CN110278970A - A kind of Chinese herbal medicine type seed coat agent and preparation method thereof - Google Patents
A kind of Chinese herbal medicine type seed coat agent and preparation method thereof Download PDFInfo
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- CN110278970A CN110278970A CN201910701204.0A CN201910701204A CN110278970A CN 110278970 A CN110278970 A CN 110278970A CN 201910701204 A CN201910701204 A CN 201910701204A CN 110278970 A CN110278970 A CN 110278970A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
Abstract
A kind of Chinese herbal medicine type seed coat agent and preparation method thereof, belongs to seed coat agent preparation technical field.The technical issues of in order to solve root rot prevention and treatment, the present invention provides a kind of Chinese herbal medicine type seed coat agents, it is by by the raw material of following weight percent: common cnidium fruit P.E 20%-30%, anemarrhena asphodefoides extract 15%-25%, wetting agent 0.4%-0.5%, dispersing agent 2.0%, thickener 2.5%, film former 1.0%, antifreezing agent 5%, surplus is water, size mixing after mixing be machined to partial size be 35-40 μm prepare.Seed coat agent prepared by the present invention has significant inhibiting effect for Fusarium oxysporum, it can inhibit soybean and other crop root rots occur, reduce and substitute traditional chemical pesticide and use, reduce cost, pollution of the chemical pesticide to environment is reduced, the disease incidence of root rot is significantly reduced.
Description
Technical field
The invention belongs to seed coat agent preparation technical fields, and in particular to a kind of Chinese herbal medicine type seed coat agent and preparation method thereof.
Background technique
Root rot is kind of a normal hair venereal disease evil, and the country is mainly distributed on northeast soybean producing region and Huang-Huai Area.The disease with
Native biography mode causes to seriously endanger to soybean seedling, generally can the underproduction 10%~30%, when weight calamity can 60% or more the underproduction even
Total crop failure.Root rot Species of Pathogens is more and prevention and treatment is difficult, and reclamation area in Heilongjiang Province is using Fusarium oxysporum as dominant bacteria, furthermore
Fusarium oxysporum can cause the root rot of the various crops such as apple, grape, capsicum, cucurbita pepo, alfalfa and Chinese herbal medicine, suppression
Metal pointing fusarium oxysporum will all be of great significance to various crop root rots such as prevention and treatment soybean.
Prevention and treatment root rot is mainly sterilized by chemical reagent at present, and not only cost of material is high, but also to human body and soil
Earth environment can all affect greatly, and also not be suitable for green organic food.Although preventing and treating economical and effective using disease-resistant variety,
Single resistant variety, which is used for a long time, is easy to cause soybean to lose the resistance of root rot；The biological seed coating agent researched and developed is not only right
Root rot preventive effect caused by Fusarium oxysporum is poor, also incomplete in extraction and manufacture craft, therefore develops environment friend
Good type green prevention and control technology is for disease suppression, and ensures that crop safety is all of great significance.
Summary of the invention
It is the technical issues of in order to solve root rot prevention and treatment, described the present invention provides a kind of Chinese herbal medicine type seed coat agent
Seed coat agent is prepared by the raw material of following weight percent: common cnidium fruit P.E 20%-30%, anemarrhena asphodefoides extract 15%-
25%, wetting agent 0.4%-0.5%, dispersing agent 2.0%, thickener 2.5%, film former 1.0%, antifreezing agent 5.0%, surplus are
Water.
Preferably, the seed coat agent is prepared by the raw material of following weight percent: common cnidium fruit P.E 20%, rhizoma anemarrhenae
Extract 25%, wetting agent 0.5%, dispersing agent 2.0%, thickener 2.5%, film former 1.0%, antifreezing agent 5.0%, surplus are
Water.
It further limits, the wetting agent is one of wetting agent CL-3, JFC and dodecyl sodium sulfate or a variety of
Mixture；The dispersing agent is the mixture of one or both of dispersing agent 602 and AEO-3P；The thickener is silicic acid
The mixture of one or both of magnalium and xanthan gum；The film former is sodium carboxymethylcellulose and polyvinylpyrrolidone
One or both of mixture；The antifreezing agent is the mixture of one or both of ethylene glycol and glycerine.
