CN109221234A - A kind of composition and its application method with bactericidal activity - Google Patents
A kind of composition and its application method with bactericidal activity Download PDFInfo
- Publication number
- CN109221234A CN109221234A CN201811246726.8A CN201811246726A CN109221234A CN 109221234 A CN109221234 A CN 109221234A CN 201811246726 A CN201811246726 A CN 201811246726A CN 109221234 A CN109221234 A CN 109221234A
- Authority
- CN
- China
- Prior art keywords
- composition
- seed
- agent
- bactericidal activity
- disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
- A01N55/02—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of compositions and its application method with bactericidal activity for belonging to pesticide range；The composition with bactericidal activity is to organize compound and at least one (b) by least one (a) to organize the mixture that compound forms；Its (a) organizes the chelate Mn (8-HQ) that compound is manganese metal and 8-hydroxyquinoline (8-HQ) shown in general formula A, and (b) organizing compound is metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole etc.；It is mixed object and is processed into sprayable preparation；And using atomizers spray on the cauline leaf of plant, to prevent and treat the disease of aboveground vegetation part, or it is processed into seed clothing agent for seed pelleting, to promote seed quickly to emerge and prevent and treat the disease of foot end.Toxicity test is the results show that the coefficient of synergism of composition is all larger than 1.5；To rice, wheat, the disease control ability of vegetables and fruit is had obvious synergistic function, and is better than single dose kind.
Description
Technical field
The invention belongs to pesticide range, in particular to a kind of composition and its application method with bactericidal activity.
Background technique
Crop disease is varied, and producing region distribution is different, and amblent air temperature is different, and disease species are also all kinds of.In crop
The use of fungicide is the important measures for controlling disease and occurring during disease control, provides effective guarantor for stable crop yield high yield
Barrier.But fungicide use often exist control efficiency it is undesirable and there are disease to fungicide generate resistance cause prevention and treatment imitate
The problems such as fruit declines.For example, metalaxyl was once the control very effective fungicide of crop oomycetes disease, but lasting use for many years
Cause Metalaxyl sensitivity development serious, metalaxyl is used alone now it is difficult to ensure that ideal control efficiency.In practical agriculture
In industry production, it is to improve bactericidal agent for preventing and treating effect and delay germ to sterilization that the fungicide with synergistic function, which is used in mixed way,
The effective means of agent resistance.
Summary of the invention
The object of the present invention is to provide a kind of composition and its application method with bactericidal activity；It is characterized in that, institute
Stating the composition with bactericidal activity is to organize compound and at least one (b) by least one (a) to organize the mixing that compound forms
Object；(a) organizes the chelate that compound is manganese metal and 8-hydroxyquinoline (8-HQ) shown in general formula A, as n=2 in general formula A
For Mn (8-HQ)2Chelate；It is as n=3 in general formula A Mn (8-HQ)3Chelate；
(b) organize compound be metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole, Prochloraz, Fluoxastrobin,
Isoprothiolane, kasugarnycin, fenoxanil, carbendazim, pyrimethanil, diethofencarb, fluazinam, Boscalid, cymoxanil, Tebuconazole,
Epoxiconazole, nitrile bacterium azoles, propiconazole, Cyproconazole, simeconazoles, triticonazole, Flusilazole, hexaconazole, kind bacterium azoles, difenoconazole,
It is a kind of in SSF 126, orysastrobin, fluoxastrobin, kresoxim-methyl, ZEN 90160, pyraclostrobin, trifloxystrobin and fluopicolide
Or it is several.
It is 100:1~1:100 that (a), which organizes compound and the weight ratio of (b) combination of compounds, in the mixture.
The mixture is processed into wettable powder, water dispersible granules, suspending agent and cream preparation；And by any one of them
Using atomizers spray on the cauline leaf of plant, to prevent and treat the disease of aboveground vegetation part, or be processed into seed clothing agent for kind
Attached bag clothing, to promote seed quickly to emerge and prevent and treat the disease of foot end.
(a) organizes compound and is used in mixed way with (b) group compound, has significant synergistic function, is used for plant
Being used alone when disease control than it has superior bactericidal activity.
The composition belongs to broad spectrum activity fungicide, effectively kills to pathogenic oomycetes and fungi.
