CN101438718A - Medicament for resisting plant virus and formulating method - Google Patents
Medicament for resisting plant virus and formulating method Download PDFInfo
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- CN101438718A CN101438718A CN 200810069089 CN200810069089A CN101438718A CN 101438718 A CN101438718 A CN 101438718A CN 200810069089 CN200810069089 CN 200810069089 CN 200810069089 A CN200810069089 A CN 200810069089A CN 101438718 A CN101438718 A CN 101438718A
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Abstract
The invention relates to a medication against plant virus and a preparation method thereof. Chinese toon extract, ningnanmycin and other auxiliary agents, for example, biodiesel, are taken as raw materials, and are prepared into pesticide missible oil preparation, pesticide wettable powder preparation, pesticide aqueous suspension preparation or aqueous dispersion preparation according to proportion. During the process of studying the Chinese toon extract, the invention finds that the Chinese toon extract can inhibit the plant virus diseases and has the mutual synergized action when being used together with the ningnanmycin, thereby the pesticide with high efficiency, low toxicity and small poison to the human and the environment is developed on the basis, and the pesticide can better prevent and control tobacco mosaic virus, cucumber mosaic virus diseases the corn stripe virus, rice dwarf virus diseases, and the like, and meanwhile the pesticide can overcome the problem of resistance of the plant virus to the single drug.
Description
Technical field: the present invention relates to a kind of agricultural chemicals, a kind of agricultural chemicals of Antiphytoviral, specifically a kind of is a kind of pesticidal preparations with Antiphytoviral of primary raw material preparation with Chinese toon extract and Ningnanmycin.
Background technology: the viroses of plant can be described as " cancer " of plant, and extremely difficult control brings about great losses to agricultural production throughout the year, and the loss of global annual crops reaches multi-million dollar.In recent years, virus disease endangers seriously day by day, and economic crops have especially become tobacco to produce a bottleneck problem as the harm of tobacco virus.According to estimates, Mei Nian loss just reaches several hundred million units.In addition, by the virus disease stripe disease that small brown rice planthopper is propagated, occurrence degree increased the weight of year by year in recent years, had risen to one of main disease of paddy rice.Both at home and abroad the kind of control crop disease viral disease is few, available use have only Ningnanmycin, virus of A, ZhiBingLing(SIC) etc., and control efficiency is relatively poor, common about 40～60%.
Along with the mankind to the improving constantly of self and environmental awareness, human beginning actively seek have efficiently anticancer, anti-inflammatory, the newtype drug of antiviral and desinsection.Discover, the chemical composition that contains in a lot of natural plants, have efficient, low toxicity, mankind itself and environment are poisoned little characteristics, so the chemical constitution of active ingredient in the analysis natural products is studied its structure-activity relationship, seek the natural template of synthetic, it is synthetic to carry out green, and then obtains newtype drug efficient, low toxicity.Therefore from natural products, seek lead compound, become one of focus of research and development novel agrochemical and medicine with greater activity.
Chinese toon is a kind of perennial woody plant that belongs to Meliaceae, and formal name used at school Toona sinensis (A.Juss.) Roem has another name called pig Chinese toon, spring sun-light tree, Chun Yashu, white Chinese toon, Xiang Shu.Chinese toon is Chinese endemic tree, originate in the middle part of China, mainly contain seven kinds of red Chinese toon, brown Chinese toon, red autumnal leaves Chinese toon, the green Chinese toon of red bud, dirty oil Chinese toon, chilli oil Chinese toon, blue or green oily Chinese toon and tongue Chinese toons, Chinese toon except Xinjiang, the Inner Mongol etc. outside the province, be distributed widely in all parts of the country; Next is the fruitlet Chinese toon, 9 provinces and regions distribution is arranged, and puts down in writing these provinces and regions and also produces the fruitlet Chinese toon according to record " Henan flora ", and the chances are that the fruitlet Chinese toon distributes northernmost for this; Toon and mutation hair toon thereof mostly are distributed in China west and south and minority area, east: and the Jinhua Chinese toon only finds to be distributed in areas by north, the southeast such as Zhejiang, Fujian, Jiangxi at present.At the cultivation history in existing more than 2000 year of China.Chinese toon timber often is used to make various top-grade furnitures and as the ornamental trees and shrubs plant.According to bibliographical information, the chemical composition that Chinese toon mainly contains is flavones, terpene, anthraquinone, saponin, tannin, alkaloid, aldehyde, ketone, steroidal, volatilization wet goods.
