WO2018131915A1 - Preparation for preventing or treating ginseng root-rot disease - Google Patents

Abstract

The present invention relates to: a preparation containing nanosilica for preventing or treating a ginseng root-rot disease, the preparation being capable of ameliorating or improving a symptom by an infection with Ilyonectria mors-panacis; a fertilizer containing the preparation for preventing or treating a ginseng root-rot disease; a method for preventing or treating a ginseng root-rot disease, the method comprising a step for applying the preparation on a soil in which ginseng is cultivated; and a use of nanosilica for preventing or treating a ginseng root-rot disease. The preparation for preventing or treating a ginseng root-rot disease, provided in the present invention, exhibits an effect of preventing or treating a ginseng root-rot disease even when being applied to a soil in which ginseng is cultivated, and thus the preparation can be widely utilized in the effective cultivation of ginseng through the control of a ginseng root-rot disease.

Description

Preparation for preventing or treating ginseng root disease

The present invention relates to a preparation for preventing or treating ginseng root disease, and more particularly, the present invention includes nanosilica and includes ginseng root disease causing bacteria ( Illionectria). ginseng root disease prevention or treatment agent that can alleviate or improve symptoms caused by infection of mors -panacis ), fertilizer for ginseng root disease prevention or treatment containing the agent, and treating the agent to the soil to grow ginseng It relates to a method of preventing or treating ginseng root disease, and to the use of nanosilica for preventing or treating ginseng root disease.

Ginseng is composed of roots and brains under the ground, stems, leaves, and flowers (fruits) on the ground.Because it is a perennial plant, shoots emerge from the roots of the ground every spring, and the stems die every fall. Leaves a trace of being born every year. The growth and shape of the roots differ from year to year, and the harvest occurs at 4 to 6 years. Ginseng should be cultivated in the same field for 4-6 years for a long time, and after harvesting ginseng, it will not be grown any other crops, but after 1-2 years of cultivation planned site, it will be cultivated again and it will be damaged by various diseases. It is very large and the probability of serial failure is high.

Serial disturbances are known to be caused by various fungi known to rot the roots of ginseng. Thus, fungi that rot the roots of ginseng are called ginseng root pathogen or ginseng root rot pathogen. According to a recent study by the Institute of Agricultural Research, the ginseng root cause of ginseng for more than 4 years is 35%, root fungus (root rot), gray mold 32%, stem spot disease, 15%, etc. It is evaluated as the largest enemy in ginseng production, with 21.8% of roots and 50% of 6 years. In addition, root disease is difficult to early diagnosis due to the slow translation before the disease, and because it causes disease directly in the roots as the wintering in the soil, even if the symptoms of plant wilting, the disease is already prevalent in the roots is often late to control.

The use of soil fumigants and chemical pesticides for the control of ginseng root disease is known, but it is difficult to achieve a clear effect by the above control method. Survival is very difficult because it can survive. In addition, the fumigation method is not applicable when root rot occurs due to root rot during ginseng cultivation, and there is a risk of destruction of the soil ecosystem by removing harmful microorganisms and useful microorganisms at the source. It is not easy to solve the root disease of ginseng because it is possible to abuse chemical pesticides and seriously pollute not only the grown ginseng but also the surrounding water and soil.

Recently, the development of low-toxic, eco-friendly biopesticides have been attempted. For example, Korean Patent No. 10-1531322 discloses ginseng root using a composition comprising a strain of Bacillus genus AM-1058 (KCTC-12420BP) and spores. A technique for controlling pathogens that cause rot disease is disclosed, and Korean Patent No. 10-0566265 discloses a technique for inhibiting the development of ginseng root rot disease using a Pseudomonas putida CB11 strain and a microbial fungicide containing the same. Is disclosed. However, since such biopesticides basically contain microorganisms, it is costly and time consuming to cultivate and propagate the microorganisms, and also costly and timely to distribute or preserve the manufactured biopesticides. There was a disadvantage of being low.

