CN108294014A - A kind of seed coat agent and the preparation method and application thereof of prevention root rot - Google Patents
A kind of seed coat agent and the preparation method and application thereof of prevention root rot Download PDFInfo
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- CN108294014A CN108294014A CN201810114604.7A CN201810114604A CN108294014A CN 108294014 A CN108294014 A CN 108294014A CN 201810114604 A CN201810114604 A CN 201810114604A CN 108294014 A CN108294014 A CN 108294014A
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- root rot
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- seed coat
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
Abstract
The invention discloses a kind of seed coat agents and the preparation method and application thereof of prevention root rot, are related to technical field of pesticide.The seed coat agent of the prevention root rot of the present invention includes that mass ratio is 15:1:8 20 uniconazole P, Tebuconazole, vicious spirit.The medicament of three kinds of uniconazole P, Tebuconazole and hymexazol different role mechanism is compounded in the present invention, screening obtains optimum compound proportion example, and the compound drug under the ratio is to causing three kinds of pathogenic Fusariumsps of root rot to all have synergistic effect.Further, based on the ratio, the suspension seed-coating agent that suitable auxiliary material is prepared is added, particularly, the suspension seed-coating agent is notable to root rot preventive effect, the early prevention to root rot is realized, has the characteristics that high-efficiency low-toxicity, lasting effect are long, of low cost, easy to use, environmental-friendly, while there is facilitation to the germination of seed.
Description
Technical field
The present invention relates to technical field of pesticide, especially a kind of seed coat agent of prevention root rot and preparation method thereof with answer
With.
Background technology
Root rot is the important silborne fungal diseases in production estimation, and the incidence of the disease is high.Each soybean production in China
Area's root rot generally occurs, and has the characteristics that morbidity is serious, prevention is difficult.Research report both at home and abroad, Fusariumsp is soybean root rot
The main pathogenic bacteria of disease, especially based on Fusarium oxysporum Fusarium oxysporum and fusarium solani F.solani, in addition,
Other a variety of Fusariumsps such as Fusarium graminearum F.graminearum, scouring rush's Fusariumsp F.equiseti, layer give birth to Fusariumsp
F.proliferatum etc. can also cause root rot.The study found that the pathogenic Fusariumsp group of different regions is by cultivation side
The influences such as formula, ecological environment and weather conditions are commonly present difference.Because the host range of Fusariumsp is wide, wheat, corn, big can be endangered
The various crops such as beans, tobacco, and field invalid body, soil surface or it is internal can long-term surviving, caused by leading to Fusariumsp
Root rot once occurs, it is difficult to prevent.
Seed pelleting is to prevent one of the most effective measure of root rot, but produce upper common seed coat agent mostly with single dose
For principal mode, often restricted when in use by factors such as control effect, use scope and poisoning, and with pest and disease damage drug resistance
Enhancing, control effect reduce.Chemical compounding seed coat agent has the characteristics that at low cost, efficient, drug effect is long, easy to use, and energy
Enough generations effectively delayed or avoid pathogen drug resistance.Weems et al. (2015) be respectively adopted fludioxonil, Metalaxyl-M,
The compounded combination of the difference chemical agent such as Fluoxastrobin, metalaxyl, thiophanate methyl handles seed, can to a certain extent effectively
Control soybean sudden death syndrome (SDS).Zhang Rongfang etc. (2015) is dressed seed using the soybean Ultramicro-powder seed coat agent of good fortune gram more than 35%
Processing, to the preventive effect of root rot up to 76.9% or more.Liu Xiulin etc. (2017) utilizes the bright shield of common seed coat agent in the market
(active ingredient is smart first fludioxonil suspension seed-coating agent) and the processing of sharp victory (70% Diacloden seed treatment dispersible pulvis) are big
After beans seed, emergence rate, single-strain fresh weight, plant height and yield are significantly increased.Xing Baolong et al. uses 36.8% Ah mores' good fortune
(avermectin carbendazim thiram) SC, 18% good fortune gram SC, 40% defend good fortune SC prevention root rots, and effect is preferable.
