CN109221234A - A kind of composition and its application method with bactericidal activity - Google Patents

A kind of composition and its application method with bactericidal activity Download PDF

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Publication number
CN109221234A
CN109221234A CN201811246726.8A CN201811246726A CN109221234A CN 109221234 A CN109221234 A CN 109221234A CN 201811246726 A CN201811246726 A CN 201811246726A CN 109221234 A CN109221234 A CN 109221234A
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composition
seed
agent
bactericidal activity
disease
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杨代斌
闫晓静
袁会珠
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N55/00Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
    • A01N55/02Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46N-acyl derivatives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/501,3-Diazoles; Hydrogenated 1,3-diazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/541,3-Diazines; Hydrogenated 1,3-diazines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647Triazoles; Hydrogenated triazoles
    • A01N43/6531,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/38Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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Abstract

The invention discloses a kind of compositions and its application method with bactericidal activity for belonging to pesticide range;The composition with bactericidal activity is to organize compound and at least one (b) by least one (a) to organize the mixture that compound forms;Its (a) organizes the chelate Mn (8-HQ) that compound is manganese metal and 8-hydroxyquinoline (8-HQ) shown in general formula A, and (b) organizing compound is metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole etc.;It is mixed object and is processed into sprayable preparation;And using atomizers spray on the cauline leaf of plant, to prevent and treat the disease of aboveground vegetation part, or it is processed into seed clothing agent for seed pelleting, to promote seed quickly to emerge and prevent and treat the disease of foot end.Toxicity test is the results show that the coefficient of synergism of composition is all larger than 1.5;To rice, wheat, the disease control ability of vegetables and fruit is had obvious synergistic function, and is better than single dose kind.

Description

A kind of composition and its application method with bactericidal activity
Technical field
The invention belongs to pesticide range, in particular to a kind of composition and its application method with bactericidal activity.
Background technique
Crop disease is varied, and producing region distribution is different, and amblent air temperature is different, and disease species are also all kinds of.In crop The use of fungicide is the important measures for controlling disease and occurring during disease control, provides effective guarantor for stable crop yield high yield Barrier.But fungicide use often exist control efficiency it is undesirable and there are disease to fungicide generate resistance cause prevention and treatment imitate The problems such as fruit declines.For example, metalaxyl was once the control very effective fungicide of crop oomycetes disease, but lasting use for many years Cause Metalaxyl sensitivity development serious, metalaxyl is used alone now it is difficult to ensure that ideal control efficiency.In practical agriculture In industry production, it is to improve bactericidal agent for preventing and treating effect and delay germ to sterilization that the fungicide with synergistic function, which is used in mixed way, The effective means of agent resistance.
Summary of the invention
The object of the present invention is to provide a kind of composition and its application method with bactericidal activity;It is characterized in that, institute Stating the composition with bactericidal activity is to organize compound and at least one (b) by least one (a) to organize the mixing that compound forms Object;(a) organizes the chelate that compound is manganese metal and 8-hydroxyquinoline (8-HQ) shown in general formula A, as n=2 in general formula A For Mn (8-HQ)2Chelate;It is as n=3 in general formula A Mn (8-HQ)3Chelate;
(b) organize compound be metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole, Prochloraz, Fluoxastrobin, Isoprothiolane, kasugarnycin, fenoxanil, carbendazim, pyrimethanil, diethofencarb, fluazinam, Boscalid, cymoxanil, Tebuconazole, Epoxiconazole, nitrile bacterium azoles, propiconazole, Cyproconazole, simeconazoles, triticonazole, Flusilazole, hexaconazole, kind bacterium azoles, difenoconazole, It is a kind of in SSF 126, orysastrobin, fluoxastrobin, kresoxim-methyl, ZEN 90160, pyraclostrobin, trifloxystrobin and fluopicolide Or it is several.
It is 100:1~1:100 that (a), which organizes compound and the weight ratio of (b) combination of compounds, in the mixture.
The mixture is processed into wettable powder, water dispersible granules, suspending agent and cream preparation;And by any one of them Using atomizers spray on the cauline leaf of plant, to prevent and treat the disease of aboveground vegetation part, or be processed into seed clothing agent for kind Attached bag clothing, to promote seed quickly to emerge and prevent and treat the disease of foot end.
