CN104094939B - A kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole - Google Patents
A kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole Download PDFInfo
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Abstract
A kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, belong to technical field of compounded pesticide.It contains copper 8-hydroxyquinolinate and Difenoconazole, and described copper 8-hydroxyquinolinate and the weight ratio of Difenoconazole are 1:10～10:1.Above-mentioned a kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, rationally compounded by copper 8-hydroxyquinolinate and Difenoconazole, copper 8-hydroxyquinolinate and Difenoconazole is made to produce complementary action, copper 8-hydroxyquinolinate is made effectively to make up Difenoconazole deficiency of preventive effect on the lower fungus such as phytophthora, pythium spp, there is notable potentiation, expand prevention and treatment range, improve drug effect.
Description
Technical field
The invention belongs to technical field of compounded pesticide, be specifically related to a kind of containing copper 8-hydroxyquinolinate with the compounding agriculture of Difenoconazole
Medicine.
Background technology
Copper 8-hydroxyquinolinate (copper quinolinate；Copper 8-quinolinolate), chemical name is double (8-hydroxyquinoline base) copper, structural formula
For:
。
It is a kind of organic chelated copper fungicide, and manufacturer is commonly used for seed treatment, is used for preventing and treating various seed-borne disease,
Phytophthora, pythium spp, Alternaria, Botrytis, Phoma fungus there is certain effect, normal and other insecticidal bactericides
Mixed.
Difenoconazole (Difenoconazole；Oppose the pellet that withers), chemical name is suitable, the chloro-4-of trans-3-[4-methyl-2-1H-1,2,4-
Triazol-1-yl methyl)-1,3-bis-mute pentane-2-base) phenyl 4-chlorphenyl ether, structural formula is:
。
It is the triazole bactericidal agent of a kind of high-efficiency broad spectrum, has protection and therapeutical effect concurrently.To ascomycetes, basidiomycetes bacterium
Class, multiple Fungi Imperfecti and how all biography pathogenic fungi have lasting protection and therapeutical effect, and interior absorption is good to crop safety,
It is suitable for use as seed treatment.
It is used alone copper 8-hydroxyquinolinate and Difenoconazole exists preventive effect on the lower fungus such as phytophthora, pythium spp not enough, anti-
Control scope little, and the shortcoming such as drug effect is low.
Summary of the invention
The problem existed for prior art, it is an object of the invention to design provides one containing copper 8-hydroxyquinolinate and phenyl ether methyl cyclic
The technical scheme of the Recompounded pesticide of azoles.
Described a kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, it is characterised in that containing copper 8-hydroxyquinolinate and phenylate first
Ring azoles, described copper 8-hydroxyquinolinate and the weight ratio of Difenoconazole are 1:10～10:1.
Described a kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, it is characterised in that described copper 8-hydroxyquinolinate and phenylate
The weight ratio of methyl cyclic-azole is 1:9～9:1.
Described a kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, it is characterised in that described copper 8-hydroxyquinolinate and phenylate
The weight ratio of methyl cyclic-azole is 1:4～4:1.
Described a kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, it is characterised in that described copper 8-hydroxyquinolinate and phenylate
The weight ratio of methyl cyclic-azole is 3:7～7:3.
Described a kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, it is characterised in that described copper 8-hydroxyquinolinate and phenylate
The weight ratio of methyl cyclic-azole is 2:3～3:2.
Above-mentioned a kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole, rationally multiple by copper 8-hydroxyquinolinate and Difenoconazole
Join, make copper 8-hydroxyquinolinate and Difenoconazole produce complementary action, make copper 8-hydroxyquinolinate effectively make up Difenoconazole at phytophthora, pythium spp
Deng the deficiency of preventive effect on lower fungus, there is notable potentiation, expand prevention and treatment range, improve drug effect.
Detailed description of the invention
The present invention is further illustrated below in conjunction with embodiment.
Embodiment 1 copper 8-hydroxyquinolinate and Difenoconazole Recompounded pesticide, wherein the proportioning of copper 8-hydroxyquinolinate and Difenoconazole is 1:10
～10:1.
The total active constituent content of embodiment 2 medicament 50%, the active constituent content of copper 8-hydroxyquinolinate is 45%, Difenoconazole
Active constituent content is 5%.
