CN108341716A - A kind of antibacterial growth-promoting elongated biological pesticide of plant source - Google Patents
A kind of antibacterial growth-promoting elongated biological pesticide of plant source Download PDFInfo
- Publication number
- CN108341716A CN108341716A CN201810281364.XA CN201810281364A CN108341716A CN 108341716 A CN108341716 A CN 108341716A CN 201810281364 A CN201810281364 A CN 201810281364A CN 108341716 A CN108341716 A CN 108341716A
- Authority
- CN
- China
- Prior art keywords
- plant source
- extract
- biological pesticide
- oleander
- antibacterial growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of antibacterial growth-promoting elongated biological pesticide of plant source, including plant source Substance, nitrogen salt, sylvite with, microcosmic salt, vitamin and amino acid；A concentration of 2.0~2.5g/100ml of the plant source Substance, the plant source Substance are made of Apocynaceae extract and chitin nano fiber.The plant source Substance of the present invention is Apocynaceae extract and chitin nano fiber, and active constituent content is high, and fungistatic effect is strong；Botanical pesticide ingredient is simply easily quantitative；Effect is fast, efficacy stability, and quick-acting is strong；Nitrogen salt, sylvite, microcosmic salt, vitamin and amino acid in pesticide promote the growth of biology.For the present invention to crops without key residual, free from environmental pollution and agricultural product, highly effective and safe is of low cost.
Description
Technical field
The present invention relates to field of biological pesticide, especially a kind of antibacterial growth-promoting elongated biological pesticide of plant source.
Background technology
Pesticide can not only prevent various biologies harmful to crops, improve the yield of crops, moreover it is possible to improve crops
Resistance, adjust crop growth, improve the quality of crops.Pesticide has thousands of years of usage history, such as early in Christian era
First 1500 to B.C. 1000, Egypt began to use Urginea maritima deinsectization, Greece and Rome to use sulphur desinsection etc..In recent years
Come, makes as DDT, 666 etc. synthesize organic insecticide and other organochlorines, the production of organophosphor and pyrethrin pesticide and popularization
With the chemical pesticide of organic synthesis occupies the leading position of crops pesticide.But chemical pesticide is non-naturally occurring mostly
Substance, residual life, is long and not degradable, in addition long-term unreasonable use, causes environmental pollution, destroys the drawbacks such as the ecological balance,
Also endanger human and livestock health simultaneously.As the proposition and the mankind of the strategy of sustainable development are to the increasingly heavy of own health and environmental problem
Depending on, environment compatibility, the research and development of botanical pesticide of good, safety, low-residual are increasingly subject to the attention of people.Plant source
Substance have many advantages, such as it is rapid, resourceful, at low cost and renewable without apparent phytotoxicity, environment degradable,
Have become one of the research hotspot of modern.
China has been achieved with some progress in the research of the antibacterial biological pesticide of plant source, but plant source at this stage presses down
Bacterium biological pesticide remains that active constituent content is low, and fungistatic effect is poor；Botanical pesticide complicated component, it is difficult to quantitative；Make
With the defects of slow, drug effect is unstable, and quick-acting is poor.
Invention content
In order to overcome active constituent content during the antibacterial growth promotion of botanical biological pesticide in the prior art low, antibacterial effect
Fruit is poor；Botanical pesticide complicated component, it is difficult to quantitative；The defects of effect is slow, and drug effect is unstable, and quick-acting is poor, the present invention carries
For a kind of antibacterial growth-promoting elongated biological pesticide of plant source.
