CN106417412A - Biological bacterial killing agent for control of apple tree growth ring disease and preparation method thereof - Google Patents
Biological bacterial killing agent for control of apple tree growth ring disease and preparation method thereof Download PDFInfo
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- CN106417412A CN106417412A CN201610928226.7A CN201610928226A CN106417412A CN 106417412 A CN106417412 A CN 106417412A CN 201610928226 A CN201610928226 A CN 201610928226A CN 106417412 A CN106417412 A CN 106417412A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
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Abstract
The invention belongs to technical field of pesticide, in particular relates to biological bacterial killing agent for control of apple tree growth ring disease and preparation method thereof, the biological bacterial killing agent for control of apple tree growth ring disease is prepared by weight from, 13-20 parts of white mulberry bark, 10-15 parts of bitter ginseng, 6-10 parts of fructus cnidii, 7-13 parts of coptis chinensis, 6-12 parts of radix stemonae, 10-15 parts of tree peony bark, 8-15 parts of golden cypress, 9-16 parts of radix gentianae, 10-16 parts of dispersant, 3-10 parts of wetting agent, 0-5 parts of disintegrating agent and 50-80 parts of filling. The biological bactericide chemical property stays stable, the bactericide storing period lasts 2 years, and the bactericide leaves no remnant, resolves and reduces easily, causes no harm to human and animal, readily promotes and applies.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of biofungicide for preventing and treating apple tree canker and its system
Preparation Method.
Background technology
Apple tree ring spot also known as scurf, wheel stricture of vagina decayed fruit disease, are one of important diseases in apple production.Fructus Mali pumilae wheel stricture of vagina
Disease includes apple tree Trunk canker and fruit ring spot.The generation of China's ring rot of apple now becomes serious with harm mouth, no
Only harm Apple causes the underproduction, and endangers branch, causes branch ulcer, weakens tree vigo(u)r, reduces result, causes under yield
Drop, causes larger economic loss.
Chinese patent CN100431420C discloses a kind of plant disease controlling agent, and each component and its percentage by weight are respectively
For：Zhongshengmycin 1%-10%, the withered 5%-30% of saliva, copper acetate 10%-45%, auxiliary agent 1%-10%, filler 5%-83%.But, should
Patent adopts chemical pesticide, easily remains, has harm to people and animals, and resulting dosage forms aqueous suspension agent storage life is short.
Chinese patent CN105248468A discloses a kind of antibacterial of preventing and treating red Fuji apple tree ring spot, belongs to fruit tree
Techniques of pest control field.The raw material that the antibacterial includes is consisted of：Natural plants medicinal liquid, Bordeaux mixture, EM stock solution,
3% carboxymethylcellulose sodium solution, xanthan gum and water；The raw material composition of natural plants medicinal liquid therein includes：Semen Caryae Cathayensis bark,
Folium Zanthoxyli Bungeani, Folium Pini, Herba Xanthii, Pyrethrum, Flos Caryophylli, Fructus Toosendan, Fructus Toosendan, Radix Sophorae Flavescentiss.But, the patent is still using the agriculture of multiple chemistry
Medicine, easily remains, and has harm, and the plant material effective ingredient volatile oil for adopting to people and animals, and drug effect is unstable.
Content of the invention
For overcoming drawbacks described above, it is an object of the invention to provide a kind of preventing and treating apple tree ring spot biofungicide and
Its preparation method.
For achieving the above object, the present invention is adopted the following technical scheme that：
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
Cortex Mori 13-20 part, Radix Sophorae Flavescentiss 10-15 part, Fructus Cnidii 6-10 part, Rhizoma Coptidis 7-13 part, Radix Stemonae 6-12 part, Cortex Moutan 10-15
Part, Cortex Phellodendri 8-15 part, Radix Gentianae 9-16 part, dispersant 10-16 part, wetting agent 3-10 part, disintegrating agent 0-5 part and filler 50-80
Part.
Preferably, described dispersant is sodium lignin sulfonate, calcium lignosulfonate, oleic acid polyoxyethylene, dodecyl
At least one of sodium sulfate and dodecylbenzene sodium sulfonate.
