CN101961019B - Plant source bactericide and application thereof - Google Patents
Plant source bactericide and application thereof Download PDFInfo
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- CN101961019B CN101961019B CN 201010523869 CN201010523869A CN101961019B CN 101961019 B CN101961019 B CN 101961019B CN 201010523869 CN201010523869 CN 201010523869 CN 201010523869 A CN201010523869 A CN 201010523869A CN 101961019 B CN101961019 B CN 101961019B
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- osthole
- jamaicin
- aqua
- plant source
- active ingredients
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- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 9
- 239000003899 bactericide agent Substances 0.000 title abstract description 7
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical compound C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 claims abstract description 55
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 claims abstract description 54
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical group CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims abstract description 17
- 201000010099 disease Diseases 0.000 claims abstract description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 15
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical group [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 14
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 14
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 14
- 239000004094 surface-active agent Substances 0.000 claims abstract description 12
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 9
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 9
- 239000003755 preservative agent Substances 0.000 claims abstract description 6
- 230000002335 preservative effect Effects 0.000 claims abstract description 6
- WITLAWYGGVAFLU-UHFFFAOYSA-N 3-(6-methoxy-1,3-benzodioxol-5-yl)-8,8-dimethylpyrano[2,3-f]chromen-4-one Chemical compound C1=CC(C)(C)OC2=CC=C(C(C(C3=CC=4OCOC=4C=C3OC)=CO3)=O)C3=C21 WITLAWYGGVAFLU-UHFFFAOYSA-N 0.000 claims description 110
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000000855 fungicidal effect Effects 0.000 claims description 14
- 239000000417 fungicide Substances 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 11
- 239000003960 organic solvent Substances 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 abstract description 32
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical group CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 abstract description 14
- 241000221785 Erysiphales Species 0.000 abstract description 13
- 230000002195 synergetic effect Effects 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 abstract description 5
- 229940093265 berberine Drugs 0.000 abstract description 5
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 abstract description 5
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- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
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- 244000247747 Coptis groenlandica Species 0.000 description 3
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a plant source bactericide and application thereof. The plant source bactericide is prepared by mixing two active ingredients, a surfactant NP-100, a penetrating agent, an antioxidant, a preservative and a solvent, wherein the two active ingredients are berberine and osthole in a ratio of the berberine to the osthole of 1:20 to 20:1; the penetrating agent is azone; the antioxidant is 2,6-di-tert-butyl-p-cresol; and the preservative is sodium benzoate. The plant source bactericide is a broad spectrum bactericide and is mainly applied to preventing and controlling important diseases such as powdery mildew, plague, gray mold and the like. The plant source bactericide has synergistic effect after the two active ingredients are mixed, is insusceptible to resistance generation, has low toxicity for human and livestock, is safe to environment and has wide application to production of pollution-free and organic agriculture.
Description
Technical field
The present invention relates to biological pesticide technical field, be specifically related to a kind of botanical fungicide and application thereof.This bactericide is mainly used in the control of the important plant diseases such as powdery mildew, epidemic disease and gray mold, can be widely used in nuisanceless and organic farming production.
Background technology
Agricultural chemicals is to ensure agricultural production and the indispensable important means of production of grain security, but since the sixties in last century, the long-term use of a large amount of chemical pesticides has caused serious negative effect to environment and human and livestock health.Although country pays much attention to the security management work of agricultural chemicals in recent years, successively prohibit, limited the use of tens kinds of high poison, persistent pesticide, but because the exceed standard pollution of dining table that causes and the poisoning of initiation of residue of pesticide still happens occasionally, the development of people's health and agricultural has been caused harmful effect.The agricultural product quality and safety management has become the important component part of state food safety management, is the emphasis of current attention from government, the focus of social concerns.Simultaneously, the pesticide quality safety problem also is the key factor that affects China's present stage Agricultural Competitive.China is as WTO member state and export of farm produce big country, recent years since persticide residue exceed standard, with the trade dispute of the states such as Europe, the U.S. and Japan continuous, greatly affected the export trade of agricultural product, food.
