CN109832273B - Application of branched chain amino acid in preparation of rice disease control agent - Google Patents
Application of branched chain amino acid in preparation of rice disease control agent Download PDFInfo
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- CN109832273B CN109832273B CN201910178182.4A CN201910178182A CN109832273B CN 109832273 B CN109832273 B CN 109832273B CN 201910178182 A CN201910178182 A CN 201910178182A CN 109832273 B CN109832273 B CN 109832273B
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Abstract
The invention relates to the technical field of compounds, in particular to an application of branched chain amino acid in preparing a rice disease control medicament and a rice disease control medicament. The branched chain amino acid can effectively inhibit the infection of ustilaginoidea virens, the formation of ustilaginoidea virens and the generation of ustilaginoidea virens, and reduce the incidence rate of ustilaginoidea virens of rice, thereby effectively ensuring the growth of the rice and the formation of rice ears, reducing the generation of the ustilaginoidea virens, and improving the yield and the quality of the rice. The pesticide for preventing and treating rice diseases provided by the invention takes the branched chain amino acid provided by the invention as an effective component for preventing and treating rice false smut, can effectively inhibit invasion, growth, reproduction and toxin generation of the rice false smut, and reduces the morbidity of the rice false smut, thereby effectively ensuring the growth of rice and the formation of rice ears, reducing the generation of the rice false smut and improving the yield and the quality of the rice.
Description
Technical Field
The invention relates to the technical field of compounds, in particular to application of branched chain amino acid in preparation of a rice disease control medicament and a rice disease control medicament.
Background
The rice false smut is a fungal disease which is attacked in the ear stage of rice, and is characterized in that greenish black rice koji balls larger than rice grains are formed at the ear part of the rice, the incidence rate of the rice is obviously increased in recent years, and the disease is difficult to prevent and treat once infected, so that the yield of the rice is reduced, the rice false smut is also generated, and poisoning phenomena such as visceral diseases and the like can occur after people and livestock eat the rice false smut.
Therefore, the technical personnel in the field need to develop a novel rice false smut prevention and control agent to inhibit the increment of rice false smut, reduce the morbidity of the rice false smut, ensure the healthy growth of rice, reduce the toxin pollution of farmlands and improve the food safety.
Disclosure of Invention
The invention aims to provide the application of branched chain amino acid in preparing a rice disease control medicament, so as to effectively inhibit the growth of ustilaginoidea virens and the generation of toxins, reduce the incidence rate of ustilaginoidea virens and ensure the normal growth of rice, thereby improving the yield and the quality of the rice.
The invention also aims to provide a rice disease control agent, which can inhibit the growth of rice false smut and the generation of toxin, reduce the incidence rate of the rice false smut, effectively reduce the field toxin pollution and ensure the normal growth of rice, thereby improving the yield and the quality of the rice and ensuring the food safety.
The embodiment of the invention is realized by the following steps:
the application of branched chain amino acid in preparing rice disease preventing and treating medicine.
A rice disease control agent comprises branched chain amino acids and a carrier.
The embodiment of the invention has the beneficial effects that:
the branched chain amino acid can effectively inhibit the infection of ustilaginoidea virens, the formation of ustilaginoidea virens and the generation of ustilaginoidea virens, and reduce the incidence rate of ustilaginoidea virens, thereby effectively ensuring the growth of the paddy rice, the formation of the rice ears, reducing the generation of the ustilaginoidea virens and improving the yield and the quality of the paddy rice. The pesticide for preventing and treating rice diseases provided by the invention takes the branched chain amino acid provided by the invention as an effective component for preventing and treating rice false smut, can effectively inhibit invasion, growth, reproduction and toxin generation of the rice false smut, and reduces the morbidity of the rice false smut, thereby effectively ensuring the growth of rice and the formation of rice ears, reducing the generation of the rice false smut and improving the yield and the quality of the rice.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a line graph showing the growth inhibitory effect of L-leucine on Ustilaginoidea virens;
FIG. 2 is a line graph showing the growth inhibitory effect of L-isoleucine on Ustilaginoidea virens;
FIG. 3 is a line graph showing the growth inhibitory effect of valine on Ustilaginoidea virens;
FIG. 4 is a bar graph showing the growth inhibitory effect of branched chain amino acid pairing Ustilaginoidea virens;
FIG. 5 is a bar graph showing the effect of L-leucine on the number of rice koji balls of different rice varieties;
FIG. 6 is a bar graph of the effect of L-isoleucine on the number of rice koji balls of different rice varieties;
FIG. 7 is a bar graph showing the effect of valine on the number of koji balls of different rice cultivars.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The following will specifically describe the application of the branched-chain amino acids of the embodiments of the present invention in the preparation of a control agent for rice diseases and a control agent for rice diseases.