The total active constituent content of embodiment 3 medicament 50%, the active constituent content of copper 8-hydroxyquinolinate is 40%, having of Difenoconazole
Effective component content is 10%.
The total active constituent content of embodiment 4 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 35%, Difenoconazole
Active constituent content be 15%.
The total active constituent content of embodiment 5 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 30%, Difenoconazole
Active constituent content be 20%.
The total active constituent content of embodiment 6 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 25%, Difenoconazole
Active constituent content be 25%.
The total active constituent content of embodiment 7 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 20%, Difenoconazole
Active constituent content be 30%.
The total active constituent content of embodiment 8 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 15%, Difenoconazole
Active constituent content be 35%.
The total active constituent content of embodiment 9 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 10%, Difenoconazole
Active constituent content be 40%.
The total active constituent content of embodiment 10 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 5%, Difenoconazole
Active constituent content be 45%.
The total active constituent content of embodiment 11 medicament 50%, the active constituent content of copper 8-hydroxyquinolinate is 45%, Difenoconazole manganese
The active constituent content of salt is 5%.
The total active constituent content of embodiment 12 medicament 50%, the active constituent content of copper 8-hydroxyquinolinate is 40%, Difenoconazole manganese
The active constituent content of salt is 10%.
The total active constituent content of embodiment 13 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 35%, Difenoconazole
The active constituent content of manganese salt is 15%.
The total active constituent content of embodiment 14 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 30%, Difenoconazole
The active constituent content of manganese salt is 20%.
The total active constituent content of embodiment 15 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 25%, Difenoconazole
The active constituent content of manganese salt is 25%.
The total active constituent content of embodiment 16 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 20%, Difenoconazole
The active constituent content of manganese salt is 30%.
The total active constituent content of embodiment 17 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 15%, Difenoconazole
The active constituent content of manganese salt is 35%.
The total active constituent content of embodiment 18 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 10%, Difenoconazole
The active constituent content of manganese salt is 40%.
The total active constituent content of embodiment 19 medicament is 50%, and the active constituent content of copper 8-hydroxyquinolinate is 5%, Difenoconazole
The active constituent content of manganese salt is 45%.
The total active constituent content of embodiment 20 medicament is 20%, and the active constituent content of copper 8-hydroxyquinolinate is 5%, Difenoconazole
The active constituent content of manganese salt is 15%.
The total active constituent content of embodiment 21 medicament is 20%, and the active constituent content of copper 8-hydroxyquinolinate is 8%, Difenoconazole
The active constituent content of manganese salt is 12%.
The total active constituent content of embodiment 22 medicament is 20%, and the active constituent content of copper 8-hydroxyquinolinate is 10%, Difenoconazole
The active constituent content of manganese salt is 10%.
The total active constituent content of embodiment 22 medicament is 20%, and the active constituent content of copper 8-hydroxyquinolinate is 12%, Difenoconazole
The active constituent content of manganese salt is 8%.
The total active constituent content of embodiment 22 medicament is 20%, and the active constituent content of copper 8-hydroxyquinolinate is 15%, Difenoconazole
The active constituent content of manganese salt is 5%.
The total active constituent content of embodiment 23 medicament can be configured to any concentration between 5%～90%, between the two
Proportioning is close or identical with embodiment 1～embodiment 22, but is not limited to above-mentioned concentration.