(a) organizes compound and is used in mixed way with (b) group compound, has significant synergistic function, is used for plant Being used alone when disease control than it has superior bactericidal activity.
The composition belongs to broad spectrum activity fungicide, effectively kills to pathogenic oomycetes and fungi.
The application method of the composition with bactericidal activity;It is characterised by comprising:
(1) bactericide compounded indoor toxicity test standard is determined using Plating, i.e., using Plating measurement medicament to disease The inhibiting effect of opportunistic pathogen mycelia growth;First by (a) group compound and the compound bactericidal agent original medicinal alcohol dissolution of (b) group, prepare It is spare at the medical fluid of series of concentrations, then medical fluid is added to the PDA culture medium for having melted and being cooled to room temperature in 1% ratio In, it after sufficiently shaking up, pours into the culture dish (Φ 90mm) of sterilizing and band medicine plate is made, the PDA that room temperature is added in sterile water is trained It supports in base, cultivates pathogen;By cultured pathogen, under aseptic technique with sterilization punchers (Φ 5mm) selection length The consistent bacterium colony of gesture, cuts bacteria cake from colony edge, with inoculator by pure culture biscuits involvng inoculation in band medicine plate center, mycelia face mycelia face Downwards, ware lid is covered, culture in 25 DEG C of constant incubator is placed in, blank control is made with the processing without medicament, after culture 6 days Pathogen hypha growth condition is investigated, the colony diameter of each processing is measured using crossing method, and utilize regression analysis pair Test data is analyzed, and calculates each medicament to the EC of pathogen50, the virulence size of more each treatment agent, wherein EC50 It is fungicidal activity evaluation index;Coefficient of synergism (SR) is calculated by formula (1) and formula (2):
(a) group compound synergy type mixed with (b) group compound bactericidal agent is evaluated according to Wadley method, is increased Effect coefficient (SR)<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect;
(2) by (a) group compound, 100:1~1:100 is mixed according to above-mentioned weight ratio with (b) group compound, is used in combination, And it is processed into wettable powder, water dispersible granules, suspending agent and cream preparation;Then any one of them is used into atomizers spray On the cauline leaf of plant, to prevent and treat the disease of aboveground vegetation part, or seed clothing agent is processed into for seed pelleting, to promote to plant The disease of sub quickly emergence and prevention and treatment foot end;Then the collaboration of two groups of compounds is calculated according to formula (1), formula (2) Coefficient of synergism,
The prevention and treatment plant includes to rice, wheat, the disease control of fruit and vegetables, fruit.
(a) organizes compound and (b) group compound bactericidal agent is mixed to rice blast pathogen virulence, comprising:
Mn(8-HQ)2It compounds with tricyclazole, Prochloraz, propiconazole, difenoconazole to rice blast pathogen virulence, with Mn (8-HQ)2It is medicament to be measured using Pyricularia oryzae as subjects with tricyclazole, Prochloraz, propiconazole, difenoconazole, according to upper It states test method and carries out indoor bioassay, blank control is made with the processing without medicament, in triplicate.Test result is in weight Amount is than Mn in the range of 5:1-1:5 (8-HQ)2With the synergy system of tricyclazole, Prochloraz, propiconazole or difenoconazole composition Number is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, control efficiency is excellent and is better than single dose Kind.
Mn(8-HQ)3It compounds with Fluoxastrobin, Prochloraz etc. to rice blast pathogen virulence, with Mn (8-HQ)3With Fluoxastrobin, miaow Fresh amine is medicament to be measured, Pyricularia oryzae is subjects, carries out indoor bioassay according to above-mentioned test method, to be free of medicament Processing make blank control, test result is shown, the Mn (8-HQ) in the range of weight ratio 5:1-1:53It is fresh with tricyclazole or miaow The coefficient of synergism of amine composition is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, prevention and treatment effect Fruit is excellent and is better than single dose product.
