CN106417284A - Biological bactericide for preventing and treating apple tree canker and preparation method thereof - Google Patents
Biological bactericide for preventing and treating apple tree canker and preparation method thereof Download PDFInfo
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- CN106417284A CN106417284A CN201610928146.1A CN201610928146A CN106417284A CN 106417284 A CN106417284 A CN 106417284A CN 201610928146 A CN201610928146 A CN 201610928146A CN 106417284 A CN106417284 A CN 106417284A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a biological bactericide for preventing and treating apple tree canker and a preparation method thereof. The biological bactericide for preventing and treating apple tree canker is prepared from the following raw materials in parts by weight: 5-10 parts of anemone, 8-12 parts of Mangnolia officinalis, 7-15 parts of Xanthium strumarium, 8-15 parts of rhizoma anemarrhenae, 10-20 parts of mulberry bark, 5-10 parts of fructus cnidii, 4-8 parts of Chinaberry seed, 10-20 parts of dispersing agent, 3-10 parts of wetting agent, 0-5 parts of disintegrant and 40-60 parts of filler. The biological bactericide for preventing and treating apple tree canker has the advantages of stable drug properties, long preservation period (up to 2 years), no residue, high degradability and no harm to the human and livestock, and can be popularized and applied.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of biofungicide for preventing and treating apple tree canker and its system
Preparation Method.
Background technology
Apple tree canker, also known as bark rot, smelly skin disease, is that each Main Apple Varieties producing region of China generally occurs and endangers serious
A kind of disease.Apple tree canker is generally present in the trunk of apple tree, major branch and cuts according to mouth, when disease occurs lighter, draws
Play the rotting of Fructus Mali pumilae bark, tree vigo(u)r is weak, apple production and quality have declined, and causes branch to dry up, whole tree when disease is serious
Withered, or even cause to ruin garden.The extensive application of traditional chemical pesticide, while economic benefit is brought, also brings
The pollution of a series of negative effect, such as chemical pesticide, residue problem, as the low toxicity of botanical pesticide, selectivity are high, easily
The advantages of degraded, plant are not likely to produce resistance to which, the research and development extremely concern of people and the favor of botanical fungicide in recent years.
Chinese patent CN104430626A discloses a kind of botanical pesticide for preventing and treating apple tree canker, belongs to fruit tree disease
Pest control technology field, the raw material of the pesticide includes Pericarpium Zanthoxyli, Bulbus Allii, Aloe, Flos Caryophylli, Radix Isatidis, antifreezing agent, Wound protection
Agent, preservative.But, the patent effective ingredient is volatile oil, should not to keep drug effect for a long time, readily volatilized.
Chinese patent CN102812972A discloses a kind of botanical pesticide, and more particularly to a kind of preventing and treating apple tree is rotted
The plant extract combination of disease and preparation method, the existing medicine material for preventing and treating apple tree canker of solution is single, and effect is paid no attention to
The problems such as thinking, by 0.5-1.5 part Herba Sophorae alopecuroidiss Herb, 1-3 part Radix Sophorae Flavescentiss fruit, 0.5-1.5 part Fructus Toosendan fruit, 0.3-0.7 part Radix Euphorbiae Pekinensis
Chameajasme, 0.2-0.4 part Nicotiana tabacum L. composition.But, above-mentioned patent raw material is mainly fruit class, and effective component extraction rate is low.
Content of the invention
For overcoming drawbacks described above, it is an object of the invention to provide a kind of biofungicide for preventing and treating apple tree canker and
Its preparation method.
For achieving the above object, the present invention is adopted the following technical scheme that：
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
Radix Pulsatillae 5-10 part, Cortex Magnoliae Officinalis 8-12 part, Herba Xanthii 7-15 part, Rhizoma Anemarrhenae 8-15 part, Cortex Mori 10-20 part, Fructus Cnidii 5-10 part,
Fructus Toosendan 4-8 part, dispersant 10-20 part, wetting agent 3-10 part, disintegrating agent 0-5 part and filler 40-60 part.