In order to develop a method that can prevent or treat ginseng root disease more economically, the present inventors have made efforts to reduce or alleviate the symptoms of ginseng root disease caused by infection of ginseng root pathogen when nanosilica is used. The present invention was completed.

One object of the present invention is to provide a preparation for preventing or treating ginseng root disease comprising nanosilica.

Another object of the present invention to provide a fertilizer for preventing or treating ginseng root disease comprising the above formulation.

Still another object of the present invention is to provide a method for preventing or treating ginseng root disease using the preparation.

Still another object of the present invention is to provide a use of nanosilica for preventing or treating ginseng root disease.

The ginseng root disease prevention or treatment agent provided by the present invention exhibits the effect of preventing or treating ginseng root disease even when treated in the soil to grow ginseng, can be widely used for the cultivation of effective ginseng through the control of ginseng root disease. There will be.

Figure 1a shows the treatment of nano-silica of ginseng cultivated in artificial soil (Infected) inoculated with Ginseng Root Pathogens (Healthy) and Ginseng Root Pathogens after 4 days from the date of inoculation of Ginseng Root Pathogens It is a photograph showing the result of comparing the change by quantity.

Figure 1b shows the treatment of nanosilica of ginseng cultivated in artificial soil (Infected) inoculated with Ginseng Root Pathogens (Healthy) and Ginseng Root Pathogens after 8 days from the date of inoculation of Ginseng Root Pathogens It is a photograph showing the result of comparing the change by quantity.

Figure 1c shows the treatment of nano-silica of ginseng cultivated in the artificial soil (Infected) inoculated with Healthy and ginseng root pathogen without the inoculation of Ginseng Root Pathogens after 16 days It is a photograph showing the result of comparing the change by quantity.

Figure 2a is a photograph showing the degree of damage to the roots due to the infection of Ginseng Root Pathogen, step 1 shows a normal ginseng root, step 2 shows a state that the end of the ginseng root is deformed, step 3 is the end of the ginseng root This completely deformed state, and step 4

The ginseng root represents a state of deformation up to the middle part, the fifth step represents the entire ginseng root is deformed state, and the sixth step represents a completely withered state of the ginseng root.

Figure 2b is a graph showing the results of the quantitative analysis of the results of Figure 1, in accordance with the criteria for evaluating the degree of damage to the roots due to the infection of the ginseng root pathogen.

Figure 3a is a graph showing the result of comparing the change in the net weight of the roots according to the infection of the ginseng root pathogen.

Figure 3b is a graph showing the result of comparing the change in dry weight of the roots according to the infection of Ginseng Root Pathogen.

Figure 4 is a graph showing the results of comparing the change in the content of ginsenoside per unit weight of the roots according to the infection of the ginseng root pathogen.

Figure 5a is a graph showing the results of comparing the changes in the expression level of the PgMYB3 gene expressed in the ginseng root treated with or each of ginseng root pathogen or nanosilica over time.

Figure 5b is a graph showing the results of comparing the changes in the expression level of the PgWRKY22 gene expressed in the ginseng root treated with or each ginseng root pathogen or nanosilica over time.

Figure 6a is a graph showing the results of comparing the changes in the expression level of the PgSS1 gene expressed in the ginseng root treated with or each of ginseng root pathogen or nanosilica over time.

Figure 6b is a graph showing the results of comparing the changes in the expression level of the PgSE1 gene expressed in the ginseng root treated with or each of ginseng root pathogen or nanosilica over time.

Figure 6c is a graph showing the results of comparing the change in the expression level of the PgSE2 gene expressed in the ginseng root treated with or each of ginseng root pathogen or nanosilica over time.

Figure 7a is a graph showing the result of comparing the change in the expression level of the PgCAS gene expressed over time in the ginseng root treated with the ginseng root pathogen or nanosilica, respectively or together.

Figure 7b is a graph showing the results of comparing the change in the expression level of the PgLAS gene expressed in the ginseng root treated with or each of ginseng root pathogen or nanosilica over time.

As one embodiment for achieving the above object, the present invention Nano silica  Provided is a preparation for preventing or treating ginseng root disease.

