CN109832273A - The pesticide control of application and rice disease of the branched-chain amino acid in the pesticide control of preparation rice disease - Google Patents
The pesticide control of application and rice disease of the branched-chain amino acid in the pesticide control of preparation rice disease Download PDFInfo
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Abstract
The present invention relates to compound technicals, in particular to the pesticide control of application and rice disease of the branched-chain amino acid in the pesticide control of preparation rice disease, the pesticide control of the rice disease comprising branched-chain amino acid and carrier.Branched-chain amino acid of the invention can effectively inhibit infecting for ustilaginoidea virens, the formation of rice curve and the generation of rice song toxin, reduce rice green smut disease incidence, to which the growth of rice be effectively ensured, the formation of spike of rice, and the generation of toxin is reduced, improve rice yield and quality.The pesticide control of rice disease provided by the invention is using branched-chain amino acid provided by the invention as the effective component of prevention and treatment Ustilaginoidea virens, the invasion of ustilaginoidea virens can effectively be inhibited, growth, breeding and the generation of toxin, reduce the disease incidence of rice green smut, to effectively guarantee the growth of rice and the formation of spike of rice, and the generation of rice song toxin is reduced, improves the yield and quality of rice.
Description
Technical field
The present invention relates to compound technicals, in particular to branched-chain amino acid in the prevention and treatment for preparing rice disease
The pesticide control of application and rice disease in medicament.
Background technique
Rice green smut is a kind of fungal disease in rice earing stage morbidity, shows as being formed in Rice Panicle greater than rice
The blackish green rice curve of grain, is the rice disease that disease incidence significantly increases in recent years, since this disease once infects morbidity just
It is difficult to prevent and treat, not only causes the underproduction of rice, can also generate rice song toxin, people and livestock will appear in viscera etc. after eating by mistake
Malicious phenomenon.
Therefore, those skilled in the art need to develop a kind of novel rice false smut pesticide control, to inhibit rice rice bent
The increment of germ reduces the disease incidence of false smut, guarantees rice healthy growth, reduces farmland endotoxin contamination, improves food safety.
Summary of the invention
The purpose of the present invention is to provide application of the branched-chain amino acid in the pesticide control of preparation rice disease, with effective
Ground inhibits the growth of Ustilaginoidea virens and the generation of toxin, reduces the disease incidence of rice green smut, guarantees rice normal growth,
To improve the yield and quality of rice.
Another object of the present invention is to provide the pesticide control of rice disease, can inhibit the growth of Ustilaginoidea virens with
The generation of toxin reduces the disease incidence of rice green smut, effectively reduces field endotoxin contamination, guarantees rice normal growth, thus
The yield and quality of rice is improved, guarantees food safety.
The embodiment of the present invention is achieved in that
Application of the branched-chain amino acid in the pesticide control of preparation rice disease.
The pesticide control of rice disease comprising branched-chain amino acid and carrier.
The beneficial effect of the embodiment of the present invention includes:
Application of the branched-chain amino acid provided by the invention in the pesticide control of preparation rice disease, branched-amino therein
Acid can effectively inhibit infecting for ustilaginoidea virens, the formation of rice curve and the generation of rice song toxin, reduce rice green smut morbidity
Rate, so that the growth of rice is effectively ensured, the formation of spike of rice, and the generation of toxin is reduced, improve rice yield and quality.This hair
The pesticide control of the rice disease of bright offer is using branched-chain amino acid provided by the invention as the effective of prevention and treatment Ustilaginoidea virens
Ingredient can effectively inhibit the invasion of ustilaginoidea virens, growth, and breeding and the generation of toxin reduce the disease incidence of rice green smut,
To effectively guarantee the growth of rice and the formation of spike of rice, and the generation of rice song toxin is reduced, improves the yield and product of rice
Matter.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is L-Leu to ustilaginoidea virens growth inhibitory effect line chart；
Fig. 2 is l-Isoleucine to ustilaginoidea virens growth inhibitory effect line chart；
Fig. 3 is valine to ustilaginoidea virens growth inhibitory effect line chart；
Fig. 4 is that branched-chain amino acid is compounded to ustilaginoidea virens growth inhibitory effect column diagram；
Fig. 5 is that L-Leu influences column diagram to different rice varieties rice curve quantity；
Fig. 6 is that l-Isoleucine influences column diagram to different rice varieties rice curve quantity；
Fig. 7 is that valine influences column diagram to different rice varieties rice curve quantity.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Application to the branched-chain amino acid of the embodiment of the present invention in the pesticide control of preparation rice disease and water below
The pesticide control of rice disease is specifically described.
False smut is a kind of rice earing stage fungal disease as caused by rice green pyrenomycetes, at home the Yangtze river basin, Hebei and south
Square each province paddy fields happen occasionally, also known as pseudo- smut, and green smut is commonly called as " high yield fruit ".When Rice Heading is bloomed,
Pathogen invades floral organ and children's grain husk, parasitizes in rice glume, the flourish in glume, and expand grain and split, rear to wrap
Full glume is wrapped up in, forms 3-4 times of rice curve infected seed bigger than healthy grain, rice curve prior surface is smooth, and the later period, which splits, to give out
Blackish green chlamydospore, or the flat sclerotium of black is generated, it being fallen into soil through exposing to wind and rain, next year, which sprouts, generates chlamydospore,
Chlamydospore generates microspore and ascospore carries out First aggression.In Rice Heading blooming stage, be conducive to the generation of false smut.It takes out
The kind morbidity of fringe morning is lighter, and hybridization high-yield rice morbidity is heavier, and the generation of false smut in addition can be also aggravated using excessive nitrogenous fertilizer.
