CN106490032A - A kind of biological pesticide of preventing and treating rice pest and preparation method thereof - Google Patents
A kind of biological pesticide of preventing and treating rice pest and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention provides a kind of biological pesticide of preventing and treating rice pest, belongs to technical field of pesticide.A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：5 ~ 20 parts of 0.1 ~ 0.5 part of AVM, 0.5 ~ 3 part of bamboo vinegar, 1 ~ 5 part of chitin and Chinese medicine composition, the Chinese medicine composition is made up of the raw material of following weight parts：Aglaia odorata is spent 10 ~ 30 parts, 10 ~ 30 parts of the coptis, 5 ~ 20 parts of needle juniper, white drenches 5 ~ 20 parts of grass, 5 ~ 20 parts of cuckoo meat, 5 ~ 15 parts of Inula britannica chinensis, 3 ~ 12 parts of vernonia anthelmintica, 3 ~ 12 parts of elm bark, 3 ~ 12 parts of calamus.The present invention can play a part of Synergistic by AVM, bamboo vinegar, chitin and Chinese medicine composition through science mixture, so that the biological pesticide antibacterial killing ability for obtaining is significantly increased, expanding prevention scope, reduces poisoning incidence, not only efficient but also saving of work and time.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of biological pesticide of preventing and treating rice pest and its preparation side
Method.
Background technology
China is that kind rice is also to produce the most country of paddy, paddy rice average per unit area yield in various cereal crops earliest in the world
Highest.On the south the Rice Cropping region of China based on side, present in recent years and progressively produce to the middle and lower reach of Yangtze River and Heilungkiang paddy rice
The trend that the dominant areas such as area are concentrated.Paddy rice is subject to pest and disease damage invasion and attack in process of production, and pest species are more, therefore agriculture
The amount of application of medicine remains high.Data show, the amount of application of Rice Production process Pesticides about accounts for Chinese agricultural chemicals and always disappears
15% of the amount of expense or so.
Paddy rice is easily endangered by multiple pest and disease damages during planting, and the species survey of pest and disease damage is more, distribution is wide, propagation
Fast and damage losses are serious, and Major Diseases include：Rice sheath blight disease, rice blast, bakanae disease and stripe virus disease etc.；Mainly
Insect pest includes：White backed planthopper and brown paddy plant hopper, the snout moth's larva of rice and rice leaf roller etc..For a long time, people are mainly using chemical agriculture
Medicine controls diseases and pests of agronomic crop.Due to the long-term a large amount of and Reusability of chemical pesticide, dirt is generated to soil, water body and air
Dye, make agricultural byproducts Pesticide Residues increase, also directly compromise the mankind health and existence and insect to chemical pesticide
The resistance to the action of a drug is also being improved year by year, while the cost accounting more and more higher chemical pesticide control insect of crops, has also killed day
Enemy and other beneficial organisms, destroy the ecological balance.Progress, expanding economy, Environmental security and food with science and technology and society
Product become safely the key subjects that the whole mankind pays close attention to jointly.High poison, the production of high residue chemical pesticide and use is limited, is pushed greatly
Extensively using safety, low toxicity, the biological pesticide of environment-friendly type, a developing direction is had become.Therefore, the preventing and treating to rice pest
It is also proposed higher requirement.
And biological pesticide be directly using biogenic bioactivator or biological living mycelium, half armful of crystal,
Insect viruses etc. are given birth to including microbial pesticide, farm antibiotics, botanical pesticide, biochemical pesticides, transgenosis as agricultural chemicals
Thing agricultural chemicals and natural enemy biopesticide.Compared with chemical pesticide, biological pesticide has the advantage that：1. the toxicity of biological pesticide is usual
Lower than chemical pesticide, to person poultry safety, great majority have no toxic side effect；2. selective strong, specially to harmful organism target, do not kill evil
Worm natural enemy, is not for non-target organism, is " protection type agricultural chemicals "；3. low-residual, efficiently.Chemical pesticide can be avoided on the whole
The problem of environmental pollution for bringing；4. the resistance to the action of a drug is not likely to produce, long service life, dispenser cost are low, is " benefit agricultural chemicals "；⑤
As a constituent of integrated pest control, the usage amount of chemical pesticide can be greatly reduced, and does not affect crop yield.
China's living resources are enriched, and have the Biological Genetic Resources in the whole world, provide advantage for developing biological pesticide.