Currently, the type of the upper available root rot seed coat agent of production is still less, it is necessary to develop further types of kind
Clothing agent, to reduce cost, improve drug effect, the popularization and application for being seed coat agent in Soybean production provide technical support.
Invention content
The first invention purpose of the present invention is：In view of the above problems, a kind of kind clothing of prevention root rot is provided
Agent, the seed coat agent by uniconazole P, Tebuconazole and hymexazol compounded combination prepare, have it is efficient, less toxic, at low cost, easy to use,
The features such as environmental-friendly；Apparent to the control effect of root rot, the lasting period is long, at the same can effectively facilitate seed germination and
Strong sprout.
The second goal of the invention of the present invention is to provide a kind of preparation method of the seed coat agent of prevention root rot.
The third goal of the invention of the present invention is to provide the seed coat agent of above-mentioned prevention root rot in prevention root rot and rush
Into the application in germination.
The technical solution adopted by the present invention is as follows：
A kind of seed coat agent of prevention root rot comprising mass ratio 1-5:1:It is the uniconazole P of 8-20, Tebuconazole, vicious
Spirit.
Uniconazole P (uniconazole) is a kind of triazole type plant growth retardent, has efficient, low toxicity, low-residual, drop
The features such as fast is solved, it is short strong, resistant to lodging, anti-aging etc. with positive effect to promoting plant, while having both good bactericidal effect.
When crop copes with adverse circumstance such as arid, high temperature, low temperature, damage or crop failure caused by waterlogging and disease stress, uniconazole P can significantly increase the resistance of crop
Ability has been applied to the plants such as soybean, wheat, rice, rye grass, corn, rape, tomato, cucumber at present, anti-to improve it
Inverse property.In addition, uniconazole P has good control effect to rice seedling blight, wheat scab.Tebuconazole
(tebuconazole) belong to triazole bactericidal agent, have the characteristics that efficient, low toxicity, low-residual, lasting effect is strong, absorbability is strong, be
The seed treatment of Important Economic crop or the efficient germicide of foliage spray, have been used for barley, wheat smut, banded sclerotial blight and kind
Pass the prevention of zonate spot.Hymexazol is a kind of excellent soil sterilants, can efficiently inhibit pythium spp in soil, sickle-like bacteria
Spore germination, and the influence to beneficial bacteria, actinomyces is smaller.But three is individually used to prevent root rot, effect
It is unsatisfactory.
By adopting the above-described technical solution, three is compounded according to the above ratio, to causing three kinds of germs of root rot
Virulence have significantly be added even synergistic effect, can be effectively reduced root rot incidence or prevention root rot.
A kind of seed coat agent of prevention root rot of the present invention comprising mass ratio 3:1:16 uniconazole P, Tebuconazole, evil
Malicious spirit.
By adopting the above-described technical solution, uniconazole P, Tebuconazole, vicious spirit reach optimum proportioning, to three kinds of Fusariumsps
Synergistic effect is all had, it is best to the control effect of root rot.
The seed coat agent of a kind of seed coat agent of prevention root rot of the present invention, the prevention root rot is suspension seed-coating agent.
A kind of seed coat agent of prevention root rot of the present invention, the suspension seed-coating agent further include wetting agent, wetting dispersing agent,
Film forming agent is thickener, antifoaming agent, antifreezing agent, filler, pigment and water.
A kind of seed coat agent of prevention root rot of the present invention, wetting agent is lauryl sodium sulfate, and wetting dispersing agent is benzene
Ethyl phenol polyethenoxy ether, film forming agent are polyvinyl alcohol, and thickener is xanthans acid, and antifoaming agent is n-octyl alcohol, and antifreezing agent is second
Glycol, filler are bentonite, and pigment is rhodamine B.
