CN107212011B - A kind of bactericidal composition for preventing and treating crop disease - Google Patents

A kind of bactericidal composition for preventing and treating crop disease Download PDF

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Publication number
CN107212011B
CN107212011B CN201710419781.1A CN201710419781A CN107212011B CN 107212011 B CN107212011 B CN 107212011B CN 201710419781 A CN201710419781 A CN 201710419781A CN 107212011 B CN107212011 B CN 107212011B
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disease
tetramycin
jinggangmycin
preventing
crop disease
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CN107212011A (en
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胡虓
王晓辉
孙刚忠
刘华梅
周波
霍瑞
龚永华
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WUHAN KERNEL BIO-TECH Co Ltd
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WUHAN KERNEL BIO-TECH Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of bactericidal compositions for preventing and treating crop disease, belong to technical field of pesticide.The bactericidal composition is compounded by tetramycin with jinggangmycin A, and the mass ratio of the tetramycin and jinggangmycin A is 1: 1-60.The present invention not only increase to rice sheath blight disease, false smut, capsicum damping-off, wheat scab, bacterial leaf streak of rice, cucumber bacterial angular leaf spot preventive effect and to Target crops safety, and expand fungicidal spectrum, extend the lasting period, to advantageously reduce cost, productivity effect is improved, social development is had a very important significance.

