CN109169726A - A kind of compounding agent containing bactericidal effect - Google Patents
A kind of compounding agent containing bactericidal effect Download PDFInfo
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- CN109169726A CN109169726A CN201811016995.5A CN201811016995A CN109169726A CN 109169726 A CN109169726 A CN 109169726A CN 201811016995 A CN201811016995 A CN 201811016995A CN 109169726 A CN109169726 A CN 109169726A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/08—Amines; Quaternary ammonium compounds containing oxygen or sulfur
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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Abstract
The invention discloses a kind of compounding agent containing bactericidal effect, the principle active component of the compounding agent is camphor tree essential oil and ephedrine；Camphor tree essential oil is prepared using with 2- methylalanine and calcium citrate collaboration distillation extractive technique, prepares ephedrine using n,N-Dimethylglycine and zinc acetate collaboration ultrasonic wave added zymolysis technique, products therefrom high income, purity are good.The present invention is the compounding agent that phytochemical camphor tree essential oil and chemical agent ephedrine effective component carry out binary built with 1:20-20:1, and 1:5-5:1 compounding agent synergy is obvious, and bacteriostatic activity is high.Have the beneficial effect that camphor tree essential oil of the present invention and ephedrine compounding agent can be made into the preparations such as missible oil, aqueous emulsion, suspoemulsion, water dispersible granules, for preventing and treating the diseases such as crop gray mold, head blight, virosis, leaf mold, brown spot, ring spot, wilt, epidemic disease.
Description
Technical field
The present invention relates to pesticide sterilizing composite fields, more particularly, to a kind of compounding agent containing bactericidal effect.
Background technique
As society is increasingly to the attention of environmental protection, a large amount of uses of high poison, persistent pesticide are to agricultural production and life
It is negatively affected caused by state environment and has caused worldwide extensive concern.Forbid and limit a large amount of high poisons, persistent pesticide
It uses, exploitation and the important subject that protection environment and promotion agricultural development are had become using biogenic pesticide.Therefore it develops
The favor that with environment there is compatibility, the biological pesticide resource harmless to the mankind to be increasingly subject to various countries with utilization.
Camphor tree also known as cinnamomum camphora, black camphor tree, camphorwood, fragrant camphor tree etc., are the evergreen tall and big broad-leaved arbors of Lauraceae Cinnamomum.Camphor tree is in China
It is widely distributed, it is important economic tree and green tree species.Camphor tree is natural perfume material tree species, the portions such as its root, stem, leaf, fruit
Position, which can refine, isolates essential oil.Essential oil is the product of secondary metabolism in camphor tree metabolic processes, gene genetic expression,
Habitat collective effect will lead to it and generate complicated hereditary variation.The chemical component of camphor tree essential oil is very abundant, having more than ingredient
Several hundred kinds, it is few also have it is ten several.Pelissier etc. researchs and analyses the essential oil of camphortree leaf, stem and skin, obtain 52 kinds of chemistry at
Point, main component is camphor and cineol, and Zhang Guofang etc. has carried out system point to 329 parts of camphortree leaf Chemical Composition of The Essential Oil contents
Analysis therefrom selects 21, australene, β-phellandrene, 1,8- cineole, linalool, camphor, epoxylinalol, borneol etc. averagely
The higher chemical component of relative amount accounts for the 90.74% of the total chemical composition content of camphor tree leaf essential oil.Most ofization of camphor tree essential oil
Studying point plays the role of sterilization, antibacterial, in the widely used of the industries such as light industry, perfumery industry, beauty culture, pharmaceutical sector, has
Wide exploitation prospect.
Chinese ephedra is the drying herbaceous stem stem of Ephedraceae plant ephedra sinica, epheday intermedia or ephedra equisetina, originates in China Hebei, mountain
The ground such as west, Shaanxi, Gansu and Inner Mongol.The effect of its is warm-natured, and acrid flavour, slight bitter have inducing sweat and dispelling exogenous evils, freeing lung and relieving asthma, inducing diuresis for removing edema.Fiber crops
The ingredients such as yellow class, flavones and tannin class containing alkaloids, volatile oil, carbohydrate, organic acid and other class compounds, wherein
Mainly based on ephedrine hydrochloride and pseudoephedrine hydrochloride.Ephedrine has antibacterial bacteriostatic, antiviral, and excitor nerve maincenter is made
With being widely used in field of medicaments.
