CN106472595B - A kind of biological agent and preparation method thereof of prevention wheat powdery mildew - Google Patents
A kind of biological agent and preparation method thereof of prevention wheat powdery mildew Download PDFInfo
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- CN106472595B CN106472595B CN201610830036.1A CN201610830036A CN106472595B CN 106472595 B CN106472595 B CN 106472595B CN 201610830036 A CN201610830036 A CN 201610830036A CN 106472595 B CN106472595 B CN 106472595B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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Abstract
The invention belongs to technical field of pesticide preparations, and in particular to a kind of biological agent and preparation method thereof of prevention wheat powdery mildew, the raw material group of the biological agent become；20-30 parts of potato extract, 2-5 parts of sodium butyrate, 8-10 parts of fatty alcohol, 10-15 parts of polyoxyethylene sorbitol acid anhydride ester, 2-3 parts of D-sorbite, 1-2 parts of ammonium sulfate, 0.8-1.2 parts of polyvinyl alcohol；The potato that the present invention is livid purple using skin and flesh, germinates, realizes the recycling of resource, turns waste into wealth, and reduces the influence to environment；Biological agent medicament residue prepared by the present invention is low, and high for wheat powdery mildew cure rate, the drug effect phase is long, is conducive to environment sustainable development, the growth after wheat can be promoted to fully recover, and can defend pathogen infecting again from wound intrusion and wound, increasing both production and income.
Description
Technical field
The invention belongs to technical field of pesticide preparations, and in particular to a kind of biological agent and its system of prevention wheat powdery mildew
Preparation Method.
Background technology
Wheat powdery mildew is the common disease of wheat, can be fallen ill within wheat entire breeding time, in recent years, in the Yellow River and Huai He River wheat
Serious harm occurs for area.Currently, wheat it is universal to the resistance of powdery mildew it is poor in the case of, chemical control is at control disease
Important means.In order to which to resist powdery mildew of wheat, the pharmaceutical preparations such as Fluoxastrobin, triazolone, but these drugs are currently mainly used
Environmental pollution has been aggravated first, secondly, easily causes the residual of pesticide, and with the enhancing of germ drug resistance, drug uses agent
Amount increases, and bacterium is also easy to produce drug resistance, and efficiency time is short.
Potato is one of daily favorite food of people.But the livid purple greening of skin and flesh it is immature or germination potato, food
With easily causing to be poisoned later, feel throat itch, chest fever pain, Nausea and vomiting etc., even if these potato working processes
After cannot guarantee that nontoxic, therefore, abandoned mostly, that is, cause the waste of resource, meanwhile, aggravated the negative of surrounding enviroment
Load.
Invention content
In view of the problems of the existing technology, the present invention provides a kind of biological agents of prevention wheat powdery mildew.
The present invention also provides a kind of preparation methods of the biological agent of prevention wheat powdery mildew.
Used technical solution is the present invention to achieve the goals above：
The present invention provides a kind of biological agents of prevention wheat powdery mildew, are made of the raw material of following parts by weight：Horse
20-30 parts of bell potato extract, 2-5 parts of sodium butyrate, 8-10 parts of fatty alcohol, 10-15 parts of polyoxyethylene sorbitol acid anhydride ester, sorbose
2-3 parts of alcohol, 1-2 parts of ammonium sulfate, 0.8-1.2 parts of polyvinyl alcohol.
Potato extract used in the present invention is extracted using following methods：Skin and flesh is livid purple, germination potato beats
It is broken, it is ground into potato pulp, ethyl alcohol-ethylene glycol is then added, zinc chloride filters after stirring 2h, obtains filtrate I；It will add in filter residue
Enter water, the complex enzyme of amylase, glucoamylase composition is added, hydrolyzes 7-8h at 36-38 DEG C, enzyme deactivation at 120 DEG C, stirring
It after 5min, dries in the air to room temperature, filtrate I and methylisothiazolinone is added, continue to stir 10min, filtering obtains filtrate.
Further, the volume ratio of the same ethyl alcohol-ethylene glycol of the potato pulp is 1:2；Body of the ethyl alcohol with ethylene glycol
Product is than being 1:1.
The addition of above-mentioned zinc chloride accounts for the 0.12-0.15% of potato pulp weight.
Further, the filter residue is 1 with the mass ratio of water:2.
Further, the addition of the complex enzyme accounts for the 0.5-0.8% of potato weight.
In complex enzyme used herein, the mass ratio of amylase and glucoamylase is 1:0.5.
Further, the addition of the methylisothiazolinone accounts for the 0.1% of potato pulp weight.
