CN106490035A - One grows tobacco special Anti-bacterium agricultural chemicals and preparation method thereof - Google Patents
One grows tobacco special Anti-bacterium agricultural chemicals and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The invention provides one grows tobacco special Anti-bacterium agricultural chemicals, belong to technical field of pesticide.One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：5 ~ 30 parts of 0.2 ~ 5 part of pyrolkigneous liquid, 0.5 ~ 5 part of chitin, 0.5 ~ 5 part of allicin and Chinese medicine composition, the Chinese medicine composition is made up of the raw material of following weight parts：10 ~ 30 parts of buckeye, 10 ~ 30 parts of argy wormwood, 5 ~ 20 parts of betel nut, 5 ~ 20 parts of oenanthe stolonifera, 5 ~ 20 parts of balloonflower root, 5 ~ 15 parts of cinnamomum camphora, blue real 3 ~ 12 parts, 3 ~ 12 parts of the capsule of weeping forsythia, 3 ~ 12 parts of oleander.Science mixture of the present invention by pyrolkigneous liquid, chitin, allicin and Chinese medicine composition, Synergistic, gained composite pesticide antibacterial ability are significantly increased, reduction tobacco leaf disease incidence, efficacy stability, safety and environmental protection, not only efficient but also saving of work and time.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to one grows tobacco special Anti-bacterium agricultural chemicals and preparation method thereof.
Background technology
Tobacco drafts a document eggplant wood, and Solanaceae is annual or limited herbaceos perennial, base portion slightly lignifying.Inflorescence basidixed, circle
Taper, spends more；Capsule ovate or square round shape, length are approximately equal to persistent calyx.Summer and autumn yields positive results.It is distributed mainly on South America, south
Sub-, Chinese.Tobacco decapacitation is made cigarette, tobacco, pipe tobacco, cigar etc. and is sucked outward for people, still has multiple medical applications.Although cigarette
Grass gives people a lot of harm of class band, or even is referred to as " poisonous weeds ", the restricted circulation of many country plaintexts or suction, and the world defends
Raw organizational member also endorsed《The Framework Convention on Tobacco Control》.But as a kind of time-honored medicinal plant, its medical value
Can not be obliterated because of its harmfulness.《National Chinese herbal medicine compilation》Record, tobacco is warm in nature sweet, poisonous, with detumescence, removing toxic substances,
The effects such as desinsection, it is mainly used in the diseases such as furuncle swelling toxin, favus of the scalp, tinea alba, the favus of the scalp, venomous snake bite, can also treats a subcutaneous ulcer, carbuncle on the back, wind
Phlegm, crane knee（Including bone tuberculosis, chronic purulent gonitis etc.）Deng disease.
Tobacco is easily endangered by multiple pest and disease damages during planting, and the species survey of pest and disease damage is more, distribution is wide, propagation
Fast and damage losses are serious.China's tobacco diseases there are about more than 60 kinds, wherein occur universal or heavier have kind more than 10.These disease pests
Evil causes very big economic loss to tobacco leaf production.Therefore, preventing and treating tobacco pest and disease damage is the important of guarantee tobacco good quality and high output
Measure.
Be presently used for tobacco chemical insecticide topmost have three kinds, including pyrethroid, organophosphorus insecticide and
Carbamate pesticide, the application of these chemical insecticides, it is ensured that the significantly volume increase of agricultural livestock products, but also give people class
It is unfavorable that the health of the environment of existence, the ecological balance and mankind itself is brought, such as soil, water body, the pollution of air and agricultural livestock products
The residual hazard of product, causes substantial amounts of person poultry poisoning and death, chemical insecticide also to injure insect while insect is killed every year
Natural enemy, destroy the ecological balance, some insects is increased sharply and ferocious is sticked up, it is difficult to preventing and treating and form vicious circle, Reusability chemistry
Insecticide, makes insect develop immunity to drugs rapidly, drug effect is declined or is failed.The update of chemical insecticide simultaneously, develops generation
Valency more and more higher, very risky.Especially to the strict demand of environmental protection and going deep into for toxicity research, to new pest control with insecticide
The restriction of agent and requirement are more and more tighter.Under these circumstances, force people to look for the new way of insect prevention, the research of insecticide and
Exploitation is not likely to produce the resistance to the action of a drug along efficient, low toxicity, insect, and the nuisanceless direction of protection pest natural enemy is developed.
