CN110150333A - A kind of oil camphor tree leaf essential oil bacteriostasis method - Google Patents
A kind of oil camphor tree leaf essential oil bacteriostasis method Download PDFInfo
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- CN110150333A CN110150333A CN201910416296.8A CN201910416296A CN110150333A CN 110150333 A CN110150333 A CN 110150333A CN 201910416296 A CN201910416296 A CN 201910416296A CN 110150333 A CN110150333 A CN 110150333A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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Abstract
The invention discloses a kind of oily camphor tree leaf essential oil bacteriostasis methods, essential oil is extracted using steam distillation or Simultaneous distillation, the liquid distilled out, by water-oil separating, oily part is dry with anhydrous sodium sulfate, oily camphor tree leaf essential oil is obtained, and inhibition plant pathogenic fungi is carried out as effective component using the oily camphor tree leaf essential oil of acquisition.When the volume ratio of oily camphor tree leaf essential oil is 7: 25,80%, 90% and 61% is not less than respectively to the bacteriostasis rate of Fusarium oxysporum, Fusicoccum asculi and Phomopsis.
Description
Technical field
The present invention relates to a kind of botanical fungicide more particularly to a kind of oily camphor tree leaf essential oil bacteriostasis methods.
Background technique
Plant disease is the formidable enemy of agricultural production, counts according to FAO (Food and Agriculture Organization of the United Nation) (FAO), makes every year because of plant disease
At underproduction average loss be total output 10%~15% (Wu Chuanwan etc., 2004).Currently, the main side of controlling plant diseases
Method is chemical bactericide.But since chemical pesticide is potentially to the harm of human health, to the pollution of environment, to non-target organism
Influence and phytopathogen resistance development the problems such as so that the development of chemical pesticide receives the limitation from various aspects
(Zhao Jihong and Li Jianzhong, 2003).Since plants essential oil has from natural, comparatively safe to human body and environmental-friendly, life
Object activity multiplicity the features such as, therefore, from natural plants find wide spectrum, efficiently, low toxicity fungicide have become scientific research personnel and
The common objective of Pesticide use personnel.According to statistics, biological pesticide product is more than 100 kind in the world at present, but 90% or more
It is biological insecticides, scholarly forecast second important commercialized biological pesticide will be botanical fungicide.
Camphor tree is one of China natural perfume material tree species, and the positions such as its root, stem, leaf, fruit, which can refine, isolates essence
Oil comes.It is different according to the principal component of branches and leaves essential oil, camphor tree is further divided into five chemical type types: linalool type (fragrant camphor tree
Type), borneol type (imperial camphor tree type), cineole type (oily camphor tree type), brain camphor tree type (camphor type), different nerolidol type (different camphor tree type).
Camphor tree essential oil contains the broad spectrum antibiotic activities ingredient such as linalool, eudesmol, terpinol, anti-can inhibit a variety of thin
Bacterium is also to have certain bacteriostasis for fungi.Prior art discloses the antifungal activity (Hu Wen of different camphor tree leaf essential oil
It is outstanding；Li Guanxi；Cao Yusong；Ke's earthen table China；Zhou Shengtuan；The bacteriostatic activity and its chemical component [J] of different camphor tree leaf essential oil；Forestry science and technology is opened
Hair；06 phase in 2014), but inhibit fungi activity to be but rarely reported oily camphor tree leaf essential oil.
Summary of the invention
Goal of the invention: being directed to above-mentioned problems of the prior art, and the object of the present invention is to provide a kind of oily camphor tree leaf essences
Oily bacteriostasis method prevents and treats crop disease to substitute chemical pesticide, has the advantages that low toxicity, low-residual and environmental-friendly, is
Botanical fungicide provides wider selection.
Technical solution: in order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention are as follows:
A kind of oil camphor tree leaf essential oil bacteriostasis method, comprising the following steps:
1) it the extraction of oily camphor tree leaf essential oil: using oily camphor tree leaf as raw material, is mentioned using steam distillation or Simultaneous distillation
Essential oil is taken, the liquid distilled out, by water-oil separating, oil part is dry with anhydrous sodium sulfate, obtains oily camphor tree leaf essential oil；
2) inhibition plant pathogenic fungi is carried out as effective component using the oily camphor tree leaf essential oil that step 1) obtains.
Further, in step 1), the ratio of water to material of steam distillation is 12.5: 1mLg-1。
Further, in step 1), the ratio of water to material of Simultaneous distillation is 10: 1mLg-1, extractant material ratio is 2:
1mL·g-1, extractant is anhydrous ether, and extraction temperature is 40 DEG C.
Further, in step 2), plant pathogenic fungi is Fusarium oxysporum, Fusicoccum asculi and Phomopsis.
Further, in step 1), extraction power is 1200W, extraction time 65min.
