CN107969443A - A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament - Google Patents
A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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Abstract
The invention belongs to control of agricultural pest field, and in particular to a kind of preparation method for preventing Citrus Huanglongbing pathogen medicament.Habit of the invention first with wood louse, citrus tender shoots etc. is extracted, orange peel and peppermint are added in extraction process, wood louse is attracted, lure in liquid containing chinaberry after wood louse is carried out attraction come, wood louse kill removing by it, wood louse is sticked using mixed liquor stickiness, and lure in liquid containing abundant nutriment absorbing for citrus, then the materials such as eggplant leaf are extracted, compounded by the material in extract and essential oil, it is diffused extract, attract the natural enemy of wood louse, wood louse kill and removes and prevents, and the essential oil added has the effect of driveing to wood louse, the present invention avoids using chemicals to kill except diaphorina citri efficacy time is short at present, easily lead to diaphorina citri and the resistance to the action of a drug is generated to a large amount of pesticides, insecticidal effect declines the problem of obvious, safe non-hazardous.
Description
Technical field
The invention belongs to control of agricultural pest field, and in particular to a kind of preparation side for preventing Citrus Huanglongbing pathogen medicament
Method.
Background technology
China is Orange Producing big country in the world, and Citrus Industry is China's Major agro-industry, is accounted in agricultural economy
There is extremely important status, played in increasing peasant income, increase social employment and building Socialist New is promoted extremely heavy
The effect wanted.Citrus pest and disease damage is the major obstacles of Aspects In The Development of Citrus Industry, wherein the most serious is be referred to as citrus " cancer "
Citrus Huanglongbing pathogen.With the fast development of Orange Producing, the generation of Citrus Huanglongbing pathogen and cause harm increasingly severe.The disease is a kind of
To cause harm great contagious disease, germ is propagated by diaphorina citri, and popular duration is long, morbidity scope is wide, incidence is high for it,
Cause China's most area citrus short life, low output, production cost high, annual economic loss is up to multi-million dollar, sternly
Perplex and restrict the development of Citrus Industry in China or even world wide again.
The main media for propagating yellow twig is diaphorina citri.Diaphorina citri belongs to Homoptera, and Psyllidae, it is to propagate citrus
The main insect vector of yellow twig, is the main reason for causing Citrus Huanglongbing pathogen to propagate, spread.Diaphorina citri feeding band poison is tender
Can pass poison after leaf 2h, single adult passes sick rate and is up to 70% ~ 80%, diaphorina citri have efficiently pass disease, lifelong band poison, with
The features such as wind closely spreads and quickly passes poison.It was verified that just there is Citrus Huanglongbing pathogen in the place for having diaphorina citri, citrus is controlled
Wood louse, cut-out disease spread are the important measures of current control Citrus Huanglongbing pathogen.So prevent and kill off diaphorina citri using chemical agent
It is the most important method of prevention and control Citrus Huanglongbing pathogen.However, present biological pesticide, efficacy time is short, substantially no more than 6d, frequently
Laxative both added the burden of orchard worker, also increase labor cost, also cause diaphorina citri to generate a large amount of pesticides anti-
Pharmacological property, insecticidal effect decline obvious.Therefore, producing a kind of high prevention effect of drug effect, good medicament is that current Citrus Huanglongbing pathogen is prevented
The task of top priority of control.
The content of the invention
The technical problems to be solved by the invention：For being killed at present using chemicals except diaphorina citri, it is yellow to prevent citrus
Imperial disease, although there is certain effect, efficacy time is short, and frequently laxative had both added the burden of orchard worker, causes citrus wooden
Lice generates a large amount of pesticides the resistance to the action of a drug, and insecticidal effect declines the problem of obvious, and the present invention provides one kind prevention citrus Huanglong
The preparation method of sick medicament.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament, the preparation method include the following steps：
（1）Count in parts by weight, take 80~90 parts of citrus tender shoots, 30~40 parts of Chinese parasol tree tender shoots, 20~25 parts of orange peels, 10~15
Part chinaberry and 6~9 portions of peppermints, are put into mixer and are stirred, and dry, mill, and collect thing of milling；
（2）To be milled thing and glucose solution in mass ratio 1：3~5 are uniformly mixed, and are put into fermentation tank, add material of milling
The bacterium powder of amount 6~8%, ferments 1~2 day at 30~38 DEG C, collects fermenting mixture, carries out steam blasting, collects explosion thing；
（3）By explosion thing, edible fly maggot and ethanol solution in mass ratio 5：1：8 are put into ball mill and carry out ball milling, collect ball milling
Thing, is placed in standing at 45~50 DEG C, filtering, collect filtrate, rotary evaporation recycling ethanol, collect residue, by residue with
Mixed liquor in mass ratio 6：1~3 is uniformly mixed, and sterilizing, must lure liquid, spare；
（4）Count in parts by weight, take 60~63 parts of eggplant leaves, 50~55 parts of potato leafs, 26~32 parts of Commeline communis, 19~23 parts
Nettle is uniformly mixed, and dries, and crushes, and crushed material is collected, by crushed material and 1.2mol/L ethanol solutions in mass ratio 1：3 mixing,
Supercritical carbon dioxide extracting is carried out, collects extract, filtering, collects filtrate, rotary evaporation recycling ethanol, will evaporate residue
With essential oil in mass ratio 4：1 is uniformly mixed, and obtains protection liquid；
（5）By step（3）Spare lures liquid and protection liquid in mass ratio 4：3~5, bottle respectively, up to citrus Huanglong is prevented
Sick medicament.