The technical solution adopted by the present invention to solve the technical problems is：A kind of antibacterial growth-promoting elongated biology agriculture of plant source
Medicine, including plant source Substance, nitrogen salt, sylvite with, microcosmic salt, vitamin and amino acid, by plant source bacteriostatic activity object
Matter dissolved in purified water, a concentration of 2.0~2.5g/100ml of plant source Substance, then be added nitrogen salt, sylvite with
And microcosmic salt, it is eventually adding vitamin and amino acid；The plant source Substance is by Apocynaceae extract and chitin
Nanofiber forms, and the mass ratio of the Apocynaceae extract and chitin nano fiber is 90~100：20~30；It is described
The preparation method of Apocynaceae extract is：(1) sample collection：Take first apocynaceae plant oleander, sour leaf glue rattan with
And the fresh cauline leaf of goat's horn col, it is dry, then pulverizer is utilized to crush, sieved for subsequent use；(2) coarse extraction：It is dry to weigh step (1)
Dry oleander 100g is fitted into the double-deck tea bag set, by the closed deflation of sack after installing, is then charged into Soxhlet extraction
It in device, is heated to reflux, is extracted using 65~75% ethanol solution, filtrate concentration is heated to reflux the oleander to tea bag
Until substantially colorless；Obtain the oleander solids of ethyl alcohol extraction；(3) it extracts：The oleander extracted to step (2) ethyl alcohol is solid
Petroleum ether is added in body object, stirring utilizes petroleum ether, benzene, extracting n-butyl alcohol successively until solids dissolving；It handles repeatedly more
It is secondary, it is then concentrated under reduced pressure, respectively obtains petroleum ether extract, benzene extract and n-butyl alcohol extract；(4) it pre-processes：Xiang Bu
Suddenly 2~3 times of silica white is added in the n-butyl alcohol extract that (3) obtain, and is uniformly mixed, 50~65 DEG C of drying；(5) column is filled：Fill column
Petroleum ether elution system solvent is first poured into before, and the silica gel sample for then obtaining step (4) is put into pillar；(6) it elutes, receive
Collection：It is eluted first with petroleum ether, a small amount of petroleum ether solvent is first added in when elution, opens simultaneously pillar piston, keeps solution slow
Slug flow goes out until sample largely enters in column in silica gel, then fills it up with solvent, elutes 15~20d；Again with benzene elution 15~
20d finally elutes 15~20d with n-butanol, is concentrated under reduced pressure, and is spray-dried up to oleander extract；Sour leaf glue rattan and sheep
Angle col repeats step (2)~(6) to get sour leaf glue boisiana extract and goat's horn col extract.
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, the concentration of the nitrogen salt, sylvite and microcosmic salt is 1
~2g/100ml.
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, the nitrogen salt include ammonium hydrogen carbonate, ammonium carbonate and
The mass ratio of ammonium chloride, the ammonium hydrogen carbonate, ammonium carbonate and ammonium chloride is 1~2：0.8~1.6：0.8~1.6.
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, the sylvite include potassium chloride, potassium sulfate, nitric acid
Potassium, potassium carbonate and saleratus, the potassium chloride, potassium sulfate, potassium nitrate, potassium carbonate and saleratus mixture matter
Amount is than being 3~4：2.5~3：2~3：1.5~2.5：1~2.5.
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, the microcosmic salt include calcium phosphate, magnesium phosphate, ferric phosphate
And trbasic zinc phosphate, the calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate mass ratio be 1.5~4：1.5~4：1~3.5：1
~3.5.
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, a concentration of 0.5~0.8g/ of the vitamin
100ml, a concentration of 0.2~0.8g/100ml of the amino acid.
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, the chitin nano fiber a diameter of 200~
500nm。
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, the antibacterial growth-promoting elongated biological pesticide of plant source are answered
For Agrobacterium tumefaciens, bacillus subtilis, Escherichia coli, avenae subsp.citrull, Bacterial Wilt of Eucalyptus bacterium, tomato Streptomyces scabies
And ash arrhizus bacteria.
A kind of antibacterial growth-promoting elongated biological pesticide of above-mentioned plant source, oleander is extracted in the Apocynaceae extract
The mass ratio of object, sour leaf glue boisiana extract and goat's horn col extract is 1~5：2~3：1.5~3.