The false smut is a rice ear stage fungal disease caused by sclerotinia virginiana, which is also called false smut and green smut and is commonly called high-yield fruit in rice planting areas of various provinces in the Yangtze river basin, the North river and the south of China in China. When the ears of rice are taken out and the rice is bloomed, pathogenic bacteria invade flower organs and young glumes and parasitize in rice glumes, the pathogenic bacteria breed and grow in the glumes, grains are expanded and cracked, then the whole glumes are wrapped to form rice yeast ball diseased grains which are 3-4 times larger than healthy grains, the surfaces of the rice yeast balls at the early stage are smooth, dark green chlamydospores or black flat sclerotia are distributed by cracking at the later stage, the rice yeast balls fall into soil after being beaten by wind and rain, chlamydospores are generated by germination in the next year, and the chlamydospores are generated to be primarily infected with ascospores. In the heading and flowering period of rice, the occurrence of false smut is facilitated. Early heading varieties are relatively mild in morbidity, hybrid high-yield rice is relatively severe in morbidity, and false smut is aggravated by using excessive nitrogen fertilizer. False smut not only affects the yield of rice in producing areas, increases the blighted grain rate, the green rice rate and the broken rice rate, reduces the yield of local fields by 20-30 percent, can generate mycotoxin with strong toxicity to people and livestock, but also can cause poisoning to people and livestock when 0.5 percent of diseased grains are contained in the rice.
Because the disease is difficult to reverse once occurring, at present, in the aspect of false smut prevention and control, a plant protection policy of 'prevention and control is taken as a main part and comprehensive prevention and control' is taken as a guide, comprehensive prevention and control measures are taken for susceptible hybrid rice and disease-resistant japonica rice, cultivation management is enhanced, triadimefon, carbendazim or validamycin and other medicaments are sprayed before the breach to prevent the disease of the false smut, the loss is reduced to the minimum, but no branched chain amino acid is reported to be applied to the prevention and control of the false smut of rice so far.
In the embodiment, the application of the branched-chain amino acid in preparing the rice disease control medicament is provided, the rice disease control medicament is prepared by taking the branched-chain amino acid as a main active ingredient, so that the growth and toxin generation of ustilaginoidea virens of rice are effectively inhibited, the morbidity of ustilaginoidea virens of rice is reduced, the normal growth of rice is ensured, and the yield and the quality of rice are improved.
Wherein, the rice diseases include but are not limited to rice fungal diseases, rice bacterial diseases and rice viral diseases.
Wherein, the rice fungal diseases include but are not limited to rice blast, sheath blight, flax leaf spot, sclerotinia sclerotiorum, bakanae disease, downy mildew, false smut, leaf sheath rot and the like. Bacterial diseases of rice include, but are not limited to, bacterial blight, bacterial leaf streak, bacterial basal rot, and the like. The rice virus diseases include but are not limited to stripe disease, black-streaked dwarf disease and the like.
The research of the inventor finds that the rice disease control agent prepared by taking the branched chain amino acid as the main active ingredient can effectively inhibit the rice disease, particularly has obvious inhibition effect on false smut in rice fungal diseases, and can effectively inhibit the incidence of the false smut of the rice, thereby effectively ensuring the growth of the rice and improving the yield and the quality of the rice. It is to be understood that the control agent for rice diseases provided in the present embodiment can also be used for inhibiting other types of rice diseases.
In this embodiment, the branched-chain amino acids include one or more of L-leucine, L-isoleucine, and valine.
Wherein, the L-leucine has the following structural formula:
l-isoleucine has the following structural formula:
valine has the following structural formula:
in addition, the application also provides a control agent for rice diseases, which comprises branched chain amino acid and a carrier.
In the agent for controlling rice diseases provided by the present invention, the branched-chain amino acid may be in any form, such as solid, semisolid, liquid, aerosol, or the like.
Wherein the branched chain amino acid accounts for 0.05 to 32 percent of the weight of the rice disease control agent.
As noted above, branched chain amino acids include one or more of L-leucine, L-isoleucine, and valine. Wherein when the preventing and treating agent for rice diseases contains L-leucine, the L-leucine accounts for 0.05-2.4% of the preventing and treating agent for rice diseases by weight percent; when the preventing and treating agent for rice diseases contains L-isoleucine, the L-isoleucine accounts for 0.05-32% of the weight of the preventing and treating agent for rice diseases; when the rice disease control agent contains valine, the valine accounts for 0.05-16% of the weight of the rice disease control agent.