Embodiment 24 copper 8-hydroxyquinolinate and Difenoconazole compound the seed dressing prevention effect to rice seedling damping-off
1, experiment kind
Dragon round-grained rice 40
2, setting and test method are processed
Process 1 50% quinoline benzene (copper 8-hydroxyquinolinate 15%, Difenoconazole 35%) 1:200 seed dressing
Process 2 50% quinoline benzene (copper 8-hydroxyquinolinate 20%, Difenoconazole 30%) 1:200 seed dressing
Process 3 50% quinoline benzene (copper 8-hydroxyquinolinate 25 %, Difenoconazole 25%) 1:200 seed dressing
Process 4 50% quinoline benzene (copper 8-hydroxyquinolinate 30%, Difenoconazole 20%) 1:200 seed dressing
Process 5 50% quinoline benzene (copper 8-hydroxyquinolinate 40%, Difenoconazole 10%) 1:200 seed dressing
Process 6 75% quinoline benzene (copper 8-hydroxyquinolinate 25%, Difenoconazole 50%) 1:300 seed dressing
Process 7 75% quinoline benzene (copper 8-hydroxyquinolinate 30%, Difenoconazole 45%) 1:300 seed dressing
Process 8 75% quinoline benzene (copper 8-hydroxyquinolinate 40%, Difenoconazole 35%) 1:300 seed dressing
Process 9 75% quinoline benzene (copper 8-hydroxyquinolinate 50%, Difenoconazole 25%) 1:300 seed dressing
Compare 1 50% copper 8-hydroxyquinolinate wettable powder 1:200 seed dressing
Compare 2 10% difenoconazole water dispersible granule 1:40 seed dressings
Compare 3 clear water
Rice paddy seed seed soaking picks up after within 48 hours, showing money or valuables one carries unintentionally, and adds medicament or clear water comparison, then adds proper amount of clear water, and stirring is all
Even, sow after drying.Before rice transplanting, the sickness rate of investigation rice seedling blight, observe the upgrowth situation of rice shoot, neat degree simultaneously
With root system development situation.
And according to the preventive effect of following equation calculating Oryza sativa L. disease:
3, result of the test
Each chemicals treatment and comparison are as shown in table 1 to the prevention effect of rice seedling blight:
Table 1 copper 8-hydroxyquinolinate and Difenoconazole compound the seed dressing prevention effect to rice seedling damping-off
From the point of view of result of the test, in the case of identical active constituent content, each process is used to dress seed to rice seedling blight
Preventive effect between 89.56～97.18%, far above comparison medicament 50% copper 8-hydroxyquinolinate suspending agent 74.28% and 10% phenyl ether methyl cyclic
The 82.67 of azoles water dispersible granules, illustrate that the two is compounding to there being the strongest potentiation in the preventive effect of rice seedling blight.
Each process rice shoot is all neat, and leaf color is dark green, and root system development is good, inconspicuous with contrast difference.
Embodiment 25 copper 8-hydroxyquinolinate and Difenoconazole compound the seed soaking prevention effect to bakanae disease of rice
1, test kind
Golden morning 47
2, setting and test method are processed
Process 4000 times of liquid of 1 40% quinoline benzene (copper 8-hydroxyquinolinate 35%, Difenoconazole 5%)
Process 4000 times of liquid of 2 40% quinoline benzene (copper 8-hydroxyquinolinate 30%, Difenoconazole 10%)
Process 4000 times of liquid of 3 40% quinoline benzene (copper 8-hydroxyquinolinate 25%, Difenoconazole 15%)
Process 4000 times of liquid of 4 40% quinoline benzene (copper 8-hydroxyquinolinate 20%, Difenoconazole 20%)
Process 4000 times of liquid of 5 40% quinoline benzene (copper 8-hydroxyquinolinate 15%, Difenoconazole 25%)
Process 4000 times of liquid of 6 40% quinoline benzene (copper 8-hydroxyquinolinate 10%, Difenoconazole 30%)
Process 4000 times of liquid of 7 40% quinoline benzene (copper 8-hydroxyquinolinate 5%, Difenoconazole 35%)
Process 6000 times of liquid of 8 60% quinoline benzene (copper 8-hydroxyquinolinate 35%, Difenoconazole 25%)
Process 6000 times of liquid of 9 60% quinoline benzene (copper 8-hydroxyquinolinate 25%, Difenoconazole 35%)
Compare 5000 times of liquid of 1 50% copper 8-hydroxyquinolinate wettable powder
Compare 1000 times of liquid of 2 10% difenoconazole water dispersible granule
Compare 3 clear water
Rice paddy seed Medicament soaked seed 48 hours, the long 0.5cm of accelerating germination afterwards to bud, it is seeded on seedling-cultivation plate after somewhat drying.
3 seedling-cultivation plates of each process, often dish 80g seed, use the seedling raising method of dry rice seedling.
3, investigation
When sick seedling length is apparently higher than normal rice shoot, investigate diseased plant number.Calculate sickness rate.
Calculate preventive effect according to the following formula:
4, result of the test:
Each chemicals treatment all shows splendid prevention effect, as shown in table 2.