Preferably, described dispersant is sodium lignin sulfonate, calcium lignosulfonate, oleic acid polyoxyethylene, dodecyl
At least one of sodium sulfate and dodecylbenzene sodium sulfonate.
Preferably, the wetting agent be di-2-ethylhexylphosphine oxide how in sodium sulfonate, naphthalene sulfonic acid-formaldehyde condensation product and methyl oleate at least
A kind of.
Preferably, the disintegrating agent is at least one of ammonium sulfate, carbamide and low-substituted hydroxypropyl cellulose.
Preferably, described filler is at least one in Kaolin, bentonite, kieselguhr and attapulgite clay.
A kind of preparation method of the biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）The alcohol solution dipping 10-20h of 5-10 times of weight is added in compound, then under the conditions of 50-60 DEG C
Supersound extraction 2-3 time, each 20-40min；
（3）By step（2）Middle gained extracting solution is evaporated to the 10-20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 20-30min is stirred, through comminution by gas stream,
20-30min is again stirring for, is obtained final product.
Preferably, step（1）The plant material is crushed to particle diameter for 40-80 mesh.
Preferably, step（2）Volumes of aqueous ethanol concentration >=60%.
Preferably, step（2）Supersonic frequency 20-30kHz.
Preferably, step（4）The comminution by gas stream to powder diameter is 400-600 mesh.
The positive beneficial effect of the present invention：
1. Radix Pulsatillae hardship of the present invention, trembles with fear, heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, removing dampness and killing parasites；Cortex Magnoliae Officinalis hardship, pungent, warm in nature, antibacterial；Herba Xanthii
Toil, trembles with fear, wind and heat eliminating, and detoxify parasite killing；The Rhizoma Anemarrhenae is bitter, sweet, trembles with fear, and resisting pathogenic microbes act on；Cortex Mori is sweet, trembles with fear, antibacterial；Above-mentioned
The Radix Pulsatillae, Cortex Magnoliae Officinalis, Herba Xanthii, the Rhizoma Anemarrhenae and Cortex Mori are shared, and the property of medicine is cold and cool, and fungistatic effect is notable.Fructus Cnidii is pungent, bitter, temperature, and the kidney warming is strengthened
Sun, dampness, wind-dispelling, parasite killing；Fructus Toosendan hardship, trembles with fear, qi-regulating, pain relieving, clearing away damp-heat, anthelmintic；Above-mentioned Fructus Cnidii, Fructus Toosendan auxiliary hoary hair
Father-in-law, Cortex Magnoliae Officinalis, Herba Xanthii, the Rhizoma Anemarrhenae and Cortex Mori effect, enhance the function of antibacterial, and above-mentioned each plant material and adjuvant prepare wettable
Property powder, the property of medicine is stable, is kept for the phase up to 2 years, noresidue, degradable, to person poultry harmless, can be applicable.
2. dispersant of the present invention is sodium lignin sulfonate, calcium lignosulfonate, oleic acid polyoxyethylene, dodecane
At least one of base sodium sulfate and dodecylbenzene sodium sulfonate, biological degradation rate height, with low cost.The wetting agent is methylene
At least one of double how sodium sulfonates, naphthalene sulfonic acid-formaldehyde condensation product and methyl oleate, are sufficiently mixed uniformly to each raw material.The disintegrate
Agent is at least one of ammonium sulfate, carbamide and low-substituted hydroxypropyl cellulose, and wettable powder rapid dispersion in water is easy to spray
Spill.Described filler is at least one in Kaolin, bentonite, kieselguhr and attapulgite clay, and filler aperture is big, equal to raw material
Even dispersion, is conducive to giving full play to for drug effect.
3. each plant material extracts active ingredients of the present invention are complete, and effective ingredient is not volatile, efficacy stability, low cost
Honest and clean.
Specific embodiment
With reference to some specific embodiments, the present invention is further described.