The present invention also provides the preparation methods of said herbal medicine type seed coat agent: preparing common cnidium fruit P.E and rhizoma anemarrhenae respectively
Extract, then by common cnidium fruit P.E, anemarrhena asphodefoides extract and wetting agent, dispersing agent, thickener, film former, antifreezing agent and water
It sizes mixing after mixing by weight percentage, it is 35-40 μm that slurry, which is machined to partial size, obtains finished product.
Further limit, the common cnidium fruit P.E the preparation method is as follows: by frutus cnidii through 50-60 DEG C dry, powder
Sieving after broken, obtained Common Cnidium Fruit, the ethanol solution for being 95% with the concentration (volume) of 5 times of volumes, 35-55 DEG C is extracted 3
It is secondary, it is 1-3 hours each, merge ethanol extract, is concentrated and recycles ethyl alcohol, obtain common cnidium fruit P.E.
It further limits, after the frutus cnidii crushes, crosses 20 meshes.
Further limit, the anemarrhena asphodefoides extract the preparation method is as follows: by rhizoma anemarrhenae through 50-60 DEG C dry, after crushing
Sieving, obtained Common Anemarrhena Rhizome, the ethanol solution for being 95% with the concentration (volume) of 5 times of volumes, 45 DEG C are extracted 3 times, and 2 is small every time
When, merge ethanol extract, is concentrated and recycles ethyl alcohol, obtain anemarrhena asphodefoides extract.
It further limits, after the rhizoma anemarrhenae crushes, crosses 20 meshes.
Beneficial effect
The invention has the advantages that having selected common cnidium fruit P.E and anemarrhena asphodefoides extract both excellent inhibitor, kind ensure that
Fungistatic effect possessed by clothing.Capsuled seed through seed coat agent preparation has good vitality and germination percentage, for sharp spore sickle
Knife bacterium has significant inhibiting effect, can prevent and treat soybean and other crop root rots, reducing and substitute traditional chemical pesticide makes
With reducing cost, reduce pollution of the chemical pesticide to environment, significantly reduce the disease incidence of root rot.
Specific embodiment
The present invention is described in detail below in conjunction with specific embodiment.
The preparation method of 1. Chinese herbal medicine type seed coat agent of embodiment.
A kind of Chinese herbal medicine type seed coat agent is present embodiments provided, is prepared by the raw material of following weight percent: cnidium monnieri
Seed extract 20%, anemarrhena asphodefoides extract 25%, wetting agent CL-3 0.5%, dispersing agent 602 2.0%, aluminium-magnesium silicate 0.5%, Huang
Virgin rubber 2.0%, film former sodium carboxymethylcellulose 1.0%, antifreeze glycol 5%, surplus are water.Specific preparation method is such as
Under:
1. the preparation of common cnidium fruit P.E:
It takes appropriate Fructus cnidii to dry in 60 DEG C, crushed 20 meshes, be 95% with the concentration (volume) of 5 times of volumes
Ethanol solution shaken in 55 DEG C of water-baths extraction 3 times, 3 hours every time, merge ethanol extract, be concentrated and recycle ethyl alcohol, obtain
To common cnidium fruit P.E.
2. the preparation of anemarrhena asphodefoides extract:
60 DEG C of appropriate Fructus cnidii drying are taken, crushed 20 meshes, are 95% with the concentration (volume) of 5 times of volumes
45 DEG C of water-bath concussions of ethyl alcohol are extracted 3 times, 2 hours every time, are merged ethanol extract, are concentrated and recycle ethyl alcohol, obtain rhizoma anemarrhenae extraction
Object.
3. the preparation of Chinese herbal medicine type coating agent for seed: knowing prepared by the common cnidium fruit P.E of step 1) preparation, step 2)
Female extract is sized mixing after mixing by weight percentage with wetting agent, dispersing agent, thickener, film former, antifreezing agent and water, is cut with height
Cut Mixingemulsificationmachine it is pre-dispersed after, slurry poured into sand mill be machined to material particular diameter distribution and reach 35 μm, using 35 μm of filter membranes,
Finished product, appearance light green is filtered by vacuum to obtain.