The term " nano sized silica " of the present invention means silica having a particle size of 10 to 100 nm among silicas which are silicon dioxide compounds.

In the present invention, the nano-silica shows an effect to alleviate or alleviate the symptoms of ginseng root disease appearing in ginseng grown in soil contaminated with ginseng root pathogen, the form of the nanosilica is not particularly limited, crystal (crystal) ) Phase, powder phase, paste phase, gel phase, etc., may be in any form that may be present, commonly used in the art.

The term "root rot of panax ginseng" of the present invention is also referred to as "ginseng root rot" and the root of ginseng rotten by infection of the genus Ilyonectria , a pathogenic fungus present in the soil. Refers to a disease exhibiting symptoms. The one ryonek triazole in strains representing Ilyonectria Ilyonectria mors-panacis strain and weak pathogenic exhibit strong pathogenicity panacis , Ilyonectria robusta and Ilyonectria It is classified as crassa strain, and most ginseng root disease is known to be caused by infection of Ilyonectria mors-panacis strain.

Terms of this invention, "one ryonek triazol Morse waves sheath (Ilyonectria mors - panacis strain "means one of the main pathogens of ginseng root disease, and is known to exhibit strong pathogenicity.

The content of the nanosilica contained in the preparation for preventing or treating ginseng root disease of the present invention is not particularly limited as long as it can prevent or treat ginseng root disease, but as an example, 10 to 90 weight based on the total weight of the preparation %, And as another example, from 30 to 60% by weight based on the total weight of the formulation.

Preparation for preventing or treating ginseng root disease of the present invention may further include other adjuvants, additives, and the like, in addition to the nanosilica. The type, form, and content of the other auxiliaries or additives are not particularly limited, and are generally in the art or in the related arts unless they have properties that inhibit the effects of preventing or treating ginseng root disease of the nanosilica of the present invention. Other known auxiliaries, additives can be used.

For example, the formulation of the present invention may further include a conventionally used antifungal agent and the like, and may further include excipients, diluents and the like that can be used in plant preparations. The excipients or diluents may further include surfactants such as solid carriers, liquid carriers, liquid diluents, liquefied gas diluents, solid diluents or other auxiliaries such as emulsifiers, dispersants or foaming agents which can be used in plant preparations. have.

The formulation of the present invention may be formulated into a composition in which the active ingredient nanosilica and the excipient are mixed, and all methods commonly used in the art may be applied to the formulation of the composition.

The preparation for preventing or treating ginseng root disease of the present invention may vary according to the kind or degree of a reaction to be achieved using the same, the treatment method, the treatment period, the treatment route, etc. of the composition, and It may be formulated in various dosage forms in various amounts depending on various factors including the planting site, the causative agent of ginseng root disease, the degree of contamination of the causative organism and the like and all factors to be conventionally considered in the art. For example, it may be formulated into a formulation such as a hydrating agent, granules, powders, emulsions, sprays, smokescreens, capsules, gels, and the like.

In addition, it can be prepared and used in various forms of products using the formulation of the present invention, for example, may be prepared in the form of fertilizers that can be treated in the cultivated soil of ginseng, pesticides that can be fertilized in ginseng It may also be prepared in the form of. An effective amount or formulation of the nanosilica contained in such a product can be easily determined and applied by those skilled in the art.

In another aspect, the invention comprises a formulation Ginseng root disease  Providing prophylactic or therapeutic fertilizers.

Since the ginseng root disease prevention or treatment preparation provided in the present invention can be treated in the soil grown ginseng to prevent or treat ginseng root disease, the formulation can be prepared and used in the form of fertilizer to treat the soil of ginseng culture medium.

The term "fertilizer" of the present invention is also referred to as manure, it means a nutrient substance that fertilizes the land and promotes the growth of vegetation.

In the present invention, the fertilizer containing the formulation can be used simply by treating the soil before or after the cultivation of ginseng, can also be fertilized with the spraying of pesticides.