The Mn (8-HQ)3Cucumber downy mildew spore germination is inhibited with metalaxyl, compounding for cyazofamid, is sprouted using spore Hair method, respectively by Mn (8-HQ)3, metalaxyl, cyazofamid dissolved with ethyl alcohol, then be watered and be diluted to 0.1,1,10,20,50,100, The suspension of 200mg/L;Fresh mould layer is taken to prepare spore suspension on the sick leaf of cucumber downy mildew, with institute's dose out powders dilution Mixing, every processing are repeated 6 times, and are placed in insulating box, and spore germination situation is investigated in 25 DEG C of cultures afterwards for 24 hours, and toxicity test result is aobvious Show, the Mn (8-HQ) in the range of weight ratio 10:1-1:103It is all larger than with the coefficient of synergism of metalaxyl or cyazofamid composition 1.5, cucumber downy mildew spore germination is had obvious synergistic function, especially at Mn (8-HQ)3With metalaxyl or cyanogen frost Synergistic effect is most significant when the weight ratio of azoles is 1:5.
The anti-wheat sharp eyespot of seed pelleting, taking 20g weight ratio is the Mn (8-HQ) of 4:13With mixing for difenoconazole Close object, 8g dispersing agent 2700,5g wetting agent op-10,0.3g xanthan gum, 2g aluminium-magnesium silicate, 4g ethylene glycol, 3g film forming agent NDSF, It to average particle size is 1~3 micron that 2g rhodamine toner and 55.7g water are levigate in sand mill, and 20% suspension seed pelleting is made Agent;During field trial, wheat seed is short by anti-58, coating pesticide-seeds ratio: 1:400,1:200,1:100 (weight ratio), small Wheat application rate is 230kg/ha;After planting January investigates growth of seedling situation, does not find that any symptom of chemical damage, emergence rate are big In 90%;Therefore, 20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed is safe to wheat;In wheat jointing Banded sclerotial blight incidence is investigated afterwards, calculates disease index and control efficiency;20%Mn (8-HQ)3Difenoconazole suspending seed Coating agent is in coating pesticide-seeds ratio: being respectively 60.2%, 80.7% and to sheath blight disease controlling effect under 1:400,1:200,1:100 89.3%.Therefore, 20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed coating wheat seed can effectively prevent wheat Banded sclerotial blight.
The beneficial effects of the invention are as follows the present invention by mixed with (b) group compound bactericidal agent to (a) group compound, to water Rice, wheat, the disease virulence test of fruit and vegetables, fruit, toxicity test is the results show that the coefficient of synergism of composition is all larger than 1.5, the prevention and control capability of Pyricularia oryzae is had obvious synergistic function, control efficiency is excellent and is better than single dose product.To Huang Melon downy mildew spore germination has obvious synergistic function, especially at Mn (8-HQ)3With the weight of metalaxyl or cyazofamid Synergistic effect is most significant when than for 1:5.20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed is coated wheat seed to line Blight control efficiency is respectively 60.2%, 80.7% and 89.3%.Therefore, 20%Mn (8-HQ)3Difenoconazole suspending seed Coating agent coating wheat seed can effectively prevent wheat sharp eyespot;It is safe to the emergence of wheat, growth of seedling.
Specific embodiment
The present invention provides a kind of composition and its application method with bactericidal activity;The combination with bactericidal activity Object is to organize compound and at least one (b) by least one (a) to organize the mixture that compound forms;(a) organizes compound The chelate of manganese metal shown in general formula A and 8-hydroxyquinoline (8-HQ) is Mn (8-HQ) as n=2 in general formula A2Chelate;When It is when n=3 in general formula A Mn (8-HQ)3Chelate;
(b) organize compound be metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole, Prochloraz, Fluoxastrobin, Isoprothiolane, kasugarnycin, fenoxanil, carbendazim, pyrimethanil, diethofencarb, fluazinam, Boscalid, cymoxanil, Tebuconazole, Epoxiconazole, nitrile bacterium azoles, propiconazole, Cyproconazole, simeconazoles, triticonazole, Flusilazole, hexaconazole, kind bacterium azoles, difenoconazole, It is a kind of in SSF 126, orysastrobin, fluoxastrobin, kresoxim-methyl, ZEN 90160, pyraclostrobin, trifloxystrobin and fluopicolide Or it is several.