Table 2 copper 8-hydroxyquinolinate and the compounding prevention effect to bakanae disease of rice of Difenoconazole
| Chemicals treatment | Effective ingredient concentration (PPM) | Preventive effect (%) |
| 1 | 100 | 91.46 |
| 2 | 100 | 95.11 |
| 3 | 100 | 90.93 |
| 4 | 100 | 94.42 |
| 5 | 100 | 89.78 |
| 6 | 100 | 87.60 |
| 7 | 100 | 86.74 |
| 8 | 100 | 91.51 |
| 9 | 100 | 88.60 |
| CK1 | 100 | 72.25 |
| CK2 | 100 | 81.41 |
As can be seen from the test results, each complex treatment all shows splendid prevention effect, preventive effect 86.74～
Between 95.11%, much larger than 72.25% and Difenoconazole single dose of the copper 8-hydroxyquinolinate single dose of equally effective constituent concentration
81.41%, illustrate copper 8-hydroxyquinolinate and Difenoconazole to compound in the preventive effect to bakanae disease of rice and show strong potentiation work
With.
Embodiment 26 copper 8-hydroxyquinolinate and Difenoconazole are compounding to rice green smut and the prevention effect of ear rot
1, test kind
Sense false smut Late-maturing Three-line Japonica Hybrid kind " river in Zhejiang Province excellent 12 "
2, setting and test method are processed
Process 1 50% quinoline benzene (copper 8-hydroxyquinolinate 35%, Difenoconazole 15%) 300 grams/ha
Process 2 50% quinoline benzene (copper 8-hydroxyquinolinate 30%, Difenoconazole 20%) 300 grams/ha
Process 3 50% quinoline benzene (copper 8-hydroxyquinolinate 25%, Difenoconazole 25%) 300 grams/ha
Process 4 50% quinoline benzene (copper 8-hydroxyquinolinate 15%, Difenoconazole 35%) 300 grams/ha
Process 5 50% quinoline benzene (copper 8-hydroxyquinolinate 10%, Difenoconazole 40%) 300 grams/ha
Process 6 50% quinoline benzene (copper 8-hydroxyquinolinate 5%, Difenoconazole 45%) 300 grams/ha
Compare 1 25% copper 8-hydroxyquinolinate suspending agent 600 grams/ha
Compare 2 10% difenoconazole water dispersible granule 1500 grams/ha
Each process is repeated 3 times, and arranges 24 each communities, 30 square metres, every community altogether.
3, medicament using method
It is watered 45,000 grams/acre, Oryza sativa L. cut the last week, sprays.
4, investigation and investigation method
After medicine 35 days, 5 points were selected in every community, and every some investigation 200 fringes, by corresponding grade scale investigation rice green smut and Oryza sativa L.
The incidence of ear rot.
The grade scale of rice green smut is as shown in the table
| Sick level | 0 | 1 | 3 | 5 | 7 | 9 |
| Infected seed number/fringe | 0 | 1 | 2-4 | 5-8 | 9-13 | ≥14 |
| Fringe heavily loses % | 0 | 0.1-5.0 | 5.1-10.0 | 10.1-20.0 | 20.1-50.0 | ≥50.1 |
The grade scale of Rice Panicle maize ear rot is as shown in the table:
| Sick level | Grade scale |
| 0 grade | Without scab |
| 1 grade | Every fringe loss less than 1% |
| 3 grades | Every fringe loss 2%～5% |
| 5 grades | Every fringe loss 6%～15% |
| 7 grades | Every fringe loss 16%～25% |
| 9 grades | Every fringe loss more than 26% |
Computing formula and statistical analysis:
5, result of the test
Copper 8-hydroxyquinolinate and the compounding and each positive control medicament of Difenoconazole are to rice green smut and the preventing and treating of Rice Panicle maize ear rot
As a result, as shown in table 3:
Table 3 copper 8-hydroxyquinolinate and Difenoconazole are compounding to rice green smut and the prevention effect of ear rot
| Chemicals treatment | Effective ingredient consumption (gram/ha) | Preventive effect to rice green smut | Preventive effect to Rice Panicle maize ear rot |
| 1 | 150 | 89.68 | 88.51 |
| 2 | 150 | 86.31 | 90.31 |
| 3 | 150 | 86.73 | 86.54 |
| 4 | 150 | 86.66 | 90.03 |
| 5 | 150 | 88.25 | 86.61 |
| 6 | 150 | 89.45 | 87.35 |
| CK1 | 150 | 76.34 | 74.92 |
| CK2 | 150 | 71.21 | 66.53 |
In the preventive effect to rice green smut, the preventive effect of copper 8-hydroxyquinolinate and each complex treatment of Difenoconazole 86.31～
Between 89.68%, 76.34% and 71.21% processed far above two single doses, illustrate that copper 8-hydroxyquinolinate and Difenoconazole are to Oryza sativa L. rice
Strong potentiation is shown in bent sick preventive effect.In the preventive effect to Rice Panicle maize ear rot, copper 8-hydroxyquinolinate and Difenoconazole
Preventive effect between 86.54%～90.31%, far above the 66.53～74.92% of two single dose control treatment, copper 8-hydroxyquinolinate and benzene are described
Ether methyl cyclic-azole shows potentiation in the preventive effect to Rice Panicle maize ear rot.
