CN104585236B - KN 2 and application of the complex preparation in tobacco mosaic virus (TMV) is prevented containing KN 2 - Google Patents
KN 2 and application of the complex preparation in tobacco mosaic virus (TMV) is prevented containing KN 2 Download PDFInfo
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- CN104585236B CN104585236B CN201510062266.3A CN201510062266A CN104585236B CN 104585236 B CN104585236 B CN 104585236B CN 201510062266 A CN201510062266 A CN 201510062266A CN 104585236 B CN104585236 B CN 104585236B
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Abstract
The present invention relates to technical field of pesticide, discloses KN 2 and application of the complex preparation in tobacco mosaic virus (TMV) is prevented containing KN 2.The invention discloses KN 2 can induce TMV to produce special disease resistance, effectively prevent tobacco mosaic virus disease.Complex preparation SKGM 7 containing KN 2 can effectively prevent the harm and loss of tobacco virus, which includes KN 2, viral light light and full element can zinc.Using 7 complex preparation of KN 2 or SKGM, tobacco virus (TMV), and the raising having by a relatively large margin of the yield and quality to tobacco leaf not only can be effectively prevented, produces considerable economic benefit.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly to KN-2 and the complex preparation containing KN-2 are in prevention tobacco mosaic disease
Application in poison.
Background technology
Pathogens of Tobacco Virosis is more, and route of transmission is also more complicated, at present on China tobacco or with tobacco ordinary floral leaf
Based on virosis (Tobacco mosaic virus, abbreviation TMV), proportion is about more than the 70% of whole virosis, cigarette
The host range of careless virosis (TMV) is very wide, and nearly 10 sections, 100 various plants can infect TMV and fall ill, wherein with Solanaceae,
Cruciferae, composite family are aggrieved most heavy, these crops and plant it is susceptible after often result in heavy losses.Tobacco mosaic viruses disease
It is contagious during typically by various farming operations, probability bigger, tobacco seedlings are particularly infected in the seedling shed for concentrating nursery
With cigarette strain virus infection after being ill, blade occurs dark green, light green mutually embedding mottled, therefore claims mosaic disease, and cigarette strain is fallen ill initial stage, blade flower
Leaf, plant are downgraded, and with the development of the state of an illness, leaf curling is not grown, and various necrotic plaques occurs, and cigarette strain is only the half of normal cigarette strain
Left and right, tobacco leaf substantially lose utility value.Therefore tobacco virus is the most important of tobacco leaf production and other susceptible crops
Disease.
The pesticide of the currently used prevention viroses of plant substantially has three major types, and first kind medicine mainly suppresses virus
Nucleic acid is synthesized and replicated, and suppresses virus multiplication, and this kind of medicament includes morpholine biguanidine hydrochloride, Ningnanmycin etc.；Second class medicine master
It is to promote Chlorophyll synthesis to want the mechanism of action, and promoting growth of cell, promotees cell division, promotees root growth, and this kind of medicament includes：In rape
Ester, heteroauxin, gibberellic acid etc.；Three classes medicine mainly induces plant to produce broad spectrum resistance, and this kind of medicament has amino
Oligosaccharide, fatty acid mixed, lentinan etc..These medicines have virosis certain effect, but due to inducing the mark of cause of disease
Target is too wide, lacks specificity and specialization, the effect for preventing virosis are also limited.Prevention effect of these medicines to tobacco virus
Fruit is generally below 50%.Said medicine is intersected and mixture uses, prevention effect is not more than 60%.According to statistics, at present
China every year because of tobacco virus (TMV) and caused by the losses of tobacco and other crops be up to more than 300 hundred million yuans, at present
It is sick (TMV) to control and retrieve tobacco mosaic viruses there is an urgent need for looking for the prevention preferable medicament of tobacco virus in production
Caused by crop economy lose.
KN-2 is a kind of foliar fertilizer for containing 3.6% activated silica sodium molybdate, and trade name Kang Kai 2, manufacturer is Billy
When moisten Kanggong department, the effect of its operation instruction is introduced is：Promote plant growth, hestening rooting, improve the freeze proof of crop, drought resisting and
Diseases and insect pests resistance.It is required that extension rate when spraying uses is 4000-5000 times.