In order to better illustrate the present invention, it is further described below by embodiment.
One, embodiment determines bactericide compounded indoor toxicity test standard using Plating,
The test method of indoor bioassay: referring to People's Republic of China's agricultural industry criteria NY/T 1156.2- 2006, the inhibiting effect that pathogen mycelia is grown using Plating measurement medicament.(a) group compound and (b) are first organized into chemical combination The former medicinal alcohol of object fungicide dissolves, and the medical fluid for being configured to series of concentrations is spare;Then medical fluid is added in 1% ratio In the PDA culture medium for melting and being cooled to room temperature, after sufficiently shaking up, pours into the culture dish (Φ 90mm) of sterilizing to be made and be put down with medicine Plate, the processing of sterile water to the PDA culture medium for having melted and being cooled to room temperature is added as blank control, every processing repeats three It is secondary;By cultured pathogen, the consistent bacterium colony of growing way is selected with sterilization punchers (Φ 5mm) under aseptic technique, from Colony edge cuts bacteria cake, and with inoculator by pure culture biscuits involvng inoculation in band medicine plate center, mycelia face mycelia downwards, covers ware lid, It is placed in culture in 25 DEG C of constant incubator, blank control is made with the processing without medicament, in triplicate, culture is investigated after 6 days Pathogen hypha growth condition measures the colony diameter of each processing using crossing method, and using regression analysis to test Data are analyzed, and calculate each medicament to the EC50 of pathogen, the virulence size of more each treatment agent, wherein EC50It is to kill Microbial inoculum activity rating index;Coefficient of synergism (SR) is calculated by formula (1) and formula (2):
(a) group compound synergy type mixed with (b) group compound bactericidal agent is evaluated according to Wadley method, is increased Effect coefficient (SR)<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect.
Two, the bactericide compounded indoor virulence of bioassay,
Embodiment 1, Mn (8-HQ)2It compounds with tricyclazole, Prochloraz etc. to rice blast pathogen virulence,
With Mn (8-HQ)2With tricyclazole, Prochloraz be medicament to be measured, Pyricularia oryzae is subjects, according to above-mentioned test Method carries out indoor bioassay, makees blank control with the processing without medicament, in triplicate.Test result be shown in the following table 1 and In table 2.
1 Mn of table (8-HQ)2With tricyclazole and compounding to the virulence of Pyricularia oryzae
2 Mn of table (8-HQ)2With Prochloraz and compounding to the virulence of Pyricularia oryzae
Toxicity test result Tables 1 and 2 shows, the Mn (8-HQ) in the range of weight ratio 5:1-1:52With tricyclazole or miaow The coefficient of synergism of fresh amine composition is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, prevention and treatment Excellent effect and it is better than single dose kind.
2 Mn of embodiment (8-HQ)3It compounds with Fluoxastrobin, Prochloraz etc. to rice blast pathogen virulence
With Mn (8-HQ)3With Fluoxastrobin, Prochloraz be medicament to be measured, Pyricularia oryzae is subjects, according to above-mentioned test Method carries out indoor bioassay, makees blank control with the processing without medicament, in triplicate.Test result be shown in the following table 3 and In table 4.
3 Mn of table (8-HQ)3With Fluoxastrobin and compounding to the virulence of Pyricularia oryzae
4 Mn of table (8-HQ)3With Prochloraz and compounding to the virulence of Pyricularia oryzae
Toxicity test result table 3 and the display of table 4, the Mn (8-HQ) in the range of weight ratio 5:1-1:53With tricyclazole or miaow The coefficient of synergism of fresh amine composition is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, prevention and treatment Excellent effect and it is better than single dose product
3 Mn of embodiment (8-HQ)2It compounds with propiconazole, difenoconazole etc. to Rhizoctonia solani Kuhn virulence
With Mn (8-HQ)2With propiconazole, difenoconazole be medicament to be measured, rice sheath blight disease is subjects, according to upper It states test method and carries out indoor bioassay, blank control is made with the processing without medicament, in triplicate.Test result is shown in down In table 5 and table 6.