The application method of the composition with bactericidal activity；It is characterised by comprising:
(1) bactericide compounded indoor toxicity test standard is determined using Plating, i.e., using Plating measurement medicament to disease
The inhibiting effect of opportunistic pathogen mycelia growth；First by (a) group compound and the compound bactericidal agent original medicinal alcohol dissolution of (b) group, prepare
It is spare at the medical fluid of series of concentrations, then medical fluid is added to the PDA culture medium for having melted and being cooled to room temperature in 1% ratio
In, it after sufficiently shaking up, pours into the culture dish (Φ 90mm) of sterilizing and band medicine plate is made, the PDA that room temperature is added in sterile water is trained
It supports in base, cultivates pathogen；By cultured pathogen, under aseptic technique with sterilization punchers (Φ 5mm) selection length
The consistent bacterium colony of gesture, cuts bacteria cake from colony edge, with inoculator by pure culture biscuits involvng inoculation in band medicine plate center, mycelia face mycelia face
Downwards, ware lid is covered, culture in 25 DEG C of constant incubator is placed in, blank control is made with the processing without medicament, after culture 6 days
Pathogen hypha growth condition is investigated, the colony diameter of each processing is measured using crossing method, and utilize regression analysis pair
Test data is analyzed, and calculates each medicament to the EC of pathogen50, the virulence size of more each treatment agent, wherein EC50
It is fungicidal activity evaluation index；Coefficient of synergism (SR) is calculated by formula (1) and formula (2):
(a) group compound synergy type mixed with (b) group compound bactericidal agent is evaluated according to Wadley method, is increased
Effect coefficient (SR)<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect；
(2) by (a) group compound, 100:1~1:100 is mixed according to above-mentioned weight ratio with (b) group compound, is used in combination,
And it is processed into wettable powder, water dispersible granules, suspending agent and cream preparation；Then any one of them is used into atomizers spray
On the cauline leaf of plant, to prevent and treat the disease of aboveground vegetation part, or seed clothing agent is processed into for seed pelleting, to promote to plant
The disease of sub quickly emergence and prevention and treatment foot end；Then the collaboration of two groups of compounds is calculated according to formula (1), formula (2)
Coefficient of synergism,
The prevention and treatment plant includes to rice, wheat, the disease control of fruit and vegetables, fruit.
(a) organizes compound and (b) group compound bactericidal agent is mixed to rice blast pathogen virulence, comprising:
Mn(8-HQ)2It compounds with tricyclazole, Prochloraz, propiconazole, difenoconazole to rice blast pathogen virulence, with Mn
(8-HQ)2It is medicament to be measured using Pyricularia oryzae as subjects with tricyclazole, Prochloraz, propiconazole, difenoconazole, according to upper
It states test method and carries out indoor bioassay, blank control is made with the processing without medicament, in triplicate.Test result is in weight
Amount is than Mn in the range of 5:1-1:5 (8-HQ)2With the synergy system of tricyclazole, Prochloraz, propiconazole or difenoconazole composition
Number is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, control efficiency is excellent and is better than single dose
Kind.
Mn(8-HQ)3It compounds with Fluoxastrobin, Prochloraz etc. to rice blast pathogen virulence, with Mn (8-HQ)3With Fluoxastrobin, miaow
Fresh amine is medicament to be measured, Pyricularia oryzae is subjects, carries out indoor bioassay according to above-mentioned test method, to be free of medicament
Processing make blank control, test result is shown, the Mn (8-HQ) in the range of weight ratio 5:1-1:53It is fresh with tricyclazole or miaow
The coefficient of synergism of amine composition is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, prevention and treatment effect
Fruit is excellent and is better than single dose product.
The Mn (8-HQ)3Cucumber downy mildew spore germination is inhibited with metalaxyl, compounding for cyazofamid, is sprouted using spore
Hair method, respectively by Mn (8-HQ)3, metalaxyl, cyazofamid dissolved with ethyl alcohol, then be watered and be diluted to 0.1,1,10,20,50,100,
The suspension of 200mg/L；Fresh mould layer is taken to prepare spore suspension on the sick leaf of cucumber downy mildew, with institute's dose out powders dilution
Mixing, every processing are repeated 6 times, and are placed in insulating box, and spore germination situation is investigated in 25 DEG C of cultures afterwards for 24 hours, and toxicity test result is aobvious
Show, the Mn (8-HQ) in the range of weight ratio 10:1-1:103It is all larger than with the coefficient of synergism of metalaxyl or cyazofamid composition
1.5, cucumber downy mildew spore germination is had obvious synergistic function, especially at Mn (8-HQ)3With metalaxyl or cyanogen frost
Synergistic effect is most significant when the weight ratio of azoles is 1:5.