Natural products comes from optimum organization of the nature through available for countless years, may more adapt to the requirement of agricultural sustainable development,
People transfer to find new biological source insecticide from nature.Biological source insecticide can by sources be divided into the metabolite of microorganism
And its derivative Anti-bacterium agricultural chemicals and Secondary Metabolite Production in Plants agrochemical.The patent of Application No. 97104754 discloses one
Grow tobacco plant protection insecticide, and it is made up of following component（Content is effective dose percentage）：Tripterygium hypoglaucum hutcs alkali 50 ~ 60%, trifoliate jewelvine
Ketone 8 ~ 15%, stemonine 8 ~ 15%, matrine 8 ~ 15%, phasins 4 ~ 8%, Strychnos nux-vomica alkali 4 ~ 8%, yunnan craibiodendron leaf element 4 ~ 8%, plant cyanogen hydrogen
Acid 4 ~ 8%.To crop nosomycosis, bacteriosis has inhibitory action, and can stimulate plant growth for the invention, strengthens degeneration-resistant border ability,
To crops common insect pests such as tobacco, wheat, rape, water fruits and vegetables, such as aphid, red spider, cabbage caterpillar, black cutworm etc. has
Tag, stomach poison function.The patent of Application No. 201610169191 discloses a kind of efficient tobacco biological medicine fertilizer, its mix proportion scheme
As follows：10% ~ 30% bacillus licheniformis；20% ~ 40% Xanthohumate；10% ~ 30% bacillus subtilis；10%~20%DA-6.This
Bright biological medicine fertilizer improve tobacco plants to fertilizer while absorbing, accomplish safe pesticide-germicide, meet cigarette
Careless agricultural production and the needs of development.Above-mentioned technology employs the biological source insecticide of low toxicity, environmental protection to some extent, shows to give birth to
Thing insecticide can prevent and treat the different pest and disease damages of tobacco, but prevention effect stability and insecticidal effect need further to carry
High.
Content of the invention
In view of this, the technical problem to be solved is to provide a kind of composite pesticide for being exclusively used in tobacco, antibacterial
Effect stability, strong drug action and environmentally safe.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is：
One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：0.5 ~ 3 part of pyrolkigneous liquid, chitin 2 ~ 6
10 ~ 30 parts of part, 0.5 ~ 5 part of allicin and Chinese medicine composition, the Chinese medicine composition is made up of the raw material of following weight parts：Sieve Suo
10 ~ 30 parts of son, 10 ~ 30 parts of argy wormwood, 5 ~ 20 parts of betel nut, 5 ~ 20 parts of oenanthe stolonifera, 5 ~ 20 parts of balloonflower root, 5 ~ 15 parts of cinnamomum camphora, indigo plant real 3 ~ 12
Part, 3 ~ 12 parts of the capsule of weeping forsythia, 3 ~ 12 parts of oleander.
Preferably, the Chinese medicine composition is made up of the raw material of following weight parts：20 ~ 25 parts of buckeye, argy wormwood 15 ~ 20
Part, 8 ~ 12 parts of betel nut, 8 ~ 12 parts of oenanthe stolonifera, 8 ~ 12 parts of balloonflower root, 8 ~ 12 parts of cinnamomum camphora, blue real 9 parts, 9 parts of the capsule of weeping forsythia, 9 parts of oleander.
Preferably, the Chinese medicine composition is made up of the raw material of following weight parts：23 parts of buckeye, 18 parts of argy wormwood, betel nut
10 parts, 10 parts of oenanthe stolonifera, 10 parts of balloonflower root, 10 parts of cinnamomum camphora, blue real 9 parts, 9 parts of the capsule of weeping forsythia, 9 parts of oleander.
Preferably, the special Anti-bacterium agricultural chemicals of the tobacco, is prepared from by the raw material of following weight portion：1 ~ 2 part of pyrolkigneous liquid,
20 ~ 25 parts of 3 ~ 4 parts of chitin, 1 ~ 3 part of allicin and Chinese medicine composition.
One preparation method for growing tobacco special Anti-bacterium agricultural chemicals, comprises the following steps：
1）Weigh 0.5 ~ 5 part of 0.5 ~ 3 part of the pyrolkigneous liquid of weight portion, 2 ~ 6 parts of chitin and allicin respectively, then temperature 30 ~
40 DEG C, carry out mixing 10 ~ 15 minutes under conditions of 2000 ~ 2500rpm of rotating speed, stand 15 ~ 20 minutes, obtain mixture；
2）10 ~ 30 parts of Chinese medicine composition for weighing weight portion is added in the mixture, 3000 ~ 3200rpm of rotating speed stirrings 30 ~ 40
Minute, stand, obtain final product.