The seed coat agent of a kind of prevention root rot of the present invention comprising uniconazole P 1-5% based on mass fraction, Tebuconazole
1%, vicious spirit 8-20%, lauryl sodium sulfate 1-3%, phenethyl phenol polyethenoxy ether 3-5%, polyvinyl alcohol 3-5% are yellow
Virgin rubber acid 0.1-0.3%, ethylene glycol 1-3%, n-octyl alcohol 1-3%, rhodamine B 0.4-0.6%, surplus is water.
The seed coat agent of a kind of prevention root rot of the present invention comprising uniconazole P 3% based on mass fraction, Tebuconazole
1%, vicious clever 16%, lauryl sodium sulfate 2%, phenethyl phenol polyethenoxy ether 4%, polyvinyl alcohol 4%, xanthans acid
0.2%, n-octyl alcohol 2%, rhodamine B 0.5%, surplus is water.
By adopting the above-described technical solution, the seed coat agent is easy to use, high-efficiency low-toxicity is of low cost, realizes to root-rot
There is facilitation to germination and strong sprout while the early prevention of sick Fusariumsp.
The preparation method of the seed coat agent of the prevention root rot of the present invention comprising following steps:
Step 1：Film forming agent is taken, distilled water is added, the heating water bath at 60-100 DEG C is brewed into uniform glue
Object；
Step 2：It takes remaining each raw material to be added in film forming agent, is transferred to preliminary grinding 15-25min in colloid mill, is then transferred to more work(
Blender grinding 20-30min can be ground, is finally transferred to high speed emulsification cutter with 7000-10000r/min high-speed stirreds 5-
15min to obtain the final product.
Application of the seed coat agent of prevention root rot provided by the invention in preventing root rot.
Application of the seed coat agent of prevention root rot provided by the invention in promoting germination.
It should be noted that the promotion germination of present invention meaning includes the germination percentage and emergence rate for improving seed.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Three kinds of uniconazole P, Tebuconazole and hymexazol are commonly used but to the very poor pesticide of root rot control effect in the present invention
It is compounded, optimum compound proportion example is sought by experiment, the compound drug under the ratio is equal to three kinds of germs for causing root rot
With synergistic effect.Further, based on the ratio, the suspension seed-coating agent that suitable auxiliary material is prepared is added, realizes
To the early prevention of root rot, have that high-efficiency low-toxicity, duration be long, the spies such as of low cost, easy to use, environmental-friendly
Point, while there is facilitation to the budding of seed.
Specific implementation mode
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated,
It is replaced by other equivalent or with similar purpose alternative features.That is, unless specifically stated, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment 1
The present embodiment provides a kind of seed coat agents of prevention root rot, and it is 1-5 which, which includes mass ratio,:1:8-20's
Uniconazole P, Tebuconazole, vicious spirit.
Embodiment 2
The present embodiment provides a kind of seed coat agent of prevention root rot, which includes that mass ratio is 1-5:1:The alkene of 8-20
Imitate azoles, Tebuconazole, vicious spirit.In addition to this, further include wetting agent, wetting dispersing agent, film forming agent is thickener, antifoaming agent, is prevented
Freeze agent, filler, pigment and water.
Embodiment 3
The present embodiment provides a kind of seed coat agents of prevention root rot, which includes uniconazole P 1- based on mass fraction
5%, Tebuconazole 1%, vicious spirit 8-20%, lauryl sodium sulfate 1-3%, phenethyl phenol polyethenoxy ether 3-5%, polyethylene
Alcohol 3-5%, xanthans acid 0.1-0.3%, ethylene glycol 1-3%, n-octyl alcohol 1-3%, rhodamine B 0.4-0.6%, surplus is water.
The seed coat agent of prevention root rot provided in this embodiment is prepared with the following method：
Step 1：Polyvinyl alcohol is taken in proportion, distilled water is added, and 60-100 DEG C of heating water bath is brewed into uniform, saturating
Jelly bright, without insoluble matter, it is spare.
Step 2：Other each raw materials are weighed in proportion, and surplus is supplied with water, and whole materials are transferred in colloid mill and are carried out just
Then step grinding preliminary grinding 15-25min is transferred to multi-functional grinding blender grinding 20-30min, be finally transferred to high speed emulsification shearing
Machine with 7000-10000r/min high-speed stirreds 5-15min to obtain the final product.