The anti-wheat sharp eyespot of seed pelleting, taking 20g weight ratio is the Mn (8-HQ) of 4:13With mixing for difenoconazole
Close object, 8g dispersing agent 2700,5g wetting agent op-10,0.3g xanthan gum, 2g aluminium-magnesium silicate, 4g ethylene glycol, 3g film forming agent NDSF,
It to average particle size is 1~3 micron that 2g rhodamine toner and 55.7g water are levigate in sand mill, and 20% suspension seed pelleting is made
Agent；During field trial, wheat seed is short by anti-58, coating pesticide-seeds ratio: 1:400,1:200,1:100 (weight ratio), small
Wheat application rate is 230kg/ha；After planting January investigates growth of seedling situation, does not find that any symptom of chemical damage, emergence rate are big
In 90%；Therefore, 20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed is safe to wheat；In wheat jointing
Banded sclerotial blight incidence is investigated afterwards, calculates disease index and control efficiency；20%Mn (8-HQ)3Difenoconazole suspending seed
Coating agent is in coating pesticide-seeds ratio: being respectively 60.2%, 80.7% and to sheath blight disease controlling effect under 1:400,1:200,1:100
89.3%.Therefore, 20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed coating wheat seed can effectively prevent wheat
Banded sclerotial blight.
The beneficial effects of the invention are as follows the present invention by mixed with (b) group compound bactericidal agent to (a) group compound, to water
Rice, wheat, the disease virulence test of fruit and vegetables, fruit, toxicity test is the results show that the coefficient of synergism of composition is all larger than
1.5, the prevention and control capability of Pyricularia oryzae is had obvious synergistic function, control efficiency is excellent and is better than single dose product.To Huang
Melon downy mildew spore germination has obvious synergistic function, especially at Mn (8-HQ)3With the weight of metalaxyl or cyazofamid
Synergistic effect is most significant when than for 1:5.20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed is coated wheat seed to line
Blight control efficiency is respectively 60.2%, 80.7% and 89.3%.Therefore, 20%Mn (8-HQ)3Difenoconazole suspending seed
Coating agent coating wheat seed can effectively prevent wheat sharp eyespot；It is safe to the emergence of wheat, growth of seedling.
Specific embodiment
The present invention provides a kind of composition and its application method with bactericidal activity；The combination with bactericidal activity
Object is to organize compound and at least one (b) by least one (a) to organize the mixture that compound forms；(a) organizes compound
The chelate of manganese metal shown in general formula A and 8-hydroxyquinoline (8-HQ) is Mn (8-HQ) as n=2 in general formula A2Chelate；When
It is when n=3 in general formula A Mn (8-HQ)3Chelate；
(b) organize compound be metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole, Prochloraz, Fluoxastrobin,
Isoprothiolane, kasugarnycin, fenoxanil, carbendazim, pyrimethanil, diethofencarb, fluazinam, Boscalid, cymoxanil, Tebuconazole,
Epoxiconazole, nitrile bacterium azoles, propiconazole, Cyproconazole, simeconazoles, triticonazole, Flusilazole, hexaconazole, kind bacterium azoles, difenoconazole,
It is a kind of in SSF 126, orysastrobin, fluoxastrobin, kresoxim-methyl, ZEN 90160, pyraclostrobin, trifloxystrobin and fluopicolide
Or it is several.
In order to better illustrate the present invention, it is further described below by embodiment.
One, embodiment determines bactericide compounded indoor toxicity test standard using Plating,
The test method of indoor bioassay: referring to People's Republic of China's agricultural industry criteria NY/T 1156.2-
2006, the inhibiting effect that pathogen mycelia is grown using Plating measurement medicament.(a) group compound and (b) are first organized into chemical combination
The former medicinal alcohol of object fungicide dissolves, and the medical fluid for being configured to series of concentrations is spare；Then medical fluid is added in 1% ratio
In the PDA culture medium for melting and being cooled to room temperature, after sufficiently shaking up, pours into the culture dish (Φ 90mm) of sterilizing to be made and be put down with medicine
Plate, the processing of sterile water to the PDA culture medium for having melted and being cooled to room temperature is added as blank control, every processing repeats three
It is secondary；By cultured pathogen, the consistent bacterium colony of growing way is selected with sterilization punchers (Φ 5mm) under aseptic technique, from
Colony edge cuts bacteria cake, and with inoculator by pure culture biscuits involvng inoculation in band medicine plate center, mycelia face mycelia downwards, covers ware lid,
It is placed in culture in 25 DEG C of constant incubator, blank control is made with the processing without medicament, in triplicate, culture is investigated after 6 days
Pathogen hypha growth condition measures the colony diameter of each processing using crossing method, and using regression analysis to test
Data are analyzed, and calculate each medicament to the EC50 of pathogen, the virulence size of more each treatment agent, wherein EC50It is to kill
Microbial inoculum activity rating index；Coefficient of synergism (SR) is calculated by formula (1) and formula (2):
(a) group compound synergy type mixed with (b) group compound bactericidal agent is evaluated according to Wadley method, is increased
Effect coefficient (SR)<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect.