Further, antibacterial in use, the volume ratio of oily camphor tree leaf essential oil is 1-7: 25 in step 2).
Further, the volume ratio of oily camphor tree leaf essential oil is 7: 25, to Fusarium oxysporum, Fusicoccum asculi and Phomopsis
Bacteriostasis rate be not less than 80%, 90% and 61% respectively.
Further, in step 1), the oily camphor tree leaf essential oil of extraction by steam distillation, contained chemical component includes: alkene
Class compound, alcohol compound, ester type compound, aldehyde compound, alkane derivative.
Further, in step 1), the oily camphor tree leaf essential oil that Simultaneous distillation extracts, contained chemical component includes: alkene
Hydrocarbon compound, alcohol compound, ester type compound, alkane derivative.
The utility model has the advantages that compared with prior art, oily camphor tree leaf essential oil is to Fusarium oxysporum, Fusicoccum asculi and Phomopsis
With apparent fungistatic effect.It is the brightest to the inhibitory effect of disease fungus when the volume ratio of oily camphor tree leaf essential oil is 7: 25
It is aobvious, 80%, 90% and 61% is not less than respectively to the bacteriostasis rate of 3 kinds of plant pathogenic fungis.Chemical pesticide is substituted to prevent and treat crop
Disease has the advantages that low toxicity, low-residual and environmental-friendly.Wider selection is provided for botanical fungicide.
Detailed description of the invention
Fig. 1 is the total ion current figure of the oily camphor tree leaf essential oil of extraction by steam distillation；
Fig. 2 is the fungistatic effect figure of the oily camphor tree leaf essential oil of extraction by steam distillation；
Fig. 3 is the total ion current figure for the oily camphor tree leaf essential oil that Simultaneous distillation extracts；
Fig. 4 is the fungistatic effect figure for the oily camphor tree leaf essential oil that Simultaneous distillation extracts.
Specific embodiment
The present invention is described further combined with specific embodiments below.
Strains tested used in following embodiment and culture medium are as follows:
Fusicoccum asculi (Fusicoccum aesculi), Phomopsis (Phomopsis macrospora) and sharp spore
Sickle-like bacteria (Fusarium oxysporium) is provided by Nanjing Forestry University.
Potato culture (ZPD): potato 200g, MgSO4·7H2O 1.5g, glucose 20g, KH2PO43g, agar
20g, VB10.05g, distilled water 1000mL.
The oily camphor tree leaf essential oil bacteriostatic activity of 1 extraction by steam distillation of embodiment
1) extraction of oily camphor tree leaf essential oil
Oily camphor tree leaf picks up from Jiangxi Ji'an.Using extraction by steam distillation oil camphor tree fresh leaf essential oil, ratio of water to material 12.5:
1mL·g-1, extraction power is 1200W, extraction time 65min, oil yield 1.64%, with anhydrous sodium sulfate it is dry after save
In a brown bottle, 4 DEG C of refrigerations are spare.
2) measurement of the ingredient of oily camphor tree leaf essential oil
Chromatographic column selects Elite-5MS (30m × 0.25mm × 0.25 μm) quartz capillary column.Split ratio 10: 1, sample introduction
Measure 0.5 μ L.280 DEG C of injector temperature, 50 DEG C of holding 2min of column temperature, with 3 DEG C of .min-1180 DEG C are risen to, keeps 2min, then with 8
℃·min-1240 DEG C of holding 5min are risen to, run 60min altogether.Carrier gas is He, flow velocity 1.0mLmin-1。
Mass spectral analysis condition: 180 DEG C of EI ion source temperature, 260 DEG C of interface temperature, 50~620m/z of scanning range.
GC-MS analysis is carried out to oily camphor tree leaf essential oil, obtains oily camphor tree leaf essential oil total ion current figure, as shown in Figure 1.Its chemistry at
Divide and content is as shown in table 1, identifies 37 kinds of ingredients altogether from oily camphor tree leaf essential oil, account for the 94.515% of essential oil total amount, mainization
It is other to close species are as follows: 23 kinds of alkenes compounds, account for the 24.168% of essential oil total amount；10 kinds of alcohol compound, account for essential oil total amount
68.986%；2 kinds of ester type compound, account for the 0915% of essential oil total amount；1 kind of aldehyde compound, account for the 0.008% of essential oil total amount；
1 kind of alkane derivative, account for the 0.438% of essential oil total amount.In these chemical components, 1,8- eudesmol, β-phellandrene, α-
The compounds contents such as terpinol, australene, left-handed nopinene and terpineol -4 are higher.