Thus illustrating, copper 8-hydroxyquinolinate and Difenoconazole compound in Rice Growing later stage disease this kind of to false smut and ear rot
Prevention effect notable, potentiation is obvious.
Other each formula is to rice green smut and the preventing and treating also table of Rice Panicle maize ear rot this kind of Rice Growing later stage fungal disease
Reveal same potentiation, do not repeated them here.
Embodiment 27 copper 8-hydroxyquinolinate and the compounding prevention effect to Rice blast of Difenoconazole
1, test kind
Super hybridization rice kind " excellent No. one of middle Zhejiang "
2, test processes and arranges
Process 1 20% quinoline benzene (copper 8-hydroxyquinolinate 15%, Difenoconazole 5%) 600 grams/ha
Process 2 20% quinoline benzene (copper 8-hydroxyquinolinate 13%, Difenoconazole 7%) 600 grams/ha
Process 3 20% quinoline benzene (copper 8-hydroxyquinolinate 10%, Difenoconazole 10%) 600 grams/ha
Process 4 20% quinoline benzene (copper 8-hydroxyquinolinate 7%, Difenoconazole 13%) 600 grams/ha
Process 5 20% quinoline benzene (copper 8-hydroxyquinolinate 5%, Difenoconazole 15%) 600 grams/ha
Compare 1 20% copper 8-hydroxyquinolinate suspending agent 600 grams/ha
Compare 2 10% difenoconazole water dispersible granule 1200 grams/ha
3, medicament using method
It is watered 30,000 grams/acre, in Oryza sativa L. cut phase, spraying.
4, investigation method and investigation
The generation disease index of 25 days investigation Rice blasts after dispenser, 35 days investigation paddy bacterial glume blights after dispenser
Morbidity clump sickness rate, by community harvesting statistics yield.
The grade scale of Rice blast is as shown in the table
| Sick level | Panicle blast victimization state | Single fringe victimization state |
| 0 | Disease tassel yield is less than 1% | On fringe anosis |
| 1 | Disease tassel yield 1-5% | Every fringe loss rate≤5%(other sprig stalk morbidity) |
| 3 | Disease tassel yield is 5.1-10% | Branch stalk morbidity about every fringe loss rate 5.1-15%(1/10 ~ 1/5) |
| 5 | Disease tassel yield is 10.1-25% | Branch stalk morbidity about every fringe loss rate 15.1-30%(1/5 ~ 1/3) |
| 7 | Disease tassel yield is 25.1-50% | Every fringe loss rate 30.1-50%(fringe neck or main shaft morbidity, grain half is flat) |
| 9 | Disease tassel yield >=50.1% | Every fringe loss rate >=50.1%(fringe neck morbidity, major part shrivelled kernel or cause dead ears) |
To Rice blast preventive effect computational methods:
5, result of the test
Copper 8-hydroxyquinolinate and each complex treatment of Difenoconazole and positive control are to prevention effect such as table 4 institute to Rice blast
Show:
Table 4 copper 8-hydroxyquinolinate and the compounding prevention effect to Rice blast of Difenoconazole
| Pesticide-treated | Effective ingredient consumption (gram/ha) | Prevention effect (%) |
| 1 | 120 | 86.37 |
| 2 | 120 | 88.60 |
| 3 | 120 | 85.53 |
| 4 | 120 | 88.20 |
| 5 | 120 | 85.55 |
| CK1 | 120 | 63.41 |
| CK2 | 120 | 75.63 |
From the point of view of result of the test, each process the preventive effect to Rice blast between 85.53～88.60%, higher than two single doses
The 63.41% and 75.63% of comparison, illustrating copper 8-hydroxyquinolinate and Difenoconazole to compound Rice blast has preventive effect notable, and both
Compounding potentiation is obvious.