In particular, the nanosilica contained in the formulation of the present invention can be used safely than conventional biopesticides because it is harmless to humans or livestock as well as soil.

In another aspect, the present invention comprises the step of treating the formulation to the soil for growing ginseng Ginseng root disease  Provide prevention or treatment.

According to one embodiment of the present invention, while cultivating ginseng in the soil inoculated ginseng root pathogens treated with nano silica at a concentration of 1 or 2mM, and verified the effect, it was grown in soil not treated with nano silica Ginseng is a part of the ginseng root due to the development of ginseng root disease, the shoots do not proliferate in the ginseng, while ginseng grown in the nano-silica treated soil does not cause rotten parts of the ginseng roots, It was confirmed that the level of shoots proliferated (FIGS. 1A-1C).

Therefore, it was found that the use of nanosilica can prevent or treat the onset of ginseng root disease.

In another aspect, the present invention provides the use of nanosilica for preventing or treating ginseng root disease.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example  One: Ginseng root disease  Affectionate Nanosilica  Effect Analysis

After inoculating Iryonectria mors-panacis cultured at 25 ° C. for 15 days on artificial soil (Vermiculite: Perlite: Peat Moss = 3: 1: 1, v / v / v), the pathogen was inoculated. 1.5 years old ginseng was planted in artificial soil. At this time, 1.5 years old ginseng cultivated in artificial soil without ginseng root pathogen was used as a control.

Eight roots of the cultivated ginseng were set to one group, and three groups treated with 0, 1 or 2 mM nanosilica were set to the group. While the three groups were cultivated for one month, the roots of the ginseng cultivated at 4, 8 and 16 days after the inoculation date of the ginseng root pathogen were examined and the state of the shoots grown in the ginseng. Comparison was made (FIGS. 1A-1C).

Figure 1a shows the treatment of nano-silica of ginseng cultivated in artificial soil (Infected) inoculated with Ginseng Root Pathogens (Healthy) and Ginseng Root Pathogens after 4 days from the date of inoculation of Ginseng Root Pathogens It is a photograph showing the result of comparing the change by quantity.

As shown in Figure 1a, after 4 days from the inoculation of Ginseng Root Pathogen, ginseng cultivated in the soil not treated with nanosilica due to a strong hypersensitivity reaction at the end of the root, the symptoms appear discolored, the cultivation On the other hand, after 4 days, the ginseng grown in the nanosilica-treated soil showed weak hypersensitivity reactions at the ends of the roots and did not discolor. It was confirmed that the size of shoots grown in the grown ginseng did not show any difference from the control group.

Figure 1b shows the treatment of nanosilica of ginseng cultivated in artificial soil (Infected) inoculated with Ginseng Root Pathogens (Healthy) and Ginseng Root Pathogens after 8 days from the date of inoculation of Ginseng Root Pathogens It is a photograph showing the result of comparing the change by quantity.

As shown in Figure 1b, after 8 days from the inoculation of Ginseng Root Pathogen, ginseng cultivated in the soil not treated with nano-silica appears rotting symptoms of part of the root, the shoots in the cultivated ginseng On the other hand, after 8 days, ginseng grown in the nanosilica-treated soil did not show rotting at the end of the root, and the size of shoots grown in the grown ginseng was the control group. It was confirmed that no difference was found.

Figure 1c shows the treatment of nano-silica of ginseng cultivated in the artificial soil (Infected) inoculated with Healthy and ginseng root pathogen without the inoculation of Ginseng Root Pathogens after 16 days It is a photograph showing the result of comparing the change by quantity.

As shown in Figure 1c, after 16 days from the date of inoculation of Ginseng Root Pathogen, ginseng cultivated in the soil not treated with nano-silica is softened due to the rotten areas generated at the end of the root, the root tissue of the ginseng is softened It was confirmed that the shoots did not proliferate in the cultivated ginseng, whereas after 16 days, ginseng cultivated in the soil treated with nanosilica did not have rotten portions at the ends of the roots. The size of shoots grown in the cultivated ginseng was smaller than that of the control group, but it was confirmed that the shoots were grown.