The present invention has the following advantages and high-lighting effect compared with prior art：
The invention has the advantages that the antibacterial growth-promoting elongated biological pesticide plant source Substance of plant source of the present invention
Predominantly Apocynaceae extract and chitin nano fiber can effectively eliminate the antibacterial growth-promoting of traditional plant source biological pesticide
Active constituent content is low in growth process, and fungistatic effect is poor；Botanical pesticide complicated component, it is difficult to quantitative；Effect is slow, and drug effect is not
Stablize, the defect of quick-acting difference, in addition, nitrogen salt, sylvite, microcosmic salt, vitamin in the antibacterial growth-promoting elongated biological pesticide of plant source
And amino acid can promote the growth of biology.The present invention to crops without key residual, free from environmental pollution and agricultural product, efficiently
Safety, it is of low cost.
Specific implementation mode
In order to illustrate more clearly of present disclosure, below the present invention is described further, but following implement
Example is merely to illustrate the present invention, is not limited to the scope of the present invention.For for those of ordinary skill in the art, do not paying
Under the premise of going out creative work, other embodiments are obtained according to embodiment, belong to protection scope of the present invention.
【Embodiment 1】
A kind of antibacterial growth-promoting elongated biological pesticide of plant source, including plant source Substance, nitrogen salt, sylvite are with, phosphorus
Salt, vitamin and amino acid, by plant source Substance dissolved in purified water, plant source Substance it is a concentration of
Then 2.0g/100ml is added nitrogen salt, sylvite and microcosmic salt, is eventually adding vitamin and amino acid；The antibacterial work of plant source
Property substance is made of Apocynaceae extract and chitin nano fiber, the Apocynaceae extract and chitin nano fiber
Mass ratio be 90：20；The preparation method of the Apocynaceae extract is：(1) sample collection：Apocynaceae is taken to plant first
The fresh cauline leaf of object oleander, sour leaf glue rattan and goat's horn col, it is dry, then pulverizer is utilized to crush, sieved for subsequent use；(2) thick
Extraction：The dry oleander 100g of step (1) is weighed, is fitted into the double-deck tea bag set, it is after installing that sack is closed tight
Contracting, be then charged into Soxhlet extraction device, be heated to reflux using 65% ethanol solution, extract, filtrate concentration, be heated to reflux to
Until oleander in tea bag is substantially colorless；Obtain the oleander solids of ethyl alcohol extraction；(3) it extracts：It is carried to step (2) ethyl alcohol
Petroleum ether is added in the oleander solids got, stirring utilizes petroleum ether, benzene, n-butanol to extract successively until solids dissolving
It takes；Processing is multiple repeatedly, is then concentrated under reduced pressure, respectively obtains petroleum ether extract, benzene extract and n-butyl alcohol extract；
(4) it pre-processes：2 times of silica white is added in the n-butyl alcohol extract obtained to step (3), is uniformly mixed, 50 DEG C of drying；(5) it fills
Column：Petroleum ether elution system solvent is first poured into before dress column, the silica gel sample for then obtaining step (4) is put into pillar；(6)
Elution is collected：It is eluted first with petroleum ether, a small amount of petroleum ether solvent is first added in when elution, opens simultaneously pillar piston, makes
Solution slowly then filled it up with, elute 15d until sample largely enters in column in silica gel by outflow by solvent；It is eluted again with benzene
15d finally elutes 15d with n-butanol, is concentrated under reduced pressure, and is spray-dried up to oleander extract；Sour leaf glue rattan and goat's horn col
Step (2)~(6) are repeated to get sour leaf glue boisiana extract and goat's horn col extract.
The concentration of the nitrogen salt, sylvite and microcosmic salt is 1g/100ml；The nitrogen salt include ammonium hydrogen carbonate, ammonium carbonate with
And ammonium chloride, the mass ratio of the ammonium hydrogen carbonate, ammonium carbonate and ammonium chloride is 1：0.8：0.8；The sylvite include potassium chloride,
Potassium sulfate, potassium nitrate, potassium carbonate and saleratus, the potassium chloride, potassium sulfate, potassium nitrate, potassium carbonate and saleratus
Mixture mass ratio be 3：2.5：2：1.5：1；The microcosmic salt includes calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate, institute
The mass ratio for stating calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate is 1.5：1.5：1：1；A concentration of 0.5g/ of the vitamin
100ml, a concentration of 0.2g/100ml of the amino acid；A diameter of 200nm of the chitin nano fiber；The plant source
Antibacterial growth-promoting elongated biological pesticide is applied to Agrobacterium tumefaciens, bacillus subtilis, Escherichia coli, avenae subsp.citrull, eucalyptus
Set ralstonia solanacearum, tomato Streptomyces scabies and ash arrhizus bacteria；Oleander extract, sour leaf glue rattan in the Apocynaceae extract
The mass ratio of extract and goat's horn col extract is 1：2：1.5.
