CN102204576A - Method for relieving damage of hirschmanniella oryzae to rice by using Agrimonia pilosa Ledeb. extract - Google Patents
Method for relieving damage of hirschmanniella oryzae to rice by using Agrimonia pilosa Ledeb. extract Download PDFInfo
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- CN102204576A CN102204576A CN2011100847480A CN201110084748A CN102204576A CN 102204576 A CN102204576 A CN 102204576A CN 2011100847480 A CN2011100847480 A CN 2011100847480A CN 201110084748 A CN201110084748 A CN 201110084748A CN 102204576 A CN102204576 A CN 102204576A
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Abstract
The invention belongs to the technical field of hirschmanniella oryzae prevention and control and relates to a method for relieving damage of hirschmanniella oryzae to rice by using Agrimonia pilosa Ledeb. extract, which comprises: grinding and sieving Agrimonia pilosa Ledeb.; extracting by using water or an organic solvent as an entrainer to obtain the extract; and directly spraying diluted Agrimonia pilosa Ledeb. extract or mixture of the Agrimonia pilosa Ledeb. extract, a pesticide and a chemical fertilizer onto rice or dipping the roots of seedlings into the diluted Agrimonia pilosa Ledeb. extract or the mixture of the Agrimonia pilosa Ledeb. extract, a pesticide and a chemical fertilizer during transplanting. The Agrimonia pilosa Ledeb. extract can relive the damage of the hirschmanniella oryzae to rice, so yield and harvest can be increased. The hirschmanniella oryzae is benefit for safety of organisms and environment-friendly.
Description
Invention field
The invention belongs to paddy rice nematodiasis biological control technical field, be specifically related to a kind of hairyvein agrimony (Agrimonia pilosa Ledeb.) extract that utilizes and alleviate the method for latent root nematode rice hazard, more specifically, the present invention relates to the use of Herba Agrimoniae extract on paddy rice.
Background technology
The paddy rice root nematode of diving has become global paddy rice pest, and it infects the disease that paddy rice causes and causes the serious underproduction and be difficult to control.Adopt the method for rice field-upland field rotation can effectively alleviate disease and take place, but helpless to rotate crop varieties single, and because rice is the daily staple food grains of people, long-term rice field-upland field rotation reduces paddy rice cultivated area thing and relates to grain security and social stability and be difficult to continue to carry out; Adopt chemical agent can retrieve partial loss in conjunction with the agronomic measures control; But along with social progress and the people concern to healthy and environment, the limitation and the drawback of chemical agent use day by day manifest; More and more pay attention to today of environmental problem and pollution-free food production people, environmental pollution is serious, disabled in many countries and regions successively to high poison, height chemical nematode killing agent residual and " three cause " that human and livestock health is harmful, as once being widely used in medicament-furans pellet, Nemacur, the ethoprophos of rice field control nematode.It is important that the screening of biogenic prevention and control medicament more shows.Yet microorganism such as using fungus, bacterium, food line nematode and product thereof, the plant resource active component control paddy rice preparation that root nematode disease is applied to produce of diving does not still have open report, still relies on chemical agent on control in field.
Since century, the agricultural science and technology worker has done a large amount of tests and research to the control of the latent root nematode of paddy rice, can reduce following several respects more than one: 1. agricultural measures rice field-upland field rotation is effective measures to the control of the root nematode of diving.But root nematode host range is very extensive owing to dive, and the object of crop rotation is very limited.Disease tolerant variety: in to paddy rice and the interrelationship study of root nematode, do not see that as yet a certain rice varieties is to the high anti-report of the root nematode of diving.2. the chemical agent chemical agent of preventing and treating the rice root nematode prevent and treat optimum period be period of seedling establishment to beginning the tiller phase, secondly be the transplanting phase; Use ethoprophos 5%SG75kghm
-2The dispenser in tillering stage can make increasing production of rice 4.2%-10.4%.Use the nematode population 48.7%, 46.2% and 40.8%, 40.0% of carbosulfan, phosphamidon processing can the minimizing respectively soil and latent root.3. the utilization that presses down line plant and fungus presses down the line plant: foreign study finds that the plant that can prevent and treat nematodiasis has reached 47 kinds of 41 genus, screened multiple to the effective fungal metabolite of nematode, the domestic report that also has pick up the ears (Pleurotus ostreatus), shaggy cap and macro fungi to kill the line activity.But all be limited to the result of study under the stripped state of nematode in the laboratory.