From the results of FIGS. 1A to 1C, it can be seen that the nanosilica can alleviate the symptoms of ginseng root disease caused by the ginseng root pathogen.

Example  2: Ginseng root disease  Affectionate Nanosilica  Quantitative Analysis of Effects

Example  2-1: Quantitative Analysis of the Ginseng Root Appearance

Inoculated with ginseng root pathogens set in Example 1, and classified into 1 to 6 steps, depending on the level of root damage (Fig. 2a).

Figure 2a is a photograph showing the degree of damage to the roots due to the infection of Ginseng Root Pathogen, step 1 shows a normal ginseng root, step 2 shows a state that the end of the ginseng root is deformed, step 3 is the end of the ginseng root This completely deformed state, and step 4

The ginseng root represents a state of deformation up to the middle part, the fifth step represents the entire ginseng root is deformed state, and the sixth step represents a completely withered state of the ginseng root.

Based on the classification criteria, the results obtained in Example 1 were evaluated (FIG. 2B).

Figure 2b is a graph showing the results of the quantitative analysis of the results of Figure 1, in accordance with the criteria for evaluating the degree of damage to the roots due to the infection of the ginseng root pathogen. As shown in Figure 2b, even if the ginseng not treated with ginseng root pathogens treated with nanosilica, there was no change in the appearance of the ginseng root, but when treated with ginseng root pathogens treated with nanosilica, It was confirmed that the value is greatly reduced.

Example  2-2: Quantitative Analysis of Ginseng Root Weight

Actual weight and dry weight of each ginseng root obtained in Example 1 were compared (FIGS. 3A and 3B).

Figure 3a is a graph showing the result of comparing the change in the actual weight of the roots according to the infection of Ginseng Root Pathogen, Figure 3b is a graph showing the result of comparing the change in dry weight of the roots according to the infection of Ginseng Root Pathogen.

As shown in Figure 3a and 3b, the weight of ginseng was sharply reduced when infected with Ginseng Root Pathogen, but when the nanosilica treatment, the weight of the reduced ginseng root was recovered depending on the amount of nanosilica treatment.

Example  2-3: Quantitative Analysis of Ginsenosides in Ginseng Roots

The content of ginsenosides contained in each ginseng root obtained in Example 1 was quantitatively analyzed by the content per unit weight of the root (FIG. 4).

Figure 4 is a graph showing the results of comparing the change in the content of ginsenoside per unit weight of the roots according to the infection of the ginseng root pathogen.

As shown in Figure 4, when infected with Ginseng Root Pathogen, the content of ginsenosides per unit weight contained in the ginseng root was drastically reduced, but when treated with nanosilica, ginsenoside per unit weight contained in the ginseng root was reduced. It was confirmed that the content of the side was recovered depending on the amount of nanosilica treatment.

Example  3: on the gene expression of ginseng Nanosilica  effect

Example  3-1: Jasmine acid (JA)  Quantitative Analysis of Expression Levels of Genes Influencing Signal Transduction

In ginseng root, ginseng root pathogen and nanosilica were treated separately or together, and after 4, 8 and 16 days of cultivation, the effect of Jasminic acid (JA) expressed on the cultivated ginseng root was influenced. Changes in the expression levels of the PgMYB3 gene and PgWRKY22 gene were quantitatively analyzed (FIGS. 5A and 5B).

Figure 5a is a graph showing the results of comparing the changes in the expression level of the PgMYB3 gene expressed over time in the ginseng root treated with or each of ginseng root pathogen or nanosilica, Figure 5b is a ginseng root pathogen or nanosilica Is a graph showing the result of comparing the change in the expression level of the PgWRKY22 gene expressed in each or together treated ginseng roots over time.

As shown in FIGS. 5a and 5b, when the ginseng root pathogen or nanosilica was individually treated in the ginseng root, the expression levels of the PgMYB3 gene and the PgWRKY22 gene did not change, but the ginseng root pathogen and nanosilica were added to the ginseng root. When treated together, it was confirmed that the expression levels of PgMYB3 gene and PgWRKY22 gene increased rapidly after 8 days of treatment.