Embodiment 28 copper 8-hydroxyquinolinate and Difenoconazole compounding to rice sheath blight disease, rice blast, wheatear maize ear rot, little
The diseases such as wheat banded sclerotial blight have preferable prevention effect, and potentiation is obvious, repeats the most one by one at this.
Embodiment 29 copper 8-hydroxyquinolinate and the compounding indoor biometrics to fusarium moniliforme of Difenoconazole
1, medicament preparation
The former medicine of copper 8-hydroxyquinolinate is made into suspension through ultrasonic Treatment, through a small amount of acetone solution, utilizes sterilized water to be diluted to respectively
800mg/ml and 400mg/ml mother solution.Arrange according to mixture purpose and organize dosages distribution more, 1:9,1:4,3:7,2:3,1:1,3:2,
7:3,4:1,9:1, each proportioning is the mass ratio of two kinds of medicament effective ingredient.
2, test strain
Fusarium moniliforme.
3, test method
Indoor virulence test to fusarium moniliforme uses mycelia performance rate method to carry out.
Under aseptic technique, according to test process, the sterilising medium melted in advance quantitatively (generally 60ml) is added
Enter in aseptic conical flask, from low concentration to high concentration, the most quantitatively draw medicinal liquid, be separately added in above-mentioned conical flask, fully shake
Even.Then, during equivalent pours the culture dish that 3 diameter above are 9cm into, the pastille flat board of respective concentration is made.Test sets not pastille
Blank is made in the process of agent, and each process is no less than 3 repetitions.
The Fusarium moniliforme Sheld (cell age about 5 days) culture medium supported, aseptically with the sterilizing punching of diameter 5mm
Device, cuts bacterium cake from colony edge, with inoculator, bacterium cake is connected to pastille flat board central authorities, and mycelia faces up, and covers ware lid, puts suitable
Preferably the incubator of temperature is cultivated.
According to the growing state investigation pathogen mycelial growth situation of bacterium in blank culture dish.Use kind of calliper bacterium colony
Diameter, unit is millimeter (mm).Each bacterium colony decussation method vertical survey diameter is each once.
According to survey result, formula 1 and formula 2 is utilized to calculate each concentration for the treatment of to the mycelial growth suppression for examination target bacterium
Rate, unit is percentage rate (%), and result of calculation retains 2 significant digits.
... ... ... ... ... ... ... ... ... formula (1)
In formula:
-bacterium colony increases diameter；
-colony diameter；
-bacterium cake diameter.
... ... ... ... ... ... ... ... formula (2)
In formula:
-mycelial growth inhibition rate；
-blank bacterium colony increases diameter；
-chemicals treatment bacterium colony increases diameter.
Utilize Tang Qi justice DPS data analysis software, according to each drug concentration logarithm value and the mycelial growth inhibition rate of correspondence
Probability value makees regression analysis, calculates each medicament.
4, compounding effect assessment
On the basis of each single dose and built agent EC50 value, calculate each built agent coefficient of synergism (SR) according to Wadley method,
SR value is utilized to evaluate compounding effect.SR >=1.5 represent have potentiation；SR≤0.5 represents have antagonism；0.5<SR<
1.5 is summation action.
Coefficient of synergism (SR) presses formula (6) and formula (7) calculates:
... ... ... ... ... ... ... formula (3)
In formula:
The theoretical value of-mixture, unit is milligrams per liter (mg/L)；
The percentage composition of A in-mixture, unit is percentage rate (%)；
The percentage composition of B in-mixture, unit is percentage rate (%)；
The theoretical value of A in-mixture, unit is milligrams per liter (mg/L)；
The theoretical value of B in-mixture, unit is milligrams per liter (mg/L).
