ~ 20 parts of Cortex Luffae, 3 ~ 12 parts of Herba Rosmarini Officinalis, 3 ~ 12 parts of Fructus Forsythiae and Rhizoma Acori Graminei, mixing enter after freezing under nitrogen protection
Row is crushed, and obtains comminuting matter I；5 ~ 15 parts of 5 ~ 20 parts of the Fericarpium Citri Limoniae and Folium Cinnamomi porrecti of dry weight portion, mixing, powder is weighed respectively
It is broken, obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and medicinal residues I, filtrate is cooled down, sealing preserve；The water of 2 ~ 3 times of its weight is added in the medicinal residues I, is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filtered while hot obtains filtrate II and medicinal residues II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 extractum, in the extractum adds volume fraction
For 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, kept for 18 ~ 24 hours, then filter, obtain filtrate III and precipitate, by institute
State the mastic that relative density when filtrate III is evaporated to 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, in adding distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and extracting solution is closed by distillation extraction 2 ~ 3 times
And extracted with organic solvent, sub-cloud solution, vacuum revolving instrument steams organic solvent, obtains final product extract；
4）The mastic and extract are separately dried, are crushed, merged, obtain effective ingredient I；
5）The effective ingredient I is mixed with 1 ~ 5 weight portion of bacteria agent, 2000 ~ 3000rpm is stirred 5 ~ 10 minutes, is obtained final product.
Preferably, the w/v 1g of the extractum and the ethanol：10~20ml.
Preferably, the organic solvent is dichloromethane.
Compared with prior art, its advantage is as follows for the present invention：
1）Present invention applicant is on the basis of studying to tobacco planting, it is proposed that a kind of pesticide of resisting tobacco mosaic virus, should
Pesticide is drawn materials environmental protection, to Nicotiana tabacum L. and plant without any harm, and can efficiently kill tobacco mosaic virus (TMV), and disease-resistant rate reaches
More than 90%, life-time service is without Drug resistance.
2）The raw materials used wide material sources of the present invention, safety and environmental protection.Dry root of the Radix Sophorae Flavescentiss for leguminous plant Radix Sophorae Flavescentiss, heat clearing away are dry
It is wet, parasite killing.Dry mature seed of the Semen Aesculi for Aesculus, surface brown or sepia, many shrinkages are uneven, somewhat light
Pool.Radix Artemisia ordosicae, is perennial herb or slightly into suffruticose plant, and plant has enhanced aroma, with antibacterial and antivirus action, flat
Breathe heavily, antitussive and phlegm-dispelling functions, hemostasis and blood coagulation resisting function etc..Exocarpium Litchi, is the peel of sapindaceous plant Fructus Litchi, nature and flavor are bitter,
It is cold, with dehumidifying dysentery relieving, anastalsis；Containing tens kinds of compositions such as Flavonoid substances in litchi rind.Fericarpium Citri Limoniae, is that Rutaceae is planted
Thing Canton lemon or the peel of lemon, gas are fragrant, mildly bitter flavor, and extract contains various bacteriostatic active ingredients.Cortex Luffae, is the fruit of Fructus Luffae
Skin, eating can heat-clearing and toxic substances removing.Folium Cinnamomi porrecti, is the fresh leaf of Cinnamomum porrectum (Roxb.) Kosterm., and mainly containing volatile oil component, wherein 1, 8-Cineole content is about
60%th, Camphora about 0.75%, other compositions have citral, capraldehyde, australene, camphene, α-terpinene, cymol, Lignum cinnamomi camphorae
Alcohol, alpha-terpineol and benzyl system sesquiterpene etc..Herba Rosmarini Officinalis, be labiate Herba Rosmarini Officinalis herb, contained volatile oil is to golden yellow
Staphylococcuses, escherichia coli, vibrio cholera etc. have certain antibacterial action.Fructus Forsythiae, also known as Hemerocallis citrina Baroni bar, Fructus Forsythiae, green grass or young crops stick up, fall to sticking up,
Huang Qidan etc., containing Neolignans, flavone compound, styrenic derivatives etc., has antibacterial, heart tonifying, diuresis, town to tell
Deng pharmacological action.Rhizoma Acori Graminei, can for ward off it is dirty have one's ideas straightened out, a surname's gas by expectorant, removing toxic substances, parasite killing.Above-mentioned raw materials be applicant through research for many years,
Screening, through science compounding gained；And be used in combination with bacteria agent, tonification each other, the whole growth cycle of Nicotiana tabacum L. is sent out
Raw tobacco mosaic virus (TMV) has obvious prevention effect, after the absorbable utilization of Nicotiana tabacum L., is conducive to the raising of quality of tobacco, and this
Invention pesticide does not have any injury to non-target object.