Description

A kind of bactericidal composition for preventing and treating crop disease
Technical field
The invention belongs to pesticide fields, and in particular to a kind of bactericidal composition for preventing and treating crop disease.
Background technique
Tetramycin (Tetramycin) is also known as tetramycin, is by S. ahygroscopicus Wuzhou subspecies (Streptomyces Ahygroscopicus subsp.Wuzhouensis n.subsp) fermentation generate metabolite, containing tetramycin A1, A2 with And four components of albonoursin and simple fragrant bacterium element, low toxicity, absorbability is strong, bactericidal activity is high, wide sterilization spectrum, to sac fungus, load Daughter bacteria and Fungi Imperfecti and Gram-negative, positive bacteria have extremely strong killing effect.To rice in agricultural disease prevention and treatment Banded sclerotial blight, wheat scab, capsicum damping-off, fruit tree putrefaction disease, canker, alternaria leaf spot of apple, cotton yellow (withered) wither disease, Root rot, ginseng and Alternaria panax, tealeaves gall of tea, fruit white rot of grape etc. have preferable control efficiency, and have rush Into callus healing, weak seedling well developed root system, aging root regeneration, the features such as disease resistance.
Jinggangmeisu is by Jinggangmycin (Streptomyces hygroscopicus Var.jingganggensis) a kind of aminoglycoside antibiotics generated, main component have A, B, C, D, E, F, G, H, Each component has different degrees of inhibitory activity to germ.China's jinggangmeisu yield is big, wide using area, and has and be produced into The features such as this is low, safety, low toxicity, it is prevention and treatment rice sheath blight disease, false smut, efficiently, the ideal biological pesticide of low toxicity, is being promoted Between nearly 40 years of application, significant contribution is made that the high yield of China's rice, stable yields.But conventional application jinggangmeisu component is more, Dosage is big, and preventive effect is unstable, and the lasting period is short.
Chemical pesticide is prevention and treatment plant pest the most effective means during long-term agricultural production practice, but The problem of most easily producing is pesticide residue, environmental pollution and the generation of pest and disease damage drug resistance.And in contrast, biological pesticide Have the characteristics that less toxic, environmentally friendly, pollution-free.The new varieties and compound preparation for developing biological pesticide are increasingly by the weight of people Depending on increasing developing efforts one after another.And two kinds of effective components being related in the present invention are biological pesticide, we are indoors It on the basis of screening and field trial, filters out tetramycin and is compounded with well ridge rhzomorph A, there is apparent synergistic effect, energy Usage amount is reduced, expands fungicidal spectrum, reduces the dispenser cost of peasant.Compound synergic about tetramycin and jinggangmycin A at present Bactericidal composition there is no relevant report.
Summary of the invention
The object of the present invention is to provide a kind of bactericidal compositions for preventing and treating crop disease, improve drug effect, delaying to realize Learn to farm medicine drug resistance, reduce the purpose of cost.
Bactericidal composition provided by the invention is made of tetramycin and jinggangmycin A, the tetramycin and jinggangmycin A Mass ratio is 1: 1-60.
Preferably, the mass ratio of the tetramycin and jinggangmycin A is 1: 15.
It is further preferred that the crop disease is rice sheath blight disease, false smut, capsicum damping-off, wheat scab, water Rice bacterial stripe, cucumber bacterial angular leaf spot.
A kind of pesticide for preventing and treating crop disease, active constituent is above-described bactericidal composition, furtherance supplemented by remaining Point.
A method of prevention and treatment crop disease, including by 24-48 grams/ha of active constituent formulation rate to field spray with The step of upper pesticide.
Preferably, it is sprayed by 36 grams of active constituent/hectare formulation rates.
The beneficial effects of the present invention are:
(1) present composition is through virulence determination, to the co-toxicity coefficient of Rhizoctonia solani Kuhn 120 or more, shows bright Aobvious synergistic effect, therefore the preventive effect to crop disease can be enhanced, disease incidence is reduced, the usage amount of pesticide is reduced.
(2) wide sterilization spectrum of the present invention, to Target crops safety, the lasting period is long.
(3) effective component of the invention is biological pesticide, has the characteristics that low toxicity and low residue, environmentally friendly, and Between each other without apparent cross resistance, the generation of current chemical pesticide resistance can be delayed, reduce production cost.
Specific embodiment
Below by way of specific embodiment, the present invention is described in detail, effect experiment of the invention using indoor biometrics and The mode that field trial combines, unless otherwise instructed, ratio mentioned below are all weight ratio.
Embodiment one: the co-toxicity of tetramycin and jinggangmycin A experiment
Test target: for rice sheath blight disease, (Rhizoctonia solani Kuhn, Thanatephorus cucumeris are big by Central China agricultural It learns and provides).
Test material: it tetramycin (tetramycin): is provided by Liaoning Wei Ke bioengineering limited liability company.Well ridge is mould Plain A (validamycin A) is provided by Wuhan Kenuo Biology Science & Technology Co., Ltd..
Test method: NY/T1156.18-2013 E medium therapy is used.On the basis of preliminary experiment, to contain The aqueous solution of 0.05% Tween 80 is solvent, takes the medical fluid of each single dose and mixture series of concentrations under aseptic technique, according to The sterilizing E culture medium melted in advance is quantitatively adding in sterile conical flask by test process, successively quantitative from low concentration to high concentration Medical fluid is drawn, is separately added into conical flask and sufficiently shakes up, be then directed respectively into 4 culture dishes, every ware (diameter 9cm) 15ml, The drug containing tablet of respective concentration is made, and sets without medicament but the processing that contains Tween 80 aqueous solution is as control, every processing 4 It repeats.Rhizoctonia solani Kuhn is inoculated in PDA plate center in advance, in 28 DEG C of culture 48h, is obtained using inoculation center as the center of circle Bacterium colony is demarcated in bacterium colony growing edge, to survey virulence region at the description to be located at bacterium colony growing edge, in virulence to be measured Region is beatened to take bacteria cake to guarantee that the growth potential of bacteria cake is consistent.The bacteria cake that will be prepared, be aseptically inoculated in respectively containing The E culture medium flat plate center of various concentration medicament, mycelia is face-down, covers ware lid, is placed in 28 DEG C of incubators and cultivates.