Population is growing, arable area reduces increasingly, although transgenic technology and Resistant breeding technique can alleviate the generation of disease pest and weed to a certain extent, but still can not replace the critical positions of agricultural chemicals.Therefore, for Guarantee Grain Production safety, must use agricultural chemicals to carry out " insect population is taken grain by force ".Simultaneously, in order to ensure the safety that agricultural chemicals uses, also must vigorously promote the use environmentally safe, to the biopesticide of people and animals' low toxicity.As the important member of biopesticide, although the botanical pesticide applicating history is long, because the past is low to the availability of botanical pesticide active ingredient, usually, effect slow because of effect is unstable can't use by spread.Therefore, be badly in need of better, the stay-in-grade pollution-free plant source pesticide product of some action effects of exploitation in the production.
Jamaicin (Berberine) claim again berberine, is a kind of common morphinane alkaloid, molecular formula C
20H
18NO
4It is present in many plants of ten genus of four sections such as Berberidaceae, such as the coptis, giant knotweed etc.Jamaicin is pharmaceutically of many uses, has a broad antifungal spectrum, external to multiple Grain-positive and the equal tool bacteriostasis of negative bacterium, wherein hemolytic streptococcus gold Portugal bacterium, comma bacillus, meningococcus, shigella, typhoid bacillus, diphtheria bacillus etc. there is stronger inhibitory action, antibacterial during low concentration, sterilization during high concentration.In addition, infected by influenza, Amoeba, Leptospira, some dermatophyte also have certain inhibitory action.But medical research is indicated shigella dysenteriae, hemolytic streptococcus, golden Portugal bacterium etc. and very easily jamaicin is produced drug resistance.Have at present in addition 0.5% jamaicin aqua is used for the diseases such as tomato gray mould, cucumber white powder, capsicum epidemic disease, tomato leaf mold in the agricultural chemicals registration control.
Osthole (Osthol) is the main active ingredient of samphire frutus cnidii fruit, it also is a kind of important medical material, has broad-spectrum antibacterial action, beard and hair, toe, crissum tinea bacterium there is stronger inhibitory action, Trichomonas vaginalis also there is inhibitory action, and has antimycotic, resistance attitude, the effect such as antitumor.Registered control for powdery mildew of cucumber and insect pest on agricultural chemicals.
Although the registration use in agricultural chemicals of jamaicin and Osthole is compared with the conventional chemical agricultural chemicals, still there is the problem that effect is slow, preventive effect is low, the product on market is compared and is lacked competitiveness.Trace it to its cause, mainly be: the one, active ingredient is single, and preventive effect is limited, and germ develops immunity to drugs easily after long-term the use; The 2nd, preparation processing technology existing problems, so that the active ingredient of these natural botanical components is unstable, the plant absorbing availability is low.Jamaicin is two kinds of fully identical, not close compounds of chemical constitution with Osthole, therefore the mechanism of action is different, can avoid and delay germ after the present invention is mixed them develops immunity to drugs to these two kinds of active ingredients, simultaneously, after being mixed, these two kinds of active ingredients also have significant synergies, not only control efficiency can be improved, the consumption of these resource-type active ingredients can also be reduced.In addition, the present invention has also added antioxidant to increase the stability of product in product; Added short penetrant azone to improve crop to absorption and the utilization ratio of active ingredient.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of botanical fungicide,
Jamaicin and these two kinds of active ingredients of Osthole are mixed and add antioxidant and short bleeding agent, reach the purpose that improves product stability and control efficiency, reduce effective ingredient consumption, delaying drug resistance economizes on resources.
