CN106490064A - A kind of biofungicide of preventing and treating graw mold of tomato and preparation method thereof - Google Patents
A kind of biofungicide of preventing and treating graw mold of tomato and preparation method thereof Download PDFInfo
- Publication number
- CN106490064A CN106490064A CN201610928282.0A CN201610928282A CN106490064A CN 106490064 A CN106490064 A CN 106490064A CN 201610928282 A CN201610928282 A CN 201610928282A CN 106490064 A CN106490064 A CN 106490064A
- Authority
- CN
- China
- Prior art keywords
- parts
- tomato
- biofungicide
- preventing
- graw mold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to technical field of pesticide, and in particular to a kind of biofungicide of preventing and treating graw mold of tomato and preparation method thereof, the biofungicide of the preventing and treating graw mold of tomato includes that the raw material of following parts by weight is made：120 200 parts of 5 10 parts of Fructus Xanthii, 8 12 parts of Folium Caryophylli, 6 10 parts of Folium cinnamomi camphorae, 6 13 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 7 13 parts of Cortex Cinnamomi, 10 15 parts of lemon grass (Cymbopogon citratus), 5 10 parts of Caulis Miscanthis floriduli, 5 15 parts of emulsifying agent, 0 10 parts of synergist and organic solvent.The biofungicide property of medicine of the present invention is stable, is kept for the phase up to 2 years, and noresidue is degradable, and to person poultry harmless, fungistatic effect significantly, to the suppression ratio to graw mold of tomato mycelia up to more than 90%, can be applicable.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of biofungicide of preventing and treating graw mold of tomato and its preparation
Method.
Background technology
Graw mold of tomato is a kind of worldwide important disease, and the disease mainly causes fruit rot, to tomato in greenhouse production
Constitute and greatly threaten.How to be prevented and treated using benzo miaow class and diformazan phthalimide series bactericidal agent in preventing and treating, but continuous use, make
Pathogenic bacteria generates Drug resistance, and preventive effect reduces year by year, also results in pollution to environment.
Chinese patent CN102805115B discloses a kind of plant source natural bactericidal agent for graw mold of tomato, described
Contain Bulbus Allii quintessence oil, plants essential oil mixture, organic solvent, surfactant, water in the raw material of antibacterial.Bulbus Allii in the present invention
Quintessence oil as major antibacterial agent, including two or more other plant quintessence oils as synergist, the killing tomato gray mould that can be improved
The efficiency of disease.But, the patent effective ingredient is single, and bactericidal effect is poor.
Chinese patent CN104782676B discloses a kind of plant protection product, is made up of the crude drug of following weight portion：Raw
Radix Rehmanniae 20-40 parts, Herba Lycopi's 2-6 parts, Cortex Moutan 6-17 parts, Radix Sophorae Flavescentiss 2-13 parts, Herba Emiliae 6-17 parts, Herba Centipedae 2-8 parts, Herba Xanthii
Sub- 2-8 parts, Herba Plantaginiss 1-2 parts, Concretio silicea Bambusae seu schizostachyi 2-6 parts, the male bat 1-2 parts of life.But, the patent protection agent effective ingredient is to tomato gray mould
Sick specific aim is poor, and bactericidal effect is not notable.
Content of the invention
For overcoming drawbacks described above, it is an object of the invention to provide a kind of preventing and treating graw mold of tomato biofungicide and its
Preparation method.
For achieving the above object, the present invention is adopted the following technical scheme that：
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
Fructus Xanthii 5-10 parts, Folium Caryophylli 8-12 part, Folium cinnamomi camphorae 6-10 parts, Folium eucalypti globueli (Eucalyptus globulus Labill.) 6-13 parts, Cortex Cinnamomi 7-13 parts, lemon grass (Cymbopogon citratus) 10-15
Part, Caulis Miscanthis floriduli 5-10 parts, emulsifying agent 5-15 parts, synergist 0-10 parts and organic solvent 120-200 parts.
Preferably, the emulsifying agent is dodecylbenzene sodium sulfonate, NPE, phenethyl phenol Polyethylene oxide
At least one of ether, phenethyl phenylpropyl phenol polyethenoxy ether and tween 80.
Preferably, the synergist is at least one in organosilicon, Butacide, Octacide 264, alkyl carbonate and laurocapram
Kind.