False smut not only will affect the yield of producing region rice, increase abortive grain rate, green rice-grain rate, broken rice rate, and the local field underproduction 20%~
30%, the mycotoxin for having severe toxicity to act on people and livestock can be generated, and can cause when containing 0.5% infected seed in paddy
People, poultry poisoning.
Due to this disease once occurring just to be difficult to reverse, currently, in terms of ustilaginoidea virens prevention and treatment, with " comprehensive anti-based on prevention and control
Control " plant protection policy be guidance, comprehensive measures for the prevention and control taken to easily susceptible hybrid rice and disease-resistant japonica rice, reinforces cultivating
Management, sprays triazolone, the medicaments such as carbendazim or jinggangmeisu can prevent the morbidity of false smut, loss is dropped to most before cut
Report that is low, but there is no branched-chain amino acid to apply in rice green smut prevention and treatment so far.
In the present embodiment, application of the branched-chain amino acid in the pesticide control of preparation rice disease is proposed, by that will prop up
Pesticide control of the chain amino acid as main active preparation rice disease, to effectively inhibit the growth of Ustilaginoidea virens
With the generation of toxin, the disease incidence of rice green smut is reduced, guarantees rice normal growth, to improve the yield and product of rice
Matter.
Wherein, rice disease includes but is not limited to rice fungus diseases evil, rice bacteriosis and Rice Virus disease.
Wherein, rice fungus diseases evil includes but is not limited to rice blast, banded sclerotial blight, flax leaf spot, culm rot, dislikes seedling
Disease, downy mildew, false smut and sheath rot disease etc..Rice bacteriosis includes but is not limited to bacterial blight of rice, bacillary
Cecospora spot and bacterial Footrot etc..Rice Virus disease includes but is not limited to stripe virus disease and black streak dwarf etc..
Through inventor the study found that the prevention and treatment for the rice disease that branched-chain amino acid is prepared as main active
Medicament can effectively inhibit rice disease, especially obvious to the inhibitory effect of the false smut in rice fungus diseases evil, can be effective
Inhibit the disease incidence of rice green smut, so that the growth of rice be effectively ensured, improves yield and quality of rice.It should be understood that this reality
The pesticide control for applying the rice disease provided in example can be used for inhibiting other kinds of rice disease.
In the present embodiment, branched-chain amino acid includes one of L-Leu, l-Isoleucine and valine or a variety of.
Wherein, L-Leu has the following structure formula:
L-Isoleucine has the following structure formula:
Valine has the following structure formula:
In addition, present invention also provides the pesticide controls of rice disease comprising branched-chain amino acid and carrier.
In the pesticide control of rice disease provided by the invention, branched-chain amino acid can be any form, such as solid, half
The forms such as solid, liquid or aerosol.
Wherein, it is 0.05%-32% that branched-chain amino acid, which accounts for the weight percent of the pesticide control of rice disease,.
Just as described above, branched-chain amino acid includes one of L-Leu, l-Isoleucine and valine or a variety of.
Wherein, when in the pesticide control of rice disease including L-Leu, L-Leu accounts for the pesticide control of the rice disease
Weight percent is 0.05%-2.4%；When in the pesticide control of rice disease including l-Isoleucine, l-Isoleucine is accounted for
The weight percent of the pesticide control of the rice disease is 0.05%-32%；When in the pesticide control of rice disease include figured silk fabrics
When propylhomoserin, the weight percent that valine accounts for the pesticide control of the rice disease is 0.05%-16%.
In typical but non-limiting embodiment of the invention, in rice green smut pesticide control, L-Leu contains
Amount be, for example, any one of 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% or 2.4% or it is any between the two
Value range, l-Isoleucine content be, for example, 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% or
Any one of 32% or any value range between the two, valine content is, for example, 0.05%, 0.1%, 0.25%,
0.5%, any one of 1%, 2%, 4%, 8% or 16% or any value range between the two, rice rice can be reduced
The disease incidence of bent disease, branched-amino acid concentration is higher, and control efficiency is better.
In typical but non-limiting embodiment of the invention, in rice green smut pesticide control, by L-Leu,
Any two kinds of mixture in l-Isoleucine and valine is made, for example, bright containing L-Leu and l-Isoleucine, L-
Propylhomoserin and valine or l-Isoleucine and valine.
Further, in above-mentioned compound formulation, the content of L-Leu is 0.05%-2.4%, preferably 0.4%-
1.6%, the content of l-Isoleucine is 0.05%-32%, and preferably 0.5-4%, the content of valine is 0.05%-16%,
Preferably 0.5%-4%.It specifically, may include: L-Leu 0.4% and l-Isoleucine 0.5% in above-mentioned compound formulation,
L-Leu 0.4% and l-Isoleucine 4%, L-Leu 1.6% and l-Isoleucine 0.5%, L-Leu 1.6% and L-
Isoleucine 4%, L-Leu 0.4% and valine 0.5%, L-Leu 0.4% and valine 4%, L-Leu 1.6%
With valine 0.5%, L-Leu 1.6% and valine 4%, l-Isoleucine 0.5% and valine 0.5%, the different bright ammonia of L-
Acid 0.5% and valine 4%, l-Isoleucine 1.6% and valine 0.5%, l-Isoleucine 1.6% and valine 4%,
The disease incidence of rice green smut can be reduced.