In a typical but non-limiting embodiment of the present invention, in the agent for controlling rice false smut, the L-leucine content is, for example, any one of 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, or 2.4% or a value in a range between any two, the L-isoleucine content is, for example, any one of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16%, or 32% or a value in a range between any two, the valine content is, for example, any one of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, or 16% or a value in a range between any two, the incidence of rice false smut can be reduced, and the higher the branched amino acid concentration is the better in controlling effect.
In a typical but non-limiting embodiment of the present invention, the rice false smut control agent is made of a mixture of any two of L-leucine, L-isoleucine and valine, for example, containing L-leucine and L-isoleucine, L-leucine and valine, or L-isoleucine and valine.
Further, in the above complex formulation, the content of L-leucine is 0.05% to 2.4%, preferably 0.4% to 1.6%, the content of L-isoleucine is 0.05% to 32%, preferably 0.5% to 4%, and the content of valine is 0.05% to 16%, preferably 0.5% to 4%. Specifically, the complex formulation may include: 0.4% of L-leucine and 0.5% of L-isoleucine, 0.4% of L-leucine and 4% of L-isoleucine, 1.6% of L-leucine and 0.5% of L-isoleucine, 1.6% of L-leucine and 4% of L-isoleucine, 0.4% of L-leucine and 0.5% of valine, 0.4% of L-leucine and 4% of valine, 1.6% of L-leucine and 0.5% of valine, 1.6% of L-leucine and 4% of valine, 0.5% of L-isoleucine and 0.5% of valine, 0.5% of L-isoleucine and 4% of valine, 1.6% of L-isoleucine and 0.5% of valine, and can reduce the incidence rate of rice false smut.
In a typical but non-limiting embodiment of the present invention, the rice false smut control agent may also be made of a mixture of three of L-leucine, L-isoleucine and valine.
The pesticide for preventing and treating rice diseases provided by the application can also comprise a carrier of branched chain amino acid. Including, for example, but not limited to, one or more of water, glucose, lactose, acacia, gelatin, mannitol, starch, magnesium trisilicate, talc, corn starch, keratin, colloidal silicon dioxide, potato starch, sandy soil, and nano-titanium dioxide, and may also include other carriers used in preparing formulations in solid, semi-solid, liquid, or aerosol form.
Optionally, the agent for controlling rice diseases further comprises auxiliary materials, and the auxiliary materials include but are not limited to one or more of a stabilizer, a thickener, a colorant and a perfume.
Optionally, the agent for controlling rice diseases further comprises a pesticide including, but not limited to, one or more of oxime tebuconazole, hexaconazole, benzyl propiconazole, azoxystrobin, validamycin, diniconazole, difenoconazole, triadimefon, tebuconazole, validamycin, thifluzamide and propiconazole.
The invention also provides a use method of the rice disease control agent, namely, the rice disease control agent is sprayed on rice ears.
The rice false smut prevention and treatment agent provided by the invention takes the branched chain amino acid provided by the invention as an effective component for preventing and treating rice false smut, can effectively inhibit invasion, growth, reproduction and toxin generation of the rice false smut, and reduces the incidence rate of the rice false smut, thereby effectively ensuring the growth of rice and the formation of rice ears, reducing the generation of the rice false smut and improving the yield and the quality of the rice.
The application of the branched-chain amino acid of the present invention in the preparation of a rice disease control agent and a rice disease control agent will be further described below with reference to examples.