Botanical pesticide is different from the agricultural chemicals of chemosynthesis, it derives from plant, Environmental compatibility is good, can not cause new pressure to environment, the main performance that agricultural chemicals had of its existing chemosynthesis, the advantage that simultaneously again chemical synthetic pesticide is arranged and do not had, it can not cause new pollution to environment in the whole ecological system.China's plant resources is abundant, and China's development botanical pesticide has resources advantage.
Summary of the invention
The object of the present invention is to provide the root nematode prevention and control agent of diving of plant resource paddy rice,, can strengthen the tolerance of paddy rice, alleviate the root nematode of diving, reach the purpose of increasing both production and income the harm of paddy rice to the root nematode of diving by using this prevention and control agent.
Of the present invention being characterized as: a kind of material as nematode prevention and control agent is the extract of Chinese medicine hairyvein agrimony (Agrimonia pilosa Ledeb.), said Herba Agrimoniae extract is that hairyvein agrimony obtains by lixiviate or extraction, lixiviate or extraction adopt water or organic solvent to do entrainer, wherein water can be natural water, distilled water etc., and organic solvent can be in methyl alcohol, ethanol, propyl alcohol, butanols, amylalcohol, benzinum, chloroform, ethyl acetate, the acetone one or more.Extracting method is undertaken by following method: the dry hairyvein agrimony herb of (1) material selection, and low-temperature grinding is crossed 20～50 mesh sieves; (2) hairyvein agrimony after will pulverizing carries out lixiviate or extraction, is entrainer with the organic solvent, and as ethanol, its usage amount is 5～10 times of powder total amount, by the 45 ℃ of water-bath rotary evaporations that reduce pressure concentrate extract; With water is entrainer, and as distilled water, its usage amount is 10～15 times of powder total amount, by the 60 ℃ of water-bath rotary evaporations that reduce pressure concentrate extract;
Alleviating the method for latent root nematode to rice hazard, is with the prevention and control agent of Herba Agrimoniae extract as the root nematode of diving, and improves the tolerance of paddy rice to latent root eelworm harm.
Be 0.001g～50g/m at amount of application when Herba Agrimoniae extract sprays
2The time to rice safety, when being stained with the seedling root amount of application when being 0.001g～100g/kg to rice safety.
Described Herba Agrimoniae extract comprises the composition pesticide that itself and agricultural chemicals or Fertilizer Combination are made, and Herba Agrimoniae extract and other auxiliary agents are made preparation.
The extract of hairyvein agrimony directly is sprayed on rice leaf or the cane after diluting; Or directly when shoot transplanting equipment, be stained with the seedling root.
Contain the preparation of Herba Agrimoniae extract and auxiliary agent, or contain the composite back of described Herba Agrimoniae extract and other agriculture chemicals composition sprayed on paddy rice; Or when shoot transplanting equipment, be stained with the seedling root.
Herba Agrimoniae extract can alleviate the root nematode of diving to the injury of paddy rice, is example with the ethanol extract, and in pot experiment, when the root nematode of diving connect the worm amount and is 100/strain, amount of application was 1,2,4,8,16,32,64g/m
2Herba Agrimoniae extract is respectively 1.81%, 1.98%, 2.59%, 3.86%, 6.47%, 6.79%, 6.59% to the facilitation of rice yield.
Herba Agrimoniae extract can alleviate the root nematode of diving to the injury of paddy rice, is example with the distilled water extract, and in pot experiment, when the root nematode of diving connect the worm amount and is 100/strain, amount of application was 4,16,64,288g/m
2Herba Agrimoniae extract is respectively 2.12%, 3.44%, 7.60%, 5.92% to the facilitation of rice yield.
Herba Agrimoniae extract can reduce the root nematode of diving to the infection rate of paddy rice, is example with the ethanol extract, and in pot experiment, when the root nematode of diving connect the worm amount and is 200/strain, amount of application was 2,4,8,16,32,64,128g/m
2Herba Agrimoniae extract, during results with do not execute extract relatively, the insect population rate of going down is respectively 22.5%, 28.9%, 34.5%, 48.6%, 55.6%, 61.5%, 66.3%.