Embodiment 1
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
5 parts of the Radix Pulsatillae, 8 parts of Cortex Magnoliae Officinalis, 7 parts of Herba Xanthii, 8 parts of the Rhizoma Anemarrhenae, 10 parts of Cortex Mori, 5 parts of Fructus Cnidii, 4 parts of Fructus Toosendan, dispersant 10
Part, 3 parts of wetting agent and 40 parts of filler.
The dispersant is sodium lignin sulfonate.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate.
Described filler is Kaolin.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 40 mesh, mix homogeneously；
（2）By step（1）75% alcohol solution dipping 10h of 5 times of weight is added in compound, and then ultrasound is carried under the conditions of 50 DEG C
Take 2 times, each 20min；Supersonic frequency 20kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 10% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent and filler are added in plant extract, 20min is stirred, through comminution by gas stream to 400 mesh, again
Stirring 20min, obtains final product.
Embodiment 2
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
6 parts of the Radix Pulsatillae, 9 parts of Cortex Magnoliae Officinalis, 8 parts of Herba Xanthii, 9 parts of the Rhizoma Anemarrhenae, 12 parts of Cortex Mori, 6 parts of Fructus Cnidii, 5 parts of Fructus Toosendan, dispersant 11
Part, 4 parts of wetting agent, 1 part of disintegrating agent and 40 parts of filler.
The dispersant is sodium lignin sulfonate.
The wetting agent is methyl oleate.
The disintegrating agent is carbamide.
Described filler is Kaolin.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 50 mesh, mix homogeneously；
（2）By step（1）60% alcohol solution dipping 15h of 6 times of weight is added in compound, and then ultrasound is carried under the conditions of 55 DEG C
Take 3 times, each 20min；Supersonic frequency 30kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 15% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 500
Mesh, is again stirring for 30min, obtains final product.
Embodiment 3
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
7 parts of the Radix Pulsatillae, 9.5 parts of Cortex Magnoliae Officinalis, 9 parts of Herba Xanthii, 10 parts of the Rhizoma Anemarrhenae, 13 parts of Cortex Mori, 7 parts of Fructus Cnidii, 6 parts of Fructus Toosendan, dispersion
13 parts of agent, 5 parts of wetting agent, 2 parts of disintegrating agent and 50 parts of filler.
The dispersant is calcium lignosulfonate.
The wetting agent is naphthalene sulfonic acid-formaldehyde condensation product.
The disintegrating agent is ammonium sulfate and carbamide, and both weight ratios are 1：1.
Described filler is bentonite.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 60 mesh, mix homogeneously；
（2）By step（1）80% alcohol solution dipping 20h of 8 times of weight is added in compound, and then ultrasound is carried under the conditions of 60 DEG C
Take 3 times, each 30min；Supersonic frequency 25kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 600
Mesh, is again stirring for 25min, obtains final product.
Embodiment 4
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
8 parts of the Radix Pulsatillae, 10 parts of Cortex Magnoliae Officinalis, 10 parts of Herba Xanthii, 11 parts of the Rhizoma Anemarrhenae, 15 parts of Cortex Mori, 7.5 parts of Fructus Cnidii, 6.5 parts of Fructus Toosendan,
15 parts of dispersant, 6 parts of wetting agent, 3 parts of disintegrating agent and 50 parts of filler.
The dispersant is sodium lauryl sulphate.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate.
The disintegrating agent is ammonium sulfate.
Described filler is attapulgite clay.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 60 mesh, mix homogeneously；
（2）By step（1）80% alcohol solution dipping 15h of 8 times of weight is added in compound, and then ultrasound is carried under the conditions of 55 DEG C
Take 3 times, each 30min；Supersonic frequency 30kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 15% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 500
Mesh, is again stirring for 30min, obtains final product.
Embodiment 5
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
8 parts of the Radix Pulsatillae, 10 parts of Cortex Magnoliae Officinalis, 10 parts of Herba Xanthii, 11 parts of the Rhizoma Anemarrhenae, 15 parts of Cortex Mori, 7.5 parts of Fructus Cnidii, 6.5 parts of Fructus Toosendan,
8 parts of Herba Portulacae, 15 parts of dispersant, 6 parts of wetting agent, 3 parts of disintegrating agent and 50 parts of filler.