The chemical component and its content of the oily camphor tree leaf essential oil of 1 extraction by steam distillation of table
3) Antibacterial Activity of the oily camphor tree leaf essential oil to fungi
Oily camphor tree leaf essential oil 1,3,5,7mL and 25mL ZPD mixing inverted plate for taking extraction by steam distillation, are made oil camphor tree
Folic acid oil concentration is the ZPD culture medium of 1: 25,3: 25,5: 25 and 7: 25 (volume ratios), and is compared with pure ZPD inverted plate.Point
Pathogen inoculated by hypha block is not repeated 3 times to culture dish center, observes and take pictures after 25 DEG C of constant temperature incubation 4d, calculates bacteriostasis rate.
The pathogen bacterium colony average diameter being wherein seeded on control plate is Do, the pathogen being seeded on oil-containing camphor tree leaf essential oil plate
Bacterium colony average diameter is Dx, then
Bacteriostasis rate=(Do-Dx)/Do × 100%.
The bacteriostatic activity of the oily camphor tree leaf essential oil of 2 extraction by steam distillation of table
By Fig. 2 and table 2 it is found that oily camphor tree leaf essential oil all has one to Fusarium oxysporum, Fusicoccum asculi and Phomopsis
Fixed inhibitory effect, and with the increase of different camphor tree leaf essential oil concentration, inhibitory effect is more obvious.It is true to cause of disease when concentration 7: 25
The inhibitory effect of bacterium is the most obvious, respectively reaches 85.93%, 92.83% and to the bacteriostasis rate of 3 kinds of plant pathogenic fungis
65.30%, oily camphor tree leaf essential oil is respectively Fusarium oxysporum and Phomopsis 1.32 and 1.42 to the inhibiting rate of Fusicoccum asculi
Times.
The oily camphor tree leaf essential oil bacteriostatic activity that 2 Simultaneous distillation of embodiment extracts
1) extraction of oily camphor tree leaf essential oil
Simultaneous distillation-extraction: weighing the oily camphor tree leaf sample 30g handled well, be placed in 500mL round-bottomed flask, and 300mL is added
Distilled water is heated to slightly boiled mounted in one end of SDE method；SDE method the other end dress 60mL without
Water ether is heated in 250mL round-bottomed flask with 40 DEG C of waters bath with thermostatic control, Simultaneous distillation-extraction 3.5h, extract obtained with anhydrous
Na2SO4It is dry, anhydrous ether is removed with rotary evaporator to get the oily camphor tree leaf essential oil of faint yellow tool aromatic odor, oil yield is arrived
It is 1.95%.And 4 DEG C stored refrigerated spare.
2) measurement of the ingredient of oily camphor tree leaf essential oil
Chromatographic column selects Elite-5MS (30m × 0.25mm × 0.25 μm) quartz capillary column.Split ratio 10: 1, sample introduction
Measure 0.5 μ L.280 DEG C of injector temperature, 50 DEG C of holding 2min of column temperature, with 3 DEG C of min-1180 DEG C are risen to, keeps 2min, then with 8
℃·min-1240 DEG C of holding 5min are risen to, run 60min altogether.Carrier gas is He, flow velocity 1.0mLmin-1。
Mass spectral analysis condition: 180 DEG C of EI ion source temperature, 260 DEG C of interface temperature, 50~620m/z of scanning range.
GC-MS analysis is carried out to oily camphor tree leaf essential oil, obtains oily camphor tree leaf essential oil total ion current figure, as shown in Figure 3.Its chemistry at
Divide and content is as shown in table 3, identifies 37 kinds of ingredients altogether from oily camphor tree leaf essential oil, account for the 94.023% of essential oil total amount, mainization
It is other to close species are as follows: 22 kinds of alkenes compounds, account for the 22.749% of essential oil total amount；13 kinds of alcohol compound, account for essential oil total amount
71.068%；1 kind of ester type compound, account for the 0.006% of essential oil total amount；1 kind of alkane derivative, account for essential oil total amount
0.200%.In these chemical components, 1,8- eudesmol, β-phellandrene, alpha-terpineol, 1R- π firpene, left-handed nopinene and
The compounds contents such as terpineol -4 are higher.
The chemical component and its content for the oily camphor tree leaf essential oil that 3 Simultaneous distillation of table extracts
3) Antibacterial Activity of the oily camphor tree leaf essential oil to fungi
The oily camphor tree leaf essential oil 1 that takes Simultaneous distillation to extract, 3,5,7mL and 25mL ZPD mixing inverted plate, are made oily
Camphor tree leaf essential oil concentration is the ZPD culture medium of 1: 25,3: 25,5: 25 and 7: 25 (volume ratios), and is compared with pure ZPD inverted plate.
Pathogen inoculated by hypha block is repeated 3 times to culture dish center, observes and take pictures after 25 DEG C of constant temperature incubation 4d respectively, is calculated antibacterial
Rate.The pathogen bacterium colony average diameter being wherein seeded on control plate is Do, the disease being seeded on oil-containing camphor tree leaf essential oil plate
Opportunistic pathogen bacterium colony average diameter is Dx, then
Bacteriostasis rate=(Do-Dx)/Do × 100%.