... ... ... ... ... ... ... ... ... ... ... formula (4)
In formula:
The coefficient of synergism of-mixture；
-mixture theoretical value, unit is milligrams per liter (mg/L)；
-mixture measured value, unit is milligrams per liter (mg/L).
5, result of the test
Copper 8-hydroxyquinolinate and Difenoconazole are compounding as shown in the table to the indoor biometrics result of fusarium moniliforme:
| Reagent agent | Actual EC50(ppm) | Theoretical EC50(ppm) | Coefficient of synergism |
| Copper 8-hydroxyquinolinate | 5.35 | / | / |
| Difenoconazole | 0.096 | / | / |
| Quinoline benzene proportioning 1:9 | 0.034 | 0.106 | 3.09 |
| Quinoline benzene proportioning 1:4 | 0.037 | 0.119 | 3.24 |
| Quinoline benzene proportioning 3:7 | 0.030 | 0.136 | 4.50 |
| Quinoline benzene proportioning 2:3 | 0.029 | 0.158 | 5.40 |
| Quinoline benzene proportioning 1:1 | 0.027 | 0.189 | 7.11 |
| Quinoline benzene proportioning 3:2 | 0.038 | 0.234 | 6.07 |
| Quinoline benzene proportioning 7:3 | 0.050 | 0.307 | 6.11 |
| Quinoline benzene proportioning 4:1 | 0.084 | 0.448 | 5.34 |
| Quinoline benzene proportioning 9:1 | 0.133 | 0.826 | 6.23 |
From the point of view of result of the test, Difenoconazole on the suppression efficiency of fusarium moniliforme is greater than copper 8-hydroxyquinolinate, but
The two compounding coefficient of synergism, between 3.09 to 7.11, much larger than 1.5, illustrates that Difenoconazole and oxine-copper composite show
Strong potentiation.
Embodiment 30 Difenoconazole and the oxine-copper composite indoor biometrics to rice blast fungus
1, medicament preparation
With embodiment 29
2, test strain
Pathogen of Rice Blast Fungus bacterial strain: Magnaporthe grisea Pyriculariaoryae WZ30, this bacterial strain belongs to the strong of ZB population
Pathogenic strain.
3, test method
By cultured rice blast fungus spore deionized water eluting, filtration from culture medium or diseased tissues, centrifugal
(1000r/min) 5min, goes the stillness of night, adds deionized water, centrifugal.Finally with deionized water by resuspended for spore floating to every milli
Rise 1 × 105～1 × 107Individual spore, and add 0.5% glucose solution.
With pipet or pipettor from low concentration to high concentration, draw medicinal liquid 0.5mL successively and be separately added in small test tube, so
The spore suspension 0.5mL that rear absorption prepares, makes medicinal liquid uniform with spore suspension mixed in equal amounts.Draw with micro sample adding appliance
Above-mentioned admixing medical solutions is on concave slide, and then frame is put in the crisper with shallow seated groundwater, adds a cover moisturizing and is incubated at preference temperature
Incubator in.Often process and repeat no less than 3 times, and set the process without medicament and make blank.
When blank spore germination rate reaches more than 90%, check each process spore germination situation
When processing more than 3 visuals field of each repetition random observation, investigation spore sum, no less than 200, records sprouting respectively
Number and spore sum, spore germ tube length is considered as sprouting more than the short radius of spore.
According to survey data, calculating the spore germination relative inhibition of each process, unit is percentage rate (%).By formula
(5), (6) and (7) calculate, result of calculation retain 2 significant digits:
... ... ... ... ... ... ... ... ... formula (5)
In formula:
-spore germination rate；
-spore germination number
The spore sum of-investigation
... ... ... ... ... ... ... ... ... formula (6)
In formula
-process correction spore germination rate
-process spore germination rate
-blank spore germination rate
... ... ... ... ... ... ... ... ... formula (7)
In formula
-spore germination relative inhibition；
-blank spore germination rate；
-process the spore germination rate corrected.
Probability value according to each drug concentration logarithm value and the spore germination relative inhibition of correspondence makees regression analysis, calculates
Each medicament.
4, compounding effect assessment
With embodiment 29.