The present embodiment raw material adds Herba Portulacae, and Herba Portulacae acid is cold, and heat-clearing and toxic substances removing, diuretic damp eliminating, scattered blood detumescence, parasite killing go out
Bacterium, is shared with various raw materials, enhances the fungistatic effect of antibacterial.
The dispersant is sodium lauryl sulphate.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate.
The disintegrating agent is ammonium sulfate.
Described filler is attapulgite clay.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker is shown in embodiment 4.
Embodiment 6
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
8.5 parts of the Radix Pulsatillae, 11 parts of Cortex Magnoliae Officinalis, 11 parts of Herba Xanthii, 12 parts of the Rhizoma Anemarrhenae, 16 parts of Cortex Mori, 8 parts of Fructus Cnidii, 7 parts of Fructus Toosendan, point
16 parts of powder, 7 parts of wetting agent, 4 parts of disintegrating agent and 55 parts of filler.
The dispersant is dodecylbenzene sodium sulfonate.
The wetting agent is methyl oleate.
The disintegrating agent is low-substituted hydroxypropyl cellulose.
Described filler is attapulgite clay and kieselguhr, and both weight ratios are 1:1.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 80 mesh, mix homogeneously；
（2）By step（1）95% alcohol solution dipping 20h of 10 times of weight is added in compound, then ultrasound under the conditions of 50 DEG C
Extract 2 times, each 40min；Supersonic frequency 20kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 20min is stirred, through comminution by gas stream to 600
Mesh, is again stirring for 25min, obtains final product.
Embodiment 7
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
9 parts of the Radix Pulsatillae, 11.5 parts of Cortex Magnoliae Officinalis, 13 parts of Herba Xanthii, 14 parts of the Rhizoma Anemarrhenae, 18 parts of Cortex Mori, 9 parts of Fructus Cnidii, 8 parts of Fructus Toosendan, point
18 parts of powder, 8 parts of wetting agent, 4.5 parts of disintegrating agent and 60 parts of filler.
The dispersant is oleic acid polyoxyethylene.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate and methyl oleate, both weight ratios be:1.
The disintegrating agent is low-substituted hydroxypropyl cellulose.
Described filler is bentonite.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 60 mesh, mix homogeneously；
（2）By step（1）90% alcohol solution dipping 15h of 8 times of weight is added in compound, and then ultrasound is carried under the conditions of 50 DEG C
Take 3 times, each 30min；Supersonic frequency 25kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 15% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 25min is stirred, through comminution by gas stream to 500
Mesh, is again stirring for 30min, obtains final product.
Embodiment 8
A kind of biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
10 parts of the Radix Pulsatillae, 12 parts of Cortex Magnoliae Officinalis, 15 parts of Herba Xanthii, 15 parts of the Rhizoma Anemarrhenae, 20 parts of Cortex Mori, 10 parts of Fructus Cnidii, 8 parts of Fructus Toosendan, point
20 parts of powder, 10 parts of wetting agent, 5 parts of disintegrating agent and 60 parts of filler.
The dispersant is sodium lauryl sulphate and oleic acid polyoxyethylene, and both weight ratios are 1：1.
The wetting agent is naphthalene sulfonic acid-formaldehyde condensation product.
The disintegrating agent is carbamide.
Described filler is kieselguhr.
The preparation method of the above-mentioned biofungicide for preventing and treating apple tree canker, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 60 mesh, mix homogeneously；
（2）By step（1）75% alcohol solution dipping 20h of 10 times of weight is added in compound, then ultrasound under the conditions of 55 DEG C
Extract 2 times, each 20min；Supersonic frequency 30kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 10% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 600
Mesh, is again stirring for 20min, obtains final product.