【Embodiment 2】
A kind of antibacterial growth-promoting elongated biological pesticide of plant source, including plant source Substance, nitrogen salt, sylvite are with, phosphorus
Salt, vitamin and amino acid, by plant source Substance dissolved in purified water, plant source Substance it is a concentration of
Then 2.5g/100ml is added nitrogen salt, sylvite and microcosmic salt, is eventually adding vitamin and amino acid；The antibacterial work of plant source
Property substance is made of Apocynaceae extract and chitin nano fiber, the Apocynaceae extract and chitin nano fiber
Mass ratio be 100：30；The preparation method of the Apocynaceae extract is：(1) sample collection：Apocynaceae is taken first
The fresh cauline leaf of plant oleander, sour leaf glue rattan and goat's horn col, it is dry, then pulverizer is utilized to crush, sieved for subsequent use；(2)
Coarse extraction：The dry oleander 100g of step (1) is weighed, is fitted into the double-deck tea bag set, it is after installing that sack is closed
Tighten, be then charged into Soxhlet extraction device, be heated to reflux using 75% ethanol solution, extract, filtrate concentration is heated to reflux
Until the oleander in tea bag is substantially colorless；Obtain the oleander solids of ethyl alcohol extraction；(3) it extracts：To step (2) ethyl alcohol
Petroleum ether is added in the oleander solids extracted, stirring utilizes petroleum ether, benzene, n-butanol successively until solids dissolving
Extraction；Processing is multiple repeatedly, is then concentrated under reduced pressure, respectively obtains petroleum ether extract, benzene extract and n-butyl alcohol extract；
(4) it pre-processes：3 times of silica white is added in the n-butyl alcohol extract obtained to step (3), is uniformly mixed, 65 DEG C of drying；(5) it fills
Column：Petroleum ether elution system solvent is first poured into before dress column, the silica gel sample for then obtaining step (4) is put into pillar；(6)
Elution is collected：It is eluted first with petroleum ether, a small amount of petroleum ether solvent is first added in when elution, opens simultaneously pillar piston, makes
Solution slowly then filled it up with, elute 20d until sample largely enters in column in silica gel by outflow by solvent；It is eluted again with benzene
20d finally elutes 20d with n-butanol, is concentrated under reduced pressure, and is spray-dried up to oleander extract；Sour leaf glue rattan and goat's horn col
Step (2)~(6) are repeated to get sour leaf glue boisiana extract and goat's horn col extract.
The concentration of the nitrogen salt, sylvite and microcosmic salt is 2g/100ml；The nitrogen salt include ammonium hydrogen carbonate, ammonium carbonate with
And ammonium chloride, the mass ratio of the ammonium hydrogen carbonate, ammonium carbonate and ammonium chloride is 2：1.6：1.6；The sylvite include potassium chloride,
Potassium sulfate, potassium nitrate, potassium carbonate and saleratus, the potassium chloride, potassium sulfate, potassium nitrate, potassium carbonate and saleratus
Mixture mass ratio be 4：3：3：2.5：2.5；The microcosmic salt includes calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate, institute
The mass ratio for stating calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate is 4：4：3.5：3.5；A concentration of 0.8g/ of the vitamin
100ml, a concentration of 0.8g/100ml of the amino acid；A diameter of 500nm of the chitin nano fiber；The plant source
Antibacterial growth-promoting elongated biological pesticide is applied to Agrobacterium tumefaciens, bacillus subtilis, Escherichia coli, avenae subsp.citrull, eucalyptus
Set ralstonia solanacearum, tomato Streptomyces scabies and ash arrhizus bacteria；Oleander extract, sour leaf glue rattan in the Apocynaceae extract
The mass ratio of extract and goat's horn col extract is 5：3：3.