Although camphor tree essential oil and ephedrine have good bacteriostatic activity, in control of agricultural pest application, at present
It is still rarely reported, and the two is used in compounding, have no that pertinent literature is reported.Camphor tree essential oil and ephedrine are that plant source is living
Property substance, have Environmental compatibility, meet the developing trend of public nuisance-free agricultural chemicals product.The present invention is by by camphor tree essential oil and Chinese ephedra
Alkali compounding, is found to have certain synergistic function, is used for control of crop disease, and control efficiency can be improved, and reduces dosage,
Spraying times are reduced, prevention and treatment range is expanded, meet the requirement of decrement pesticide application technology at present.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation method is simple, essential oil and ephedrine purity is high, extraction efficiency are high
Built disinfectant containing camphor tree essential oil and ephedrine, built disinfectant high-efficiency low-toxicity obtained, low-residual, Environmental compatibility
It is high.
The present invention in view of the above technology in the problem of mentioning, the technical solution taken are as follows:
A kind of compounding agent containing bactericidal effect, effective component include camphor tree essential oil and ephedrine；
The preparation of camphor tree essential oil: Camphor Tree Seeds, leaf and skin clean up, and dry, and are cut to 2-3cm, and bruisher crushes
Afterwards, then with Ultra-Micro Grinding Equipment it crushes, Jing≤12 μm Li of crushing；By weight, 100 parts of camphor tree Ultramicro-powders, 5-15 parts are taken
2- methylalanine, 5-15 part calcium citrate, 300-500 parts of distilled water are added in flask, install distilling apparatus, heating distillation
Oil and water after distillation, are separated by oil water separator, collect essential oil with brown reagent bottle by 3-6h；2- methylalanine
With the presence of calcium citrate, can be destroyed with permeation cell wall by chemical affinities such as hydrogen bond, Van der Waals force, electrostatic attractions
Cell wall and membrane structure promote intracellular protein, antibacterial material to change cell wall and permeability of cell membranes
Release, while ultramicro grinding and distillation technique are cooperateed with, it is isocellular thin greatly to destroy Camphor Tree Seeds, leaf, skin for mutual cooperative gain
Cell wall, and accelerate the movement of intracellular molecules, camphor tree essential oil is promoted to discharge and spread the purity for improving essential oil in the solution and mention
Take rate；
The preparation of ephedrine: it in parts by weight, using 100 parts of Herba Ephedraes as raw material, cleans up, dries, bruisher is thick
It crushes, crosses 60-100 mesh, water 400-600 parts, 5-15 parts of n,N-Dimethylglycine, 5-15 parts of zinc acetate, tan is then added
1.5-3 parts of matter enzyme, 2-10 parts of cellulase, 1-3 parts of pectase, 1-3 parts of protease adjust pH value 4.0-5.5, temperature 35-55
DEG C, 100-150W ultrasonic wave added digests 1-3h, and boiling water enzyme deactivation is living, centrifuging and taking supernatant, and it is 12- that adding sodium hydroxide, which alkalizes to pH,
14, three times with the chloroform recovery isometric with supernatant, combined extract is concentrated under reduced pressure, dry, obtains ephedrine；N, N- diformazan
The presence of base glycine and zinc acetate can form the chemical affinities such as hydrogen bond with molecular structure on the outside of cell wall, destroy cell wall,
Promote intracellular matter to discharge and precipitate the tannin in Herba Ephedrae, promotes the enzyme of tannin enzyme, cellulase, pectase and protease
Solution effect, further promotes the release and dissolution of ephedrine sulfate.