Specific embodiment
For a better understanding of the present invention, present disclosure, but this are further fairly set out with reference to embodiment
Bright protection content is not limited solely to the following examples.In the following description, a large amount of concrete details are given so as to
More thorough understanding of the invention is provided.It will be apparent, however, to one skilled in the art that the present invention can be with
It is carried out without the need for one or more of these details.
Embodiment 1：A kind of pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：20 parts of Radix Sophorae Flavescentiss,
23 parts of Semen Aesculi, 18 parts of Radix Artemisia ordosicae, 10 parts of Exocarpium Litchi, 10 parts of Fericarpium Citri Limoniae, 10 parts of Cortex Luffae, 10 parts of Folium Cinnamomi porrecti, 9 parts of Herba Rosmarini Officinalis, company
Stick up 9 parts, 9 parts of Rhizoma Acori Graminei, 2 parts of bacteria agent, 8% bacillus thuringiensiss, 35% thin yellow of the bacteria agent by mass percent
Streptomycete and 57% photosynthetic bacteria composition.
Bacillus thuringiensiss, Bacillus mucilaginosus, living bacteria count are 5 ~ 1,000,000,000 cfu/g；Thin Huang chain
Mycete, Streptomyces microflavus, living bacteria count >=200,000,000/g；Photosynthetic bacteria, living bacteria count are 50 ~ 100
Hundred million/g.Above-mentioned microbial inoculum can be obtained by market channel purchase, or is obtained by conventional method fermentation.
The preparation method of the pesticide of the present embodiment resisting tobacco mosaic virus, comprises the following steps：
1）Weigh respectively dry above-mentioned Radix Sophorae Flavescentiss, Semen Aesculi, Radix Artemisia ordosicae, Exocarpium Litchi, Cortex Luffae, Herba Rosmarini Officinalis, Fructus Forsythiae and Rhizoma Acori Graminei, mix
Close, crushed after freezing under nitrogen protection, obtain comminuting matter I；Dry above-mentioned Fericarpium Citri Limoniae and Folium Cinnamomi porrecti is weighed respectively,
Mixing, crushes, and obtains comminuting matter II；
2）The water of 5 times of its weight being added in the comminuting matter I, 50 DEG C are heated to, being kept for 45 minutes, filtered while hot is obtained
Filtrate I and medicinal residues I, filtrate are cooled down, sealing preserve；The water of 2 times of its weight is added in the medicinal residues I, 55 DEG C are heated to, is kept
35 minutes, filtered while hot obtained filtrate II and medicinal residues II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.13 extractum, adds the ethanol solution that volume fraction is 75%, temperature in the extractum
28 DEG C, kept for 24 hours, then filter, obtain filtrate III and precipitate, it is relative when the filtrate III is evaporated to 55 DEG C
Density is 1.23 mastic；
3）The water of its 4 times of weight of weight being added in the comminuting matter II, in adding distilling flask, being distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 38 minutes, and distillation extraction 2 times, by extracting solution merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The mastic and extract are separately dried, are crushed, merged, obtain effective ingredient I；
5）The effective ingredient I is mixed with 2 weight portion of bacteria agent, 2500rpm is stirred 10 minutes, is obtained final product.