In typical but non-limiting embodiment of the invention, rice green smut pesticide control can also be by the bright ammonia of L-
Acid, l-Isoleucine and valine this three mixture be made.
The pesticide control of rice disease provided by the present application can also include the carrier of branched-chain amino acid.For example including but not
Be limited to water, glucose, lactose, Arabic gum, gelatin, mannitol, starch, magnesium trisilicate, talcum, cornstarch, keratin,
One of colloidal silicon dioxide, potato starch, sandy soil and nano-titanium dioxide are a variety of, also may include solid in preparation
Body, semisolid, liquid or aerosol form preparation used in other carriers.
Optionally, the pesticide control of rice disease further includes auxiliary material, auxiliary material include but is not limited to stabilizer, thickener,
One of toner and fragrance are a variety of.
Optionally, the pesticide control of rice disease further includes pesticide, and pesticide includes but is not limited to oxime bacterium Tebuconazole, penta azoles
Alcohol, azoles alcohol, benzene first propiconazole, benzene first Fluoxastrobin, jinggangmeisu, olefin conversion, difenoconazole, triazolone, Tebuconazole,
One of jinggangmeisu, thifluzamide and propiconazole are a variety of.
The present invention also provides the application methods of the pesticide control of rice disease, i.e., spray the pesticide control of rice disease
In on rice spike of rice.
Rice green smut pesticide control provided by the invention is using branched-chain amino acid provided by the invention as prevention and treatment rice rice
The effective component of bent germ can effectively inhibit the invasion of ustilaginoidea virens, growth, and breeding and the generation of toxin reduce rice rice
The disease incidence of bent disease, to effectively guarantee the growth of rice and the formation of spike of rice, and reduces the generation of rice song toxin, improves water
The yield and quality of rice.
With reference to embodiments to branched-chain amino acid of the invention preparation rice disease pesticide control in application with
And the pesticide control further progress of rice disease illustrates.
Embodiment 1-7
Embodiment 1-7 provides a kind of pesticide control of rice disease, and main component is L- leucine, and water is solvent.
The concentration of L-Leu is formulated as 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and respectively in embodiment 1-7
2.4%.
Embodiment 8-17
Embodiment 8-17 provides a kind of pesticide control of rice disease, and main component is L- isoleucine, and water is molten
Agent.In embodiment 8-17 the concentration of l-Isoleucine be formulated as 0.05% respectively, 0.1%, 0.25%, 0.5%, 1%, 2%,
4%, 8%, 16% and 32%.
Embodiment 18-26
Embodiment 18-26 provides a kind of pesticide control of rice disease, and main component is valine, and water is solvent.
The concentration of valine is formulated as 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% respectively in embodiment 18-26
With 16%.
Embodiment 27-34
Embodiment 27-34 provides a kind of pesticide control of rice disease, is by 75% oxime bacterium Tebuconazole (WDG)
15g/ mus are compounded with the L-Leu of various concentration and are obtained.Wherein, in embodiment 27-34, the weight percent of L-Leu
Than being respectively 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 35-41
Embodiment 35-41 provides a kind of pesticide control of rice disease, be by 15g/ mus of 43% Tebuconazole (SC) with
The L-Leu of various concentration is compounded and is obtained.Wherein, in embodiment 35-41, the weight percent of L-Leu is respectively
0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 42-48
Embodiment 42-48 provides a kind of pesticide control of rice disease, is by 30% 22.5g/ mus of azoles alcohol (SC)
It is compounded and is obtained with the L-Leu of various concentration.Wherein, in embodiment 42-48, the weight percent of L-Leu is distinguished
It is 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 49-55
Embodiment 49-55 provides a kind of pesticide control of rice disease, is by 15% benzene first propiconazole (SC)
30g/ mus are compounded with the L-Leu of various concentration and are obtained.Wherein, in embodiment 49-55, the weight percent of L-Leu
Than being respectively 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 56-62
Embodiment 56-62 provides a kind of pesticide control of rice disease, is by 32.5% benzene first Fluoxastrobin (SC)
60g/ mus are compounded with the L-Leu of various concentration and are obtained.Wherein, in embodiment 56-62, the weight percent of L-Leu
Than being respectively 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 63-69
Embodiment 63-69 provides a kind of pesticide control of rice disease, is by 61% jinggangmeisu (SP) 8.196g/
Mu is compounded with the L-Leu of various concentration and is obtained.Wherein, in embodiment 63-69, the weight percent score of L-Leu
It Wei 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 70-76
Embodiment 70-76 provides a kind of pesticide control of rice disease, is by 45g/ mus of 12.5% olefin conversion (WP)
It is compounded and is obtained with the L-Leu of various concentration.Wherein, in embodiment 70-76, the weight percent of L-Leu is distinguished
It is 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 77-83
Embodiment 77-83 provides a kind of pesticide control of rice disease, is by 10% difenoconazole (WP) 30g/
Mu is compounded with the L-Leu of various concentration and is obtained.Wherein, in embodiment 77-83, the weight percent score of L-Leu
It Wei 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 84-90
Embodiment 84-90 provides a kind of pesticide control of rice disease, be by 100g/ mus of 15% triazolone (WP) with