Preferably, the weight ratio of camphor tree essential oil and ephedrine is 1:20-20:1 in the compounding agent obtained, it is further excellent
It selects, the weight ratio of camphor tree essential oil and ephedrine is 1:5-5:1 in the compounding agent obtained；Camphor tree essential oil is grown to mycelia and spore
Sprouting has strong inhibiting effect, and under a certain concentration range, the organelle of fungi is smudgy, dissolution disappears；Carry out
When camphor tree essential oil tests the crop diseases such as ring rot of apple, wheat scab, discovery camphor tree essential oil single dose in use cannot
The occurrence and development of these germs are effectively controlled, i.e., cannot give full play to its drug effect, for the disease control spectrum for expanding camphor tree essential oil, are led to
It crosses and is compounded with other bacteriostatic agents, bacteriostatic test is carried out to common disease fungus；It has passed through a large amount of experiment sieving compound preparation,
It was found that with camphor tree essential oil and Chinese ephedra alkali composition to for the gray mold of examination, head blight, virosis, leaf mold, brown spot, wheel line
Disease, wilt, epidemic disease have better control efficiency；The proportion screening discovery effective component of two kinds of compounds of further progress
Camphor tree essential oil and synergistic component ephedrine, weight ratio are that 1:20-20:1 has synergistic effect, wherein preferred weight ratio is 1:
5-5:1。
Preferably, the compounding agent can be prepared into missible oil, aqueous emulsion, suspoemulsion or water dispersible granules；The experiment proved that
Above-mentioned compounding agent can be used for preparing prevention and treatment crop gray mold, head blight, virosis, leaf mold, brown spot, ring spot, wither
The application of pesticide in germ or epidemic disease disease.
Preferably, the composition and weight percentage of made missible oil are as follows: camphor tree essential oil: 1-50%, ephedrine: 0.5-
30%, antioxidant: 0.2-2%, emulsifier: 5-20%, solvent polishing to 100%；Wherein:
Antioxidant used be one of dibutyl hydroxy toluene, butylated hydroxy anisole or ascorbic acid substance or
A variety of mixtures；
Solvent for use is N,N-dimethylformamide, toluene, methylene chloride, ethyl acetate or isopropanol；
Emulsifier used is alkyl polyoxyethylene ether, polyoxyethylene carboxylate, polyglyceryl fatty acid ester, citric acid fat
Acid glyceride, sorbester p17, sorbester p18, OP-10, No. 500,602 or one of M30 or a variety of mixtures.
Its preparation process are as follows: first antioxidant is put into the mixing mother liquor of quantitative weighed camphor tree essential oil and ephedrine and filled
Divide and mix thoroughly, then again mixes well the weighed solvent of accurate quantitative analysis and emulsifier, finally by above two solution in preparing tank
Middle mixing simultaneously continues stirring 2~4 hours, and product is sub-packed in plastic bottle, sealing storage.
Preferably, when aqueous emulsion is made, the composition and weight percentage of made aqueous emulsion are as follows: camphor tree essential oil 1-
20%, ephedrine 1-10%, emulsifier 10-20%, solvent 5-25%, antioxidant 1-3%, deionized water polishing to 100%,
Wherein:
Emulsifier used is ethylene oxide propylene oxide block copolymer, alkyl phenyl polyglycol ether, fatty acid polyglycol
Ethylene oxide ester, phenethyl phenol polyethenoxy ether, alkyl polyoxyethylene ether, polyethylene glycol hydrogenated castor-oil plant RH40, one in lecithin
Kind or a variety of mixtures；
Solvent used is one of toluene, methanol, ethyl alcohol, ethyl acetate, isopropanol or a variety of mixtures；
Antioxidant used be one of dibutyl hydroxy toluene, butylated hydroxy anisole or ascorbic acid substance or
A variety of mixtures；
Its preparation process are as follows: first antioxidant is put into the mixing mother liquor of quantitative weighed camphor tree essential oil and ephedrine and filled
Divide and mix thoroughly, the weighed emulsifier of accurate quantitative analysis and solvent are mixed well, finally above two solution is used in combination in preparing tank
Water polishing persistently stirs 2~4 hours, product is sub-packed in vial, sealing storage to corresponding weight.
