CN106538611A - From bactericide its preparation method and the application of frutus cnidii - Google Patents
From bactericide its preparation method and the application of frutus cnidii Download PDFInfo
- Publication number
- CN106538611A CN106538611A CN201610914700.0A CN201610914700A CN106538611A CN 106538611 A CN106538611 A CN 106538611A CN 201610914700 A CN201610914700 A CN 201610914700A CN 106538611 A CN106538611 A CN 106538611A
- Authority
- CN
- China
- Prior art keywords
- frutus cnidii
- bactericide
- verticillium wilt
- mixed
- frutus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to field of biological pesticide, and in particular to from bactericide its preparation method and the application of frutus cnidii.The bactericide includes the organic silicon surfactant of the common cnidium fruit P.E and 1wt%～3wt% of 97wt%～99wt%.The bactericide can effectively suppress cotton verticillium wilt cause of disease mycelial growth and Spores to sprout, and then effectively prevent and treat cotton verticillium wilt, provide a kind of botanical pesticide of efficient, low toxicity, low-residual effectively to prevent and treat cotton verticillium wilt.
Description
Technical field
The present invention relates to field of biological pesticide, and in particular to from frutus cnidii bactericide its preparation method and should
With.
Background technology
Cotton verticillium wilt is the native transmissibility tracheomycosis evil caused by verticillium dahliae, have a strong impact on output of cotton and
Fiber quality.About 80,000,000 mu of the annual plant cotton area of China, due to long-term continuous cropping, in soil, verticillium wilt pathogen is increasingly accumulated,
And lack disease-resistant variety and the agricultural chemicals with preferable prevention effect, cause and sky is almost to the preventing and treating of verticillium wilt in production at present
In vain, the occurrence injury of disease is on the rise, and becomes one of main limiting factor of DEVELOPMENT OF COTTON production.
Botanical pesticide be the activity that extracted using the part such as plant roots, stem, leaf, flower, fruit and seed or be separated to into
Divide the preparation being processed into.Botanical pesticide has the characteristics that：(1) with multiple biological activities, not only insecticidal activity, also and
There is activity that is sterilized and stimulating plant growth；(2) it is low to people and animals and non-target organism toxicity, degrade in the environment fast, low-residual,
It is free from environmental pollution；(3) insect is not likely to produce resistance；(4) raw material is easy to get, and can gather materials on the spot, process on the spot, use cost compared with
It is low.The utilization of botanical pesticide can be divided in terms of two, and one is directly to utilize, i.e., the active material in plant is slightly extracted
Afterwards, directly it is processed into available preparation.Major advantage is that for this Land use systems plays various composition in crude extract
Synergy, and small investment, construction cycle are short；Two is indirect utilization, i.e. the structure of research active material, mechanism of action, knot
Relation between structure and activity, and then manual simulation's synthesis screening, so as to develop new pesticidal preparations.At present, plant source agriculture
Medicine is increasingly favored by people, is the emphasis direction of ecological agricultural chemical development from now on.
At present, research of the botanical pesticide in terms of cotton verticillium wilt preventing and treating is less, only S-Ethyl ethylthio sulfonate.S-Ethyl ethylthio sulfonate has big
Garlic and acetic acid stink, high volatility, have severe corrosive；And it is relatively low to the prevention effect of cotton verticillium wilt, such as 2006 and 2007
Year, being undertaken in national agriculture chemical registration test by the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute, Henan Province's the earth agrochemical Limited Liability is public
80% S-Ethyl ethylthio sulfonate missible oil of department's production is 30.8%-32.0% to the prevention effect of cotton verticillium wilt；Therefore the product is in cotton yellow
Application in terms of disease of withering preventing and treating is little.At present, the biologic product in domestic production using more preventing and treating cotton verticillium wilt is main
There are amino-oligosaccharide and bacillus subtilis.These biological agents are relatively low to the prevention effect of cotton verticillium wilt.For example, by China
Academy of Agricultural Sciences's Cotton Research Institute is undertaken in national agriculture chemical registration test, the life of Shaanxi Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd
The 0.5% amino acid oligosaccharide for producing is 31.5%-37.6% (2012 and 2013) to the prevention effect of cotton verticillium wilt, but
In large area test in 2014, prevention effect only has 26.7%；Heilungkiang your biochemical technology development zone Co., Ltd strong produces
100000000000 living spores/gram bacillus subtilis wettable powder is 46.0%-52.9% to the prevention effect of cotton verticillium wilt
(2008 and 2009), but in large area test in 2010, prevention effect only has 32.7%.