Liu Zhenhai etc.（Liu Zhenhai, Zhang Haiqing, seize by force beautiful Na. and biogenic pesticide Ningnanmycin is imitated to the preventing and treating of rice seedling blight
Really [J]. crop investigations, 2009,23 (4):265-268）Have studied biogenic pesticide Ningnanmycin to prevent rice seedling blight
Control effect.Using indoor Mycelial growth method and growth rate method, with dominant bacteria point spore reaping hook of the Ningnanmycin to rice seedling blight
Bacterium, Rhizoctonia solani Kuhn and pythium spp have carried out inhibiting rate and toxicity test, and determine Ningnanmycin by field plot trial
Prevention effect and the impact of seedling quality to rice seedling blight.As a result show, 200 times of Ningnanmycin is to 3 kinds of germ mycelia
Inhibiting rate highest, after inoculation, the average inhibition of the 7th d is respectively 74.9%, 65.9% and 69.6%；EC50For 34.49 ~
111.39 mg/L；As Ningnanmycin consumption increases, damping-off preventive effect is improved, with 12.5 ~ 25 g/m2Effect is more excellent；Ningnan
Mycin process slightly has facilitation to Seedling Quality in Rice.The patent of Application No. 201510303796.2 discloses a kind of plant
Source pesticide, the botanical pesticide are shown in by Chinese herbal medicine Radix Glycyrrhizae, phellodendron bark, China tree, cockscomb, radix ranunculi ternati, Flos Albiziae, butterflybush flower, stone
Wear, Lu Bazi, Golden Larch Bark are produced.Through field test, to the downy mildew of the crops such as melon fruits and vegetables, paddy rice, sclerotiniose, powdery mildew,
The prevention effect of multiple fungal diseases such as gray mold, anthracnose, epidemic disease, balck shank, banded sclerotial blight is notable.Technology disclosed above shows
Show, whether single or combination biological pesticide achieves certain prevention effect to rice pest.Meanwhile, biological pesticide exists
While constantly development, there is also some distinct issues and difficulty annoyings which and advances, for example speed knocked down by biological pesticide
Degree is slow, and the term of validity is shorter, and relative pesticidal spectrum is narrower, not strong to pest population controling power in a short time, it is impossible to rapid reduction insect worm
Mouth density etc..
Content of the invention
In view of this, the technical problem to be solved is to provide a kind of biological pesticide of preventing and treating rice pest,
Antibacterial insecticidal effect is stable, strong drug action, knocks down speed to insect soon, and control time is long.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM 0.1 ~ 0.5
5 ~ 20 parts of part, 0.5 ~ 3 part of bamboo vinegar, 1 ~ 5 part of chitin and Chinese medicine composition, original of the Chinese medicine composition by following weight parts
Material is made：Aglaia odorata spend 10 ~ 30 parts, 10 ~ 30 parts of the coptis, 5 ~ 20 parts of needle juniper, white 5 ~ 20 parts of the grass, 5 ~ 20 parts of cuckoo meat, sheathed of drenching
Spend 5 ~ 15 parts, 3 ~ 12 parts of vernonia anthelmintica, 3 ~ 12 parts of elm bark, 3 ~ 12 parts of calamus.
Preferably, the Chinese medicine composition is made up of the raw material of following weight parts：Aglaia odorata spends 20 ~ 25 parts, the coptis 15 ~ 20
Part, 8 ~ 12 parts of needle juniper, white drench 8 ~ 12 parts of grass, 8 ~ 12 parts of cuckoo meat, 8 ~ 12 parts of Inula britannica chinensis, 6 ~ 8 parts of vernonia anthelmintica, elm bark 6 ~
8 parts, 6 ~ 8 parts of calamus.
Preferably, the Chinese medicine composition is made up of the raw material of following weight parts：Aglaia odorata spend 25 parts, 18 parts of the coptis, Du
10 parts of pine, 10 parts of white pouring grass, 10 parts of cuckoo meat, 10 parts of Inula britannica chinensis, 7 parts of vernonia anthelmintica, 8 parts of elm bark, 8 parts of calamus.
Preferably, the biological pesticide of the preventing and treating rice pest, is prepared from by the raw material of following weight portion：Avermectin
10 ~ 15 parts of 0.2 ~ 0.3 part of element, 1 ~ 2 part of bamboo vinegar, 3 ~ 4 parts of chitin and Chinese medicine composition.