【Embodiment 3】
A kind of antibacterial growth-promoting elongated biological pesticide of plant source, including plant source Substance, nitrogen salt, sylvite are with, phosphorus
Salt, vitamin and amino acid, by plant source Substance dissolved in purified water, plant source Substance it is a concentration of
Then 2.25g/100ml is added nitrogen salt, sylvite and microcosmic salt, is eventually adding vitamin and amino acid；The antibacterial work of plant source
Property substance is made of Apocynaceae extract and chitin nano fiber, the Apocynaceae extract and chitin nano fiber
Mass ratio be 95：25；The preparation method of the Apocynaceae extract is：(1) sample collection：Apocynaceae is taken to plant first
The fresh cauline leaf of object oleander, sour leaf glue rattan and goat's horn col, it is dry, then pulverizer is utilized to crush, sieved for subsequent use；(2) thick
Extraction：The dry oleander 100g of step (1) is weighed, is fitted into the double-deck tea bag set, it is after installing that sack is closed tight
Contracting, be then charged into Soxhlet extraction device, be heated to reflux using 70% ethanol solution, extract, filtrate concentration, be heated to reflux to
Until oleander in tea bag is substantially colorless；Obtain the oleander solids of ethyl alcohol extraction；(3) it extracts：It is carried to step (2) ethyl alcohol
Petroleum ether is added in the oleander solids got, stirring utilizes petroleum ether, benzene, n-butanol to extract successively until solids dissolving
It takes；Processing is multiple repeatedly, is then concentrated under reduced pressure, respectively obtains petroleum ether extract, benzene extract and n-butyl alcohol extract；
(4) it pre-processes：2.5 times of silica white is added in the n-butyl alcohol extract obtained to step (3), is uniformly mixed, 60 DEG C of drying；(5)
Fill column：Petroleum ether elution system solvent is first poured into before dress column, the silica gel sample for then obtaining step (4) is put into pillar；
(6) it elutes, collect：It is eluted first with petroleum ether, a small amount of petroleum ether solvent is first added in when elution, opens simultaneously pillar work
Plug makes solution slowly flow out until sample largely enters in column in silica gel, then fills it up with solvent, elution 18d；It is washed again with benzene
De- 18d, finally elutes 18d with n-butanol, is concentrated under reduced pressure, and is spray-dried up to oleander extract；Sour leaf glue rattan and goat's horn
Col repeats step (2)~(6) to get sour leaf glue boisiana extract and goat's horn col extract.
The concentration of the nitrogen salt, sylvite and microcosmic salt is 1.5g/100ml；The nitrogen salt includes ammonium hydrogen carbonate, ammonium carbonate
And ammonium chloride, the mass ratio of the ammonium hydrogen carbonate, ammonium carbonate and ammonium chloride is 1.5：1：1；The sylvite include potassium chloride,
Potassium sulfate, potassium nitrate, potassium carbonate and saleratus, the potassium chloride, potassium sulfate, potassium nitrate, potassium carbonate and saleratus
Mixture mass ratio be 3.5：2.5：2.5：2：1.5；The microcosmic salt includes calcium phosphate, magnesium phosphate, ferric phosphate and phosphoric acid
Zinc, the calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate mass ratio be 2：2：1.5：2；The vitamin it is a concentration of
0.6g/100ml, a concentration of 0.5g/100ml of the amino acid；A diameter of 300nm of the chitin nano fiber；It is described
The antibacterial growth-promoting elongated biological pesticide of plant source is applied to Agrobacterium tumefaciens, bacillus subtilis, Escherichia coli, angular leaf spot of cucumber
Bacterium, Bacterial Wilt of Eucalyptus bacterium, tomato Streptomyces scabies and ash arrhizus bacteria；Oleander extract, acid in the Apocynaceae extract
The mass ratio of leaf glue boisiana extract and goat's horn col extract is 2：2.5：2.