The content of the invention
The present invention provides KN-2 to prevent the new opplication of tobacco mosaic virus disease.Provide the compounding containing KN-2 at the same time
Preparation, and its application in prevention tobacco mosaic virus disease.
In recent years we screening tobacco virus pesticide control on carried out extensive work, serious analysis, summarize and
Collect both at home and abroad to plant virus controlling medicament on the basis of, and be inspired in the mankind to " cocktail therapy " of AIDS virus
Successful experience, we existing virosis pesticide control is carried out recipe determination, each experiment selection and the key agents that use and
Active ingredient, concentration are shown in Table 1 respectively.
Table 1：Tobacco virus prevents recipe determination medicament kind
Table 2：Tobacco virus prevents prescription medicament species and manufacturer
Note：Numbering 4,7,8 is because belong to foliar fertilizer, therefore without registration card.
There are viral inhibitors, plant growth regulator, foliar fertilizer, organosilicon synergist etc. in these medicaments, also there is Billy
When moisten the KN-2 that Kanggong department produces.We impose on KN-2 2500 times of liquid medicine jets of dilution on the tobacco leaf plant of infection TMV, at interval of
Once, its relative control effect is 71.9% after last dispenser 20 days, is above comparison medicament Kang Kai -1 for dispenser after ten days
(42.07%), 14% full element can zinc (39.23%), the protection effect of 8% Ningnanmycin (43.36%).Prove that KN-2 can be used
In the prevention of tobacco mosaic virus disease, it is at 2500 times using there is good prevention effect to tobacco mosaic virus disease.KN-2
It can induce TMV and produce special disease resistance, other mechanism wouldn't be detailed.Therefore, present invention protection is in tobacco mosaic virus disease is prevented
Application.
The present invention also protects application of the complex preparation of KN-2 in tobacco mosaic virus disease is prevented.
Preferably, the main component of the complex preparation is KN-2, can also include plant virus inhibitor, plant growth
Two or more mixing in conditioning agent, foliar fertilizer or organosilicon synergist.
It is furthermore preferred that the plant virus inhibitor is viral light light, sows safe, Junkeduke or mixed fat copper sulphate, institute
Stating plant growth regulator or foliar fertilizer can zinc, amino-oligosaccharide, lentinan or super quick protein particle for Kang Kai -1, full element.
The present invention mixes after being diluted the single doses such as viral inhibitors, plant growth regulator, foliar fertilizer and KN-2 respectively
Match somebody with somebody, the complex preparation of tobacco mosaic virus disease is screened, the antiviral of tobacco mosaic virus (TMV) can effectively be prevented by, which filtering out, matches somebody with somebody
Square preparation SKGM-7.SKGM-7 expires element by 2500 times of KN-2 dilutions, 600 times of viral light light dilutions, 3000 times 14% can zinc
Dilution presses 1:1:It is made into after 1 mixed in equal amounts is uniform.KN-2 in manufactured antiviral complex preparation SKGM-7, viral light light,
14% full element can the mass ratio of zinc be 6：25：5.Wherein, KN-2 induces TMV to produce special disease resistance；Viral light photoresist stops virus
DNA is transcribed and protein synthesis, suppresses viral nucleic acid polymerization enzymatic synthesis；Full element can zinc can growth-promoting root, strengthen plant disease resistance.Three
Person's mixture can effectively prevent the harm of tobacco virus.
The tobacco virus pesticide control KN-2 and complex preparation SKGM-7 of the present invention can effectively prevent tobacco virus
Harm and loss, using KN-2 or SKGM-7 complex preparations, not only can effectively prevent tobacco virus (TMV), and right
The raising having by a relatively large margin of the yield and quality of tobacco leaf.With the present invention complex preparation prevent tobacco virus, input and
Output ratio is 1:23.0, i.e., the reagent cost of medication is 90 yuan/mu three times, can increase by 2077 yuan of tobacco leaf income.It is if complete
State occurs virosis by 5,000,000 mu of tobaccos (national tobacco planting area is 45,000,000 mu) and uses medicament of the present invention, then tobacco grower
Income can increase by 75.5 hundred million yuans.If use scope further expands, in infection tobacco virus (TMV)
It can be used the reagent combination of the present invention to carry out virus disease control on other crops, can also produce the economic benefit of bigger.