The step（2）Middle bacterium powder is any one in yeast bacterium powder, aspergillus oryzae bacterium powder.
The step（2）Middle steam blasting condition is 120~125 DEG C, 1.6~2.0MPa of pressure of temperature, maintenance pressure 1~
3min。
The step（3）Middle mixed liquor is in mass ratio 1：2：5~8, by zeins, sodium alginate and
0.2mol/L sodium phosphates are uniformly mixed, up to mixed liquor.
The step（4）Middle essential oil is any one in marigold essential oil, galic essential oil.
The prevention Citrus Huanglongbing pathogen medicament when in use, will first lure liquid and water in mass ratio 1：13 mixing, are sprayed on
On mandarin tree, after spraying 2~4h, then will protection liquid and water in mass ratio 1：7 mixing, are sprayed on mandarin tree.
Compared with other methods, advantageous effects are the present invention：
Habit of the invention first with wood louse, extracts citrus tender shoots, Chinese parasol tree tender shoots, obtains the material for attracting wood louse,
Orange peel and peppermint are added in extraction process, adds and lures diffusion component in liquid, citrus tender shoots is carried using diffusion component
It is diffused with the extract of Chinese parasol tree tender shoots, wood louse is attracted, and lured and contain chinaberry in liquid, by chinaberry
Biology extraction, obtain wherein insect killing substance, after wood louse is carried out attraction come, wood louse kill removing by it, in order to
Retention further is carried out to wood louse, mixed liquor is added in liquid is lured, wood louse is sticked using mixed liquor stickiness, and
Lure and contain abundant nutriment absorbing for citrus in liquid, increase the resistance of body, then to eggplant leaf, horse
The materials such as bell potato leaf are extracted, and obtain material therein, are compounded by the material in extract with essential oil, make extract
Be diffused, attract the natural enemy of wood louse, wood louse kill and removes and prevents, and the essential oil added to wood louse have drive work
With the present invention to wood louse by attracting to kill to remove, and natural enemy prevention, natural processing method, is avoided and killed at present using chemicals
Except diaphorina citri efficacy time is short, and frequently laxative had both added the burden of orchard worker, caused diaphorina citri to produce a large amount of pesticides
The resistance to the action of a drug is given birth to, insecticidal effect declines the problem of obvious, safe non-hazardous.
Embodiment
The selection of bacterium powder：Any one in yeast bacterium powder, aspergillus oryzae bacterium powder.
The step（3）Middle mixed liquor is in mass ratio 1：2：5~8, by zeins, sodium alginate and
0.2mol/L sodium phosphates are uniformly mixed, up to mixed liquor.
The step（4）Middle essential oil is any one in marigold essential oil, galic essential oil.