The extractum is 1g with the w/v of the ethanol：16ml.
The using method of the pesticide of the resisting tobacco mosaic virus is：Add the pesticide weight of the resisting tobacco mosaic virus
More than 100 times of water, is thoroughly mixed, and makes spray, is uniformly sprayed at tobacco leaf surface.
Embodiment 2：A kind of pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：25 parts of Radix Sophorae Flavescentiss,
25 parts of Semen Aesculi, 15 parts of Radix Artemisia ordosicae, 12 parts of Exocarpium Litchi, 8 parts of Fericarpium Citri Limoniae, 12 parts of Cortex Luffae, 12 parts of Folium Cinnamomi porrecti, 9 parts of Herba Rosmarini Officinalis, Fructus Forsythiae
9 parts, 9 parts of Rhizoma Acori Graminei, 3 parts of bacteria agent, 8% bacillus thuringiensiss, 35% thin Huang chain of the bacteria agent by mass percent
Mycete and 57% photosynthetic bacteria composition.
The preparation method of the pesticide of the present embodiment resisting tobacco mosaic virus, comprises the following steps：
1）Weigh respectively the Radix Sophorae Flavescentiss of dry above-mentioned weight, Semen Aesculi, Radix Artemisia ordosicae, Exocarpium Litchi, Cortex Luffae, Herba Rosmarini Officinalis, even
Stick up and Rhizoma Acori Graminei, mix, crushed after freezing under nitrogen protection, obtain comminuting matter I；Dry above-mentioned weight is weighed respectively
The Fericarpium Citri Limoniae and Folium Cinnamomi porrecti of part proportioning, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 times of its weight being added in the comminuting matter I, 40 DEG C are heated to, being kept for 45 minutes, filtered while hot is obtained
Filtrate I and medicinal residues I, filtrate are cooled down, sealing preserve；The water of 2 times of its weight is added in the medicinal residues I, 50 DEG C are heated to, is kept
38 minutes, filtered while hot obtained filtrate II and medicinal residues II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.15 extractum, adds the ethanol solution that volume fraction is 80%, temperature in the extractum
25 DEG C, kept for 24 hours, then filter, obtain filtrate III and precipitate, it is relative when the filtrate III is evaporated to 55 DEG C
Density is 1.25 mastic；
3）The water of its 3 times of weight of weight being added in the comminuting matter II, in adding distilling flask, being distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 35 minutes, and distillation extraction 2 times, by extracting solution merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The mastic and extract are separately dried, are crushed, merged, obtain effective ingredient I；
5）The effective ingredient I is mixed with 3 weight portion of bacteria agent, 2000rpm is stirred 8 minutes, is obtained final product.
The extractum is 1g with the w/v of the ethanol：12ml.
The using method of the pesticide of the resisting tobacco mosaic virus is repeated no more with embodiment 1.
Embodiment 3：A kind of pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：22 parts of Radix Sophorae Flavescentiss,
20 parts of Semen Aesculi, 20 parts of Radix Artemisia ordosicae, 8 parts of Exocarpium Litchi, 12 parts of Fericarpium Citri Limoniae, 8 parts of Cortex Luffae, 8 parts of Folium Cinnamomi porrecti, 9 parts of Herba Rosmarini Officinalis, Fructus Forsythiae 9
Part, 9 parts of Rhizoma Acori Graminei, 2 parts of bacteria agent, 8% bacillus thuringiensiss, 35% thin Huang strepto- of the bacteria agent by mass percent
Bacterium and 57% photosynthetic bacteria composition.