Preferably, when suspoemulsion is made, the composition and weight percentage of the suspoemulsion are as follows: camphor tree essential oil 1-20%,
Ephedrine 1-10%, dispersing agent 2-5%, wetting agent 1-3%, emulsifier 2-8%, antisettling agent 1-4%, antifreezing agent 1-5% disappear
Infusion 0.2-2%, antioxidant 2-5%, deionized water polishing to 100%, in which:
Dispersing agent used is polycarboxylate salt dispersant, triphenyl vinyl phenol polyoxyethylene ether phosphate, castor oil polyoxy
Ethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, lignosulfonates, polyoxyethylene polyoxypropylene base ether block are total
One of polymers or a variety of mixtures；
Wetting agent used is alkyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, one in alkylnaphthalene sulfonate
Kind or a variety of mixtures；
Emulsifier used is ethylene oxide propylene oxide block copolymer, alkyl phenyl polyglycol ether, fatty acid polyglycol
One of ethylene oxide ester, polyethylene glycol hydrogenated castor-oil plant RH40, lecithin or a variety of mixtures；
Antifreezing agent used is one of isopropanol, glycerine, butanol or a variety of mixtures；
Antisettling agent used is one of xanthan gum, aluminum magnesium silicate, polyvinyl alcohol or carboxymethyl cellulose or a variety of
Mixture；
Defoaming agent used is organic silicon defoamer；
Its preparation process are as follows: in the mixing mother liquor of camphor tree essential oil and ephedrine, be separately added into dispersing agent, wetting agent, cream
Agent, antifreezing agent, antisettling agent and defoaming agent, finally with water polishing to predetermined weight.
Preferably, when water dispersible granules are made, water dispersible granules composition and weight percentage are as follows: camphor tree essential oil is
1-20%, ephedrine 1-10%；Dispersing agent 2-5%, wetting agent 1-3%, adhesive 1-3%, disintegrating agent 1-3%, carrier are mended
Together to 100%；Wherein:
Dispersing agent used is polycarboxylate salt dispersant, triphenyl vinyl phenol polyoxyethylene ether phosphate, castor oil polyoxy
Ethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, lignin sulfonic acid sodium salt, polyoxyethylene polyoxypropylene base ether block
One of copolymer or a variety of mixtures；
Wetting agent used is alkyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, one in alkylnaphthalene sulfonate
Kind or a variety of mixtures；
Adhesive used is soluble starch, DEXTROSE ANHYDROUS, polyvinyl alcohol, polyethylene glycol or sodium carboxymethylcellulose
One of or a variety of mixtures；
Disintegrating agent used is one of sodium chloride, urea, bentonite, ammonium sulfate or a variety of mixtures；
Carrier used is one of diatomite, talcum powder, clay, bentonite, kaolin, white carbon black, precipitated calcium carbonate
Or a variety of mixtures；
Its preparation process are as follows: mix camphor tree essential oil, ephedrine, dispersing agent, wetting agent, adhesive, disintegrating agent and carrier
It is crushed after uniformly, after remixing uniformly plus water mediate and extruding pelletization through drying, whole grain, screening it is equal to obtain appearance
Even, complete mustard essential oil and allicin water dispersible granules qualified products.
Compared with the prior art, the advantages of the present invention are as follows: the present invention is utilized to be assisted with 2- methylalanine and calcium citrate
Camphor tree essential oil is prepared with distillation extractive technique, cooperates with ultrasonic wave added zymolysis technique system using n,N-Dimethylglycine and zinc acetate
Standby ephedrine, made camphor tree essential oil and ephedrine yield are high, with high purity；The camphor tree essential oil and ephedrine that the present invention uses are natural
Plants antimicrobial active material has inhibiting effect to various bacteria, fungi and virus, with Environmental compatibility is good, it is anti-to be not likely to produce
Pharmacological property uses safe advantage；It by the compounding of camphor tree essential oil and ephedrine, has significant synergies, to various crop
The diseases such as gray mold, head blight, virosis, leaf mold, brown spot, ring spot, wilt or epidemic disease have good prevention and treatment to imitate
Fruit, cardinal principle are using camphor tree essential oil as main active, using ephedrine as synergistic component；Pass through camphor tree essential oil and Chinese ephedra
Aqueous emulsion made from the synergistic complex agent of alkali, suspoemulsion or water dispersible granules stable product quality, it is environmental-friendly, it is easy to use,