Preferably, the Chinese medicine composition is obtained by following preparation methods, and the preparation method is comprised the following steps：
1）Buckeye, argy wormwood, betel nut, balloonflower root, Lan Shi, the capsule of weeping forsythia and the oleander of dry above-mentioned weight are weighed respectively,
Mixing, is crushed after freezing under nitrogen protection, obtains comminuting matter I；Dry above-mentioned weight is weighed respectively
Oenanthe stolonifera and cinnamomum camphora, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 ~ 3 times of its weight, it is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 medicinal extract, adds ethanol molten in the medicinal extract
Liquid, 25 ~ 30 DEG C of temperature are kept for 18 ~ 24 hours, then filter, obtain filtrate III and sediment, by III reduced pressure concentration of the filtrate
To the lotion that relative density when 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with dichloromethane, sub-cloud solution, vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
Preferably, the volume fraction of the ethanol solution is 75 ~ 80%.
Preferably, the w/v 1g of the medicinal extract and the ethanol：10~20ml.
Compared with prior art, its advantage is as follows for the present invention：
1）The invention provides one grows tobacco special Anti-bacterium agricultural chemicals, applicant has deeply research to tobacco planting, is following tobacco
On the basis of growth rhythm multiple natural constituents are carried out with mixture and research, pyrolkigneous liquid, chitin, allicin and Chinese medicine is found
Composition plays a part of Synergistic through appropriate hybrid energy so that the composite pesticide antibacterial ability for obtaining is significantly increased, and expands
Big prevention and treatment range, mitigates the resistance to the action of a drug, reduces poisoning incidence, not only efficient but also saving of work and time.
2）Composite pesticide of the present invention can be used for the multiple pest and disease damages for preventing and treating the appearance of tobacco growing cycle, mainly include tobacco
Leaf disease, wildfire, anthracnose of tobacco and frog-eye leaf spot of tobacco, field test find that the incidence of disease of tobacco mosaic disease have dropped
52.1%, disease-resistant rate is 92.7 ~ 99.5%；The incidence of disease of wildfire have dropped 71.6%, and disease-resistant rate is 93.5 ~ 99.2%；Cigarette
The incidence of disease of careless anthracnose have dropped 76.2%, and disease-resistant rate is 96.4 ~ 100%；The incidence of disease of frog-eye leaf spot of tobacco have dropped 61.8%,
Disease-resistant rate is 95.1 ~ 100%.Therefore, apply composite pesticide of the present invention significantly improve the disease-resisting, insecticidal ability of tobacco, simultaneous control
Effect is projected, and efficacy stability is with a wide range of applications.
3）The present invention is raw materials used to be low toxicity, environmental protection raw material.Research shows that pyrolkigneous liquid has bacteriostatic activity, preferably beautiful
Rice core pyrolkigneous liquid；Chitin is a kind of macromolecule polysaccharide of line style, and with antibacterial activity, preferred molecular weight is 1500 ~ 3000
Chitin, the antibacterial ability after compounding are higher；Allicin is three thio-allyl ether class compounds, is naturally occurring in liliaceous plant
In the bulb of garlic；Chinese medicine composition is formed by plurality of raw materials medicine mixing compounding, nontoxic, can not only antibacterial, and be applied to
There is tobacco promotion to increase, and improve quality of tobacco and other effects.After above-mentioned raw materials are by rationally compounding, mutual help, to tobacco
There is the pest and disease damage that whole growth cycle occurs obvious prevention effect, good absorbing effect not to have any wound to non-target object
Evil.
Specific embodiment
For a better understanding of the present invention, present disclosure, but this are further fairly set out with reference to embodiment
Bright protection content is not limited solely to the following examples.In the following description, give a large amount of concrete details so as to
More thorough understanding of the invention is provided.It will be apparent, however, to one skilled in the art that the present invention can be with
It is carried out without the need for one or more of these details.
Embodiment 1：One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：Pyrolkigneous liquid 1.5
Part, 3.5 parts of chitin, 2 parts of allicin and 25 parts of Chinese medicine composition, raw material system of the Chinese medicine composition by following weight parts
Into：23 parts of buckeye, 18 parts of argy wormwood, 10 parts of betel nut, 10 parts of oenanthe stolonifera, 10 parts of balloonflower root, 10 parts of cinnamomum camphora, blue real 9 parts, 9 parts of the capsule of weeping forsythia,
9 parts of oleander.