Preferably, the organic solvent is ethanol, glycerol, acetone, Ketohexamethylene, ethyl oleate, ethyl acetate and N, N-
At least one of dimethylformamide.
A kind of preparation method of the biofungicide of preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）The alcohol solution dipping 1-2h, ultrasound assisted extraction 20- of 8-12 times of weight is added in compound
40min, filters, and collects extracting solution；
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 10-20% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Preferably, step（2）Volumes of aqueous ethanol concentration >=75%.
Preferably, step（2）Supersonic frequency 15-25kHz.
The positive beneficial effect of the present invention：
1. Fructus Xanthii acrid in the mouth of the present invention, hardship, warm in nature, antiinflammatory；Folium Caryophylli is bitter, trembles with fear, heat-clearing and toxic substances removing, anti-inflammtory anti-dysentery；Folium cinnamomi camphorae is pungent,
Temperature, wind-dispelling, dehumidifying, removing toxic substances, parasite killing；Folium eucalypti globueli (Eucalyptus globulus Labill.) bitter in the mouth cold in nature, pungent, the effect of with dispelling wind to relieve the exterior syndrome, heat-clearing and toxic substances removing, killing parasites for relieving itching；
Cortex Cinnamomi acrid-sweet flavor, property heat, antibacterial；Lemon grass (Cymbopogon citratus) antibacterial, sterilization, parasite killing；Caulis Miscanthis floriduli antisepsis and sterilization.Above-mentioned each crude drug is shared, medicine
Warm in nature and, significantly, and cream obtained in various adjuvants, the property of medicine are stable, are kept for the phase up to 2 years, and noresidue is easily dropped for insecticidal effect
Solution, to person poultry harmless, fungistatic effect significantly, to the suppression ratio to graw mold of tomato mycelia up to more than 90%, can be applicable.
2. emulsifying agent of the present invention is that the emulsifying agent is dodecylbenzene sodium sulfonate, NPE, benzene
At least one of ethylo benzene propyl group phenol polyethenoxy ether and tween 80, make various raw materials more preferably miscible in organic solvent.Described
Synergist is at least one of organosilicon, Butacide, Octacide 264, alkyl carbonate and laurocapram, with low surface tension,
Good plate-out ability, permeability and emulsifying dispersivity, effectively suppress or reduction pathogenic bacteria is to the anti-poisoning effect of the removing toxic substances of pesticide activity.
The organic solvent be ethanol, glycerol, acetone, Ketohexamethylene, ethyl oleate, ethyl acetate and DMF in extremely
Few one kind is abundant to various material dissolutions.
3. completely, efficacy stability is with low cost for each plant material extracts active ingredients of the invention.
Specific embodiment
With reference to some specific embodiments, the present invention is further described.
Embodiment 1
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
5 parts of Fructus Xanthii, 8 parts of Folium Caryophylli, 6 parts of Folium cinnamomi camphorae, 6 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 7 parts of Cortex Cinnamomi, 10 parts of lemon grass (Cymbopogon citratus), 5 parts of Caulis Miscanthis floriduli, emulsifying agent
5 parts and 120 parts of organic solvent.
The emulsifying agent is dodecylbenzene sodium sulfonate.
The organic solvent is ethanol.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）75% alcohol solution dipping 1h of 8 times of weight, ultrasound assisted extraction 40min, mistake is added in compound
Filter, collects extracting solution；Supersonic frequency 15kHz.
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 10% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Embodiment 2
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
5 parts of Fructus Xanthii, 8 parts of Folium Caryophylli, 6 parts of Folium cinnamomi camphorae, 6 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 7 parts of Cortex Cinnamomi, 10 parts of lemon grass (Cymbopogon citratus), 5 parts of Caulis Miscanthis floriduli, emulsifying agent
5 parts, 120 parts of 1 part of synergist and organic solvent.
The emulsifying agent is NPE.
The synergist is organosilicon.
The organic solvent is glycerol.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）80% alcohol solution dipping 1.5h, the ultrasound assisted extraction 30min of 10 times of weight is added in compound,
Filter, collect extracting solution；Supersonic frequency 20kHz.