It is suitable for promoting.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A kind of compounding agent containing bactericidal effect, effective component include camphor tree essential oil and ephedrine；
The preparation of camphor tree essential oil: Camphor Tree Seeds, leaf and skin clean up, and dry, and are cut to 2-3cm, and bruisher crushes
Afterwards, then with Ultra-Micro Grinding Equipment it crushes, Jing≤12 μm Li of crushing；By weight, 100 parts of camphor tree Ultramicro-powders, 10 parts of 2- are taken
Methylalanine, 10 parts of calcium citrates, 400 parts of distilled water are added in flask, install distilling apparatus, and heating distillation 5h has distilled
Oil and water are separated by oil water separator, collect essential oil with brown reagent bottle by Bi Hou；
The preparation of ephedrine: it in parts by weight, using 100 parts of Herba Ephedraes as raw material, cleans up, dries, bruisher is thick
Crush, cross 60-100 mesh, then be added 500 parts of water, 10 parts of n,N-Dimethylglycine, 10 parts of zinc acetate, 2 parts of tannin enzyme,
7 parts of cellulase, 2 parts of pectase, 2 parts of protease adjust pH value 5.0, and temperature is 50 DEG C, and 120W ultrasonic wave added digests 2h, boiling water
Enzyme deactivation is living, centrifuging and taking supernatant, and it is 14 that adding sodium hydroxide, which alkalizes to pH, three times with the chloroform recovery isometric with supernatant, closes
And extracting solution, it is concentrated under reduced pressure, it is dry, obtain ephedrine；
Camphor tree essential oil and ephedrine compounded cream agent prescription: by weight percentage: camphor tree essential oil: 20%, ephedrine:
4%, antioxidant: 1%, emulsifier: 10%, solvent polishing to 100%；
Preparation method: the quantitatively mother liquor of weighed camphor tree essential oil and ephedrine being put into blend tank and is mixed evenly,
Quantitative weighed dibutyl hydroxy toluene is added, is again stirring for uniformly；It is accurate to measure ethyl acetate and sorbester p17, it is added to allotment
In tank, persistently stirs 3 hours, obtain camphor tree essential oil and ephedrine compounded cream agent product, be sub-packed in plastic bottle, sealing storage.
Embodiment 2:
A kind of compounding agent containing bactericidal effect, effective component include camphor tree essential oil and ephedrine；
The preparation of camphor tree essential oil: Camphor Tree Seeds, leaf and skin clean up, and dry, and are cut to 2-3cm, and bruisher crushes
Afterwards, then with Ultra-Micro Grinding Equipment it crushes, Jing≤12 μm Li of crushing；By weight, 100 parts of camphor tree Ultramicro-powders, 5 parts of 2- first are taken
Base alanine, 5 parts of calcium citrates, 300 parts of distilled water are added in flask, install distilling apparatus, heating distillation 3h, and distillation finishes
Afterwards, oil and water are separated by oil water separator, collects essential oil with brown reagent bottle；
The preparation of ephedrine: it in parts by weight, using 100 parts of Herba Ephedraes as raw material, cleans up, dries, bruisher is thick
Crush, cross 60-100 mesh, then be added 400 parts of water, 5 parts of n,N-Dimethylglycine, 5 parts of zinc acetate, 1.5 parts of tannin enzyme,
2 parts of cellulase, 1 part of pectase, 1 part of protease adjust pH value 4.0, and temperature is 35 DEG C, and 100W ultrasonic wave added digests 1h, boiling water
Enzyme deactivation is living, centrifuging and taking supernatant, and it is 12 that adding sodium hydroxide, which alkalizes to pH, three times with the chloroform recovery isometric with supernatant, closes
And extracting solution, it is concentrated under reduced pressure, it is dry, obtain ephedrine；
Camphor tree essential oil compounds water emulsion formula with ephedrine: by weight percentage: camphor tree essential oil 2%, ephedrine 10%,
Emulsifier 15%, solvent 20%, antioxidant 2%, deionized water polishing to 100%；
Preparation method: the quantitatively mother liquor of weighed camphor tree essential oil and ephedrine being put into blend tank and is mixed evenly,
Quantitative weighed butylated hydroxy anisole is added, is again stirring for uniformly；It is accurate to measure isopropanol and phenethyl phenol polyethenoxy ether,
It is added in blend tank, persistently stirs 3 hours, obtain mustard essential oil and compound water emulsion formula product with allicin, be sub-packed in plastics
In bottle, sealing storage.
