CN115530034A - Method for green cultivation of pinellia ternata by utilizing cinnamomum camphora waste residues - Google Patents
Method for green cultivation of pinellia ternata by utilizing cinnamomum camphora waste residues Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/27—Pulp, e.g. bagasse
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
The invention relates to the technical field of pinellia ternate cultivation, and discloses a green cultivation method of pinellia ternate by utilizing cinnamomum camphora waste residues, aiming at the problem that tuber contamination diseases are easy to occur in artificial cultivation in half summer. According to the invention, the cinnamomum camphora waste residues are added into the cultivation soil, so that on one hand, the organic matter content in the soil can be improved, the antibacterial effect is exerted, the bacterial contamination diseases which are easy to occur in the cultivation process of the pinellia ternata are reduced, and the yield and the quality of the pinellia ternata are improved; on the other hand, environmental pollution and resource waste caused by direct incineration treatment of the cinnamomum camphora waste residue can be avoided.
Description
Technical Field
The invention relates to the technical field of pinellia ternate cultivation, and particularly relates to a green pinellia ternate cultivation method by utilizing cinnamomum camphora waste residues.
Background
Pinellia ternate is a dried tuber of Pinellia ternata (thunb.) Breit, a plant of Araceae, and is widely planted because it has the effects of drying dampness and resolving phlegm, calming the adverse-rising energy and preventing vomiting, relieving distension and fullness and dissipating stagnation, and the like. The resources of the wild pinellia ternata are gradually exhausted along with the excessive harvesting of the wild pinellia ternata and the deterioration of the wild environment, so that a mode of artificially cultivating the pinellia ternata is proposed to relieve the excessive consumption of the wild resources.
The pinellia ternate is mainly subjected to vegetative propagation, so that more and more pathogens are accumulated, the occurrence frequency of plant diseases and insect pests is higher and higher, and even the pinellia ternate is dead in delivery and dead in harvest; the frequent use of pesticides leads to the exceeding of pesticide residues and the gradual decline of the seed quality, so that the pinellia ternata cultivation conditions are usually required to be subjected to insect pest prevention treatment. For example, patent publication No. CN110226481a discloses a simplified cultivation method for pinellia ternate with disease and pest prevention and easy harvesting, which is characterized in that artemisia argyi straw is laid at the bottom of a farmland tank, and the problem of high incidence of diseases and pests of pinellia ternate is relieved by virtue of the antibacterial property of the artemisia argyi straw. However, the artemisia argyi straw has a weak effect of preventing diseases and pests in pinellia ternate cultivation, and the quality of the cultivated pinellia ternate is poor.
Therefore, it is particularly urgent to find a cultivation method which can not only ensure the cultivation quality of pinellia ternata, but also reduce the incidence rate of plant diseases and insect pests.
Disclosure of Invention
The technical problems to be solved by the invention are as follows:
at present, the quality of the pinellia ternata medicinal material is difficult to meet the requirements of pharmacopoeia due to the fact that cultivation soil and environment are affected by high toxicity and high pesticide residue when artificial cultivation is adopted for half summer; meanwhile, in the process of cultivating and planting pinellia ternata, tuber parts and the like are extremely susceptible to root rot and damping off due to the vegetative propagation growth mode, and pinellia ternata has strong tolerance to common bacteriostatic agents, so that the disease control is difficult, the control cost is high, the yield of the pinellia ternata is further seriously reduced, and the quality of the cultivated pinellia ternata is low.
The technical scheme adopted by the invention is as follows:
the invention provides a method for green cultivation of pinellia ternate by utilizing cinnamomum camphora waste residues.
Preferably, in the cultivation container, the cinnamomum camphora waste residues are laid at the bottom of the cultivation container, and the surfaces of the cinnamomum camphora waste residues are covered with soil.