The preparation method of the pesticide of the present embodiment resisting tobacco mosaic virus, comprises the following steps：
1）Weigh respectively the Radix Sophorae Flavescentiss of dry above-mentioned weight, Semen Aesculi, Radix Artemisia ordosicae, Exocarpium Litchi, Cortex Luffae, Herba Rosmarini Officinalis, even
Stick up and Rhizoma Acori Graminei, mix, crushed after freezing under nitrogen protection, obtain comminuting matter I；Dry above-mentioned weight is weighed respectively
The Fericarpium Citri Limoniae and Folium Cinnamomi porrecti of part proportioning, mixing are crushed, and obtain comminuting matter II；
2）The water of 6 times of its weight being added in the comminuting matter I, 50 DEG C are heated to, being kept for 45 minutes, filtered while hot is obtained
Filtrate I and medicinal residues I, filtrate are cooled down, sealing preserve；The water of 3 times of its weight is added in the medicinal residues I, 60 DEG C are heated to, is kept
40 minutes, filtered while hot obtained filtrate II and medicinal residues II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.12 extractum, adds the ethanol solution that volume fraction is 75%, temperature in the extractum
30 DEG C, kept for 20 hours, then filter, obtain filtrate III and precipitate, it is relative when the filtrate III is evaporated to 55 DEG C
Density is 1.21 mastic；
3）The water of its 5 times of weight of weight being added in the comminuting matter II, in adding distilling flask, being distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 40 minutes, and distillation extraction 3 times, by extracting solution merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The mastic and extract are separately dried, are crushed, merged, obtain effective ingredient I；
5）The effective ingredient I is mixed with 2 weight portion of bacteria agent, 3000rpm is stirred 8 minutes, is obtained final product.
The extractum is 1g with the w/v of the ethanol：18ml.
The using method of the pesticide of the resisting tobacco mosaic virus is repeated no more with embodiment 1.
Embodiment 4：A kind of pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：10 parts of Radix Sophorae Flavescentiss,
10 parts of Semen Aesculi, 30 parts of Radix Artemisia ordosicae, 5 parts of Exocarpium Litchi, 5 parts of Fericarpium Citri Limoniae, 5 parts of Cortex Luffae, 15 parts of Folium Cinnamomi porrecti, 12 parts of Herba Rosmarini Officinalis, Fructus Forsythiae
12 parts, 3 parts of Rhizoma Acori Graminei, 1 part of bacteria agent, 10% bacillus thuringiensiss, 40% thin yellow of the bacteria agent by mass percent
Streptomycete and 50% photosynthetic bacteria composition.
In the present embodiment, the preparation method and using method of the pesticide of resisting tobacco mosaic virus refers to embodiment 1, no longer goes to live in the household of one's in-laws on getting married
State.
Embodiment 5：A kind of pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：10 parts of Radix Sophorae Flavescentiss,
30 parts of Semen Aesculi, 10 parts of Radix Artemisia ordosicae, 20 parts of Exocarpium Litchi, 20 parts of Fericarpium Citri Limoniae, 20 parts of Cortex Luffae, 5 parts of Folium Cinnamomi porrecti, 3 parts of Herba Rosmarini Officinalis, Fructus Forsythiae
3 parts, 12 parts of Rhizoma Acori Graminei, 5 parts of bacteria agent, 5% bacillus thuringiensiss, 35% thin Huang chain of the bacteria agent by mass percent
Mycete and 60% photosynthetic bacteria composition.
In the present embodiment, the preparation method and using method of the pesticide of resisting tobacco mosaic virus refers to embodiment 1, no longer goes to live in the household of one's in-laws on getting married
State.
Embodiment 6：A kind of pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：10 parts of Radix Sophorae Flavescentiss,
15 parts of Semen Aesculi, 25 parts of Radix Artemisia ordosicae, 15 parts of Exocarpium Litchi, 15 parts of Fericarpium Citri Limoniae, 15 parts of Cortex Luffae, 6 parts of Folium Cinnamomi porrecti, 6 parts of Herba Rosmarini Officinalis, Fructus Forsythiae
6 parts, 10 parts of Rhizoma Acori Graminei, 4 parts of bacteria agent, 1% bacillus thuringiensiss, 40% thin Huang chain of the bacteria agent by mass percent
Mycete and 59% photosynthetic bacteria composition.
