KR20210085328A - Method for reducing salt stress in Panax ginseng using plant growth-promoting myobacteria - Google Patents

Abstract

The present invention relates to: a composition for inhibiting salt stress in ginseng, containing Paenibacillus yonginensis DCY84^T strain, which is a plant growth promoting bacteria (PGPR); a kit for inhibiting salt stress in ginseng, containing the composition; a method for inhibiting salt stress during the cultivation of ginseng using the composition or kit; a composition for treating a ginseng root-rot disease, containing Paenibacillus yonginensis DCY84^T strain; and a method for treating a ginseng root-rot disease using the composition. The Paenibacillus yonginensis DCY84^T strain provided in the present invention is effective in: reducing salt stress during the cultivation of ginseng; and treating a ginseng root-rot disease. Accordingly, the Paenibacillus yonginensis DCY84^T strain can be widely used for effective cultivation of ginseng.

Description

Method for reducing salt stress in Panax ginseng using plant growth-promoting myobacteria}

The present invention relates to a method for alleviating the salt stress of ginseng using plant growth promoting bacteria. More specifically, the present invention relates to a plant growth promoting bacteria (PGPR), Pennybacillus Yonginensis DCY84 ^T. ( Paenibacillus yonginensis DCY84 ^T ) A composition for inhibiting salt stress of ginseng comprising a strain, a kit for inhibiting salt stress of ginseng comprising the composition, a method of inhibiting salt stress during cultivation of ginseng using the composition or kit, It relates to a composition for treating ginseng root disease comprising the Penibacillus Yongin ensis DCY84 ^T strain and a method for treating ginseng root disease using the composition.

In the soil of ginseng rhizomes, various microorganisms conducive to plant growth and ginseng metabolism exist in the form of symbiosis. Strains known as plant-growth-promoting bacteria (PGPR) can affect plants directly or indirectly. For example, the strain can increase plant growth by supplying nutrients and hormones, generating sideropores to reduce heavy metal toxicity, and inducing systemic resistance (ISR).

Long-term monoculture of ginseng increased the prevalence of disease, resulting in a decrease in yield by up to 30-60%. It is well known that cultivated ginseng is highly susceptible to abiotic stress (salt, drought, heavy metal stress, etc.). These stress factors can deteriorate the quality of ginseng during the cultivation period of 4-6 years, and one of these stresses is salt stress. When cultivating crops in soil, fertilizers are used to promote the growth of crops. If the use of such fertilizers is excessive, nutrients contained in fertilizers accumulate in the soil, and the accumulated salts in the soil By changing the pH of the, as a result, exhibits the effect of inhibiting the growth of crops. The PGPR parasitizes the roots of plants in the soil and promotes plant growth while self-proliferating, and in this process, it can promote a resistance response to stress, so to develop appropriate microorganisms that respond to various crops and stress. Research is being actively carried out for

Under this background, the present inventors made intensive research efforts to develop a microbial agent capable of suppressing salt stress during cultivation of ginseng. As a result, the Penny bacillus Yongin ensis DCY84 ^T strain not only suppresses the salt stress of ginseng, but also the causative bacteria of ginseng root disease. It was confirmed that it exhibits the effect of treating infection of the, and completed the present invention.

Korea Patent No. 10-1720658, Novel plant growth promoting microorganisms and uses thereof Korean Patent No. 10-1862362, silicic acid or salts thereof and plant seeds treated with microorganisms and uses thereof

The main object of the present invention is to provide a composition for inhibiting salt stress of ginseng comprising a plant growth-promoting bacteria (PGPR) penibacillus Yongin ensis DCY84 ^{T strain.}

Another object of the present invention is to provide a kit for inhibiting salt stress of ginseng comprising the composition.

Another object of the present invention is to provide a method for inhibiting salt stress during cultivation of ginseng using the composition or kit.

Another object of the present invention is to provide a composition for treating ginseng root disease comprising the ^{Penibacillus Yongin ensis DCY84 T strain.}

Another object of the present invention is to provide a method for treating ginseng root disease using the composition.

One embodiment of the present invention for achieving the above object provides a composition for inhibiting salt stress of ginseng comprising a Penibacillus yonginensis ^{DCY84 T} ( Paenibacillus yonginensis ^{DCY84 T) strain.}

In the present invention, the Penibacillus yonginensis ^{DCY84 T} ( Paenibacillus yonginensis ^{DCY84 T} ) strain is not particularly limited thereto, but a strain deposited with accession number KCTC12768BP may be used.