The present invention also provides a kind of preparation methods of the biological agent of prevention wheat powdery mildew, are as follows：
（1）By sodium butyrate, fatty alcohol, polyoxyethylene sorbitol acid anhydride ester, D-sorbite, polyvinyl alcohol are uniform, obtain
Solution A；
（2）After potato extract, ammonium sulfate and polyvinyl alcohol, it is warming up to 30-35 DEG C, 30min is stirred, obtains molten
Liquid B；
（3）After solution A is mixed with solution B, after stirring 30min under conditions of 1500r/min.
Contain solanine in the potato that skin and flesh is livid purple, germinates, is a kind of toxic glycoalkaloid, solanine is to Mr. Yu
The pathogenic microorganisms such as a little fungi, bacteriums have certain inhibition and toxic action, and around the callus of plants wound surface
And its accumulation in the adjacent separate living tissue re-formed, it can prevent pathogen from invading and infecting again with certain from wound
Defense reaction.
Beneficial effects of the present invention are：
（1）The potato that the present invention is livid purple using skin and flesh, germinates, realizes the recycling of resource, turns waste into wealth, and reduces
Influence to environment；
（2）Biological agent medicament residue prepared by the present invention is low, and high for wheat powdery mildew cure rate, the drug effect phase is long, profit
In environment sustainable development, the growth after wheat can be promoted to fully recover, and pathogen can be defendd from wound intrusion and wound
It infects again, increasing both production and income.
Specific implementation mode
Below by embodiment, the outstanding feature and marked improvement that the present invention is furture elucidated, following embodiments are only that
Illustrate the present invention without limiting the present invention.
Embodiment 1
A kind of biological agent of prevention wheat powdery mildew, raw material group become：20 parts of potato extract, 3 parts of sodium butyrate, fat
8 parts of fat alcohol, 13 parts of polyoxyethylene sorbitol acid anhydride ester, 2 parts of D-sorbite, 2 parts of ammonium sulfate, 1.0 parts of polyvinyl alcohol；
Potato extract is extracted using following methods：Skin and flesh is livid purple, germination potato is smashed, and potato is ground into
Slurry, takes 100 parts of potato pulp, and ethyl alcohol-ethylene glycol that volume is 2 times of potato pulp is then added（Volume ratio 1:1）, it is added 0.12
Part zinc chloride, filters after stirring 2h, obtains filtrate I；Water will be added in filter residue, 0.8 part of amylase is added, glucoamylase forms
Complex enzyme（The mass ratio of amylase and glucoamylase is 1:0.5）, hydrolyze 7h at 38 DEG C, 5min is stirred in enzyme deactivation at 120 DEG C
Afterwards, it dries in the air to room temperature, filtrate I and 0.1 part of methylisothiazolinone is added, continue to stir 10min, filtering obtains filtrate；
Preparation method is as follows：
（1）By sodium butyrate, fatty alcohol, polyoxyethylene sorbitol acid anhydride ester, D-sorbite, polyvinyl alcohol are uniform, obtain
Solution A；
（2）After potato extract, ammonium sulfate and polyvinyl alcohol, it is warming up to 30-35 DEG C, 30min is stirred, obtains molten
Liquid B；
（3）After solution A is mixed with solution B, after stirring 30min under conditions of 1500r/min.
Embodiment 2
A kind of biological agent of prevention wheat powdery mildew, raw material group become：26 parts of potato extract, 5 parts of sodium butyrate, fat
9 parts of fat alcohol, 15 parts of polyoxyethylene sorbitol acid anhydride ester, 3 parts of D-sorbite, 2 parts of ammonium sulfate, 0.8 part of polyvinyl alcohol；
Potato extract is extracted using following methods：Skin and flesh is livid purple, germination potato is smashed, and potato is ground into
Slurry, takes 100 parts of potato pulp, and ethyl alcohol-ethylene glycol that volume is 2 times of potato pulp is then added（Volume ratio 1:1）, it is added 0.15
Part zinc chloride, filters after stirring 2h, obtains filtrate I；Water will be added in filter residue, 0.65 part of amylase, glucoamylase composition is added
Complex enzyme（The mass ratio of amylase and glucoamylase is 1:0.5）, hydrolyze 8h at 36 DEG C, enzyme deactivation at 120 DEG C, stirring
It after 5min, dries in the air to room temperature, filtrate I and 0.1 part of methylisothiazolinone is added, continue to stir 10min, filtering obtains filtrate；
The preparation method is the same as that of Example 1 for biological agent.
Embodiment 3
A kind of biological agent of prevention wheat powdery mildew, raw material group become：30 parts of potato extract, 2 parts of sodium butyrate, fat
10 parts of fat alcohol, 10 parts of polyoxyethylene sorbitol acid anhydride ester, 3 parts of D-sorbite, 1 part of ammonium sulfate, 1.2 parts of polyvinyl alcohol.