In the present embodiment, the preparation method and using method of the pesticide of resisting tobacco mosaic virus refers to embodiment 1, no longer goes to live in the household of one's in-laws on getting married
State.
Embodiment 7：The pesticide of the resisting tobacco mosaic virus described by the present embodiment, as different from Example 1：
Also include：1 weight portion of auxiliary agent, the auxiliary agent are to compare 3 by spaonin powder, polyvinyl alcohol, sodium citrate with weight：2：1.2 group
Into mixture.
Wherein, spaonin powder be protophyte Fructus Gleditsia Jing retrofits, grinding form, with good dispersion, moistening, suspension
Etc. function.Polyvinyl alcohol, pulverulent solids, for pesticide, can improved soil or have potentiation.Sodium citrate, white are arrived
Clear crystal, with good complex performance.Above-mentioned three kinds of auxiliary agents are shared, and can strengthen the performance of pesticide efficacy, improve pesticide
Resistances against diseases.
Embodiment 8：The pesticide of the resisting tobacco mosaic virus described by the present embodiment, with embodiment 1 except that：
Also include：2 weight portion of auxiliary agent, the auxiliary agent are to compare 2 by spaonin powder, polyvinyl alcohol, sodium citrate with weight：1：0.8 group
Into mixture.
Comparative example 1：The pesticide of resisting tobacco mosaic virus, is prepared from by the raw material of following weight portion：20 parts of Radix Sophorae Flavescentiss, sieve Suo
23 parts of son, 18 parts of Radix Artemisia ordosicae, 10 parts of Exocarpium Litchi, 10 parts of Fericarpium Citri Limoniae, 10 parts of Cortex Luffae, 10 parts of Folium Cinnamomi porrecti, 9 parts of Herba Rosmarini Officinalis, Fructus Forsythiae 9
Part, 9 parts of Rhizoma Acori Graminei.
Comparative example 2：The pesticide of resisting tobacco mosaic virus, is prepared from by the microbial bacterial agent of following mass percent：8%
Bacillus thuringiensiss, 35% streptomyces microflavus and 57% photosynthetic bacteria.
Comparative example 3：The pesticide of resisting tobacco mosaic virus, with embodiment 1 except that：Also include：2 weight portion of auxiliary agent,
The auxiliary agent is to compare 1 by spaonin powder, polyvinyl alcohol, sodium citrate with weight：1：The mixture of 1 composition.
Effect assessment
1.1 reagent agent
Experimental group：ⅠA~ⅠF：It is followed successively by the pesticide of the resisting tobacco mosaic virus of the preparation of the embodiment of the present invention 1 ~ 6；
ⅠG~ⅠH：It is followed successively by the pesticide of the resisting tobacco mosaic virus of the preparation of the embodiment of the present invention 7 ~ 8；
Matched group：ⅡA：The pesticide of resisting tobacco mosaic virus prepared by comparative example 1；
ⅡB：The pesticide of resisting tobacco mosaic virus prepared by comparative example 2；
ⅡC：The pesticide of resisting tobacco mosaic virus prepared by comparative example 3.
1.2 inhibitory activity are tested
1）Assay method
For trying pathogenic bacteria：Tobacco mosaic virus (TMV)（Tobacco mosaic virus）.
Test plant：Withered spot host's Nicotiana glutinosa.By Nicotiana glutinosa seed first with the 30min that hots water treatment of seeds during nursery.Then will plant
Son is directly sowed in seedling-growing container, puts it into illumination box, and 22 DEG C of temperature, illumination 16h/d, humidity 60% are cultivated, when
Nicotiana tabacum L. supplies examination when growing to 5 ~ 6 true leaves.