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 15% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Embodiment 3
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
6 parts of Fructus Xanthii, 9 parts of Folium Caryophylli, 7 parts of Folium cinnamomi camphorae, 7 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 8 parts of Cortex Cinnamomi, 11 parts of lemon grass (Cymbopogon citratus), 6 parts of Caulis Miscanthis floriduli, emulsifying agent
7 parts, 130 parts of 2 parts of synergist and organic solvent.
The emulsifying agent is phenethyl phenol polyethenoxy ether.
The synergist is Octacide 264.
The organic solvent is acetone.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）85% alcohol solution dipping 2h of 12 times of weight, ultrasound assisted extraction 20min, mistake is added in compound
Filter, collects extracting solution；Supersonic frequency 25kHz.
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 20% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Embodiment 4
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
7 parts of Fructus Xanthii, 10 parts of Folium Caryophylli, 8 parts of Folium cinnamomi camphorae, 9 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 10 parts of Cortex Cinnamomi, 12 parts of lemon grass (Cymbopogon citratus), 7 parts of Caulis Miscanthis floriduli, emulsifying
140 parts of 8 parts of agent, 5 parts of synergist and organic solvent.
The emulsifying agent is NPE.
The synergist is laurocapram.
The organic solvent is Ketohexamethylene.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）90% alcohol solution dipping 1.5h, the ultrasound assisted extraction 40min of 10 times of weight is added in compound,
Filter, collect extracting solution；Supersonic frequency 20kHz.
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 15% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Embodiment 5
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
7 parts of Fructus Xanthii, 10 parts of Folium Caryophylli, 8 parts of Folium cinnamomi camphorae, 9 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 10 parts of Cortex Cinnamomi, 12 parts of lemon grass (Cymbopogon citratus), 7 parts of Caulis Miscanthis floriduli, Dens Equi
140 parts of 10 parts of Herba Amaranthi tricoloriss, 8 parts of emulsifying agent, 5 parts of synergist and organic solvent.
Add Herba Portulacae in the present embodiment, Herba Portulacae acid is cold, heat-clearing and toxic substances removing, diuretic damp eliminating, scattered blood detumescence, Pesticidal and sterilizing,
Share with various raw materials, enhance the fungistatic effect of cream.
The emulsifying agent is NPE.
The synergist is laurocapram.
The organic solvent is Ketohexamethylene.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato is shown in embodiment 4.
Embodiment 6
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
8 parts of Fructus Xanthii, 11 parts of Folium Caryophylli, 8 parts of Folium cinnamomi camphorae, 10 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 11 parts of Cortex Cinnamomi, 13 parts of lemon grass (Cymbopogon citratus), 7 parts of Caulis Miscanthis floriduli, breast
150 parts of 10 parts of agent, 7 parts of synergist and organic solvent.
The emulsifying agent is phenethyl phenylpropyl phenol polyethenoxy ether.
The synergist is alkyl carbonate.
The organic solvent is ethyl oleate.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）95% alcohol solution dipping 2h of 11 times of weight, ultrasound assisted extraction 30min, mistake is added in compound
Filter, collects extracting solution；Supersonic frequency 25kHz.
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 20% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Embodiment 7
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
9 parts of Fructus Xanthii, 11.5 parts of Folium Caryophylli, 9 parts of Folium cinnamomi camphorae, 12 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 12 parts of Cortex Cinnamomi, 14 parts of lemon grass (Cymbopogon citratus), 9 parts of Caulis Miscanthis floriduli,
160 parts of 13 parts of emulsifying agent, 8 parts of synergist and organic solvent.
The emulsifying agent is dodecylbenzene sodium sulfonate and NPE, and both weight ratios are 1:1.
The synergist is Butacide.
The organic solvent is DMF.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）Straight alcohol immersion 1h, the ultrasound assisted extraction 20min of 10 times of weight is added in compound, is filtered, is received
Collection extracting solution；Supersonic frequency 15kHz.
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 10% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Embodiment 8
A kind of biofungicide of preventing and treating graw mold of tomato, the raw material including following parts by weight are made：
10 parts of Fructus Xanthii, 12 parts of Folium Caryophylli, 10 parts of Folium cinnamomi camphorae, 13 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 13 parts of Cortex Cinnamomi, 15 parts of lemon grass (Cymbopogon citratus), 10 parts of Caulis Miscanthis floriduli,
200 parts of 15 parts of emulsifying agent, 10 parts of synergist and organic solvent.