5 Mn of table (8-HQ)2With propiconazole and compounding to the virulence of Rhizoctonia solani Kuhn
6 Mn of table (8-HQ)2With difenoconazole and compounding to the virulence of Rhizoctonia solani Kuhn
Toxicity test result table 5 and the display of table 6, the Mn (8-HQ) in the range of weight ratio 10:1-1:102With propiconazole or The coefficient of synergism of difenoconazole composition is all larger than 1.5, has apparent synergistic work to the prevention and control capability of Pyricularia oryzae With control efficiency is excellent and is better than single dose kind.
4 Mn of embodiment (8-HQ)3It is compounded with metalaxyl, cyazofamid etc. and cucumber downy mildew spore germination is inhibited,
Using spore germination method, respectively by Mn (8-HQ)3, metalaxyl, cyazofamid dissolve with ethyl alcohol, then be watered and be diluted to 0.1, the suspension of 1,10,20,50,100,200mg/L.Fresh mould layer is taken to prepare spore suspension on the sick leaf of cucumber downy mildew Liquid is mixed with institute dose out powders dilution, and every processing is repeated 6 times, and is placed in constant incubator (25 DEG C), and investigation spore is sprouted afterwards for 24 hours Heat condition.Calculate the EC of each medicament50Value, the results are shown in Table 7 and table 8.
7 Mn of table (8-HQ)3With metalaxyl and compounding to the virulence of bacterium of downy mildew of cucumber spore
8 Mn of table (8-HQ)3With cyazofamid and compounding to the virulence of bacterium of downy mildew of cucumber spore
Toxicity test result table 7 and the display of table 8, the Mn (8-HQ) in the range of weight ratio 10:1-1:103With metalaxyl or The coefficient of synergism of cyazofamid composition is all larger than 1.5, has obvious synergistic function to cucumber downy mildew spore germination, especially It is at Mn (8-HQ)3With the weight ratio of metalaxyl or cyazofamid be 1:5 when synergistic effect it is most significant.
The anti-wheat sharp eyespot of 5 seed pelleting of embodiment,
Take the Mn (8-HQ) of 20g weight ratio 4:13With difenoconazole mixture, 8g dispersing agent 2700,5g wetting agent op- 10,0.3g xanthan gum, 2g aluminium-magnesium silicate, 4g ethylene glycol, 3g film forming agent NDSF, 2g rhodamine toner and 55.7g water are levigate in sand mill It is 1~3 micron to particle average fineness, 20% suspension coating agent for seed is made.
During field trial, wheat seed is short by anti-58, coating pesticide-seeds ratio: 1:400,1:200,1:100 (weight Than), wheat cultivation amount is 230kg/ha.After planting investigation in January investigation growth of seedling situation, does not find any symptom of chemical damage, Emergence rate is all larger than 90%.Therefore, 20%Mn (8-HQ)3Emergence of the difenoconazole suspending seed-dressing agent to wheat, growth of seedling It is safe.
Banded sclerotial blight incidence is investigated after wheat jointing, calculates disease index and control efficiency.20%Mn (8-HQ)3Benzene Ether methyl cyclic-azole suspension seed-coating agent is in coating pesticide-seeds ratio: being respectively to sheath blight disease controlling effect under 1:400,1:200,1:100 60.2%, 80.7% and 89.3%.Therefore, 20%Mn (8-HQ)3Difenoconazole suspending seed-dressing agent is coated wheat seed can be with Effectively prevent wheat sharp eyespot.

Claims (10)

1. a kind of composition with bactericidal activity;It is characterized in that, the composition with bactericidal activity is by least one The mixture that kind (a) group compound and at least one (b) group compound form;It is metal shown in general formula A that (a), which organizes compound, The chelate of manganese and 8-hydroxyquinoline (8-HQ) is Mn (8-HQ) as n=2 in general formula A2Chelate;As n=3 in general formula A For Mn (8-HQ)3Chelate;
N=2 in general formula A, 3
It is metalaxyl, Propamocarb, cyazofamid, benzene metsulfovax, tricyclazole, Prochloraz, Fluoxastrobin, rice blast that (b), which organizes compound, Spirit, kasugarnycin, fenoxanil, carbendazim, pyrimethanil, diethofencarb, fluazinam, Boscalid, cymoxanil, Tebuconazole, fluorine ring Azoles, nitrile bacterium azoles, propiconazole, Cyproconazole, simeconazoles, triticonazole, Flusilazole, hexaconazole, kind bacterium azoles, difenoconazole, benzene oxygen It is a kind of or several in bacterium amine, orysastrobin, fluoxastrobin, kresoxim-methyl, ZEN 90160, pyraclostrobin, trifloxystrobin and fluopicolide Kind.