The plant of Li Xiaodong in 1999 report Melia, seal Melia in insecticidal activity, have the reactive specy quantitative aspects to be higher than other to belong to, the plant of Aglaia and Cedrela also has higher insecticidal activity.Li Xiaodong, the new development of Meliaceae plant citrin compounds insecticidal activity research, Entomologia Sinica, 1999,6 (3), 283-288.
High post galaxy of literary talent report extracted the Chinese toon saponin(e as solvent with industrial alcohol in 2008, and extract successively with benzinum, ethyl acetate, saturated n-butanol, with methyl alcohol-ether mixed liquor the Chinese toon saponin(e has been carried out purifying again, with color reaction the Chinese toon saponin(e is carried out inspection and known, adopted inhibition zone method to inquire into the Mlc and the effect of Chinese toon saponin(e.The result shows and contains triterpenoid saponin and steroid saponin in the Chinese toon old leaf that the Chinese toon saponin(e all has certain bacteriostasis to Escherichia coli, proteus, aerobacteria, and bacteriostatic activity maximum when concentration is 1.0mg/mL.(Lu Wenlin, Li Xiuxin, the extraction of Chinese toon saponin(e and the research of bacteriostatic activity, Agricultural University of the Inner Mongol's journal, 2008,29 (1), 227-229.)
Ou Yangjie adopted agar diffusion method that the antibacterial activity of chloroform, ethyl acetate and the extracting n-butyl alcohol part of Chinese toon tender shoots and old leaf extract is studied in 2008.The antibacterial activity of tender shoots extract is apparently higher than old leaf extract, and wherein the ethyl acetate of tender shoots extract partly shows the strongest antibacterial activity, and its minimal inhibitory concentration (MIC) is 1.3mg/mL.Experimental result shows that the antibacterial substance in the Chinese Toon Leaves reduces gradually along with the prolongation of growth time.(Ou Yangjie, Wu Yanwen, Lu Xiaorui, the comparative studies of Chinese toon tender shoots and old leaf extract antibacterial activity, research and development of natural products, 2008,20,427-430).
Prior art shows, the extract of Chinese toon and Chinese toon has the Pesticidal and sterilizing ability, to roundworm, trichomonad, dysentery, Escherichia coli, proteus, aerobacteria etc., certain inhibitory action is all arranged, but the application aspect Antiphytoviral is not reported.
Ningnanmycin is a kind of cytidine peptide type broad-spectrum antibiotic, is produced by streptomycete 16A-6 bacterial strain.It is reported, this medicine can effectively be prevented and treated the virus disease of various crop such as tobacco, tomato, capsicum, melon, paddy rice, wheat, vegetables, fruit tree, beans, and can prevent and treat bacterial diseases such as fungal diseases such as powdery mildew, root rot, stem rot, damping off, eqpidemic disease, climing rot, anthracnose, paddy rice culm rot and bacterial blight of rice, soft rot of cabbage, solanaceous crops bacterial wilt, can also prevent and treat diseases such as banana bunchy top disease, floral leaf heart rot, pepper mosaic disease, pawpaw ring spot, genseng damping off and stem rot, general preventive effect is 40～60%.
Summary of the invention: the present inventor finds that under study for action the Chinese toon extract is inhibited to the viroses of plant such as tobacco mosaic virus disease, maize stripe virus, rice dwarf virus disease viral disease, cucumber mosaic viral disease, potato virus X, marmor upsilon, and the present inventor finds that also the Chinese toon extract cooperates with Ningnanmycin Ningnanmycin is had synergistic effect simultaneously; Otherwise Ningnanmycin cooperates with the Chinese toon extract Chinese toon extract also there is synergistic effect, can overcomes the pesticide resistance of plant virus simultaneously.
The Chinese toon extract that the objective of the invention is to utilize the inventor to find has the character of Antiphytoviral; Utilize Chinese toon extract and Ningnanmycin Antiphytoviral that the character of synergistic effect is arranged mutually, prepare a kind of high-efficiency low-toxicity, mankind itself and environment are poisoned the medicament of little Antiphytoviral, be used to prevent and treat viral diseases of plants and overcome the pesticide resistance of virus the medicament of single control viral diseases of plants.
The present invention is to be raw material with Chinese toon extract, Ningnanmycin and other auxiliary agent, the pesticidal preparations of mixed preparing in proportion, and its weight percent proportioning is:
Chinese toon extract 1～50%, Ningnanmycin 0～50%, other auxiliary agent transfers to 100%; The preparation of indication of the present invention has missible oil, wettable powder, aqueous suspension liquor or water dispersant.