Embodiment 3:
A kind of compounding agent containing bactericidal effect, effective component include camphor tree essential oil and ephedrine；
The preparation of camphor tree essential oil: Camphor Tree Seeds, leaf and skin clean up, and dry, and are cut to 2-3cm, and bruisher crushes
Afterwards, then with Ultra-Micro Grinding Equipment it crushes, Jing≤12 μm Li of crushing；By weight, 100 parts of camphor tree Ultramicro-powders, 15 parts of 2- are taken
Methylalanine, 15 parts of calcium citrates, 500 parts of distilled water are added in flask, install distilling apparatus, and heating distillation 6h has distilled
Oil and water are separated by oil water separator, collect essential oil with brown reagent bottle by Bi Hou；
The preparation of ephedrine: it in parts by weight, using 100 parts of Herba Ephedraes as raw material, cleans up, dries, bruisher is thick
Crush, cross 60-100 mesh, then be added 600 parts of water, 15 parts of n,N-Dimethylglycine, 15 parts of zinc acetate, 3 parts of tannin enzyme,
10 parts of cellulase, 3 parts of pectase, protease 3 part adjust pH value 5.5, and temperature is 55 DEG C, and 150W ultrasonic wave added digests 3h, boiling water
Enzyme deactivation is living, centrifuging and taking supernatant, and it is 14 that adding sodium hydroxide, which alkalizes to pH, three times with the chloroform recovery isometric with supernatant, closes
And extracting solution, it is concentrated under reduced pressure, it is dry, obtain ephedrine；
Camphor tree essential oil compounds suspoemulsion formula with ephedrine: by weight percentage: camphor tree essential oil 20%, ephedrine 4%,
Dispersing agent 4%, wetting agent 2%, emulsifier 5%, antisettling agent 3%, antifreezing agent 3%, defoaming agent 1%, antioxidant 3% are gone
Ionized water polishing is to 100%；
Preparation method: the quantitatively mother liquor of weighed camphor tree essential oil and ephedrine being put into blend tank and is mixed evenly,
Sequentially add quantitatively weighed dispersing agent, wetting agent, emulsifier, antifreezing agent, antisettling agent, defoaming agent, extremely with water polishing finally
Predetermined weight obtains mustard essential oil and compounds suspoemulsion formulation product with allicin.Packing, sealing storage.
Embodiment 4:
A kind of compounding agent containing bactericidal effect, effective component include camphor tree essential oil and ephedrine；
The preparation of camphor tree essential oil: Camphor Tree Seeds, leaf and skin clean up, and dry, and are cut to 2-3cm, and bruisher crushes
Afterwards, then with Ultra-Micro Grinding Equipment it crushes, Jing≤12 μm Li of crushing；By weight, 100 parts of camphor tree Ultramicro-powders, 12 parts of 2- are taken
Methylalanine, 10 parts of calcium citrates, 350 parts of distilled water are added in flask, install distilling apparatus, and heating distillation 4h has distilled
Oil and water are separated by oil water separator, collect essential oil with brown reagent bottle by Bi Hou；
The preparation of ephedrine: it in parts by weight, using 100 parts of Herba Ephedraes as raw material, cleans up, dries, bruisher is thick
Crush, cross 60-100 mesh, then be added 500 parts of water, 12 parts of n,N-Dimethylglycine, 12 parts of zinc acetate, 2 parts of tannin enzyme,
8 parts of cellulase, 2 parts of pectase, 2 parts of protease adjust pH value 4.5, and temperature is 45 DEG C, and 130W ultrasonic wave added digests 2h, boiling water
Enzyme deactivation is living, centrifuging and taking supernatant, and it is 13h that adding sodium hydroxide, which alkalizes to pH, three times with the chloroform recovery isometric with supernatant, closes
And extracting solution, it is concentrated under reduced pressure, it is dry, obtain ephedrine；
Camphor tree essential oil and ephedrine compound water dispersible granule formula: by weight percentage: camphor tree essential oil is 10%, Chinese ephedra
Alkali is 5%；Dispersing agent 3%, wetting agent 2%, adhesive 2%, disintegrating agent 2%, carrier polishing to 100%；
Preparation method: the camphor tree essential oil accurately weighed, ephedrine and the above auxiliary agent and carrier are subjected to powder after mixing
Broken, after remixing uniformly plus water mediate and extruding pelletization through drying, whole grain, screening obtains appearance uniform, complete mustard
Last essential oil and allicin water dispersible granules qualified products.