Above-mentioned technical purpose is achieved through the following technical solutions, a kind of botanical fungicide, and the component following by content forms:
Jamaicin 0.4~8wt%;
Osthole 0.2~8wt%;
Azone 3wt%;
Surfactant 10wt%;
Antioxidant 0.005wt%;
Preservative 0.5wt%;
Organic solvent 24.5~73.6wt%;
Surplus is water.
This is mixed in the botanical fungicide, the mass ratio of jamaicin and Osthole is controlled at 1:20~20:1, these two kinds of active ingredients can (can directly be bought crude extract for the extract at any position of corresponding plant, the also available coptis, frutus cnidii extracts, extraction process is: vegetable material is pulverized, cross 20~40 mesh sieves, then mix in the ratio of 40~50wt% ethanol of the corresponding 6-10L of every kg feed material powder and infiltrate dissolving, refluxing extraction, the extract diacolation reclaims and gets final product), it also can be the smart low content raffinate that extracts in the process of medicine, but need to guarantee content and the proportioning of above-mentioned two kinds of active ingredients, then add successively organic solvent (ethanol, methyl alcohol etc.), surfactant (non-ionic surface active agent, such as ethoxylated nonylphenol class non-ionic surface active agent NP100, NP200 etc.), azone, antioxidant (2, the 6-BHT, claim again BHT or antioxidant 264), preservative sodium benzoate and water, stirring to obtain botanical fungicide of the present invention.
Compared with prior art, advantage of the present invention and beneficial effect are as follows:
1, is mixed by the botanical pesticide active ingredient with two kinds of different active ingredients, increases on the one hand and render a service, reduce the consumption of active ingredient, saved resource; On the other hand, stability and the control efficiency of botanical fungicide have been improved by in preparation, adding penetrating agent (azone), antioxidant etc.; Simultaneously, because the chemical constitution of two active ingredients is fully different, have different mechanism of action, also can avoid or delay phytopathogen after therefore being mixed and produce resistance;
2, the control of botanical fungicide of the present invention spectrum is wider, not only effective to high fungal diseases such as powdery mildew, gray molds, still effective to oomycetes diseases such as phytophthora root rots simultaneously;
Therefore 3, two kinds of active ingredients derive from natural frond, and all pharmaceutically are being widely used, and are safe to people and animals' health and environment, meet the standard of efficient, noresidue, pollution-free pesticide; In addition, this agricultural chemicals also can take full advantage of discarded plant material (such as the leaf in the coptis process, fibrous root Chang Zuowei garbage disposal) and the factory's residue waste liquid in the medical process of manufacture, turns waste into wealth.
Embodiment
Below in conjunction with specific embodiment the present invention is done further and to elaborate.
1. purposes and scope
The present invention is mainly used in the control of the plant diseases such as powdery mildew of cucumber, Phytophthora capsici, graw mold of tomato, at premorbid or their early stage use disease is had better control efficiency.
2. Toxicity Determination and prescription screening
At first take the Phytophthora capsici germ as target, carry out the proportioning screening of jamaicin and Osthole.Its principle is: adopt the mycelial growth rate method mixture that water agar is measured these two kinds of single doses and different proportionings thereof to the establishment of Phytophthora capsici germ mycelial growth in concentration EC50 value, then adopt the abundant co-toxicity coefficient method of Sun Yun (SUN Y P, JOHNSON E R .Analysis of Joint Action of Insecticides Against House Flies. Journal of Economic Entomology, 1960, the 53:887-892.) mode of application of analysis mixture preparation:
The theoretical toxicity index TTI of mixture preparation
=∑ (toxicity index of certain medicine * mass percent of this pharmaceutically active ingredient in mixture)
The theoretical toxicity index of the actual toxicity index of co-toxicity coefficient CTC=(mixture of mixture/mixture) * 100
Wherein: CTC 〉=120 o'clock are synergistic effect; CTC≤80 o'clock are antagonism; 80<CTC<120 o'clock are summation action.