The Chinese toon extract of indication of the present invention is the skin with Chinese toon, the skin of fruit or root is a raw material, it with concentration 70～95% alcohol immersion, with soak through concentrating under reduced pressure, reclaim the ethanol extract that obtains behind the ethanol, claim the alcohol layer extract, again ethanol extract being added low amounts of water disperses in Ultrasound Instrument, earlier with chloroform extraction, inferior to ethyl acetate extraction, use extracting n-butyl alcohol at last, the various extract concentrating under reduced pressure of gained obtain medicinal extract, the chloroform layer extract, the ethyl acetate layer extract, the medicinal extract that the butanol extraction liquid concentrating under reduced pressure obtains adds ethanol extract, is referred to as the Chinese toon extract.
The Ningnanmycin of indication of the present invention is commercially available commercial medicament (production of Sichuan gold bead ecological agriculture Science and Technology Ltd.).
Other auxiliary agent of indication of the present invention, according to the difference of formulation and difference, cream preparation formulation ratio that the present invention prepared and compound method percentage by weight are calculated as: Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) surfactant 5～20%, (2) solvent 10～80%, (3) cosolvent 0.5～20%, (4) PH conditioning agent 0.5～1, (5) stabilizing agent 0.5～5%;
With the weighing in proportion of each component, total amount is 100%, puts into modulation kettle and mixes well and promptly get product.
In other auxiliary agent, (1) the surfactant comprise preparation by weight 5～20%, surfactant is the sodium alkyl sulfate of nonionic alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, alkylaryl polyoxyethylene polyoxypropylene random copolymerization ethers and anionic or in methyl sodiosul foaliphatate or the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (2) solvent accounts for preparation by weight 10～80%, and solvent is various biodiesel, one or more in the available Jatropha curcas in the source of biodiesel, Chinese tallow tree, rape seed, Chinese pistache, the moleplant seed, use when multiple, total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (3) cosolvent accounts for preparation by weight 0.5～20%, and cosolvent is one or more in methyl alcohol, ethanol, propyl alcohol, the isoamyl alcohol, uses when multiple, and total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (4) pH value conditioning agent accounts for preparation by weight 0.5～1%, and pH value conditioning agent is one or more in saleratus, acetic acid, the phosphoric acid, uses when multiple, can not be greater than the maximum of deal though always use.
In other auxiliary agent, (5) stabilizer comprises weight of formulation meter 0.5～5%, stabilizing agent are alkyl (aryl) phosphate, phosphite ester, and one or more in the polyalcohol, total usage amount is not more than the maximum of deal during use.
In the auxiliary agent, surfactant, solvent, cosolvent are main components.
Wetting powder formulation ratio that the present invention prepared and compound method percentage by weight are calculated as:
Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) wetting agent 3～35%, (2) dispersant 4～30%, (3) filler 20～70%.
With each component drying, cooling, mixing, the levigate product that promptly gets.
In other auxiliary agent, (1) wetting agent accounts for preparation by weight 3～35%, and wetting agent is natural products wetting agent and anionic wetting agent, one or more in available spaonin powder, neopelex, the washing powder, use when multiple, total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (2) dispersant accounts for preparation by weight 4～30%, dispersant is industrial by-products and anionic dispersing agent, in available lignosulfite, lignin and derivative sulfonate, naphthalene, alpha-alkyl sulfonate and the alkyl naphthalene formaldehyde condensate sulfonates one or more, use when multiple, total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (3) filler accounts for preparation by weight 20～70%, and filler is mineral carrier or synthetic vectors, and one or more in available high mountain range scholar, diatomite, the white carbon use when multiple, and total usage amount can not be greater than the maximum of deal.
Water slurry agent prescription proportioning that the present invention prepared and compound method percentage by weight are calculated as:
Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) wetting agent 5～10%, (2) dispersant 5～10%, (3) tackifier 0.2～5%, (4) stabilizing agent 0.5～5%, (5) defoamer 0.2～5%, (6) antifreezing agent 5～10%, (7) running water supplies 100%.
Each component is put into modulation kettle mixes well and promptly gets product.