Above example is only exemplary embodiment of the present invention, is not used in the limitation present invention, protection scope of the present invention
It is defined by the claims.Those skilled in the art can within the spirit and scope of the present invention make respectively the present invention
Kind modification or equivalent replacement, this modification or equivalent replacement also should be regarded as being within the scope of the present invention.
Claims (9)
1. a kind of antibacterial growth-promoting elongated biological pesticide of plant source, including plant source Substance, nitrogen salt, sylvite with, microcosmic salt,
Vitamin and amino acid, it is characterised in that：By plant source Substance dissolved in purified water, plant source Substance
A concentration of 2.0~2.5g/100ml, nitrogen salt, sylvite and microcosmic salt is then added, is eventually adding vitamin and amino acid；It is described
Plant source Substance is made of Apocynaceae extract and chitin nano fiber, the Apocynaceae extract and first
The mass ratio of shell element nanofiber is 90~100：20~30；The preparation method of the Apocynaceae extract is：(1) sample is adopted
Collection：The fresh cauline leaf of apocynaceae plant oleander, sour leaf glue rattan and goat's horn col is taken first, it is dry, then utilize crushing
Machine crushes, and sieves for subsequent use；(2) coarse extraction：The dry oleander 100g of step (1) is weighed, the double-deck tea bag set is packed into
In, it by the closed deflation of sack after installing, is then charged into Soxhlet extraction device, is heated back using 65~75% ethanol solution
Stream, extraction, filtrate concentration are heated to reflux until the oleander in tea bag is substantially colorless；The oleander for obtaining ethyl alcohol extraction is solid
Body object；(3) it extracts：Petroleum ether is added in the oleander solids extracted to step (2) ethyl alcohol, stirring is until solids is molten
Solution utilizes petroleum ether, benzene, extracting n-butyl alcohol successively；Processing is multiple repeatedly, is then concentrated under reduced pressure, respectively obtains petroleum ether extraction
Object, benzene extract and n-butyl alcohol extract；(4) it pre-processes：The n-butyl alcohol extract obtained to step (3) is added 2~3 times
Silica white is uniformly mixed, 50~65 DEG C of drying；(5) column is filled：Petroleum ether elution system solvent is first poured into before dress column, then will
The silica gel sample that step (4) obtains is put into pillar；(6) it elutes, collect：It is eluted first with petroleum ether, when elution is first added
A small amount of petroleum ether solvent opens simultaneously pillar piston, so that solution is slowly flowed out and enters in column in silica gel up to sample is most of,
Then solvent is filled it up with, elutes 15~20d；15~20d is eluted with benzene again, finally elutes 15~20d with n-butanol, decompression is dense
Contracting is spray-dried up to oleander extract；Sour leaf glue rattan and goat's horn col repeat step (2)~(6) and are carried to get sour leaf glue rattan
Take object and goat's horn col extract.
2. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 1, which is characterized in that the nitrogen salt,
The concentration of sylvite and microcosmic salt is 1~2g/100ml.
3. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 2, which is characterized in that the nitrogen salt packet
Ammonium hydrogen carbonate, ammonium carbonate and ammonium chloride are included, the mass ratio of the ammonium hydrogen carbonate, ammonium carbonate and ammonium chloride is 1~2：0.8~
1.6：0.8~1.6.
4. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 2, which is characterized in that the sylvite packet
Include potassium chloride, potassium sulfate, potassium nitrate, potassium carbonate and saleratus, the potassium chloride, potassium sulfate, potassium nitrate, potassium carbonate and
The mass ratio of the mixture of saleratus is 3~4：2.5~3：2~3：1.5~2.5：1~2.5.
5. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 2, which is characterized in that the microcosmic salt packet
Include calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate, the calcium phosphate, magnesium phosphate, ferric phosphate and trbasic zinc phosphate mass ratio be
1.5~4：1.5~4：1~3.5：1~3.5.
6. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 1, which is characterized in that the vitamin
A concentration of 0.5~0.8g/100ml, a concentration of 0.2~0.8g/100ml of the amino acid.
7. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 1, which is characterized in that the chitin
A diameter of 200~500nm of nanofiber.
8. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 1, which is characterized in that the plant source
Antibacterial growth-promoting elongated biological pesticide is applied to Agrobacterium tumefaciens, bacillus subtilis, Escherichia coli, avenae subsp.citrull, eucalyptus
Set ralstonia solanacearum, tomato Streptomyces scabies and ash arrhizus bacteria.
9. the antibacterial growth-promoting elongated biological pesticide of a kind of plant source according to claim 1, which is characterized in that the oleander
The mass ratio of oleander extract, sour leaf glue boisiana extract and goat's horn col extract is 1~5 in section's extract：2~3：1.5
~3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810281364.XA CN108341716A (en) | 2018-04-02 | 2018-04-02 | A kind of antibacterial growth-promoting elongated biological pesticide of plant source |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810281364.XA CN108341716A (en) | 2018-04-02 | 2018-04-02 | A kind of antibacterial growth-promoting elongated biological pesticide of plant source |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108341716A true CN108341716A (en) | 2018-07-31 |
Family
ID=62957731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810281364.XA Pending CN108341716A (en) | 2018-04-02 | 2018-04-02 | A kind of antibacterial growth-promoting elongated biological pesticide of plant source |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108341716A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250188A (en) * | 2008-03-25 | 2008-08-27 | 西安皓天生物工程技术有限责任公司 | Technique for preparing willow leaf tabersonine |
| CN102140060A (en) * | 2011-02-24 | 2011-08-03 | 黄仁彬 | Method for extracting and separating crude extracts of carambola root quinones chemical components |
| CN103127191A (en) * | 2013-03-20 | 2013-06-05 | 新疆艾比湖戈宝麻有限公司 | Preparation method and application of apocynum venetum extract |
| CN105777414A (en) * | 2016-03-31 | 2016-07-20 | 太仓市新进农场专业合作社 | Pest-resistant liquid fertilizer containing traditional Chinese medicine extracting liquor |
| CN105994408A (en) * | 2016-05-13 | 2016-10-12 | 铜梁县纯农水果种植股份合作社 | Composition for preventing phytophthora rot of Huangguan pears and application method of composition |
| CN106116865A (en) * | 2016-06-24 | 2016-11-16 | 安徽省安庆市金太阳生物科技有限责任公司 | A kind of sustained release fertilizer additive rich in trace element and preparation method thereof |
| CN106188213A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of Simple Purification method of tea saponin |
| CN106495862A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of composite pesticide for apple tree cultivation and preparation method thereof |
| CN106490035A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | One grows tobacco special Anti-bacterium agricultural chemicals and preparation method thereof |
| CN106748116A (en) * | 2016-12-16 | 2017-05-31 | 天峨县全盛蜂业科技有限公司 | A kind of foliar fertilizer |
| CN107646893A (en) * | 2017-09-28 | 2018-02-02 | 胡静 | A kind of plants and flowers plant source efficient pesticides and preparation method thereof |
-
2018
- 2018-04-02 CN CN201810281364.XA patent/CN108341716A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250188A (en) * | 2008-03-25 | 2008-08-27 | 西安皓天生物工程技术有限责任公司 | Technique for preparing willow leaf tabersonine |
| CN102140060A (en) * | 2011-02-24 | 2011-08-03 | 黄仁彬 | Method for extracting and separating crude extracts of carambola root quinones chemical components |
| CN103127191A (en) * | 2013-03-20 | 2013-06-05 | 新疆艾比湖戈宝麻有限公司 | Preparation method and application of apocynum venetum extract |
| CN105777414A (en) * | 2016-03-31 | 2016-07-20 | 太仓市新进农场专业合作社 | Pest-resistant liquid fertilizer containing traditional Chinese medicine extracting liquor |
| CN105994408A (en) * | 2016-05-13 | 2016-10-12 | 铜梁县纯农水果种植股份合作社 | Composition for preventing phytophthora rot of Huangguan pears and application method of composition |