Two, the bactericide compounded indoor virulence of bioassay,
Embodiment 1, Mn (8-HQ)2It compounds with tricyclazole, Prochloraz etc. to rice blast pathogen virulence,
With Mn (8-HQ)2With tricyclazole, Prochloraz be medicament to be measured, Pyricularia oryzae is subjects, according to above-mentioned test
Method carries out indoor bioassay, makees blank control with the processing without medicament, in triplicate.Test result be shown in the following table 1 and
In table 2.
1 Mn of table (8-HQ)2With tricyclazole and compounding to the virulence of Pyricularia oryzae
2 Mn of table (8-HQ)2With Prochloraz and compounding to the virulence of Pyricularia oryzae
Toxicity test result Tables 1 and 2 shows, the Mn (8-HQ) in the range of weight ratio 5:1-1:52With tricyclazole or miaow
The coefficient of synergism of fresh amine composition is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, prevention and treatment
Excellent effect and it is better than single dose kind.
2 Mn of embodiment (8-HQ)3It compounds with Fluoxastrobin, Prochloraz etc. to rice blast pathogen virulence
With Mn (8-HQ)3With Fluoxastrobin, Prochloraz be medicament to be measured, Pyricularia oryzae is subjects, according to above-mentioned test
Method carries out indoor bioassay, makees blank control with the processing without medicament, in triplicate.Test result be shown in the following table 3 and
In table 4.
3 Mn of table (8-HQ)3With Fluoxastrobin and compounding to the virulence of Pyricularia oryzae
4 Mn of table (8-HQ)3With Prochloraz and compounding to the virulence of Pyricularia oryzae
Toxicity test result table 3 and the display of table 4, the Mn (8-HQ) in the range of weight ratio 5:1-1:53With tricyclazole or miaow
The coefficient of synergism of fresh amine composition is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, prevention and treatment
Excellent effect and it is better than single dose product
3 Mn of embodiment (8-HQ)2It compounds with propiconazole, difenoconazole etc. to Rhizoctonia solani Kuhn virulence
With Mn (8-HQ)2With propiconazole, difenoconazole be medicament to be measured, rice sheath blight disease is subjects, according to upper
It states test method and carries out indoor bioassay, blank control is made with the processing without medicament, in triplicate.Test result is shown in down
In table 5 and table 6.
5 Mn of table (8-HQ)2With propiconazole and compounding to the virulence of Rhizoctonia solani Kuhn
6 Mn of table (8-HQ)2With difenoconazole and compounding to the virulence of Rhizoctonia solani Kuhn
Toxicity test result table 5 and the display of table 6, the Mn (8-HQ) in the range of weight ratio 10:1-1:102With propiconazole or
The coefficient of synergism of difenoconazole composition is all larger than 1.5, has apparent synergistic work to the prevention and control capability of Pyricularia oryzae
With control efficiency is excellent and is better than single dose kind.
4 Mn of embodiment (8-HQ)3It is compounded with metalaxyl, cyazofamid etc. and cucumber downy mildew spore germination is inhibited,
Using spore germination method, respectively by Mn (8-HQ)3, metalaxyl, cyazofamid dissolve with ethyl alcohol, then be watered and be diluted to
0.1, the suspension of 1,10,20,50,100,200mg/L.Fresh mould layer is taken to prepare spore suspension on the sick leaf of cucumber downy mildew
Liquid is mixed with institute dose out powders dilution, and every processing is repeated 6 times, and is placed in constant incubator (25 DEG C), and investigation spore is sprouted afterwards for 24 hours
Heat condition.Calculate the EC of each medicament50Value, the results are shown in Table 7 and table 8.