Preferably, the wetting agent be di-2-ethylhexylphosphine oxide how in sodium sulfonate, naphthalene sulfonic acid-formaldehyde condensation product and methyl oleate at least
A kind of.
Preferably, the disintegrating agent is at least one of ammonium sulfate, carbamide and low-substituted hydroxypropyl cellulose.
Preferably, described filler is at least one in Kaolin, bentonite, kieselguhr and attapulgite clay.
A kind of preparation method of the biofungicide of preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）The alcohol solution dipping 10-20h of 5-10 times of weight is added in compound, then under the conditions of 50-60 DEG C
Supersound extraction 2-3 time, each 20-40min；
（3）By step（2）Middle gained extracting solution is evaporated to the 10-20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 20-30min is stirred, through comminution by gas stream,
20-30min is again stirring for, is obtained final product.
Preferably, step（1）The plant material is crushed to particle diameter for 40-80 mesh.
Preferably, step（2）Volumes of aqueous ethanol concentration >=60%.
Preferably, step（2）Supersonic frequency 20-30kHz.
Preferably, step（4）The comminution by gas stream to powder diameter is 400-600 mesh.
The positive beneficial effect of the present invention：
1. Cortex Mori of the present invention is sweet, trembles with fear, antibacterial；Radix Sophorae Flavescentiss bitter in the mouth, cold in nature, heat clearing and damp drying, parasite killing；Fructus Cnidii is pungent, bitter, temperature, the kidney warming
Tonifying YANG, dampness, wind-dispelling, parasite killing；Rhizoma Coptidis hardship, trembles with fear, resisting pathogenic microbes；The Radix Stemonae is sweet, bitter, tepor, resisting pathogenic microbes and parasite killing
Action effect is good；Cortex Mori, Radix Sophorae Flavescentiss, Fructus Cnidii, Rhizoma Coptidis and the Radix Stemonae are shared, and bactericidal effect is notable.Cortex Moutan is bitter, pungent, is slightly cold, and resists
Inflammation, antibacterial；Cortex Phellodendri hardship, trembles with fear, heat-clearing and toxic substances removing, and containing more berberine, antibacterial action is good；Radix Gentianae bitter in the mouth, cold in nature, antibacterial；Male
Cortex Moutan, Cortex Phellodendri and Radix Gentianae are shared with above-mentioned various raw materials, further enhancing the fungistatic effect of antibacterial.Above-mentioned each plant original
Material prepares wettable powder with adjuvant, and the property of medicine is stable, is kept for the phase up to 2 years, and noresidue is degradable, to person poultry harmless, can promote
Application.
2. dispersant of the present invention is sodium lignin sulfonate, calcium lignosulfonate, oleic acid polyoxyethylene, dodecane
At least one of base sodium sulfate and dodecylbenzene sodium sulfonate, biological degradation rate height, with low cost.The wetting agent is methylene
At least one of double how sodium sulfonates, naphthalene sulfonic acid-formaldehyde condensation product and methyl oleate, are sufficiently mixed uniformly to each raw material.The disintegrate
Agent is at least one of ammonium sulfate, carbamide and low-substituted hydroxypropyl cellulose, and wettable powder rapid dispersion in water is easy to spray
Spill.Described filler is at least one in Kaolin, bentonite, kieselguhr and attapulgite clay, and filler aperture is big, equal to raw material
Even dispersion, is conducive to giving full play to for drug effect.
3. each plant material extracts active ingredients of the present invention are complete, and effective ingredient is not volatile, efficacy stability, low cost
Honest and clean.
Specific embodiment
With reference to some specific embodiments, the present invention is further described.
Embodiment 1
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
13 parts of Cortex Mori, 10 parts of Radix Sophorae Flavescentiss, 6 parts of Fructus Cnidii, 7 parts of Rhizoma Coptidis, 6 parts of the Radix Stemonae, 10 parts of Cortex Moutan, 8 parts of Cortex Phellodendri, Radix Gentianae 9
Part, 10 parts of dispersant, 3 parts of wetting agent and 50 parts of filler.
The dispersant is sodium lignin sulfonate.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate.