Examples 1 to 7
Examples 1 to 7 provide a rice disease control agent whose main ingredient is L-leucine and water as a solvent. The concentrations of L-leucine in examples 1-7 were made to be 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 8 to 17
Examples 8 to 17 provide a rice disease control agent containing L-isoleucine as a main ingredient and water as a solvent. The concentrations of L-isoleucine in examples 8-17 were formulated to be 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Examples 18 to 26
Examples 18 to 26 provide a rice disease control agent containing valine as a main component and water as a solvent. The valine concentrations in examples 18 to 26 were made 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Examples 27 to 34
Examples 27 to 34 provide a rice disease control agent obtained by compounding 15 g/acre of 75% oxime bacterium tebuconazole (WDG) with L-leucine at different concentrations. In examples 27 to 34, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 35 to 41
Examples 35 to 41 provide a rice disease control agent obtained by compounding 15 g/acre of 43% tebuconazole (SC) with L-leucine at different concentrations. In examples 35 to 41, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 42 to 48
Examples 42 to 48 provide a rice disease control agent obtained by compounding 30% hexaconazole (SC)22.5 g/acre with L-leucine at different concentrations. In examples 42 to 48, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 49 to 55
Examples 49 to 55 provide a rice disease control agent obtained by compounding 15% propiconazole (SC) at 30 g/acre with L-leucine at different concentrations. In examples 49 to 55, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 56 to 62
Examples 56 to 62 provide a control agent for rice diseases, which is obtained by compounding 32.5% azoxystrobin (SC)60 g/acre with L-leucine at different concentrations. In examples 56 to 62, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 63 to 69
Examples 63 to 69 provide a rice disease control agent obtained by compounding 61% validamycin (SP)8.196 g/acre with L-leucine at different concentrations. In examples 63 to 69, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 70 to 76
Examples 70 to 76 provide a rice disease control agent obtained by compounding 45 g/acre of 12.5% diniconazole (WP) with L-leucine at different concentrations. In examples 70 to 76, the weight percentages of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 77 to 83
Examples 77 to 83 provide a rice disease control agent obtained by compounding 10% difenoconazole (WP)30 g/acre with L-leucine at different concentrations. In examples 77 to 83, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 84 to 90
Examples 84 to 90 provide a rice disease control agent obtained by compounding 15% triazolone (WP) at 100 g/acre with L-leucine at different concentrations. In examples 84 to 90, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 91 to 97
Examples 91 to 97 provide a control agent for rice diseases, which is obtained by compounding 21.5% tebuconazole (OD)30 g/acre with L-leucine at different concentrations. In examples 91 to 97, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Examples 98 to 104
Examples 98 to 104 provide a rice disease control agent obtained by compounding 15% validamycin (OD)33.3 g/acre with L-leucine at different concentrations. In examples 98 to 104, the percentages by weight of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Example 105-
Example 105-111 provides a control agent for rice diseases, which is prepared by formulating 12% thifluzamide (OD)46 g/acre with L-leucine at different concentrations. In example 105-111, the weight percentages of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Example 112-
Example 112-118 provides a rice disease control agent prepared by compounding 25% propiconazole (EC)60 mL/acre with L-leucine at different concentrations. In example 112-118, the weight percentages of L-leucine were 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, and 2.4%, respectively.
Example 119-
Example 119-128 provides a rice disease control agent, which is prepared by compounding 75% oxime-tebuconazole (WDG)15 g/acre with L-isoleucine at different concentrations. Wherein, in example 119-128, the weight percentages of L-isoleucine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 129-138
Example 129-138 provides a control agent for rice diseases, which is prepared by formulating 15 g/acre of 43% tebuconazole (SC) with L-isoleucine at different concentrations. Wherein, in example 129-138, the weight percentages of L-isoleucine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 139-
Example 139-148 provides a rice disease control agent, which is prepared by formulating 30% hexaconazole (SC)22.5 g/acre with L-isoleucine at different concentrations. In example 139-148, the weight percentages of L-isoleucine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 149-
Example 149-158 provides a rice disease-controlling agent prepared by compounding 15% propiconazole (SC)30 g/acre with L-isoleucine at different concentrations. In example 149-158, the weight percentages of L-isoleucine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 159-
Example 159-168 provides a rice disease control agent, which is prepared by compounding 32.5% azoxystrobin (SC)60 g/acre with L-isoleucine at different concentrations. In example 159-168, the weight percentages of L-isoleucine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 169-
Example 169- "178" provides a control agent for rice diseases, which is obtained by formulating 61% validamycin (SP)8.196 g/acre with L-isoleucine at different concentrations. In example 169-178, the weight percentages of L-isoleucine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 179-188
Example 179-188 provides a control agent for rice diseases, which is formulated with 45 g/acre of 12.5% diniconazole (WP) and L-isoleucine at different concentrations. In example 179-188, the weight percentages of L-isoleucine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 189-
Example 189-198 provides a control agent for rice diseases, which is prepared by compounding 10% difenoconazole (WP)30 g/mu with L-isoleucine at different concentrations. In example 189-.
Example 199-
Example 199-208 provides a control agent for rice diseases, which is prepared by compounding 15% triazolone (WP) at 100 g/acre with L-isoleucine at different concentrations. In example 199-208, the weight percentages of L-isoleucine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 209-
Example 209-218 provides a rice disease control agent, which is prepared by formulating 21.5% tebuconazole (OD)30 g/acre with L-isoleucine at different concentrations. In example 209-218, the weight percentages of L-isoleucine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 219-
Example 219-228 provides a control agent for rice diseases, which is obtained by compounding 15% validamycin (OD)33.3 g/acre with L-isoleucine at different concentrations. In example 219-228, the weight percentages of L-isoleucine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively.