7 Mn of table (8-HQ)3With metalaxyl and compounding to the virulence of bacterium of downy mildew of cucumber spore
8 Mn of table (8-HQ)3With cyazofamid and compounding to the virulence of bacterium of downy mildew of cucumber spore
Toxicity test result table 7 and the display of table 8, the Mn (8-HQ) in the range of weight ratio 10:1-1:103With metalaxyl or
The coefficient of synergism of cyazofamid composition is all larger than 1.5, has obvious synergistic function to cucumber downy mildew spore germination, especially
It is at Mn (8-HQ)3With the weight ratio of metalaxyl or cyazofamid be 1:5 when synergistic effect it is most significant.
The anti-wheat sharp eyespot of 5 seed pelleting of embodiment,
Take the Mn (8-HQ) of 20g weight ratio 4:13With difenoconazole mixture, 8g dispersing agent 2700,5g wetting agent op-
10,0.3g xanthan gum, 2g aluminium-magnesium silicate, 4g ethylene glycol, 3g film forming agent NDSF, 2g rhodamine toner and 55.7g water are levigate in sand mill
It is 1~3 micron to particle average fineness, 20% suspension coating agent for seed is made.
During field trial, wheat seed is short by anti-58, coating pesticide-seeds ratio: 1:400,1:200,1:100 (weight
Than), wheat cultivation amount is 230kg/ha.After planting investigation in January investigation growth of seedling situation, does not find any symptom of chemical damage,
Emergence rate is all larger than 90%.Therefore, 20%Mn (8-HQ)3Emergence of the difenoconazole suspending seed-dressing agent to wheat, growth of seedling
It is safe.
Banded sclerotial blight incidence is investigated after wheat jointing, calculates disease index and control efficiency.20%Mn (8-HQ)3Benzene
Ether methyl cyclic-azole suspension seed-coating agent is in coating pesticide-seeds ratio: being respectively to sheath blight disease controlling effect under 1:400,1:200,1:100
60.2%, 80.7% and 89.3%.Therefore, 20%Mn (8-HQ)3Difenoconazole suspending seed-dressing agent is coated wheat seed can be with
Effectively prevent wheat sharp eyespot.
Claims (10)
1. a kind of composition with bactericidal activity；It is characterized in that, the composition with bactericidal activity is by least one
The mixture that kind (a) group compound and at least one (b) group compound form；It is metal shown in general formula A that (a), which organizes compound,
The chelate of manganese and 8-hydroxyquinoline (8-HQ) is Mn (8-HQ) as n=2 in general formula A2Chelate；As n=3 in general formula A
For Mn (8-HQ)3Chelate；
N=2 in general formula A, 3
It is metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole, Prochloraz, Fluoxastrobin, rice blast that (b), which organizes compound,
Spirit, kasugarnycin, fenoxanil, carbendazim, pyrimethanil, diethofencarb, fluazinam, Boscalid, cymoxanil, Tebuconazole, fluorine ring
Azoles, nitrile bacterium azoles, propiconazole, Cyproconazole, simeconazoles, triticonazole, Flusilazole, hexaconazole, kind bacterium azoles, difenoconazole, benzene oxygen
It is a kind of or several in bacterium amine, orysastrobin, fluoxastrobin, kresoxim-methyl, ZEN 90160, pyraclostrobin, trifloxystrobin and fluopicolide
Kind.
2. according to claim 1 with the composition of bactericidal activity；It is characterized in that, (a) organizes chemical combination in the mixture
The weight ratio of object and (b) combination of compounds is 100:1~1:100.
3. according to claim 1 with the composition of bactericidal activity；It is characterized in that, the mixture is processed into wettable
Pulvis, water dispersible granules, suspending agent and cream preparation；And use atomizers spray on the cauline leaf of plant any one of them,
To prevent and treat the disease of aboveground vegetation part, or seed clothing agent is processed into for seed pelleting, to promote seed quickly to emerge and prevent
Control the disease of foot end.
4. according to claim 1 with the composition of bactericidal activity；It is characterized in that, (a) group compound and (b) group
Compound is used in mixed way, and has significant synergistic function, is used to be used alone than it with more excellent when control of plant disease
Different bactericidal activity.
5. according to claim 1 with the composition of bactericidal activity；It is characterized in that, the composition belongs to broad spectrum activity
Fungicide effectively kills pathogenic oomycetes and fungi.
6. according to claim 1 with the composition of bactericidal activity；It is characterized in that, the prevention and treatment plant includes to water
Rice, wheat, the disease control of fruit and vegetables, fruit.