The L-Leu of various concentration is compounded and is obtained.Wherein, in embodiment 84-90, the weight percent of L-Leu is respectively
0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 91-97
Embodiment 91-97 provides a kind of pesticide control of rice disease, is by 30g/ mus of 21.5% Tebuconazole (OD)
It is compounded and is obtained with the L-Leu of various concentration.Wherein, in embodiment 91-97, the weight percent of L-Leu is distinguished
It is 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 98-104
Embodiment 98-104 provides a kind of pesticide control of rice disease, is by 15% jinggangmeisu (OD) 33.3g/
Mu is compounded with the L-Leu of various concentration and is obtained.Wherein, in embodiment 98-104, the weight percent score of L-Leu
It Wei 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 105-111
Embodiment 105-111 provides a kind of pesticide control of rice disease, is by 12% thifluzamide (OD) 46g/
Mu is compounded with the L-Leu of various concentration and is obtained.Wherein, in embodiment 105-111, the weight percent of L-Leu
Respectively 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 112-118
Embodiment 112-118 provides a kind of pesticide control of rice disease, is by 60mL/ mus of 25% propiconazole (EC)
It is compounded and is obtained with the L-Leu of various concentration.Wherein, in embodiment 112-118, the weight percent score of L-Leu
It Wei 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 2.4%.
Embodiment 119-128
Embodiment 119-128 provides a kind of pesticide control of rice disease, is by 75% oxime bacterium Tebuconazole (WDG)
15g/ mus are compounded with the l-Isoleucine of various concentration and are obtained.Wherein, in embodiment 119-128, the weight of l-Isoleucine
Measuring percentage is respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 129-138
Embodiment 129-138 provides a kind of pesticide control of rice disease, is by 15g/ mus of 43% Tebuconazole (SC)
It is compounded and is obtained with the l-Isoleucine of various concentration.Wherein, in embodiment 129-138, the weight percent of l-Isoleucine
Than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 139-148
Embodiment 139-148 provides a kind of pesticide control of rice disease, is by 30% azoles alcohol (SC) 22.5g/
Mu is compounded with the l-Isoleucine of various concentration and is obtained.Wherein, in embodiment 139-148, the weight hundred of l-Isoleucine
Divide than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 149-158
Embodiment 149-158 provides a kind of pesticide control of rice disease, is by 15% benzene first propiconazole (SC)
30g/ mus are compounded with the l-Isoleucine of various concentration and are obtained.Wherein, in embodiment 149-158, the weight of l-Isoleucine
Measuring percentage is respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 159-168
Embodiment 159-168 provides a kind of pesticide control of rice disease, is by 32.5% benzene first Fluoxastrobin
(SC) it is compounded and is obtained with the l-Isoleucine of various concentration for 60g/ mus.Wherein, in embodiment 159-168, l-Isoleucine
Weight percent be respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 169-178
Embodiment 169-178 provides a kind of pesticide control of rice disease, is by 61% jinggangmeisu (SP)
8.196g/ mus are compounded with the l-Isoleucine of various concentration and are obtained.Wherein, in embodiment 169-178, l-Isoleucine
Weight percent is respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 179-188
Embodiment 179-188 provides a kind of pesticide control of rice disease, is by 12.5% olefin conversion (WP) 45g/
Mu is compounded with the l-Isoleucine of various concentration and is obtained.Wherein, in embodiment 179-188, the weight hundred of l-Isoleucine
Divide than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 189-198
Embodiment 189-198 provides a kind of pesticide control of rice disease, is by 10% difenoconazole (WP)
30g/ mus are compounded with the l-Isoleucine of various concentration and are obtained.Wherein, in embodiment 189-198, the weight of l-Isoleucine
Measuring percentage is respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 199-208
Embodiment 199-208 provides a kind of pesticide control of rice disease, is by 100g/ mus of 15% triazolone (WP)
It is compounded and is obtained with the l-Isoleucine of various concentration.Wherein, in embodiment 199-208, the weight percent of l-Isoleucine
Than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 209-218
Embodiment 209-218 provides a kind of pesticide control of rice disease, is by 21.5% Tebuconazole (OD) 30g/
Mu is compounded with the l-Isoleucine of various concentration and is obtained.Wherein, in embodiment 209-218, the weight hundred of l-Isoleucine
Divide than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 219-228
Embodiment 219-228 provides a kind of pesticide control of rice disease, is by 15% jinggangmeisu (OD)
33.3g/ mus are compounded with the l-Isoleucine of various concentration and are obtained.Wherein, in embodiment 219-228, l-Isoleucine
Weight percent is respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 229-238
Embodiment 229-238 provides a kind of pesticide control of rice disease, is by 12% thifluzamide (OD) 46g/
Mu is compounded with the l-Isoleucine of various concentration and is obtained.Wherein, in embodiment 229-238, the weight hundred of l-Isoleucine
Divide than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 239-248
Embodiment 239-248 provides a kind of pesticide control of rice disease, is by 60mL/ mus of 25% propiconazole (EC)
It is compounded and is obtained with the l-Isoleucine of various concentration.Wherein, in embodiment 239-248, the weight percent of l-Isoleucine
Than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32%.