Investigation method: experimental investigation is as a result, using crossing method when blank control colony diameter grows to 75-80mm With calliper to measure colony diameter, inhibiting rate is calculated compared with control treatment.And variant proportion is calculated with SPASS software Toxicity regression curve equation y=a+bx, and effectively inhibit in concentration EC50Value, correlation coefficient r and 95% confidence limit and its mixed The co-toxicity coefficient of agent.And it is evaluated mixed effect using Sun Yunpei's Method calculating co-toxicity coefficient (CTC), mixture when CTC value is greater than 120 It is antagonism when less than 80 with synergistic effect, there is summation action when between 80-120.Related calculation formula is such as Under:
Survey toxicity index (ATI)=standard agent EC50/ reagent agent EC50*100
Theoretical mixture toxicity index (TTI)=(percentage composition of A in the toxicity index * mixture of A)+(toxicity index * of B The percentage composition of B in mixture)
Co-toxicity coefficient (CTC)=mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI) * 100.
1 tetramycin of table is with well ridge rhzomorph A to the toxicity test result of Rhizoctonia solani Kuhn
Medicament title EC50(mg/L) Actual toxicity index Theoretical toxicity index Co-toxicity coefficient
Tetramycin (A) 0.2102 100 100 --
Jinggangmycin A (B) 10.3887 2.0234 2.0234 --
A:B(1:60) 4.7246 4.4491 3.6295 122.58
A:B(1:45) 4.1759 5.0336 4.1533 121.20
A:B(1:22.5) 2.5873 8.1243 6.1926 131.19
A:B(1:15) 1.5173 13.8536 8.1469 170.05
A:B(1:11.25) 1.5186 13.8417 10.0215 138.12
A:B(1:9) 1.2882 16.3173 11.8210 138.04
A:B(1:1) 0.3246 64.7566 51.0117 126.94
As shown in Table 1, tetramycin and jinggangmycin A mixture are between 1:1-1:60, to rice sheath blight disease (Thanatephorus cucumeris) co-toxicity coefficient shows apparent synergistic effect 120 or more.According to tetramycin and Jinggangmycin A Costco Wholesale, wherein mass ratio is that synergy is the most significant in 1:15, and cost is minimum.
Then tetramycin is compounded with jinggangmeisu B, C, D, E, F, H in the ratio of 1:15 respectively, is detected according to the above method Virulence the results are shown in Table 2.
2 tetramycin of table is with jinggangmeisu B, C, D, E, F, G, H to the toxicity test result of Rhizoctonia solani Kuhn
As shown in Table 2, tetramycin compounds no synergistic effect with other jinggangmeisu components.
Embodiment two: the field control effectiveness test of present invention prevention and treatment rice sheath blight disease
Trial drug: tetramycin: jinggangmycin A (effective component ratio)=1:15, sodium benzoate 0.3% (preservative) are gone Ionized water complements to 100% (solvent), stirs and evenly mixs rear canned.
Test basic condition: test site is Jiangsu Province, Hongze County Renhe Town, Huai'an Linze village, and the time is in August, 2016 11, four repetitions of each processing, cell random alignment are tested, each plot area is 40-50m2, medicament is in rice sheath blight disease Early period of origination is watered even spraying using knapsack sprayer.Investigation method: each cell diagonal line determines two o'clock, before every straight line Into fixed 25 clumps, 7 days after application, investigates within 14 days each cell disease and refer to, calculate preventive effect, and carry out Deng Kenshi and newly answer range analysis.
Disease index=∑ (disease number of sheets * at different levels are with respect to value of series)/(investigation total number of sheets * 9) * 100
Control efficiency (%)=(disease index after disease index-treatment region medicine after blank district medicine)/blank district Disease index * 100 after medicine.
3 tetramycin jinggangmycin A of table prevents and treats rice sheath blight disease field control effectiveness test result
From table 3 it can be seen that prevention and treatment rice sheath blight disease, 7 days of the invention, 14 days control efficiency are respectively 83.79- 88.20%, 82.71-86.16%, preventive effect decline in 14 days is unobvious, and 14 days preventive effects of single dose tetramycin or jinggangmycin A Decline clearly, illustrates that the present invention can extend the lasting period.In addition, by control efficiency after 24-48 grams/ha of effective component application It is significantly better than that single dose tetramycin or jinggangmycin A, after formulation rate reaches 36 grams/ha, continues growing the increasing of dose preventive effect Add it is unobvious, therefore optimal formulation rate be 36 grams/ha.
Embodiment three: the field control effectiveness test of present invention prevention and treatment false smut
Test basic condition: test site is that Nanchang City, Jiangxi Province creates the town the Qu Liuhu village Lian Tang, tests each processing four A repetition, cell random alignment, each plot area are 40-50m2, spraying time is for the first time (the rice on the 29th of August in 2016 5 days before stem fringe cut), second is on September 15th, 2016 (full heading time), is watered even spraying using knapsack sprayer.
Investigation method: each cell looks into 20 clumps using parallel lines jump method, is investigated using 0-9 grades of grade scales, 0 Grade: without infected seed;1 grade: 1 curve of every fringe;3 grades: 2 curves of every fringe;5 grades: 3-5 curve of every fringe;7 grades: every fringe 6-9 bent Ball;9 grades: more than 10 curves of every fringe;14 days investigation prevention effects after second of medicine are investigated each cell disease and are referred to, preventive effect is calculated.
Disease index=∑ (disease spike number * at different levels are with respect to value of series)/(investigating total spike number * 9) * 100
Control efficiency (%)=(disease index after disease index-treatment region medicine after blank district medicine)/blank district Disease index * 100 after medicine.
4 tetramycin jinggangmycin A of table prevents and treats false smut field control effectiveness test result
From table 4, it can be seen that the present invention is 82.50- by 14 days control efficiency after 24-48 grams/ha of second of application 83.89%, preventive effect is substantially better than single dose tetramycin or jinggangmycin A.
Example IV: the field control effectiveness test of present invention prevention and treatment wheat scab
Test basic condition: test site is Jiangsu Province, Guanyun County, the Lianyungang Xu Zhuang Village Xing Zhen, and the time is in May, 2016 7 days, May 14, four repetitions of each processing, cell random alignment are tested, each plot area is 40-50m2, first time medicine Agent is watered even spraying using knapsack sprayer in wheat heading flowering initial stage, per acre water consumption 30-45kg, second of application After 7 days.
Investigation method: in wheat milk stage, 5 points of every cell grab sample, every 100 fringes observe and predict tune by gibberella zeaze petch of wheat and barley Specification national standard (GB/T15796-1995) is looked into be classified.Grade scale is 0 grade: disease-free;1 grade: morbidity small ear Zhan Quansui 1/4 or less;2 grades: the 1/4-1/2 of morbidity small ear Zhan Quansui;3 grades: the 1/2-3/4 of morbidity small ear Zhan Quansui;4 grades: morbidity is small 3/4 or more of fringe Zhan Quansui.It investigates each cell disease to refer to, calculates preventive effect.