A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament, the preparation method include the following steps：
（1）Count in parts by weight, take 80~90 parts of citrus tender shoots, 30~40 parts of Chinese parasol tree tender shoots, 20~25 parts of orange peels, 10~15
Part chinaberry and 6~9 portions of peppermints, are put into mixer and are stirred, and dry 50min at 60 DEG C, are milled with 600r/min
30min, collects thing of milling；
（2）To be milled thing and glucose solution in mass ratio 1：3~5 are uniformly mixed, and are put into fermentation tank, add material of milling
The bacterium powder of amount 6~8%, ferments 1~2 day at 30~38 DEG C, collects fermenting mixture, carries out steam blasting, steam blasting bar
Part is 120~125 DEG C, 1.6~2.0MPa of pressure of temperature, maintains 1~3min of pressure, collects explosion thing；
（3）By explosion thing, edible fly maggot and ethanol solution in mass ratio 5：1：8 are put into ball mill with 800r/min progress ball millings
30min, collects ball milling thing, is placed in standing 2h at 45~50 DEG C, filters, and collects filtrate, rotary evaporation recycling ethanol, is collected surplus
Excess, by residue and mixed liquor in mass ratio 6：1~3 is uniformly mixed, and sterilizing, must lure liquid, spare；
（4）Count in parts by weight, take 60~63 parts of eggplant leaves, 50~55 parts of potato leafs, 26~32 parts of Commeline communis, 19~23 parts
Nettle is uniformly mixed, and dries, and crushes, and crushed material is collected, by crushed material and 1.2mol/L ethanol solutions in mass ratio 1：3 mixing,
Supercritical carbon dioxide extracting is carried out, collects extract, filtering, collects filtrate, rotary evaporation recycling ethanol, will evaporate residue
With essential oil in mass ratio 4：1 is uniformly mixed, and obtains protection liquid；
（5）By step（3）Spare lures liquid and protection liquid in mass ratio 4：3~5, bottle respectively, up to citrus Huanglong is prevented
Sick medicament.
The prevention Citrus Huanglongbing pathogen medicament of the present invention when in use, will first lure liquid and water in mass ratio 1：13 mixing, spray
Spill on mandarin tree, after spraying 2~4h, then liquid and water in mass ratio 1 will be protected：7 mixing, are sprayed on mandarin tree.
Embodiment 1
The selection of bacterium powder：Aspergillus oryzae bacterium powder.
Mixed liquor is formulated as in mass ratio 1：2：7, zeins, sodium alginate and 0.2mol/L sodium phosphates are mixed
Close uniformly, up to mixed liquor.
The selected as marigold essential oil of essential oil.
A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament, the preparation method include the following steps：
（1）Count in parts by weight, take 90 parts of citrus tender shoots, 40 parts of Chinese parasol tree tender shoots, 25 parts of orange peels, 15 parts of chinaberrys and 9 parts thin
Lotus, is put into mixer and is stirred, and dries 50min at 60 DEG C, is milled 30min with 600r/min, and collection is milled thing；
（2）To be milled thing and glucose solution in mass ratio 1：5 are uniformly mixed, and are put into fermentation tank, add amount of substance of milling
8% bacterium powder, ferments 2 days at 38 DEG C, collects fermenting mixture, carries out steam blasting, steam blasting condition is temperature 125
DEG C, pressure 2.0MPa, maintains pressure 3min, collects explosion thing；
（3）By explosion thing, edible fly maggot and ethanol solution in mass ratio 5：1：8 are put into ball mill with 800r/min progress ball millings
30min, collects ball milling thing, is placed in standing 2h at 50 DEG C, filters, and collects filtrate, rotary evaporation recycling ethanol, is collected remaining
Thing, by residue and mixed liquor in mass ratio 6：1~3 is uniformly mixed, and sterilizing, must lure liquid, spare；
（4）Count in parts by weight, take 63 parts of eggplant leaves, 55 parts of potato leafs, 32 parts of Commeline communis, 23 portions of nettles to be uniformly mixed, dry
It is dry, crush, crushed material is collected, by crushed material and 1.2mol/L ethanol solutions in mass ratio 1：3 mixing, carry out overcritical titanium dioxide
Carbon extracts, and collects extract, filtering, collects filtrate, rotary evaporation recycling ethanol, by evaporation residue and essential oil in mass ratio 4：
1 is uniformly mixed, and obtains protection liquid；
（5）By step（3）Spare lures liquid and protection liquid in mass ratio 4：5, bottle respectively, up to Citrus Huanglongbing pathogen medicine is prevented
Agent.
The prevention Citrus Huanglongbing pathogen medicament of the present invention when in use, will first lure liquid and water in mass ratio 1：13 mixing, spray
Spill on mandarin tree, after spraying 4h, then liquid and water in mass ratio 1 will be protected：7 mixing, are sprayed on mandarin tree.
Embodiment 2
The selection of bacterium powder：Aspergillus oryzae bacterium powder.
Mixed liquor is in mass ratio 1：2：6, zeins, sodium alginate and 0.2mol/L sodium phosphates are mixed equal
It is even, up to mixed liquor.
The selected as galic essential oil of essential oil.