Embodiment 4
The present embodiment provides a kind of seed coat agents of prevention root rot, which includes uniconazole P based on mass fraction
3%, Tebuconazole 1%, vicious clever 16%, lauryl sodium sulfate 2%, phenethyl phenol polyethenoxy ether 4%, polyvinyl alcohol 4%,
Xanthans acid 0.2%, ethylene glycol 2%, n-octyl alcohol 2%, rhodamine B 0.5%, surplus are water.
The seed coat agent of prevention root rot provided in this embodiment is prepared with the following method：
Step 1：Polyvinyl alcohol is taken in proportion, 50% distilled water is added, and 80 DEG C of heating water baths are brewed into uniform, saturating
Jelly bright, without insoluble matter, it is spare.
Step 2：Other each raw materials are weighed in proportion, and surplus is supplied with water, and whole materials are transferred in colloid mill and are carried out just
Then step grinding preliminary grinding 20min is transferred to multi-functional grinding blender grinding 30min, be finally transferred to high speed emulsify cutter with
8000r/min high-speed stirreds 10min to obtain the final product.
Embodiment 5
The present embodiment provides three kinds of Tebuconazole, uniconazole P, hymexazol pair single doses, the uniconazole P and Tebuconazole of different ratio are multiple
Match and the seed coat agent of prevention root rot in embodiment 4 is respectively in vitro Fusarium graminearum, fusarium solani, sharp spore sickle
The Toxicity Determination of spore bacterium, the seed coat agent for the prevention root rot of the present invention are provided fundamental basis.
1. experiment material
96% uniconazole P (uniconzole) active compound (the Shanghai bio tech ltd Yuan Ye), 97% Tebuconazole
(tebuconazole) active compound (Zhengzhou Rui Kai agrochemicals Products Co., Ltd), hymexazol (hymexazol) active compound (Hubei Mao Er
Fertile biological medicine Co., Ltd), the seed coat agent of prevention root rot in embodiment 4；Fusarium oxysporum F.oxyporum, cereal sickle
Spore bacterium F.graminearum and fusarium solani F.solani is isolated from root rot disease sample, through form by this laboratory
And Molecular Identification, 4 DEG C are stored in potato dextrose agar (PDA) solid slope culture medium.
2. experimental method
Using mycelial growth inhibition rate method (NY/T 1156.2-2006, People's Republic of China's agricultural industry criteria [S]
Beijing：Chinese agriculture publishing house, 2006).3 kinds of active compounds are each configured to 5 various concentrations, after filtration sterilization, are added
In the potato agar culture medium (PDA) of sterilizing, and 3% (w/v) streptomysin is added, after mixing well, is prepared into PDA plateEach fusarium bacteria strain for growing 5d on PDA plate is taken, bacteria cake is prepared from colony edge using card punchOne bacteria cake access is added on the PDA plate of different agents, 25 ± 3 DEG C of dark culturing 5d.Each medicament
Each concentration is respectively provided with 3 PDA plates, and connects bacterium tablet as a contrast be not added any medicament, and experiment is repeated 3 times.Respectively
After bacterium grows 5d, colony growth diameter is measured using " crossing method ", different agents is calculated and the mycelia of each Fusariumsp is grown
Inhibiting rate (%).
Calculate medicament virulence, using the logarithm of drug concentration (mg/kg) as independent variable, antibacterial growth rate according to inhibiting rate
NiqueMin is dependent variable, using DPS data processing systems, establishes virulence regression equation y=a+bx, obtains coefficient R2Value,
And calculate half-maximal effect concentration (EC of each medicament to Fusarium graminearum, fusarium solani and Fusarium oxysporum50)。
With the EC of each single dose50, different ratio processing EC50Based on stoichiometric factor, by the virulence for calculating each medicament
Index (TI), the actual toxicity index (ATI) of mixture, the theoretical toxicity index (TTI) of mixture and co-toxicity coefficient (CTC) are to evaluate
Mixture synergy type.It chooses wherein with single dose A medicaments (uniconazole P and any one in Tebuconazole) for standard agent, respectively
Medicament toxicity index calculation formula is as follows：
The theoretical toxicity index (TTI) of mixture=ATI (A) × A%+ATI (B) × B%
Wherein, A%, B% are respectively the percentage composition of two kinds of active compounds in mixture, and ATI (A), ATI (B) are respectively two kinds of originals
The toxicity index of medicine.