In the present invention, dry mature seed of the buckeye for Aesculus, surface brown or sepia, many shrinkages, concavo-convex not
Put down, somewhat gloss.Argy wormwood, is perennial herb or slightly into suffruticose plant, and plant has enhanced aroma, modern experimental research card
Bright, tarragon has antibacterial and antivirus action；Relieving asthma, antibechic and phlegm-dispelling functions；Hemostasis and blood coagulation resisting function；Calm and antiallergy
Effect；Protect liver choleretic effect etc..Betel nut, seed of the betel nut for babassu betel nut, betel nut have expelling parasite, the product that disappears, lower gas, OK
Water, effect of preventing malaria.Oenanthe stolonifera, is herb that samphire spends Chinese celery less.Balloonflower root, is the drying of Campanulaceae balloonflower root
Root.Cinnamomum camphora, is the root or stem of canella Huang camphor tree, and, containing volatile oil 2 ~ 4%, in oil, main component is safrole, contains for trunk and tree root
Amount, up to 60 ~ 95%, is secondly nopinene, phellandrene, Eugenol and cinnaldehydrum etc..Lan Shi, the fruit of blue actually polygonaceae plant indigo plant
Real.The capsule of weeping forsythia, also known as chrysanthemum bar, even shell, green grass or young crops stick up, fall to sticking up, yellow strange pellet etc., containing Neolignans, flavone compound, benzene second
Vinyl derivative etc., the pharmacological action such as have antibacterial, cardiac stimulant, diuresis, town to tell.Oleander, is the leaf of apocynaceae plant oleander
Or bark, it is used as medicine with leaf.
The preparation method of the special Anti-bacterium agricultural chemicals of the present embodiment tobacco, comprises the following steps：
1）Pyrolkigneous liquid, chitin and the allicin of above-mentioned weight portion are weighed respectively, then in 35 DEG C of temperature, rotating speed 2500rpm's
Under the conditions of carry out mixing 15 minutes, stand 20 minutes, obtain mixture；
2）The Chinese medicine composition for weighing above-mentioned weight portion is added in the mixture, and rotating speed 3000rpm is stirred 35 minutes, is stood,
Obtain final product.
Chinese medicine composition described in the present embodiment is obtained by following preparation methods：
1）Weigh dry above-mentioned buckeye, argy wormwood, betel nut, balloonflower root, Lan Shi, the capsule of weeping forsythia and oleander respectively, mix, after freezing
Crushed under nitrogen protection, obtained comminuting matter I；Weigh dry above-mentioned oenanthe stolonifera and cinnamomum camphora respectively, mix, crush, obtain
Obtain comminuting matter II；
2）The water of 5 times of its weight is added in the comminuting matter I, 50 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 times of its weight, 55 DEG C are heated to, are kept
35 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.13 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
28 DEG C, kept for 24 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.23 lotion；
3）The water of its 4 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 38 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
The medicinal extract is 1g with the w/v of the ethanol：16ml.
The using method of the special Anti-bacterium agricultural chemicals of the tobacco is：Add more than 200 times of the Anti-bacterium agricultural chemicals weight
Water, is thoroughly mixed, and makes spraying agent；Every sprinkling in 15 ~ 20 days once, prevent and treat 2 ~ 3 times.
Embodiment 2：One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：1 part of pyrolkigneous liquid,
20 parts of 4 parts of chitin, 1 part of allicin and Chinese medicine composition, the Chinese medicine composition is made up of the raw material of following weight parts：Sieve Suo
25 parts of son, 15 parts of argy wormwood, 12 parts of betel nut, 8 parts of oenanthe stolonifera, 12 parts of balloonflower root, 12 parts of cinnamomum camphora, blue real 9 parts, 9 parts of the capsule of weeping forsythia, oleander 9
Part.
The preparation method of the special Anti-bacterium agricultural chemicals of the present embodiment tobacco, comprises the following steps：
1）Pyrolkigneous liquid, chitin and the allicin of above-mentioned weight portion are weighed respectively, then in 30 DEG C of temperature, rotating speed 2000rpm's
Under the conditions of carry out mixing 15 minutes, stand 15 minutes, obtain mixture；
2）The Chinese medicine composition for weighing above-mentioned weight portion is added in the mixture, and rotating speed 3200rpm is stirred 30 minutes, is stood,
Obtain final product.