7. a kind of application method of the composition described in claim 1 with bactericidal activity；It is characterised in that it includes following step
It is rapid:
(1) bactericide compounded indoor toxicity test standard is determined using Plating, i.e., using Plating measurement medicament to pathogen
The inhibiting effect of mycelia growth；First by (a) group compound and (b) group compound bactericidal agent original medicinal alcohol dissolution, be configured to be
The medical fluid of column concentration is spare, is then added in the PDA culture medium for having melted and being cooled to room temperature medical fluid in 1% ratio, fills
Divide after shaking up, pours into the culture dish (Φ 90mm) of sterilizing and band medicine plate is made, sterile water is added to the PDA culture medium of room temperature
In, cultivate pathogen；By cultured pathogen, growing way one is selected with sterilization punchers (Φ 5mm) under aseptic technique
The bacterium colony of cause cuts bacteria cake from colony edge, with inoculator by pure culture biscuits involvng inoculation in band medicine plate center, mycelia face mycelia towards
Under, ware lid is covered, culture in 25 DEG C of constant incubator is placed in, blank control is made with the processing without medicament, culture is adjusted after 6 days
Pathogen hypha growth condition is looked into, the colony diameter of each processing is measured using crossing method, and using regression analysis to examination
It tests data to be analyzed, calculates each medicament to the EC of pathogen50, the virulence size of more each treatment agent, wherein EC50It is
Fungicidal activity evaluation index；Coefficient of synergism (SR) is calculated by formula (1) and formula (2):
(a) group compound synergy type mixed with (b) group compound bactericidal agent, synergy system are evaluated according to Wadley method
Number (SR)<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect；
(2) by (a) group compound, 100:1~1:100 is mixed according to above-mentioned weight ratio with (b) group compound, is used in combination, and add
Work is at wettable powder, water dispersible granules, suspending agent and cream preparation；Then any one of them is being planted using atomizers spray
On the cauline leaf of object, to prevent and treat the disease of aboveground vegetation part, or seed clothing agent is processed into for seed pelleting, to promote seed fast
The disease of speed emergence and prevention and treatment foot end；Then the synergy of two groups of compounds is calculated according to formula (1), formula (2)
Coefficient.
8. the application method of the composition with bactericidal activity according to claim 7；It is characterized in that, (a) groupization
It closes object and (b) group compound bactericidal agent is mixed to rice blast pathogen virulence, comprising:
Mn(8-HQ)2It compounds with tricyclazole, Prochloraz, propiconazole, difenoconazole to rice blast pathogen virulence, with Mn (8-
HQ)2It is medicament to be measured using Pyricularia oryzae as subjects with tricyclazole, Prochloraz, propiconazole, difenoconazole, according to above-mentioned
Test method carries out indoor bioassay, makees blank control with the processing without medicament, in triplicate；Test result is in weight
Than Mn in the range of 5:1-1:5 (8-HQ)2With the coefficient of synergism of tricyclazole, Prochloraz, propiconazole or difenoconazole composition
1.5 are all larger than, the prevention and control capability of Pyricularia oryzae is had obvious synergistic function, control efficiency is excellent and is better than single dose product
Kind；
Mn(8-HQ)3It compounds with Fluoxastrobin, Prochloraz etc. to rice blast pathogen virulence, with Mn (8-HQ)3With Fluoxastrobin, Prochloraz
It is subjects for medicament to be measured, Pyricularia oryzae, carries out indoor bioassay according to above-mentioned test method, is free of the place of medicament
Reason makees blank control, and test result is shown, the Mn (8-HQ) in the range of weight ratio 5:1-1:53With tricyclazole or Prochloraz group
The coefficient of synergism for closing object is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, control efficiency is excellent
It is different and be better than single dose product.
9. the application method of the composition with bactericidal activity according to claim 7；It is characterized in that, the Mn (8-HQ)3
Cucumber downy mildew spore germination is inhibited with metalaxyl, compounding for cyazofamid, using spore germination method, respectively by Mn (8-HQ)3、
Metalaxyl, cyazofamid are dissolved with ethyl alcohol, then are watered the suspension for being diluted to 0.1,1,10,20,50,100,200mg/L；In cucumber
It takes fresh mould layer to prepare spore suspension on the sick leaf of downy mildew, is mixed with institute dose out powders dilution, every processing is repeated 6 times, and is put
It sets in insulating box, spore germination situation is investigated in 25 DEG C of cultures afterwards for 24 hours, and toxicity test is the results show that in weight ratio 10:1-1:10
In the range of Mn (8-HQ)31.5 are all larger than with the coefficient of synergism of metalaxyl or cyazofamid composition, cucumber downy mildew spore is sprouted
Hair has obvious synergistic function, especially at Mn (8-HQ)3With the weight ratio of metalaxyl or cyazofamid be 1:5 when synergy make
With most significantly.