Embodiment 249-257
Embodiment 249-257 provides a kind of pesticide control of rice disease, is by 75% oxime bacterium Tebuconazole (WDG)
15g/ mus are compounded with the valine of various concentration and are obtained.Wherein, in embodiment 249-257, the weight percent of valine
Respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 258-266
Embodiment 258-266 provides a kind of pesticide control of rice disease, is by 15g/ mus of 43% Tebuconazole (SC)
It is compounded and is obtained with the valine of various concentration.Wherein, in embodiment 258-266, the weight percent of valine is respectively
0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 267-275
Embodiment 267-275 provides a kind of pesticide control of rice disease, is by 30% azoles alcohol (SC) 22.5g/
Mu is compounded with the valine of various concentration and is obtained.Wherein, in embodiment 267-275, the weight percent of valine is distinguished
It is 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 276-284
Embodiment 276-284 provides a kind of pesticide control of rice disease, is by 15% benzene first propiconazole (SC)
30g/ mus are compounded with the valine of various concentration and are obtained.Wherein, in embodiment 276-284, the weight percent of valine
Respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 285-293
Embodiment 285-293 provides a kind of pesticide control of rice disease, is by 32.5% benzene first Fluoxastrobin
(SC) it is compounded and is obtained with the valine of various concentration for 60g/ mus.Wherein, in embodiment 285-293, the weight hundred of valine
Divide than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 294-302
Embodiment 294-302 provides a kind of pesticide control of rice disease, is by 61% jinggangmeisu (SP)
8.196g/ mus are compounded with the valine of various concentration and are obtained.Wherein, in embodiment 294-302, the weight percent of valine
Than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 303-311
Embodiment 303-311 provides a kind of pesticide control of rice disease, is by 12.5% olefin conversion (WP) 45g/
Mu is compounded with the valine of various concentration and is obtained.Wherein, in embodiment 303-311, the weight percent of valine is distinguished
It is 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 312-320
Embodiment 312-320 provides a kind of pesticide control of rice disease, is by 10% difenoconazole (WP)
30g/ mus are compounded with the valine of various concentration and are obtained.Wherein, in embodiment 312-320, the weight percent of valine
Respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 321-329
Embodiment 321-329 provides a kind of pesticide control of rice disease, is by 100g/ mus of 15% triazolone (WP)
It is compounded and is obtained with the valine of various concentration.Wherein, in embodiment 321-329, the weight percent of valine is respectively
0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 330-338
Embodiment 330-338 provides a kind of pesticide control of rice disease, is by 21.5% Tebuconazole (OD) 30g/
Mu is compounded with the valine of various concentration and is obtained.Wherein, in embodiment 330-338, the weight percent of valine is distinguished
It is 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 339-347
Embodiment 339-347 provides a kind of pesticide control of rice disease, is by 15% jinggangmeisu (OD)
33.3g/ mus are compounded with the valine of various concentration and are obtained.Wherein, in embodiment 339-347, the weight percent of valine
Than being respectively 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 348-356
Embodiment 348-356 provides a kind of pesticide control of rice disease, is by 12% thifluzamide (OD) 46g/
Mu is compounded with the valine of various concentration and is obtained.Wherein, in embodiment 348-356, the weight percent of valine is distinguished
It is 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 357-365
Embodiment 357-365 provides a kind of pesticide control of rice disease, is by 60mL/ mus of 25% propiconazole (EC)
It is compounded and is obtained with the valine of various concentration.Wherein, in embodiment 357-365, the weight percent of valine is respectively
0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%.
Embodiment 366-377
Embodiment 366-377 provides a kind of pesticide control of rice disease, and main component is L-Leu, and L- is different
Leucine, the mixture both in valine, water is solvent.In embodiment 366-377, branched-amino acid content is the bright ammonia of L-
Acid 0.4% and l-Isoleucine 0.5%, L-Leu 0.4% and l-Isoleucine 4%, L-Leu 1.6% and L- are different bright
Propylhomoserin 0.5%, L-Leu 1.6% and l-Isoleucine 4%, L-Leu 0.4% and valine 0.5%, L- leucine
0.4% with valine 4%, L-Leu 1.6% and valine 0.5%, L-Leu 1.6% and valine 4%, L- is different bright
Propylhomoserin 0.5% and valine 0.5%, l-Isoleucine 0.5% and valine 4%, l-Isoleucine 1.6% and valine
0.5%, l-Isoleucine 1.6% and valine 4%.
Comparative example 1
Present embodiments providing reference examples is clear water.
The measurement of 1 Ustilaginoidea virens growth inhibitory effect of test example
1, L-Leu is to ustilaginoidea virens growth inhibitory effect
The culture of 1.1 Ustilaginoidea virens
In 25 DEG C of constant incubators, the culture medium selected is potato agar solid (PDA) for Ustilaginoidea virens culture
Culture medium.