2. according to claim 1 with the composition of bactericidal activity;It is characterized in that, (a) organizes chemical combination in the mixture The weight ratio of object and (b) combination of compounds is 100:1~1:100.
3. according to claim 1 with the composition of bactericidal activity;It is characterized in that, the mixture is processed into wettable Pulvis, water dispersible granules, suspending agent and cream preparation;And use atomizers spray on the cauline leaf of plant any one of them, To prevent and treat the disease of aboveground vegetation part, or seed clothing agent is processed into for seed pelleting, to promote seed quickly to emerge and prevent Control the disease of foot end.
4. according to claim 1 with the composition of bactericidal activity;It is characterized in that, (a) group compound and (b) group Compound is used in mixed way, and has significant synergistic function, is used to be used alone than it with more excellent when control of plant disease Different bactericidal activity.
5. according to claim 1 with the composition of bactericidal activity;It is characterized in that, the composition belongs to broad spectrum activity Fungicide effectively kills pathogenic oomycetes and fungi.
6. according to claim 1 with the composition of bactericidal activity;It is characterized in that, the prevention and treatment plant includes to water Rice, wheat, the disease control of fruit and vegetables, fruit.
7. a kind of application method of the composition described in claim 1 with bactericidal activity;It is characterised in that it includes following step It is rapid:
(1) bactericide compounded indoor toxicity test standard is determined using Plating, i.e., using Plating measurement medicament to pathogen The inhibiting effect of mycelia growth;First by (a) group compound and (b) group compound bactericidal agent original medicinal alcohol dissolution, be configured to be The medical fluid of column concentration is spare, is then added in the PDA culture medium for having melted and being cooled to room temperature medical fluid in 1% ratio, fills Divide after shaking up, pours into the culture dish (Φ 90mm) of sterilizing and band medicine plate is made, sterile water is added to the PDA culture medium of room temperature In, cultivate pathogen;By cultured pathogen, growing way one is selected with sterilization punchers (Φ 5mm) under aseptic technique The bacterium colony of cause cuts bacteria cake from colony edge, with inoculator by pure culture biscuits involvng inoculation in band medicine plate center, mycelia face mycelia towards Under, ware lid is covered, culture in 25 DEG C of constant incubator is placed in, blank control is made with the processing without medicament, culture is adjusted after 6 days Pathogen hypha growth condition is looked into, the colony diameter of each processing is measured using crossing method, and using regression analysis to examination It tests data to be analyzed, calculates each medicament to the EC of pathogen50, the virulence size of more each treatment agent, wherein EC50It is Fungicidal activity evaluation index;Coefficient of synergism (SR) is calculated by formula (1) and formula (2):
(a) group compound synergy type mixed with (b) group compound bactericidal agent, synergy system are evaluated according to Wadley method Number (SR)<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect;
(2) by (a) group compound, 100:1~1:100 is mixed according to above-mentioned weight ratio with (b) group compound, is used in combination, and add Work is at wettable powder, water dispersible granules, suspending agent and cream preparation;Then any one of them is being planted using atomizers spray On the cauline leaf of object, to prevent and treat the disease of aboveground vegetation part, or seed clothing agent is processed into for seed pelleting, to promote seed fast The disease of speed emergence and prevention and treatment foot end;Then the synergy of two groups of compounds is calculated according to formula (1), formula (2) Coefficient.