A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament, the preparation method include the following steps：
（1）Count in parts by weight, take 85 parts of citrus tender shoots, 35 parts of Chinese parasol tree tender shoots, 23 parts of orange peels, 13 parts of chinaberrys and 8 parts thin
Lotus, is put into mixer and is stirred, and dries 50min at 60 DEG C, is milled 30min with 600r/min, and collection is milled thing；
（2）To be milled thing and glucose solution in mass ratio 1：4 are uniformly mixed, and are put into fermentation tank, add amount of substance of milling
7% bacterium powder, ferments 2 days at 35 DEG C, collects fermenting mixture, carries out steam blasting, steam blasting condition is temperature 123
DEG C, pressure 1.8MPa, maintains pressure 2min, collects explosion thing；
（3）By explosion thing, edible fly maggot and ethanol solution in mass ratio 5：1：8 are put into ball mill with 800r/min progress ball millings
30min, collects ball milling thing, is placed in standing 2h at 48 DEG C, filters, and collects filtrate, rotary evaporation recycling ethanol, is collected remaining
Thing, by residue and mixed liquor in mass ratio 6：2 are uniformly mixed, and sterilizing, must lure liquid, spare；
（4）Count in parts by weight, take 62 parts of eggplant leaves, 53 parts of potato leafs, 28 parts of Commeline communis, 21 portions of nettles to be uniformly mixed, dry
It is dry, crush, crushed material is collected, by crushed material and 1.2mol/L ethanol solutions in mass ratio 1：3 mixing, carry out overcritical titanium dioxide
Carbon extracts, and collects extract, filtering, collects filtrate, rotary evaporation recycling ethanol, by evaporation residue and essential oil in mass ratio 4：
1 is uniformly mixed, and obtains protection liquid；
（5）By step（3）Spare lures liquid and protection liquid in mass ratio 4：4, bottle respectively, up to Citrus Huanglongbing pathogen medicine is prevented
Agent.
The prevention Citrus Huanglongbing pathogen medicament of the present invention when in use, will first lure liquid and water in mass ratio 1：13 mixing, spray
Spill on mandarin tree, after spraying 3h, then liquid and water in mass ratio 1 will be protected：7 mixing, are sprayed on mandarin tree.
Embodiment 3
The selection of bacterium powder：Yeast bacterium powder.
Mixed liquor is formulated as in mass ratio 1：2：5, zeins, sodium alginate and 0.2mol/L sodium phosphates are mixed
Close uniformly, up to mixed liquor.
The selected as marigold essential oil of essential oil.
A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament, the preparation method include the following steps：
（1）Count in parts by weight, take 80 parts of citrus tender shoots, 30 parts of Chinese parasol tree tender shoots, 20 parts of orange peels, 10 parts of chinaberrys and 6 parts thin
Lotus, is put into mixer and is stirred, and dries 50min at 60 DEG C, is milled 30min with 600r/min, and collection is milled thing；
（2）To be milled thing and glucose solution in mass ratio 1：3 are uniformly mixed, and are put into fermentation tank, add amount of substance of milling
6% bacterium powder, ferments 1 day at 30 DEG C, collects fermenting mixture, carries out steam blasting, steam blasting condition is temperature 120
DEG C, pressure 1.6MPa, maintains pressure 1min, collects explosion thing；
（3）By explosion thing, edible fly maggot and ethanol solution in mass ratio 5：1：8 are put into ball mill with 800r/min progress ball millings
30min, collects ball milling thing, is placed in standing 2h at 45 DEG C, filters, and collects filtrate, rotary evaporation recycling ethanol, is collected remaining
Thing, by residue and mixed liquor in mass ratio 6：1 is uniformly mixed, and sterilizing, must lure liquid, spare；
（4）Count in parts by weight, take 60 parts of eggplant leaves, 50 parts of potato leafs, 26 parts of Commeline communis, 19 portions of nettles to be uniformly mixed, dry
It is dry, crush, crushed material is collected, by crushed material and 1.2mol/L ethanol solutions in mass ratio 1：3 mixing, carry out overcritical titanium dioxide
Carbon extracts, and collects extract, filtering, collects filtrate, rotary evaporation recycling ethanol, by evaporation residue and essential oil in mass ratio 4：
1 is uniformly mixed, and obtains protection liquid；
（5）By step（3）Spare lures liquid and protection liquid in mass ratio 4：3, bottle respectively, up to Citrus Huanglongbing pathogen medicine is prevented
Agent.
The prevention Citrus Huanglongbing pathogen medicament of the present invention when in use, will first lure liquid and water in mass ratio 1：13 mixing, spray
Spill on mandarin tree, after spraying 2h, then liquid and water in mass ratio 1 will be protected：7 mixing, are sprayed on mandarin tree.
Comparative example：The medicament of the prevention Citrus Huanglongbing pathogen of Guangzhou company production.