Example 229-
Example 229-238 provides a rice disease control agent, which is prepared by formulating 12% thifluzamide (OD)46 g/acre with L-isoleucine at different concentrations. In example 229-.
Example 239-
Example 239-248 provides a rice disease control agent, which is obtained by compounding 25% propiconazole (EC)60 mL/acre with L-isoleucine at different concentrations. In example 239-.
Example 249-
Example 249-257 provides a control agent for rice diseases, which is prepared by compounding 75% oxime tebuconazole (WDG)15 g/acre with valine of different concentrations. In example 249-257, valine accounts for 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16% by weight, respectively.
Example 258-
Example 258-266 provides a control agent for rice diseases, which is prepared by combining 15 g/acre of 43% tebuconazole (SC) with valine of different concentrations. In example 258-266, the weight percentages of valine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 267-
Example 267-275 provides a control agent for rice diseases, which is formulated with 30% hexaconazole (SC)22.5 g/acre and valine of different concentrations. In example 267-275, the weight percentages of valine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 276-
Example 276-284 provides a rice disease control agent prepared by compounding 15% propiconazole (SC)30 g/acre with valine at different concentrations. In example 276-284, the weight percentages of valine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 285-
Example 285-293 provides a control agent for rice diseases, which is prepared by compounding 32.5% azoxystrobin (SC)60 g/acre with valine at different concentrations. In example 285-293, the weight percentages of valine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 294-
Example 294-302 provides a control agent for rice diseases, which is obtained by compounding 61% validamycin (SP)8.196 g/acre with valine of various concentrations. In example 294-.
Example 303-
Example 303-311 provides a control agent for rice diseases, which is prepared by compounding 45 g/acre of 12.5% diniconazole (WP) with valine at different concentrations. In example 303-311, the weight percentages of valine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 312-
Example 312-320 provides a rice disease control agent, which is formulated with 10% difenoconazole (WP)30 g/acre and valine of different concentrations. In example 312-320, the weight percentages of valine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 321-329
Example 321-329 provides a control agent for rice diseases, which is prepared by compounding 15% triazolone (WP) at 100 g/acre with valine at different concentrations. In example 321-329, the weight percentages of valine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 330-
Example 330-338 provides a control agent for rice diseases, which is prepared by formulating 30 g/acre of 21.5% tebuconazole (OD) with valine at different concentrations. Wherein, in the example 330-338, the weight percentages of valine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 339-
Example 339-347 provides a control agent for rice diseases, which is prepared by compounding 15% validamycin (OD)33.3 g/acre with valine at different concentrations. In example 339-347, the weight percentages of valine are 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 348-
Example 348-. In example 348-356, the weight percentages of valine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 357-
Example 357-365 provides a control agent for rice diseases, which is prepared by compounding 25% propiconazole (EC)60 mL/acre with valine at different concentrations. In example 357-365, the weight percentages of valine were 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively.
Example 366-
Example 366-. In example 366-.
Comparative example 1
The present example provides a control example of clear water.
Test example 1 measurement of growth inhibitory Effect of Ustilaginoidea virens on Rice
1. Growth inhibitory effect of L-leucine on Ustilaginoidea virens
1.1 culture of Ustilaginoidea virens
The rice false smut is cultured in a constant temperature incubator at 25 ℃, and the selected culture medium is a potato agar solid (PDA) culture medium.
1.2 inoculation of Rice pathogens
Burning the inoculating needle to red with alcohol lamp in a clean bench, cooling, cutting the mycelia with the inoculating needle from the edge of the mycelia together with the culture medium, vertically cutting the mycelia with a size of 1cm × 1cm, picking the mycelia with the inoculating needle to the middle position of a new solid culture medium, and sealing with a sealing film for culturing.
1.3 determination of growth inhibitory Effect of Ustilaginoidea virens on Rice
Taking eight erlenmeyer flasks, preparing seven L-leucine solutions with different concentrations of 30mL, 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4% in seven of the eight erlenmeyer flasks according to the concentrations of the L-leucine provided in the embodiments 1-7, wherein the eighth erlenmeyer flask is a blank control group, adding 1.3g of PDA culture medium into each erlenmeyer flask, sealing the openings of the erlenmeyer flasks with tinfoil paper and rubber bands, placing the erlenmeyer flasks in a high-temperature sterilization pot for sterilization, pouring the sterilized culture medium into disposable culture dishes in a clean bench in time when the erlenmeyer flasks are not completely cooled, pouring 10 mL of culture medium into each culture dish, pouring three dishes in each group, cooling, solidifying, sealing and inverting to obtain the solid culture medium.