Preferably, the Chinese medicine composition is obtained by following preparation methods, and the preparation method is comprised the following steps：
1）The Aglaia odorata flower of dry above-mentioned weight, the coptis, Bai Lincao, cuckoo meat, vernonia anthelmintica, elm are weighed respectively
Bark and calamus, mixing, are crushed after freezing under nitrogen protection, obtain comminuting matter I；Weigh respectively dry above-mentioned heavy
The needle juniper and Inula britannica chinensis of amount part proportioning, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 ~ 3 times of its weight, it is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；
The filtrate I and filtrate II are merged and obtains filtrate, relative density is 1.12 ~ 1.15 when the filtrate is concentrated into 75 DEG C
Medicinal extract, in the medicinal extract add volume fraction for 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, keep 18 ~ 24 hours,
Then filter, obtain filtrate III and sediment, relative density is 1.20 ~ 1.25 when the filtrate III is evaporated to 55 DEG C
Lotion；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with organic solvent, sub-cloud solution, vacuum revolving instrument steams organic solvent, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
Preferably, the w/v 1g of the medicinal extract and the ethanol：10~20ml.
Preferably, the organic solvent is dichloromethane or chloroform.
A kind of preparation method of the biological pesticide of preventing and treating rice pest, comprises the following steps：
1）Bamboo vinegar 0.5 ~ 3 part, chitin 2 ~ 6 part and the Chinese medicine composition 5 ~ 20 part of weight portion are weighed respectively, then in temperature
30 ~ 40 DEG C, carry out mixing 10 ~ 15 minutes under conditions of 2000 ~ 2500rpm of rotating speed, stand 15 ~ 20 minutes, mixed
Thing；
2）0.1 ~ 0.5 part of AVM for weighing weight portion is added in the mixture, 3000 ~ 3200rpm of rotating speed stirrings 30 ~ 40
Minute, stand, obtain final product.
Compared with prior art, its advantage is as follows for the present invention：
1）The invention provides a kind of biological pesticide of preventing and treating rice pest, applicant has deeply research to Rice Cropping, abiding by
Follow, find AVM, bamboo vinegar, crust
Element and Chinese medicine composition can play a part of Synergistic through science mixture so that the biological pesticide antibacterial killing ability for obtaining
Significantly increase, expanding prevention scope, reduce poisoning incidence, not only efficient but also saving of work and time.
2）Composite pesticide of the present invention can be used for the multiple pest and disease damages for preventing and treating the appearance of paddy growth cycle, mainly include paddy rice line
Rot, rice blast, stripe virus disease, white backed planthopper, the snout moth's larva of rice and rice leaf roller, field test find rice banded sclerotial blight
The incidence of disease of disease have dropped 52.1%, and disease-resistant rate is 95.7 ~ 100%；The incidence of disease of rice blast have dropped 71.6%, disease-resistant rate
For 96.5 ~ 100%；The incidence of disease of stripe disease have dropped 76.2%, and disease-resistant rate is 97.4 ~ 100%；Whitebacked Planthopper, water
The killing rate of rice borer and rice leaf roller can reach 100%.Therefore, biological pesticide of the present invention is applied to significantly improve water
The disease-resisting, insecticidal ability of rice, simultaneous effect of controlling are projected, and efficacy stability is with a wide range of applications.
3）The present invention is raw materials used to be natural, environmental protection raw material.The mode of action of AVM with stomach poison function is mainly
Main, have action of contace poison concurrently, to disturb the physiologically active of insect nervous system, be initially in paralysis symptom, subsequently after insect food medicine
Activity and trophic behaviour are slow dead up to stopping.Bamboo vinegar be made charcoal with bamboo wood during, collect bamboo wood in high temperature point
The gas produced in solution, and this gas is cooled down liquid substance at normal temperatures that obtain.Chitin is a kind of high score of line style
Sub- polysaccharide, with antibacterial insecticidal activity, preferred molecular weight is 3000 ~ 4000 chitin, and the antibacterial killing ability after compounding is more
By force；Chinese medicine composition is formed by plurality of raw materials medicine scientific compatibility, nontoxic, can not only antibacterial desinsection, and be applied to paddy rice tool
There is promotion to increase, improve paddy quality and other effects.After above-mentioned raw materials are compounded by science, mutual help, to the whole life of paddy rice
There is the pest and disease damage that long period occurs obvious prevention effect, good absorbing effect not to have any injury to non-target object.
Specific embodiment
For a better understanding of the present invention, present disclosure, but this are further fairly set out with reference to embodiment
Bright protection content is not limited solely to the following examples.In the following description, give a large amount of concrete details so as to
More thorough understanding of the invention is provided.It will be apparent, however, to one skilled in the art that the present invention can be with
It is carried out without the need for one or more of these details.
Embodiment 1：A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM hereinafter
18 parts of 0.2 part of rhzomorph, 1.5 parts of bamboo vinegar, 3.5 parts of chitin and Chinese medicine composition, the Chinese medicine composition is by following weight parts
Raw material make：Aglaia odorata is spent 25 parts, 18 parts of the coptis, 10 parts of needle juniper, white drench 10 parts of grass, 10 parts of cuckoo meat, 10 parts of Inula britannica chinensis, drives
7 parts of worm ringdove chrysanthemum, 8 parts of elm bark, 8 parts of calamus.