Described filler is Kaolin.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 50 mesh, mix homogeneously；
（2）By step（1）60% alcohol solution dipping 15h of 6 times of weight is added in compound, and then ultrasound is carried under the conditions of 55 DEG C
Take 3 times, each 20min；Supersonic frequency 30kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 15% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 500 mesh, again
Stirring 30min, obtains final product.
Embodiment 2
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
14 parts of Cortex Mori, 11 parts of Radix Sophorae Flavescentiss, 7 parts of Fructus Cnidii, 8 parts of Rhizoma Coptidis, 7 parts of the Radix Stemonae, 11 parts of Cortex Moutan, 9 parts of Cortex Phellodendri, Radix Gentianae 10
Part, 11 parts of dispersant, 4 parts of wetting agent, 1 part of disintegrating agent and 50 parts of filler.
The dispersant is calcium lignosulfonate.
The wetting agent is naphthalene sulfonic acid-formaldehyde condensation product.
The disintegrating agent is ammonium sulfate.
Described filler is bentonite.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 60 mesh, mix homogeneously；
（2）By step（1）75% alcohol solution dipping 20h of 8 times of weight is added in compound, and then ultrasound is carried under the conditions of 60 DEG C
Take 3 times, each 30min；Supersonic frequency 25kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 600
Mesh, is again stirring for 25min, obtains final product.
Embodiment 3
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
15 parts of Cortex Mori, 12 parts of Radix Sophorae Flavescentiss, 7.5 parts of Fructus Cnidii, 9 parts of Rhizoma Coptidis, 8 parts of the Radix Stemonae, 12 parts of Cortex Moutan, 10 parts of Cortex Phellodendri, Radix Gentianae
11 parts of grass, 12 parts of dispersant, 5 parts of wetting agent, 2 parts of disintegrating agent and 60 parts of filler.
The dispersant is oleic acid polyoxyethylene.
The wetting agent is methyl oleate.
The disintegrating agent is carbamide and low-substituted hydroxypropyl cellulose, and both amount ratios are 1:1.
Described filler is kieselguhr.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed to 40 mesh, mix homogeneously；
（2）By step（1）80% alcohol solution dipping 15h of 10 times of weight is added in compound, and then ultrasound is carried under the conditions of 55 DEG C
Take 2 times, each 40min；Supersonic frequency 30kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 15% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 20min is stirred, through comminution by gas stream to 500
Mesh, is again stirring for 20min, obtains final product.
Embodiment 4
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
16 parts of Cortex Mori, 13 parts of Radix Sophorae Flavescentiss, 8 parts of Fructus Cnidii, 10 parts of Rhizoma Coptidis, 9 parts of the Radix Stemonae, 13 parts of Cortex Moutan, 11 parts of Cortex Phellodendri, Radix Gentianae
12 parts, 13 parts of dispersant, 6 parts of wetting agent, 3 parts of disintegrating agent and 70 parts of filler.
The dispersant is sodium lauryl sulphate.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate.
The disintegrating agent is ammonium sulfate.
Described filler is attapulgite clay.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed to 80 mesh, mix homogeneously；
（2）By step（1）80% alcohol solution dipping 20h of 10 times of weight is added in compound, then ultrasound under the conditions of 55 DEG C
Extract 3 times, each 40min；Supersonic frequency 20kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 20min is stirred, through comminution by gas stream to 500
Mesh, is again stirring for 25min, obtains final product.
Embodiment 5
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
16 parts of Cortex Mori, 13 parts of Radix Sophorae Flavescentiss, 8 parts of Fructus Cnidii, 10 parts of Rhizoma Coptidis, 9 parts of the Radix Stemonae, 13 parts of Cortex Moutan, 11 parts of Cortex Phellodendri, Radix Gentianae
12 parts, 5 parts of the Rhizoma Anemarrhenae, 13 parts of dispersant, 6 parts of wetting agent, 3 parts of disintegrating agent and 70 parts of filler.
The present embodiment adds the Rhizoma Anemarrhenae, and the Rhizoma Anemarrhenae is bitter, sweet, trembles with fear, and resisting pathogenic microbes act on, and share with other each raw materials, enter one
Step enhances the cold and cool property of medicine of antibacterial, is easy to sterilization.
The dispersant is sodium lauryl sulphate.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate.