Potato extract is extracted using following methods：Skin and flesh is livid purple, germination potato is smashed, and potato is ground into
Slurry, takes 100 parts of potato pulp, and ethyl alcohol-ethylene glycol that volume is 2 times of potato pulp is then added（Volume ratio 1:1）, it is added 0.14
Part zinc chloride, filters after stirring 2h, obtains filtrate I；Water will be added in filter residue, 0.5 part of amylase is added, glucoamylase forms
Complex enzyme（The mass ratio of amylase and glucoamylase is 1:0.5）, hydrolyze 8h at 38 DEG C, 5min is stirred in enzyme deactivation at 120 DEG C
Afterwards, it dries in the air to room temperature, filtrate I and 0.1 part of methylisothiazolinone is added, continue to stir 10min, filtering obtains filtrate；
The preparation method is the same as that of Example 1 for biological agent.
Comparative example 1
A kind of biological agent of prevention wheat powdery mildew, raw material group become：20 parts of potato extract, 3 parts of sodium butyrate, fat
8 parts of fat alcohol, 13 parts of polyoxyethylene sorbitol acid anhydride ester, 2 parts of D-sorbite, 2 parts of ammonium sulfate；
Potato extract is extracted using following methods：Skin and flesh is livid purple, germination potato is smashed, and potato is ground into
Slurry, takes 100 parts of potato pulp, and ethyl alcohol-ethylene glycol that volume is 2 times of potato pulp is then added（Volume ratio 1:1）, after stirring 2h
Filtering, it is extract to obtain filtrate；
The preparation method is the same as that of Example 1.
Comparative example 2
A kind of biological agent of prevention wheat powdery mildew, raw material group become：20 parts of potato extract, 3 parts of sodium butyrate, fat
7 parts of fat alcohol, 16 parts of polyoxyethylene sorbitol acid anhydride ester, 1 part of D-sorbite, 3 parts of ammonium sulfate, 0.6 part of polyvinyl alcohol.
Effect example：
1, the medicament for preparing embodiment 1-3 and comparative example 1 carries out performance detection, heat storage stability, cold storage stability root
It is detected according to GB/T 19136-2003, concrete outcome is shown in Table 1.
Table 1
From table 1 it follows that its heat storage stability of formula provided by the invention, cold storage stability are preferable, and exceed
After the parts by weight content of other additives provided by the invention, discovery will appear flocculation, newborn phenomena such as analysing.
2, embodiment 1-3 and comparative example the 1-2 medicament prepared are subjected to the test of pesticide effectiveness, experiment setting control group is relatively implemented
Example 1, control group 1 only contain potato extract, and sodium butyrate parts by weight use potato extract polishing；Control group 2 only with
The parts by weight of sodium butyrate, potato extract carry out polishing using ethyl alcohol-ethylene glycol；Contrast test group, contrast test group are set
Using 17.2% pyraclostrobin epoxiconazole suspension emulsion.
Experimental plot：The field for choosing Dezhou City locality wheat powdery mildew initial stage, is divided into multiple regions, each by experimental plot
About 1 mu of the area in region has field barrier, random alignment to be handled using above-mentioned medicament per minizone, and initial stage is applied
Medicine uses 2 times；
Test method：It is sprayed using -16 type knapsack hand sprayer cauline leaf of workers and peasants, is watered 750L by per hectare preparation and matches
It sets, investigation method is according to standard GB/T 17980.19-2000, and 7d is investigated before medicine and after medicine, calculates control effect, tool
Body the results are shown in Table 2.
Preventive effect computational methods are：
Above-described check plot is blank district, does not spray medicament.
Table 2
3, after being prevented for area above, the per mu yield in each experimental plot is counted, by being uninfected by wheat powdery mildew
Wheat carry out yield comparison, reduce about 3-5% using the experimental plot yield of embodiment 1-3, and comparative example 1-2 yield reduces
10% or so, and contrast test group then reduces 12% or so.
4, the present invention in potato extract with sodium butyrate ratio be 4-15:1, potato extract is denoted as A, butyric acid
Sodium is denoted as B, and each medicament is set a series of concentration gradient processing by active constituent content respectively.Reference《Farm-chemical indoor determination
Test rule fungicide》It carries out, specific toxic effect ratio calculation formula is as follows：
Toxic effect ratio=practical inhibiting rate/expection inhibiting rate；
When toxic effect ratio is more than 1.25, then synergistic effect is showed, toxic effect ratio shows antagonism when being less than 0.75, poison
It is then summation action to imitate when ratio is 1 or so, and specific toxic effect ratio is shown in Table 3.