The emulsifying agent is tween 80.
The synergist is Butacide and laurocapram, and both weight ratios are 1：1.
The organic solvent is ethyl acetate.
The preparation method of the biofungicide of above-mentioned preventing and treating graw mold of tomato, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）75% alcohol solution dipping 2h of 12 times of weight, ultrasound assisted extraction 40min, mistake is added in compound
Filter, collects extracting solution；Supersonic frequency 20kHz.
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 20% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
Comparative example 1
The biofungicide of the present embodiment preventing and treating graw mold of tomato is substantially the same manner as Example 4, and difference is：The preventing and treating
The biofungicide of graw mold of tomato, the raw material including following parts by weight are made：
10 parts of Folium Caryophylli, 8 parts of Folium cinnamomi camphorae, 9 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 12 parts of lemon grass (Cymbopogon citratus), 7 parts of Caulis Miscanthis floriduli, 8 parts of emulsifying agent and have 5 parts of synergist
140 parts of machine solvent.
Comparative example 2
The biofungicide of the present embodiment preventing and treating graw mold of tomato is substantially the same manner as Example 4, and difference is：The preventing and treating
The biofungicide of graw mold of tomato, the raw material including following parts by weight are made：
7 parts of Fructus Xanthii, 10 parts of Cortex Cinnamomi, 12 parts of lemon grass (Cymbopogon citratus), 7 parts of Caulis Miscanthis floriduli, 8 parts of emulsifying agent, 5 parts of synergist and organic solvent 140
Part.
Comparative example 3
The biofungicide of the present embodiment preventing and treating graw mold of tomato is substantially the same manner as Example 4, and difference is：The preventing and treating
The biofungicide of graw mold of tomato, the raw material including following parts by weight are made：
7 parts of Fructus Xanthii, 10 parts of Folium Caryophylli, 8 parts of Folium cinnamomi camphorae, 9 parts of Folium eucalypti globueli (Eucalyptus globulus Labill.), 10 parts of Cortex Cinnamomi, 8 parts of emulsifying agent, 5 parts of synergist and organic
140 parts of solvent.
Application test
Test method：The inhibitory action that each embodiment medicament is grown to graw mold of tomato mycelia will be determined using growth rate method,
Experimental group prevents and treats the biofungicide of graw mold of tomato using embodiment of the present invention 1-8, matched group 1-3, and clear water dilutes, and safety is right
According to group（CK）Using aquesterilisa, reagent agent is added in 40-50 DEG C of PDA culture medium, is mixed, content is paved in culture dish
For the malicious flat board of 10% band, 3 repetitions are often processed, the pathogenic fungi pure culture biscuits involvng inoculation of a diameter of 6mm is hit exactly in flat board, 25 DEG C of constant temperature
Cultivate in incubator, 3d measures each colony diameter, experimental result is shown in Table 1.
Colony diameter=measurement diameter -6；
Mycelial growth inhibition rate=（Control colony diameter-process colony diameter）/ control colony diameter × 100%.
Prevention effect of 1 embodiment of the present invention 1-8 biofungicide of table to graw mold of tomato
As shown in Table 1, embodiment of the present invention 1-8 biofungicide is prevented to the suppression ratio of graw mold of tomato mycelia up to more than 90%
Effect is significant is controlled, is lacked Fructus Xanthii and Cortex Cinnamomi in 1 raw material of comparative example, in 2 raw material of comparative example, is lacked Folium Caryophylli, Lignum cinnamomi camphorae
Leaf and Folium eucalypti globueli (Eucalyptus globulus Labill.), lack lemon grass (Cymbopogon citratus) and Caulis Miscanthis floriduli in 3 raw material of comparative example, and prevention effect is then decreased obviously, and illustrate of the invention each
Raw material synergistic action effect is notable.
Claims (7)
1. a kind of preventing and treating graw mold of tomato biofungicide, it is characterised in that the raw material including following parts by weight is made：
Fructus Xanthii 5-10 parts, Folium Caryophylli 8-12 part, Folium cinnamomi camphorae 6-10 parts, Folium eucalypti globueli (Eucalyptus globulus Labill.) 6-13 parts, Cortex Cinnamomi 7-13 parts, lemon grass (Cymbopogon citratus) 10-15
Part, Caulis Miscanthis floriduli 5-10 parts, emulsifying agent 5-15 parts, synergist 0-10 parts and organic solvent 120-200 parts.