The disintegrating agent is ammonium sulfate.
Described filler is attapulgite clay.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot is shown in embodiment 4.
Embodiment 6
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
17 parts of Cortex Mori, 14 parts of Radix Sophorae Flavescentiss, 9.5 parts of Fructus Cnidii, 11 parts of Rhizoma Coptidis, 10 parts of the Radix Stemonae, 14 parts of Cortex Moutan, 12 parts of Cortex Phellodendri, dragon
13 parts of Radix Gentianae, 14 parts of dispersant, 7 parts of wetting agent, 4 parts of disintegrating agent and 75 parts of filler.
The dispersant is dodecylbenzene sodium sulfonate.
The wetting agent is methyl oleate.
The disintegrating agent is low-substituted hydroxypropyl cellulose.
Described filler is Kaolin and attapulgite clay, and both amount ratios are 1:1.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed to 40 mesh, mix homogeneously；
（2）By step（1）90% alcohol solution dipping 10h of 8 times of weight is added in compound, and then ultrasound is carried under the conditions of 50 DEG C
Take 3 times, each 30min；Supersonic frequency 25kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 15% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 25min is stirred, through comminution by gas stream to 400
Mesh, is again stirring for 30min, obtains final product.
Embodiment 7
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
19 parts of Cortex Mori, 14.5 parts of Radix Sophorae Flavescentiss, 9.5 parts of Fructus Cnidii, 12 parts of Rhizoma Coptidis, 11 parts of the Radix Stemonae, 14.5 parts of Cortex Moutan, Cortex Phellodendri 14
Part, 14 parts of Radix Gentianae, 15 parts of dispersant, 8 parts of wetting agent, 4.5 parts of disintegrating agent and 80 parts of filler.
The dispersant is sodium lignin sulfonate and sodium lauryl sulphate, and both weight ratios are 1:1.
The wetting agent is naphthalene sulfonic acid-formaldehyde condensation product.
The disintegrating agent is carbamide.
Described filler is kieselguhr.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed to 60 mesh, mix homogeneously；
（2）By step（1）75% alcohol solution dipping 20h of 5 times of weight is added in compound, and then ultrasound is carried under the conditions of 55 DEG C
Take 2 times, each 20min；Supersonic frequency 30kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 10% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 600
Mesh, is again stirring for 20min, obtains final product.
Embodiment 8
A kind of biofungicide of preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
20 parts of Cortex Mori, 15 parts of Radix Sophorae Flavescentiss, 10 parts of Fructus Cnidii, 13 parts of Rhizoma Coptidis, 12 parts of the Radix Stemonae, 15 parts of Cortex Moutan, 15 parts of Cortex Phellodendri, Radix Gentianae
16 parts of grass, 20 parts of dispersant, 10 parts of wetting agent, 5 parts of disintegrating agent and 80 parts of filler.
The dispersant is dodecylbenzene sodium sulfonate.
The wetting agent is di-2-ethylhexylphosphine oxide how sodium sulfonate and methyl oleate, both weight ratios be:1.
The disintegrating agent is low-substituted hydroxypropyl cellulose.
Described filler is bentonite.
The preparation method of the biofungicide of above-mentioned preventing and treating apple tree ring spot, comprises the following steps：
（1）The various plant materials of the parts by weight are weighed, crushes 50 mesh, mix homogeneously；
（2）By step（1）60% alcohol solution dipping 20h of 6 times of weight is added in compound, and then ultrasound is carried under the conditions of 60 DEG C
Take 3 times, each 20min；Supersonic frequency 30kHz；
（3）By step（2）Middle gained extracting solution is evaporated to the 15% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent and filler are added in plant extract, 30min is stirred, through comminution by gas stream to 400 mesh, again
Stirring 30min, obtains final product.
Comparative example 1
The biofungicide of present invention preventing and treating apple tree ring spot is substantially the same manner as Example 4, and somewhat different is：
The biofungicide of the preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
13 parts of Radix Sophorae Flavescentiss, 8 parts of Fructus Cnidii, 10 parts of Rhizoma Coptidis, 9 parts of the Radix Stemonae, 11 parts of Cortex Phellodendri, 12 parts of Radix Gentianae, 13 parts of dispersant, wetting agent
6 parts, 3 parts of disintegrating agent and 70 parts of filler.