Wherein, CTC<80 be antagonism, 80≤CTC<120 be summation action, and 120≤CTC is synergistic effect.
3. experimental result
Three kinds of Tebuconazole, uniconazole P, hymexazol single doses are respectively to Fusarium graminearum, fusarium solani and Fusarium oxysporum
The results are shown in Table 1 for toxicity test.
The Toxicity Determination result of the different single doses of table 1 to Fusariumsp
As shown in Table 1, the pathogenic Fusariumsp of three kinds of Tebuconazole, uniconazole P, hymexazol pair root rots all has inhibition and makees
With, wherein EC of the Tebuconazole to Fusarium graminearum, fusarium solani and Fusarium oxysporum50The μ of respectively 0.78,0.82 and 0.32
G/mL, general performance are best.Uniconazole P is best to the fungistatic effect of Fusarium graminearum, EC50For 0.49 μ g/mL, but to Beancurd sheet sickle
The effect of spore bacterium and Fusarium oxysporum is not so good as Tebuconazole.Hymexazol to the fungistatic effect of Fusarium oxysporum and Fusarium graminearum compared with
It is good, but to the EC of fusarium solani50For 22.12 μ g/mL.
The uniconazole P of different proportion is with Tebuconazole compound drug respectively to Fusarium graminearum, fusarium solani and fusarium oxysporum
The results are shown in Table 2 for the toxicity test of bacterium.
The uniconazole P of 2 different proportion of table compounds the The Toxicity Determination result to Fusariumsp with Tebuconazole
Remarks：Uniconazole P compounds by volume with Tebuconazole, and mother liquid concentration is 12.5 μ g/mL, and the mother liquor after compounding is successively
It is diluted to five concentration gradients, respectively (1/2) of mother liquorn.Wherein, CTC<80, show as antagonism；80≤CTC<120, performance
For summation action；120≤CTC shows as synergistic effect.
As shown in Table 2, uniconazole P and Tebuconazole are 1:3 and 1:When 5,80 are below to the co-toxicity coefficient of Fusarium graminearum,
Antagonism is shown as, but addition or synergy are shown as to other two kinds of Fusariumsps.When the compound proportion of uniconazole P and Tebuconazole is 1:1
With 3:1 and 5:It is to show certain addition or synergistic effect to three kinds of Fusariumsps when 1, but different compound proportion synergy
Coefficient difference.Wherein, with 3:1 effect is best, and the co-toxicity coefficient to three kinds of Fusariumsps is respectively 81.97,137.93 and
833.33 general performance is best.
Further, with uniconazole P and Tebuconazole 3:Based on 1, determines uniconazole P, Tebuconazole and hymexazol difference and match
The virulence of three kinds of Fusariumsps is compared, the results are shown in Table 3.
3 uniconazole P of table, Tebuconazole and hymexazol different volumes are than compounding the The Toxicity Determination result to Fusariumsp
Remarks：Single dose mother liquid concentration is respectively (12.5,12.5,200 μ g/mL), and the mother liquor after compounding is diluted to five successively
A concentration gradient, respectively (1/2) of mother liquorn.Wherein, CTC<70, show as antagonism；70≤CTC<120, it shows as being added and make
With；120≤CTC<170, show as synergistic effect；170≤CTC shows as notable synergistic.