Chinese medicine composition described in the present embodiment is obtained by following preparation methods：
1）Buckeye, argy wormwood, betel nut, balloonflower root, Lan Shi, the capsule of weeping forsythia and the oleander of dry above-mentioned weight are weighed respectively,
Mixing, is crushed after freezing under nitrogen protection, obtains comminuting matter I；Dry above-mentioned weight is weighed respectively
Oenanthe stolonifera and cinnamomum camphora, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 times of its weight is added in the comminuting matter I, 40 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 times of its weight, 50 DEG C are heated to, are kept
38 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.15 medicinal extract, adds the ethanol solution that volume fraction is 80%, temperature in the medicinal extract
25 DEG C, kept for 24 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.25 lotion；
3）The water of its 3 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 35 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
The medicinal extract is 1g with the w/v of the ethanol：12ml.
The using method of the special Anti-bacterium agricultural chemicals of the tobacco is repeated no more with embodiment 1.
Embodiment 3：One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：2 parts of pyrolkigneous liquid,
23 parts of 3 parts of chitin, 3 parts of allicin and Chinese medicine composition, the Chinese medicine composition is made up of the raw material of following weight parts：Sieve Suo
20 parts of son, 20 parts of argy wormwood, 8 parts of betel nut, 12 parts of oenanthe stolonifera, 8 parts of balloonflower root, 8 parts of cinnamomum camphora, blue real 9 parts, 9 parts of the capsule of weeping forsythia, 9 parts of oleander.
The preparation method of the special Anti-bacterium agricultural chemicals of the present embodiment tobacco, comprises the following steps：
1）Pyrolkigneous liquid, chitin and the allicin of above-mentioned weight portion are weighed respectively, then in 30 ~ 40 DEG C of temperature, rotating speed 2200rpm
Under conditions of carry out mixing 10 minutes, stand 20 minutes, obtain mixture；
2）The Chinese medicine composition for weighing above-mentioned weight portion is added in the mixture, and rotating speed 3000rpm is stirred 40 minutes, is stood,
Obtain final product.
Chinese medicine composition described in the present embodiment is obtained by following preparation methods：
1）Buckeye, argy wormwood, betel nut, balloonflower root, Lan Shi, the capsule of weeping forsythia and the oleander of dry above-mentioned weight are weighed respectively,
Mixing, is crushed after freezing under nitrogen protection, obtains comminuting matter I；Dry above-mentioned weight is weighed respectively
Oenanthe stolonifera and cinnamomum camphora, mixing are crushed, and obtain comminuting matter II；
2）The water of 6 times of its weight is added in the comminuting matter I, 50 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 3 times of its weight, 60 DEG C are heated to, are kept
40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.12 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
30 DEG C, kept for 20 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.21 lotion；
3）The water of its 5 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 40 minutes, and distillation is extracted 3 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
The medicinal extract is 1g with the w/v of the ethanol：18ml.
The using method of the special Anti-bacterium agricultural chemicals of the tobacco is repeated no more with embodiment 1.
Embodiment 4：One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：Pyrolkigneous liquid 0.5
Part, 6 parts of chitin, 0.5 part of allicin and 10 parts of Chinese medicine composition, raw material system of the Chinese medicine composition by following weight parts
Into：10 parts of buckeye, 30 parts of argy wormwood, 5 parts of betel nut, 5 parts of oenanthe stolonifera, 5 parts of balloonflower root, 15 parts of cinnamomum camphora, blue real 12 parts, 12 parts of the capsule of weeping forsythia,
3 parts of oleander.
The preparation method and using method of Anti-bacterium agricultural chemicals in the present embodiment, the preparation method of Chinese medicine composition is refering to enforcement
Example 1, repeats no more.
Embodiment 5：One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：3 parts of pyrolkigneous liquid,
30 parts of 2 parts of chitin, 5 parts of allicin and Chinese medicine composition, the Chinese medicine composition is made up of the raw material of following weight parts：Sieve Suo
30 parts of son, 10 parts of argy wormwood, 20 parts of betel nut, 20 parts of oenanthe stolonifera, 20 parts of balloonflower root, 5 parts of cinnamomum camphora, blue real 3 parts, 3 parts of the capsule of weeping forsythia, oleander 12
Part.
The preparation method and using method of Anti-bacterium agricultural chemicals in the present embodiment, the preparation method of Chinese medicine composition is refering to enforcement
Example 1, repeats no more.
Embodiment 6：One grows tobacco special Anti-bacterium agricultural chemicals, is prepared from by the raw material of following weight portion：Pyrolkigneous liquid 0.8
Part, 5 parts of chitin, 4 parts of allicin and 15 parts of Chinese medicine composition, the Chinese medicine composition is made up of the raw material of following weight parts：
15 parts of buckeye, 25 parts of argy wormwood, 15 parts of betel nut, 15 parts of oenanthe stolonifera, 15 parts of balloonflower root, 6 parts of cinnamomum camphora, blue real 6 parts, 6 parts of the capsule of weeping forsythia, folder bamboo
10 parts of peach.