Preferably, the method for green cultivation of pinellia ternata by using cinnamomum camphora waste residues comprises the following steps:
(1) Taking waste residues left after distilling the cinnamomum longepaniculatum leaves, and finely crushing to obtain cinnamomum longepaniculatum waste residues;
(2) Spreading the cinnamomum camphora waste residue at the bottom of the cultivation container, covering soil on the surface of the cinnamomum camphora waste residue, and planting pinellia ternata for cultivating seedlings.
Preferably, the paving thickness of the cinnamomum camphora waste residue is 1-3cm; further, the paving thickness of the cinnamomum camphora waste residue is 1cm or 2cm or 3cm.
Preferably, the covering thickness of the soil is 2-5cm; further, the covering thickness of the soil is 5cm.
The beneficial effects of the invention are as follows:
by adding the cinnamomum camphora waste residues into the cultivation soil, on one hand, the organic matter content in the soil can be improved, the antibacterial effect is exerted, the bacterial contamination diseases which are easy to occur in the cultivation process of the pinellia ternata are reduced, and the yield and the quality of the pinellia ternata are improved; on the other hand, the cinnamomum longepaniculatum essential oil can be only used for inducing a culture medium for callus of pinellia ternata, but callus formed by pinellia ternata is located in the culture medium, required nutrient substances are different from nutrient substances required for cultivation in soil, the cinnamomum longepaniculatum waste residue obtained after cinnamomum longepaniculatum essential oil is distilled from cinnamomum longepaniculatum leaves is different from cinnamomum longepaniculatum essential oil in content and action effect, the cinnamomum longepaniculatum waste residue is usually directly subjected to waste treatment or is directly combusted as a dye, so that environmental pollution and resource waste are caused, and the cinnamomum longepaniculatum waste residue is used as a soil additive for cultivating pinellia ternata, so that the problems can be effectively avoided.
Drawings
FIG. 1 shows the effect of Cinnamomum camphora added waste residue on the emergence stage of pinellia ternata in test example 2;
FIG. 2 shows the effect of Cinnamomum longepaniculatum added waste residues on the content of semi-summer proline (T1-T4) in test example 2;
FIG. 3 is a graph showing the effect of added Cinnamomum longepaniculatum waste residues on the content of semi-summer proline (T5-T8) in test example 2;
FIG. 4 shows the effect of the added Cinnamomum camphora waste residues on the content of soluble proteins in semi-summer (T1-T4) in test example 2;
FIG. 5 shows the effect of Cinnamomum camphora added waste residue on the content of soluble protein in semi-summer (T5-T8) in test example 2
FIG. 6 is a graph showing the effect of Cinnamomum camphora added waste residue on POD activity of pinellia ternate in test example 2;
FIG. 7 shows the effect of the added Cinnamomum camphora waste residues on the SOD activity (T1-T4) of pinellia ternata in test example 2;
FIG. 8 is a graph showing the effect of the added Cinnamomum longepaniculatum waste residue on the SOD activity (T5-T8) of pinellia ternata in test example 2.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The invention provides a method for green cultivation of pinellia ternate by utilizing cinnamomum camphora waste residues, which comprises the following steps:
(1) Taking waste residues left after distilling the cinnamomum longepaniculatum leaves, and finely crushing to obtain cinnamomum longepaniculatum waste residues;
(2) Paving the cinnamomum camphora waste residues at the bottom of the cultivation container, wherein the paving thickness of the cinnamomum camphora waste residues is 1-3cm; covering soil on the surface of the cinnamomum longepaniculatum waste residue, wherein the coverage thickness of the soil is 2-5cm, and planting pinellia ternata for seedling culture.
Wherein, after the cultivation according to the scheme, management is carried out according to conventional water and fertilizer treatment and the like. Specifically, according to the actual drought condition of soil, supplementing water reasonably in time; and weeding is carried out by manually pulling out the weeds regularly. In the seedling emergence stage, observing and counting the growth conditions of the seedlings every day; after the seedlings emerge stably, observing the occurrence condition of plant diseases and insect pests every other week, and measuring the physiological indexes every other two weeks.