The bacteriostatic activity for the oily camphor tree leaf essential oil that 4 Simultaneous distillation of table extracts
By Fig. 4 and table 4 it is found that oily camphor tree leaf essential oil all has one to Fusarium oxysporum, Fusicoccum asculi and Phomopsis
Fixed inhibitory effect, and with the increase of different camphor tree leaf essential oil concentration, inhibitory effect is obvious.When concentration 7: 25 to disease fungus
Inhibitory effect is the most obvious, respectively reaches 80.17%, 90.85% and 61.25% to the bacteriostasis rate of 3 kinds of plant pathogenic fungis,
Oily camphor tree leaf essential oil is respectively Fusarium oxysporum and Phomopsis 1.12 and 1.48 times to the inhibiting rate of Fusicoccum asculi.
Claims (9)
1. a kind of oil camphor tree leaf essential oil bacteriostasis method, which comprises the following steps:
1) using oily camphor tree leaf as raw material, essence the extraction of oily camphor tree leaf essential oil: is extracted using steam distillation or Simultaneous distillation
Oil, the liquid distilled out, by water-oil separating, oil part is dry with anhydrous sodium sulfate, obtains oily camphor tree leaf essential oil；
2) inhibition plant pathogenic fungi is carried out as effective component using the oily camphor tree leaf essential oil that step 1) obtains.
2. oil camphor tree leaf essential oil bacteriostasis method according to claim 1, which is characterized in that in step 1), steam distillation
Ratio of water to material be 12.5: 1mLg-1。
3. oil camphor tree leaf essential oil bacteriostasis method according to claim 1, which is characterized in that in step 1), Simultaneous distillation-extraction
The ratio of water to material of method is 10: 1mLg-1, extractant material ratio is 2: 1mLg-1, extractant is anhydrous ether, extraction temperature 40
℃。
4. oil camphor tree leaf essential oil bacteriostasis method according to claim 1, which is characterized in that in step 2), plant pathogenic fungi
For Fusarium oxysporum, Fusicoccum asculi and Phomopsis.
5. oil camphor tree leaf essential oil bacteriostasis method according to claim 1, which is characterized in that in step 1), extracting power is
1200W, extraction time 65min.
6. oil camphor tree leaf essential oil bacteriostasis method according to claim 1, which is characterized in that antibacterial in use, oily in step 2)
The volume ratio of camphor tree leaf essential oil is 1-7: 25.
7. oil camphor tree leaf essential oil bacteriostasis method according to claim 6, which is characterized in that the volume ratio of oily camphor tree leaf essential oil is 7:
25,80%, 90% and 61% is not less than respectively to the bacteriostasis rate of Fusarium oxysporum, Fusicoccum asculi and Phomopsis.
8. oil camphor tree leaf essential oil bacteriostasis method according to claim 1, which is characterized in that in step 1), steam distillation
The oily camphor tree leaf essential oil of extraction, contained chemical component include: alkenes compounds, alcohol compound, ester type compound, aldehydes chemical combination
Object, alkane derivative.
9. oil camphor tree leaf essential oil bacteriostasis method according to claim 1, which is characterized in that in step 1), Simultaneous distillation-extraction
The oily camphor tree leaf essential oil that method is extracted, contained chemical component includes: alkenes compounds, alcohol compound, ester type compound, alkanes
Compound.
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| CN114052048A (en) * | 2021-10-25 | 2022-02-18 | 福建农林大学 | Cinnamomum camphora leaf extract and preparation method and application thereof |
| CN113875598A (en) * | 2021-11-19 | 2022-01-04 | 宜宾学院 | Method for inducing pinellia ternata callus and special culture medium thereof |
| CN114561246A (en) * | 2022-02-18 | 2022-05-31 | 东莞市农业科学研究中心 | Preparation method and bacteriostatic application of camphor tree essential oil |
| CN114561246B (en) * | 2022-02-18 | 2023-12-01 | 东莞市农业科学研究中心 | Preparation method of camphor tree essential oil and antibacterial application thereof |
| CN115530034A (en) * | 2022-09-23 | 2022-12-30 | 宜宾学院 | Method for green cultivation of pinellia ternata by utilizing cinnamomum camphora waste residues |
| CN115530034B (en) * | 2022-09-23 | 2024-02-02 | 宜宾学院 | Method for green cultivation of pinellia ternate by utilizing Cinnamomum camphora waste residues |
| CN117959359A (en) * | 2024-03-29 | 2024-05-03 | 江西瑞秀朗科技有限公司 | Camphor tree leaf extract for repairing skin barrier, and extraction method and application thereof |
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