Inoculation：It is inoculated with using juice friction；
From 5 ~ 6 leaf phase Nicotiana glutinosas as the withered spot host for supplying examination pathogenic bacteria；
Test is carried out in insect prevention greenhouse.Reagent agent is taken, 0.05g/ml aqueous solutions is configured to distilled water, add for examination disease
Bacterium, makes the final concentration of 10 μ g/ml of virus.Mixing 10min, it is left using half leaf method frictional inoculation in withered spot host Nicotiana glutinosa（It is right）Half
Ye Shang, separately with the distilled water containing TMV viruses（The final concentration of 10 μ g/ml of virus）For control, 5 leaves of inoculation, test weight is often processed
It is multiple 3 times.After 3 days after obvious withered spot to appear, record withered spot number, calculates suppression ratio in time（%）, commented with average inhibition
Valency inhibition；
Suppression ratio=（Control withered spot number-process withered spot number）/ control withered spot number × 100.
1.3 bacteriostatic activity
1 reagent agent of table is to supplying to try the suppression ratio of pathogenic bacteria
| Group | ⅠA | ⅠB | ⅠC | ⅠD | ⅠE | ⅠF | ⅠG | ⅠH | ⅡA | ⅡB | ⅡC |
| For trying pathogenic bacteria/suppression ratio, % | 98.2 | 97.6 | 97.1 | 95.7 | 94.6 | 96.1 | 99.5 | 99.1 | 39.2 | 45.6 | 85.9 |
As it can be seen from table 1 the pesticide of the resisting tobacco mosaic virus prepared by the embodiment of the present invention 1 ~ 6 is to tobacco mosaic virus (TMV)
Suppression ratio is 94.6 ~ 98.2%, is above 90%, and suppression ratio fluctuates little, efficacy stability, and apparently higher than comparative example 1 and 2, increases
Effect is obvious.Additionally, the antiviral effect of group I G is superior to I A ~ I F, I H is essentially identical with I G, and two groups to tobacco mosaic virus (TMV)
Inhibition all strengthened, can be used as more preferred scheme.The suppression ratio of comparative example 3 is 85.9%, compared with embodiment 7
For, hence it is evident that reduce, illustrate that performance of the rational proportion of auxiliary agent to pesticide efficacy of the present invention has important impact, while saying
Bright, auxiliary agent of the present invention can improve the anti-virus ability of pesticide of the present invention.
Above-mentioned experiment shows that pesticide of the present invention is used for the preventing and treating of tobacco mosaic virus (TMV) and imitates with notable and stable suppression
Really, strong drug action, potentiation are obvious.
Although the foregoing describing the specific embodiment of the present invention, those familiar with the art should manage
Solution, the specific embodiment described by us are merely exemplary, rather than for the restriction to the scope of the present invention, are familiar with this
The technical staff in field should be covered the present invention's in the equivalent modification and change made according to the spirit of the present invention
In scope of the claimed protection.
Claims (7)
1. a kind of pesticide of resisting tobacco mosaic virus, it is characterised in that：It is prepared from by the raw material of following weight portion：Radix Sophorae Flavescentiss 10 ~
30 parts, 10 ~ 30 parts of Semen Aesculi, 10 ~ 30 parts of Radix Artemisia ordosicae, 5 ~ 20 parts of Exocarpium Litchi, 5 ~ 20 parts of Fericarpium Citri Limoniae, 5 ~ 20 parts of Cortex Luffae, Folium Cinnamomi porrecti 5
~ 15 parts, 3 ~ 12 parts of Herba Rosmarini Officinalis, 3 ~ 12 parts of Fructus Forsythiae, 3 ~ 12 parts of Rhizoma Acori Graminei, 1 ~ 5 part of bacteria agent, the bacteria agent is by quality percentage
1 ~ 10% bacillus thuringiensiss of ratio, 30 ~ 40% streptomyces microflavus and 50 ~ 60% photosynthetic bacterias composition.