The preparation method of 2. Chinese herbal medicine type seed coat agent of embodiment.
A kind of Chinese herbal medicine type seed coat agent is present embodiments provided, is prepared by the raw material of following weight percent: cnidium monnieri
Seed extract 20%, anemarrhena asphodefoides extract 15%, wetting agent jfc 0.4%, dispersant A EO-3P2.0%, aluminium-magnesium silicate 0.5%, Huang
Virgin rubber 2.0%, film former polyvinylpyrrolidone 1.0%, antifreezing agent glycerine 5%, surplus are water.Specific preparation method is such as
Under:
1. the preparation of common cnidium fruit P.E:
It takes appropriate Fructus cnidii to dry in 50 DEG C, crushed 20 meshes, be 95% with the concentration (volume) of 4 times of volumes
Ethanol solution shaken in 45 DEG C of water-baths extraction 3 times, 2 hours every time, merge ethanol extract, be concentrated and recycle ethyl alcohol, obtain
To common cnidium fruit P.E.
2. the extraction purification of rhizoma anemarrhenae:
55 DEG C of appropriate Fructus cnidii drying are taken, crushed 20 meshes, are 95% with the concentration (volume) of 4 times of volumes
45 DEG C of water-bath concussions of ethyl alcohol are extracted 3 times, 2 hours every time, are merged ethanol extract, are concentrated and recycle ethyl alcohol, obtain rhizoma anemarrhenae extraction
Object.
3. the preparation of Chinese herbal medicine type coating agent for seed: knowing prepared by the common cnidium fruit P.E of step 1) preparation, step 2)
Female extract is sized mixing after mixing by weight percentage with wetting agent, dispersing agent, thickener, film former, antifreezing agent and water, is cut with height
Cut Mixingemulsificationmachine it is pre-dispersed after, slurry poured into sand mill be machined to material particular diameter distribution and reach 35 μm, using 35 μm of filter membranes,
Finished product, appearance light green is filtered by vacuum to obtain.
The preparation method of 3. Chinese herbal medicine type seed coat agent of embodiment.
A kind of Chinese herbal medicine type seed coat agent is present embodiments provided, is prepared by the raw material of following weight percent: cnidium monnieri
Seed extract 30%, anemarrhena asphodefoides extract 25%, wetting agent dodecyl sodium sulfate 0.4%, dispersant A EO-3P2.0%, silicic acid
Magnalium 0.5%, xanthan gum 2.0%, film former polyvinylpyrrolidone 1.0%, antifreeze glycol 5%, surplus are water.Specifically
The preparation method is as follows:
1. the preparation of common cnidium fruit P.E:
It takes appropriate Fructus cnidii to dry in 55 DEG C, crushed 20 meshes, be 95% with the concentration (volume) of 5 times of volumes
Ethanol solution shaken in 50 DEG C of water-baths extraction 3 times, 3 hours every time, merge ethanol extract, be concentrated and recycle ethyl alcohol, obtain
To ethanol extract.
2. the extraction purification of rhizoma anemarrhenae:
55 DEG C of appropriate Fructus cnidii drying are taken, crushed 20 meshes, are 95% with the concentration (volume) of 5 times of volumes
45 DEG C of water-bath concussions of ethyl alcohol are extracted 3 times, 2 hours every time, are merged ethanol extract, are concentrated and recycle ethyl alcohol, obtain rhizoma anemarrhenae extraction
Object.
3. the preparation of Chinese herbal medicine type coating agent for seed: knowing prepared by the common cnidium fruit P.E of step 1) preparation, step 2)
Female extract is sized mixing after mixing by weight percentage with wetting agent, dispersing agent, thickener, film former, antifreezing agent and water, is cut with height
Cut Mixingemulsificationmachine it is pre-dispersed after, slurry poured into sand mill be machined to material particular diameter distribution and reach 35 μm, using 35 μm of filter membranes,
Finished product, appearance light green is filtered by vacuum to obtain.