Comparative example 1
The biofungicide that the present invention prevents and treats apple tree canker is substantially the same manner as Example 4, and somewhat different is：The preventing and treating
The biofungicide of apple tree canker, the raw material including following parts by weight is made：
10 parts of Herba Xanthii, 11 parts of the Rhizoma Anemarrhenae, 15 parts of Cortex Mori, 7.5 parts of Fructus Cnidii, 6.5 parts of Fructus Toosendan, 15 parts of dispersant, wetting agent 6
Part, 3 parts of disintegrating agent and 50 parts of filler.
Comparative example 2
The biofungicide that the present invention prevents and treats apple tree canker is substantially the same manner as Example 4, and somewhat different is：
The biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
8 parts of the Radix Pulsatillae, 10 parts of Cortex Magnoliae Officinalis, 7.5 parts of Fructus Cnidii, 6.5 parts of Fructus Toosendan, 15 parts of dispersant, 6 parts of wetting agent, 3 parts of disintegrating agent
With 50 parts of filler.
Comparative example 3
The biofungicide that the present invention prevents and treats apple tree canker is substantially the same manner as Example 4, and somewhat different is：
The biofungicide for preventing and treating apple tree canker, the raw material including following parts by weight is made：
8 parts of the Radix Pulsatillae, 10 parts of Cortex Magnoliae Officinalis, 10 parts of Herba Xanthii, 11 parts of the Rhizoma Anemarrhenae, 15 parts of Cortex Mori, 15 parts of dispersant, 6 parts of wetting agent, disintegrate
3 parts of agent and 50 parts of filler.
Application test
Experimental field：In July, 2015 tests garden, the Fuji apple kind of life in 5 years in a certain Fructus Mali pumilae in Qingdao City.
Reagent agent：Test group is using the apple tree canker of preventing and treating of embodiment of the present invention 1-8 and comparative example 1-3
Biofungicide, formulation rate 50g/ mu, clear water is diluted to medicinal liquid of the concentration for 1mg/mL, safe matched group（CK）Using sterilizing
Water.
Process of the test：Select the card punch punching of 4 mm of smooth branch, the inoculated and cultured canker of apple fruit bacteria cake of 3 days
（d=4 mm）, absorbent cotton dips in water moisturizing, so that bacteria cake is fixed on branch with preservative film winding absorbent cotton, releases moisturizing pass after 3 days
System, selecting the little apple branch of Lesion size difference carries out coating test, often processes 4 scabs of experiment, is repeated 3 times, after 5 days
The extension of investigation scab, calculates extension suppression ratio.
Lesion area=1/4 × π × major diameter × minor axis；
Lesion area extension suppression ratio=（Blank lesion area-process lesion area）/ blank area × 100%.
Prevention effect of 1 embodiment of the present invention 1-8 biofungicide of table to apple tree canker
As shown in Table 1, embodiment of the present invention 1-8 biofungicide is prevented and treated to the suppression ratio of apple tree canker up to more than 90%
Effect is significant, lacks Radix Pulsatillae magnolia obovata in 1 raw material of comparative example, comparative example 2 lacks plate Herba Xanthii, the Rhizoma Anemarrhenae and Cortex Mori
Skin, lacks Fructus Cnidii and Fructus Toosendan in 3 raw material of comparative example, and prevention effect is then decreased obviously, and each raw material association of the present invention is described
Same-action effect is significant.
Claims (10)
1. a kind of biofungicide for preventing and treating apple tree canker, it is characterised in that the raw material including following parts by weight is made：
Radix Pulsatillae 5-10 part, Cortex Magnoliae Officinalis 8-12 part, Herba Xanthii 7-15 part, Rhizoma Anemarrhenae 8-15 part, Cortex Mori 10-20 part, Fructus Cnidii 5-10 part,
Fructus Toosendan 4-8 part, dispersant 10-20 part, wetting agent 3-10 part, disintegrating agent 0-5 part and filler 40-60 part.
2. the biofungicide for preventing and treating apple tree canker according to claim 1, it is characterised in that the dispersant is
In sodium lignin sulfonate, calcium lignosulfonate, oleic acid polyoxyethylene, sodium lauryl sulphate and dodecylbenzene sodium sulfonate extremely
Few one kind.