Disease index=∑ (disease number of sheets * at different levels are with respect to value of series)/(investigation total number of sheets * 4) * 100
Control efficiency (%)=(disease index after disease index-treatment region medicine after blank district medicine)/blank district Disease index * 100 after medicine.
5 tetramycin jinggangmycin A of table prevents and treats wheat scab field control effectiveness test result
As can be seen from Table 5, the present invention is 71.30-77.49% to the preventive effect of wheat scab, hence it is evident that is better than single dose four Mycin and jinggangmycin A.
Embodiment five: the field control effectiveness test of present invention prevention and treatment capsicum damping-off
Test basic condition: test site is Hubei Province's Dangyang City Yuquan Jinsha village, office, and the time is April 7 in 2016 Day, four repetitions of each processing, cell random alignment are tested, each plot area is 40-50m2, there are about 120-150 for every cell Strain, medicament carry out root irrigation, every plant of irrigation liquid 200ml 7 days after capsicum is transplanted.Investigation method: each cell investigation is applied 14 days investigation prevention effects after medicine, Investigate incidence calculate preventive effect.
Disease incidence=disease plant number/each cell investigates total plant number * 100
After control efficiency (%)=(disease incidence after disease incidence-treatment region medicine after blank district medicine)/blank district medicine Disease incidence * 100.
6 tetramycin jinggangmycin A of table prevents and treats capsicum damping-off field control effectiveness test result
As can be seen from Table 6, it is 2250-4500 times of liquid, 14 days preventive effects after medicine that the present invention, which carries out concentration when root irrigation, 81.03-87.57%, hence it is evident that be better than single dose tetramycin and jinggangmycin A.
Embodiment six: the field control effectiveness test of present invention prevention and treatment bacterial leaf streak of rice
Test basic condition: test site is Your Highness village, Jiangxi Province, the township San Tang, Yugan County, tests four repetitions of each processing, Cell random alignment, each plot area are 40-50m2, first time medicament is in the rice booting initial stage on the 11st of August in 2016, and second Secondary application is on August 18th, 2016, is watered even spraying using knapsack sprayer, per acre water consumption 30-45kg.
Investigation method: 14 days after being administered within 7 days and second after first time is administered, every cell is taken using 5 diagonal lines Sample method, every investigation 25 from.By bacterial leaf streak of rice field control effectiveness test criterion national standard (GB/T17980.105- 2004) it is classified.Grade scale is 0 grade: disease-free;1 grade: blade only has the translucent water stain shape scab of dot, accounts for leaf area 1% Below;3 grades: blade has fragmentary short and fillet scab, accounts for leaf area 1%-6%;5 grades: leaf spot lesion is more, accounts for leaf area 6%- 25%;7 grades: leaf spot lesion is closeer, accounts for leaf area 26%-50%;9 grades: leaf spot lesion gathers, and accounts for 51% or more leaf area, leaf Piece becomes tenne, crimps, is withered.It investigates each cell disease to refer to, calculates preventive effect.
Disease index=∑ (disease number of sheets * at different levels are with respect to value of series)/(investigation total number of sheets * 9) * 100
Control efficiency (%)=(disease index after disease index-treatment region medicine after blank district medicine)/blank district Disease index * 100 after medicine.
7 tetramycin jinggangmycin A of table prevents and treats bacterial leaf streak of rice field control effectiveness test result
As can be seen from Table 7,7 days after the present invention is administered for the first time by 24-48 grams/ha of effective component, after second of medicine 14 days control efficiency are respectively 79.10-83.45%, 75.48-80.19%, are significantly better than that single dose tetramycin and jinggangmeisu A, and play the role of extending the lasting period, the report of jinggangmycin A prevention and treatment bacterial leaf streak of rice is had not been reported at present, illustrates this Invention expands fungicidal spectrum.
Embodiment seven: the field control effectiveness test of present invention prevention and treatment cucumber bacterial angular leaf spot
Test basic condition: test site is Hainan Province, the farm Jin An, Dengmai County, tests four repetitions of each processing, cell Random alignment, each plot area are 30m2, on November 10th, 2016, cucumber is in bloom early period first time medicament, and second Secondary application is on November 17th, 2016, is watered even spraying using knapsack sprayer, per acre water consumption 45kg.
Investigation method: 14 days after being administered within 7 days and second after first time is administered, every cell is taken using 5 diagonal lines Sample method, investigates whole blades by 3 plants every.By cucumber bacterial angular leaf spot field control effectiveness test criterion national standard (GB/ T17980.110-2004 it) is classified.Grade scale is 0 grade: disease-free;1 grade: lesion area account for the 5% of entire leaf area with Under;3 grades: lesion area accounts for the 6%-10% of entire leaf area;5 grades: lesion area accounts for the 11%-20% of entire leaf area;7 Grade: lesion area accounts for the 21%-50% of entire leaf area;9 grades: lesion area accounts for 51% or more of entire leaf area.Investigation is each Cell disease refers to, calculates preventive effect.
Disease index=∑ (disease number of sheets * at different levels are with respect to value of series)/(investigation total number of sheets * 9) * 100
Control efficiency (%)=(disease index after disease index-treatment region medicine after blank district medicine)/blank district Disease index * 100 after medicine.
8 tetramycin jinggangmycin A of table prevents and treats cucumber bacterial angular leaf spot field control effectiveness test result
As can be seen from Table 8,7 days after the present invention is administered for the first time by 24-48 grams/ha of effective component, after second of medicine 14 days control efficiency are respectively 79.52-85.18%, 76.39-82.33%, are significantly better than that single dose tetramycin and jinggangmeisu A, and play the role of extending the lasting period, and have not been reported jinggangmycin A prevention and treatment bacterial leaf streak of rice, illustrate expansion of the present invention Big fungicidal spectrum.
In conclusion the present invention is not only increased by compounding tetramycin with jinggangmycin A to rice sheath blight disease, rice Bent disease, the preventive effect of capsicum damping-off, wheat scab, bacterial leaf streak of rice, angular leaf spot of cucumber and safe to Target crops, And fungicidal spectrum is expanded, the lasting period is extended, to advantageously reduce cost, productivity effect is improved, has to social development Highly important meaning.