In the present invention, Aglaia odorata is spent, another name：Patrol sieve flower, Milan flower, tree orchid, gas delicate fragrance, include multiple volatile oil into
Point.The coptis, another name：Taste connects, river connects, Rhizoma Coptidis, is Ranunculaceae, Coptis herbaceos perennial.Needle juniper, is cupressaceae plant Du
The branches and leaves and cone of pine, include multiple volatile oil components.White pouring grass, is the herb of contrayerva Herba Pileae longicaulis.Cuckoo meat,
Another name：Red cuckoo, quick-fried cane cuckoo, can kill bamboo locust, oryza chinensis, mythimna separata, lice, mollusk etc., can also prevent and treat the late blight of potato.Rotation
Flower is covered, also known as：Jin Fohua, inula etc., belong to composite family, Inula perennial herb.Vernonia anthelmintica, is feverfew drive
The fruit of worm ringdove chrysanthemum, containing multiple volatile oil components.Elm bark, is that drying for Ulmaceae elm Ulmus pumila L. is set
Skin or root skin, can be used for urinary obstruction, stranguria with turbid discharge, oedema, ulcer carbuncle on the back, erysipelas, mange.Calamus, can for ward off dirty have one's ideas straightened out, a surname gas by
Phlegm, removing toxic substances, desinsection.
The preparation method of the biological pesticide of the present embodiment preventing and treating rice pest, comprises the following steps：
1）Bamboo vinegar, chitin and the Chinese medicine composition of above-mentioned weight portion are weighed respectively, then in 35 DEG C of temperature, rotating speed
Carry out mixing 15 minutes under conditions of 2500rpm, stand 20 minutes, obtain mixture；
2）The AVM for weighing above-mentioned weight portion is added in the mixture, and rotating speed 3000rpm is stirred 35 minutes, is stood, i.e.,
?.
Chinese medicine composition described in the present embodiment is obtained by following preparation methods：
1）Dry above-mentioned Aglaia odorata flower, the coptis, Bai Lincao, cuckoo meat, vernonia anthelmintica, elm bark and calamus are weighed respectively,
Mixing, is crushed after freezing under nitrogen protection, obtains comminuting matter I；Dry above-mentioned needle juniper and Inula britannica chinensis are weighed respectively,
Mixing, crushes, and obtains comminuting matter II；
2）The water of 5 times of its weight is added in the comminuting matter I, 50 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 times of its weight, 55 DEG C are heated to, are kept
35 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.13 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
28 DEG C, kept for 24 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.23 lotion；
3）The water of its 4 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 38 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
The medicinal extract is 1g with the w/v of the ethanol：16ml.
The using method of biological pesticide of the preventing and treating rice pest is：Add the biological pesticide weight 300 again with
On water, be thoroughly mixed, make spraying agent.
Embodiment 2：A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM hereinafter
20 parts of 0.3 part of rhzomorph, 1 part of bamboo vinegar, 4 parts of chitin and Chinese medicine composition, original of the Chinese medicine composition by following weight parts
Material is made：Aglaia odorata is spent 25 parts, 15 parts of the coptis, 12 parts of needle juniper, white drenches 8 parts of grass, 12 parts of cuckoo meat, 12 parts of Inula britannica chinensis, expelling parasite spot
8 parts of turtledove chrysanthemum, 6 parts of elm bark, 6 parts of calamus.
The preparation method of the biological pesticide of the present embodiment preventing and treating rice pest, comprises the following steps：
1）Bamboo vinegar, chitin and the Chinese medicine composition of above-mentioned weight portion are weighed respectively, then in 30 DEG C of temperature, rotating speed
Carry out mixing 15 minutes under conditions of 2000rpm, stand 15 minutes, obtain mixture；
2）The AVM for weighing above-mentioned weight portion is added in the mixture, and rotating speed 3200rpm is stirred 30 minutes, is stood, i.e.,
?.
Chinese medicine composition described in the present embodiment is obtained by following preparation methods：
1）The Aglaia odorata flower of dry above-mentioned weight, the coptis, Bai Lincao, cuckoo meat, vernonia anthelmintica, elm are weighed respectively
Bark and calamus, mixing, are crushed after freezing under nitrogen protection, obtain comminuting matter I；Weigh respectively dry above-mentioned heavy
The needle juniper and Inula britannica chinensis of amount part proportioning, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 times of its weight is added in the comminuting matter I, 40 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 times of its weight, 50 DEG C are heated to, are kept
38 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.15 medicinal extract, adds the ethanol solution that volume fraction is 80%, temperature in the medicinal extract
25 DEG C, kept for 24 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.25 lotion；
3）The water of its 3 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 35 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
The medicinal extract is 1g with the w/v of the ethanol：12ml.