The preparation method and using method of Anti-bacterium agricultural chemicals in the present embodiment, the preparation method of Chinese medicine composition is refering to enforcement
Example 1, repeats no more.
Embodiment 7：The special Anti-bacterium agricultural chemicals of tobacco described by the present embodiment, as different from Example 1：
The special Anti-bacterium agricultural chemicals of the tobacco, also includes：1.5 weight portion of microbial bacterial agent, the microbial bacterial agent is by quality point
Several 35% bacillus subtilises and 65% streptomyces microflavus of mass fraction composition.
Bacillus subtilis, Bacillus subtilis, living bacteria count >=100,000,000,000 cfu/g；Streptomyces microflavus,
Streptomyces microflavus, living bacteria count >=1.5 hundred million/g.
The special Anti-bacterium agricultural chemicals of the present embodiment tobacco is as different from Example 1：Step 2）Chinese medicine composition is weighed respectively
Add in the mixture with microbial bacterial agent, rotating speed 3000rpm is stirred 40 minutes, stand, obtain final product.
Embodiment 8：The special Anti-bacterium agricultural chemicals of tobacco described by the present embodiment, with embodiment 1 except that：
The special Anti-bacterium agricultural chemicals of the tobacco, also includes：2.0 weight portion of microbial bacterial agent, the microbial bacterial agent is by quality point
Several 45% bacillus subtilises and 55% streptomyces microflavus of mass fraction composition.
The special Anti-bacterium agricultural chemicals of the present embodiment tobacco is as different from Example 1：Step 2）Chinese medicine composition is weighed respectively
Add in the mixture with microbial bacterial agent, rotating speed 3000rpm is stirred 35 minutes, stand, obtain final product.
Comparative example 1：The special Anti-bacterium agricultural chemicals of tobacco, is prepared from by the raw material of following weight portion：1.5 parts of pyrolkigneous liquid, first
Shell element 3.5 parts, 2 parts of allicin.
Comparative example 2：The special Anti-bacterium agricultural chemicals of tobacco, is prepared from by the raw material of following weight portion：23 parts of buckeye, argy wormwood
18 parts, 10 parts of betel nut, 10 parts of oenanthe stolonifera, 10 parts of balloonflower root, 10 parts of cinnamomum camphora, blue real 9 parts, 9 parts of the capsule of weeping forsythia, 9 parts of oleander.
Effect assessment
Antibacterial tests
1.1 reagent agent
Experimental group：ⅠA：Anti-bacterium agricultural chemicals prepared by the embodiment of the present invention 1；
ⅠB：Anti-bacterium agricultural chemicals prepared by the embodiment of the present invention 7；
Control group：ⅡA：Anti-bacterium agricultural chemicals prepared by comparative example 1；
ⅡB：Anti-bacterium agricultural chemicals prepared by comparative example 2.
1.2 inhibitory activity are tested
1）Assay method 1
For trying germ：Tobacco mosaic virus (TMV)（Tobacco mosaic virus）.
Test plant：Withered spot host's Nicotiana glutinosa.By Nicotiana glutinosa seed first with the 30min that hots water treatment of seeds during nursery.Then will plant
Son is directly sowed in seedling-growing container, puts it into illumination box, and 22 DEG C of temperature, illumination 16h/d, humidity 60% are cultivated, when
Tobacco supplies examination when growing to 5 ~ 6 true leaves.
Inoculation：It is inoculated with using juice rubbing manipulation；
From 5 ~ 6 leaf phase Nicotiana glutinosas as the withered spot host for supplying examination germ；
Test is carried out in insect prevention greenhouse.Reagent agent is taken, the 0.05g/ml aqueous solution is configured to distilled water, add for examination disease
Bacterium, makes the final concentration of 10 μ g/ml of virus.Mixing 10min, left using half leaf method frictional inoculation in withered spot host Nicotiana glutinosa（Right）Half
Ye Shang, separately with the distilled water containing TMV viruses（The final concentration of 10 μ g/ml of virus）For control, 5 leaves of inoculation, test weight is often processed
Multiple 3 times.After 3 days after obvious withered spot to appear, record withered spot number, calculates inhibiting rate in time（%）, commented with average inhibition
Valency inhibition；
Inhibiting rate=（Control withered spot number-process withered spot number）/ control withered spot number × 100.