Example 1
Taking waste residues left after distilling the cinnamomum longepaniculatum leaves, and finely crushing to obtain cinnamomum longepaniculatum waste residues; paving the cinnamomum camphora waste residues at the bottom of the cultivation container, wherein the paving thickness of the cinnamomum camphora waste residues is 2cm; and covering soil on the surface of the cinnamomum longepaniculatum waste residue, wherein the covering thickness of the soil is 5cm, and planting pinellia ternata for seedling culture. After being finely crushed, the cinnamomum longepaniculatum waste residue is mixed with the cultivation soil according to the volume ratio of about 1:2, the pinellia ternate cultivation can prolong the growing period of the pinellia ternate, so that the leaves are black and green, the plants are strong and strong, the growth is good, the occurrence degree of plant diseases and insect pests is low, the propagation coefficient and the yield are also obviously improved, and the occurrence of plant diseases and insect pests is reduced.
Test example 1
Subject of the test: influence of directly adding cinnamomum camphora waste residue on growth of pinellia ternata
TABLE 1 influence of Cinnamomum camphora waste residue on the growth of pinellia ternata
TABLE 2 influence of Cinnamomum camphora waste residue on pinellia ternata seedling culture
As can be seen from tables 1 and 2, the direct addition of cinnamomum camphora residues in different ways has significantly different effects on the growth of pinellia ternata. The test example 1 has the best effect, and each index is obviously improved compared with CK. While the test examples 2 and 3 are poor in effect and even far lower than CK, grubs are found in the test example 2, which causes the number of tuber and bulbil of pinellia ternata to be greatly reduced; in test example 3, the growth of the mushroom fungus also adversely affected the growth of pinellia ternate, resulting in a significantly lower production index than CK.
Test example 2
Subject of the test: influence of the added finely-crushed cinnamomum camphora waste residue on the growth of pinellia ternata
Adopt potted plant contrast test method, set up 8 and handle the group:
| t1 (slag: soil = 1:1) | T5 (slag: soil = 1:1) |
| T2 (slag: soil = 1:2) | T6 (slag: soil = 1:2) |
| T3 (slag: soil = 1:3) | T7 (slag: soil = 1:3) |
| T4 (slag: soil = 1:4) | T8 (slag: soil = 1:4) |
Wherein, the waste residues added in the four treatment groups of T1-T4 are the cinnamomum camphora waste residues after the oil removal in the second year; and adding the cinnamomum camphora waste residues which are just deoiled into the four treatment groups of T5-T8. The potted plants are placed at the same position and under proper conditions, the pinellia ternate seedballs with complete disease-free diameters of 1cm-1.5cm are selected for planting, 20 seedballs are planted in each pot, the cinnamomum camphora waste residues after oil removal and cinnamomum camphora waste residues after oil removal for one year are ground into powder and mixed with soil according to different volume proportions, and the pinellia ternate seedballs are planted by taking the cinnamomum camphora waste residue soil which is not added as a reference.
The growth of pinellia ternata was observed with direct overlay as control. 14 pinellia ternata aseptic seedlings are planted in each pot, and the process is repeated three times. After 5 months of growth, growth was as follows:
TABLE 3 Effect of finely divided Cinnamomum longepaniculatum waste residues on growth in semi-summer for 5 months
| Adding amount of | Growth of pinellia Tuber |
| Direct covering | Pinellia tuber has poor growth vigor, dry leaf apex, short growth period and early seedling falling time |
| T1 | Good growth vigor and tender green leaves |
| T2 | The best growth vigor, flourishing, black and green leaves, robust |
| T3 | Better growth, weaker than treatment B and stronger than treatment A |
| T4 | Weak growth vigor but stronger than CK treatment |
The results show that the direct coverage effect is the worst, and the inhibition effect is generated on the normal growth of the pinellia ternata, while the addition of different waste residue powders has different effects on the growth of the pinellia ternata. The effect of T2 is best, the addition amount is larger, the growth promoting effect on the pinellia ternata is obvious, and the growth promoting effect is gradually reduced along with the reduction of the addition amount. The growth vigor of T4 is basically consistent with that of the added waste residues, and the action mechanism is reduced. Through continuous observation, the T2 and T3 seedling pouring time is delayed for about 15 days compared with other treatment groups.