According to GB/T 17768-1999 detect the suspensibility of seed coat agent of the method for the present invention preparation, viscosity, film formation time,
Coating uniformity, coating expulsion rate, performance indicator is as follows after seed coat agent is made:
The physical and chemical index of the Chinese herbal medicine type seed coat agent prepared by the present invention of table 1
| Index name | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Suspensibility (%) | 99.46 | 94.35 | 96.38 |
| 25 DEG C of viscosity (mPaS) | 750.00 | 720.00 | 740.00 |
| PH value | 4.56 | 5.23 | 5.09 |
| Film formation time (min) | 15.38 | 17.92 | 16.84 |
| Coating uniformity (%) | 98.00 | 92.00 | 96.00 |
| It is coated expulsion rate (%) | 0.2 | 0.8 | 0.6 |
Influence of the biological coating agent of embodiment 4. to soybean germination gesture and germination percentage
The present embodiment investigates Chinese herbal medicine type seed coat agent prepared by the present invention to big by taking seed coat agent prepared by embodiment 1 as an example
The influence of beans germination.
By selected 51 soya seeds of black agriculture, 41 DEG C of constant temperature impregnate 4h, and then at bored kind of 8h of 25 DEG C of moisturizings, centre is changed for several times
Water, after with blotting paper to be drawn to surface anhydrous, air-dry 30min at room temperature, then with clothing weight: the weight ratio of Zhong Chongwei 1:50 takes kind
Sub- coating agent is coated, and is control with not capsuled seed, and selection 100 is set in the culture dish for having spread the double-deck wet filter paper and spread out
It opens, above covers wet filter paper, be repeated 3 times, and be put into 25 DEG C of constant incubators, observation in the 4th day counts germinating energy, observes within the 7th day
Count germination percentage.
Germination test is carried out indoors, is control with not capsuled seed, and every kind is repeated 4 times, and every repetition 100 is put
It in culture dish, sets in biochemical cultivation case and germinates, germination temperature is 25 DEG C.Record chitting piece number daily, 4d play meter
Calculate germinating energy, for 10d with " bud length=0.5 kind long ", " root long=kind long " is criterion calculation germination percentage.Same day grab sample 20
Strain measures height of seedling, longest root long with ruler.With " root long=kind long " for the primary branch radical of criterion calculation, with a ten thousandth electronics
Balance measures fresh dry matter, and drainage measurement root system volume, fresh dry method of double differences seek fresh weight water content.According to daily germination kind
Subnumber calculates germination index (GI), germination value (GV), average germinating time (MGT).
Germination value GV=peak value × average germination percentage；
Germination index GI=Σ (Gt/Dt)；
Average germinating time MGT=[Σ (Gt × Dt)]/Σ Gt,
Chitting piece number in Gt:t days, Dt: germination number of days.
Soybean germination rate application form after the biological Cotton seeds of table 2
By data it is found that the soya seeds mean germination gesture and average germination percentage of coating are higher by 3.22% He than control
0.51%, this biology coating agent can promote the sprouting of soya seeds.
Control efficiency of the biological coating agent of embodiment 5. to root rot.
The present embodiment investigates Chinese herbal medicine type seed coat agent prepared by the present invention to big by taking seed coat agent prepared by embodiment 1 as an example
The control efficiency of beans root rot.
Main diseases and insect pests experimental investigation: test is in Heilongjiang Institute of Agricultural Sciences's (126 ° 63 ' of east longitude, 45 ° 75 ' of north latitude)
It is carried out in garden, it is control with blank seed that it is processing with seed coat agent coating that experimental field smooth, fertility is uniform.After coating
It is sowed, line-spacing 45cm, spacing in the rows 10cm, the same crop field of field management.It tests in the state of an illness of soybean growth period investigation soybean root rot
Index (diagonal line bikini sampling, amount to 100 plants), while (3 points of diagonal line of aphid occurrence degree are investigated when aphid occurs
Formula investigation, amounts to 40 plants, is converted into 100 plants).For the grade scale of root rot referring to Xin Huipu, Ma Huiquan, Liu Jingru, et al. is big
Primary Study [J] Soybean Science with prevention and treatment occurs for beans root rot, and the method for " material and method " slightly changes in 1987 (3)
It is dynamic, specific as follows:
0 grade: subterranean stem and root disease-free spot；
1 grade: subterranean stem and root have fragmentary scab, but not in flakes；
2 grades: subterranean stem and root have more scab, and lesion area accounts for 1/4 or less stem and the root gross area；
3 grades: subterranean stem and root have volume scab, and lesion area accounts for 1/4 or more of stem and the root gross area, less than 1/2 seedling
Growth retardation；
4 grades: subterranean stem and root lesion area account for the l/2 or more of stem and the root gross area, less than 3/4；
5 grades: subterranean stem and root lesion area account for 3/4 or more of stem and the root gross area, stem and butt rot.