The using method of the biological pesticide of the preventing and treating rice pest is repeated no more with embodiment 1.
Embodiment 3：A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM hereinafter
15 parts of 0.3 part of rhzomorph, 2 parts of bamboo vinegar, 3 parts of chitin and Chinese medicine composition, original of the Chinese medicine composition by following weight parts
Material is made：Aglaia odorata is spent 20 parts, 20 parts of the coptis, 8 parts of needle juniper, white drenches 12 parts of grass, 8 parts of cuckoo meat, 8 parts of Inula britannica chinensis, expelling parasite ringdove
6 parts of chrysanthemum, 7 parts of elm bark, 7 parts of calamus.
The preparation method of the biological pesticide of the present embodiment preventing and treating rice pest, comprises the following steps：
1）Bamboo vinegar, chitin and the Chinese medicine composition of above-mentioned weight portion are weighed respectively, then in 30 ~ 40 DEG C of temperature, rotating speed
Carry out mixing 10 minutes under conditions of 2200rpm, stand 20 minutes, obtain mixture；
2）The AVM for weighing above-mentioned weight portion is added in the mixture, and rotating speed 3000rpm is stirred 40 minutes, is stood, i.e.,
?.
Chinese medicine composition described in the present embodiment is obtained by following preparation methods：
1）The Aglaia odorata flower of dry above-mentioned weight, the coptis, needle juniper, cuckoo meat, vernonia anthelmintica, elm are weighed respectively
Skin and calamus, mixing, are crushed after freezing under nitrogen protection, obtain comminuting matter I；Dry above-mentioned weight is weighed respectively
The white pouring grass of part proportioning and Inula britannica chinensis, mixing, crush, obtain comminuting matter II；
2）The water of 6 times of its weight is added in the comminuting matter I, 50 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 3 times of its weight, 60 DEG C are heated to, are kept
40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.12 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
30 DEG C, kept for 20 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.21 lotion；
3）The water of its 5 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 40 minutes, and distillation is extracted 3 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
The medicinal extract is 1g with the w/v of the ethanol：18ml.
The using method of the biological pesticide of the preventing and treating rice pest is repeated no more with embodiment 1.
Embodiment 4：A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM hereinafter
20 parts of 0.1 part of rhzomorph, 0.5 part of bamboo vinegar, 6 parts of chitin and Chinese medicine composition, the Chinese medicine composition is by following weight parts
Raw material is made：Aglaia odorata is spent 10 parts, 30 parts of the coptis, 5 parts of needle juniper, white drenches 5 parts of grass, 5 parts of cuckoo meat, 15 parts of Inula britannica chinensis, expelling parasite spot
3 parts of turtledove chrysanthemum, 12 parts of elm bark, 10 parts of calamus.
The preparation method and using method of biological pesticide in the present embodiment, the preparation method of Chinese medicine composition refer to embodiment
1, repeat no more.
Embodiment 5：A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM hereinafter
5 parts of 0.5 part of rhzomorph, 3 parts of bamboo vinegar, 2 parts of chitin and Chinese medicine composition, raw material of the Chinese medicine composition by following weight parts
Make：Aglaia odorata is spent 30 parts, 10 parts of the coptis, 20 parts of needle juniper, white drenches 20 parts of grass, 20 parts of cuckoo meat, 5 parts of Inula britannica chinensis, expelling parasite ringdove
10 parts of chrysanthemum, 3 parts of elm bark, 12 parts of calamus.
The preparation method and using method of biological pesticide in the present embodiment, the preparation method of Chinese medicine composition refer to embodiment
1, repeat no more.
Embodiment 6：A kind of biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM hereinafter
15 parts of 0.4 part of rhzomorph, 0.8 part of bamboo vinegar, 5 parts of chitin and Chinese medicine composition, the Chinese medicine composition is by following weight parts
Raw material is made：Aglaia odorata is spent 15 parts, 25 parts of the coptis, 15 parts of needle juniper, white drenches 15 parts of grass, 15 parts of cuckoo meat, 6 parts of Inula britannica chinensis, expelling parasite
12 parts of ringdove chrysanthemum, 10 parts of elm bark, 3 parts of calamus.