The inoculation of 1.2 rice pathogens
In superclean bench, transfer needle is burnt to aglow with alcolhol burner, it is cooling after with transfer needle from mycelium edge
Together with culture medium, the fungus block of 1cm × 1cm size is vertically cut, then is chosen fungus block into new solid medium with transfer needle
Between position, sealed membrane sealing culture.
The measurement of 1.3 Ustilaginoidea virens growth inhibitory effects
Eight conical flasks are taken, according to the concentration of the embodiment 1-7 L-Leu provided, are respectively matched in wherein seven conical flasks
The L-Leu solution of the various concentration of 30mL0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4% 7 processed,
Wherein the 8th conical flask is blank control group, 1.3g PDA culture medium is added in each conical flask, with masking foil and rubber
Conical flask bottleneck is tamping by muscle, is placed in high-temperature sterilization pot and is sterilized, in superclean bench in time will sterilizing when completely not cooling
Culture medium pour into batch cultur ware, each culture dish pours into 10 mL culture mediums, and every group is fallen three wares, seals after cooled and solidified
Mouth is inverted, and solid medium is obtained.
Solid medium is inverted three days, is determined without the inoculation that can be used for ustilaginoidea virens after microbial contamination, to alcolhol burner
Fungus block is inoculated in each culture dish by the transfer needle of high-temperature sterilization, totally eight groups of processing, and three repetitions of every group of processing are sealed with sealed membrane
In 25 DEG C of incubator cultures after mouthful, the growth diameter of ustilaginoidea virens in every group is measured after 21 days, according to the measurement knot of control group
Fruit calculates every group of inhibiting rate to ustilaginoidea virens growth.
According to every group of inhibiting rate as a result, obtaining L-Leu to ustilaginoidea virens growth inhibitory effect line chart, such as Fig. 1 institute
Show, it is available from figure, compared with blank control, 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4%
Concentration L-Leu has certain inhibitory effect to the growth of ustilaginoidea virens, and the concentration of L-Leu is bigger, inhibitory effect
It is more significant.
2, l-Isoleucine is to ustilaginoidea virens growth inhibitory effect
Embodiment 8-17 is measured to the identical method of Ustilaginoidea virens growth inhibition test method using with L-Leu
The depression effect that the l-Isoleucine solution of offer grows Ustilaginoidea virens, wherein according to the different bright ammonia of L- in embodiment 8-17
Acid concentration, sets 11 groups altogether, respectively control group and l-Isoleucine concentration be 0.05%, 0.1%, 0.25%, 0.5%,
1%, 2%, 4%, 8%, 16% and 32% processing group, test result is as shown in Fig. 2, available from figure, with blank pair
Photograph ratio, 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and 32% concentration l-Isoleucine pair
The growth of ustilaginoidea virens has certain inhibitory effect, and the concentration of l-Isoleucine is bigger, and inhibitory effect is more significant.
3, l-Isoleucine is to ustilaginoidea virens growth inhibitory effect
Embodiment 18-26 is measured to the identical method of Ustilaginoidea virens growth inhibition test method using with L-Leu
The depression effect that the valine solution of offer grows Ustilaginoidea virens, wherein according to valine concentration in embodiment 18-26,
Set ten groups altogether, respectively control group and valine concentration be 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%,
8% and 16% processing group, test result is as shown in figure 3, available from figure, compared with blank control, 0.05%,
0.1%, the valine of 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16% concentration has centainly the growth of ustilaginoidea virens
Inhibitory effect, and the concentration of l-Isoleucine is bigger, and inhibitory effect is more significant.
4, branched-chain amino acid compounding is to ustilaginoidea virens growth inhibitory effect
Embodiment 366- is measured to the identical method of Ustilaginoidea virens growth inhibition test method using with L-Leu
The depression effect that the 377 branched-chain amino acid combination solutions provided grow ustilaginoidea virens, wherein being propped up according in embodiment 366-377
Chain amino acid compounds concentration, sets 13 groups altogether, and it is L-Leu 0.4% that respectively control group, which compounds concentration with branched-chain amino acid,
With l-Isoleucine 0.5%, L-Leu 0.4% and l-Isoleucine 4%, L-Leu 1.6% and L- isoleucine
0.5%, L-Leu 1.6% and l-Isoleucine 4%, L-Leu 0.4% and valine 0.5%, L-Leu 0.4% with
Valine 4%, L-Leu 1.6% and valine 0.5%, L-Leu 1.6% and valine 4%, l-Isoleucine
0.5% with valine 0.5%, l-Isoleucine 0.5% and valine 4%, l-Isoleucine 1.6% and valine 0.5%,
L- isoleucine 1.6% and valine 4%, test result is as shown in figure 4, available from figure, compared with blank control,
Branched-chain amino acid combination solution has certain inhibitory effect, and the ammonia than concentration of the same race is used alone to the growth of ustilaginoidea virens
Base acid has better inhibitory effect, wherein most significant containing 1.6%L- leucine combination solution inhibitory effect.