Table 3
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not limited by embodiment
System, other any changes made without departing from the spirit and principles of the present invention, modification, combination, replacement, simplification should be
Equivalence replacement mode, is included within the scope of the present invention.
Claims (8)
1. a kind of biological agent of prevention wheat powdery mildew, which is characterized in that be made of the raw material of following parts by weight：
20-30 parts of potato extract, 2-5 parts of sodium butyrate, 8-10 parts of fatty alcohol, 10-15 parts of polyoxyethylene sorbitol acid anhydride ester,
2-3 parts of D-sorbite, 1-2 parts of ammonium sulfate, 0.8-1.2 parts of polyvinyl alcohol；
The potato extract is extracted using following methods：Skin and flesh is livid purple, germination potato is smashed, and potato is ground into
Slurry, is then added ethyl alcohol-ethylene glycol, and zinc chloride filters after stirring 2h, obtains filtrate I；To be added water in filter residue, be added amylase,
The complex enzyme of glucoamylase composition, hydrolyzes 7-8h at 36-38 DEG C, and enzyme deactivation at 120 DEG C is dried in the air to room temperature, added after stirring 5min
Enter filtrate I and methylisothiazolinone, continues to stir 10min, filtering obtains filtrate.
2. biological agent according to claim 1, which is characterized in that the volume of the same ethyl alcohol-ethylene glycol of potato pulp
Than being 1:2；The ethyl alcohol is 1 with the volume ratio of ethylene glycol:1.
3. biological agent according to claim 2, which is characterized in that the addition of the zinc chloride accounts for potato pulp weight
0.12-0.15%.
4. biological agent according to claim 1, which is characterized in that the filter residue is 1 with the mass ratio of water:2.
5. biological agent according to claim 1, which is characterized in that the addition of the complex enzyme accounts for potato weight
0.5-0.8%。
6. biological agent according to claim 5, which is characterized in that the mass ratio of the amylase and glucoamylase is
1:0.5。
7. biological agent according to claim 1, which is characterized in that the addition of the methylisothiazolinone accounts for Ma Ling
Potato starches the 0.1% of weight.
8. a kind of preparation method of such as claim 1-7 any one of them biological agents, which is characterized in that specific steps are such as
Under：
（1）By sodium butyrate, fatty alcohol, polyoxyethylene sorbitol acid anhydride ester, D-sorbite, polyvinyl alcohol are uniform, obtain solution
A；
（2）After potato extract, ammonium sulfate and polyvinyl alcohol, it is warming up to 30-35 DEG C, 30min is stirred, obtains solution B；
（3）After solution A is mixed with solution B, after stirring 30min under conditions of 1500r/min.
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| CN201610830036.1A CN106472595B (en) | 2016-09-19 | 2016-09-19 | A kind of biological agent and preparation method thereof of prevention wheat powdery mildew |
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| CN201610830036.1A CN106472595B (en) | 2016-09-19 | 2016-09-19 | A kind of biological agent and preparation method thereof of prevention wheat powdery mildew |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032246A (en) * | 2007-02-06 | 2007-09-12 | 东北师范大学 | Application of glycoalkaloids as pesticides for controlling plant diseases |
| CN102701841A (en) * | 2012-05-22 | 2012-10-03 | 芜湖市多威农化有限责任公司 | Biological antimicrobial chelerythrine aqueous emulsion and preparation method thereof |
| CN103190400A (en) * | 2013-03-15 | 2013-07-10 | 中国农业大学 | New application of sodium butyrate for improving resistance of plant to powdery mildew |
| CN104370623A (en) * | 2014-07-30 | 2015-02-25 | 青岛亚星化肥有限公司 | Potato special-purpose fertilizer and manufacturing and using methods thereof |
-
2016
- 2016-09-19 CN CN201610830036.1A patent/CN106472595B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032246A (en) * | 2007-02-06 | 2007-09-12 | 东北师范大学 | Application of glycoalkaloids as pesticides for controlling plant diseases |
| CN102701841A (en) * | 2012-05-22 | 2012-10-03 | 芜湖市多威农化有限责任公司 | Biological antimicrobial chelerythrine aqueous emulsion and preparation method thereof |
| CN103190400A (en) * | 2013-03-15 | 2013-07-10 | 中国农业大学 | New application of sodium butyrate for improving resistance of plant to powdery mildew |
| CN104370623A (en) * | 2014-07-30 | 2015-02-25 | 青岛亚星化肥有限公司 | Potato special-purpose fertilizer and manufacturing and using methods thereof |
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