Environmentally friendly, the efficient botanical pesticide of exploitation is the important process of the sustainable control of agricultural disease, meets green, environmental protection
Requirement.Frutus cnidii [Cnidium monnieri (L.) Cuss.], another name cicely, is the drying of samphire cnidium monnieri
Ripening fruits.Harvest when summer, two season of autumn fruit maturation, remove impurity, dry.Which is warm in nature, bitter.With clearing damp, detoxify, only
Itch, it is adaptable to neurodermatitis, pruitus, chronic eczema, the effects such as ringworm etc., but which is used as bactericidal agent for preventing and treating cotton yellow
There is not been reported for the effect for disease of withering.The present invention is withered to cotton yellow by the extracting method of effective sterilization component in research frutus cnidii
Germ inhibitory action and the proportioning with auxiliary agent, field is using technology etc., there is provided a kind of to can be used for what cotton verticillium wilt was prevented and treated
The preparation method of botanical pesticide, has tremendous potential to the effective control of cotton verticillium wilt.
The content of the invention
It is an object of the invention to provide a kind of bactericide with common cnidium fruit P.E as active material, answering as bactericide
With can effectively prevent and treat cotton verticillium wilt.
It is an object of the invention to provide from the bactericide of frutus cnidii.
Another object of the present invention is to provide the method for preparing the above-mentioned bactericide from frutus cnidii.
Another object of the present invention is to provide the application of the above-mentioned bactericide from frutus cnidii.
Bactericide from frutus cnidii of the invention, including the common cnidium fruit P.E of 97wt%～99wt%
With the organic silicon surfactant of 1wt%～3wt%, the organic silicon surfactant is the activity that a class contains organosilicon
Agent, the silicone active agent main component for the application are modified poly- trisiloxanes for ethyoxyl, and formulation is missible oil, according to this Shen
Specific embodiment please, the outstanding effect profit of trade name of the silicone active agent, manufacturer is that the U.S. steps figure new high-tech material collection
Group, is sold by Qingdao Hua Ken Import and Export Co., Ltd.s, wherein, the preparation process of the common cnidium fruit P.E is as follows：
(1) frutus cnidii is dried in 50 DEG C to constant weight, then mechanical crushing is to 40 mesh；
(2) frutus cnidii of above-mentioned crushing is mixed by mass volume ratio 1/2 with ethanol, then leaches extract；
(3) water of the quality volumes such as frutus cnidii dry weight is added in the extract obtained to step (2), is mixed, that is, is obtained snake
Machine tool extract.
Specific embodiment of the invention, the invention provides a kind of preparation method for preparing the bactericide,
Including being made up of frutus cnidii (Cnidium monnieri (L.) Cuss.) extract and organic silicon surfactant processing, should
In bactericide, shared by each component, mass percent is：Common cnidium fruit P.E:97wt%～99wt%, organic silicon surfactant:
1wt%～3wt%.
The method comprises the steps：
(1) frutus cnidii is dried in 50 DEG C to constant weight, then mechanical crushing is to 40 mesh；
(2) frutus cnidii of above-mentioned crushing is mixed in proportion with ethanol, mass volume ratio 1/2, is placed in 25 DEG C of constant-temperature tables
After middle vibration 12 hours, extract is leached with gauze；Filter residue is added into the ethanol of 2 times of volumes, repeats said extracted process 2 times,
Three gained extracts are merged, the water of the quality volumes such as frutus cnidii dry weight is added, is mixed, that is, obtain frutus cnidii crude extract；
(3) by frutus cnidii crude extract 60 DEG C of revolvings of Rotary Evaporators, the quality volume such as frutus cnidii dry weight is concentrated into, is added
1%～3% organic silicon surfactant, that is, obtain frutus cnidii bactericide.
Technology according to the present invention scheme, in step (2), the root of Chinese wild ginger with the mass volume ratio 1/2 and the number of times for extracting of ethanol is
The critical technical parameter of present techniques effect is affected, the content of active ingredient will be affected.
In above-mentioned steps (3), the content of organic silicon surfactant is the key technology ginseng for affecting present techniques effect
Number, will affect prevention effect of the application extract to verticillium wilt.
The bactericide can be applicable to the sprouting of the mycelial growth and Spores for suppressing cotton verticillium wilt pathogen.In cotton
There is phase beginning in verticillium wilt, dispenser dosage is 200mL/ mu -400mL/ mus, and insecticide-applying way is spraying, 1 times a week, continuously sprays 3
It is secondary, cotton verticillium wilt can be prevented and treated.