The preparation method and using method of biological pesticide in the present embodiment, the preparation method of Chinese medicine composition refer to embodiment
1, repeat no more.
Embodiment 7：The biological pesticide of the preventing and treating rice pest described by the present embodiment, as different from Example 1：Institute
The biological pesticide of preventing and treating rice pest is stated, is also included：1 weight portion of microbial bacterial agent, the microbial bacterial agent is by quality percentage
Constitute than 70% bacillus subtilis and 30% streptomyces microflavus of mass fraction.
Bacillus subtilis, Bacillus subtilis, living bacteria count >=100,000,000,000 cfu/g；Streptomyces microflavus,
Streptomyces microflavus, living bacteria count >=1.5 hundred million/g；Above-mentioned microbial inoculum can be bought by market channel
Arrive.
The biological pesticide of the present embodiment preventing and treating rice pest is as different from Example 1：Step 2）AVM hereinafter is weighed respectively
Rhzomorph and microbial bacterial agent are added in the mixture, and rotating speed 3000rpm is stirred 40 minutes, are stood, are obtained final product.
Embodiment 8：Described by the present embodiment preventing and treating rice pest biological pesticide, with embodiment 1 except that：
The biological pesticide of the preventing and treating rice pest, also includes：2 weight portion of microbial bacterial agent, the microbial bacterial agent is by quality point
Several 65% bacillus subtilises and 35% streptomyces microflavus of mass fraction composition.
The biological pesticide of the present embodiment preventing and treating rice pest is as different from Example 1：Step 2）AVM hereinafter is weighed respectively
Rhzomorph and microbial bacterial agent are added in the mixture, and rotating speed 3000rpm is stirred 35 minutes, are stood, are obtained final product.
Comparative example 1：The biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：AVM
0.2 part, 1.5 parts of bamboo vinegar, 3.5 parts of chitin.
Comparative example 2：The biological pesticide of preventing and treating rice pest, is prepared from by the raw material of following weight portion：Aglaia odorata is spent
25 parts, 18 parts of the coptis, 10 parts of needle juniper, white drench 10 parts of grass, 10 parts of cuckoo meat, 10 parts of Inula britannica chinensis, 7 parts of vernonia anthelmintica, elm bark 8
Part, 8 parts of calamus.
Effect assessment
1st, field insecticidal effect
1.1 experimental design
9 process of field reagent experimental design, respectively：
Experimental group：A1~A6：It is followed successively by the biological pesticide of the preparation of embodiment 1 ~ 2,3 ~ 4,7 ~ 8；
Control group：B1~B2：It is followed successively by the biological pesticide of the preparation of comparative example 1 ~ 2；B3：Non- dispenser is used as blank.
Often process and be repeated 3 times, altogether 27 cells, be 49m per plot area2, random alignment.
1.2 efficacy survey
Investigation of the investigation using fixed point singling, before dispenser each cell using the sampling of 5 points of diagonal, per choose have white backed planthopper,
15 plants of the paddy rice of the snout moth's larva of rice and rice leaf roller, the quantity of per plant of various insects of statistics that are listed, used as insect population before cell dispenser
Radix.1 after dispenser, 3,7d investigate the amount of survival of each insect on fixed point host crop, statistics hundred plants of number of pest and worm respectively
Mouth decline rate, calculates each process prevention effect, and to correct Revision insect recluced rate as prevention effect, formula is as follows：
Revision insect recluced rate (%)=（Borer population living after borer population-dispenser living before dispenser）Borer population × 100% living before/dispenser,
Prevention effect (%)=（Treatment region Revision insect recluced rate-check plot Revision insect recluced rate）/（100- check plots Revision insect recluced rate）×
100%.
1.3 result of the test
The field control effect of 1 eight kinds of agricultural chemicals Whitebacked Planthoppers of table
As can be seen from Table 1, for the present invention, in the 1d for treating, the prevention effect of embodiment A5 ~ A6 is best, secondly
Prevention effect for embodiment A1 ~ A2, A3 and A4 is above 85%, Whitebacked Planthopper to knock down speed fast；In the 3d for treating,
, more than 95%, fluctuation is less for the prevention effect of embodiment A1 ~ A6；In the 7d for treating, the preventing and treating of embodiment A1 ~ A2 and A5 ~ A6
Effect reaches 100%.For comparative example, B1 ~ B2 is respectively less than 35% in the prevention effect of 1d, and difference is obvious；Applying
The 3d for controlling, although prevention effect increases, still low, not less than 40%；In the 7d for treating, the preventing and treating of B1, B2
Effect is followed successively by 58.7% and 51.7%, not less than 60%, hence it is evident that relatively low.