Comparative example 1:
2- methylalanine and calcium citrate are not added in camphor tree essential oil preparation step, rest part and embodiment 1 are complete
Unanimously.
Comparative example 2:
N,N-Dimethylglycine, zinc acetate, rest part and embodiment 1 complete one are not added in ephedrine preparation step
It causes.
Embodiment 4:
It regard embodiment 1 as test group, comparative example 1, comparative example 21, control group 2 as a control group, camphor tree essential oil and Chinese ephedra
The yield of alkali is shown in Table 1.
It is weighed with assay balance, and calculated according to the following formula:
Yield (%)=camphor tree essential oil quality (g)/camphortree leaf quality (g) × 100% of camphor tree essential oil；
The yield (%) of ephedrine=extraction ephedrine quality (g)/ephedrine gross mass (g) × 100%.
The yield of table 1 camphor tree essential oil and ephedrine
| Project | Camphor tree essential oil yield (%) | Ephedrine yield (%) |
| Test group | 1.30 | 95 |
| Control group 1 | 0.89 | 95 |
| Control group 2 | 1.30 | 73 |
As shown in Table 1, the yield of test group camphor tree essential oil is higher than control group 1, illustrates to add 2- methylalanine and lemon
Sour calcium can effectively destroy cell wall, improve the recovery rate of camphor tree essential oil；The yield of test group ephedrine is higher than control group 2, explanation
N,N-Dimethylglycine, zinc acetate are added, the recovery rate of ephedrine can be effectively improved.
Embodiment 5:
By compound preparation missible oil, aqueous emulsion, suspoemulsion and the water of camphor tree essential oil prepared by above-described embodiment and ephedrine
Dispersible granule carries out the effect experiment of prevention and treatment ring rot of apple in field, and comparison medicament is 50% carbendazol wettable powder, examination
It tests and the results are shown in Table 2.
2 camphor tree essential oil of table and ephedrine compound preparation prevention and treatment ring rot of apple field control effectiveness test result (Yantai)
Table 2 the results show that the compound preparation of camphor tree essential oil and ephedrine there is fabulous prevention and treatment to imitate ring rot of apple
Fruit reaches 90% or more control efficiency for 3 days after application, reaches within 7 days after application 95% or more control efficiency, be higher than pair
According to the control efficiency of 50% carbendazol wettable powder of medicament.
Embodiment 6:
By compound preparation missible oil, aqueous emulsion, suspoemulsion and the water of camphor tree essential oil prepared by above-described embodiment and ephedrine
Dispersible granule carries out the effect experiment of prevention and treatment wheat scab in field, and comparison medicament is 70% thiophanate methyl wettable powder
Agent, test result are shown in Table 3.
3 camphor tree essential oil of table and ephedrine compound preparation prevention and treatment wheat scab field control effectiveness test result (Pingyi)
Table 3 the results show that the compound preparation of camphor tree essential oil and ephedrine there is fabulous prevention and treatment to imitate wheat scab
Fruit reaches 90% or more control efficiency for 6 days after application, anti-higher than 70% thiophanate methyl wettable powder of comparison medicament
Control effect.
Routine operation in operating procedure of the invention is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of compounding agent containing bactericidal effect, which is characterized in that effective component includes camphor tree essential oil and ephedrine.
2. a kind of compounding agent containing bactericidal effect as described in claim 1, which is characterized in that the preparation of the camphor tree essential oil
Method are as follows: by Camphor Tree Seeds, leaf and skin, clean up, dry, be cut to 2-3cm, coarse crushing is pulverized to the μ of Li Jing≤12
m；By weight, 100 parts of camphor tree Ultramicro-powders, 5-15 parts of 2- methylalanines, 5-15 parts of calcium citrates, 300-500 parts of steamings are taken
Distilled water is heated using distilling apparatus and is distilled, and is then separated oil and water by oil water separator, collects essence with brown reagent bottle
Oil.