As shown in table 3,120 are much larger than with the co-toxicity coefficient for comparing Fusarium oxysporum for five kinds, show as synergistic effect；When
The proportioning of uniconazole P, Tebuconazole and hymexazol is 3:1:Antagonism is shown as to Fusarium graminearum when 4, other four kinds with analogy
Formula is then synergistic effect；Different ratio has differences the virulence when fusarium solani, and co-toxicity coefficient is different, wherein matching 3:
1:16、3:1:Synergistic effect is shown as to fusarium solani when 20, with 3:1:16 is more cost-effective.
Embodiment 6
The present embodiment provides the safety detections of the seed coat agent of the prevention root rot in embodiment 4, are its application security
Experimental basis is provided.
1. experiment material
No. 12 soya seeds of southern beans (reserving seed for planting in this laboratory), the seed coat agent of the prevention root rot in embodiment 4 is (hereinafter referred to as
This seed coat agent), it is 5% uniconazole P suspension seed-coating agent (seven continent Chemical Co., Ltd. of Jiangsu Zhangjiagang, hereinafter referred to as positive control), clear
Water.
2. experimental method
It is that control is seeded in the flower equipped with culture medium after soya seeds are dressed seed according to each processing dosage with clear water processing
It is each to handle 3 basins per 10, basin in basin (diameter 15cm), it is repeated 3 times.Flowerpot after planting is placed in growth cabinet 25 DEG C,
85%RH, 16h optical culture/8h light cultures, it is long respectively at the 3rd day statistics bud ratio and bud, emergence rate was counted in the 7th day, in the
Emergence rate, plant height, the number of blade and root fresh weight are counted at 15 days.
3. experimental result
Processing is coated to the seed of soybean varieties " southern beans 12 " using each seed coat agent, soybean hair is measured in after planting 5d
Bud rate, such as table 4 as it can be seen that 6 kinds of processing of this seed coat agent soybean germination rate difference compared with clear water compares is not notable, this kind of clothing of low dosage
Agent processing can promote soybean germination to a certain extent, and the processing of 5% Tebuconazole suspension seed-coating agent significantly inhibits soybean germination.
After planting 20d, such as table 4 as it can be seen that compared with clear water compares, different amounts this seed coat agent processing can promote soybean to emerge, but difference
Not significantly, compared with the processing of 5% Tebuconazole suspension seed-coating agent, low this seed coat agent of dosage is remarkably improved soybean emergence, high concentration
(8g/kg) then inhibits.By comparing soybean root fresh weight after different disposal and overground part fresh weight, the results showed that, compared with the control, no
With soybean root fresh weight after dosage this seed coat agent processing without obviously increasing, root fresh weight is notable after the processing of 5% Tebuconazole suspension seed-coating agent
It reduces；Meanwhile after the processing of low this seed coat agent of dosage soybean overground part fresh weight compareed with clear water and 5% Tebuconazole suspension seed-coating agent at
Reason is not notable compared to difference, but overground part fresh weight significantly reduces after the processing of high this seed coat agent of dosage.After different disposal
Soybean plant height, the results showed that, compared with the control, plant height can be significantly inhibited after each dosage processing of this seed coat agent, high dosage inhibits
Effect becomes apparent from, and compared with 5% Tebuconazole suspension seed-coating agent, soybean difference in plant height is not notable after the processing of this seed coat agent.20d
When, each seed coat agent emerge to soya seeds and the influence of upgrowth situation the results are shown in Table 4.
In conclusion compared with clear water is compareed with 5% Tebuconazole suspension seed-coating agent on the market, the low dosage of this seed coat agent
(0.2-1.0g/kg) processed soybeans seed influences unobvious to each growth indexes of soybean, and can promote to a certain extent big
Beans germinate and emergence, reduce plant height, safe.
4 20% alkene penta of table dislikes suspension seed-coating agent indoor security test result
Note：Using the ANOVA analyses in SPSS softwares, significance difference analysis, * same column difference small letters are carried out by LSD
Letter indicates that significance of difference level is P>0.05.