2. according to claim 1 preventing and treating graw mold of tomato biofungicide, it is characterised in that the emulsifying agent be ten
Dialkyl benzene sulfonic acids sodium, NPE, phenethyl phenol polyethenoxy ether, phenethyl phenylpropyl phenol polyethenoxy ether and
At least one of tween 80.
3. the biofungicide of preventing and treating graw mold of tomato according to claim 1, it is characterised in that the synergist is for having
At least one of machine silicon, Butacide, Octacide 264, alkyl carbonate and laurocapram.
4. the biofungicide of preventing and treating graw mold of tomato according to claim 1, it is characterised in that the organic solvent is
At least one of ethanol, glycerol, acetone, Ketohexamethylene, ethyl oleate, ethyl acetate and DMF.
5. described in a kind of any one of claim 1-4 preventing and treating graw mold of tomato biofungicide preparation method, its feature
It is, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）The alcohol solution dipping 1-2h, ultrasound assisted extraction 20- of 8-12 times of weight is added in compound
40min, filters, and collects extracting solution；
（3）By step（2）Middle gained extracting solution cryogenic vacuum is concentrated into the 10-20% of original solution volume, obtains plant extract；
（4）Organic solvent, emulsifying agent are added in plant extract, is stirred, is obtained final product.
6. according to claim 5 preventing and treating graw mold of tomato biofungicide preparation method, it is characterised in that step
（2）Volumes of aqueous ethanol concentration >=75%.
7. according to claim 5 preventing and treating graw mold of tomato biofungicide preparation method, it is characterised in that step
（2）Supersonic frequency 15-25kHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610928282.0A CN106490064A (en) | 2016-10-31 | 2016-10-31 | A kind of biofungicide of preventing and treating graw mold of tomato and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610928282.0A CN106490064A (en) | 2016-10-31 | 2016-10-31 | A kind of biofungicide of preventing and treating graw mold of tomato and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106490064A true CN106490064A (en) | 2017-03-15 |
Family
ID=58318639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610928282.0A Pending CN106490064A (en) | 2016-10-31 | 2016-10-31 | A kind of biofungicide of preventing and treating graw mold of tomato and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106490064A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375112A (en) * | 2017-08-31 | 2017-11-24 | 秦得翔 | A kind of washing lotion for preventing and treating cat lice and its application |
| CN107788023A (en) * | 2017-12-08 | 2018-03-13 | 柳州雅瑞科技有限公司 | A kind of botanical fungicide for preventing and treating edible lily gray mold and preparation method thereof |
| CN112322091A (en) * | 2020-11-23 | 2021-02-05 | 成都市富百乐装饰材料实业有限公司 | Air purification, sterilization and mildew-proof coating and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1212126A (en) * | 1998-08-12 | 1999-03-31 | 赵常安 | Crop bactericide containing clove oil and eugenol |
| KR20070051427A (en) * | 2005-11-15 | 2007-05-18 | (주)대덕바이오 | Antifungal compound containing cinnamic aldehyde extracted from cinnamomum cassia |
| KR20070065935A (en) * | 2005-11-11 | 2007-06-27 | (주)대덕바이오 | Anti-fungal compound containing eugenol extracted from eugenia caryophyllata thumb |
| CN102805115A (en) * | 2012-08-30 | 2012-12-05 | 白静 | Plant-source natural fungicide for preventing and controlling botrytis cinerea and preparation method thereof |
| CN103609600A (en) * | 2013-11-11 | 2014-03-05 | 李文斌 | High efficiency germicide of botrytis cinerea and preparation method thereof |
| CN104336094A (en) * | 2013-08-05 | 2015-02-11 | 天津市滨河蔬菜种植专业合作社 | Tomato grey mold controlling agent and preparation method thereof |
| CN104782676A (en) * | 2015-03-19 | 2015-07-22 | 陕西乾龙高科生态农业有限公司 | Plant protective agent and preparation method thereof |
-
2016
- 2016-10-31 CN CN201610928282.0A patent/CN106490064A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1212126A (en) * | 1998-08-12 | 1999-03-31 | 赵常安 | Crop bactericide containing clove oil and eugenol |