Claims (4)

1. a kind of bactericidal composition for preventing and treating crop disease, it is characterised in that: be made of tetramycin and jinggangmycin A, described four The mass ratio of mycin and jinggangmycin A is 1: 15, and the crop disease is rice sheath blight disease, false smut, capsicum damping-off, wheat Head blight, bacterial leaf streak of rice, cucumber bacterial angular leaf spot.
2. a kind of pesticide for preventing and treating crop disease, it is characterised in that: the active constituent of the pesticide is described in claim 1 kills Bacteria composition, remaining is auxiliary element.
3. a kind of method for preventing and treating crop disease, it is characterised in that: pesticide as claimed in claim 2 is sprayed to field, by 24-48 The sprinkling of gram active constituent/hectare formulation rate.
4. the method for prevention and treatment crop disease as claimed in claim 3, it is characterised in that: by 36 grams of active constituent/hectare applications Amount sprinkling.
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CN109805014A (en) * 2017-11-20 2019-05-28 贵州省辣如意食品有限责任公司 A kind of formulation of pesticide for promoting pepper plant to grow tall
CN115710144B (en) * 2022-12-01 2024-04-30 贵州省烟草公司贵阳市公司 Pesticide-fertilizer mixed combination for preventing and treating tobacco target spot disease and application thereof
CN116210710A (en) * 2023-03-15 2023-06-06 陕西康禾立丰生物科技药业有限公司 Tetramycin-containing bactericidal composition

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Publication number Priority date Publication date Assignee Title
CN103583532A (en) * 2013-11-26 2014-02-19 辽宁微科生物工程有限公司 Tetramycin complex and application

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103583532A (en) * 2013-11-26 2014-02-19 辽宁微科生物工程有限公司 Tetramycin complex and application

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