3. the biofungicide for preventing and treating apple tree canker according to claim 1, it is characterised in that the wetting agent is
Di-2-ethylhexylphosphine oxide how at least one of sodium sulfonate, naphthalene sulfonic acid-formaldehyde condensation product and methyl oleate.
4. the biofungicide for preventing and treating apple tree canker according to claim 1, it is characterised in that the disintegrating agent is
At least one of ammonium sulfate, carbamide and low-substituted hydroxypropyl cellulose.
5. the biofungicide for preventing and treating apple tree canker according to claim 1, it is characterised in that described filler is height
At least one in ridge soil, bentonite, kieselguhr and attapulgite clay.
6. the preparation method of the biofungicide for preventing and treating apple tree canker described in a kind of any one of claim 1-5, which is special
Levy and be, comprise the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）The alcohol solution dipping 10-20h of 5-10 times of weight is added in compound, then under the conditions of 50-60 DEG C
Supersound extraction 2-3 time, each 20-40min；
（3）By step（2）Middle gained extracting solution is evaporated to the 10-20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 20-30min is stirred, through comminution by gas stream,
20-30min is again stirring for, is obtained final product.
7. the preparation method of the biofungicide for preventing and treating apple tree canker according to claim 6, it is characterised in that step
Suddenly（1）The plant material is crushed to particle diameter for 40-80 mesh.
8. the preparation method of the biofungicide for preventing and treating apple tree canker according to claim 6, it is characterised in that step
Suddenly（2）Volumes of aqueous ethanol concentration >=60%.
9. the preparation method of the biofungicide for preventing and treating apple tree canker according to claim 6, it is characterised in that step
Suddenly（2）Supersonic frequency 20-30kHz.
10. the preparation method of the biofungicide for preventing and treating apple tree canker according to claim 6, it is characterised in that
Step（4）The comminution by gas stream to powder diameter is 400-600 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109275660A (en) * | 2018-09-03 | 2019-01-29 | 新昌县云河虫害防治技术开发有限公司 | The preparation method of honey white-out agent |
| CN110278970A (en) * | 2019-07-31 | 2019-09-27 | 黑龙江省农业科学院植物保护研究所 | A kind of Chinese herbal medicine type seed coat agent and preparation method thereof |
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| CN103875675A (en) * | 2014-04-11 | 2014-06-25 | 山东省烟台市农业科学研究院 | Compound composition of cnidium lactone and imazalil and application thereof |
| CN105410061A (en) * | 2014-09-02 | 2016-03-23 | 董继春 | Compound bactericide for preventing and controlling apple tree rot diseases |
| CN107691480A (en) * | 2017-08-30 | 2018-02-16 | 北京联合大学 | A kind of magnolol synergy fungicide for being used to prevent and treat ring spot |
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| CN1040126A (en) * | 1988-08-06 | 1990-03-07 | 徐立 | A kind of bactericide and using method that prevents apple canker |
| CN1695456A (en) * | 2004-05-13 | 2005-11-16 | 河北农业大学 | Application of anemarrhena extractive as bactericide and contained bactericide |
| CN103875675A (en) * | 2014-04-11 | 2014-06-25 | 山东省烟台市农业科学研究院 | Compound composition of cnidium lactone and imazalil and application thereof |
| CN105410061A (en) * | 2014-09-02 | 2016-03-23 | 董继春 | Compound bactericide for preventing and controlling apple tree rot diseases |
| CN107691480A (en) * | 2017-08-30 | 2018-02-16 | 北京联合大学 | A kind of magnolol synergy fungicide for being used to prevent and treat ring spot |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109275660A (en) * | 2018-09-03 | 2019-01-29 | 新昌县云河虫害防治技术开发有限公司 | The preparation method of honey white-out agent |
| CN110278970A (en) * | 2019-07-31 | 2019-09-27 | 黑龙江省农业科学院植物保护研究所 | A kind of Chinese herbal medicine type seed coat agent and preparation method thereof |
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