Above-mentioned contrast can see that the prevention effect of the embodiment of the present invention is significantly better than comparative example, and prevention effect
Quickly, stable.The prevention effect performance of insecticide Whitebacked Planthopper of the present invention is excellent, and highest can reach 100%, hence it is evident that be better than
Mixture component, and than mixture component preventive effect faster, more stable.Insecticide Synergistic of the present invention, effect is significant.
Field control effect of the 2 eight kinds of agricultural chemicals of table to the snout moth's larva of rice
As can be seen from Table 2, biological pesticide of the present invention has stronger killing ability for the preventing and treating snout moth's larva of rice, in for treating
5 days, prevention effect was well beyond 90%, and it is very little to fluctuate；At the 7th day treated, the prevention effect of section Example was
100% is reached, effect is projected very much.And by contrast, then gap is quite big for the prevention effect of comparative example 1 ~ 2, to the preventing and treating of the 7th day
Effect is only proximate to 60%.In general, the prevention effect of the embodiment of the present invention is significantly better than comparative example, and prevention effect is fast
Speed is stable.Insecticide of the present invention is excellent to the prevention effect performance of the snout moth's larva of rice, and highest can reach 100%, hence it is evident that better than mixed
Match somebody with somebody component, and than mixture component preventive effect faster, more stable.Insecticide Synergistic of the present invention, effect is significant.
Field control effect of the 3 eight kinds of agricultural chemicals of table to rice leaf roller
As can be seen from Table 3, biological pesticide of the present invention for preventing and treating rice leaf roller also have extraordinary effect, although comparing
The prevention effect of the snout moth's larva of rice and white backed planthopper somewhat shows weak, but still with the obvious advantage, average prevention effect more than 95%, part
The prevention effect of embodiment can reach 100%.By contrast, the biological pesticide prevention effect that prepared by comparative example 1,2 is then relatively more slow
Slowly, comparative example 1 is in the prevention effect only 30% or so of the 1st day, comparative example 2 then only 21.2%, prolongation over time, contrast
Example 1,2 is only respectively 57.5% and 51.7% to prevention effect when the 7th day.All in all, the prevention effect of the embodiment of the present invention
Comparative example is significantly better than, and prevention effect is quick, stable.Insecticide of the present invention is showed to the prevention effect of rice leaf roller
Excellent, highest can reach 100%, hence it is evident that better than mixture component, and than mixture component preventive effect faster, more stable.The present invention
Insecticide Synergistic, effect is significant.
2nd, disease resisting effect
2.1 reagent agent
Experimental group：A1~A6：It is followed successively by the biological pesticide of the preparation of embodiment 1 ~ 2,3 ~ 4,7 ~ 8；
Control group：B1~B2：It is followed successively by the biological pesticide of the preparation of comparative example 1 ~ 2；B3：Clear water.
Often process and be repeated 3 times.
2.2 test method
Adopt above-mentioned reagent agent to make the aqua that mass percent is for 2%, carry out immersion treatment respectively to rice paddy seed, and divide
Seedling stage not in paddy rice, bloom before and Post flowering carry out spraying processs, the interval time for spraying process is 30 days.Each process is logical
Cross 3 pieces of experimental plots to be observed and detected, every piece of experimental plot area is identical, is 100 square metres.The sample for processing seed is being made
Grow to florescence after thing germination to be counted, for the statistics of each phase process in growth period is equally waited until into after administration
The ripe phase is counted.Percentage that the paddy rice diseased plant in every piece experimental plot occur is counted respectively, as long as having characteristics of lesion just should
Plant is defined as pathology plant.
2.3 result of the test
Table 4 is whole to process prevention effect of the paddy rice to rice sheath blight disease
Table 5 is whole to process prevention effect of the paddy rice to rice blast
Table 6 is whole to process prevention effect of the paddy rice to stripe virus disease
By table 4 ~ 6 as can be seen that biological pesticide of the present invention can show more excellent resisting in the whole process use of Rice Cropping
Sick effect, and each Stage control effect constantly adds up, can Eradication stripe disease evil, make sending out for rice sheath blight disease
Raw rate is significantly reduced.And, the pathology plant percentage after each phase process is contrasted it is obvious that the biology of the present invention
Agricultural chemicals has obvious prevention effect to paddy rice to stripe virus disease.
The above results show that biological pesticide of the present invention is done harm to rice sheath blight disease, rice blast and stripe virus disease, no matter
It is Seed Treatment, before seedling stage processes, blooms process or bloom post processing, shows excellent disease-resistant performance, especially
The better protrusion of stripe virus disease evil is prevented and treated, biological pesticide of the present invention can be used in each stage of Rice Cropping.