Comparative example 2
The biofungicide of present invention preventing and treating apple tree ring spot is substantially the same manner as Example 4, and somewhat different is：The preventing and treating
The biofungicide of apple tree ring spot, the raw material including following parts by weight is made：
16 parts of Cortex Mori, 8 parts of Fructus Cnidii, 10 parts of Rhizoma Coptidis, 13 parts of Cortex Moutan, 11 parts of Cortex Phellodendri, 12 parts of Radix Gentianae, 13 parts of dispersant,
6 parts of wetting agent, 3 parts of disintegrating agent and 70 parts of filler.
Comparative example 3
The biofungicide of present invention preventing and treating apple tree ring spot is substantially the same manner as Example 4, and somewhat different is：
The biofungicide of the preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
16 parts of Cortex Mori, 13 parts of Radix Sophorae Flavescentiss, 10 parts of Rhizoma Coptidis, 9 parts of the Radix Stemonae, 13 parts of Cortex Moutan, 11 parts of Cortex Phellodendri, 13 parts of dispersant, moistening
6 parts of agent, 3 parts of disintegrating agent and 70 parts of filler.
Comparative example 4
The biofungicide of present invention preventing and treating apple tree ring spot is substantially the same manner as Example 4, and somewhat different is：
The biofungicide of the preventing and treating apple tree ring spot, the raw material including following parts by weight is made：
16 parts of Cortex Mori, 13 parts of Radix Sophorae Flavescentiss, 8 parts of Fructus Cnidii, 9 parts of the Radix Stemonae, 13 parts of Cortex Moutan, 12 parts of Radix Gentianae, 13 parts of dispersant, profit
6 parts of humectant, 3 parts of disintegrating agent and 70 parts of filler.
Application test
Test method：By each embodiment medicament is determined using growth rate method, the suppression of apple tree ring spot mycelial growth is made
With, experimental group prevents and treats the biofungicide of apple tree ring spot using embodiment of the present invention 1-8, matched group 1-4, and clear water dilutes,
Safe matched group（CK）Using aquesterilisa, reagent agent is added in 40-50 DEG C of PDA culture medium, is sufficiently mixed, in culture dish
In be paved into content for the malicious flat board of 10% band, often process 3 repetitions, by the pathogenic fungi pure culture biscuits involvng inoculation of a diameter of 4mm in flat board just
In, 23 ± 1 DEG C, cultivate under dark condition, 3d measures each colony diameter, and experimental result is shown in Table 1.
Colony diameter（mm）=measurement diameter -4；
Mycelial growth inhibition rate=（Control colony diameter-process colony diameter）/ control colony diameter × 100%.
Prevention effect of 1 embodiment of the present invention 1-8 biofungicide of table to apple tree ring spot
As shown in Table 1, embodiment of the present invention 1-8 biofungicide is to the suppression ratio of apple tree ring spot mycelia up to more than 80%,
Prevention effect is notable, lacks Cortex Mori and Cortex Moutan in 1 raw material of comparative example, lack in 2 raw material of comparative example Radix Sophorae Flavescentiss and
The Radix Stemonae, lacks Fructus Cnidii and Radix Gentianae in 3 raw material of comparative example, comparative example 4 lacks Rhizoma Coptidis and Cortex Phellodendri, and prevention effect is then
It is decreased obviously, illustrates that each raw material synergistic action effect of the present invention is notable.
Claims (10)
1. a kind of preventing and treating apple tree ring spot biofungicide, it is characterised in that the raw material including following parts by weight is made：
Cortex Mori 13-20 part, Radix Sophorae Flavescentiss 10-15 part, Fructus Cnidii 6-10 part, Rhizoma Coptidis 7-13 part, Radix Stemonae 6-12 part, Cortex Moutan 10-15
Part, Cortex Phellodendri 8-15 part, Radix Gentianae 9-16 part, dispersant 10-16 part, wetting agent 3-10 part, disintegrating agent 0-5 part and filler 50-80
Part.