Embodiment
Below in conjunction with the embodiment in the present invention, the technical solution in the embodiment of the present invention is carried out clearly and completely
Description, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on this hair
Embodiment in bright, the every other implementation that those of ordinary skill in the art are obtained without making creative work
Example, belongs to the scope of protection of the invention.
Heretofore described dilution, unless otherwise instructed, refers both to be diluted with water, and extension rate is volume multiple.
Embodiment 1：
Field controling test is carried out in four society's cigarette of Pu Wei townshiies of Panzhihua City Miyi County Pu Wei townshiies dragon's pool.Trial zone is located in four
River river southwest Shen Qiu tobacco plantings area.1800 meters of height above sea level, experimental field physical features is flat, and the soil texture is yellow soil, and pH value 5.7 is weak
Acidity, the content of organic matter 3.0%.It is preceding to lie fallow.
May 6 transplant planting, planting density be 1100 plants/acre, tobacco grows fine.Experimental plot cultivation condition is basic
Unanimously.
Field cigarette strain virus inoculation：All cigarette strains are tried to confession and carry out virus inoculations within 3 days before dispenser.Identified using interior
The malicious source blade of tobacco mosaic virus (tmv) (TMV) add corundum to be ground into juice, be stained with malicious source juice to cigarette strain with disinfecting cotton swab
Blade carries out frictional inoculation.
Dosage is designed according to table 2, according to plot area, correct amount takes dose and is made into liquid, according to mu liquid volume
45L, cauline leaf spraying is carried out using HD-400 hand sprayers (pressure 45Pa, injection rate 710ml/min).Antiviral compounding system
The concentration of agent SKGM-7 is prepared after liquid by 1 for each single dose according to extension rate in table 1:1:After 1 mixed in equal amounts is uniform
Complex preparation SKGM-7.
Table 3：Field tobacco virus disease control complex preparation species and concentration
Table 4：Medicament active ingredient and the mechanism of action
Spraying time and number：Field-transplanting survives rear first time dispenser, at interval of dispenser after ten days once, Yi Gongsan
It is secondary.In May 15, May 25 and each dispenser on June 4 once.
Control time and number：10 days after last dispenser, respectively investigate an onset grade within 20 days.Prevention effect evaluation with
Subject to second.
Investigation method：
Each cell cigarette strain is all investigated, incidence is recorded by following stage division, disease index is calculated and prevention is imitated
Fruit.Observed during dispenser, whether test medicine influences the normal growth and growing way situation of tobacco plant.
Tobacco virus Seriousness gradation standard (standard GB/T/T23222-2008) is classified investigation in units of strain：
0 grade：Complete stool is disease-free；
1 grade：Lobus cardiacus veinclearing or slight floral leaf, diseased plant are downgraded without obvious；
3 grades：/ 3rd blade floral leaves but indeformable, or diseased plant are downgraded as more than 3/4ths of normal plant height；
5 grades：1/3rd to half blade floral leaf, or a small number of deformable blades, or master pulse blackening, or diseased plant is downgraded and is
3rd/2nd to four/3rds of normal plant height；
7 grades：Half is to 2/3rds blade floral leaves, or deformation or the necrosis of master arteries and veins, or it is normal strain that diseased plant, which is downgraded,
High half is to 2/3rds；
9 grades：Complete stool blade floral leaf, gross distortion or necrosis, or diseased plant are downgraded more than the half for normal plant height.
Drug effect computational methods
Economical character and yield and output value investigation
Each cell of measure respectively handles the plant height of all cigarette strains and effective leaf mainly before cigarette strain is pinched for economical character investigation
Piece quantity, calculates the average value of each processing.Yield and output value measure need to respectively handle each cell cigarette strain and harvest, pricks respectively respectively
Baking, weigh respectively, Pricing classification, then calculate and be converted into the per mu yield and per mu yield value of each processing, the data of gained with
The increase percentage for calculating each processing yield and the output value is compared in blank control.
2nd, result of the test
Every survey data and the effect such as table 4 of field control tobacco mosaic disease (TMV), each place of investigation in 20 days after dispenser
The disease index of reason, the results showed that, 2 processing of antiviral complex preparation SKGM-7 and plant virus induction of resistance agent KN-2 and
The disease of 3 comparison medicaments refers to respectively 15.29,18.79,38.72,40.63 and 37.86, and blank district disease refers to as 66.86.