Although the foregoing describing the specific embodiment of the present invention, those familiar with the art should manage
Solution, the specific embodiment described by us are merely exemplary, rather than for the restriction to the scope of the present invention, are familiar with this
Equivalent modification and change that the technical staff in field is made in the spirit according to the present invention, should all cover the present invention's
In scope of the claimed protection.
Claims (8)
1. a kind of preventing and treating rice pest biological pesticide, it is characterised in that：It is prepared from by the raw material of following weight portion：AVM hereinafter
5 ~ 20 parts of 0.1 ~ 0.5 part of rhzomorph, 0.5 ~ 3 part of bamboo vinegar, 1 ~ 5 part of chitin and Chinese medicine composition, the Chinese medicine composition is by under
The raw material for stating weight portion is made：Aglaia odorata is spent 10 ~ 30 parts, 10 ~ 30 parts of the coptis, 5 ~ 20 parts of needle juniper, white drenches careless 5 ~ 20 parts, cuckoo meat
5 ~ 20 parts, 5 ~ 15 parts of Inula britannica chinensis, 3 ~ 12 parts of vernonia anthelmintica, 3 ~ 12 parts of elm bark, 3 ~ 12 parts of calamus.
2. as claimed in claim 1 preventing and treating rice pest biological pesticide, it is characterised in that：The Chinese medicine composition is by under
The raw material for stating weight portion is made：Aglaia odorata is spent 20 ~ 25 parts, 15 ~ 20 parts of the coptis, 8 ~ 12 parts of needle juniper, white drenches careless 8 ~ 12 parts, cuckoo meat
8 ~ 12 parts, 8 ~ 12 parts of Inula britannica chinensis, 6 ~ 8 parts of vernonia anthelmintica, 6 ~ 8 parts of elm bark, 6 ~ 8 parts of calamus.
3. as claimed in claim 2 preventing and treating rice pest biological pesticide, it is characterised in that：The Chinese medicine composition is by under
The raw material for stating weight portion is made：Aglaia odorata spend 25 parts, 18 parts of the coptis, 10 parts of needle juniper, white 10 parts of the grass, 10 parts of cuckoo meat, sheathed of drenching
Spend 10 parts, 7 parts of vernonia anthelmintica, 8 parts of elm bark, 8 parts of calamus.
4. as claimed in claim 3 preventing and treating rice pest biological pesticide, it is characterised in that：Raw material by following weight portion
It is prepared from：10 ~ 15 parts of 0.2 ~ 0.3 part of AVM, 1 ~ 2 part of bamboo vinegar, 3 ~ 4 parts of chitin and Chinese medicine composition.
5. as described in any one of claim 1 ~ 4 preventing and treating rice pest biological pesticide, it is characterised in that：The Chinese medicine group
Compound is obtained by following preparation methods, and the preparation method is comprised the following steps：
1）The Aglaia odorata flower of dry above-mentioned weight, the coptis, Bai Lincao, cuckoo meat, vernonia anthelmintica, elm are weighed respectively
Bark and calamus, mixing, are crushed after freezing under nitrogen protection, obtain comminuting matter I；Weigh respectively dry above-mentioned heavy
The needle juniper and Inula britannica chinensis of amount part proportioning, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 ~ 3 times of its weight, it is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 medicinal extract, adds volume fraction in the medicinal extract
For 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, kept for 18 ~ 24 hours, then filter, obtain filtrate III and sediment, by institute
State the lotion that relative density when filtrate III is evaporated to 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with dichloromethane, sub-cloud solution, vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain final product.
6. as claimed in claim 5 preventing and treating rice pest biological pesticide, it is characterised in that：The medicinal extract and the ethanol
W/v 1g：10~20ml.
7. as claimed in claim 5 preventing and treating rice pest biological pesticide, it is characterised in that：The organic solvent is dichloro
Methane or chloroform.
8. a kind of preventing and treating rice pest biological pesticide preparation method, it is characterised in that：Comprise the following steps：
1）Bamboo vinegar 0.5 ~ 3 part, chitin 2 ~ 6 part and the Chinese medicine composition 5 ~ 20 part of weight portion are weighed respectively, then in temperature
30 ~ 40 DEG C, carry out mixing 10 ~ 15 minutes under conditions of 2000 ~ 2500rpm of rotating speed, stand 15 ~ 20 minutes, mixed
Thing；
2）0.1 ~ 0.5 part of AVM for weighing weight portion is added in the mixture, 3000 ~ 3200rpm of rotating speed stirrings 30 ~ 40
Minute, stand, obtain final product.
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