Then select the most obvious scheme that is mixed of synergistic effect again in the activity of indoor measurement to botrytis cinerea, powdery mildew of cucumber bacterium, wherein because the powdery mildew of cucumber bacterium is obligatory parasitic bacteria, the stripped cotyledon method of employing (Yang Xiaojun etc. medicament compares the biological activity determination method of powdery mildew of cucumber bacterium. hubei agricultural science .2004, (3): 71-74) measure, the results are shown in Table 1 and table 2.
The mixture preparation of table 1 jamaicin and Osthole acts on the formula indoor screening of Phytophthora capsici germ Result of the test shows, the co-toxicity coefficient of the mass ratio of jamaicin and Osthole between 1:20 ~ 20:1 is all greater than 120, explanation shows synergistic effect after both are mixed in this proportion, co-toxicity coefficient when its Berberine and Osthole are mixed in the ratio of 2:1 is maximum, be 167, synergistic effect is the most remarkable.
Table 2 jamaicin and the Osthole 2:1 mass ratio rear activity to other germs that is mixed
As shown in Table 2, jamaicin and Osthole are respectively 32.52 μ g/mL and 49.28 μ g/mL with the EC50 value that mass ratio 2:1 is mixed to powdery mildew of cucumber bacterium and botrytis cinerea, all show greater activity.The above results all is the experiments that come from vitro, and the effect of practical application is still needed will carry out in the land for growing field crops to test and carried out the preventive effect checking.
3. field control effect
Embodiment 1: 0.6% jamaicin Osthole aqua (2:1), its prescription is as follows:
Jamaicin 0.4wt%;
Osthole 0.2wt %;
Azone 3wt %;
NP-100 10wt%;
BHT 0.005wt %;
Sodium Benzoate 0.5wt%;
Ethanol 33.4wt %;
Surplus is water.
The preparation method is as follows: at first take by weighing 20g 20wt% jamaicin ethanol extract and 20g 10wt% Osthole ethanol extract, both are mixed (containing altogether 34g ethanol in the extract), then add successively ethanol 300g, surfactant (NP-100) 100g, azone 30g, BHT 0.05g, Sodium Benzoate 5g and 525g water, stirring gets final product.
Carry out this 0.6% jamaicin Osthole aqua (2:1) in Hunan and Anhui respectively in 2009 and prevented and treated the field control effectiveness test of powdery mildew of cucumber, and with the 1%(mass percent, percentage in the aqua that hereinafter occurs is mass percent) (preparation process of all contrast medicaments is identical with test medicine for contrast with 1% Osthole aqua for the jamaicin aqua, except azone, NP-100, BHT and Sodium Benzoate content immobilize, beyond test example is identical, active ingredient, the content of second alcohol and water changes according to the addition of active ingredient, guaranteeing in total content 100% and the active constituent content situation for setting value, the ratio of second alcohol and water need not fixed, and following examples together).The results are shown in Table 3, although the active ingredient total content of 0.6% jamaicin Osthole aqua is lower than jamaicin and Osthole single dose, control efficiency is suitable, illustrates to have synergistic effect after being mixed, and can reduce the consumption of active ingredient, has saved resource.
Table 3 0.6% jamaicin Osthole aqua to the powdery mildew of cucumber medicine after 14 days preventive effects (%)
Embodiment 2: 8.4% jamaicin Osthole aqua (20:1), its prescription is as follows:
Jamaicin 8wt%;
Osthole 0.4wt%;
Azone 3wt%;
NP-100 10wt%;
BHT 0.005wt%;
Sodium Benzoate 0.5wt%;
Ethanol 35.6wt%;
Surplus is water.
The preparation method is as follows: at first take by weighing 400g 20wt% jamaicin ethanol extract and 40g 10wt% Osthole ethanol extract, both are mixed, then add successively surfactant (NP-100) 100g, azone 30g, BHT 0.05g, Sodium Benzoate 5g and 425g water, stirring gets final product.