5, result of the test
Difenoconazole and the oxine-copper composite Toxicity Determination result to rice blast fungus, as shown in the table:
| Reagent agent | Actual EC50(ppm) | Theoretical EC50(ppm) | Coefficient of synergism |
| Copper 8-hydroxyquinolinate | 10.07 | / | / |
| Difenoconazole | 0.125 | / | / |
| Quinoline benzene proportioning 1:9 | 0.089 | 0.139 | 1.55 |
| Quinoline benzene proportioning 1:4 | 0.045 | 0.156 | 3.49 |
| Quinoline benzene proportioning 3:7 | 0.069 | 0.178 | 2.57 |
| Quinoline benzene proportioning 2:3 | 0.049 | 0.207 | 4.2 |
| Quinoline benzene proportioning 1:1 | 0.067 | 0.247 | 3.69 |
| Quinoline benzene proportioning 3:2 | 0.242 | 0.307 | 1.27 |
| Quinoline benzene proportioning 7:3 | 0.182 | 0.405 | 2.23 |
| Quinoline benzene proportioning 4:1 | 0.213 | 0.595 | 2.79 |
| Quinoline benzene proportioning 9:1 | 0.450 | 1.124 | 2.5 |
From the point of view of result of the test, Difenoconazole on the suppression efficiency of rice blast fungus is greater than copper 8-hydroxyquinolinate, but
The two compounding coefficient of synergism except quinoline benzene proportioning 3:2 be 1.27 show as summation action in addition to, other each formula is both greater than 1.5, says
Bright Difenoconazole and oxine-copper composite show strong potentiation in the suppression to fusarium moniliforme.
Embodiment 31 Difenoconazole and the oxine-copper composite indoor biometrics to Ustilaginoidea virens
1, medicament preparation
With embodiment 29
2, test strain
Rice green smut bacteria strain Ustilaginoideavirens UV-13
3, test method
With embodiment 29
4, compounding effect assessment
Example 29 simultaneously
5, result of the test
Difenoconazole and copper 8-hydroxyquinolinate are to Ustilaginoidea virens indoor bioassay result, as shown in the table:
From the point of view of result of the test, Difenoconazole and the copper 8-hydroxyquinolinate EC to rice green smut50It is respectively 0.031 and 3.91,
Show relatively high inhibition effect.Both compound synergic coefficients are between 3.71～6.58, and potentiation is obvious.
Claims (2)
1. one kind contains copper 8-hydroxyquinolinate and the Recompounded pesticide of Difenoconazole, it is characterised in that containing copper 8-hydroxyquinolinate and Difenoconazole, institute
The copper 8-hydroxyquinolinate stated and the weight ratio of Difenoconazole are 1:9～2:3.
2. one kind contains copper 8-hydroxyquinolinate and the Recompounded pesticide of Difenoconazole, it is characterised in that containing copper 8-hydroxyquinolinate and Difenoconazole, institute
The copper 8-hydroxyquinolinate stated and the weight ratio of Difenoconazole are 3:2～9:1.
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| WO2000027200A1 (en) * | 1998-11-06 | 2000-05-18 | Syngenta Participations Ag | FUNGICIDAL COMBINATIONS COMPRISING THIENO[2,3-d]PYRIMIDIN-4-ONE |
| CN101107932A (en) * | 2007-07-11 | 2008-01-23 | 湖南万家丰科技有限公司 | Bactericial composition containing Difenoconazole and copper fungicide |
| CN102308806A (en) * | 2011-04-28 | 2012-01-11 | 广东中迅农科股份有限公司 | Pesticide composition containing oxine-copper and epoxiconazole |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2000027200A1 (en) * | 1998-11-06 | 2000-05-18 | Syngenta Participations Ag | FUNGICIDAL COMBINATIONS COMPRISING THIENO[2,3-d]PYRIMIDIN-4-ONE |
| CN101107932A (en) * | 2007-07-11 | 2008-01-23 | 湖南万家丰科技有限公司 | Bactericial composition containing Difenoconazole and copper fungicide |
| CN102308806A (en) * | 2011-04-28 | 2012-01-11 | 广东中迅农科股份有限公司 | Pesticide composition containing oxine-copper and epoxiconazole |
Non-Patent Citations (1)
| Title |
|---|
| 药剂拌种对大麦条纹病的防治效果;杜文芳等;《植物保护》;20130608;第39卷(第3期);第190-192页，参见第192页右栏 * |
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