| CN106116865A (en) * | 2016-06-24 | 2016-11-16 | 安徽省安庆市金太阳生物科技有限责任公司 | A kind of sustained release fertilizer additive rich in trace element and preparation method thereof |
| CN106188213A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of Simple Purification method of tea saponin |
| CN106495862A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of composite pesticide for apple tree cultivation and preparation method thereof |
| CN106490035A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | One grows tobacco special Anti-bacterium agricultural chemicals and preparation method thereof |
| CN106748116A (en) * | 2016-12-16 | 2017-05-31 | 天峨县全盛蜂业科技有限公司 | A kind of foliar fertilizer |
| CN107646893A (en) * | 2017-09-28 | 2018-02-02 | 胡静 | A kind of plants and flowers plant source efficient pesticides and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 万树青: "《生物农药及使用技术》", 31 January 2003, 金盾出版社 * |
| 孔令义: "《中药制药化学》", 30 April 2007, 中国医药科技出版社 * |
| 林亚平 等: "《中药民族药质量标准研究专论》", 31 December 2008, 贵州科技出版社 * |
| 林锦明: "《化学实验室工作手册》", 31 January 2016, 第二军医大学出版社 * |
| 高祥照 等: "《化肥手册》", 30 September 2000, 中国农业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102145970B (en) | Multifunctional substrate modifier for aquaculture and preparation method thereof | |
| Garland et al. | Plant-mediated rhizospheric interactions in maize-pigeon pea intercropping enhance soil aggregation and organic phosphorus storage | |
| CA2420893A1 (en) | Method of obtaining a hydroxytyrosol-rich composition from vegetation water | |
| CN104557312A (en) | High-nutrient microbial bacterium composite fertilizer and production method thereof | |
| CN101050180A (en) | Method for preparing chlorogenic acid, insecticide, bactericide, and charcoal from boneset with purple stem | |
| CN105457061A (en) | Natural plant deodorant and preparation process thereof | |
| CN102285842A (en) | Tebuconazole-algae sustained-release microcapsule bactericide and preparation method thereof | |
| CN103011973A (en) | Manufacture method of synergic type nitrogen-phosphorus-potassium mixed fertilizer with slow release and loss control effect | |
| Gholami-Shabani et al. | Green nanotechnology: biomimetic synthesis of metal nanoparticles using plants and their application in agriculture and forestry | |
| CN103011971A (en) | Manufacturing method of compound organic fertilizer with fertilizer-retaining and loss-controlling functions | |
| CN108341716A (en) | A kind of antibacterial growth-promoting elongated biological pesticide of plant source | |
| CN109287665A (en) | A kind of ginkgolic acid-avermectin composite insecticide and preparation method thereof | |
| CN101302156B (en) | Process for extracting chlorogenic acid from Eupatorium adenophorum | |
| CN101036475A (en) | Nuisanceless high-efficient vegetal pesticide | |
| CN113509409B (en) | Preparation method and application of galangal seed flower essential oil | |
| CN106577774A (en) | Production method of marine animal pesticide | |
| CN101129324B (en) | Secondary raffinate product of dunaliella salina and application of the same in daily product of chemical industry for skin health care | |
| CN106916034A (en) | A kind of biomass composite and its preparation technology and application | |
| Sulewska et al. | The use of microorganisms as bio-fertilizers in the cultivation of white lupine | |
| CN109169726A (en) | A kind of compounding agent containing bactericidal effect | |
| CN108566963A (en) | A kind of vegetable cultivation vegetable insecticide and preparation method thereof | |
| CN108101689A (en) | Activated sludge organic biofertilizer and preparation method thereof | |
| CN107771866A (en) | A kind of crowndaisy chrysanthemum bacteriostatic activity monomer and its application method | |
| Carrazoni et al. | Mechanism of the entomotoxic activity induced by Araucaria angustifolia methanolic extract in Nauphoeta cinerea lobster cockroaches | |
| CN106116724A (en) | A kind of polygonum multiflorum planting fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180731 |
|
| RJ01 | Rejection of invention patent application after publication |