Carried out respectively the field control effectiveness test of this 8.4% jamaicin Osthole aqua (20:1) control capsicum epidemic disease in 2009 in Jiangsu and Hubei, and take 10% jamaicin aqua and 10% Osthole aqua as contrast.The results are shown in Table 4, although the active ingredient total content of 8.4% jamaicin Osthole aqua is a little less than jamaicin and Osthole single dose, control efficiency is better than single dose in the situation of the identical multiple of dilution, illustrates to have synergistic effect after being mixed, can reduce the consumption of active ingredient, save resource.
Table 4 8.4% jamaicin Osthole aqua to the capsicum epidemic disease medicine after 14 days preventive effects (%)
Embodiment 3: 8.4% jamaicin Osthole aqua (1:20), its prescription is as follows:
Jamaicin 0.4wt%;
Osthole 8wt%;
Azone 3wt%;
NP-100 10wt%;
BHT 0.005wt%;
Sodium Benzoate 0.5wt%;
Ethanol 73.6wt%;
Surplus is water.
The preparation method is as follows: at first take by weighing 20g 20wt% jamaicin ethanol extract and 800g 10wt% Osthole ethanol extract, both are mixed, then add successively surfactant (NP-100) 100g, azone 30 g, BHT 0.05g, Sodium Benzoate 5g and water 45g, stirring gets final product.
Carry out this 8.4% jamaicin Osthole aqua (1:20) in Jiangsu and Hubei respectively in 2009 and prevented and treated the field control effectiveness test of powdery mildew of cucumber, and take 10% jamaicin aqua and 10% Osthole aqua as contrast.Table sees Table 5 as a result, although the active ingredient total content of 8.4% jamaicin Osthole aqua is lower than jamaicin and Osthole single dose, control efficiency is a little more than single dose, illustrates to have synergistic effect after being mixed, and can reduce the consumption of active ingredient, has saved resource.
Table 5 8.4% jamaicin Osthole aqua to the capsicum epidemic disease medicine after 14 days preventive effects (%)
Embodiment 4: 5.5% jamaicin Osthole aqua (10:1), its prescription is as follows:
Jamaicin 5wt%;
Osthole 0.5wt%;
Azone 3wt%;
NP-100 10wt%;
BHT 0.005wt%;
Sodium Benzoate 0.5wt%;
Ethanol 24.5wt%;
Surplus is water.
The preparation method is as follows: at first take by weighing 250g 20wt% jamaicin ethanol extract and 50g 10wt% Osthole ethanol extract, both are mixed, then add successively surfactant (NP-100) 100g, azone 30g, BHT 0.05g, Sodium Benzoate 5g and 565g water, stirring gets final product.
Carried out respectively the field control effectiveness test of this 5.5% jamaicin Osthole aqua (10:1) control capsicum epidemic disease in 2009 in Hubei and Jiangsu, and take 10% jamaicin aqua and 10% Osthole aqua as contrast.The results are shown in Table 6, although the active ingredient total content of 5.5% jamaicin Osthole aqua is starkly lower than jamaicin and Osthole single dose, in the identical situation of extension rate, its control efficiency is suitable, illustrate to have synergistic effect after being mixed, can reduce the consumption of active ingredient, saved resource.
Table 6 5.5% jamaicin Osthole aqua to the capsicum epidemic disease medicine after 14 days preventive effects (%)
Embodiment 5: 2% jamaicin Osthole aqua (1:1), its prescription is as follows:
Jamaicin 1wt%;
Osthole 1wt%;
Azone 3wt%;
NP-100 10wt%;
BHT 0.005wt%;
Sodium Benzoate 0.5wt%;
Ethanol 33.0wt%;
Surplus is water.
The preparation method is as follows: at first take by weighing 50g 20wt% jamaicin ethanol extract and 100g 10% Osthole ethanol extract and mix, then add successively 200g ethanol, surfactant (NP-100) 100g, azone 30g, BHT 0.05g, Sodium Benzoate 5g and 515g water, stirring gets final product.