After seedling pouring, the quantity of semi-summer bulbil buds, the quality of tubers and the biomass are measured, and the results are shown in the following table:
TABLE 4 growth of Fall-sprout Kanzhiza plants
As can be seen from Table 4, T2 was found to have the highest biomass and the highest yield, as well as the highest propagation coefficient for the bead buds and tubers compared to the other treatment groups.
(1) The influence of camphor wood waste residue on the emergence stage of pinellia ternata:
taking 5 leafstalks as the seedling emergence stage of the pinellia ternata, wherein the seedling emergence stage is respectively as follows: CK: 25 months and 3 days, T1: day 25, 3 month, T2: 25 days 3 month, T3:3 month, 27 days, T4: 25 days 3 month, T5: 25 days 3 month, T6: 29 days 3 and T7: 28 days 3 month, and 31 days 3 month T8; therefore, the cinnamomum longepaniculatum waste residues T6, T7 and T8 which are just deoiled have obvious inhibition effect on seedling emergence in semi-summer.
As can be seen from the attached figure 1, by 10 days in 4 months, the numbers of CK, T1, T2, T3 and T4 petioles are optimistic, while the numbers of T5, T6, T7 and T8 petioles do not reach 20, the germination rate is low and are respectively T5:65%, T6:60%, T7:75%, T8:70 percent. Therefore, the cinnamomum camphora waste residue after deoiling for one year has the effect of promoting growth in the seedling emergence stage of the pinellia ternate, and the promoting effect is weakened along with the reduction of the addition concentration of the waste residue; the camphor waste residue which is just removed has obvious inhibition effect on growth.
(2) The influence of the added cinnamomum camphora waste residue on the height of the plants in the semi-summer:
by integrating plant height data of three time nodes of day 27 at 4 months, day 11 at 5 months and day 25 at 5 months, as shown in Table 5 below, the T2 treatment group was the highest, while the T1 and T4 treatment groups were slightly shorter than CK; the T6 has the shortest plant height, and the plant heights are sequentially from high to low: t2, CK, T1, T4, T8, T7, T3, T5, T6.
TABLE 5 influence of Cinnamomum longepaniculatum waste residues on height of semi-summer plants
(3) Influence of Cinnamomum camphora added waste residue on half Xia Shekuan leaf length:
the approximate leaf area of pinellia ternata was calculated from the leaf width x leaf length by combining the leaf width and leaf length data measured at 22 days 4-month, 6 days 5-month, 25 days 5-month, 3 days 6-month and 17 days 6-month as shown in table 6 below. Among them, the area of the T1 leaf is the largest, and the second is T2, and the areas of the two leaves are larger. T4, T8 are not much different than CK, while the three processing leaf areas T5, T6 and T7 are much smaller than CK.
TABLE 6 influence of Cinnamomum camphora waste residue on the growth of leaf width of semi-summer
(4) Adding cinnamomum camphora waste residues to influence the growth form and diseases of pinellia ternata:
the abnormal leaves (possibly diseases, but no insects exist) are the number of leaves, the stem soft rot disease refers to the soft rot condition of the stems after the pinellia ternata is poured into the seedlings, and no obvious rot is seen before the seedlings are poured. The T7 treatment group had the most malformed leaves, T6 and T8 malformed leaves were relatively more, and the other treatment groups had fewer malformed leaves relative to the CK placebo group; all treatment groups had the effect of inhibiting soft stalk rot, relative to the blank control group CK, with T3, T6 being the best, as shown in table 2.