3 root rot of table a situation arises application form
| Processing | Disease index | Control efficiency (%) |
| Control | 33.25 | —— |
| Coating | 13.25 | 60.15 |
According to test it is found that the morbidity for significantly reducing root rot of the soybean after being coated using this biological seed coating agent
Rate, control efficiency can reduce root rot up to 60.15%, using the biological coating agent of Chinese medicine ingredient to a certain extent
Disease index improves the resistance of soybean.
Claims (8)
1. a kind of Chinese herbal medicine type seed coat agent, which is characterized in that the seed coat agent is prepared by the raw material of following weight percent:
Common cnidium fruit P.E 20%-30%, anemarrhena asphodefoides extract 15%-25%, wetting agent 0.4%-0.5%, dispersing agent 2.0%, thickening
Agent 2.5%, film former 1.0%, antifreezing agent 5.0%, surplus are water.
2. Chinese herbal medicine type seed coat agent according to claim 1, which is characterized in that the seed coat agent is by following weight percent
Raw material prepare: common cnidium fruit P.E 20%, anemarrhena asphodefoides extract 25%, wetting agent 0.5%, dispersing agent 2.0%, thickener
2.5%, film former 1.0%, antifreezing agent 5.0%, surplus are water.
3. Chinese herbal medicine type seed coat agent according to claim 1, which is characterized in that the wetting agent is wetting agent CL-3, JFC
With one of dodecyl sodium sulfate or a variety of mixtures；The dispersing agent is one of dispersing agent 602 and AEO-3P
Or two kinds of mixture；The thickener is the mixture of one or both of aluminium-magnesium silicate and xanthan gum；The film former
For the mixture of one or both of sodium carboxymethylcellulose and polyvinylpyrrolidone；The antifreezing agent is ethylene glycol and third
The mixture of one or both of triol.
4. the preparation method of Chinese herbal medicine type seed coat agent described in claim 1-3 any one, which is characterized in that prepare snake respectively
Machine tool extract and anemarrhena asphodefoides extract, then by common cnidium fruit P.E, anemarrhena asphodefoides extract and wetting agent, dispersing agent, thickener, at
Mould agent, antifreezing agent and water are sized mixing after mixing by weight percentage, and it is 35-40 μm that slurry, which is machined to partial size, obtain finished product.
5. the preparation method according to claim 4, which is characterized in that the common cnidium fruit P.E the preparation method is as follows:
Frutus cnidii is dried through 50-60 DEG C, grinding and sieving, obtained Common Cnidium Fruit, the ethyl alcohol for being 95% with the concentration of 5 times of volumes
Solution, 35-55 DEG C is extracted 3 times, 1-3 hours each, is merged ethanol extract, is concentrated and recycles ethyl alcohol, and frutus cnidii extraction is obtained
Object.
6. preparation method according to claim 5, which is characterized in that after the frutus cnidii crushes, cross 20 meshes.
7. the preparation method according to claim 4, which is characterized in that the anemarrhena asphodefoides extract the preparation method is as follows: will
Rhizoma anemarrhenae is dried through 50-60 DEG C, grinding and sieving, obtained Common Anemarrhena Rhizome, the ethanol solution for being 95% with the concentration of 5 times of volumes,
45 DEG C are extracted 3 times, 2 hours every time, are merged ethanol extract, are concentrated and recycle ethyl alcohol, obtain anemarrhena asphodefoides extract.
8. preparation method according to claim 7, which is characterized in that after the rhizoma anemarrhenae crushes, cross 20 meshes.
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| CN115119847A (en) * | 2022-06-28 | 2022-09-30 | 黑龙江省农业科学院植物保护研究所 | Chelerythrine wettable powder and preparation method and application thereof |
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