Embodiment 7
The present embodiment provides the measure of merit in practical applications of the seed coat agent of the prevention root rot in embodiment 4, are
The application of the seed coat agent provides further experimental basis,
1. experiment material
The seed coat agent (hereinafter referred to as this seed coat agent) of prevention root rot in embodiment 4；5% uniconazole P suspension seed-coating agent
(seven continent Chemical Co., Ltd. of Jiangsu Zhangjiagang, hereinafter referred to as positive control)；Fusarium oxysporum F.oxyporum, Fusarium graminearum
F.graminearum and fusarium solani F.solani is isolated from root rot disease sample by this laboratory, through morphology and
Molecular Identification, 4 DEG C are stored in potato dextrose agar (PDA) solid slope culture medium；No. 12 soya seeds (this reality of southern beans
It tests room to reserve seed for planting).
2. experimental method
Soybean is inoculated with reference to the beautiful sorghum grain inocalation method for waiting (2014), detection seed coat agent is to root rot
Control efficacy.If 4 concentration processing are arranged in table 6 altogether, using clear water as blank control (CK), soya seeds are handled, fully
For sowing after mixing.
It is packed into 250mL conical flasks after sorghum grain is cooked, 121 DEG C, sterilize 30min, is repeated 1 times, and after its cooling, connects
Enter the pathogen bacteria cake of 10 PDA cultures 7d, cultivates 20d under 25 DEG C of dark conditions, wait for that sorghum grain surface covers with mycelium and is used for
Inoculation.It will be packed into the sterilising medium matter of 1/3 volume in the flowerpot of diameter 15cm, is uniformly sprinkled into the jowar that carries disease germs of 2cm thickness thereon
Grain, then will handle sub- 5-7 and is seeded in sorghum grain, then cover one layer of culture substrate, flowerpot is positioned over people in embodiment 5
Work climatic chamber, 25 DEG C, 85%RH, 16h optical culture/8h light cultures.Each 3 repetitions of processing each repeat 3 flowerpots.Yu Bo
20d investigation emergence rates and incidence after kind, and calculate disease index and kind clothing according to root rot disease scale standard (table 5)
Preventive effect of the agent to root rot.
5 root rot grade scale of table
After being inoculated with using sorghum grain inocalation method, detects each dosage seed coat agent and three kinds of pathogenic Fusariumsps of root rot are prevented
Effect is controlled, the results are shown in Table 6.
Preventive effect of 6 seed coat agents of table to potting soybean Fusariumsp root rot
As shown in Table 6, compared with clear water compares, soybean germination rate and clear water are to photograph after the processing of this seed coat agent different amounts
Compare no significant difference；After being inoculated with three kinds of Fusariumsps, soybean incidence and disease index are significantly lower than untreated control, and high
Dosage handles incidence and disease index is below low concentration processing；Compare the preventive effect of three kinds of Fusariumsps of seed coat agent pair, as a result table
Bright, the processing of 0.2-2.0g/kg seed coat agents reaches 67.35%-80.29% to the preventive effect of Fusarium graminearum, prevents Fusarium oxysporum
Effect reaches 80.06%-89.72%, reaches 70.46%-84.84% to the preventive effect of fusarium solani, it is seen that this seed coat agent is to sharp spore sickle
The preventive effect of spore bacterium is more preferable than other two kinds of Fusariumsps.In conclusion compared with clear water compares, the low dosage (0.2- of this seed coat agent
2.0g/kg) root rot control efficacy caused by three kinds of Fusariumsps of processed soybeans seed pair is preferable, up to 67.35% with
On, dosage more high preventive effect is better, it is contemplated that and the high dosage of this seed coat agent has inhibiting effect (being shown in Table 5) to soybean growth, therefore, it is recommended that
Seed coat agent dosage is 0.2-1.0g/kg.
It should be noted that using soya seeds as experimental subjects in the embodiment of the present invention, but the kind of the prevention root rot
Clothing agent is substantially directed to cause the pathogenic bacteria of root rot, so those skilled in the art are understanding technical solution of the present invention
On the basis of, this seed coat agent can equally be applied to the seed treatment of the various crops such as wheat, corn, paddy, sorghum.