2）Assay method 2
For trying germ：Anthrax-bacilus（Colletotrichum destructivum O'Gara）, tobacco tail spore bacterium（Cercospora
nicotianae Ell. et Ev.）, prairie fire germ（Pseudomonas tabaci (Wolf et Foster)
Stevens）.
Reagent agent adopts sessile drop method to pathogen conidia germination inhibitory action, adds 0.1% mercuric chloride and suppress before counting
Its rudiment, the different visuals field count more than 100 spores, the little micro dmso solution of the crude extract for being not readily dissolved in water of polarity
After be diluted with water to desired concn and tested, also add same amount dimethyl sulfoxide (DMSO) in the control group aqueous solution.
The inhibitory action of pathogen mycelial growth adopts growth rate method.Under aseptic condition, with the punching that aperture is 0.8cm
Device takes the eugonic bacterial classification of colony edge and moves on the PDA culture plates containing extract 0.01g/mL that (extract is being processed
Before first pass through high-temperature sterilization, then allocate concentration with sterilized water, to reduce pollution.After the little crude extract of polarity is first dissolved in acetone
Mix with 40 ~ 50 DEG C of agar mediums again, be uniformly dispersed with profit), with culture medium, add equivalent sterilized water to process weight as control, often
Multiple 3 times, 26 DEG C incubated, when control bacterium colony is close to culture dish edge, determines colony diameter, and each bacterium colony presses right-angled intersection
Method is measured 2 times, with the size that its average represents bacterium colony, calculates inhibiting rate.
Inhibiting rate computing formula is as follows：
Inhibition of germination (%)=(control spore germination number-process spore germination number)/control spore germination number × 100；
Mycelial growth inhibition rate (%)=(the pure increment of the pure increment-process of control)/control colony diameter × 100.
1.3 reagent agents are to supplying the bacteriostatic activity for trying germ
1 reagent agent of table is to supplying the inhibiting rate for trying germ
As it can be seen from table 1 the Anti-bacterium agricultural chemicals prepared by the embodiment of the present invention 1 to the inhibiting rate of tobacco mosaic virus (TMV) is
98.7%, obviously higher than comparative example 1 and 2, simultaneously above the inhibiting rate sum of comparative example 1 and 2, reach the mesh of Synergistic
's.Anti-bacterium agricultural chemicals of the present invention to the inhibition of germination of prairie fire germ, anthrax-bacilus and tobacco tail spore bacterium more than 80%,
Inhibition of germination highest of the Anti-bacterium agricultural chemicals that wherein prepared by embodiment to anthrax-bacilus, is 95.6%, prepared by embodiment 7
Anti-bacterium agricultural chemicals, to the inhibition of germination highest to anthrax-bacilus, is 96.2%；And comparative example 1 and 2 pairs of prairie fire germs, anthraxs
The inhibition of germination fluctuation of bacterium and tobacco tail spore bacterium is larger, for example：Comparative example 1 to the inhibition of germination of anthrax-bacilus is
31.6%, comparative example 2 is 47.2% to the inhibition of germination of tobacco tail spore bacterium.Anti-bacterium agricultural chemicals of the present invention to prairie fire germ,
, more than 75%, the Anti-bacterium agricultural chemicals that wherein prepared by embodiment is to open country for the mycelial growth inhibition rate of anthrax-bacilus and tobacco tail spore bacterium
The mycelial growth inhibition rate highest of fiery germ, is 97.5%；Comparatively speaking, the Mycelial growth of 1 and 2 pairs of three kinds of pathogens of comparative example
Rate is substantially weaker.
Additionally, group I A and I B are Comparatively speaking, every effect of group I B is better than I A, inhibition is had and is increased
Use is pretended, can be used as more preferred scheme.Above-mentioned experiment shows that Anti-bacterium agricultural chemicals of the present invention is used for the preventing and treating of tobacco leaf germ
There is notable and stable inhibition, strong drug action, synergy are obvious.
Although the foregoing describing the specific embodiment of the present invention, those familiar with the art should manage
Solution, the specific embodiment described by us are merely exemplary, rather than for the restriction to the scope of the present invention, are familiar with this
Equivalent modification and change that the technical staff in field is made in the spirit according to the present invention, should all cover the present invention's
In scope of the claimed protection.