Advantage of the present invention and characteristics: obvious effect of increasing production, biological activity determination shows in a large amount of greenhouses, and the root nematode of diving produces harm to paddy rice, after the process Herba Agrimoniae extract is handled paddy rice, can obviously alleviate of the harm of latent root nematode, have tangible effect of increasing production paddy rice.Behind Herba Agrimoniae extract processing paddy rice, can improve the anti-infectivity of paddy rice to the root nematode of diving, invade the interior quantity of rice root thereby reduce the root nematode of diving.Hairyvein agrimony belongs to vegetable material, and good with Environmental compatibility, its extract is applied to paddy rice, to rice safety, also can not pollute environment.
Embodiment
Embodiment 1
Herba Agrimoniae extract improves paddy rice to the anti-infectivity of root nematode of diving.The following examples are to further specify of the present invention, below are the results of indoor bioassay, but should regard the restriction of by any way range of application of the present invention being carried out as in any case.
Select dry hairyvein agrimony herb for use, low-temperature grinding is crossed 20～50 mesh sieves; Hairyvein agrimony after pulverizing is carried out lixiviate or extraction, is entrainer with the organic solvent, and as ethanol, its usage amount is 10 times of powder total amount, by the 45 ℃ of water-bath rotary evaporations that reduce pressure concentrate extract; (about 10g hairyvein agrimony can get the 1g extract), 4 ℃ of refrigerators are preserved.
Load onto the 200g air dried soil at the plastic casing of 13cm * 8cm * 5cm, add 100ml nature water, the seed soaking 24 hours down of 25 ℃ of rice paddy seeds, 30 ℃ of following vernalization 12 hours is chosen seed of the same size to be sowed in the plastic casing after showing money or valuables one carries unintentionally, 15 in every box, place 25 ℃ of temperature daytimes, at 22 ℃ of nights, intensity of illumination is to cultivate in the illumination box of 6000Lux, day-night cycle is 12 hours, treats to inoculate when paddy rice two leaves wholeheartedly processing.
With dive 1500 of root nematodes of every box inoculation, again with Herba Agrimoniae extract according to 4,8,16,32,64g/m
2Five consumptions are handled respectively, and each is handled 4 times and repeats, and complete blank and inoculation contrast are set, and continue to get root and leak worm according to above-mentioned condition illumination cultivation 15 days.Herba Agrimoniae extract improves paddy rice to the anti-infectivity of root nematode of diving.The result is as shown in table 1.
Table 1 Herba Agrimoniae extract improves paddy rice to the anti-infectivity of root nematode of diving
Data are four repetition mean values in the last table
Embodiment 2
Herba Agrimoniae extract alleviates the land for growing field crops evaluating drug effect of root nematode to rice hazard of diving.
Extracting method: select dry hairyvein agrimony herb for use, low-temperature grinding is crossed 20～50 mesh sieves; Hairyvein agrimony after pulverizing is carried out lixiviate or extraction, is entrainer with water, and as distilled water, its usage amount is 15 times of powder total amount, filters by 3 lixiviates of 60 ℃ of water-baths of reducing pressure, and rotary evaporation gets extract after the collection concentrating filter liquor; (about 10g hairyvein agrimony can get the 1g extract), 4 ℃ of refrigerators are preserved.
Rice varieties: Lou You 207
The experimental field root nematode radix of diving: 7450/100 gram roots
Paddy rice is on June 22nd, 2010 sowing seedling, treats that rice shoot length transplants to four leaf phases, plants and carries out chemicals treatment in back 10 days, and processing method is that rice shoot is carried out even spraying, and amount of application is 1,4,8,16,32,64,128g/m
2Seven dosage, contrast medicament A Wei 1.8%EC 0.15ml/m
2, 4 repetitions are provided with not dispenser blank; Worm is leaked in sampling in 40 days after the chemicals treatment, surveys during results and produces.
Table 2 Herba Agrimoniae extract alleviates the land for growing field crops evaluating drug effect of root nematode to rice hazard of diving
Data are four repetition mean values in the last table, output and worm amount round number.
Embodiment 3
Herba Agrimoniae extract alleviates the land for growing field crops evaluating drug effect of root nematode to rice hazard of diving.