It is respectively 77.13%, 71.90% with the prevention effect that this calculates antiviral 2 processing of complex preparation.Comparison medicament Kang Kai -1
Prevention effect is 42.07%, comparison medicament 14% expire element can zinc prevention effect be 39.23%；8% Ningnanmycin of comparison medicament
Protection effect is 43.36%.The result shows that the protection effect of two processing of antiviral complex preparation SKGM-7 and KN2 reaches
To more than 70%, the prevention effect than several comparison medicaments is higher by 30% or so, and relative control effect improves 59.61%.
Table 5：A variety of antiviral agents and complex preparation prevent tobacco virus (TMV) effect research table
3rd, economical character and economic benefit investigation
The average plant height of investigation in 30 days and average leaf number also indicate that SKGM-7 recipe ratio blank pair after last time dispenser
According to plant height improve 41.33%, the number of blade improves 31.35%.Tobacco leaf can increase yield more than 30%, output value increase per acre
More than 50%.Result of the test shows, the antiviral complex preparation SKGM-7 and plant virus induction of resistance agent KN-2 that we study
Two formulation efficacies it is fine, be fully able to control field tobacco virosis generation and sprawling.Our formula also fills at the same time
Divide the practical problem for considering cost accounting and being widely applied.Input and output ratio are 1:23.0, i.e. reagent cost is
10 yuan/mu, can increase by 230 yuan of tobacco leaf income.
Table 6：The economical character and yield, output value application form of each processing tobacco
4th, demonstrate and promote
The complex preparation SKGM-7 and KN-2 of the tobacco virus prevention of the present invention is in Panzhihua City and each master in Sichuan Province
Field trial and Demonstration And Extension that cigarette district is passed through 2 years are taken, Demonstration And Extension area all proves the cigarette of the present invention at 20,000 mu or so
The complex preparation SKGM-7 and KN-2 of careless virus disease control is notable to tobacco virus (TMV) prevention effect, achieves well
Economic benefit and social benefit.Demonstrate complex preparation SKGM-7 and the KN-2 prevention effect of the tobacco virus prevention of the present invention
It is stable.
It can be that professional and technical personnel in the field realize or use that the above embodiment, which is intended to illustrate the present invention, to above-mentioned
Embodiment is modified and be will be apparent for those skilled in the art, therefore the present invention includes but not limited to
The above embodiment, it is any to meet the claims or specification description, meet with principles disclosed herein and novelty,
The method of inventive features, technique, product, each fall within protection scope of the present invention.
Claims (9)
- Applications of the 1.KN-2 in tobacco mosaic virus disease is prevented.
- 2. application according to claim 1, it is characterised in that KN-2 is diluted to 2500 times of liquid and is imposed on crop.
- 3. a kind of complex preparation containing KN-2, it is characterised in that the active ingredient of the complex preparation is KN-2, and plant One or both of viral inhibitors, plant growth regulator, foliar fertilizer or organosilicon synergist any of the above ratio are mixed Close.
- 4. the complex preparation according to claim 3 containing KN-2, it is characterised in that the active ingredient of the complex preparation Can zinc for KN-2, viral light light and 14% full element；The virus light light is 30% morpholine biguanidine hydrochloride and 1% ribavirin wettable powder.
- 5. the complex preparation of KN-2 according to claim 4, it is characterised in that the KN-2, viral light light, 14% full element Can zinc mass ratio be 6：25：5.
- 6. such as application of the claim 3-5 any one of them complex preparation in tobacco mosaic virus disease is prevented.
- 7. application according to claim 6, it is characterised in that the main component of the complex preparation is KN-2, Yi Jizhi Two or more mixing in thing viral inhibitors, plant growth regulator, foliar fertilizer or organosilicon synergist.
- 8. application according to claim 7, it is characterised in that the plant virus inhibitor is viral light light, sows safe, bacterium Gram poison gram or mixed fat copper sulphate, the plant growth regulator or foliar fertilizer are Kang Kai -1, full element can zinc, amino-oligosaccharide, Lentinan or super quick protein particle；The virus light light is 30% morpholine biguanidine hydrochloride and 1% ribavirin wettable powder.
- 9. application according to claim 8, it is characterised in that KN-2 is diluted to 2500 times of liquid, viral light light is diluted to 600 times of liquid, 14% full element can zinc be diluted to 3000 times of liquid, three presses 1:1:1 volume ratio is imposed on crop after mixing.