In other auxiliary agent, (1) wetting agent accounts for preparation by weight 5～10%, wetting agent is one or more in aliphatic alcohol sulfate, alkylaryl sulfonates, alkylphenol polyoxyethylene, the fatty alcohol-polyoxyethylene ether, uses when multiple, and total usage amount can not be greater than the maximum of component;
In other auxiliary agent, (2) dispersant accounts for preparation by weight 5～10%, dispersant is lignin sulfonic acid salt such as sulfomethylated lignin acids such as sodium lignin sulfonate, calcium lignosulfonate, alkylnaphthalene sulfonate is as drawing back powder, during in the salt of naphthalene sulfonic acid-formaldehyde condensation product one or more, total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (3) tackifier account for preparation by weight 0.2～5%, increase viscosity and be total one or more of polyvinyl alcohol (PVA), methylcellulose, clay mineral such as Peng Run soil, macromolecular compound such as xanthans, use when multiple, total usage amount can not be greater than the maximum of deal.
In other auxiliary agent, (4) stabilizer comprises preparation by weight 0.5%～5%, stabilizing agent is one or more in xanthan gum, the Magnesiumaluminumsilicate, uses when multiple, total usage amount can not be greater than the maximum of deal.
In other auxiliary agent, (5) defoamer accounts for preparation by weight 0.2～5%, and defoamer is organosilicon ketone, C
8-C
10Fatty alcohol, C
8-C
20In saturated fatty acid and ester class thereof, the polymerization glycerine one or more use when multiple, and total usage amount can not be greater than the maximum of the maximum of deal;
In other auxiliary agent, (6) antifreezing agent accounts for preparation by weight 5～10%, and antifreezing agent is one or more in ethylene glycol, glycerine, propane diols, polyethylene glycol, the sorbierite, uses when multiple, and total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (7) running water supplies 100%.
In the auxiliary agent, wetting agent, tackifier, stabilizing agent, defoamer can replace with the organic solvent butanone, but cost is higher.
The water dispersible formulation formulation ratio of the present invention's preparation and compound method percentage by weight are calculated as:
Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) wetting agent 5～20%, (2) dispersant 5～20%, (3) binding agent 1～10%, (4) disintegrant 1～10%, (5) carrier 20～70%.
The mixture of preparation is in proportion carried out granulation after the ultra-fine grinding, drying, screening promptly get product.
In other auxiliary agent, (1) wetting agent accounts for preparation by weight 5～20%, in neopelex, dibutyl naphthalene sulfonate, dialkyl succinylsuccinate sulfonated ester, bay alcohol radical sodium sulphate, the aliphatic alcohol polyethenoxy base ether one or more are arranged, use when multiple, total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (2) dispersant accounts for preparation by weight 5～20%, and in wooden rope sodium sulfonate, calcium lignosulfonate, NNO, the K powder one or more are arranged, and uses when multiple, and total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (3) binding agent accounts for preparation by weight 1～10%, and binding agent is one or more in dextrin, gum Arabic, sodium silicate, sucrose, soluble starch, the polyvinyl alcohol, uses when multiple, and total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (4) disintegrant accounts for preparation by weight 1～10%, and disintegrant is one or more in urea, ammonium sulfate, starch, the anhydrous sodium sulfate, uses when multiple, and total usage amount can not be greater than the maximum of deal;
In other auxiliary agent, (5) carrier accounts for preparation by weight 20%～70%, carrier is one or more in attapulgite, diatomite, quicklime, magnesium lime, phosphorus lime, calcite, dolomite, gypsum, mica, talcum, liquid alabaster, clay, kaolinite, pearl kaolinite, montmorillonite, atlapulgite, the float stone, use when multiple, total usage amount can not be greater than the maximum of deal;
In the auxiliary agent, wetting agent, binding agent and carrier are main components.
From above-mentioned prescription as can be known, the present invention is that the various medicaments with the mould extract preparation of Chinese toon all have the effect of Antiphytoviral preferably alone, and are proved with testing in the back under zero the situation in the Ningnanmycin proportioning.Evidence the present invention preferably adopts following formulation ratio:
1, the cream preparation optimum formula is calculated as than percentage by weight:
The Chinese toon extract is 3%,
Ningnanmycin 3%,
Surfactant: methyl sodiosul foaliphatate is 15%,
Organic solvent: curcas biological diesel oil is 64%,
Cosolvent: methyl alcohol is 15%.
2, wetting powder optimum formula proportioning percentage calculating by weight:
The Chinese toon extract is 3%,
Ningnanmycin is 3%,
Wetting agent: neopelex is 15%,
Dispersant: the alpha-alkyl sodium sulfonate is 12%,
Filler: diatomite is 67%.
3, aqueous suspension agent optimum formula proportioning percentage calculating by weight:
The Chinese toon extract is 3%,
Ningnanmycin is 3%,
Dispersant: the alpha-alkyl sodium sulfonate is 8%,
Solvent: butanone is 20%,
Antifreezing agent: ethylene glycol is 3%,
Running water is 63%.