Extracting method: select dry hairyvein agrimony herb for use, low-temperature grinding is crossed 20～50 mesh sieves; Hairyvein agrimony after pulverizing is carried out lixiviate or extraction, is entrainer with the organic solvent, and as acetone, its usage amount is 10 times of powder total amount, filters by 3 lixiviates of 40 ℃ of water-baths of reducing pressure, and rotary evaporation gets extract after the collection concentrating filter liquor; (about 8g hairyvein agrimony can get the 1g extract), 4 ℃ of refrigerators are preserved.
Rice varieties: Shan excellent 256
The experimental field root nematode radix of diving: 5560/100 gram roots
Paddy rice is on April 22nd, 2009 sowing seedling, treats that rice shoot length transplants to four leaf phases, carries out chemicals treatment during transplanting, processing method is the rice shoot root to be stained with root handle, be stained with the seedling root with the medicine fertilizer that contains Herba Agrimoniae extract 20-50g/kg, the contrast medicament contains A Wei 0.1g/kg, and not dispenser blank is set; Transplant the back and observed the seedling growth situation in 3,7,14,20 days, worm is leaked in sampling after 45 days.
Seedling growth situation observed result shows that extract is stained with root seedling growth is had no adverse effects.
Leak the worm result and show, be stained with the root processing and can obviously reduce nematode intrusion amount, it is suitable with the contrast pharmacy effect that 20g/kg handles.
Claims (3)
1. one kind is utilized Herba Agrimoniae extract to alleviate the method for latent root nematode to rice hazard, it is characterized in that: directly be sprayed on the rice leaf and cane in various vegetative period after the said Herba Agrimoniae extract dilution, or be sprayed on rice leaf and the cane after Herba Agrimoniae extract and fertilizer or the pesticide combination, Herba Agrimoniae extract is 0.001～50g/m at amount of application
2The time to rice safety.
2. one kind is utilized Herba Agrimoniae extract to alleviate the method for latent root nematode to rice hazard, it is characterized in that: the rice shoot that directly applies to when transplanting after the said Herba Agrimoniae extract dilution is stained with root, or when shoot transplanting equipment, be stained with root after Herba Agrimoniae extract and fertilizer or the pesticide combination, Herba Agrimoniae extract when amount of application is 0.001～100g/kg to rice shoot safety.
3. a kind of Herba Agrimoniae extract that utilizes according to claim 1 alleviates the method for latent root nematode to rice hazard, and it is characterized in that: described latent root nematode comprises one or more in the root Turbatrix (Hirschmanniella spp) of diving.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726461A (en) * | 2012-06-18 | 2012-10-17 | 湖南人文科技学院 | Method of mitigating harm of Hirschmanniella to paddy rice by using extract product of Perilla frutescens (L.) Britt |
| CN102896034A (en) * | 2012-08-22 | 2013-01-30 | 王丽华 | High-flux plant leaf crusher |
| CN104542638A (en) * | 2014-10-21 | 2015-04-29 | 泰安市泰山林业科学研究院 | Plant source preparation for preventing and controlling agrobacterium tumefaciens conn and preparation method of plant source preparation |
| CN106083398A (en) * | 2016-07-27 | 2016-11-09 | 吴春凤 | A kind of nutritional solution preventing Fructus actinidiae chinensis blossom-end rot |
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| WO2001089298A2 (en) * | 2000-05-19 | 2001-11-29 | Vanderbilt University | Compositions and methods of nematode control |
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Patent Citations (2)
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| WO2001089298A2 (en) * | 2000-05-19 | 2001-11-29 | Vanderbilt University | Compositions and methods of nematode control |
| CN101518249A (en) * | 2009-04-10 | 2009-09-02 | 湖南美可达生物资源有限公司 | Phyto-pesticide and use method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726461A (en) * | 2012-06-18 | 2012-10-17 | 湖南人文科技学院 | Method of mitigating harm of Hirschmanniella to paddy rice by using extract product of Perilla frutescens (L.) Britt |
| CN102896034A (en) * | 2012-08-22 | 2013-01-30 | 王丽华 | High-flux plant leaf crusher |
| CN104542638A (en) * | 2014-10-21 | 2015-04-29 | 泰安市泰山林业科学研究院 | Plant source preparation for preventing and controlling agrobacterium tumefaciens conn and preparation method of plant source preparation |
| CN106083398A (en) * | 2016-07-27 | 2016-11-09 | 吴春凤 | A kind of nutritional solution preventing Fructus actinidiae chinensis blossom-end rot |
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Application publication date: 20111005 |