Description of the drawings
Fig. 1 is inhibitory action (A of the frutus cnidii bactericide to cotton verticillium wilt cause of disease mycelial growth：Process；B：It is blank right
According to).
Specific embodiment
The preparation of 1 frutus cnidii bactericide of embodiment
(1) frutus cnidii is dried in 50 DEG C to constant weight, then mechanical crushing is to 40 mesh；
(2) frutus cnidii of above-mentioned crushing is mixed in proportion with ethanol, mass volume ratio 1/2, is placed in 25 DEG C of constant-temperature tables
After middle vibration 12 hours, extract is leached with gauze；Filter residue is added into the ethanol of 2 times of volumes, repeats said extracted process 2 times,
Three gained extracts are merged, the water of the quality volumes such as frutus cnidii dry weight is added, is mixed, that is, obtain frutus cnidii crude extract；
(3) by frutus cnidii crude extract 60 DEG C of revolvings of Rotary Evaporators, the quality volume such as frutus cnidii dry weight is concentrated into, is added
1%～3% organic silicon surfactant, that is, obtain frutus cnidii bactericide.
Suppression of the 2 frutus cnidii bactericide of embodiment to cotton verticillium wilt cause of disease mycelial growth
The bactericide obtained by the present invention, according to the Activities that research of plant disease method is recommended, to cotton
Verticillium wilt pathogen has carried out biological activity determination：The eugonic pathogen bacterial classification of 5mm colony edges is taken with card punch, is placed on
Above the PDA plate of dosing, using in culture medium add the sterilized water of equivalent as control, be repeated 3 times every time, be placed in 25 DEG C of constant temperature trainings
Support, the diameter of each bacterium colony is determined with crossing method, with its mean value to represent the diameter of bacterium colony, suppression is obtained with following equation
Mycelial growth rate processed：
Suppress mycelial growth rate=(control colony diameter-process colony diameter)/(control colony diameter -5) × 100%
The inhibitory action of table 1, frutus cnidii bactericide to cotton verticillium wilt cause of disease mycelial growth
Result of implementation shows (table 1) that frutus cnidii bactericide has preferably suppression to cotton verticillium wilt pathogen mycelial growth
Make and use (Fig. 1), when frutus cnidii concentration of sterilant reaches 0.04g/mL, bacteriostasis rate reaches 100%.
The inhibitory action that 3 frutus cnidii bactericide of embodiment is sprouted to cotton verticillium wilt Spores
The bactericide obtained by embodiment 1, according to the spore germination assay method that research of plant disease method is recommended, to cotton
Flower verticillium wilt Spores carry out germination rate measure：Cotton after being cultivated 7 days on potato sucrose agar medium with sterilized water
The spore of flower verticillium wilt pathogen is eluted and is vibrated and shakes up, and makes which in microscope low power lens (× 40) by doubling dilution
Number is kept to be 100 or so in the visual field.Using slide sessile drop method, 10 μ L of spore suspension are dropped on concave slide, by difference
Concentration frutus cnidii bactericide dilution takes 10 μ L and is added dropwise in spore suspension, is respectively the final concentration of frutus cnidii extract
0.01g/mL, 0.02g/mL, 0.04g/mL, control group take the distilled water of equivalent and make same process.Spore is observed after 25 DEG C of culture 10h
Sub- sprouting situation, per group includes 5 repetitions, and calculates inhibition of germination.
The inhibitory action that table 2, frutus cnidii bactericide are sprouted to cotton verticillium wilt Spores
Result of implementation shows (table 2) that frutus cnidii bactericide has preferably suppression to the spore germination of cotton verticillium wilt pathogen
Make and use, when frutus cnidii concentration of sterilant reaches 0.04g/mL, inhibition of germination reaches 100%.
Embodiment 4：The application of frutus cnidii bactericidal agent for preventing and treating cotton verticillium wilt
Cotton verticillium wilt controlling experiment：The bactericide obtained by the present invention, according to the side that research of plant disease method is recommended
Method carries out cotton verticillium wilt controlling experiment.