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| CN107047615A (en) * | 2017-05-11 | 2017-08-18 | 四川省农业科学院植物保护研究所 | The combination agent and preventing control method of Synthetical prevention Prospect on Kiwifruit Bacterial Canker |
| CN108402044A (en) * | 2018-03-19 | 2018-08-17 | 四川省烟草公司广元市公司 | A kind of method for integrated control of tobacco virus |
| CN110627881B (en) * | 2019-10-17 | 2022-12-27 | 中国烟草总公司陕西省公司 | Preparation and application of hypersensitive protein for biological prevention and treatment of tobacco mosaic virus disease |
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| CN101438718A (en) * | 2008-12-30 | 2009-05-27 | 贵州大学 | Medicament for resisting plant virus and formulating method |
| CN102349538A (en) * | 2011-11-04 | 2012-02-15 | 广西田园生化股份有限公司 | Anti-plant virus pesticide composition |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101438718A (en) * | 2008-12-30 | 2009-05-27 | 贵州大学 | Medicament for resisting plant virus and formulating method |
| CN102349538A (en) * | 2011-11-04 | 2012-02-15 | 广西田园生化股份有限公司 | Anti-plant virus pesticide composition |
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Effective date of registration: 20151016 Address after: 610066 Sichuan city of Chengdu province Jinjiang District Temple Road No. 20 Applicant after: Inst of Plant Protection, Sichuan Academy of Agricultural Sciences Applicant after: Sichuan Province Company of China National Tobacco Coporation Applicant after: PANZHIHUA CITY COMPANY, SICHUAN TOBACCO COMPANY (PANZHIHUA CITY TOBACCO COMPANY) Address before: Chengdu City, Sichuan province 610066 Jingju Temple Road, No. 20 Applicant before: Inst of Plant Protection, Sichuan Academy of Agricultural Sciences |
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Inventor after: Liu Xu Inventor after: Yan Fangfang Inventor after: Jiang Wenping Inventor after: Zhang Yingjie Inventor after: Li Zhou Inventor after: Xiang Yong Inventor after: Gao Ming Inventor after: Yang Peng Inventor after: Wu Xianhua Inventor after: Zhang Wei Inventor after: Chang Ningtao Inventor after: Yao Ge Inventor after: Liu Hongling Inventor after: Chen Qingdong Inventor after: Yang Dongsheng Inventor after: Chen Song Inventor after: Lei Yinghua Inventor after: Xiao Jun Inventor after: Guan Yu Inventor after: Pan Xingbing Inventor after: Yang Junwei Inventor after: Cai Mingcai Inventor after: Wu Bin Inventor after: Yang Zhangming Inventor after: Zeng Zongliang Inventor after: Hu Xiang Inventor after: Tian Chengquan Inventor after: Xiong Weiliang Inventor after: Liu Xuedong Inventor after: Guo Mingquan Inventor after: Yu Wei Inventor after: Zhang Bingke Inventor after: Hu Jianxin Inventor after: Zeng Qingbin Inventor after: Zhang Ruiping Inventor before: Yao Ge Inventor before: Yan Fangfang Inventor before: Jiang Wenping Inventor before: Zhang Yingjie Inventor before: Li Zhou Inventor before: Xiang Yong Inventor before: Gao Ming Inventor before: Yang Peng Inventor before: Wu Xianhua Inventor before: Zhang Wei Inventor before: Chang Ningtao Inventor before: Liu Xu Inventor before: Liu Hongling Inventor before: Chen Qingdong Inventor before: Yang Dongsheng Inventor before: Chen Song Inventor before: Lei Yinghua Inventor before: Xiao Jun Inventor before: Guan Yu Inventor before: Pan Xingbing Inventor before: Yang Junwei Inventor before: Cai Mingcai Inventor before: Wu Bin Inventor before: Yang Zhangming Inventor before: Zeng Zongliang Inventor before: Hu Xiang Inventor before: Tian Chengquan Inventor before: Xiong Weiliang Inventor before: Liu Xuedong Inventor before: Guo Mingquan Inventor before: Yu Wei Inventor before: Zhang Bingke Inventor before: Hu Jianxin Inventor before: Zeng Qingbin Inventor before: Zhang Ruiping |
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