By 25 days 6 months after 2022 years, as shown in Table 3, the seedling stage of T5 treatment is the earliest, the seedling stage of T4, T6 and T7 is relatively earlier, and the pinellia ternata is transplanted in advance by four treatment groups of T4, T5, T6 and T7, wherein the effect of T5 on transplanting pinellia ternata in advance is most obvious. By this day (27/6/2022), the seedlings of CK, T5, T6, T7 and T8 are not completely poured, T2 is firstly completely poured, the time for completely pouring the seedlings is 6 months and 10 days, and then T1, T3 and T4 are carried out, and the time for completely pouring the seedlings is 6 months and 19 days.
TABLE 7 influence of Cinnamomum camphora waste residues on growth morphology and disease of pinellia ternata
(5) Adding cinnamomum camphora waste residues to influence the chlorophyll content of pinellia ternata:
as shown in Table 7 below, the absence of T2 and T4 data in the chlorophyll content of pinellia tuber at day 2/6 is mainly due to the absence of measurable data when the pinellia tuber has fallen (no leaf was measured, but the subsequent pinellia tuber also emerged). The overall content of CK chlorophyll is relatively high, but the content of chlorophyll gradually decreases in the whole growth process; the T2 content is still slightly less than the CK content; the chlorophyll content of T1 and T3 is gradually reduced, and the chlorophyll content of T5, T6, T7 and T8 is increased and then reduced; the T5 and T6 chlorophyll content was less compared to the other treatment groups and CK. The chlorophyll content of the cinnamomum longepaniculatum waste residue after oil removal for one year is obviously higher than that of the cinnamomum longepaniculatum waste residue after oil removal.
TABLE 7 influence of Cinnamomum camphora waste residues on chlorophyll content of pinellia ternata
The physiological effect of adding cinnamomum camphora on resistance to summer is as follows:
(6) The influence of the cinnamomum camphora waste residue on the content of semi-summer proline is added:
as shown in fig. 3, T2 has the highest proline content measured at 3 days 6 months, and shows a trend of increasing after decreasing; the whole level of proline content is higher in 6 months and 3 days, wherein the T2 content is increased suddenly; except T4 and T7, the proline content of other treatment groups shows a trend of being less and then increasing, and the overall content of T3 is high.
(7) The influence of the cinnamomum camphora waste residue on the soluble protein content of the pinellia ternata:
as shown in FIG. 4, the soluble protein content of each treatment group was not very different at each time period. Wherein the lowest content of soluble protein in the T3 treated group is 5 months and 20 days, the lowest content of soluble protein in the T4 treated group is the highest, and the content is determined for T5 times; CK. The content of the soluble protein of T1 is gradually increased in the growth process, and the content of the soluble protein of T3 is obviously reduced and then increased; the remaining treatment groups are all incremented or decremented by small increments.
(8) The influence of the cinnamomum camphora added waste residue on the activity of the pinellia ternate POD:
as shown in fig. 5, the activity of seven groups of POD (CK, T1, T2, T3, T5, T7 and T8) has gradually enhanced tendency, but the activity of other PODs except T7 is finally reduced; t4 POD activity shows a trend of decreasing first and then increasing; t6 POD activity showed a trend of increasing first, then decreasing last. Compared with CK, the POD activity of other treatment groups except T1 is lower than that of CK group POD, wherein the POD activity of four groups of cinnamomum camphora residues T1-T4 after one year of deoiling is obviously stronger than that of cinnamomum camphora residues after the first year of deoiling is compared with that of cinnamomum camphora residues after the last year of deoiling.
(9) The influence of camphor wood waste residue on the activity of pinellia ternate SOD is added:
SOD activity was calculated by measuring fresh weight, volume and Abs values at different absorbances of the samples, as shown in FIG. 6, and all treatment groups had the highest SOD activity at 21 days in 5 months, with some decrease in activity at 4 days in 6 months, and increase in activity at 18 days in 6 months and T8 days. The average value of the last summary of T7 and T8 is low, and may be that the average value is reduced for five times of measurement times, and the average value of the last summary of T4 and T5 is high, and may be that the measurement times are 3 times, and the result may have an error.