4, water-dispersible granules optimum formula proportioning percentage calculating by weight:
The Chinese toon extract is 3%,
Ningnanmycin is 3%,
Wetting agent: sodium lignin sulfonate is 18%,
Binding agent: polyvinyl acetate is 9%,
Carrier: diatomite is 67%.
In order to understand essence of an invention better, describe the technology contents of invention below in detail with example, but content of the present invention is not limited thereto.
Embodiment one
The preparation of Chinese toon extract:
8kg Chinese toon (skin, fruit, root) adds the ethanol of 60L80%, soaks three times (soaked 7 days for the first time, soaked 3 days for the second time, soaked for the third time 3 days), and soak through the Rotary Evaporators concentrating under reduced pressure, is obtained ethanol extract 1.5kg behind the recovery ethanol; Ethanol extract is called Chinese toon alcohol layer extract, ethanol extract adds low amounts of water and disperses in Ultrasound Instrument, at first with chloroform extraction, Chinese toon chloroform layer extract, secondly use ethyl acetate extraction again, get Chinese toon ethyl acetate layer extract, use extracting n-butyl alcohol at last, get the n-butanol layer extract, with each layer extract respectively concentrating under reduced pressure get medicinal extract, all medicinal extract comprise ethanol extract general designation Chinese toon extract, 8Kg Chinese toon skin extracts through this law, obtains the chloroform layer concentrated extract of about 0.05kg, and chloroform layer medicinal extract claims Chinese toon chloroform layer extract.Obtain the ethyl acetate layer concentrated extract of about 0.4kg, ethyl acetate layer medicinal extract claims Chinese toon ethyl acetate layer extract.Obtain the n-butanol layer concentrated extract of about 0.4kg, n-butanol layer medicinal extract claims Chinese toon n-butanol layer extract.
Because the polarity of chloroform, ethyl acetate and n-butanol increases successively, therefore can obtain the effective ingredient of opposed polarity by chloroform, ethyl acetate and n-butanol layering extraction, otherwise if extract successively with the order of n-butanol, ethyl acetate and chloroform, then almost whole effective ingredients all enter n-butanol layer, and ethyl acetate and chloroform then do not have effective ingredient and can extract.
The preparation of two: 6% Chinese toon+Ningnanmycin missible oil of embodiment, various (group) distribution ratio is:
The Chinese toon extract is 3% (weight)
Ningnanmycin 3% (weight)
Surfactant: methyl sodiosul foaliphatate is 15% (weight)
Organic solvent: curcas biological diesel oil is 64% (weight)
Cosolvent: methyl alcohol is 15% (weight)
Each component is put into modulation kettle mixes well and promptly gets product.
The preparation of three: 6% Chinese toon+Ningnanmycin wetting powders of embodiment, various (group) distribution ratio is:
The Chinese toon extract is 3% (weight)
Ningnanmycin 3% (weight)
Wetting agent: neopelex is 15% (weight)
Dispersant: the a-sodium alkyl sulfonate is 12% (weight)
Carrier: diatomite is 67% (weight)
With each component drying, cooling, mixing, the levigate product that promptly gets.
The preparation of four: 6% Chinese toon+Ningnanmycin aqueous suspension agents of embodiment, various distribution ratios are:
The Chinese toon extract is 3% (weight)
Ningnanmycin is 3% (weight)
Dispersant: the a-sodium alkyl sulfonate is 8% (weight)
Solvent: butanone is 20% (weight)
Antifreezing agent: ethylene glycol is 3% (weight)
Running water is 63% (weight).
Each component is put into modulation kettle mixes well and promptly gets product.
The preparation of five: 6% Chinese toon+Ningnanmycin water-dispersible granules of embodiment, various (group) distribution ratio is:
The Chinese toon extract is 3% (weight)
Ningnanmycin is 3% (weight)
Wetting agent: sodium lignin sulfonate is 18% (weight)
Binding agent: polyvinyl acetate is 9% (weight)
Carrier: diatomite is 67% (weight)
Mixture is carried out granulation after the ultra-fine grinding, drying, screening promptly get product.
Embodiment six
The sick activity test of Chinese toon crude extract resisting tobacco mosaic virus
The withered spot method of 1 half leaves: select the Nicotiana glutinosa of growing way unanimity, with the dilution of TMV virus crude extract, use the writing brush frictional inoculation, use flushing with clean water after the inoculation with phosphate buffer (0.1mol/LPBS).After treating that blade is done, spread liquid medicine (500 * 10 at right half leaf of blade tip
-6), spread corresponding solvent solution at a blade tip left side half leaf and compare.In illumination box, cultivate subsequently, 25 ℃ of control temperature, illumination 10000Lux observes behind the 3-4d, writes down the number of withered spot.