The different rice varieties rice curve morbidity quantity of test example 2 influence measurement
1, L-Leu influences different rice varieties rice curve quantity
1.1 Rice Cropping
By rice paddy seed immerse 25%NaCl in, stir 1-2min after stand, selection sink to the bottom and the seed of full seed into
Row plantation；Seed is wrapped up with sterile gauze, impregnates 1min with 70% ethyl alcohol；Add 1 drop with 2.5% liquor natrii hypochloritis of 500mL
Tween 80 impregnates 15 min of seed；With sterilizing water washing seed 5 times in super-clean bench, extra moisture is sucked with sterilizing paper；It will
Seed taking-up is placed in sterilizes culture dish, and ultrapure water, which impregnates, does not cross seed, is placed in vernalization 1d in 25 DEG C of incubators；Make in culture dish
Moisture state is kept, but the water surface can not have seed, move to seedling containing MS solid medium after seed grows plumule
25 DEG C of cultures in rice culture bottle；Seedling is moved in indoor shallow soil after culture 7d and is cultivated, 30d is moved back to outdoor rice and trained
It supports and is cultivated in bucket, every barrel of plantation 5 is to be seeded.
The preparation and inoculation of 1.2 ustilaginoidea virens inoculation bacterium solution
In superclean bench, ustilaginoidea virens fungus block is inoculated in PS culture solution with transfer needle, 27 DEG C, 150r/min
Shaken cultivation 7 days, obtain a large amount of mycelia and thin-walled conidium；The mycelia in bacterium solution is smashed with tissue pulper, forms bacterium
Mixed liquor is diluted 2 times with potato liquor by silk segment and the conidial mixed liquor of thin-walled.
The inoculation liquid that 2mL is drawn with asepsis injector, from the Rice Panicle packet for being injected laterally the 6-9 d before cut is eared, directly
It is overfilled with to inoculation liquid, be most easily inoculated in period the dusk.
1.3L- leucine processing inoculation spike of rice
After Inoculated Rice heading, according to the concentration of the embodiment 1-7 L-Leu provided, prepared with sterilizing ultrapure water
0.05%, the L-Leu solution of 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4% 7 various concentration sprays spike of rice
It applies, using the ultrapure water that sterilizes as control, every group sprays 5 plants and connect bacterium spike of rice, is administered 3 times (application interval 7d) altogether, observes each processing
Between false smut morbidity difference and record every fringe rice curve quantity.Test result as shown in figure 5, L- leucine for different
Rice varieties rice curve quantity has certain influence, and the concentration of L-Leu is bigger, and the quantity of rice curve is fewer, i.e., to rice song
Germ disease incidence inhibitory effect is more significant.
2, l-Isoleucine influences different rice varieties rice curve quantity
Embodiment 8- is measured using the identical method of method is influenced on different rice varieties rice curve quantity from L-Leu
Influence of the 17 l-Isoleucine solution provided to different rice varieties rice curve quantity, wherein different according to L- in embodiment 8-17
Leucine concentration, sets 11 groups altogether, respectively control group and l-Isoleucine concentration be 0.05%, 0.1%, 0.25%,
0.5%, 1%, 2%, 4%, 8%, 16% and 32% processing group, test result as shown in fig. 6, l-Isoleucine for not
Same rice varieties rice curve quantity has certain influence, and the concentration of l-Isoleucine is bigger, and the quantity of rice curve is fewer, i.e.,
It is more significant to ustilaginoidea virens disease incidence inhibitory effect.
3, valine influences different rice varieties rice curve quantity
Embodiment 18- is measured using the identical method of method is influenced on different rice varieties rice curve quantity from L-Leu
Influence of 26 valine solutions provided to different rice varieties rice curve quantity, wherein according to valine in embodiment 18-26
Concentration, sets ten groups altogether, respectively control group and valine concentration be 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%,
4%, 8% and 16% processing group, test result is as shown in fig. 7, valine is equal for different rice varieties rice curve quantity
There is certain influence, and the concentration of valine is bigger, the quantity of rice curve is fewer, i.e., more aobvious to ustilaginoidea virens disease incidence inhibitory effect
It writes.
3 branched-chain amino acid assistant medicament of test example is to false smut control effect testing
1, L-Leu assistant medicament is to false smut control effect testing
This test is wide field trial, and test site is river in Zhejiang Province excellent 538 for examination rice varieties in the Xiaoshan City town Yi Qiao, according to
75% oxime bacterium Tebuconazole (WDG) (15g/ mus), 43% Tebuconazole (SC) (15g/ mus), 30% of embodiment 27-118 offer
Azoles alcohol (SC) (22.5g/ mus), 15% benzene first propiconazole (SC) (30g/ mus), 32.5% benzene first Fluoxastrobin (SC)
(60g/ mus), 61% jinggangmeisu (SP) (8.196g/ mus), 12.5% olefin conversion (WP) (45g/ mus), 10% difenoconazole
(WP) (30g/ mus), 15% triazolone (WP) (100 g/ mus), 21.5% Tebuconazole (OD) (30g/ mus), 15% jinggangmeisu
(OD) (33.3g/ mus), 12% thifluzamide (OD) (46g/ mus), 25% propiconazole (EC) (60mL/ mus) and blank respectively with
0.05%, the L-Leu compounding of 0.1%, 0.2%, 0.4%, 0.8%, 1.6% and 2.4% 7 various concentration and clear water are made
For control, 112 groups of processing are set altogether, the progresss first time application in 10 days before rice cut carries out second of application in 5 days before cut,
It is carried out using power spraye conventional spraying.Every group of dough stage random searching 20 clumps investigates false smut disease incidence.