The invention is not limited in specific implementation modes above-mentioned.The present invention, which expands to, any in the present specification to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (10)
1. a kind of seed coat agent of prevention root rot, which is characterized in that it includes that mass ratio is 1-5:1:The uniconazole P of 8-20, penta azoles
Alcohol, vicious spirit.
2. the seed coat agent of prevention root rot according to claim 1, which is characterized in that it includes that mass ratio is 3:1:16
Uniconazole P, Tebuconazole, vicious spirit.
3. the seed coat agent of prevention root rot according to claim 1 or 2, which is characterized in that the kind of the prevention root rot
Clothing agent is suspension seed-coating agent.
4. the seed coat agent of prevention root rot according to claim 3, which is characterized in that the suspension seed-coating agent further includes profit
Humectant, wetting dispersing agent, film forming agent are thickener, antifoaming agent, antifreezing agent, filler, pigment and water.
5. the seed coat agent of prevention root rot according to claim 4, which is characterized in that wetting agent is dodecyl sulphate
Sodium, wetting dispersing agent are phenethyl phenol polyethenoxy ether, and film forming agent is polyvinyl alcohol, and thickener is xanthans acid, and antifoaming agent is
N-octyl alcohol, antifreezing agent are ethylene glycol, and filler is bentonite, and pigment is rhodamine B.
6. the seed coat agent of prevention root rot according to claim 5, which is characterized in that it includes alkene based on mass fraction
Imitate azoles 1-5%, Tebuconazole 1%, vicious spirit 8-20%, lauryl sodium sulfate 1-3%, phenethyl phenol polyethenoxy ether 3-5%, poly- second
Enol 3-5%, xanthans acid 0.1-0.3%, ethylene glycol 1-3%, n-octyl alcohol 1-3%, rhodamine B 0.4-0.6%, surplus is water.
7. the seed coat agent of prevention root rot according to claim 6, which is characterized in that it includes alkene based on mass fraction
Effect azoles 3%, Tebuconazole 1%, vicious clever 16%, lauryl sodium sulfate 2%, phenethyl phenol polyethenoxy ether 4%, polyvinyl alcohol 4%, Huang
Virgin rubber acid 0.2%, n-octyl alcohol 2%, rhodamine B 0.5%, surplus are water.
8. the preparation method of the seed coat agent of the prevention root rot described in any one of claim 4-7, which is characterized in that it includes
Following steps:
Step 1：Film forming agent is taken, distilled water is added, the heating water bath at 60-100 DEG C is brewed into uniform jelly；
Step 2：It takes remaining each raw material to be added in film forming agent, is transferred to preliminary grinding 15-25min in colloid mill, is then transferred to multi-functional grind
Grind blender grind 20-30min, be finally transferred to high speed emulsification cutter with 7000-10000r/min high-speed stirreds 5-15min i.e.
.
9. application of the seed coat agent of the prevention root rot described in any one of claim 1-7 in preventing root rot.
10. application of the seed coat agent of the prevention root rot described in any one of claim 1-7 in promoting germination.
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| CN201810114604.7A CN108294014B (en) | 2018-02-05 | 2018-02-05 | Seed coating agent for preventing and treating root rot and preparation method and application thereof |
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| CN108294014B CN108294014B (en) | 2020-08-04 |
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| CN110692460A (en) * | 2019-10-18 | 2020-01-17 | 中国科学院亚热带农业生态研究所 | Method for planting energy sorghum in cadmium-polluted rice field |
| CN115637241A (en) * | 2022-10-11 | 2023-01-24 | 四川农业大学 | Soybean root rot disease-preventing growth-promoting bacterium RH _ Pc03 and application thereof |
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| CN115637241B (en) * | 2022-10-11 | 2023-09-12 | 四川农业大学 | Soybean root rot disease-preventing growth-promoting bacterium RH_Pc03 and application thereof |
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| CN108294014B (en) | 2020-08-04 |
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