2 handle plant: every drug treating is established 1 strain, and 5～6 leaves of every strain are generally only done the therapeutic action of medicine to plant, therapeutic activity surpass 45% do protective effect and the passivation of crude extract to plant.
3 control drug: 2% Ningnanmycin, purchase in Sichuan gold bead ecological agriculture Science and Technology Ltd..
4 virus inoculations: tobacco mosaic virus (TMV), purchase in Wuhan Virology Institute,Chinan academy of Sciences.
5 results investigation and summing up: after cultivating 3～4d, write down respectively every leaf about the withered spot number of half leaf, be calculated as follows out the inhibiting rate of virus
Wherein: Y is the inhibiting rate of crude extract to plant virus
C is control group (Zuo Banye) withered spot number
A is compound treatment group (right half a leaf) withered spot number
The live body therapeutic action of table 1 Chinese toon crude extract
As can be seen from the table, each extract layer of Chinese toon has therapeutic activity preferably to tobacco mosaic virus disease, the therapeutic activity that is higher than the original crude extract alcohol layer of Chinese toon extract, wherein the therapeutic activity of n-butanol layer extract is best in each extract layer, near commercialization medicament Ningnanmycin, be worth further research.
The live body therapeutic action that table 2 Chinese toon crude extract mixture infects TMV
| Numbering | Compound | Treatment inhibiting rate % |
| 1 | A+B | 40.12 |
| 2 | A+C | 44.32 |
| 3 | A+D | 40.00 |
| 4 | B+C | 36.50 |
| 5 | B+D | 28.30 |
| 6 | C+D | 36.55 |
| Contrast | Ningnanmycin | 54.25 |
Annotate: A, B, C, D represent Chinese toon skin n-butanol, Chinese toon skin chloroform layer, Chinese toon skin ethyl acetate, Chinese toon fruit chloroform layer respectively.As can be seen from Table 2, each extract layer of Chinese toon back that is mixed is not improved the therapeutic activity of tobacco mosaic virus disease, not as using the activity of Chinese toon skin n-butanol extract layer separately.
Live body treatment, passivation that table 3 Chinese toon crude extract infects TMV
| Numbering | The Chinese toon extract | Protection inhibiting rate % | Treatment inhibiting rate % | Passivation inhibiting rate % |
| 1 | Chinese toon skin n-butanol layer | 57.28 | 60.99 | 81.00 |
| 2 | Chinese toon skin ethyl acetate layer | 42.23 | 42.00 | 57.23 |
| 3 | Chinese toon skin chloroform layer | 37.83 | 28.00 | 48.11 |
| Contrast | Ningnanmycin | 54.25 | 51.34 | 85.66 |
Result of the test shows that Chinese toon skin n-butanol layer extract all is higher than or is equivalent to commercialization medicament Ningnanmycin to live body treatment, passivation, the protective effect of tobacco mosaic virus disease, has further exploitation and is worth.
Table 4 Chinese toon crude extract and live body treatment, passivation that TMV is infected
Annotate: numbering 4 is respectively 500 * 10 for Chinese toon skin n-butanol layer and Ningnanmycin concentration
-6Being mixed by 1:1 obtains, and result of the test shows that Ningnanmycin and Chinese toon n-butanol extract layer are used the Chinese toon extract is had stronger synergistic effect.
The present invention finds that the Chinese toon extract has inhibitory action to the viroses of plant during with research Chinese toon extract, and the Chinese toon extract has mutual synergistic effect to develop for the basis when cooperating with Ningnanmycin a kind of efficient, low toxicity, mankind itself and environment are poisoned little agricultural chemicals, to tobacco mosaic virus, the sick plain poison of cucumber mosaic, the potato virus X disease, marmor upsilon has preventive and therapeutic effect, can overcome the pesticide resistance of plant virus to single medicine simultaneously.