Carried out respectively the field control effectiveness test of this 2% jamaicin Osthole aqua (1:1) control graw mold of tomato in 2009 in Hunan and Anhui, and take 2% jamaicin aqua and 2% Osthole aqua as contrast.The results are shown in Table 7, although the active ingredient total content of 2% jamaicin Osthole aqua is identical with the Osthole single dose with jamaicin, control efficiency is better than single dose in the situation of the identical multiple of dilution, illustrates to have synergistic effect after being mixed, can reduce the consumption of active ingredient, save resource.
Table 7 2% jamaicin Osthole aqua to the powdery mildew of cucumber medicine after 14 days preventive effects (%)
Claims (6)
1. botanical fungicide is characterized in that composition and content are as follows:
Jamaicin 0.4~8wt%;
Osthole 0.2~8wt%;
Azone 3wt%;
Surfactant 10wt%;
Antioxidant 0.005wt%;
Preservative 0.5wt%;
Organic solvent 24.5~73.6wt%;
Surplus is water.
2. a kind of botanical fungicide as claimed in claim 1, it is characterized in that: described surfactant is NP-100.
3. a kind of botanical fungicide as claimed in claim 1, it is characterized in that: described antioxidant is BHT.
4. a kind of botanical fungicide as claimed in claim 1, it is characterized in that: described preservative is Sodium Benzoate.
5. a kind of botanical fungicide as claimed in claim 1, it is characterized in that: described organic solvent is ethanol.
6. such as the application of arbitrary described botanical fungicide in control of plant disease among the claim 1-5.
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CN102894002B (en) * | 2012-11-05 | 2013-11-20 | 山东省烟台市农业科学研究院 | Compound bactericide containing berberine and polyoxins and application thereof |
CN103444729A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Bactericidal pharmaceutical composition and applications thereof |
CN105104391B (en) * | 2015-08-26 | 2017-11-28 | 广西大学 | A kind of bactericidal composition containing jamaicin and copper 8-hydroxyquinolinate |
CN105076163B (en) * | 2015-08-26 | 2018-01-05 | 广西大学 | New application of the jamaicin as disinfectant use in agriculture |
CN106106529A (en) * | 2016-06-20 | 2016-11-16 | 河南中天恒信生物化学科技有限公司 | A kind of new type water rice field compound disinfectant and application thereof |
CN106538624A (en) * | 2016-10-31 | 2017-03-29 | 福建省农业科学院植物保护研究所 | A kind of coptis bactericide and its application in preventing and treating crop epidemic disease |
CN115886016B (en) * | 2022-12-07 | 2024-04-30 | 广西壮族自治区农业科学院 | Composition containing berberine |
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US6753305B2 (en) * | 2000-04-14 | 2004-06-22 | The Procter & Gamble Company | Process for disinfecting a hard-surface with a composition comprising cinnamon oil and/or an active thereof |
CN1795925A (en) * | 2004-12-30 | 2006-07-05 | 中国人民武装警察部队医学院 | Medication composition of plant estrogens for treating colpitis |
CN101331876A (en) * | 2006-12-11 | 2008-12-31 | 江苏省农业科学院 | Use of osthole compound thing in preparing pesticide for preventing and curing rice green smut |
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US6753305B2 (en) * | 2000-04-14 | 2004-06-22 | The Procter & Gamble Company | Process for disinfecting a hard-surface with a composition comprising cinnamon oil and/or an active thereof |
CN1795925A (en) * | 2004-12-30 | 2006-07-05 | 中国人民武装警察部队医学院 | Medication composition of plant estrogens for treating colpitis |
CN101331876A (en) * | 2006-12-11 | 2008-12-31 | 江苏省农业科学院 | Use of osthole compound thing in preparing pesticide for preventing and curing rice green smut |
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