False smut grade scale: 0 grade: not occurring；1 grade: every fringe infected seed 1；3 grades: every fringe infected seed 2-4；5 grades: every fringe
Infected seed 5-8；7 grades: every fringe infected seed 9-15；9 grades: every fringe infected seed 16 or more.
Note: rice in China institute Huang Shiwen.
Relative control effect=(check plot disease refers to-is prevented and treated area's disease and is referred to)/check plot disease refers to × 100%
Preventive effect is as shown in table 1: wherein anti-effectiveness hundred-mark system indicates, from table it can be concluded that, by L-Leu and embodiment
The chemical pesticide mentioned in 27-118 is applied together, and medicament can be improved to the preventive effect of false smut, improve the control efficiency of false smut,
And concentration is bigger, control efficiency is better.
Table 1, L-Leu fiting chemical pesticide are to the preventive effect of ustilaginoidea virens
2, l-Isoleucine assistant medicament is to false smut control effect testing
Using the method and preventive effect calculation method with L-Leu assistant medicament to false smut control effect testing, according to embodiment
The medicament that is there is provided in 119-248 and concentration be 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8%, 16% and
32% l-Isoleucine compounding and clear water conduct control, sets 154 groups of processing altogether, obtains the use pair of l-Isoleucine medicament for matching
Ustilaginoidea virens preventive effect as a result, as shown in table 2, wherein anti-effectiveness hundred-mark system indicate, from table it can be concluded that, by l-Isoleucine with
The chemical pesticide mentioned in embodiment 119-248 is applied together, and medicament can be improved to the preventive effect of false smut, improve the anti-of false smut
Effect is controlled, and concentration is bigger, control efficiency is better.
Table 2, l-Isoleucine fiting chemical pesticide are to the preventive effect of ustilaginoidea virens
3, valine assistant medicament is to false smut control effect testing
Using the method and preventive effect calculation method with L-Leu assistant medicament to false smut control effect testing, according to embodiment
The medicament and concentration provided in 249-365 is 0.05%, 0.1%, 0.25%, 0.5%, 1%, 2%, 4%, 8% and 16%
Valine compounding and clear water set 140 groups of processing altogether, obtain the use of valine medicament for matching to ustilaginoidea virens preventive effect knot as control
Fruit wherein anti-effectiveness hundred-mark system indicates, it can be concluded that, will mention from table in valine and embodiment 249-365 as shown in table 3
To chemical pesticide apply together, medicament can be improved, the control efficiency of false smut is improved to the preventive effect of false smut, and concentration is got over
Greatly, control efficiency is better.
Table 3, valine fiting chemical pesticide are to the preventive effect of ustilaginoidea virens
These are only the preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. application of the branched-chain amino acid in the pesticide control of preparation rice disease.
2. application according to claim 1, which is characterized in that the rice disease is rice fungus diseases evil.
3. application according to claim 2, which is characterized in that the rice fungus diseases evil is false smut.
4. application according to claim 1, which is characterized in that the branched-chain amino acid includes L-Leu, the different bright ammonia of L-
One of acid and valine are a variety of；Preferably, the branched-chain amino acid includes L-Leu, l-Isoleucine and valine
In any two kinds of mixture.
5. the pesticide control of rice disease, which is characterized in that it includes branched-chain amino acid and carrier.
6. the pesticide control of rice disease according to claim 5, which is characterized in that the branched-chain amino acid accounts for the water
The weight percent of the pesticide control of rice disease is 0.05%-32%.
7. the pesticide control of rice disease according to claim 6, which is characterized in that the branched-chain amino acid includes L- bright
One of propylhomoserin, l-Isoleucine and valine are a variety of,
Preferably, when in the pesticide control of the rice disease including the L-Leu, the L-Leu accounts for the water
The weight percent of the pesticide control of rice disease is 0.05%-2.4%；
Preferably, when in the pesticide control of the rice disease including the l-Isoleucine, the l-Isoleucine accounts for institute
The weight percent for stating the pesticide control of rice disease is 0.05%-32%；
Preferably, when in the pesticide control of the rice disease including the valine, the valine accounts for the rice disease
The weight percent of harmful pesticide control is 0.05%-16%.
8. the pesticide control of rice disease according to claim 5, which is characterized in that the carrier include water, glucose,
Lactose, Arabic gum, gelatin, mannitol, starch, magnesium trisilicate, talcum, cornstarch, keratin, colloidal silicon dioxide,
One of potato starch, sandy soil and nano-titanium dioxide are a variety of.
9. the pesticide control of rice disease according to claim 5, which is characterized in that the pesticide control of the rice disease
It further include auxiliary material, the auxiliary material is selected from one of stabilizer, thickener, colorant and fragrance or a variety of.
10. the pesticide control of rice disease according to claim 5, which is characterized in that the prevention and treatment medicine of the rice disease
Agent further includes pesticide, and the pesticide includes oxime bacterium Tebuconazole, Tebuconazole, azoles alcohol, benzene first propiconazole, the phonetic bacterium of benzene first
One in ester, jinggangmeisu, olefin conversion, difenoconazole, triazolone, Tebuconazole, jinggangmeisu, thifluzamide and propiconazole
Kind is a variety of.
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