This test is carried out in Cotton Inst., Chinese Agricultural Academy's cotton verticillium wilt grave illness field, occurs in cotton verticillium wilt phase beginning
Start, by bactericide dilute with water, 3 dispenser dosage 100mL/ mus, 200mL/ mus, the spraying of 400mL/ mus are set, continuously spray 3
It is secondary, it is spaced 7 days.Often process and be 4 repetitions, often repeatedly 4 row area, 25.6 ㎡ of plot area, middle two rows are enumeration district, spray
The morbidity radix of front investigation verticillium wilt, afterwards per 7 days once, the 7th day after terminating to last time spray.Calculate disease index and
Protection effect.The prevention effect of each process was evaluated with the investigation result of 1 week after last time dispenser.Diseased plant rate, disease index
It is as follows with the computing formula of prevention effect：
Prevention effect of the 3 frutus cnidii bactericide of table to cotton verticillium wilt disease
Result of implementation (table 3) shows that frutus cnidii bactericide can effectively prevent and treat cotton verticillium wilt, when dispenser dosage is 200mL/
During mu～400mL/ mus, relative control effect is 41.9%～53.5%, is significantly higher than the prevention effect of 0.5% amino-oligosaccharide.
Claims (7)
1. from the bactericide of frutus cnidii, it is characterised in that the bactericide includes the cnidium monnieri of 97wt%～99wt%
The organic silicon surfactant of seed extract and 1wt%～3wt%, the silicone active agent are modified poly- three silica for ethyoxyl
Alkane, wherein, the preparation process of the common cnidium fruit P.E is as follows：
(1) frutus cnidii is dried in 50 DEG C to constant weight, then mechanical crushing is to 40 mesh；
(2) frutus cnidii of above-mentioned crushing is mixed by mass volume ratio 1/2 with ethanol, is then acted on 12 hours in 25 DEG C, filtered,
The water of the quality volumes such as frutus cnidii dry weight is added, is mixed, that is, is obtained common cnidium fruit P.E.
2. the bactericide from frutus cnidii spoken according to claim 1, it is characterised in that in the step (2),
With alcohol steep frutus cnidii three times, merging filtrate, the water of the quality volumes such as frutus cnidii dry weight is added, mix, that is, obtain frutus cnidii
Extract.
3. a kind of method for preparing the bactericide from frutus cnidii, it is characterised in that methods described includes step：
(1) frutus cnidii is dried in 50 DEG C to constant weight, then mechanical crushing is to 40 mesh；
(2) frutus cnidii of above-mentioned crushing is mixed by mass volume ratio 1/2 with ethanol, is then acted on 12 hours in 25 DEG C, filtered,
The water of the quality volumes such as frutus cnidii dry weight is added, is mixed, that is, is obtained common cnidium fruit P.E；
(3) the common cnidium fruit P.E evaporation for obtaining step (2), is concentrated into the quality volume such as frutus cnidii dry weight, adds product total
The organic silicon surfactant of the 1%～3% of weight, the silicone active agent are modified poly- trisiloxanes for ethyoxyl, that is, obtain
Obtain frutus cnidii bactericide.
4. the method that preparation according to claim 3 derives from the bactericide of frutus cnidii, it is characterised in that described
In step (2), with alcohol steep frutus cnidii three times, merging filtrate, the water of the quality volumes such as frutus cnidii dry weight is added, mixed, i.e.,
Obtain common cnidium fruit P.E.
5. the method that preparation according to claim 3 derives from the bactericide of frutus cnidii, it is characterised in that described
In step (3), evaporating temperature is 60 DEG C.
6. the bactericide described in claim 1 from frutus cnidii is used to suppress the application of cotton verticillium wilt pathogen.