10. the application method of the composition with bactericidal activity according to claim 7；It is characterized in that, described kind of attached bag
The anti-wheat sharp eyespot of clothing, taking 20g weight ratio is the Mn (8-HQ) of 4:13With the mixture of difenoconazole, 8g dispersing agent 2700,
5g wetting agent op-10,0.3g xanthan gum, 2g aluminium-magnesium silicate, 4g ethylene glycol, 3g film forming agent NDSF, 2g rhodamine toner and 55.7g water exist
In sand mill it is levigate to average particle size be 1~3 micron, 20% suspension coating agent for seed is made；During field trial,
Wheat seed is short by anti-58, coating pesticide-seeds ratio: 1:400,1:200,1:100 (weight ratio), and wheat cultivation amount is 230kg/ha；It broadcasts
January investigates growth of seedling situation after kind, does not find that any symptom of chemical damage, emergence rate are all larger than 90%；Therefore, 20%Mn (8-
HQ)3Difenoconazole suspending coating agent for seed is safe to wheat；Banded sclerotial blight incidence, meter are investigated after wheat jointing
Calculate disease index and control efficiency；20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed is in coating pesticide-seeds ratio: 1:400,
It is respectively 60.2%, 80.7% and 89.3% to sheath blight disease controlling effect under 1:200,1:100；Therefore, 20%Mn (8-HQ)3Benzene
Ether methyl cyclic-azole suspension coating agent for seed coating wheat seed can effectively prevent wheat sharp eyespot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811246726.8A CN109221234A (en) | 2018-10-25 | 2018-10-25 | A kind of composition and its application method with bactericidal activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811246726.8A CN109221234A (en) | 2018-10-25 | 2018-10-25 | A kind of composition and its application method with bactericidal activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109221234A true CN109221234A (en) | 2019-01-18 |
Family
ID=65081780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811246726.8A Pending CN109221234A (en) | 2018-10-25 | 2018-10-25 | A kind of composition and its application method with bactericidal activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109221234A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109769829A (en) * | 2019-03-18 | 2019-05-21 | 湖南速博生物技术有限公司 | Bactericidal composition based on 8-hydroxyquinoline |
| CN111183998A (en) * | 2020-02-28 | 2020-05-22 | 吉林农业科技学院 | 8-hydroxyquinoline calcium-containing bactericidal composition for preventing and treating downy mildew and late blight |
| CN113396919A (en) * | 2021-07-19 | 2021-09-17 | 广东中迅农科股份有限公司 | Bactericidal composition and application thereof |
| CN115777722A (en) * | 2022-11-30 | 2023-03-14 | 华中农业大学 | Application of alexidine dihydrochloride to inhibition of phytopathogens |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1215676A (en) * | 1968-06-21 | 1970-12-16 | Nippon Kayaku Kk | Fungicidal compositions |
| CN101785464A (en) * | 2010-03-25 | 2010-07-28 | 青岛瀚生生物科技股份有限公司 | Oxine-copper composite bactericide |
| CN102369942A (en) * | 2011-10-19 | 2012-03-14 | 广东中迅农科股份有限公司 | Oxinecopper and prochloraz containing pesticide composite |
| CN104094939A (en) * | 2014-06-30 | 2014-10-15 | 中国水稻研究所 | Pesticide compound containing copper 8-hydroxyquinolinate and difenoconazole |
-
2018
- 2018-10-25 CN CN201811246726.8A patent/CN109221234A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1215676A (en) * | 1968-06-21 | 1970-12-16 | Nippon Kayaku Kk | Fungicidal compositions |
| CN101785464A (en) * | 2010-03-25 | 2010-07-28 | 青岛瀚生生物科技股份有限公司 | Oxine-copper composite bactericide |
| CN102369942A (en) * | 2011-10-19 | 2012-03-14 | 广东中迅农科股份有限公司 | Oxinecopper and prochloraz containing pesticide composite |
| CN104094939A (en) * | 2014-06-30 | 2014-10-15 | 中国水稻研究所 | Pesticide compound containing copper 8-hydroxyquinolinate and difenoconazole |
Non-Patent Citations (2)
| Title |
|---|
| HERMANG ERSHON等: "ynergistic Antifungal Action of 8-Quinolinol and Its Bischelate with Copper(ll) and with Mixed Ligand Chelates Composed of Copper(ll), 8-Quinolinol, and Aromatic Hydroxy Acids", 《JOURNAL OF PHARMACEUTICAL SCIENCES》 * |