Another embodiment of the present invention provides a kit for inhibiting salt stress of ginseng comprising the composition.

Another embodiment of the present invention provides a method of inhibiting salt stress in the cultivation of ginseng using the composition or kit.

Specifically, the method of inhibiting salt stress during ginseng cultivation of the present invention includes the step of culturing ginseng after treating the composition in soil.

Another embodiment of the present invention provides a composition for treating ginseng root disease comprising a Penibacillus yonginensis ^{DCY84 T} ( Paenibacillus yonginensis ^{DCY84 T) strain.}

As used herein, the term "root rot of panax ginseng" is also referred to as "ginseng root rot", and the root rot of ginseng is caused by infection of the genus Ilyonectria, a pathogenic fungus existing in the soil. disease that exhibits symptoms. The Illonectria genus strain is divided into a strong pathogenic Ilyonectria mors-panacis strain and a weakly pathogenic Ilyonectria panacis, Ilyonectria robusta and Ilyonectria crassa strain. is known

^{The content of the Penibacillus Yongin ensis DCY84 T} strain included in the composition for treating ginseng root disease of the present invention is not particularly limited as long as it can prevent or treat ginseng root disease, but as an example, 10 based on the total weight of the composition to 90% by weight, as another example, from 30 to 60% by weight based on the total weight of the composition.

The composition for treating ginseng root disease of the present invention may further include other adjuvants, additives, etc. in addition to the ^{Penibacillus Yongin ensis DCY84 T strain.} The type, form, and content of the other adjuvants or additives are not particularly limited, and as long as they are not of a nature that inhibits the effect of preventing or treating ginseng root disease of the ^{Penibacillus Yongin nsis DCY84 T strain of the present invention} Other adjuvants and additives commonly known in similar fields may be used.

For example, the composition of the present invention may further include a commonly used antifungal agent and the like, and may further include an excipient, a diluent, etc. that can be used in a plant preparation. The excipient or diluent may further include a solid carrier, liquid carrier, liquid diluent, liquefied gas diluent, solid diluent, or other adjuvants that can be used in plant formulations, for example, surfactants such as emulsifiers, dispersants or foaming agents. have.

The composition of the present invention can be formulated into a composition in which the active ingredient, Penibacillus Yonginensis DCY84 ^T strain, and the excipients are mixed, and as a method of formulating the composition, all methods commonly used in the art are applied. can do.

The composition for treating ginseng root disease of the present invention may vary depending on the type or degree of reaction to be achieved using the same, the treatment method of the composition, treatment period, treatment route, etc., It can be formulated in various dosage forms in various amounts according to various factors including the causative bacteria of ginseng root disease, the degree of contamination of the causative bacteria, and all factors to be considered conventionally in the art. For example, it may be formulated in the form of wettable powders, granules, powders, emulsions, sprays, smokers, capsules, gels, and the like.

In addition, by using the formulation of the present invention, it can be manufactured and used in various types of products. As an example, it can be prepared in the form of a fertilizer that can be treated in the cultivation soil of ginseng, or an agrochemical that can be applied to ginseng. It may be manufactured in the form of ^{The effective amount or formulation of the Penibacillus Yonginensis DCY84 T} strain contained in these products can be easily determined and applied by those of ordinary skill in the art.

Another embodiment of the present invention provides a method of treating ginseng root disease using the composition.

Specifically, the method for treating ginseng root disease of the present invention comprises the step of treating the composition in soil where ginseng is grown.

The Penibacillus Yongin nsis DCY84 ^T strain provided in the present invention can not only reduce salt stress during cultivation of ginseng, but also exhibit an effect of treating ginseng root disease, so it will be widely used for effective cultivation of ginseng.

1 is a photograph showing the growth promoting effect of ginseng according to the treatment concentration of the Penibacillus Yongin nsis DCY84 ^{T strain.}
2 is a photograph showing the effect of reducing the growth of ginseng according to the treatment concentration of the salt.
Figure 3 is a photograph showing the salt stress inhibitory effect of the P. Yongin ensis DCY84 ^{T strain, Figure 3a is a photograph showing the result of treatment with the Penny bacillus Yongin ensis DCY84 T} strain for 3 days, Figure 3b is the Penny bacillus Yongin ensis DCY84 ^It is a photograph showing the result of treatment with the T strain for 12 days, and FIG. 3c is a photograph showing the result of treatment with the Penibacillus Yonginensis DCY84 ^T strain for 8 weeks.
4 is a photograph showing the treatment effect of ginseng root disease according to the treatment concentration of silica nanoparticles.
5 is a photograph showing the therapeutic effect of ginseng root disease according to the mixed treatment of silica nanoparticles and Pennybacillus Yonginensis DCY84 ^{T strain.}

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1: Penibacillus Yongin nsis DCY84 on the growth of ginseng ^T Effect of strain

In the cultivation of ginseng, the Phenibacillus Yongin nsis DCY84 ^T strain was treated in soil at various concentrations, and then the growth level of ginseng was compared (FIG. 1).