7. application according to claim 6, it is characterised in that occur in cotton verticillium wilt phase beginning, dispenser dosage is
200mL/ mu -400mL/ mus, 1 times a week, continuous spraying 3 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610914700.0A CN106538611A (en) | 2016-10-20 | 2016-10-20 | From bactericide its preparation method and the application of frutus cnidii |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610914700.0A CN106538611A (en) | 2016-10-20 | 2016-10-20 | From bactericide its preparation method and the application of frutus cnidii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106538611A true CN106538611A (en) | 2017-03-29 |
Family
ID=58391989
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610914700.0A Pending CN106538611A (en) | 2016-10-20 | 2016-10-20 | From bactericide its preparation method and the application of frutus cnidii |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106538611A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106982859A (en) * | 2017-04-26 | 2017-07-28 | 中国农业科学院植物保护研究所 | A kind of compounded pesticides of Lecanicillium lecanii and Osthole |
| CN110278970A (en) * | 2019-07-31 | 2019-09-27 | 黑龙江省农业科学院植物保护研究所 | A kind of Chinese herbal medicine type seed coat agent and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615705A (en) * | 2004-10-07 | 2005-05-18 | 刘伟平 | Plant source biological pesticide and producing method |
| CN101194626A (en) * | 2006-12-26 | 2008-06-11 | 河南农业大学 | High-efficiency fungicide and method of preparing the same |
| CN101715791A (en) * | 2009-11-27 | 2010-06-02 | 沈阳农业大学 | Preparation for degrading Verticilium dahliae in continuous cropping soil of eggplant, and preparation method thereof |
-
2016
- 2016-10-20 CN CN201610914700.0A patent/CN106538611A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615705A (en) * | 2004-10-07 | 2005-05-18 | 刘伟平 | Plant source biological pesticide and producing method |
| CN101194626A (en) * | 2006-12-26 | 2008-06-11 | 河南农业大学 | High-efficiency fungicide and method of preparing the same |
| CN101715791A (en) * | 2009-11-27 | 2010-06-02 | 沈阳农业大学 | Preparation for degrading Verticilium dahliae in continuous cropping soil of eggplant, and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 朱荷琴等: "22种中药提取物对棉花黄萎病菌的抑菌活性", 《棉花学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106982859A (en) * | 2017-04-26 | 2017-07-28 | 中国农业科学院植物保护研究所 | A kind of compounded pesticides of Lecanicillium lecanii and Osthole |
| CN110278970A (en) * | 2019-07-31 | 2019-09-27 | 黑龙江省农业科学院植物保护研究所 | A kind of Chinese herbal medicine type seed coat agent and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mkindi et al. | Invasive weeds with pesticidal properties as potential new crops | |
| Babu Joseph et al. | Bioefficacy of plant extracts to control Fusarium solani f. sp. melongenae incitant of brinjal wilt | |
| Tanveer et al. | Interference and management of parthenium: the world's most important invasive weed | |
| CN100426963C (en) | Use of metabolite of eupatorium in biologically herbicidal | |
| Juber et al. | Fusarium wilt disease of strawbrry caused by Fusarium oxysporum f. sp. fragariae in Iraq and its control. | |
| CN102217661B (en) | Botanical bactericide and preparation method and application thereof | |
| CN104938525A (en) | Preparation method and application of plant fungicide | |
| Maity et al. | Management of chilli thrips with some newer molecules of chemicals | |
| Naz et al. | Sustainable management of the southern root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood, by means of amendments of Fumaria parviflora. | |
| Jabeen et al. | Interactive activity of Asphodelus tenuifolius on germination and growth of wheat (Triticum aestivium L.) and sorghum (Sorghum bicolor L.) | |
| Cordeiro et al. | Mycorrhization stimulant based in formononetin associated to fungicide and doses of phosphorus in soybean in the Cerrado | |
| CN106538611A (en) | From bactericide its preparation method and the application of frutus cnidii | |
| Alao et al. | Preliminary evaluation of the insecticidal potential of organic compost extracts against insect pests of Okra (Abelmoschus esculentus (L.) Moench) | |
| Moreno | Crop protection implications of cassava intercropping | |
| CN106070334A (en) | The preparation method of Galla Chinensis source pesticide and the application in preventing and treating cotton verticillium wilt thereof | |
| Ogah | Field evaluation of plant extracts in the management of Megalurothrips sjostedti and Maruca vitrata of cowpea in southeastern Nigeria | |
| Sathua et al. | Bio-efficacy of various insecticides and botanicals against chilli thrips (S. dorsalis Hood) and their comparative cost: Benefit analysis in chilli crop | |
| CN102204576A (en) | Method for relieving damage of hirschmanniella oryzae to rice by using Agrimonia pilosa Ledeb. extract | |
| Ramos et al. | Neem (Azadirachta indica a. Juss) components: candidates for the control of Crinipellis perniciosa and Phytophtora spp. | |
| Taupik et al. | Allelopathic potential of cassava (Manihot esculenta L.) extracts on germination and seedling growth of selected weeds and aerobic rice | |
| CN106561725A (en) | Asarum-source botanical bactericide and preparation method and application thereof | |
| Santana Baños et al. | Biostimulant and Nematicidal Effect of Trichoderma harzianum Rifai and Aqueous Extract of Azadirachta indica A. Juss. in Solanum lycopersicum L. | |
| CN106561727B (en) | From the bactericide preparation method of tripterygium wilfordii and application | |
| Abu Irmaileh et al. | Antifungal activity of the stinkwort (Inula graveolens) extracts | |
| Odewole et al. | Comparative efficacy of plant extracts with synthetic against insect pests of okra (Abelmoschus esculentus L. Monech) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170329 |
|
| RJ01 | Rejection of invention patent application after publication |