Example  3-2: Quantitative Analysis of Expression Levels of Upstream Genes of Ginsenoside Synthetic Genes

The effects of the ginseng root pathogen and nanosilica on the expression level of genes acting on the terpenoid synthesis process, which is performed before the synthesis of ginsenosides, was analyzed in the biosynthesis process of ginsenosides.

In general, in the ginseng root cultivated by the method of Example 3-1, the change in the expression level of PgSS1, PgSE1 or PgSE2 gene, which is a gene acting on the terpenoid synthesis process, was quantitatively analyzed (FIGS. 6A to 6C).

Figure 6a is a graph showing the results of comparing the change in the expression level of the PgSS1 gene expressed in the ginseng root treated with or each ginseng Root Pathogen or nanosilica, Figure 6b is a ginseng Root Pathogen or nanosilica Is a graph showing the results of comparing the change in the expression level of the PgSE1 gene expressed in each or co-treated ginseng root, over time, Figure 6c is a ginseng root treated with or respectively ginseng root pathogen or nano silica , PGSE2 is a graph showing the results of comparing the expression level change of the gene over time.

As shown in Figure 6a to 6c, among the three genes that act on the terpenoid synthesis process, PgSS1 and PgSE2 genes, but when the ginseng root pathogens and nanosilica is treated together, the expression level is rapidly increased, PgSE1 gene When ginseng root pathogen and nanosilica were treated together in the ginseng root, there was no change in expression level.

On the other hand, even when the ginseng root pathogen and nanosilica were treated together in the ginseng root, the expression level of the PgSS1 gene rapidly increased after 4 or 16 days, whereas the PgSE2 gene was expressed after 8 days. It is confirmed that the difference is shown in that the level is rapidly increased.

Example  3-3: Quantitative Analysis of Expression Levels of Sterol Synthetic Genes

The effects of the ginseng root pathogen and nanosilica on the expression levels of genes that act on the synthesis of steroid structures in the biosynthesis of ginsenosides were analyzed.

In general, in the ginseng root grown by the method of Example 3-1, the change in the expression level of PgCAS or PgLAS gene, which is a gene that acts on the synthesis of steroid structure, was quantitatively analyzed (FIGS. 7A and 7B).

Figure 7a is a graph showing the results of comparing the change in the expression level of the PgCAS gene expressed over time in the ginseng root treated with or each ginseng Root Pathogen or nanosilica, Figure 7b is a ginseng Root Pathogen or nanosilica In each or co-treated ginseng root, a graph showing the result of comparing the change in the expression level of the expressed PgLAS gene over time.

As shown in Figures 7a and 7b, both genes that act on the synthesis of steroid structure, when the ginseng root pathogen and nanosilica are treated together in the ginseng root, the expression level is rapidly increased, these genes are commonly 8 days It was confirmed that the expression level rapidly increased at the time elapsed.

From the above description, those skilled in the art will appreciate that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, the embodiments described above are to be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present invention.

Claims (8)

1. Preparation for preventing or treating ginseng root disease, including nanosilica.
2. The method of claim 1,

   The nanosilica is a silica having a particle size of 10 to 100nm, formulation.
3. The method of claim 1,

   The ginseng root disease is caused by the infection of the strain of Ilyonectria morspanacis, the agent.
4. The method of claim 1,

   The nano silica is to exhibit the effect of restoring the weight of the ginseng root reduced by ginseng root disease.
5. The method of claim 1,

   The nano-silica is to exhibit the effect of restoring the content of ginsenoside per unit weight of ginseng root reduced by ginseng root disease.
6. The method of claim 1,

   The nanosilica is to exhibit the effect of restoring the weight in the ginseng root, formulation.
7. A fertilizer for preventing or treating ginseng root disease, comprising the agent of any one of claims 1 to 6.
8. A method for preventing or treating ginseng root disease, comprising the step of treating the agent of any one of claims 1 to 6 in the soil for growing ginseng.

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