1 pair of adjacent orchard is selected in Jiangxi Province Poyang County, carries out follow-up experiment observation.There are 1000 satsuma oranges in orchard,
The age of tree 10 years, two fruits weather, ecology, soil, farming management level are consistent.Two orchard yellow twigs are investigated, incidence is below
1%, and disease is set to surgery.The prevention yellow twig medicament of the present invention is respectively adopted and the medicament of reference examples is sprayed on the 1st June
Testing tries, experimental result such as table 1.
Table 1：
From the data of embodiment 1 ~ 3, embodiment 1 is optimum embodiment, the medicament effect of the prevention Citrus Huanglongbing pathogen obtained
Fruit is best.Summary, product properties of the invention are superior to presently commercially available product, and be worth promoting use.
Claims (6)
1. a kind of preparation method for preventing Citrus Huanglongbing pathogen medicament, it is characterised in that the preparation method includes the following steps：
（1）Count in parts by weight, take 80~90 parts of citrus tender shoots, 30~40 parts of Chinese parasol tree tender shoots, 20~25 parts of orange peels, 10~15
Part chinaberry and 6~9 portions of peppermints, are put into mixer and are stirred, and dry, mill, and collect thing of milling；
（2）To be milled thing and glucose solution in mass ratio 1：3~5 are uniformly mixed, and are put into fermentation tank, add material of milling
The bacterium powder of amount 6~8%, ferments 1~2 day at 30~38 DEG C, collects fermenting mixture, carries out steam blasting, collects explosion thing；
（3）By explosion thing, edible fly maggot and ethanol solution in mass ratio 5：1：8 are put into ball mill and carry out ball milling, collect ball milling
Thing, is placed in standing at 45~50 DEG C, filtering, collect filtrate, rotary evaporation recycling ethanol, collect residue, by residue with
Mixed liquor in mass ratio 6：1~3 is uniformly mixed, and sterilizing, must lure liquid, spare；
（4）Count in parts by weight, take 60~63 parts of eggplant leaves, 50~55 parts of potato leafs, 26~32 parts of Commeline communis, 19~23 parts
Nettle is uniformly mixed, and dries, and crushes, and crushed material is collected, by crushed material and 1.2mol/L ethanol solutions in mass ratio 1：3 mixing,
Supercritical carbon dioxide extracting is carried out, collects extract, filtering, collects filtrate, rotary evaporation recycling ethanol, will evaporate residue
With essential oil in mass ratio 4：1 is uniformly mixed, and obtains protection liquid；
（5）By step（3）Spare lures liquid and protection liquid in mass ratio 4：3~5, bottle respectively, up to citrus Huanglong is prevented
Sick medicament.
2. the preparation method of Citrus Huanglongbing pathogen medicament is prevented according to claim 1, it is characterised in that the step（2）In
Bacterium powder is any one in yeast bacterium powder, aspergillus oryzae bacterium powder.
3. the preparation method of Citrus Huanglongbing pathogen medicament is prevented according to claim 1, it is characterised in that the step（2）In
Steam blasting condition is 120~125 DEG C, 1.6~2.0MPa of pressure of temperature, maintains 1~3min of pressure.
4. the preparation method of Citrus Huanglongbing pathogen medicament is prevented according to claim 1, it is characterised in that the step（3）In
Mixed liquor is in mass ratio 1：2：5~8, zeins, sodium alginate and 0.2mol/L sodium phosphates are uniformly mixed, to obtain the final product
Mixed liquor.
5. the preparation method of Citrus Huanglongbing pathogen medicament is prevented according to claim 1, it is characterised in that the step（4）In
Essential oil is any one in marigold essential oil, galic essential oil.
6. the preparation method of Citrus Huanglongbing pathogen medicament is prevented according to Claims 1 to 5 any one, it is characterised in that institute
State prevention Citrus Huanglongbing pathogen medicament when in use, will first lure liquid and water in mass ratio 1：13 mixing, are sprayed on mandarin tree, spray
After spilling 2~4h, then will protection liquid and water in mass ratio 1：7 mixing, are sprayed on mandarin tree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711356238.8A CN107969443A (en) | 2017-12-16 | 2017-12-16 | A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament |
Applications Claiming Priority (1)
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| CN110786159A (en) * | 2019-11-26 | 2020-02-14 | 宾川县云福农副产品加工有限责任公司 | Citrus seedling growing method |
| CN110786199A (en) * | 2019-11-26 | 2020-02-14 | 宾川县云福农副产品加工有限责任公司 | Citrus planting method |
| CN111567525A (en) * | 2020-05-08 | 2020-08-25 | 易之泰生物科技（龙岩）有限公司 | Composition and method for preventing and treating citrus greening disease |
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