Inverting the solid culture medium for three days, determining that the solid culture medium can be used for inoculating ustilaginoidea virens after no microbial contamination, inoculating a bacterium block into each culture dish by using an inoculating needle sterilized by an alcohol lamp at high temperature, treating eight groups in total, repeating three treatment groups in each group, sealing by using a sealing film, culturing in an incubator at 25 ℃, measuring the growth diameter of ustilaginoidea virens in each group after 21 days, and calculating the inhibition rate of each group on the growth of the ustilaginoidea virens according to the measurement result of a control group.
From the results of the inhibition rates of each group, a line graph of the growth inhibitory effect of L-leucine on Ustilaginoidea virens was obtained, and as shown in FIG. 1, L-leucine was found to have a certain inhibitory effect on the growth of Ustilaginoidea virens at concentrations of 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4% as compared with the blank control, and the greater the concentration of L-leucine, the more significant the inhibitory effect.
2. Growth inhibitory effect of L-isoleucine on Ustilaginoidea virens
The same method as that for the test of the growth inhibition of Ustilaginoidea virens by L-leucine to the growth of Ustilaginoidea virens is adopted to test the inhibition effect of the L-isoleucine solution provided in the embodiment 8-17 to the growth of Ustilaginoidea virens, in which eleven groups were set in total according to the concentrations of L-isoleucine in examples 8-17, namely, the control group and the treatment groups having concentrations of L-isoleucine of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively, and the test results are shown in FIG. 2 and can be obtained from the graphs, compared with a blank control, L-isoleucine concentrations of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32% all have certain inhibition effects on the growth of ustilaginoidea virens, and the greater the concentration of L-isoleucine, the more significant the inhibition effect.
3. Growth inhibitory effect of L-isoleucine on Ustilaginoidea virens
The same test method as that for the growth inhibition of Ustilaginoidea virens by L-leucine was used to determine the inhibition effect of the valine solution provided in examples 18-26 on the growth of Ustilaginoidea virens, wherein ten groups were determined according to the valine concentration in examples 18-26, namely, the control group and the treatment group having valine concentrations of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%, respectively, and the test results are shown in FIG. 3, and it can be seen from the figure that, compared with the blank control, valine concentrations of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16% all have a certain inhibition effect on the growth of Ustilaginoidea virens, and the greater the L-isoleucine concentration, the more significant the inhibition effect.
4. Growth inhibition effect of branched chain amino acid compound on ustilaginoidea virens
The same method as the L-leucine test method for the growth inhibition of Ustilaginoidea virens on rice is adopted to determine the inhibition effect of the branched chain amino acid compound solution provided in the embodiment 366-377 on the growth of Ustilaginoidea virens, wherein 13 groups are set according to the branched chain amino acid compound concentration in the embodiment 366-377, the compound concentrations of the control group and the branched chain amino acid are respectively 0.4 percent of L-leucine and 0.5 percent of L-isoleucine, 0.4 percent of L-leucine and 4 percent of L-isoleucine, 1.6 percent of L-leucine and 0.5 percent of L-isoleucine, 1.6 percent of L-leucine and 4 percent of L-isoleucine, 0.4 percent of L-leucine and 0.5 percent of valine, 0.4 percent of L-leucine and 4 percent of valine, 1.6 percent of L-leucine and 1.6 percent of valine and 4 percent of L-isoleucine, 0.5 percent of L-isoleucine and 0.5 percent of valine, 0.5 percent of L-isoleucine and 4 percent of valine, 1.6 percent of L-isoleucine and 0.5 percent of valine and 1.6 percent of L-isoleucine and 4 percent of valine, and the test result is shown in figure 4, the test result can be obtained from the figure, compared with a blank control, the branched chain amino acid compound solution has a certain inhibition effect on the growth of ustilaginoidea virens, and has a better inhibition effect than that of singly using the amino acid with the same concentration, wherein the inhibition effect of the 1.6 percent L-leucine compound solution is most obvious.