| KR20070065935A (en) * | 2005-11-11 | 2007-06-27 | (주)대덕바이오 | Anti-fungal compound containing eugenol extracted from eugenia caryophyllata thumb |
| KR20070051427A (en) * | 2005-11-15 | 2007-05-18 | (주)대덕바이오 | Antifungal compound containing cinnamic aldehyde extracted from cinnamomum cassia |
| CN102805115A (en) * | 2012-08-30 | 2012-12-05 | 白静 | Plant-source natural fungicide for preventing and controlling botrytis cinerea and preparation method thereof |
| CN104336094A (en) * | 2013-08-05 | 2015-02-11 | 天津市滨河蔬菜种植专业合作社 | Tomato grey mold controlling agent and preparation method thereof |
| CN103609600A (en) * | 2013-11-11 | 2014-03-05 | 李文斌 | High efficiency germicide of botrytis cinerea and preparation method thereof |
| CN104782676A (en) * | 2015-03-19 | 2015-07-22 | 陕西乾龙高科生态农业有限公司 | Plant protective agent and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 林珲 等: "《流域管理科学化的探索与实践》", 31 December 2000, 江西科学技术出版社 * |
| 王宏年 等: "7种植物精油对番茄灰霉病的抑制效果", 《植物保护》 * |
| 辽宁省林业学校: "《森林病理学（第2版）》", 31 December 1991, 中国林业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375112A (en) * | 2017-08-31 | 2017-11-24 | 秦得翔 | A kind of washing lotion for preventing and treating cat lice and its application |
| CN107788023A (en) * | 2017-12-08 | 2018-03-13 | 柳州雅瑞科技有限公司 | A kind of botanical fungicide for preventing and treating edible lily gray mold and preparation method thereof |
| CN112322091A (en) * | 2020-11-23 | 2021-02-05 | 成都市富百乐装饰材料实业有限公司 | Air purification, sterilization and mildew-proof coating and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105994450A (en) | Chinese herbal medicine insecticide of tea and preparation method thereof | |
| CN103548895A (en) | Botanical vegetable insecticide | |
| CN105594765A (en) | Environment-friendly pesticide | |
| CN106135318A (en) | A kind of Chinese medicine composition and its preparation method and application | |
| CN102812972B (en) | Plant extract composition for preventing and treating apple tree canker, and preparation method thereof | |
| CN106818905A (en) | A kind of cultivation Chinese herbal disinfectant and preparation method thereof | |
| CN107969443A (en) | A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament | |
| CN106490064A (en) | A kind of biofungicide of preventing and treating graw mold of tomato and preparation method thereof | |
| CN105076253A (en) | Composite growth regulator special for cucumbers | |
| CN106490020A (en) | A kind of Microbial wettable powder of preventing and treating Fructus Kaki mealybug and preparation method thereof | |
| CN105685140A (en) | An insect-resist agent and a preparing method thereof | |
| CN103598266B (en) | Non-polluted pesticide with pure traditional Chinese medicinal components, as well as preparation method and application thereof | |
| CN104886163A (en) | Botanical pesticide compound phytolacca acinosa and magnolia officinalis microemulsion and preparation method thereof | |
| CN104255826A (en) | Application of multifunctional botanical fungicide in preventing and treating iris tectorum crown rot | |
| CN102948434A (en) | Aquaculture sterilization parasiticide | |
| CN106259482A (en) | A kind of Chinese medicinal plant bactericidal composition and preparation method | |
| CN106561717A (en) | Pesticide for preventing and controlling ginger blast, and preparation method thereof | |
| CN1401235A (en) | Aqueous berberine-matrine fungicidal and insecticidal solution | |
| CN103355326A (en) | High-efficiency pesticide composition | |
| CN104522059A (en) | Phytolacca americana extract and application of phytolacca americana extract in preventing and treating dalbergia odorifera anthracnose | |
| KR20110022029A (en) | Natural germicide manufacturing method | |
| CN101849549B (en) | Pesticide preparation for preventing and treating rice hoppers and other rice pests | |
| CN106417267A (en) | Fungicide controlling take-all disease of wheat | |
| CN106417412A (en) | Biological bacterial killing agent for control of apple tree growth ring disease and preparation method thereof | |
| CN106490014A (en) | A kind of plant compound pesticide containing ricidine. and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170315 |