| V. THAMILARASAN等: "Synthesis, molecular structure, theoretical calculation, DNA/protein interaction and cytotoxic activity of manganese(III) complex with 8-hydroxyquinoline", 《JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109769829A (en) * | 2019-03-18 | 2019-05-21 | 湖南速博生物技术有限公司 | Bactericidal composition based on 8-hydroxyquinoline |
| CN111183998A (en) * | 2020-02-28 | 2020-05-22 | 吉林农业科技学院 | 8-hydroxyquinoline calcium-containing bactericidal composition for preventing and treating downy mildew and late blight |
| CN111183998B (en) * | 2020-02-28 | 2021-08-24 | 吉林农业科技学院 | 8-hydroxyquinoline calcium-containing bactericidal composition for preventing and treating downy mildew and late blight |
| CN113396919A (en) * | 2021-07-19 | 2021-09-17 | 广东中迅农科股份有限公司 | Bactericidal composition and application thereof |
| CN115777722A (en) * | 2022-11-30 | 2023-03-14 | 华中农业大学 | Application of alexidine dihydrochloride to inhibition of phytopathogens |
| CN115777722B (en) * | 2022-11-30 | 2024-04-30 | 华中农业大学 | Application of alexidine dihydrochloride in inhibiting plant pathogenic bacteria |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109221234A (en) | A kind of composition and its application method with bactericidal activity | |
| CN104012579B (en) | A kind of bactericidal composition and application thereof | |
| CN106973907A (en) | A kind of composition pesticide containing copper 8-hydroxyquinolinate and kasugarnycin and its application | |
| CN108200910A (en) | A kind of composite bactericide | |
| CN109548787A (en) | A kind of bactericidal composition and its application | |
| CN108669080A (en) | A kind of bactericidal composition containing Boscalid and its application | |
| CN104488869B (en) | A kind of bactericidal composition of the ethyl phenylacrylate of 2 cyano group, 3 amino 3 and Osthole | |
| CN108770858A (en) | Pyraclostrobin is being prepared with probenazole for the purposes in preventing the fungicide of the plant disease caused by Rhizoctonia cerealis | |
| CN107372550A (en) | A kind of pesticide combination comprising folpet and benzo alkene fluorine bacterium azoles | |
| CN107333792A (en) | By rosickyite azoles and the bactericide of the preventing and treating rice blast of miaow chaff azoles compounding | |
| CN109832273B (en) | Application of branched chain amino acid in preparation of rice disease control agent | |
| CN106937636A (en) | The bactericidal composition of fluorine-containing azoles ring bacterium amine | |
| CN105794830A (en) | Pyraclostrobin, thiamethoxam and prochloraz compound suspended seed coating agent and preparation method thereof | |
| CN108849946A (en) | A kind of bactericidal composition and application thereof containing folpet and epoxiconazole | |
| CN104521992B (en) | Containing methylsulfonyl bacterium azoles and the complex composition of dicarboximide series bactericidal agent and preparation | |
| CN103798251A (en) | Compound pesticide containing triazolone and Saisentong and application thereof | |
| CN104522030B (en) | A kind of containing methylsulfonyl bacterium azoles and the complex composition of imidazoles antibacterial and preparation | |
| CN103814949B (en) | A kind of Recompounded pesticide containing triazolone and cuprous oxide | |
| CN108041063A (en) | A kind of bactericidal composition of containing cymoxanil and its application | |
| CN107960416A (en) | A kind of bactericidal composition and its application containing dimethomorph | |
| CN108041057B (en) | Compound composition for preventing and treating fruit tree diseases and application thereof | |
| CN107996581A (en) | A kind of composite bactericide | |
| CN114680121A (en) | Bactericidal composition containing hemin and S-abscisic acid | |
| CN105961421A (en) | Difenoconazole, propiconazole and pyraclostrobin compounded suspension and preparation method thereof | |
| CN105052998B (en) | A kind of complex composition and bactericide containing toxic fluoride phosphate and carbamate fungicide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190118 |
|
| RJ01 | Rejection of invention patent application after publication |