Through testing and determining the growth conditions and the like of the pinellia ternate cultivated by adding the cinnamomum camphora waste residues into the soil, the method can obtain the following steps: the growth states of the pinellia ternata treated differently are obviously different, and waste residue after one year of oil removal is more beneficial to growth of the pinellia ternata in half summer, which is mainly reflected in seedling emergence condition, plant height, leaf area and the like; although the seedling pouring period of the waste pinellia tuber which is one year after oil removal is relatively later than that of the waste pinellia tuber which is just subjected to oil removal, the seedling pouring time is opposite; in the physiological aspect of resistance, in the later stage of a test, the content of T2 proline is increased suddenly, the stress resistance is strong, the POD activity of four groups T1-T4 is obviously higher than that of four groups T5-T6, the SOD activity of T4 is strongest, and the stress resistance is strong; the soluble protein indirectly reflects the content of enzyme, the content of T4 soluble protein is the highest, and the content of T3 is the lowest.
Therefore, the cinnamomum camphora waste residue after oil removal for one year has better growth on pinellia ternate, the seedling falling period is delayed, the complete seedling falling time is advanced, and the stress resistance after early oil removal for one year is better; wherein the stress resistance of T4 is relatively stronger, but the growth vigor is not as good as that of T1 and T3, and the growing period of the just deoiled cinnamomum longepaniculatum waste residue is longer. The conclusion of a particular test also needs to be concluded from the final yield.
Based on test examples, according to the invention, the cinnamomum camphora waste residues are added into the cultivation soil, and the cinnamomum camphora waste residues can be found to have a promoting effect on semi-summer cultivation by integrating various growth performances: the fermentation process in the later period can be reduced, bacteriostatic substances and rich carbon and nitrogen elements in the cinnamomum camphora waste residues are fully utilized, the pest and disease damage in the pinellia ternate cultivation process is reduced, the use of pesticides such as bacteriostatic agents is reduced, meanwhile, organic matters in soil are increased, the physical and chemical properties of the soil are improved, and the batch planting of the pinellia ternate is realized; and the cinnamomum camphora waste residue resource is fully utilized, and the environmental pollution caused by the cinnamomum camphora waste residue incineration treatment is reduced.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A method for green cultivation of pinellia ternate by utilizing cinnamomum camphora waste residues is characterized by adding cinnamomum camphora waste residues into soil for cultivating pinellia ternate, wherein the cinnamomum camphora waste residues are the waste residues left after cinnamomum camphora leaves are distilled and extracted to obtain cinnamomum camphora essential oil.
2. The method for green cultivation of pinellia ternate by utilizing cinnamomum camphora residues according to claim 1, wherein the cinnamomum camphora residues are laid at the bottom of the cultivation container in the cultivation container, and the surface of the cinnamomum camphora residues is covered with soil.
3. The method for green cultivation of pinellia ternate by utilizing cinnamomum camphora residues as claimed in claim 2, comprising the following steps:
(1) Taking waste residues left after distilling the cinnamomum longepaniculatum leaves, and finely crushing to obtain cinnamomum longepaniculatum waste residues;
(2) Spreading the cinnamomum camphora waste residue at the bottom of the cultivation container, covering soil on the surface of the cinnamomum camphora waste residue, and planting pinellia ternata for cultivating seedlings.
4. The method for green cultivation of pinellia ternate with cinnamomum camphora linn waste residues according to claim 3, wherein the thickness of the cinnamomum camphora linn waste residues is 1-3cm.
5. The method for green cultivation of pinellia ternate by utilizing cinnamomum camphora waste residues according to claim 4, wherein the paving thickness of the cinnamomum camphora waste residues is 1cm, or 2cm, or 3cm.
6. The method for green cultivation of pinellia ternate utilizing cinnamomum camphora residues according to claim 3, wherein the coverage thickness of the soil is 2-5cm.
7. The method for green cultivation of pinellia ternate utilizing cinnamomum camphora residues according to claim 6, wherein the coverage thickness of the soil is 5cm.
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