2. the pesticide of resisting tobacco mosaic virus as claimed in claim 1, it is characterised in that：The bacteria agent is by quality percentage
8% bacillus thuringiensiss of ratio, 35% streptomyces microflavus and 57% photosynthetic bacteria composition.
3. the pesticide of resisting tobacco mosaic virus as claimed in claim 2, it is characterised in that：Prepared by the raw material of following weight portion
Form：20 ~ 25 parts of Radix Sophorae Flavescentiss, 20 ~ 25 parts of Semen Aesculi, 15 ~ 20 parts of Radix Artemisia ordosicae, 8 ~ 12 parts of Exocarpium Litchi, 8 ~ 12 parts of Fericarpium Citri Limoniae, Cortex Luffae 8 ~
12 parts, 8 ~ 12 parts of Folium Cinnamomi porrecti, 9 parts of Herba Rosmarini Officinalis, 9 parts of Fructus Forsythiae, 9 parts of Rhizoma Acori Graminei, 2 ~ 3 parts of bacteria agent.
4. the pesticide of resisting tobacco mosaic virus as claimed in claim 3, it is characterised in that：Prepared by the raw material of following weight portion
Form：20 parts of Radix Sophorae Flavescentiss, 23 parts of Semen Aesculi, 18 parts of Radix Artemisia ordosicae, 10 parts of Exocarpium Litchi, 10 parts of Fericarpium Citri Limoniae, 10 parts of Cortex Luffae, Folium Cinnamomi porrecti 10
Part, 9 parts of Herba Rosmarini Officinalis, 9 parts of Fructus Forsythiae, 9 parts of Rhizoma Acori Graminei, 2 parts of bacteria agent.
5. a kind of preparation method of resisting tobacco mosaic virus pesticide, it is characterised in that：Comprise the following steps：
1）10 ~ 30 parts of the Radix Sophorae Flavescentiss of dry weight portion, 10 ~ 30 parts of Semen Aesculi, 10 ~ 30 parts of Radix Artemisia ordosicae, Exocarpium Litchi 5 ~ 20 is weighed respectively
3 ~ 12 parts of part, 5 ~ 20 parts of Cortex Luffae, 3 ~ 12 parts of Herba Rosmarini Officinalis, 3 ~ 12 parts of Fructus Forsythiae and Rhizoma Acori Graminei, mixing enter after freezing under nitrogen protection
Row is crushed, and obtains comminuting matter I；5 ~ 15 parts of 5 ~ 20 parts of the Fericarpium Citri Limoniae and Folium Cinnamomi porrecti of dry weight portion, mixing, powder is weighed respectively
It is broken, obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and medicinal residues I, filtrate is cooled down, sealing preserve；The water of 2 ~ 3 times of its weight is added in the medicinal residues I, is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filtered while hot obtains filtrate II and medicinal residues II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 extractum, in the extractum adds volume fraction
For 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, kept for 18 ~ 24 hours, then filter, obtain filtrate III and precipitate, by institute
State the mastic that relative density when filtrate III is evaporated to 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, in adding distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and extracting solution is closed by distillation extraction 2 ~ 3 times
And extracted with organic solvent, sub-cloud solution, vacuum revolving instrument steams organic solvent, obtains final product extract；
4）The mastic and extract are separately dried, are crushed, merged, obtain effective ingredient I；
5）The effective ingredient I is mixed with 1 ~ 5 weight portion of bacteria agent, 2000 ~ 3000rpm is stirred 5 ~ 10 minutes, is obtained final product.
6. the preparation method of resisting tobacco mosaic virus pesticide as claimed in claim 5, it is characterised in that：The extractum with it is described
The w/v 1g of ethanol：10~20ml.
7. the preparation method of resisting tobacco mosaic virus pesticide as claimed in claim 5, it is characterised in that：The organic solvent is
Dichloromethane.
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Application publication date: 20170322 |