1 is a photograph showing the growth promoting effect of ginseng according to the treatment concentration of the Penibacillus Yongin nsis DCY84 ^{T strain.}

As shown in FIG. 1, it was confirmed that the growth of ginseng was ^{promoted as the treatment concentration of the Penibacillus Yongin nsis DCY84 T strain increased.}

Example 2: Effect of Soil Salt on the Growth of Ginseng

After treating the soil with various concentrations of salt (Nacl) during the cultivation of ginseng, the growth level of ginseng was compared (FIG. 2).

2 is a photograph showing the effect of reducing the growth of ginseng according to the treatment concentration of the salt.

As shown in FIG. 2 , it was confirmed that the growth of ginseng was inhibited as the concentration of salts in the soil increased.

Example 3: Penibacillus Yonginnsis DCY84 on salt stress ^T Effect of strain

During the cultivation of ginseng, 300 mM of salt (Nacl) and P. Yonginensis DCY84 ^T strain were treated in the soil individually or in combination, respectively, and then the growth level of ginseng was compared ( FIGS. 3a to 3c ).

Figure 3a is ^{a photograph showing the result of treatment with the Penibacillus Yonginensis DCY84 T} strain for 3 days, Figure 3b is a photograph showing the result of treatment with the Penibacillus Yonginensis DCY84 ^T strain for 12 days, Figure 3c is a photograph showing the result of treatment with the Penibacillus Yonginensis DCY84 T strain It is a photograph showing the result of treatment with the Nsis DCY84 ^{T strain for 8 weeks.}

As shown in Figs. 3a to 3c, ^{it was confirmed that as the treatment period of the Pennybacillus Yonginensis DCY84 T} strain increased, the salt stress during cultivation of ginseng could be effectively reduced.

Example 4: Therapeutic effect of silica nanoparticles on ginseng root disease

After treating ginseng with various concentrations of silica nanoparticles infected with the causative agent of ginseng root disease, symptoms of ginseng root disease were compared (FIG. 4).

4 is a photograph showing the treatment effect of ginseng root disease according to the treatment concentration of silica nanoparticles.

As shown in Figure 4, it was confirmed that the silica nanoparticles exhibit the effect of treating ginseng root disease.

Example 5: Penibacillus Yongin nsis DCY84 on ginseng root disease ^T Effect of strain

After treating ginseng infected with the causative organism of ginseng root disease with silica nanoparticles and Pennybacillus Yonginensis DCY84 ^T strain individually or in combination, the symptoms of ginseng root disease were compared (FIG. 5).

5 is a photograph showing the therapeutic effect of ginseng root disease according to the mixed treatment of silica nanoparticles and Pennybacillus Yonginensis DCY84 ^{T strain.}

As shown in FIG. 5 , the Penibacillus Yongin nsis DCY84 ^T strain showed an effect of treating ginseng root disease alone, and it was confirmed that when used in combination with silica nanoparticles, the therapeutic effect of ginseng root disease could be doubled.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims described below and their equivalents.

Claims (6)

Penibacillus Yongin ensis DCY84 ^T ( Paenibacillus yonginensis DCY84 ^T ) A composition for inhibiting salt stress of ginseng, including a strain.
According to claim 1,
The Penibacillus yonginensis ^{DCY84 T} ( Paenibacillus yonginensis ^{DCY84 T} ) strain is a strain deposited with accession number KCTC12768BP, the composition.
A kit for inhibiting salt stress of ginseng comprising the composition of claim 1 or 2.
After treating the soil with the composition of claim 1 or 2, a method of suppressing salt stress during cultivation of ginseng, comprising the step of cultivating ginseng.
Penibacillus Yongin Nsis DCY84 ^T ( Paenibacillus yonginensis DCY84 ^T ) A composition for treating ginseng root disease, including a strain.
A method of treating ginseng root disease, comprising the step of treating the composition of claim 5 in the soil for growing ginseng.

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