Claims (10)
1. the medicine of an Antiphytoviral, it is characterized in that with Chinese toon extract, Ningnanmycin and other auxiliary agent, be formulated as the cream preparation of agricultural chemicals, wettable powder preparation, aqueous suspension preparation or aqueous dispersion preparation in proportion, its weight percent proportioning is: Chinese toon extract 1～50%, Ningnanmycin 0～50%, other auxiliary agent transfers to 100%;
2. the medicine of a kind of Antiphytoviral according to claim 1, it is characterized in that raw material Chinese toon extract is meant that the skin of skin, fruit or root with Chinese toon is a raw material, it with concentration 70～95% alcohol immersion, with soak through concentrating under reduced pressure, reclaim the ethanol extract that obtains behind the ethanol, claim the alcohol layer extract, again ethanol extract being added low amounts of water disperses in Ultrasound Instrument, successively at first with chloroform extraction, secondly with ethyl acetate extraction, with extracting n-butyl alcohol, the medicinal extract that the various extract concentrating under reduced pressure of gained obtain adds ethanol extract at last, general designation Chinese toon extract; The raw material Ningnanmycin is meant commercially available commercialization medicament, and other auxiliary agent of raw material is different and different according to formulation.
3. the medicine of a kind of Antiphytoviral according to claim 1 and compound method is characterized in that cream preparation formulation ratio and compound method are counted by weight percentage and are Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) surfactant 5～20%, (2) solvent 10～80%, (3) cosolvent 0.5～20%, (4) PH conditioning agent 0.5～1, (5) stabilizing agent 0.5～5%; With each component weighing total amount in proportion is 100% to put into modulation kettle and mix well and promptly get product;
Other auxiliary agent refers to respectively:
(1) surfactant: surfactant is the sodium alkyl sulfate of nonionic alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, alkylaryl polyoxyethylene polyoxypropylene random copolymerization ethers and anionic or in methyl sodiosul foaliphatate or the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not be greater than the maximum of deal;
(2) solvent: solvent is various biodiesel, and one or more in the available Jatropha curcas in the source of biodiesel, Chinese tallow tree, rape seed, Chinese pistache, the moleplant seed use when multiple, and total usage amount can not be greater than the maximum of deal;
(3) cosolvent: cosolvent is one or more in methyl alcohol, ethanol, propyl alcohol, the isoamyl alcohol, uses when multiple, and total usage amount can not be greater than the maximum of deal;
(4) pH value conditioning agent: pH value conditioning agent is one or more in saleratus, acetic acid, the phosphoric acid, uses when multiple, can not be greater than the maximum of deal though always use;
(5) stabilizing agent: stabilizing agent is alkyl (aryl) phosphate, phosphite ester, and one or more in the polyalcohol, total usage amount is not more than the maximum of deal during use.
4. the medicine of a kind of Antiphytoviral according to claim 1 and compound method, it is characterized in that the wettable powder formulation ratio by weight percentage be calculated as:
Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) wetting agent 3～35%, (2) dispersant 4～30%, (3) filler 20～70%;
With each component drying, cooling, mixing, the levigate product that promptly gets;
Other auxiliary agent refers to respectively:
(1) wetting agent: wetting agent is natural products wetting agent and anionic wetting agent, and one or more in available spaonin powder, neopelex, the washing powder use when multiple, and total usage amount can not be greater than the maximum of deal;
(2) dispersant: dispersant is industrial by-products and anionic dispersing agent, in available lignosulfite, lignin and derivative sulfonate, naphthalene, alpha-alkyl sulfonate and the alkyl naphthalene formaldehyde condensate sulfonates one or more, use when multiple, total usage amount can not be greater than the maximum of deal;
(3) filler: filler is mineral carrier or synthetic vectors, and one or more in available high mountain range scholar, diatomite, the white carbon use when multiple, and total usage amount can not be greater than the maximum of deal.
5. the medicine of a kind of Antiphytoviral according to claim 1 and compound method, it is characterized in that the formulation ratio of aqueous suspension preparation and compound method by weight percentage be calculated as:
Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) wetting agent 5～10%, (2) dispersant 5～10%, (3) tackifier 0.2～5%, (4) stabilizing agent 0.5～5%, (5) defoamer 0.2～5%, (6) antifreezing agent 5～10%, (7) running water supplies 100%;
Each component is put into modulation kettle mixes well and promptly gets product;
Other auxiliary agent refers to respectively:
(1) wetting agent: wetting agent is one or more in aliphatic alcohol sulfate, alkylaryl sulfonates, alkylphenol polyoxyethylene, the fatty alcohol-polyoxyethylene ether, uses when multiple, and total usage amount can not be greater than the maximum of component;
(2) dispersant: dispersant is lignin sulfonic acid salt such as sulfomethylated lignin acids such as sodium lignin sulfonate, calcium lignosulfonate, alkylnaphthalene sulfonate is as drawing back powder, during in the salt of naphthalene sulfonic acid-formaldehyde condensation product one or more, total usage amount can not be greater than the maximum of deal;
(3) tackify degree: increase viscosity and be total one or more of polyvinyl alcohol (PVA), methylcellulose, clay mineral such as Peng Run soil, macromolecular compound such as xanthans, use when multiple, total usage amount can not be greater than the maximum of deal;
(4) stabilizing agent: stabilizing agent is one or more in xanthan gum, the Magnesiumaluminumsilicate, uses when multiple, and total usage amount can not be greater than the maximum of deal;
(5) defoamer: defoamer is organosilicon ketone, C
8-C
10Fatty alcohol, C
8-C
20In saturated fatty acid and ester class thereof, the polymerization glycerine one or more use when multiple, and total usage amount can not be greater than the maximum of the maximum of deal;
(6) antifreezing agent: antifreezing agent is one or more in ethylene glycol, glycerine, propane diols, polyethylene glycol, the sorbierite, uses when multiple, and total usage amount can not be greater than the maximum of deal;
(7) running water supplies 100%.