Test example 2 determination of influence of disease amount of false smut of rice of various rice varieties
1. Influence of L-leucine on the number of false rice grains of different rice varieties
1.1 Rice planting
Soaking rice seeds in 25% NaCl, stirring for 1-2min, standing, and selecting seeds with sunk bottoms and full seeds for planting; wrapping the seeds with sterilized gauze, and soaking in 70% ethanol for 1 min; soaking seeds for 15 min in 500mL of 2.5% sodium hypochlorite solution and 1 drop of Tween 80; washing the seeds with sterilized water in a super clean bench for 5 times, and absorbing excessive water with sterilized paper; taking out the seeds, placing the seeds in a sterilized culture dish, soaking the seeds in ultrapure water, and placing the seeds in an incubator at 25 ℃ for pregermination for 1 d; keeping the culture dish in a wet state, ensuring that the water surface can not submerge the seeds, and transferring the seedlings to a rice culture bottle containing an MS solid culture medium for culture at 25 ℃ after the seeds grow germs; and after 7 days of culture, transferring the seedlings into indoor shallow soil for culture, after 30 days, transferring the seedlings into an outdoor rice culture barrel for culture, and planting 5 seedlings in each barrel for inoculation.
1.2 preparation and inoculation of Ustilaginoidea virens inoculum
Inoculating the ustilaginoidea virens blocks into a PS culture solution by using an inoculating needle in a super-clean workbench, and carrying out shaking culture at the temperature of 27 ℃ and at the speed of 150r/min for 7 days to obtain a large amount of hypha and thin-walled conidia; and (3) smashing hyphae in the bacterial liquid by using a tissue blender to form a mixed liquid of hyphae fragments and the parenchyma conidia, and diluting the mixed liquid by 2 times by using potato cooking juice.
Sucking 2mL of inoculation liquid by using a sterile syringe, and injecting the inoculation liquid into the rice ear bags 6-9 d before the cutting and heading from the side until the inoculation liquid is full and overflows, wherein the inoculation is most easy to be carried out in the evening.
1.3L-leucine treatment of inoculated ears
After the rice seedlings were inoculated and the ears were spiked, seven L-leucine solutions of different concentrations, 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4%, were sprayed on the ears with sterilized ultrapure water in accordance with the concentrations of L-leucine provided in examples 1 to 7, 5 inoculated ears were sprayed on each group with sterilized ultrapure water as a control, the application was carried out 3 times in total (application interval 7d), the incidence difference of false smut between the treatments was observed and the number of curly rice balls per ear was recorded. As shown in FIG. 5, L-leucine has a certain effect on the number of rice koji balls of different rice varieties, and the larger the concentration of L-leucine, the smaller the number of rice koji balls, i.e., the more significant the effect of inhibiting the incidence of Ustilaginoidea virens.
2. Influence of L-isoleucine on number of rice koji balls of different rice varieties
The influence of the L-isoleucine solution provided in examples 8 to 17 on the number of rice koji balls of different rice varieties was determined by the same method as that of L-leucine on the number of rice koji balls of different rice varieties, wherein eleven groups were set in total according to the L-isoleucine concentrations in examples 8 to 17, and the test results were shown in FIG. 6, in which L-isoleucine had a certain influence on the number of rice koji balls of different rice varieties and the treatment groups had L-isoleucine concentrations of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%, respectively, and the larger the L-isoleucine concentration was, the smaller the number of rice koji balls was, i.e., the more significant the effect of suppressing the incidence of Ustilaginella virescens.
3. Effect of valine on the number of koji balls of different rice varieties
The effect of the valine solution provided in examples 18 to 26 on the number of koji balls of different rice varieties was determined in the same manner as the effect of L-leucine on the number of koji balls of different rice varieties, wherein ten groups were determined based on the valine concentrations in examples 18 to 26, wherein the test results were shown in FIG. 7, in which valine had a certain effect on the number of koji balls of different rice varieties, and the larger the valine concentration was, the smaller the number of koji balls was, i.e., the more significant the effect of inhibiting the incidence of Ustilaginella virescens was, in comparison with the control group and the treatment groups having valine concentrations of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Test example 3 determination of Effect of branched-chain amino acid-based auxiliary Agents on prevention of false smut
1. Determination of prevention effect of L-leucine auxiliary agent on false smut
The test was conducted in a large area, the test site was in the Yuanqian town of the Xiaoshan district, the rice variety to be tested was Yongyou 538, and 75% Oxidomycete-tebuconazole (WDG) (15 g/mu), 43% tebuconazole (SC) (15 g/mu), 30% hexaconazole (SC) (22.5 g/mu), 15% benzyl propiconazole (SC) (30 g/mu), 32.5% benzyl azoxystrobin (SC) (60 g/mu), 61% validamycin (SP) (8.196 g/mu), 12.5% diniconazole (WP) (45 g/mu), 10% difenoconazole (WP) (30 g/mu), 15% triazolone (WP) (100 g/mu), 21.5% tebuconazole (OD) (30 g/mu), 15% validamycin (OD) (33.3 g/mu), 12% thifluzamide (OD) (46 g/mu), and, The method comprises the steps of compounding 25% propiconazole (EC) (60 mL/mu) and blank with seven L-leucine with different concentrations of 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4% respectively and using clear water as a reference, setting up 112 groups for treatment, carrying out first pesticide application 10 days before the rice is cut open, carrying out second pesticide application 5 days before the rice is cut open, and carrying out conventional spraying by using an electric sprayer. Each group in the stage of wax ripening randomly investigated 20 clusters to investigate the incidence of false smut.