Claims (8)
1. one grow tobacco special Anti-bacterium agricultural chemicals, it is characterised in that：It is prepared from by the raw material of following weight portion：Pyrolkigneous liquid 0.5 ~
10 ~ 30 parts of 3 parts, 2 ~ 6 parts of chitin, 0.5 ~ 5 part of allicin and Chinese medicine composition, the Chinese medicine composition is by following weight parts
Raw material is made：10 ~ 30 parts of buckeye, 10 ~ 30 parts of argy wormwood, 5 ~ 20 parts of betel nut, 5 ~ 20 parts of oenanthe stolonifera, 5 ~ 20 parts of balloonflower root, cinnamomum camphora 5 ~
15 parts, blue real 3 ~ 12 parts, 3 ~ 12 parts of the capsule of weeping forsythia, 3 ~ 12 parts of oleander.
2. the special Anti-bacterium agricultural chemicals of tobacco as claimed in claim 1, it is characterised in that：The Chinese medicine composition is by following weight
The raw material of part is made：20 ~ 25 parts of buckeye, 15 ~ 20 parts of argy wormwood, 8 ~ 12 parts of betel nut, 8 ~ 12 parts of oenanthe stolonifera, 8 ~ 12 parts of balloonflower root, perfume
8 ~ 12 parts of camphor tree, blue real 9 parts, 9 parts of the capsule of weeping forsythia, 9 parts of oleander.
3. the special Anti-bacterium agricultural chemicals of tobacco as claimed in claim 2, it is characterised in that：The Chinese medicine composition is by following weight
The raw material of part is made：23 parts of buckeye, 18 parts of argy wormwood, 10 parts of betel nut, 10 parts of oenanthe stolonifera, 10 parts of balloonflower root, 10 parts of cinnamomum camphora, indigo plant real 9
Part, 9 parts of the capsule of weeping forsythia, 9 parts of oleander.
4. the special Anti-bacterium agricultural chemicals of tobacco as claimed in claim 3, it is characterised in that：The special Anti-bacterium agricultural chemicals of the tobacco,
It is prepared from by the raw material of following weight portion：1 ~ 2 part of pyrolkigneous liquid, 3 ~ 4 parts of chitin, 1 ~ 3 part of allicin and Chinese medicine composition 20 ~
25 parts.
5. the special Anti-bacterium agricultural chemicals of tobacco as described in any one of claim 1 ~ 4, it is characterised in that：The Chinese medicine composition leads to
Cross following preparation methods to obtain, the preparation method is comprised the following steps：
1）Buckeye, argy wormwood, betel nut, balloonflower root, Lan Shi, the capsule of weeping forsythia and the oleander of dry above-mentioned weight are weighed respectively,
Mixing, is crushed after freezing under nitrogen protection, obtains comminuting matter I；Dry above-mentioned weight is weighed respectively
Oenanthe stolonifera and cinnamomum camphora, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 ~ 3 times of its weight, it is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 medicinal extract, adds ethanol molten in the medicinal extract
Liquid, 25 ~ 30 DEG C of temperature are kept for 18 ~ 24 hours, then filter, obtain filtrate III and sediment, by III reduced pressure concentration of the filtrate
To the lotion that relative density when 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with dichloromethane, sub-cloud solution, vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
6. the special Anti-bacterium agricultural chemicals of tobacco as claimed in claim 5, it is characterised in that：The volume fraction of the ethanol solution is
75~80%.
7. the special Anti-bacterium agricultural chemicals of tobacco as claimed in claim 6, it is characterised in that：The weight of the medicinal extract and the ethanol
Volume ratio 1g：10~20ml.
8. a preparation method for growing tobacco special Anti-bacterium agricultural chemicals, it is characterised in that：Comprise the following steps：
1）Weigh 0.5 ~ 5 part of 0.5 ~ 3 part of the pyrolkigneous liquid of weight portion, 2 ~ 6 parts of chitin and allicin respectively, then temperature 30 ~
40 DEG C, carry out mixing 10 ~ 15 minutes under conditions of 2000 ~ 2500rpm of rotating speed, stand 15 ~ 20 minutes, obtain mixture；
2）10 ~ 30 parts of Chinese medicine composition for weighing weight portion is added in the mixture, 3000 ~ 3200rpm of rotating speed stirrings 30 ~ 40
Minute, stand, obtain final product.
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| CN113349227A (en) * | 2021-05-12 | 2021-09-07 | 湖南省烟草公司株洲市公司 | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method |
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| CN116477992A (en) * | 2023-04-23 | 2023-07-25 | 山东省博兴县薪利生物质能有限公司 | Liquid fertilizer using wood vinegar as raw material and preparation method thereof |
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Application publication date: 20170315 |