2. the biofungicide of preventing and treating apple tree ring spot according to claim 1, it is characterised in that the dispersant is
In sodium lignin sulfonate, calcium lignosulfonate, oleic acid polyoxyethylene, sodium lauryl sulphate and dodecylbenzene sodium sulfonate extremely
Few one kind.
3. the biofungicide of preventing and treating apple tree ring spot according to claim 1, it is characterised in that the wetting agent is
Di-2-ethylhexylphosphine oxide how at least one of sodium sulfonate, naphthalene sulfonic acid-formaldehyde condensation product and methyl oleate.
4. the biofungicide of preventing and treating apple tree ring spot according to claim 1, it is characterised in that the disintegrating agent is
At least one of ammonium sulfate, carbamide and low-substituted hydroxypropyl cellulose.
5. according to claim 1 preventing and treating apple tree ring spot biofungicide, it is characterised in that described filler for height
At least one in ridge soil, bentonite, kieselguhr and attapulgite clay.
6. the preparation method of the biofungicide of the preventing and treating apple tree ring spot described in a kind of any one of claim 1-5, which is special
Levy and be, comprise the following steps：
（1）The various plant materials of the parts by weight are weighed, is crushed, mix homogeneously；
（2）By step（1）The alcohol solution dipping 10-20h of 5-10 times of weight is added in compound, then under the conditions of 50-60 DEG C
Supersound extraction 2-3 time, each 20-40min；
（3）By step（2）Middle gained extracting solution is evaporated to the 10-20% of original solution volume, dries, obtains plant extract；
（4）Dispersant, wetting agent, disintegrating agent and filler are added in plant extract, 20-30min is stirred, through comminution by gas stream,
20-30min is again stirring for, is obtained final product.
7. according to claim 6 preventing and treating apple tree ring spot biofungicide preparation method, it is characterised in that step
Suddenly（1）The plant material is crushed to particle diameter for 40-80 mesh.
8. according to claim 6 preventing and treating apple tree ring spot biofungicide preparation method, it is characterised in that step
Suddenly（2）Volumes of aqueous ethanol concentration >=60%.
9. according to claim 6 preventing and treating apple tree ring spot biofungicide preparation method, it is characterised in that step
Suddenly（2）Supersonic frequency 20-30kHz.
10. according to claim 6 preventing and treating apple tree ring spot biofungicide preparation method, it is characterised in that
Step（4）The comminution by gas stream to powder diameter is 400-600 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610928226.7A CN106417412A (en) | 2016-10-31 | 2016-10-31 | Biological bacterial killing agent for control of apple tree growth ring disease and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610928226.7A CN106417412A (en) | 2016-10-31 | 2016-10-31 | Biological bacterial killing agent for control of apple tree growth ring disease and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN106417412A true CN106417412A (en) | 2017-02-22 |
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| CN201610928226.7A Pending CN106417412A (en) | 2016-10-31 | 2016-10-31 | Biological bacterial killing agent for control of apple tree growth ring disease and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107810756A (en) * | 2017-11-07 | 2018-03-20 | 广西吉朋投资有限公司 | A kind of prevention and controls of apple tree ring spot |
| CN115226734A (en) * | 2022-08-25 | 2022-10-25 | 渭南职业技术学院 | Walnut green seedcase and Chinese cabbage composite agricultural enzyme composition as well as preparation method and application thereof |
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| CN101156606A (en) * | 2007-11-20 | 2008-04-09 | 史建生 | A plants antiseptic as well as preparing method and application |
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| CN101156606A (en) * | 2007-11-20 | 2008-04-09 | 史建生 | A plants antiseptic as well as preparing method and application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107810756A (en) * | 2017-11-07 | 2018-03-20 | 广西吉朋投资有限公司 | A kind of prevention and controls of apple tree ring spot |
| CN115226734A (en) * | 2022-08-25 | 2022-10-25 | 渭南职业技术学院 | Walnut green seedcase and Chinese cabbage composite agricultural enzyme composition as well as preparation method and application thereof |
| CN115226734B (en) * | 2022-08-25 | 2023-07-25 | 渭南职业技术学院 | Walnut green seedcase and jiuxin vegetable composite agricultural enzyme composition and preparation method and application thereof |
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