Classification standard of false smut: level 0: does not occur; level 1: 1 particle of each ear; and 3, level: 2-4 diseased grains per ear; and 5, stage: 5-8 diseased grains per ear; and 7, stage: 9-15 diseased grains per ear; and 9, stage: more than 16 diseased grains per ear.
Note: the yellow world of the rice institute of China.
The relative control effect is (disease finger in control area-disease finger in control area)/disease finger in control area x 100%
The control effect is shown in table 1: wherein the percentage of the effect of prevention is shown in the table, it can be seen that the application of L-leucine together with the chemical pesticides mentioned in examples 27 to 118 can improve the effect of the pesticide on false smut and improve the effect of preventing false smut, and the higher the concentration is, the better the effect is.
TABLE 1 prevention of Ustilaginoidea virens by L-leucine in combination with chemical pesticides
2. Determination of prevention effect of L-isoleucine auxiliary agent on false smut
By adopting the method for determining the false smut prevention effect with the L-leucine auxiliary agent and the prevention effect calculation method, according to the comparison between the agent provided in example 119-charge 248 and the L-isoleucine with the concentration of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32% and the clear water, 154 groups of treatment are provided in total to obtain the result of the L-isoleucine matched agent for preventing false smut, as shown in Table 2, wherein the percentage of the prevention effect is shown in the table, and can be obtained from the table, when the L-isoleucine is applied together with the chemical pesticide mentioned in example 119-charge 248, the prevention effect of the agent on false smut can be improved, and the prevention effect of false smut is improved, and the prevention effect is better as the concentration is higher.
TABLE 2 prevention of Ustilaginoidea virens by L-isoleucine in combination with chemical pesticides
3. Determination of prevention effect of valine auxiliary agent on false smut
By adopting the method for determining the false smut prevention effect of the L-leucine auxiliary agent and the prevention effect calculation method, 140 groups of treatment are set in total according to the comparison of the agent provided in example 249-365 with valine with concentrations of 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16% and clear water, and the result of the false smut prevention effect of the valine matched with the agent is obtained, as shown in Table 3, wherein the percentage of the prevention effect is shown, and the result can be obtained from the table that the application of the valine and the chemical pesticide provided in example 249-365 together can improve the false smut prevention effect of the agent and the false smut prevention effect, and the greater the concentration is, the better the prevention effect is.
TABLE 3 preventive effect of valine in combination with chemical pesticides on Ustilaginoidea virens
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. The application of branched chain amino acid in preparing a medicament for preventing and treating rice diseases is characterized in that the rice diseases are false smut, and the branched chain amino acid comprises a mixture of any two of L-leucine, L-isoleucine and valine.
2. The pesticide for preventing and treating the rice diseases is characterized by comprising branched chain amino acid and a carrier, wherein the rice diseases are false smut, and the branched chain amino acid comprises a mixture of any two of L-leucine, L-isoleucine and valine;
when the preventing and treating agent for the rice diseases contains the L-leucine, the L-leucine accounts for 0.05-2.4% of the preventing and treating agent for the rice diseases by weight percent;
when the preventing and treating agent for rice diseases contains the L-isoleucine, the L-isoleucine accounts for 0.05-32% of the preventing and treating agent for rice diseases by weight percent;
when the rice disease control agent contains the valine, the valine accounts for 0.05-16% of the weight of the rice disease control agent.
3. The agent for controlling rice diseases according to claim 2, wherein the carrier comprises one or more of water, glucose, lactose, gum arabic, gelatin, mannitol, starch, magnesium trisilicate, talc, corn starch, keratin, colloidal silicon dioxide, potato starch, sandy soil, and nano titanium dioxide.
4. The agent for controlling rice diseases according to claim 2, characterized by further comprising an auxiliary material selected from one or more of a stabilizer, a thickener, a colorant and a perfume.
5. The agent for controlling a rice disease according to claim 2, characterized by further comprising a pesticide including one or more of oximidofen-tebuconazole, hexaconazole, benzyl propiconazole, azoxystrobin, validamycin, diniconazole, difenoconazole, triazolone, thifluzamide, and propiconazole.
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