8. the application method of the composition with bactericidal activity according to claim 7;It is characterized in that, (a) groupization It closes object and (b) group compound bactericidal agent is mixed to rice blast pathogen virulence, comprising:
Mn(8-HQ)2It compounds with tricyclazole, Prochloraz, propiconazole, difenoconazole to rice blast pathogen virulence, with Mn (8- HQ)2It is medicament to be measured using Pyricularia oryzae as subjects with tricyclazole, Prochloraz, propiconazole, difenoconazole, according to above-mentioned Test method carries out indoor bioassay, makees blank control with the processing without medicament, in triplicate;Test result is in weight Than Mn in the range of 5:1-1:5 (8-HQ)2With the coefficient of synergism of tricyclazole, Prochloraz, propiconazole or difenoconazole composition 1.5 are all larger than, the prevention and control capability of Pyricularia oryzae is had obvious synergistic function, control efficiency is excellent and is better than single dose product Kind;
Mn(8-HQ)3It compounds with Fluoxastrobin, Prochloraz etc. to rice blast pathogen virulence, with Mn (8-HQ)3With Fluoxastrobin, Prochloraz It is subjects for medicament to be measured, Pyricularia oryzae, carries out indoor bioassay according to above-mentioned test method, is free of the place of medicament Reason makees blank control, and test result is shown, the Mn (8-HQ) in the range of weight ratio 5:1-1:53With tricyclazole or Prochloraz group The coefficient of synergism for closing object is all larger than 1.5, has obvious synergistic function to the prevention and control capability of Pyricularia oryzae, control efficiency is excellent It is different and be better than single dose product.
9. the application method of the composition with bactericidal activity according to claim 7;It is characterized in that, the Mn (8-HQ)3 Cucumber downy mildew spore germination is inhibited with metalaxyl, compounding for cyazofamid, using spore germination method, respectively by Mn (8-HQ)3、 Metalaxyl, cyazofamid are dissolved with ethyl alcohol, then are watered the suspension for being diluted to 0.1,1,10,20,50,100,200mg/L;In cucumber It takes fresh mould layer to prepare spore suspension on the sick leaf of downy mildew, is mixed with institute dose out powders dilution, every processing is repeated 6 times, and is put It sets in insulating box, spore germination situation is investigated in 25 DEG C of cultures afterwards for 24 hours, and toxicity test is the results show that in weight ratio 10:1-1:10 In the range of Mn (8-HQ)31.5 are all larger than with the coefficient of synergism of metalaxyl or cyazofamid composition, cucumber downy mildew spore is sprouted Hair has obvious synergistic function, especially at Mn (8-HQ)3With the weight ratio of metalaxyl or cyazofamid be 1:5 when synergy make With most significantly.
10. the application method of the composition with bactericidal activity according to claim 7;It is characterized in that, described kind of attached bag The anti-wheat sharp eyespot of clothing, taking 20g weight ratio is the Mn (8-HQ) of 4:13With the mixture of difenoconazole, 8g dispersing agent 2700, 5g wetting agent op-10,0.3g xanthan gum, 2g aluminium-magnesium silicate, 4g ethylene glycol, 3g film forming agent NDSF, 2g rhodamine toner and 55.7g water exist In sand mill it is levigate to average particle size be 1~3 micron, 20% suspension coating agent for seed is made;During field trial, Wheat seed is short by anti-58, coating pesticide-seeds ratio: 1:400,1:200,1:100 (weight ratio), and wheat cultivation amount is 230kg/ha;It broadcasts January investigates growth of seedling situation after kind, does not find that any symptom of chemical damage, emergence rate are all larger than 90%;Therefore, 20%Mn (8- HQ)3Difenoconazole suspending coating agent for seed is safe to wheat;Banded sclerotial blight incidence, meter are investigated after wheat jointing Calculate disease index and control efficiency;20%Mn (8-HQ)3Difenoconazole suspending coating agent for seed is in coating pesticide-seeds ratio: 1:400, It is respectively 60.2%, 80.7% and 89.3% to sheath blight disease controlling effect under 1:200,1:100;Therefore, 20%Mn (8-HQ)3Benzene Ether methyl cyclic-azole suspension coating agent for seed coating wheat seed can effectively prevent wheat sharp eyespot.
CN201811246726.8A 2018-10-25 2018-10-25 A kind of composition and its application method with bactericidal activity Pending CN109221234A (en)

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