6. the medicine of a kind of Antiphytoviral according to claim 1 and compound method, it is characterized in that preparing the formulation ratio of aqueous dispersion preparation and compound method by weight percentage be calculated as: Chinese toon extract 1～50%, Ningnanmycin 0～50%; Other auxiliary agent: (1) wetting agent 5～20%, (2) dispersant 5～20%, (3) binding agent 1～10%, (4) disintegrant 1～10%, (5) carrier 20～70%;
The mixture of preparation is in proportion carried out granulation after the ultra-fine grinding, and dry, screening promptly gets product;
Other auxiliary agent refers to respectively:
(1) wetting agent: in neopelex, dibutyl naphthalene sulfonate, dialkyl succinylsuccinate sulfonated ester, bay alcohol radical sodium sulphate, the aliphatic alcohol polyethenoxy base ether one or more are arranged, use when multiple, total usage amount can not be greater than the maximum of deal;
(2) dispersant: in wooden rope sodium sulfonate, calcium lignosulfonate, NNO, the K powder one or more are arranged, use when multiple, total usage amount can not be greater than the maximum of deal;
(3) binding agent: binding agent is one or more in dextrin, gum Arabic, sodium silicate, sucrose, soluble starch, the polyvinyl alcohol, uses when multiple, and total usage amount can not be greater than the maximum of deal;
(4) disintegrant: disintegrant is one or more in urea, ammonium sulfate, starch, the anhydrous sodium sulfate, uses when multiple, and total usage amount can not be greater than the maximum of deal;
(5) carrier: carrier is one or more in attapulgite, diatomite, quicklime, magnesium lime, phosphorus lime, calcite, dolomite, gypsum, mica, talcum, liquid alabaster, clay, kaolinite, pearl kaolinite, montmorillonite, atlapulgite, the float stone, use when multiple, total usage amount can not be greater than the maximum of deal.
7. according to the medicine of claim 1 or 3 described a kind of Antiphytovirals, it is characterized in that producing each composition weight per distribution ratio of 6% Chinese toon+Ningnanmycin missible oil and be: the Chinese toon extract is 3%, Ningnanmycin 3%;
Surfactant: methyl sodiosul foaliphatate is 15%, organic solvent: curcas biological diesel oil is 64%, cosolvent: methyl alcohol is 15%.
8. according to the medicine of claim 1 or 4 described a kind of Antiphytovirals, it is characterized in that producing 6% Chinese toon extract+Ningnanmycin wetting powder set of dispense ratio and be: the Chinese toon extract is 3%, and Ningnanmycin is 3%;
Wetting agent: neopelex is 15%, dispersant: the alpha-alkyl sodium sulfonate is 12%, filler: diatomite is 67%.
9. a kind of medicine of Antiphytoviral according to claim 1 or 5 is characterized in that producing each set of dispense ratio of 6% Chinese toon extract+Ningnanmycin aqueous suspension liquor and is: the Chinese toon extract is 3%, and Ningnanmycin is 3%;
Dispersant: the a-sodium alkyl sulfonate is 8%, solvent: butanone is 20%, antifreezing agent: ethylene glycol is 3%, and running water is 63%.
10. according to the medicine of claim 1 or 6 described a kind of Antiphytovirals, it is characterized in that producing each set of dispense ratio of 6% Chinese toon extract+Ningnanmycin water-dispersible granules and be: the Chinese toon extract is 3%, and Ningnanmycin is 3%;
Wetting agent: sodium lignin sulfonate is 18%, binding agent: polyvinyl acetate is 9%, carrier: diatomite is 67%.
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