3. a kind of compounding agent containing bactericidal effect as described in claim 1, which is characterized in that the preparation side of the ephedrine
Method are as follows: 100 parts of Herba Ephedraes are taken, cleans, dries, coarse crushing, cross 60-100 mesh, 400-600 parts of water, 5-15 parts of N are then added,
N- dimethylglycine, 5-15 part zinc acetate, 1.5-3 parts of tannin enzymes, 2-10 parts of cellulases, 1-3 parts of pectases, 1-3 portions of eggs
White enzyme adjusts pH value to 4.0-5.5, and temperature is 35-55 DEG C, and ultrasonic wave added enzymatic hydrolysis, boiling water enzyme deactivation is living, and centrifuging and taking supernatant adds hydrogen
Sodium oxide molybdena alkalization, three times, combined extract is concentrated under reduced pressure chloroform recovery, dry, obtains ephedrine.
4. a kind of compounding agent containing bactericidal effect as described in claim 1, which is characterized in that the camphor tree essential oil and Chinese ephedra
The weight ratio of alkali is 1:20-20:1.
5. a kind of compounding agent containing bactericidal effect as described in claim 1, which is characterized in that missible oil, water can be prepared into
Emulsion, suspoemulsion or water dispersible granules.
6. a kind of compounding agent containing bactericidal effect as claimed in claim 5, which is characterized in that the composition of the missible oil and again
Measure percentage composition are as follows: camphor tree essential oil: 1-50%, ephedrine: 0.5-30%, antioxidant: 0.2-2%, emulsifier: 5%-
20%, solvent polishing to 100%.
7. a kind of compounding agent containing bactericidal effect as claimed in claim 5, which is characterized in that the composition of the aqueous emulsion and
Weight percentage are as follows: camphor tree essential oil 1-20%, ephedrine 1-10%, emulsifier 10-20%, solvent 5-25%, antioxidant
1-3%, deionized water polishing to 100%.
8. a kind of compounding agent containing bactericidal effect as claimed in claim 5, which is characterized in that the composition of the suspoemulsion
And weight percentage are as follows: camphor tree essential oil 1-20%, ephedrine 1-10%, dispersing agent 2-5%, wetting agent 1-3%, emulsifier 2-
8%, antisettling agent 1-4%, antifreezing agent 1-5%, defoaming agent 0.2-2%, antioxidant 2-5%, deionized water polishing is extremely
100%.
9. a kind of compounding agent containing bactericidal effect as claimed in claim 5, which is characterized in that the water dispersible granules group
At and weight percentage are as follows: camphor tree essential oil be 1-20%, ephedrine 1-10%；Dispersing agent 2-5%, wetting agent 1-3% are sticked
Tie agent 1-3%, disintegrating agent 1-3%, carrier polishing to 100%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811016995.5A CN109169726A (en) | 2018-09-03 | 2018-09-03 | A kind of compounding agent containing bactericidal effect |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811016995.5A CN109169726A (en) | 2018-09-03 | 2018-09-03 | A kind of compounding agent containing bactericidal effect |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150333A (en) * | 2019-05-16 | 2019-08-23 | 井冈山大学 | A kind of oil camphor tree leaf essential oil bacteriostasis method |
| CN111991440A (en) * | 2020-08-24 | 2020-11-27 | 常州工学院 | Method for extracting flavonoid compounds in cinnamomum camphora by polymer aqueous two-phase extraction |
-
2018
- 2018-09-03 CN CN201811016995.5A patent/CN109169726A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150333A (en) * | 2019-05-16 | 2019-08-23 | 井冈山大学 | A kind of oil camphor tree leaf essential oil bacteriostasis method |
| CN111991440A (en) * | 2020-08-24 | 2020-11-27 | 常州工学院 | Method for extracting flavonoid compounds in cinnamomum camphora by polymer aqueous two-phase extraction |
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Application publication date: 20190111 |